CN112569277A - Coptis chinensis acne-removing composition and application thereof - Google Patents

Abstract

A composition containing Coptidis rhizoma for removing acne is provided. The paint comprises the following components in parts by weight: 0.01-10 parts of coptis chinensis extract, 0.01-10 parts of rhubarb extract, 0.01-10 parts of salvia miltiorrhiza extract, 0-10 parts of supramolecular salicylic acid, 0-10 parts of glycolic acid, 0.1-5 parts of linoleic acid, 0.01-5 parts of vitamin A and 0.01-5 parts of vitamin E. Compared with other pure traditional Chinese medicines, the coptis acne-removing composition is simpler and more effective, has the characteristic of low dependence of the pure traditional Chinese medicine formula, can effectively remove acnes, and has the functions of resisting bacteria, diminishing inflammation, relieving itching, whitening and the like.

Description

Coptis chinensis acne-removing composition and application thereof

Technical Field

The invention belongs to a pure natural Chinese herbal medicine cosmetic compound, and particularly relates to a coptis acne-removing composition.

Background

Acne (also known as whelk) is an infectious disease of the skin caused primarily by propionibacterium acnes, and is associated with, in addition to propionibacterium, an increase in androgens and sebum, hyperkeratosis at the opening of the pilosebaceous glands, and secondary inflammation. At present, the treatment is mostly carried out by adopting external bactericidal medicines such as bactericidal tretinoin, azelaic acid, sulfur lotion and the like and assisting oral antibiotics, but because of the abuse of the existing medicines, particularly the abuse of antibiotics, the proportion of drug-resistant propionibacterium is promoted year by year, and great inconvenience is brought to the clinical treatment of acne.

Research shows that the Chinese medicinal materials coptis root and rhubarb have excellent antibacterial activity, especially the activity of inhibiting propionibacterium acnes. In essence, there are a lot of researches on the development of Chinese medicines for treating acne. In 2006, patent CN1840070A discloses an application of a pharmaceutical composition in preparing a medicine for treating acne, the medicine comprises traditional Chinese medicinal materials such as coptis chinensis, rheum officinale, dandelion, salvia miltiorrhiza, fritillaria and the like, the pharmaceutical composition only comprises pure traditional Chinese medicines, western medicine components are not contained, propionibacterium is not easy to generate drug resistance, and the curative effect on acne is obvious; 2011, patent CN102370759A discloses a compound Chinese medicinal preparation for treating acne and its preparation method, which is prepared from rhizoma Coptidis, radix Salviae Miltiorrhizae, radix Et rhizoma Rhei, radix Scutellariae, fructus forsythiae, and cortex Mori by decocting and concentrating.

Disclosure of Invention

The invention aims to provide a coptis acne-removing composition, wherein the components have a synergistic effect, have no intolerance, and can effectively treat acne and remove whelk.

The coptis acne-removing composition comprises the following components in parts by weight: 0.01-10 parts of coptis chinensis extract, 0.01-10 parts of rhubarb extract, 0.01-10 parts of salvia miltiorrhiza extract, 0-10 parts of supramolecular salicylic acid, 0-10 parts of glycolic acid, 0.1-5 parts of linoleic acid, 0.01-5 parts of vitamin A and 0.01-5 parts of vitamin E.

Preferably, the coptis acne-removing composition comprises, by weight, 0.1-3 parts of coptis extract, 0.1-3 parts of rheum officinale extract, 0.1-3 parts of salvia miltiorrhiza extract, 1-5 parts of supramolecular salicylic acid, 0.2-1 part of linoleic acid, 0.1-1 part of vitamin A and 0.1-1 part of vitamin E.

Preferably, the coptis acne-removing composition comprises, by weight, 0.1-3 parts of coptis extract, 0.1-3 parts of rheum officinale extract, 0.1-3 parts of salvia miltiorrhiza extract, 1-5 parts of supramolecular salicylic acid, 0.2-1 part of linoleic acid, 0.1-1 part of vitamin A, 0.1-1 part of vitamin E, and the balance of matrix and auxiliary materials.

