CN112546078A - Ganoderma lucidum spore extract and application thereof - Google Patents
Ganoderma lucidum spore extract and application thereof Download PDFInfo
- Publication number
- CN112546078A CN112546078A CN202011348354.7A CN202011348354A CN112546078A CN 112546078 A CN112546078 A CN 112546078A CN 202011348354 A CN202011348354 A CN 202011348354A CN 112546078 A CN112546078 A CN 112546078A
- Authority
- CN
- China
- Prior art keywords
- ganoderma lucidum
- lucidum spore
- extract
- extraction
- spore powder
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 240000008397 Ganoderma lucidum Species 0.000 title claims abstract description 48
- 235000001637 Ganoderma lucidum Nutrition 0.000 title claims abstract description 48
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 44
- 239000000843 powder Substances 0.000 claims abstract description 37
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 18
- 239000000706 filtrate Substances 0.000 claims abstract description 16
- 239000006228 supernatant Substances 0.000 claims abstract description 14
- 238000001914 filtration Methods 0.000 claims abstract description 12
- 241000222336 Ganoderma Species 0.000 claims abstract description 10
- 238000000034 method Methods 0.000 claims abstract description 9
- 239000000341 volatile oil Substances 0.000 claims abstract description 9
- 238000001556 precipitation Methods 0.000 claims abstract description 8
- 238000010298 pulverizing process Methods 0.000 claims abstract description 5
- 210000002421 cell wall Anatomy 0.000 claims abstract description 4
- 238000002156 mixing Methods 0.000 claims abstract description 4
- 238000000605 extraction Methods 0.000 claims description 15
- 238000003756 stirring Methods 0.000 claims description 7
- 238000005238 degreasing Methods 0.000 claims description 6
- 239000012141 concentrate Substances 0.000 claims description 5
- 239000003814 drug Substances 0.000 claims description 5
- 238000003809 water extraction Methods 0.000 claims description 4
- 230000029087 digestion Effects 0.000 claims description 3
- 230000001737 promoting effect Effects 0.000 claims description 3
- 238000002360 preparation method Methods 0.000 claims description 2
- 239000000203 mixture Substances 0.000 claims 1
- 150000004676 glycans Chemical class 0.000 abstract description 6
- 238000004519 manufacturing process Methods 0.000 abstract description 6
- 229920001282 polysaccharide Polymers 0.000 abstract description 6
- 239000005017 polysaccharide Substances 0.000 abstract description 6
- 239000003921 oil Substances 0.000 abstract description 4
- 239000000047 product Substances 0.000 abstract description 4
- 230000007613 environmental effect Effects 0.000 abstract description 3
- 241000699670 Mus sp. Species 0.000 description 8
- 230000000694 effects Effects 0.000 description 8
- 230000037396 body weight Effects 0.000 description 5
- 235000013305 food Nutrition 0.000 description 5
- 238000001514 detection method Methods 0.000 description 4
- 238000002474 experimental method Methods 0.000 description 4
- 235000012631 food intake Nutrition 0.000 description 4
- 230000037406 food intake Effects 0.000 description 4
- 230000000968 intestinal effect Effects 0.000 description 4
- 241001465754 Metazoa Species 0.000 description 3
- 230000008901 benefit Effects 0.000 description 3
- -1 compound diphenoxylate Chemical class 0.000 description 3
- 229960004192 diphenoxylate Drugs 0.000 description 3
- 239000012153 distilled water Substances 0.000 description 3
- 230000002708 enhancing effect Effects 0.000 description 3
- 210000001187 pylorus Anatomy 0.000 description 3
- 210000000813 small intestine Anatomy 0.000 description 3
- 235000019786 weight gain Nutrition 0.000 description 3
- 241000700159 Rattus Species 0.000 description 2
- 230000001276 controlling effect Effects 0.000 description 2
- 230000007812 deficiency Effects 0.000 description 2
- 230000006870 function Effects 0.000 description 2
- 230000002496 gastric effect Effects 0.000 description 2
- 235000013402 health food Nutrition 0.000 description 2
- 230000036039 immunity Effects 0.000 description 2
- 230000010412 perfusion Effects 0.000 description 2