Preferably, the coptis acne-removing composition further comprises 5-10% by weight of glycolic acid.

Preferably, the coptis chinensis extract is prepared by the following method:

1) coarse extraction: pretreating Coptidis rhizoma, soaking in sulfuric acid, percolating, neutralizing percolate, concentrating, and filtering to obtain clear Coptidis rhizoma extract concentrate;

2) salting out: adding concentrated hydrochloric acid into the concentrated coptis extract, wherein the volume of the added concentrated hydrochloric acid is 0.5-1% of the volume of the concentrated coptis extract, then adding sodium chloride accounting for 5-10% of the weight of the concentrated coptis extract, precipitating for 1-24 hours, and carrying out solid-liquid separation to obtain a clear mother solution;

3) preparing a coptis extract: extracting the clarified mother liquor by using a solvent I, separating an organic phase I, continuously extracting a water phase by using a solvent II, discarding the water phase, collecting the organic phase II, and concentrating the organic phase II to obtain a coptis chinensis extract; wherein the solvent I is ethyl acetate, dichloromethane, chloroform or/and diethyl ether, and the solvent II is ethyl acetate-butanol or butanol.

Preferably, the concentration of the coptis chinensis extract concentrated solution is 0.1-5 g of coptis chinensis crude drug/mL of solution.

Preferably, the extraction with solvent I and the extraction with solvent II are performed 2 times with equal volumes of solvent.

More preferably, the volume of the concentrated hydrochloric acid added in the step (2) is 0.5 percent of the volume of the coptis chinensis extract concentrated solution, and the weight of the added sodium chloride is 5 percent of the weight of the coptis chinensis extract concentrated solution;

preferably, the rhubarb extract is prepared by the following method:

(a) coarse extraction: crushing rhubarb, adding 0.5-2 mol/L ammonia water solution with the volume 5-20 times that of the crude drug, stirring and extracting for 8-16 h at 10-60 ℃, centrifuging, extracting residues for 6-10 h under the same conditions, and combining extracting solutions;

(b) refining: adding ethanol with the amount of 20-30 times of the crude rheum officinale into the extracting solution, uniformly stirring, refrigerating in a refrigerator, centrifuging, and concentrating the supernatant to obtain a rheum officinale extract concentrated solution with the concentration of 0.1-3 g crude rheum officinale/mL;

(c) and (3) extracting and preparing rhubarb: adding 1-3 mol/L HCl solution into the concentrated rhubarb extract to adjust the pH value to 2-3, carrying out acid hydrolysis at 60-80 ℃ for 2-3 h, filtering and recovering the precipitate, and drying the precipitate in an oven to obtain the rhubarb extract.

Preferably, the salvia miltiorrhiza extract is prepared by the following method:

firstly, percolation extraction: crushing the salvia miltiorrhiza, soaking the salvia miltiorrhiza with ethanol with the volume of more than 75% overnight, performing percolation extraction, collecting percolate with the volume of 1-3 times that of the salvia miltiorrhiza every day, performing percolation for 5 days, stopping percolation, combining the percolates, and concentrating the percolates to obtain a total extract;

extraction: dissolving 1 part of salvia miltiorrhiza extract in 20 parts of (W/V) mixed solvent, extracting, separating an organic phase, adding anhydrous sodium sulfate, drying, placing at 0-8 ℃ for 2-10 h, performing solid-liquid separation, and concentrating the liquid to obtain the salvia miltiorrhiza extract, wherein the mixed solvent is a solvent mixed by 25-45 parts of ethyl acetate and 50 parts of sodium bicarbonate solution with the mass concentration of 5%.

The application of the coptis acne-removing composition in preparing medicines, skin care products or cosmetics for treating and/or preventing acne also belongs to the protection scope of the invention.

The coptis acne-removing composition can be directly used as a medicine for external application after being ground, or can be used as an active ingredient to be further prepared into external medicines of different formulations, including paste, medical dressing, liquid preparation, patch, spray and the like.