- 239000002244 precipitate Substances 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 210000000952 spleen Anatomy 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 230000004584 weight gain Effects 0.000 description 2
- 208000035240 Disease Resistance Diseases 0.000 description 1
- 241001489091 Ganoderma sinense Species 0.000 description 1
- 241000699666 Mus <mouse, genus> Species 0.000 description 1
- 241000222341 Polyporaceae Species 0.000 description 1
- 210000000683 abdominal cavity Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 210000000988 bone and bone Anatomy 0.000 description 1
- 230000001914 calming effect Effects 0.000 description 1
- 238000000658 coextraction Methods 0.000 description 1
- 235000009508 confectionery Nutrition 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 230000007661 gastrointestinal function Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 230000033001 locomotion Effects 0.000 description 1
- 210000004072 lung Anatomy 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 210000000713 mesentery Anatomy 0.000 description 1
- 210000005036 nerve Anatomy 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 230000001376 precipitating effect Effects 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000001141 propulsive effect Effects 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- 238000005728 strengthening Methods 0.000 description 1
- 235000019605 sweet taste sensations Nutrition 0.000 description 1
- 210000002435 tendon Anatomy 0.000 description 1
- 150000003648 triterpenes Chemical class 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/06—Fungi, e.g. yeasts
- A61K36/07—Basidiomycota, e.g. Cryptococcus
- A61K36/074—Ganoderma
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
- A61P1/14—Prodigestives, e.g. acids, enzymes, appetite stimulants, antidyspeptics, tonics, antiflatulents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/37—Extraction at elevated pressure or temperature, e.g. pressurized solvent extraction [PSE], supercritical carbon dioxide extraction or subcritical water extraction
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/53—Liquid-solid separation, e.g. centrifugation, sedimentation or crystallization
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/50—Improvements relating to the production of bulk chemicals
- Y02P20/54—Improvements relating to the production of bulk chemicals using solvents, e.g. supercritical solvents or ionic liquids
Landscapes
- Health & Medical Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Life Sciences & Earth Sciences (AREA)
- Pharmacology & Pharmacy (AREA)
- Mycology (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Animal Behavior & Ethology (AREA)
- Alternative & Traditional Medicine (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Microbiology (AREA)
- Medical Informatics (AREA)
- Botany (AREA)
- Biotechnology (AREA)
- Nutrition Science (AREA)
- Epidemiology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Organic Chemistry (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Medicines Containing Plant Substances (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a ganoderma lucidum spore extract and application thereof, wherein the ganoderma lucidum spore extract is prepared by the following method: breaking cell wall of Ganoderma spore powder, pulverizing, defatting to obtain defatted spore powder and volatile oil, extracting the defatted spore powder with water, filtering, mixing filtrates, concentrating the filtrate, adding ethanol, standing for precipitation, filtering to obtain supernatant, recovering ethanol from the supernatant, and concentrating to relative density of 1.1-1.2 to obtain extract. The invention fully utilizes the residual products of the production of the ganoderma lucidum spore oil and the ganoderma lucidum spore polysaccharide, carries out all-round development on the ganoderma lucidum spore powder, accords with the concept of environmental protection, and simultaneously obviously reduces the production cost of enterprises.
Description
Technical Field
The invention belongs to the technical field of biology, and particularly relates to a ganoderma lucidum spore extract and application thereof.
Background
Ganoderma is fruiting body of Ganoderma lucidum or Ganoderma sinense belonging to Polyporaceae. Sweet in taste and neutral in nature, has the functions of replenishing qi and blood, calming the heart and invigorating the spleen and stomach. It is listed as the superior product in Shen nong Ben Cao Jing (Shen nong's herbal). It is said to benefit heart-qi, tonify middle energizer, increase intelligence without forgetting, keep spirit, benefit vital essence and energy, benefit joints and strengthen tendons and bones. Has the functions of reducing weight and prolonging life after long-time taking. The indications of the classic traditional Chinese medicine are all diseases of body deficiency and strain, which indicates that the traditional Chinese medicine has the special effect of tonifying deficiency and strengthening body. Ganoderma lucidum is collected in pharmacopoeia of the people's republic of China, version 2000, and later pharmacopoeia. Modern pharmacology also recognizes the effects of enhancing non-specific immunity and enhancing disease resistance of the body.
The ganoderma lucidum spore powder is spores ejected from a pileus when ganoderma lucidum is mature. With the progress of modern scientific and technological means, the ganoderma lucidum spores are newly known. It concentrates the essence of glossy ganoderma and the vital qi of innate nature and earth, and obviously improves the efficacy and clinical application effect. The ganoderma lucidum spore powder comprises wall-broken ganoderma lucidum spore powder which is processed and standardized by traditional Chinese medicine decoction pieces in Zhejiang province, Anhui province and the like, has mild nature and sweet taste, and has various efficacies of tonifying qi, soothing nerves, invigorating spleen, benefiting lung and the like. Is also widely applied in the field of health food. Has been included in the catalog of health food record materials, and the safety and the efficacy of enhancing immunity are also widely accepted.
The currently accepted effective components of the ganoderma lucidum spore powder are polysaccharides and triterpenes, the volatile oil and the polysaccharides of the ganoderma lucidum spore powder are utilized more thoroughly, and other substances are usually discarded as waste residues. The invention fully utilizes the residual products of the production of the ganoderma lucidum spore oil and the ganoderma lucidum spore polysaccharide, carries out all-round development on the ganoderma lucidum spore powder, accords with the concept of environmental protection, and simultaneously obviously reduces the production cost of enterprises.
Disclosure of Invention
Aiming at the defects, the invention provides the ganoderma lucidum spore extract for fully utilizing the ganoderma lucidum spore powder in all directions.
The invention also aims to provide the application of the ganoderma lucidum spore extract in the aspect of promoting digestion.
The purpose of the invention is realized by the following modes:
a ganoderma lucidum spore extract is obtained by the following method: breaking the wall of ganoderma lucidum spore powder, crushing, degreasing to obtain degreased spore powder and volatile oil, extracting the degreased spore powder with water, filtering, combining the filtrates, concentrating the filtrate, adding ethanol, standing for precipitation, filtering to obtain a supernatant, recovering the ethanol from the supernatant, and concentrating to the density of 1.1-1.2 to obtain the extract.
The degreasing is performed by supercritical CO2And (3) extracting under the following extraction conditions: the pressure is 30MPa, the flow rate is 350-400L/h, the temperature is 40-45 ℃, and the time is 4-5 h.
The water extraction is carried out for 1-2 times, 8-10 times of water is added for each time, the extraction time is 6-10 hours for each time, and the extraction temperature is 70-80 ℃.
And the step of adding ethanol, standing and precipitating is to concentrate the filtrate to the density of 1.10-1.12, control the temperature to be not more than 80 ℃, slowly add 2 times of 95% ethanol by weight of the concentrated solution while stirring, and stand at 4 ℃ overnight after the addition.
The preparation method of the ganoderma lucidum spore extract comprises the following steps: breaking the wall of ganoderma lucidum spore powder, crushing, degreasing to obtain degreased spore powder and volatile oil, extracting the degreased spore powder with water, filtering, combining the filtrates, concentrating the filtrate, adding ethanol, standing for precipitation, filtering to obtain a supernatant, recovering the ethanol from the supernatant, and concentrating to the density of 1.1-12 to obtain the extract. The degreasing is carried out by supercritical CO2 extraction under the following conditions: the pressure is 30MPa, the flow rate is 350-400L/h, the temperature is 40-45 ℃, and the time is 4-5 h. The water extraction is carried out for 1-2 times, 8-10 times of water is added for each time, the extraction time is 6-10 hours for each time, and the extraction temperature is 70-80 ℃. And the step of adding ethanol and standing for precipitation is to concentrate the filtrate to the density of 1.10-1.12, control the temperature to be not more than 80 ℃, slowly add 2 times of 95% ethanol in weight concentration into the concentrated solution while stirring, and stand for overnight at 4 ℃ after the addition is finished.