When the ointment is applied to the ointment, white vaseline, glyceryl monostearate, span 60, tween 80, triethanolamine, glycerol, poloxamer, fatty alcohol-polyoxyethylene ether ammonium sulfate and the like are preferably selected as the ointment matrix and auxiliary materials.

Poloxamer and fatty alcohol-polyoxyethylene ether ammonium sulfate are more preferable.

The coptis acne-removing composition can also be added into skin care products as an active ingredient, is applied after being mixed by an aqueous solvent or/and an alcoholic solvent, and comprises the steps of preparing a facial cleanser, a facial mask, cream, a cream product or essence with the skin care effect and the like, and the coptis acne-removing composition is preferably added into a hydrogel matrix/polyethylene glycol matrix to prepare the facial cleanser, the facial mask, the cream, the product or essence with the skin care effect and the like. Further, polyhydroxy acid and/or aldobionic acid can be added to make into the product.

The variety of hydrogel matrices is numerous: for example, the cross-linked water-based acrylic acid copolymer, namely carbomer resin, namely water-based thickening resin, can also adopt hydrogel formed by dissolving sodium alginate, agar and gelatin. The prepared hydrogel dressing is a clean, convenient and self-dissolving and hydrating water-based dressing. The hydrogel dressing releases the drug and is sterilized at the same time, and has a slow release function. The polymer hydrogel is generally prepared by adding a hydrophilic polymerization monomer (such as hydroxyethyl methacrylate and the like) into a suspension, adding an initiator, and rapidly catalyzing polymerization. Or polymerizing non-hydrophilic monomer such as butyl methacrylate, etc. and making into composite with hydrogel.

The coptis acne-removing composition can also be added into cosmetics as an effective component to prevent acne, relieve the problems of acne or acne stuffiness and the like caused by air impermeability and long makeup time of facial cosmetics, and is particularly applied to cream and cream products, such as foundation liquid, isolation cream, BB cream, CC cream or pre-makeup milk.

Based on the review of a large amount of documents, more than 100 Chinese medicinal materials and Chinese medicinal material prescriptions with the acne treatment effect are combed, 20 commonly used Chinese medicinal materials for treating acne are finally selected as research materials, and after a plurality of experimental researches, the composition of the invention is finally obtained, wherein the composition can remarkably synergistically inhibit epidermal cell androgen and androgen receptors, has remarkable anti-acne acetone bacillus effect, and is supplemented with whitening and skin-changing effective components such as supermolecule salicylic acid, glycyl acid and the like.

Compared with other pure traditional Chinese medicines, the coptis acne-removing composition is simpler and more effective, has the characteristic of low dependence of the pure traditional Chinese medicine formula, can effectively remove acnes, and has the functions of resisting bacteria, diminishing inflammation, relieving itching, whitening and the like.

Detailed Description

The salvia miltiorrhiza extract and the rhubarb extract in the invention can also be directly purchased from the commercially available salvia miltiorrhiza extract and rhubarb extract.

Example 1

10Kg of coptis extract, 0.01Kg of salvia extract, 0.01Kg of rhubarb extract, 5Kg of supramolecular salicylic acid, 0.1Kg of linoleic acid, 0.01Kg of vitamin A, 5Kg of vitamin E, ointment matrix and 79.87Kg of auxiliary materials.

Wherein the Coptidis rhizoma extract is prepared by slicing 40kg Coptidis rhizoma, soaking in 0.5% sulfuric acid for 24 hr, percolating, and collecting 40L percolate every day; neutralizing the collected percolate with lime to the pH of 2, and filtering; washing the residue with appropriate amount of water for 1 time, and mixing the filtrate and washing solution; concentrating the combined neutralized solution under reduced pressure to concentration of 0.1g original drug/mL, cooling, filtering, and centrifuging to obtain clear concentrated Coptidis rhizoma extractive solution; adding concentrated hydrochloric acid with the volume ratio of 0.5% into the concentrated extracting solution, adding sodium chloride with the weight ratio of 5% of the solution, precipitating overnight, and filtering to obtain a clear solution; extracting the solution with 400L ethyl acetate to obtain refined solution; extracting the refined solution with 400L ethyl acetate-butanol mixed solvent, and recovering solvent to obtain Coptidis rhizoma active site 10kg for treating acne.