The ganoderma lucidum spore extract prepared by the method has obvious effect when being applied to preparing the medicine for promoting digestion.
The invention re-enriches the substances after extracting the ganoderma lucidum spore oil and the polysaccharide, and finds that the ganoderma lucidum spore extract has obvious effect on regulating the gastrointestinal function. The process fully utilizes the residual products of the production of the ganoderma lucidum spore oil and the ganoderma lucidum spore polysaccharide, accords with the concept of environmental protection, and simultaneously obviously reduces the production cost of enterprises.
Detailed Description
The invention is further illustrated by the following specific examples
Example 1
Breaking cell wall of Ganoderma spore powder, pulverizing, granulating, and extracting with supercritical CO2Extracting, supercritical CO2The extraction conditions were: extracting defatted spore powder and volatile oil with water under 30MPa at flow rate of 400L/h and temperature of 40 deg.C for 4h for 2 times, extracting with 10 times of water at 80 deg.C for 10 hr, extracting with 8 times of water at 80 deg.C for 6 hr, filtering, mixing filtrates, vacuum concentrating to density of 1.10, controlling temperature not more than 80 deg.C, slowly adding 95% ethanol 2 times of the concentrate, stirring while adding, standing at 4 deg.C overnight, separating to obtain precipitate and supernatant, recovering ethanol from the supernatant, and concentrating to relative density of 1.2 to obtain extract. The total sugar content of the extract was determined to be 120mg/ml and the protein content was determined to be 62 mg/ml.
The detection method of the total sugar content comprises the following steps: determination of total sugar content in GB/T15672-
The detection method of the protein content comprises the following steps: determination of protein in GB 5009.5-2016 food
Example 2
Breaking cell wall of Ganoderma spore powder, pulverizing, granulating, and extracting with supercritical CO2Extracting, supercritical CO2The extraction conditions were: extracting defatted spore powder and volatile oil with water at 30MPa, flow rate of 350L/h, temperature of 45 deg.C for 5 hr for 2 times, extracting with 10 times of water at 80 deg.C for 10 hr, and collecting at the second timeExtracting with 8 times of water at 80 deg.C under stirring for 6 hr, filtering, mixing filtrates, vacuum concentrating to density of 1.10, controlling temperature not to exceed 80 deg.C, slowly adding 2 times of 95% ethanol, stirring while adding, standing at 4 deg.C overnight, separating to obtain precipitate and supernatant, recovering ethanol from the supernatant, and concentrating to relative density of 1.1 to obtain extract. The total sugar content of the extract was found to be 126mg/ml and the protein content was found to be 64 mg/ml.
The detection method of the total sugar content comprises the following steps: determination of total sugar content in GB/T15672-
The detection method of the protein content comprises the following steps: determination of protein in GB 5009.5-2016 food
The effects of the present invention are further illustrated by the following test examples:
test example 1
The mouse small intestine ink propulsion test comprises the following steps:
randomly dividing mice into blank control group, model control group, sample group (prepared according to example 1), wall-broken Ganoderma spore powder group and defatted Ganoderma spore powder group (obtained by breaking wall of Ganoderma spore powder, pulverizing, granulating, and performing supercritical CO extraction2Extracting, supercritical CO2The extraction conditions were: removing volatile oil under 30MPa, flow rate of 400L/h, temperature of 40 deg.C, and time of 4h, and collecting defatted spore powder). The sample group is administrated in a gastric perfusion mode according to 10 times of the amount recommended by adults, namely 0.5g/kg & bw, distilled water is supplied to a blank control group and a model control group, and meanwhile, wall-broken ganoderma lucidum spore powder and defatted ganoderma lucidum spore powder are administrated in a gastric perfusion mode according to 2.0g/kg & bw, 1 time per day and 30 days continuously.
After the experiment is finished, fasting is not forbidden for 16 hours, a sample group, a model, a blank control group, a wall-broken ganoderma lucidum spore powder group and a defatted ganoderma lucidum spore powder group are given with a test sample or distilled water once again on the day of determination, compound diphenoxylate is filled in each sample group and the model control group after 30min, distilled water is filled in the blank control group, an ink indicator is given in each group after 30min, the neck is cut off after 25min to kill animals, the abdominal cavity is opened to separate mesentery, the intestinal canal with the upper end from the pylorus, the lower end to the ileocecal part is cut and placed on a flat plate, the intestinal canal is slightly pulled into a straight line, the distances from the pylorus to the ileocecal part and from the pylorus to the ink front edge are respectively measured, and:
the results are shown in Table 1:
TABLE 1 Effect on intestinal propulsive locomotion in mice
Note: indicates comparison with blank control group (P <0.01) and # indicates comparison with model control group (P < 0.01).
Table 1 shows that the small intestine propulsion rate of the model control group is significantly lower than that of the blank control (P <0.01), indicating that the model is successfully established. The intestinal ink propulsion rate of the sample group mice is obviously higher than that of the compound diphenoxylate model control group (P < 0.01). The ganoderma lucidum spore extract has an improvement effect on the small intestine propulsion reduction caused by the compound diphenoxylate.
Second, animal weight, weight gain, food intake and food utilization rate determination test:
during the experiment, the sample group is administrated by intragastric administration according to the dosage of 0.5 g/kg-bw, the ganoderma lucidum spore powder group and the degreased ganoderma lucidum spore powder group according to the dosage of 2.0 g/kg-bw for 30 days continuously. Mice were measured for body weight and food intake 2 times per week and the dosage was adjusted according to changes in body weight. Animal weight gain, food intake and food utilization were calculated at the end of the experiment. The results are shown in Table 2.
TABLE 2 weight changes of mice before and after the experiment
Note: denotes P <0.05 compared to the blank control group, denotes P < 0.01.
As can be seen from Table 2, the initial body weights of the mice in the sample group are not significantly different from those of the blank control group, and the body weight gain of the mice in the sample group is obviously higher than that of the blank control group after the mice are given for 30 days.
TABLE 3 changes in rat body weight and food availability
Note: denotes P <0.05 compared to control group, denotes P < 0.01.
Table 2 shows that the total food intake of rats in the sample group is obviously higher than that of the control group (P <0.05), and the other indexes have no significant difference compared with the control group (P > 0.05).
Claims (9)
1. A ganoderma lucidum spore extract is characterized in that: breaking cell wall of Ganoderma spore powder, pulverizing, defatting to obtain defatted spore powder and volatile oil, extracting the defatted spore powder with water, filtering, mixing filtrates, concentrating the filtrate, adding ethanol, standing for precipitation, filtering to obtain supernatant, recovering ethanol from the supernatant, and concentrating to relative density of 1.1-1.2 to obtain extract.
2. The ganoderma lucidum spore extract of claim 1, wherein: the degreasing is realized by adopting supercritical CO2And (3) extracting under the following extraction conditions: the pressure is 30MPa, the flow rate is 350-400L/h, the temperature is 40-45 ℃, and the time is 4-5 h.
3. The ganoderma lucidum spore extract of claim 1, wherein: the water extraction is carried out for 1-2 times, 8-10 times of water is added for each time, the extraction time is 6-10 hours for each time, and the extraction temperature is 70-80 ℃.
4. The ganoderma lucidum spore extract as claimed in claim 1, wherein the step of adding ethanol and standing for precipitation is to concentrate the filtrate to a density of 1.10-1.12, control the temperature to be not more than 80 ℃, slowly add 2 times of 95% ethanol by weight of the concentrated solution while stirring, and stand for overnight at 4 ℃ after the addition.