The red sage root extract and the rhubarb extract are both products sold in the market, the main active component of the red sage root extract sold in the market is the tanshinone, and the main active component of the rhubarb is the rhein.

The coptis extract, the salvia extract, the rhubarb extract, the supramolecular salicylic acid and other materials are mixed according to a certain proportion to prepare the ointment. The product has antibacterial, antiinflammatory, antipruritic, and skin whitening effects, and can be used for treating dermatoses such as acne caused by Propionibacterium acnes.

Example 2

0.01kg of coptis extract, 10kg of salvia extract, 0.01kg of rhubarb extract, 5kg of glycolic acid, 5kg of linoleic acid, 5kg of vitamin A, 0.01kg of vitamin E, ointment matrix and 74.97kg of auxiliary materials.

Wherein the Coptidis rhizoma extract is prepared by slicing 40kg Coptidis rhizoma, soaking in 0.5% sulfuric acid for 24 hr, percolating, and collecting 40L percolate every day; neutralizing the collected percolate with lime to the pH of 2, and filtering; washing the residue with appropriate amount of water for 1 time, and mixing the filtrate and washing solution; concentrating the combined neutralized solution under reduced pressure to concentration of 0.1g original drug/mL, cooling, filtering, and centrifuging to obtain clear concentrated Coptidis rhizoma extractive solution; adding concentrated hydrochloric acid with the volume ratio of 0.5% into the concentrated extracting solution, adding sodium chloride with the weight ratio of 5% of the solution, precipitating overnight, and filtering to obtain a clear solution; extracting the solution with 400L ethyl acetate to obtain refined solution; extracting the refined solution with 400L ethyl acetate-butanol mixed solvent, and recovering solvent to obtain Coptidis rhizoma active site 10kg for treating acne.

The red sage root extract and the rhubarb extract are both products sold in the market, the main active component of the red sage root extract sold in the market is the tanshinone, and the main active component of the rhubarb is the rhein.

The coptis extract, the salvia extract, the rhubarb extract, the supramolecular salicylic acid and other materials are mixed according to a certain proportion to prepare the ointment. The product has antibacterial, antiinflammatory, antipruritic, and skin whitening effects, and can be used for treating dermatoses such as acne caused by Propionibacterium acnes.

Example 3

1kg of coptis extract, 1kg of salvia extract, 10kg of rhubarb extract, 2kg of supramolecular salicylic acid, 2kg of glycolic acid, 1kg of linoleic acid, 1kg of vitamin A, 1kg of vitamin E, ointment matrix and 81kg of auxiliary materials.

Wherein the Coptidis rhizoma extract is prepared by slicing 40kg Coptidis rhizoma, soaking in 0.5% sulfuric acid for 24 hr, percolating, and collecting 40L percolate every day; neutralizing the collected percolate with lime to the pH of 2, and filtering; washing the residue with appropriate amount of water for 1 time, and mixing the filtrate and washing solution; concentrating the combined neutralized solution under reduced pressure to concentration of 0.1g original drug/mL, cooling, filtering, and centrifuging to obtain clear concentrated Coptidis rhizoma extractive solution; adding concentrated hydrochloric acid with the volume ratio of 0.5% into the concentrated extracting solution, adding sodium chloride with the weight ratio of 5% of the solution, precipitating overnight, and filtering to obtain a clear solution; extracting the solution with 400L ethyl acetate to obtain refined solution; extracting the refined solution with 400L ethyl acetate-butanol mixed solvent, and recovering solvent to obtain Coptidis rhizoma active site 10kg for treating acne.

Pulverizing 1kg of Saviae Miltiorrhizae radix, soaking in 95% ethanol overnight, and percolating to extract; collecting 1 liter of percolate every day, and stopping percolation after collecting 5 liters of percolate; concentrating the percolate under reduced pressure to obtain total extract 0.2 kg; dissolving 0.2kg of radix salviae miltiorrhizae extract in 1L of mixed solvent (solvent mixed by 35 parts of ethyl acetate and 50 parts of sodium bicarbonate solution (5 percent)), sufficiently shaking and extracting for 1 hour, standing and layering to obtain an ethyl acetate layer; adding 5% anhydrous sodium sulfate into ethyl acetate solution, drying, standing in refrigerator at 4 deg.C for 6 hr, filtering to remove anhydrous sodium sulfate, and recovering ethyl acetate to obtain 0.1Kg of Saviae Miltiorrhizae radix extract.

Pulverizing 40kg radix et rhizoma Rhei, extracting with 400L 1mol/L ammonia water solution at 40 deg.C under stirring for 12 hr; centrifuging, and extracting the residue with 400L of 1mol/L ammonia water solution at 40 deg.C for 8 hr; mixing extractive solutions, adding 1200L ethanol into the extractive solution, and refrigerating in refrigerator overnight; centrifuging, and concentrating the supernatant to 1g crude rhubarb/mL; adding 2mol/L HCl solution to adjust the pH value to 2-3, hydrolyzing with acid at 70 ℃ for 3h, filtering and recovering the precipitate, and drying the precipitate in an oven at 40 ℃ to obtain 10kg of the rhubarb extract.

The coptis extract, the salvia extract, the rhubarb extract, the supramolecular salicylic acid and other materials are mixed according to a certain proportion to prepare the ointment. The product has antibacterial, antiinflammatory, antipruritic, and skin whitening effects, and can be used for treating dermatoses such as acne caused by Propionibacterium acnes.

Example 4

5kg of coptis extract, 5kg of salvia extract, 5kg of rhubarb extract, 2kg of supramolecular salicylic acid, 2kg of glycolic acid, 1kg of linoleic acid, 1kg of vitamin A, 1kg of vitamin E, paste substrate and 78kg of auxiliary materials.

The preparation method of the coptis extract comprises the following steps: pulverizing 40Kg Coptidis rhizoma, soaking in 0.5% sulfuric acid for 24 hr, percolating, and collecting 40L percolate every day; neutralizing the collected percolate with lime to the pH of 5, and filtering; washing the residue with appropriate amount of water for 2 times, and mixing the filtrate and washing solution; concentrating the combined neutralized solution under reduced pressure to a concentration of 1g original drug/mL, cooling, filtering, and centrifuging to obtain a clear concentrated solution; adding concentrated hydrochloric acid with the volume ratio of 0.5% into the concentrated clear solution, adding sodium chloride with the weight ratio of 5% of the solution, precipitating overnight, and filtering to obtain clear solution; extracting the solution with 10L of chloroform to obtain a refined solution; extracting the refined solution with 10L butanol mixed solvent, and recovering solvent to obtain Coptidis rhizoma active site 10kg for treating acne.

The preparation method of the salvia miltiorrhiza extract comprises the following steps: pulverizing 50kg of Saviae Miltiorrhizae radix, soaking in 95% ethanol overnight, and percolating to extract; collecting 50 liters of percolate every day, and stopping percolation after collecting 250 liters of percolate; concentrating the percolate under reduced pressure to obtain total extract 10 kg; dissolving 10kg of radix salviae miltiorrhizae extract in 200L of mixed solvent (solvent mixed by 35 parts of ethyl acetate and 50 parts of sodium bicarbonate solution (5 percent)), sufficiently shaking and extracting for 1 hour, standing and layering to obtain an ethyl acetate layer; adding 5% anhydrous sodium sulfate into ethyl acetate solution, drying, standing in refrigerator at 4 deg.C for 6 hr, filtering to remove anhydrous sodium sulfate, and recovering ethyl acetate to obtain active fraction of Saviae Miltiorrhizae radix for treating acne 10 kg.

The preparation method of the rhubarb extract comprises the following steps: pulverizing 40kg radix et rhizoma Rhei, extracting with 400L 1mol/L ammonia water solution at 40 deg.C under stirring for 12 hr; centrifuging, and extracting the residue with 400L of 1mol/L ammonia water solution at 40 deg.C for 8 hr; mixing extractive solutions, adding 1200L ethanol into the extractive solution, and refrigerating in refrigerator overnight; centrifuging, and concentrating the supernatant to 1g crude rhubarb/mL; adding 2mol/L HCl solution to adjust the pH value to 2-3, hydrolyzing with acid at 70 ℃ for 3h, filtering and recovering the precipitate, and drying the precipitate in an oven at 40 ℃ to obtain 10kg of active site of rhubarb for treating acne.

The coptis extract, the salvia extract, the rhubarb extract, the supramolecular salicylic acid and other materials are mixed according to a certain proportion to prepare the ointment. The product has antibacterial, antiinflammatory, antipruritic, and skin whitening effects, and can be used for treating dermatoses such as acne caused by Propionibacterium acnes.

In order to further research the anti-propionibacterium acnes and the clinical treatment effect on acnes of the coptis acne-removing composition, a plurality of groups of control groups are provided. The acne-removing composition in each control group comprises the following components in percentage by weight:

control group 1: the medicine comprises the following components: 12.5kg of coptis extract and 87.5kg of ointment base. Mixing the above materials at a certain proportion, and making into unguent.

Control group 2: the medicine comprises the following components: 6.25kg of coptis extract, 6.25kg of rhubarb and 87.5kg of ointment base. Mixing the above materials at a certain proportion, and making into unguent.

Control group 3: the medicine comprises the following components: 4kg of coptis extract, 4kg of salvia extract, 4.5kg of rhubarb extract and 87.5kg of ointment base. Mixing the above materials at a certain proportion, and making into unguent.

Control group 4: the medicine comprises the following components: 3.5kg of coptis extract, 3.5kg of salvia extract, 3.5kg of rhubarb extract, 2kg of supramolecular salicylic acid and 87.5kg of ointment substrate. Mixing the above materials at a certain proportion, and making into unguent.

Control group 5: the medicine comprises the following components: 3.5kg of coptis extract, 3.5kg of salvia extract, 3.5kg of rhubarb extract, 2kg of glycolic acid and 87.5kg of ointment base. Mixing the above materials at a certain proportion, and making into unguent.

Control group 6: the medicine comprises the following components: 3kg of coptis extract, 3kg of salvia extract, 3kg of rhubarb extract, 2kg of supermolecular salicylic acid, 1.5kg of glycolic acid and 87.5kg of ointment substrate. Mixing the above materials at a certain proportion, and making into unguent.

Control group 7: the medicine comprises the following components: 3kg of coptis extract, 3kg of salvia extract, 3kg of rhubarb extract, 1.5kg of supermolecule salicylic acid, 1kg of glycolic acid, 1kg of linoleic acid and 87.5kg of paste substrate. Mixing the above materials at a certain proportion, and making into unguent.

Control group 8: the medicine comprises the following components: 5kg of coptis extract, 5kg of salvia extract, 2kg of rhubarb extract, 5kg of pearl, 5kg of linoleic acid, 2kg of vitamin A, 2kg of vitamin E, 10kg of glycolic acid, 0.1kg of trimethoprim and 63.9kg of ointment substrate. Mixing the above materials at a certain proportion, and making into unguent.

Comparison of Effect against Propionibacterium acnes

Propionibacterium acnes is the primary pathogen that causes acne. The antibacterial experimental method is carried out according to the research guiding principle of natural medicines (traditional Chinese medicines) and new medicines: propionibacterium acnes is used as a bacterial model, and the antibacterial activity of the Propionibacterium acnes is researched by detecting MIC: after the bacteria were cultured, the concentration of the bacteria was adjusted to 1X 10⁸Per mL; 50 microliters of bacteria were added to each well of a 96-well plate, and then drugs at different concentrations were added, and the bacteria were cultured for 24 hours, and the growth of the bacteria was observed, and the MIC was determined, and the results are shown in Table 1.

TABLE 1 comparison of antibacterial (Propionibacterium acnes) effectiveness (MIC. mu.g/mL)

As can be seen from table 1, the coptis acne-removing composition of the present invention has a very good effect of inhibiting the activity of propionibacterium acnes; the product prepared by rhubarb extract and salvia extract purchased from the market also has the same curative effect; along with the increase of the number of the compatible medicines, the antibacterial activity of the composition is increased, and particularly after the coptis extract, the rhubarb extract and the salvia extract are compounded, the antibacterial effect of the rest groups except the control groups 1 and 2 is obviously increased. It was also found that control 9 was most active against propionibacterium acnes, which may be related to control 9 containing the antibiotic (trimethoprim).

Comparison of clinical therapeutic effects

The treatment method comprises the following steps: the patients in the volunteer test group were given topical facial drug and control drug once a day, in the morning and evening, and the patients were tested for 6 days.

The therapeutic effect judgment standard is as follows: the curative effect is judged according to the reduction of skin lesions (acne, pimple, pustule, etc.) before and after treatment. And (3) curing: the skin damage is reduced by more than or equal to 90 percent, no new skin damage appears, and only a small amount of pigmentation is left; the effect is shown: the skin damage is reduced by 60 to 89 percent, no new skin damage appears, and a small amount of pigmentation is left; improvement: the skin lesion is reduced by 30 to 59 percent, and a little pigmentation is left after new skin lesion appears occasionally; and (4) invalidation: the lesions subsided by less than 30%, new lesions still appeared, leaving behind pigmentation. Effective rate is (number of cure cases + number of effective cases) ÷ total number of cases × 100%. The results are shown in Table 2.

TABLE 2 Observation of clinical efficacy

Clinical statistics, the effective rate means the sum of cure, obvious effect and improvement. As can be seen from Table 2, the coptis acne-removing cream has the cure rate of 95 percent and the effective rate of 10 to 0 percent. The coptis extract is used singly, so the acne treatment effect is also achieved (the effective rate is 45%); the coptis extract and the rhubarb extract are mixed for use, so that the acne removing effect is improved (the effective rate is 55%); the coptis extract, the rhubarb extract and the salvia extract are compounded for use, so that the effect is further improved (the effective rate is 75%); especially, supermolecule salicylic acid or/and glycolic acid is added into the coptis extract, the rhubarb extract and the salvia extract, so that the curative effect is remarkably improved (the effective rate is more than 90 percent); the curative effect is higher (the effective rate is 100%) after linoleic acid and vitamin are further added. Meanwhile, the activity of resisting propionibacterium acnes of the control group 8 is better than that of the coptis acne-removing composition, but the effect of treating acne by the coptis acne-removing composition is better than that of the control group 8 (the cure rate and the effective rate of the medicine group are both better than those of the control group 8).

Claims (10)

1. The coptis acne-removing composition comprises the following components in parts by weight: 0.01-10 parts of coptis chinensis extract, 0.01-10 parts of rhubarb extract, 0.01-10 parts of salvia miltiorrhiza extract, 0-10 parts of supramolecular salicylic acid, 0-10 parts of glycolic acid, 0.1-5 parts of linoleic acid, 0.01-5 parts of vitamin A and 0.01-5 parts of vitamin E.

2. The coptis acne-removing composition according to claim 1, which comprises the following components in percentage by weight: 0.1-3 parts of coptis chinensis extract, 0.1-3 parts of rhubarb extract, 0.1-3 parts of salvia miltiorrhiza extract, 1-5 parts of supramolecular salicylic acid, 0.2-1 part of linoleic acid, 0.1-1 part of vitamin A and 0.1-1 part of vitamin E; more preferably, the pharmaceutical composition further comprises a balance of a matrix and an auxiliary material by weight.

3. The coptis acne-removing composition according to claim 2, further comprising 5-10% by weight of glycolic acid.

4. The coptis acne-removing composition according to any one of claims 1 to 3, wherein the coptis extract is prepared by the following method:

1) coarse extraction: pretreating Coptidis rhizoma, soaking in sulfuric acid, percolating, neutralizing percolate, concentrating, and filtering to obtain clear Coptidis rhizoma extract concentrate;

2) salting out: adding concentrated hydrochloric acid into the concentrated coptis extract, wherein the volume of the added concentrated hydrochloric acid is 0.5-1% of the volume of the concentrated coptis extract, then adding sodium chloride accounting for 5-10% of the weight of the concentrated coptis extract, precipitating for 1-24 hours, and carrying out solid-liquid separation to obtain a clear mother solution;

3) preparing a coptis extract: extracting the clarified mother liquor by using a solvent I, separating an organic phase I, continuously extracting a water phase by using a solvent II, discarding the water phase, collecting the organic phase II, and concentrating the organic phase II to obtain a coptis chinensis extract; wherein the solvent I is ethyl acetate, dichloromethane, chloroform or/and diethyl ether, and the solvent II is ethyl acetate-butanol or butanol.

5. The coptis acne-removing composition according to claim 4, wherein the concentration of the coptis concentrated extract is 0.1-5 g of coptis crude drug/mL of solution.

6. The coptis acne-removing composition according to claim 5, wherein the extraction with the solvent I and the extraction with the solvent II are performed for 2 times of equal volume of solvent extraction.

7. The pharmaceutical composition according to claim 5, wherein the volume of the concentrated hydrochloric acid added in the step (2) is 0.5% of the volume of the coptis chinensis extract concentrate, and the weight of the added sodium chloride is 5% of the weight of the coptis chinensis extract concentrate.

8. The coptis acne-removing composition according to any one of claims 1 to 7, wherein the rhubarb extract is prepared by the following method:

(a) coarse extraction: crushing rhubarb, adding 0.5-2 mol/L ammonia water solution with the volume 5-20 times that of the crude drug, stirring and extracting for 8-16 h at 10-60 ℃, centrifuging, extracting residues for 6-10 h under the same conditions, and combining extracting solutions;

(b) refining: adding ethanol with the amount of 20-30 times of the crude rheum officinale into the extracting solution, uniformly stirring, refrigerating in a refrigerator, centrifuging, and concentrating the supernatant to obtain a rheum officinale extract concentrated solution with the concentration of 0.1-3 g crude rheum officinale/mL;

(c) and (3) extracting and preparing rhubarb: adding 1-3 mol/L HCl solution into the concentrated extract of rhubarb to adjust the pH to 2-3

Hydrolyzing the rhubarb by acid at the temperature of 60-80 ℃ for 2-3 h, filtering and recovering the precipitate, and drying the precipitate in an oven to obtain the rhubarb extract.

9. The coptis acne-removing composition according to any one of claims 1 to 7, wherein the salvia miltiorrhiza extract is prepared by the following method:

firstly, percolation extraction: crushing the salvia miltiorrhiza, soaking the salvia miltiorrhiza with ethanol with the volume of more than 75% overnight, performing percolation extraction, collecting percolate with the volume of 1-3 times that of the salvia miltiorrhiza every day, performing percolation for 5 days, stopping percolation, combining the percolates, and concentrating the percolates to obtain a total extract;

extraction: dissolving 1 part of salvia miltiorrhiza extract in 20 parts of (W/V) mixed solvent, extracting, separating an organic phase, adding anhydrous sodium sulfate, drying, placing at 0-8 ℃ for 2-10 h, performing solid-liquid separation, and concentrating the liquid to obtain the salvia miltiorrhiza extract, wherein the mixed solvent is a solvent mixed by 25-45 parts of ethyl acetate and 50 parts of sodium bicarbonate solution with the mass concentration of 5%.

10. Use of the coptis acne-removing composition as defined in any one of claims 1 to 9 in preparation of medicines, skin care products or cosmetics for treating and/or preventing acne.