5. A method for preparing the ganoderma lucidum spore extract as claimed in claim 1, characterized in that the method comprises the following steps: breaking the wall of ganoderma lucidum spore powder, crushing, degreasing to obtain degreased spore powder and volatile oil, extracting the degreased spore powder with water, filtering, combining the filtrates, concentrating the filtrate, adding ethanol, standing for precipitation, filtering to obtain a supernatant, recovering the ethanol from the supernatant, and concentrating to the density of 1.1-12 to obtain the extract.
6. The method for preparing ganoderma lucidum spore extract according to claim 5, wherein the defatting is performed by supercritical CO2 extraction under the following conditions: the pressure is 30MPa, the flow rate is 350-400L/h, the temperature is 40-45 ℃, and the time is 4-5 h.
7. The method for preparing ganoderma lucidum spore extract according to claim 5, wherein the water extraction is performed for 1-2 times, 8-10 times of water is added for each time, the extraction time is 6-10 hours for each time, and the extraction temperature is 70-80 ℃.
8. The method for preparing ganoderma lucidum spore extract according to claim 5, wherein the step of adding ethanol and standing for precipitation is that the filtrate is concentrated to a density of 1.10-1.12, the temperature is controlled to be not more than 80 ℃, 2 times of ethanol with a concentration of 95% by weight of the concentrated solution is slowly added while stirring, and the mixture is left to stand for overnight at 4 ℃ after the addition.
9. Use of the ganoderma spore extract of claim 1 in the preparation of a medicament for promoting digestion.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011348354.7A CN112546078B (en) | 2020-11-26 | 2020-11-26 | Ganoderma lucidum spore extract and application thereof |
| PCT/CN2021/130818 WO2022111331A1 (en) | 2020-11-26 | 2021-11-16 | Ganoderma lucidum spore extract and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011348354.7A CN112546078B (en) | 2020-11-26 | 2020-11-26 | Ganoderma lucidum spore extract and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN112546078A true CN112546078A (en) | 2021-03-26 |
| CN112546078B CN112546078B (en) | 2022-08-30 |
Family
ID=75046866
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202011348354.7A Active CN112546078B (en) | 2020-11-26 | 2020-11-26 | Ganoderma lucidum spore extract and application thereof |
Country Status (2)
| Country | Link |
|---|---|
| CN (1) | CN112546078B (en) |
| WO (1) | WO2022111331A1 (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113842450A (en) * | 2021-10-12 | 2021-12-28 | 南京中科药业有限公司 | Application of ganoderma lucidum spore powder polysaccharide peptide in preparation of medicine for treating cancer-induced fatigue |
| WO2022111331A1 (en) * | 2020-11-26 | 2022-06-02 | 中科健康产业集团股份有限公司 | Ganoderma lucidum spore extract and application thereof |
| CN116035149A (en) * | 2022-12-28 | 2023-05-02 | 南京中科药业有限公司 | Preparation method of ganoderma lucidum solid beverage |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116617274B (en) * | 2023-06-09 | 2024-05-03 | 云南德彩堂生物医药科技有限公司 | Wall-broken ganoderma lucidum spore powder solid dispersion, and preparation method and application thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1483743A (en) * | 2003-06-10 | 2004-03-24 | 南京中科生化技术有限公司 | Method for extracting ganoderma lucidum spore polysaccharide |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1067813A (en) * | 1991-06-25 | 1993-01-13 | 周纯喜 | The production method of Chinese medicine compound " spleen bao " |
| CN100424098C (en) * | 2006-10-25 | 2008-10-08 | 南京中科集团股份有限公司 | Process for refining glossy ganoderma spore polysaccharide |
| CN111662393A (en) * | 2020-07-01 | 2020-09-15 | 广东悦生生物科技有限公司 | Method for extracting total components of lucid ganoderma |
| CN112546078B (en) * | 2020-11-26 | 2022-08-30 | 中科健康产业集团股份有限公司 | Ganoderma lucidum spore extract and application thereof |
-
2020
- 2020-11-26 CN CN202011348354.7A patent/CN112546078B/en active Active
-
2021
- 2021-11-16 WO PCT/CN2021/130818 patent/WO2022111331A1/en active Application Filing
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1483743A (en) * | 2003-06-10 | 2004-03-24 | 南京中科生化技术有限公司 | Method for extracting ganoderma lucidum spore polysaccharide |
Non-Patent Citations (1)
| Title |
|---|
| 李志超等: "《食用菌名特产品栽培新技术》", 31 May 2005, 上海科学技术文献出版社 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2022111331A1 (en) * | 2020-11-26 | 2022-06-02 | 中科健康产业集团股份有限公司 | Ganoderma lucidum spore extract and application thereof |
| CN113842450A (en) * | 2021-10-12 | 2021-12-28 | 南京中科药业有限公司 | Application of ganoderma lucidum spore powder polysaccharide peptide in preparation of medicine for treating cancer-induced fatigue |
| CN116035149A (en) * | 2022-12-28 | 2023-05-02 | 南京中科药业有限公司 | Preparation method of ganoderma lucidum solid beverage |
Also Published As
| Publication number | Publication date |
|---|---|
| WO2022111331A1 (en) | 2022-06-02 |
| CN112546078B (en) | 2022-08-30 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN112546078B (en) | Ganoderma lucidum spore extract and application thereof | |
| CN102415568B (en) | Health food for improving immunity and preparation method thereof | |
| TWI782283B (en) | Use of walnut oligopeptide powder | |
| CN104263790B (en) | A kind of saline cistanche polypeptide extract, preparation method and its usage | |
| CN104435749B (en) | A kind of dendrobium candidum compound preparation and its preparation method and application | |
| JP5391282B2 (en) | Composition for promoting hematopoietic stem cell proliferation comprising fistula extract, reishi extract and mulberry extract | |
| CN102755346A (en) | Compound polysaccharide formula of edible fungus as well as preparation method and application thereof | |
| CN111686147A (en) | Eucommia ulmoides extract and application thereof in treating osteoporosis | |
| KR101002774B1 (en) | The invigorate energy food which is made from herb medicine and the producing method thereof | |
| CN112569305A (en) | Mushroom compound polysaccharide composition for improving immunity and preparation method thereof | |
| CN1895546B (en) | Lycium chinensis polyose medicinal composition with therapeutic and health-care functions | |
| CN106038614A (en) | A cordyceps militaris polysaccharide-edible fungus ferment compound drink and a preparing method thereof | |
| CN108771076A (en) | A kind of compound curcumin fribrillin solid beverage prepares and redissolves method | |
| CN109589400B (en) | Composition with neuroprotective effect | |
| CN109430854B (en) | Health product and preparation method thereof | |
| CN104491606B (en) | A kind of Chinese medicine composition and preparation and application thereof | |
| CN114365844B (en) | Bamboo fungus compound health food for regulating intestinal flora and improving intestinal immunity and preparation method thereof | |
| CN101897438B (en) | Nutriment with leukocyte-increasing effect and preparation method thereof | |
| CN104906140A (en) | Donkey-hide gelatin, a preparation method and associated equipment | |
| CN102784230B (en) | Pharmaceutical composition preparation for treating nutritional anemia | |
| CN109294848A (en) | A kind of active bio nanometer selenium wine and preparation method thereof | |
| CN112274619B (en) | Composition for relieving dysmenorrhea and application of composition in preparation of medicines, health-care products or foods | |
| CN110025709B (en) | Preparation method and application of exodonthus urinaria and rhizoma dioscoreae powder | |
| CN103211224A (en) | Preparation process of compound health care food capable of rapidly recovering physical strength of man | |
| CN102973725A (en) | Lentinan capsules |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |