CN1125382A - Miticidal composition and method for controlling spider mite populations - Google Patents

Abstract

A miticidal composition is provided for controlling female and male spider mite populations whether susceptible or resistant and whether immature or adult. The miticidal composition has a miticide selected from the group consisting of organotins, organophosphates, diamides, sulfite esters, macrocyclic lactones, elemental sulfur and combinations thereof; and an effective amount of a sesquiterpene compound selected from the group consisting of sesquiterpene alcohols, analogs thereof and combinations thereof for enhancing the toxic effectiveness of the selected miticide. Preferably, the effective amount of the sesquiterpene compound ranges from about 300 ppm to about 1000 ppm in the composition, with the proviso that when the miticide is elemental sulfur the effective amount of the sesquiterpene compound ranges from about 500 ppm to about 1000 ppm in the composition. The sesquiterpene compound is preferably a sesquiterpene alcohol, more preferably the sesquiterpene alcohol is selected from the group consisting of farnesol, nerolidol and combinations thereof.

Description

The method of miticide composition and control tetranychid population

Background of the present invention

1. FIELD OF THE INVENTION

The present invention relates generally to improve the chemical substance of the effectiveness of the miticide of control tetranychid or insecticide, more particularly, relate to and (for example comprise miticide or insecticide and at least a sesquiterpene alcohols or its analog, farnesol and/or nerolidol) new miticide composition, its amount should be in to the control of the female and male tetranychid of responsive and resistant population the effectiveness of effective raising miticide or insecticide.

2. the explanation of prior art

The tetranychid that belongs to mite section (Acari) formal name used at school and be Tetranychidae is a kind of common pest, the blade of their infringement crops, trees and ornamental plantss.Two kinds of common tetranychids are Tetranychus cinnabarinus (Tetranychus cinnabarinus) and two-spotted spider mite (Tetranychusurticae), and these two kinds of insects all can cause damage and the underproduction to cotton field and other crop.

Exist multiple at present by killing the miticide that tetranychid prevents that tetranychid from infecting.A kind of common miticide is fenbutatin oxide (CAS-13356-08-6), the commercially available prod is DuPontAgricultural Products's " VENDEX " (registration mark) and ShellInternational Chemical Co., " OSADAN " of Ltd. (London) and " TORQUE " (registration mark).Another kind of common miticide is an Olivomitecidin, can be from Merck ﹠amp; Co., Inc. buys, and trade mark is " AVID ", " ZEPHR " and " AGRI-MEK ".The common miticide of the third type is amitraz (CAS-33089-61-1), can be from NOR-AM Chemical Co., and Wilmington.Delaware buys, and trade mark is " MITAC " and " ORASYN ".Because above-mentioned miticide is effective to tetranychid in a short time usually, and tetranychid can breed fast, and develops in about one to three year time and the resistant strain that above-mentioned acaricidal tolerance is improved.It is generally acknowledged that having only tetranychid and most miticides to take place directly to contact just can kill it., compare with the pest of other type, the activity of tetranychid is few relatively, mainly at the leaf back cluster, therefore, covers fully guaranteeing unless use quite a large amount of above-mentioned miticides, and tetranychid is difficult for directly contacting with the miticide of using.Can cause the tangible waste except use a large amount of above-mentioned miticides to the blade of plant to be protected or trees, it is generally acknowledged that using above-mentioned miticide can quicken above-mentioned acaricidal tolerance development repeatedly heavy dose ofly.

More known compounds can improve acaricidal effectiveness., the effectiveness of above-mentioned composition generally can not be expected.For example, Japanese patent application JP 61-291501 discloses a kind of branch that contains and has pounced on chrysanthemum ester and the orthene acaricide composition as active component.It is pyrethrins type insecticide and the miticide that synthesizes that branch is pounced on the chrysanthemum ester.It is known that it is effective to tetranychid.Orthene is bad to mite class effect, and is invalid as miticide.Above-mentioned application discloses to divide and has pounced on chrysanthemum ester and orthene and fall the line mite as composition of active components control mite class such as the many foods of Tetranychus cinnabarinus, Kanzawa tetranychid, tangerine Panonychus citri and side and have excellent effect.Above-mentioned active component can combine with liquid-carrier, and liquid-carrier is alcohols (C for example _1-4Alcohol, ethylene glycol and benzylalcohol), arene, halogenated hydrocarbon, ethers, ketone, ester class, nitrile, sulfoxide class, pure ethers, aliphatic series or cycloaliphatic hydrocarbons, industrial gasoline and petroleum distillate.

The female worm of also known insect kinds and other insect can be produced some natural chemical substances that attract male worm.Above-mentioned naturally occurring property suction lure is called as pheromones.From the female worm of above-mentioned insect, extract polytype this class sex pheromone in some cases with chemical method, can prepare above-mentioned sex pheromone by synthetic method.In other cases, find that some are not that the natural chemical substance that is present in insect kind or other insect but has the effect that male worm and/or the female worm of insect or other insect is attracted, repels or its behavior is taken place other influence.Sometimes this natural existence or synthetic chemical substance are referred to as behavior adjusting material.

Material is regulated in the behavior of the natural generation of known some tetranychid, influences the behavior of tetranychid by attraction, repulsion or inhibition male worm.The research report of Regev and Cone shows that female two-spotted spider mite can produce a kind of sex pheromone, this sex pheromone can attract the male mite of this kind to rest on female regeneration pupa place, and this allure to male mite remains to female one-tenth mite always and sprouts wings, and at this moment they carry out mating usually; The extract of female tetranychid regeneration pupa shows the existence of sesquiterpene alcohols farnesol.Regev and Cone are entitled as that " farnesol is as two-spotted spider mite (Tetranychus urticae) (mite section: the evidence of male attractant " Environmental Entomology Tetranychus), in April, 1985,4 volumes, 2 phases, the research paper of 307-311 page or leaf is pointed out, under finite concentration, some isomer of synthetic farnesol has the effect of luring male tetranychid.The further research of Regev and Cone " pre-age, the nerolidol and the spiceleaf tertiary alcohol of female two-spotted spider mite were analyzed: to the evaluation of the property attracting action of male mite ", Environmental Entomology, in February, 1976,5 volumes, 1 phase, the 133-138 page or leaf has also disclosed in the female regeneration pupa of two-spotted spider mite and has also contained the sesquiterpene alcohols nerolidol; They studies show that under particular concentration, synthetic nerolidol can be lured male two-spotted spider mite.

The notion that mixed volatilization sex attractant and insecticide are prevented and treated high insect population is known.For example, people's such as Kinzer U.S. Pat 4,122,165 and Thuron Industries, Inc. Australian Patent AU477,526 all described sex pheromone suitable-9-tricosene (muscalure) and insecticide composition can prevent and treat the population of housefly (Musca domestica).The pesticide compound of the material (for example, cabreuva oil) that is mixed with farnesol or contains this liquid alcohol is disclosed in the Japanese patent gazette 41 (1966)-19198.Concentration range provides effective insecticide at 0.01 to 3% farnesol and insecticide (for example organophosphorus ester, organic chloride and natural insecticide) mixing energy.And farnesol has the incidence that postpones to pupate, reduces the pupa that pupation rate and raising can not grow fully to housefly effect disclosed.A kind of Pesticidal combination that contains basudin (5% weight), cabreuva oil (80% weight), Tween 80 (5.0% weight) and mixed xylenes (10% weight) is disclosed in the embodiment 5 of this patent.Cabreuva oil contains about farnesol of 2 to 3% and about nerolidol of 75 to 80%.Before using, with 100 to 300 times of the prepared product dilute with waters of embodiment 5.

U.S. Pat 4,775,534 disclose a kind of miticide composition that is suitable for foliar spray control tetranychid population.The preparation of above-mentioned miticide composition is to flood sustained release matrix to form suspension concentrates or wetting powder by usage alcohol and/or nerolidol.Sustained release matrix is regulated the carrier of material as slow release behavior after acaricide composition is administered to the blade face.Farnesol and nerolidol can improve male tetranychid natural, instinct motor behavior and search active.The random motion probability increases as a result, has improved the possibility that above-mentioned male tetranychid and miticide in being combined in miticide composition directly contact and has improved acaricidal effectiveness.The final concentration of farnesol in final Spray Mixing thing should be at least 15ppm, but it is said that concentration surpasses the effectiveness that 200ppm can not improve miticide composition.The concentration of nerolidol in final Spray Mixing thing is at least 10ppm, but the concentration of nerolidol is 100ppm or when surpassing 100ppm, the result does not show can obtain any beneficial effect in final Spray Mixing thing.In final Spray Mixing thing, preferred nerolidol content range is 5ppm to 50ppm.At least comprise 100mg farnesol and 50mg nerolidol in the preferred miticide composition preparation.

About the application of farnesol and/or nerolidol, prior art mainly is conceived to male tetranychid.As implied above, U.S. Pat 4,775,534th directly causes male tetranychid behavior change.And at Environmental Entomology, 4 volumes are primarily aimed at the male attractant of farnesol as two-spotted spider mite in the early stage work that 2 phases delivered at Regev and Cone.In above-mentioned paper, observe the farnesol male tetranychid (seeing 310 pages) that when concentration is 200ppm and Geng Gao, can cause death.In Regev and Cone one piece of paper (Environmental Entomology, 5 volumes, 1 phase) subsequently, mention nerolidol, rather than geraniol, be the sex attractant of a kind of male tetranychid.The nerolidol of finding 100ppm can not attract male tetranychid.(seeing 137 pages).

But as previously described, nerolidol and farnesol are the sex attractants of male tetranychid, and male tetranychid provides enough seminal fluid to female tetranychid in insemination first time process, make its ovum breed the mite of two kinds of sexes, up to female mite death.Regev and Cone are explained as follows: if parent is fertilized, can breed the tetranychid of two kinds of sexes from these ovum; Therefore, a kind of air borne type sex attractant must be arranged, it is not far that mating person is separated by each other.In addition, tetranychid is not genuine insect, and there is parthenogenesis in it, and promptly female tetranychid does not need at first to carry out mating with male tetranychid can breed male tetranychid.In the later research of Regev and Cone (Environmental Entomology, 5 volumes, 3 phases), they find to give birth to more ovum with the female mite that the female two-spotted spider mite of the solution Local treatment of 200ppm farnesol in 40% ethanol is only compared with 40% Ethanol Treatment.They infer that the female mite number of eggs laid of handling with farnesol increases the short sexual gland effect owing to farnesol.Although the farnesol of 200ppm is for example understood in this paper front male tetranychid is caused death, it is nontoxic to female tetranychid.By from female tetranychid, separating these chemical substances, identify the sex attractant that farnesol and nerolidol are male tetranychid in this respect.

Although U.S. Pat 4,775,534 disclosed materials can be lured male tetranychid on the miticide effectively, but this is the part of problem, the toxicity action of miticide need be improved at this, the problem that tetranychid is invaded and harassed can be more effectively solved like this female tetranychid of responsive and resistant population.

Consider the obvious different of the mode of action of mating pattern, sex attractant between tetranychid and the high insect form and daily behavior, it is that be not easy to find from the surface and unpredictable to the ability of the toxicity action of female tetranychid that the chemical substance miticide is regulated in male tetranychid behavior.

The present invention's general introduction

Therefore, the present invention relates to be suitable for being sprayed on the blade face preventing and treating the miticide composition of female and male tetranychid population, described miticide composition comprises and is selected from following miticide; Organotin, preferred fenbutatin oxide; Organophosphorus ester, preferred zolone; Imines (diamides), preferred amitraz; Sulfite, preferred propargite; Macrolide, preferred Olivomitecidin; And elementary sulfur; And the compound that is selected from sesquiterpene alcohols, its analog and composition thereof that improves the effective dose of selected acaricidal toxicity action, preferred farnesol, nerolidol and composition thereof.The sesquiterpene alcohols of effective dose and analog thereof the concentration range in final spray liquid is about 300ppm to about 1000ppm, and whether its concentration is responsive or have resistance and change according to the miticide that is used and target tetranychid population.

Therefore, the purpose of this invention is to provide a kind of miticide composition, described composition comprises a kind of miticide, and compares with independent application miticide, and said composition is to sensitivity and resistance is female and the control efficiency of male tetranychid obviously improves.

Another object of the present invention provides a kind of miticide composition, and said composition can make conventional acaricidal rate of application be lower than at present under situation about not reducing the level of preventing and treating of female and male tetranychid population.

Another object of the present invention provides the composition of forms such as the solution that is suitable for mixing with conventional miticide, emulsion agent, dispersant, pulvis, dust agent, granule, pill, so that easily prepare improved miticide composition.

A further object of the invention provides a kind of by using the method that all existence such as above-mentioned miticide composition control farmland, trees, greenhouse, garden and room are subject to the female and male tetranychid population on the blade face at plant place of tetranychid infringement.

To those skilled in the art, by following explanation, above-mentioned purpose with other of the present invention will become more obvious.

Explanation of the present invention

The present invention relates to be suitable for being sprayed on the blade face preventing and treating the miticide composition of female and male tetranychid population, this miticide composition comprises and is selected from following miticide; Organotin, preferred fenbutatin oxide; Organophosphorus ester, preferred zolone; Imines, preferred amitraz; Sulfite, preferred propargite; Macrolide, preferred Olivomitecidin; And elementary sulfur; And improve selected acaricidal toxicity action effective dose be selected from following sesquiterpenoid: sesquiterpene alcohols, its analog and composition thereof, the preferred farnesol of described compound, nerolidol and composition thereof.The sesquiterpenoid of effective dose and analog thereof the concentration range in final spray liquid is about 300ppm to about 1000ppm, and its concentration is according to the miticide that is used and target tetranychid population sensitivity or have resistance and change whether.

Although do not want to be subjected to the constraint of any particular theory, it is generally acknowledged that (for example the effect of farnesol and nerolidol is a physiological action to sesquiterpenoid.Think that specifically when female and/or male mite contacted with miticide composition, they obtained the miticide of virulence level separately.Usually, the immune system of tetranychid attempt to resist and in and the effect of toxic agent.It is believed that farnesol and/or nerolidol adhere to or be combined on the acceptor of tetranychid and substantially make its existing " turning a blind eye to " maybe can not make suitable reaction or make toxic agent inactivation fully toxic agent.Therefore, the tetranychid immune system can not in and the effect of toxic agent.

Farnesol and nerolidol are sesquiterpene alcoholses.Therefore, described chemical substance can be other sesquiterpene alcohols and analog thereof.

The pure and mild acaricidal composition of sesquiterpene provides the wonderful LC that reduces responsive and resistance tetranychid ₅₀The synergy of level.When sesquiterpene alcohols and the combination of specific insecticide, observe the Synergistic effect outside the similar expectation.Some miticides and insecticide comprise fenbutatin oxide, elementary sulfur, propargite, amitraz, zolone and Olivomitecidin.

In miticide composition, for the lethality that obtains obviously to improve, the sesquiterpene alcohols of effective dose or the concentration of its analog are more than or equal to 300ppm, but except the elementary sulfur, wherein the concentration of the sesquiterpene alcohols of effective dose and analog thereof is more than or equal to 500ppm.

Sesquiterpenoid can not only improve the activity level of specific miticide to responsive tetranychid, and can prevent and treat the tetranychid that above-mentioned miticide is had obvious resistance level.The present invention mainly is based on sesquiterpenoid and adheres to or be incorporated on the acceptor of tetranychid, has therefore hindered the identification of tetranychid to toxic agent.Therefore, it seems that shown effectiveness be physiological action, rather than the behavior effect.

Reactive compound.Be that miticide or insecticide and sesquiterpenoid can mix with the carrier of routine with such as surfactant, adhesive and stabilizing agent one analog assistant: according to a conventional method mixture is mixed with water-dispersible powder, colloidal sol (suspending agent) pulvis, DL (do not have and float) pulvis or granule then.

For water-dispersible powder, emulsion agent, liquor, glue, pulvis, DL pulvis and granule, the content of active component is about 1 to about 95% (weight) in the miticide composition of the present invention.Whether the mixing ratio of miticide and sesquiterpenoid is responsive or have resistance and determine according to the miticide that is used and target tetranychid.Table 1 shows this mixing ratio (weight: example weight).

Table 1

Reactive compound	The resistance tetranychid	Weight ratio 1
				Preferably	More preferably
		Fenbutatin oxide 2	Not			100∶1-1∶1	?10∶1-3∶1
Fenbutatin oxide 2	Be			5∶1-1∶4	?3∶1-1∶3
		Olivomitecidin 3	Not			50000∶1-5000∶1	?25000∶1-15000∶1
Amitraz 4	Not			100∶1-10∶1	?50∶1-10∶1
		Sulphur 5	Not			1∶200-1∶30	?1∶100-1∶60
Zolone 6	Be			2∶1-1∶14	?1∶1-1∶10
		Propargite 7	Not			100∶1-4∶1	?50∶1-10∶1

1. weight ratio is the weight ratio of FCI-119 and reactive compound, wherein FCI-119 be farnesol and nerolidol mixture (by weight, farnesol (52.5): nerolidol (42.5): surfactant (5.0)), buy from Fermone Corporation.

2.Vendex?50WP。

3.Avid?0.15EC。

4.Amitraz?1.5EC。

5.Thiolux?80％a.i.

6.Zolone?EC。

7.Omite?30W。

In table 1, fenbutatin oxide explanation reactive compound/sesquiterpene composition has indicated the effectiveness of above-mentioned composition antagonism population to the effect of the female tetranychid of sensitivity.Therefore, the toxicity action of expectation Olivomitecidin, amitraz, sulphur and propargite antagonism strain can be by improving the combination of they and sesquiterpene alcohols (for example, farnesol, nerolidol, its analog and composition thereof).For zolone, add the toxicity action that sesquiterpene alcohols can not improve zolone, this shows the sensitive strain that supplies examination really to the zolone sensitivity, and the adding mentioned component can not improve its effectiveness.

Miticide composition of the present invention can be forms such as suspension concentrates, solution, emulsion agent, dispersant, powder agent, pulvis, granule, pill.Those skilled in the art are very familiar to the method for the agriculturally useful compositions of preparing above-mentioned form usually.The conventional method for preparing the composition of forms such as above-mentioned solution, emulsion agent, dispersant, powder agent, pulvis, granule, pill can be at Chemicals For Crop Protection And Pest Control, Green, Hartley and West work, find among the Pergamon Press Oxford 1977, the document is hereby incorporated by.More detailed data about the preparation of pulvis, wettable powder agent and granule can find in the technical papers that is entitled as " Formulation of Pesticidal Dusts; Wettable Powders and Granules ", the author is J.A.Polon, publication is on Pesticide Formulations, W.Van Valkenberg edits, and Marcel Dekker publishes, New York.N.Y.1973, the 143-234 page or leaf, the document is incorporated herein by reference.Further data about the preparation agricultural chemicals suspension agent can find in " Flowable Pesticide Formulations:Development; Processand the Need for Standard Testing Procedures ", the author is C.G.Halliday, publication is on Pesticide Formulations andApplication Systems, K.G.Seymour edits, American Societyof Testing Materials publishes, STP795,1983, the 45-52 page or leaf, the document is incorporated herein by reference.

Can be randomly auxiliary agent, thinner, modifier or the conditioner of the pest control of routine be joined in the preparation, in for example above-mentioned suspension concentrates.The example of above-mentioned auxiliary agent comprises defoamer (as dimethyl polysiloxane), pH buffer (as dimethyl polysiloxane), pH buffer (as the poly-ethoxy acetaldehyde of alkylaryl) and compatilizer (as pure sulfuric ester).Above-mentioned auxiliary agent can be from KaloAG Chem, Inc.of Overland Park, and Kans buys.The example of required thinner is to disperse sticker, as from Buckner, and the alkylaryl polyoxyethylene glucose that the Rigo company of Ky buys.

Spendable carrier can be any solid or the liquid that is used for agricultural chemicals in this miticide composition.

The example of solid carrier comprises mineral powder (as kaolin, bentonite, clay, imvite, talcum powder, diatomite, mica, quartz sand, ammonium sulfate and urea), plant powder (as bean powder, flour, wood chip, tobacco powder, starch and avicel cellulose) aluminium oxide, silicate, high dispersive silicate and wax.

The example of liquid-carrier comprises water, alcohols (as methyl alcohol, ethanol, normal propyl alcohol, isopropyl alcohol, n-butanol, ethylene glycol and benzylalcohol); Arene (benzene,toluene,xylene, ethylbenzene, chlorobenzene, isopropylbenzene and methyl naphthalene); Halogenated hydrocarbon (chloroform, carbon tetrachloride, carrene, vinyl chloride, Arcton 11 and dicholorodifluoromethane); Ethers (ether, oxirane, diox and oxolane); Ketone (acetone, MEK, cyclohexanone and methyl iso-butyl ketone (MIBK)): ester class (ethyl acetate, butyl acetate, ethylene glycol ethyl ethers acid esters and aluminium oxide acetic acid esters); Nitrile (acetonitrile, propionitrile and acrylonitrile); Sulfoxide class (methyl-sulfoxide); Alcohol ethers (glycol monoethyl ether and ethylene glycol monoethyl ether); Aliphatic series or cycloaliphatic hydrocarbons (n-hexane and cyclohexane); Industrial gasoline (benzinum and solvent naphtha); And petroleum distillate (paraffin, kerosene and light oil).

Other conventional miticide and insecticide also can be sneaked in the above-mentioned miticide composition as those that sell with trade mark " KELTHANE ", " CARZOL " and " CAPTURE ".Although it seems that at present the effectiveness to tetranychid did not strengthen when above-mentioned miticide and insecticide mixed with nerolidol and/or farnesol, their participants can strengthen its effect and therefore weaken the resistance of above-mentioned tetranychid during because of the insecticide that mixes synergy with nerolidol and/or farnesol and miticide combination.

Embodiment material and method

The miticide sensitive strain of two-spotted spider mite (Tetranychus urticaekoch (TSSM)) and various resistant strain are used for testing various mites and the Pesticidal compound effects with sesquiterpene alcohols farnesol and nerolidol, its analog and composition thereof combination time of killing.Responsive and resistant strain is by Plant Science, Inc., and Watsonville, CA (PSI) provides.Before about 30 years, PSI has set up above-mentioned sensitive strain at first.Resistant strain is PSI near Watsonville, separates in the commercial strawberry farm of CA.PSI carries out conventional screening to keep genetics of resistance and to cultivate to all resistant strains in the growth room under the environmental condition that helps the maximum growth amount.Population is raised on the flat piece of 14 " * 19 " Henderson bush bean (little lima bean): changed flat piece one time in every 7-10 days, so that suitable nutritional need to be provided.The growth room remains on 23-25 ℃ (73 ± 3 °F), and 24/0 hour illumination/dark cycle is promptly under continuous light.

Measure independent miticide, independent sesquiterpene alcohols or the LC of its composition with following bioassay method ₅₀, LC ₉₅And/or per cent death loss value.Before processing about 24 hours, respectively with sensitivity or resistant strain about 12 to about 20 female one-tenth mites or sex mix if tetranychid moves on to from the blade that is infected on the Kidney bean blade of 15mm diameter of cutting-out, this Kidney bean blade be placed in 1 ounce of plastic cup (" biologicall test ring ") with on the gentle cotton balls of deionized water.Under room temperature (being 23-25 ℃) condition, tetranychid is remained in the growth room round the clock of 16/8 illumination/dark cycle (illumination in promptly 16 hours and 8 hours dark), make it adapt to the weather (promptly beginning to get food, knot and lay eggs) on ground, the new home.Each biologicall test comprises that four repetition blades of each dosage add a untreated control blade.With compound name, handle number and each repeated number that repeats to each biologicall test cup separate marking (with the black ink that is difficult for wiping).

On the same day of dispenser (just before spraying), remove all dead or small and weak tetranychids, and count and write down the number (using binocular microscope) of healthy mite on each blade exactly.Prepare miticide (at its predetermined LC from mother liquor ₅₀The various spray solutions of 100mL are provided respectively dosage) and a series of dilutions of the composition of sesquiterpene alcohols (at various dosage).The solution of each dosage is put into the 125mL conical flask of the band stirrer that has its unique tag.Before dispenser and in the dispenser process,, mix the solution of various dosage continuously by solution being placed on the magnetic agitation dish.Use is connected to the spraying apparatus on the ring that is fixed on about 15cm place, target surface, handles each blade and tetranychid of repeating with the dosage solution (at 20psi) of test compound.Distance between adjusting vane and the shower nozzle makes just can provide the covering fully to target leaf by spray pattern.Each is repeated about 1/2 second of sample (blade) spraying, adopt the rheostat timing device timing of solenoid starting.

After the processing, each blade is moved in the plexiglass-room (the Munger cell of transformation) that a complete beans leaf (on the pad of cotion of humidity) is housed immediately, above-mentioned beans leaf is handled and the dosage spraying with same in advance.After spraying is handled but before the processed blade of handover, the middle level of cell (contain 1 " cell of diameter is placed processed blade/tetranychid therein) is put on blade and the pad of cotion.Blade after will handling then move into cell and the 3rd layer of lucite (have with the nylon wire covering 1 " the diameter aperture) be placed on the top of cell subsequently, have rubber strip that the cell of whole " sandwich " shape is held together.With suitable compounds name and processing and repeated number each cell of mark individually.Cell is placed on 23-25 ℃ (73 ± 3 °F), and following 6 days of illumination in 16/8 hour/dark cycle condition is estimated the lethality of tetranychid therebetween.

The lethality of TSSM when estimating after the dispenser (PAI) 2 days (48 hours), 4 days (96 hours) and/or 6 days (144 hours) at interval.In each the evaluation, counting and record survive, the dead and tetranychid number of escaping.Go out the per cent death loss (proofreading and correct) of conversion with the tetranychid number of escaping from these data computation.For LC ₅₀And LC ₉₅Test is added up the lethal concentration of determining test compound by the equation of linear regression that is calculated by calculator both rate routine analyzer subsequently.Then these data are carried out binary ANOVA statistical analysis, to determine whether to exist between each processing the difference on the statistics.Data are expressed as the average mortality of (being generally form) each processing or handle and corresponding tetranychid type for each, promptly sensitivity or resistance with become mite or young mite kill 50% (LC ₅₀) and 95% (LC ₉₅) confession plant experimentally group needed active component ppm concentration.

Test compound is:

CAPTURE 2EC (missible oil) (Capture)

-common name: bifenthrin (CAS82657-04-3)

-chemical name: 3-(2-chloro-3,3,3-three fluoro-1-acrylic-2,2-

Dimethyl cyclopropane carboxylic acid 1 α, 3 α (Z)]-(±)-(the 2-methyl [1,

1 '-xenyl]-the 3-yl) methyl esters

-synthetic pyrethroid insecticide/miticide

-FMC Corp. production and selling.

ZOLONE EC (missible oil) (Zolone)

-common name: zolone (CAS 2310-17-0)

-chemical name: S-[(6-chloro-2-oxo-3 (2H)-benzoxazoles

Base) methyl] O, the O-diethyl phosphorothioate

-organophosphorus compounds miticide, insecticide

-Rhone Poulenc production/sale

DICOFOL 4EC (missible oil) (Dicofol)

-common name: dicofol (CAS115-32-2)

-chemical name: 1, two (chlorphenyl)-2,2 of 1-, 2-ethapon

-chlorinated hydrocarbon miticide

-Rohm ﹠amp; Hass (claims kelthane) ﹠amp again; Mahkteshim-Agan production and selling CARZOL 2EC (missible oil) (Carzol)

-common name: volt kills mite hydrochloride (CAS 23422-53-9)

-chemical name: N, N-dimethyl-N '-[the 3-[[(methylamino) carbonyl] the oxygen base] phenyl] the methylene imine mono-hydrochloric salts

-carbamate insecticide/miticide

-NOR-AM chemical company production and selling.OMITE 30W (wetting powder) (Omite)

-common name: propargite (CAS2312-35-8)

-chemical name: 2-[4-(1, the 1-dimethyl ethyl) phenoxy group] cyclohexyl-2-propynyl sulphite

-sulfurous esters miticide

Production/the sale of-Uniroyal chemical company.AMITRAZ 1.5EC (missible oil) (Amitraz)

-common name: amitraz (CAS 33089-61-1)

-chemical name: N '-(2, the 4-3,5-dimethylphenyl)-N-[[(2, the 4-3,5-dimethylphenyl) imino group] methyl]-the amino amine of N-methyl methylene

-imines insecticide/miticide

-NOR-AM chemical company sells.THIOLUX80%a.i. (sulphur suspended particulates) (Thiolux)

—(CAS7704-34-9)

-miticide/fungicide

-Sandoz Ltd., Sandoz Crop Protection Corp. (U.S.) sells.AVID 0.15 EC (a/k/a Abamectin 0.15EC) (missible oil) (Avid)

-common name: Olivomitecidin (a kind of macrolide)

-chemical name: Olivomitecidin B ₁: the minimum 80% Olivomitecidin B that contains ₁A (5-0-demethylation Olivomitecidin A ₁A) and at most contain 20% Olivomitecidin B ₁(5-0-demethylation-25-takes off-1-methyl-propyl-25-(1-Methylethyl) Olivomitecidin A b ₁A) mixture

-the natural products that produces by streptomycete (Streptomyces avermitilis): insecticide/miticide

-Merck ﹠amp; Co., Inc. production/sale.VENDEX 500 WP-(wetting powder) (Vendex)

-common name: fenbutatin oxide (CAS-13356-08-6)

-chemical name: two [three (2-methyl-2-phenyl propyl) tin] oxide (six (2-methyl-2-phenyl propyl) distannoxane)

-organic tin miticide

-DuPont Agricultural Products production/sale.Sesquiterpene alcohols and analog thereof for examination are: FCI-119a (K): farnesol (sesquiterpene alcohols)

—(CAS4602-84-0)

-chemical name: 3,7,11-trimethyl-2,6,10-12 carbon triolefin-1-alcohol

-from Fermone Corporation, Phoenix, AZ buys.

-FCI-119b (G): nerolidol (sesquiterpene alcohols)

—(CAS7212-44-4)

-chemical name: 3,7,11-trimethyl-1,6,10-12 carbon triolefin-3-alcohol

-from Fermone Corporation, Phoenix, AZ buys.

FCI-119: farnesol: flores aurantii power alcohol: anionic/nonionic surfactant

(Armul33) (weight ratio 52.5: 42.5: 5.0)

-from Fermone Corporation, Phoenix, AZ buys.

FME: farnesyl-methyl ether (farnesol analog).

Embodiment 1:FCI-119 at several miticides to the effect on the activity of two-spotted spider mite (Tetranychusurticae Koch)

Measured the effect of FCI-119 in the present embodiment to several miticide activity.Miticide Capture, Zolone, Kelthane have been estimated ^TM(Dicofol) and the activity of the two-spotted spider mite of Carzol antagonism strain (Tetranychus urticae Koch (TSSM)).In addition, can improve the effectiveness of these compounds, then fasten, the activity when measuring these compounds and FCI-119 combination at TSSM product to the miticide sensitivity if find FCI-119.Activity when also on to the TSSM strain (not obtaining resistant strain) of miticide sensitivity, measuring Omite 30W, AmitrazM 1.5 EC, Thiolux 80W and Avid 0.15 EC and FCI-119 combination.These tests are all carried out with the FCI-119 of 1000ppm.Thiolux and 500ppm FCI-119 on sensitive strain TSSM, have also been measured and the activity when making up with the 1000ppm nerolidol.

The biologicall test of using Capture, Carzol and Kelthane (Dicofol) to carry out on pyrethroid, carbamate and chlorohydrocarbon resistant strain respectively shows, the composition of they and 1000ppmFCI-119 does not demonstrate active obvious raising statistically (that is, for LC ₅₀).Therefore, on the sensitive strain of TSSM, do not carry out biologicall test with these compounds.Table 4-6 has shown the data of these tests 4 days or 6 days with the form of tabulation.

The organophosphorus ester resistance two-spotted spider mite female one-tenth mite of table 2:FCI-119 to handling with Zolone

At the LC of dispenser after 4 days and 6 days ₅₀Value and LC ₉₅The influence of value

Processing+FCI-119 (@.ppm)	4 days (ppm)		6 days (ppm)
					????LC 50	?????LC 95	????LC 50	????LC 95
	Independent Zolone a	????5690	????14560	????--					????--
Independent Zolone b					????4452	????10258	????3179	????13743
	?Zolone+FCI-119@1000ppm	????731	????6873	????--					????--
?Zolone+FCI-119@1000ppm b					????780	????4310	????498	????1459

A: back-to-back test b: back-to-back test

Table 3:FCI-119 exists to the female one-tenth mite of responsive two-spotted spider mite of handling with Zolone

The LC of dispenser after 4 days and 6 days ₅₀Value and LC ₉₅The influence of value

Processing+FCI-119 (@ppm)	4 days (ppm)		6 days (ppm)
					????LC 50	???LC 95	??LC 50	???LC 95
	Independent Zolone	?????9	????34	????9					????32
Zolone+FCI-119@1000ppm					????11	????50	????7	????54

The carbamate resistance two-spotted spider mite of table 4:FCI-119 to handling with Zolone

Female one-tenth mite is at the LC of dispenser after 4 days ₅₀Value and LC ₉₅The influence of value

Processing+FCI-119 (@ppm)	4 days (ppm)
			????LC 50	????LC 95
	Independent Carzol	????1461			????7526
Carzol+FCI-119@1000ppm			????2186	????6367

The pyrethroid resistance two-spotted spider mite of table 5:FCI-119 to handling with Capture

Female one-tenth mite is at the LC of dispenser after 6 days ₅₀Value and LC ₉₅The influence of value

Processing+FCI-119 (@ppm)	6 days (ppm)
			????LC 50	????LC 95
	Independent Capture	????164			????1478
Capmre+FCI-119@1000ppm			????173	????1576

Table 6:FCI-119 is female to the chlorohydrocarbon resistance two-spotted spider mite of handling with Dicofol

Become mite at the LC of dispenser after 6 days ₅₀Value and LC ₉₅The influence of value

Processing+FCI-119 (@ppm)	6 days (ppm)
			????LC 50	????LC 95
	Independent Dicofol	????1281			????6229
?Dicofol+FCI-119@1000ppm			????1304	????9460

The responsive two-spotted spider mite female one-tenth mite of table 7:FCI-119 to handling with Omite

At dispenser LC after 4 days and 6 days ₅₀Value and LC ₉₅The influence of value

Processing+FCI-119 (@ppm)	4 days (ppm)		6 days (ppm)
					???LC 50	???LC 95	????LC 50	????LC 95
	Independent Omite	????70	???228	????10					????171
?Omite+FCI-119@1000ppm					????20	????99	????10	????100

The responsive two-spotted spider mite female one-tenth mite of table 8:FCI-119 to handling with Amitraz

At the LC of dispenser after 4 days and 6 days ₅₀Value and LC ₉₅The influence of value

Processing+FCI-119 (@ppm)	4 days (ppm)		6 days (ppm)
					????LC 50	????LC 95	????LC 50	????LC 95
	Independent Amitraz	????102	????528	????37					????344
Amitraz+FCI-119@1000ppm					?????17	????123	????12	?????82

The responsive two-spotted spider mite female one-tenth mite of table 9:FCI-119 to handling with Abamectin

At the LC of dispenser after 4 days and 6 days ₅₀Value and LC ₉₅The influence of value

Processing+FCI-119 (@ppm)	4 days (ppm)		6 days (ppm)
					????LC 50	???LC 95	????LC 50	????LC 95
	Independent Abamectin	????0.07	????0.2	????0.03					????0.08
Abamectin+FCI-119@500ppm					????0.01	????0.04	????0.008	????0.04

The responsive two-spotted spider mite female one-tenth mite of table 10:FCI-119 to handling with Thiolux

At the LC of dispenser after 4 days and 6 days ₅₀Value and LC ₉₅The influence of value

Processing+FCI-119 or nerolidol (@ppm)	4 days (ppm)		6 days (ppm)
					????LC 50	???LC 95	????LC 50	?????LC 95
	Independent Thiolnx	???57392	?824430	???17513					???513015
Thiolux+FCI-119@1000ppm					????1348	??15259	????64.4	?????7649
	Thiolux+FCI-119@1000ppm	????1649	??87113	?????204					????15949
Thiolux+FCI-119@500ppm					????10168	?561266	????1727	????54141
	Thiolux+ Cheng Huashuchun @1000ppm	????18134	?205080	????3541					????71951

As show as shown in the 4-6, on carbamate (for example Carzol), chlorinated hydrocabon (for example Dicofol) or pyrethroid (for example Capture) the miticide resistant strain of TSSM, the FCI-119 of 1000ppm does not improve the effectiveness of above-claimed cpd.

On the organophosphorus ester resistant strain of TSSM, the FCI of 1000ppm can improve the effectiveness of Zolone3EC.(" 3EC " equals 3 pounds of Zolone in the per gallon missible oil) (seeing Table 2).Fasten at responsive TSSM product, FCI-119 it seems the effectiveness (seeing Table 3) that can not improve Zolone 3EC.This shows that above-mentioned tetranychid is really responsive to Zolone.

To the TSSM of sensitivity, after dispenser 4 days, the FCI of 1000pmm has certain synergistic effect to Omite 30W, but at the 6th day, no longer includes synergistic effect., kill capability is useful faster; Promptly 4 days to 6 days (referring to table 7).The FCI-119 of 1000ppm has medium synergistic effect (seeing Table 8) to the responsive TSSM that handles with Amitraz 1.5EC.It seems that the FCI-119 of 500ppm the responsive TSSM that handles with Avid 0.15EC is demonstrated synergistic effect (seeing Table 9).

500 and the FCI-119 of 1000ppm as if strengthen the effectiveness of Thiolux to responsive TSSM.The nerolidol of 1000ppm also strengthens the effect (see Table 10) of Thiolux to responsive TSSM.

Embodiment 2:FCI-119 becomes mite and if the effect on the mite activity at Olivomitecidin (Avid) to responsive two-spotted spider mite (Tetranychus urticae Koch)

In this embodiment, the one-tenth mite of the miticide sensitive strain of two-spotted spider mite (Tetranychus urticae Koch) with if use the composition of the FCI-119 dilution of miticide Olivomitecidin and 8 kinds of serial dosage on the mite, measure the effect of FCI-119 to Olivomitecidin 0.15EC (a/k/a Avid 0.15EC) activity.Serial dilutions and known LC with eight kinds of FCI-119 ₅₀Olivomitecidin 0.15EC mix, then these dilutions are administered to female one-tenth mite of two-spotted spider mite (TSSM) and sex and mix on the bitter mite, whether can increase the lethality that exposes mite to measure FCI-119.

Handling the previous day, with the only responsive product female one-tenth mite of silk barathea two spotted spider mite of 12-15 or sex mix if mite moves on to from the blade that is infected on the Kidney bean blade of 15mm diameter of cutting-out, above-mentioned blade is placed on using on the moistening cotton balls of deionized water in " biologicall test cup ".

LC by independent test determination Olivomitecidin 0.15EC ₅₀Calculate the LC of responsive two-spotted spider mite one-tenth mite ₅₀Value is 0.016ppm.If two-spotted spider mite is the LC of mite ₅₀Value is 0.029ppm.Processing comprises that Olivomitecidin 0.15EC (uses its LC ₅₀Value) with 300,100,30,10,3,1,0.3 and the composition of 0.1ppm ECI-119, independent Olivomitecidin 0.15EC and untreated control.

At interval during (PAI) two days (48 hours) and four days (96 hours), the lethality evaluation is carried out in all tests after the dispenser.The biometric data of representing with tabular form is the average mortality (table 11 and 12) to become mite and handle as if mite at every turn under each FCI-119 concentration.

Biologicall test test to responsive silk cotton spider adult shows, when using LC ₅₀When the Olivomitecidin 0.15EC of concentration tested, the FCI-119 of any dosage of 0.1 to 300ppm did not all have tangible statistics synergistic effect to improving lethal., Olivomitecidin 0.15EC adds that the FCI-119 (300ppm) of maximum dose level can provide the mortality of greater number really.

To two-spotted spider mite if the biologicall test of mite shows that high concentration FCI-119 has certain synergistic effect to lethal.This effect is not obvious, because the statistics per cent death loss of 300ppm, 100ppm, 10ppm, 3ppm and 0.3ppm dosage equates.

Table 11. pair is used LC ₅₀The Olivomitecidin 0.15EC of value and the FCI-119's of various dosage

The responsive two-spotted spider mite that composition spray is handled becomes mite at 4 days post doses of dispenser

The biometric data of/per cent death loss ¹

Compound/FCI-119 dosage	Average per cent death loss
		Olivomitecidin 0.15EC/300PPM	????61.25a
Olivomitecidin 0.15EC/100PPM	????39.50a
		Olivomitecidin 0.15EC/30PPM	????42.50a
Olivomitecidin 0.15EC/10PPM	????35.50a
		Olivomitecidin 0.15EC/3PPM	????43.75a
Olivomitecidin 0.15EC/1PPM	????21.50a
		Olivomitecidin 0.15EC/.3PPM	????29.75a
Olivomitecidin 0.15EC/.1PPM	????40.75a
		Olivomitecidin 0.15EC (separately)	????21.25a
Untreated control	????17.50a

¹According to DNMRT (P=0.05), the identical letter representation no significant difference of mark behind the mean value.

Table 12. pair is used LC ₅₀The Olivomitecidin 0.15EC of value and the FCI-119's of various dosage

The responsive two-spotted spider mite that composition spray is handled if mite at 4 days post doses of dispenser

The biometric data of/per cent death loss ²

Compound/FCI-119 dosage	Average per cent death loss
		Olivomitecidin 0.15EC/300PPM	????49.75a
Olivomitecidin 0.15EC/100PPM	????51.50a
		Olivomitecidin 0.15EC/30PPM	????31.75bc
Olivomitecidin 0.15EC/10PPM	????36.25abc
		Olivomitecidin 0.15EC/3PPM	????42.50ab
Olivomitecidin 0.15EC/1PPM	????33.75bc
		Olivomitecidin 0.15EC/.3PPM	????42.50ab
Olivomitecidin 0.15EC/.1PPM	????26.75c
		Olivomitecidin 0.15EC (separately)	????21.50cd
Untreated control	????12.00d

²According to DNMRT (P=0.05), the identical letter representation no significant difference of mark behind the mean value.

Embodiment 3: the FCI-119 that increases dosage becomes mite and if the effect on the mite activity at Olivomitecidin (Avid) to responsive two-spotted spider mite (Tetranychus urticae Koch)

In this embodiment, the one-tenth mite of the miticide sensitive strain of two-spotted spider mite (Tetranychus urticae koch) with if use the composition of the FCI-119 dilution of miticide Olivomitecidin and 6 kinds of serial dosage on the mite, measure the effect of FCI-119 to Olivomitecidin 0.15EC (a/k/a Avid 0.15EC) activity.Present embodiment is the continuity of embodiment 2, and embodiment 2 has tested the FCI-119/ Olivomitecidin composition of low concentration.The FCI-119 that has determined higher concentration in embodiment 2 may increase activity.Six serial dilutions and known LC with FCI-119 ₅₀The Olivomitecidin 0.15EC of value mixes.Then these dilutions are administered to that female one-tenth mite of two-spotted spider mite (TSSM) and sex are mixed if on the mite, whether can increase the lethality that exposes mite to measure FCI-119.

Test determination the LC of composition of Olivomitecidin 0.15EC and Olivomitecidin/FCI-119 ₅₀Value.In above-mentioned bioassay method, to each repetition, the previous day of handling, with 12-15 the female one-tenth mite of sensitive strain two-spotted spider mite or sex mix if mite moves on to from the blade that is infected on the Kidney bean blade of 15mm diameter of cutting-out, above-mentioned blade be placed in " biologicall test cup " with on the moistening cotton balls of deionized water.

As described in example 2 above, with the LC of independent biologicall test test determination Olivomitecidin 0.15EC ₅₀Value.For this test, the sensitive strain two-spotted spider mite is become mite in decision and if mite is used the calculating LC of the upper limit (being fiducial limit) that approaches benchmark ₅₀Value.To the LC of responsive two-spotted spider mite as if the mite use ₅₀Value is the 0.038ppm Olivomitecidin, and to the use of responsive two-spotted spider mite one-tenth mite is 0.17ppm.Processing comprises Olivomitecidin 0.15EC (SC service ceiling benchmark LC ₅₀Value) with 3000,1000,300,100,30 and the composition of the FCI-119 of 10ppm, independent LC ₅₀The Olivomitecidin of concentration and untreated control.

At interval during (PAI) four days (96 hours) and six days (144 hours), the lethality evaluation is carried out in all tests after the dispenser.The biometric data of representing with tabular form is the average mortality (table 13 and 14) to become mite and handle as if mite at every turn under each FCI-119 concentration.

Become the bioassay results of mite to show to two-spotted spider mite, dosage be 1000,300 and the FCI-119 of 100ppm lethal is had certain synergistic effect.The mutually the same statistically and lethality of these results is significantly greater than all other processing.The Olivomitecidin of maximum dose and 3000ppmFCI-119 handle the low dosage processing (30ppm and 10ppm) and the Olivomitecidin that equal all statistically and handle separately.3000ppm dosage become mite to lack significantly active to sensitivity and under above-mentioned dosage (3000ppm) to responsive two-spotted spider mite if the lower LC of mite ₅₀Numerical value shows, may suppress active under above-mentioned high dose.Untreated control has the lethality (table 13) that is starkly lower than all other processing in statistics.

To responsive two-spotted spider mite if the bioassay results of mite with become the biologicall test of mite closely similar to responsive two-spotted spider mite.3000,1000,300 and 100ppm dosage than low dosage, the independent statistics lethality height of Olivomitecidin and untreated control.3000ppm dosage is lower than the lethality of 1000ppm and 300ppm dosage, and identical with 10ppm dosage with 30ppm from statistics.Untreated control has the lethality (table 14) that is starkly lower than all other processing statistically.

FCI-119 provides obvious synergistic effect on the statistics really when being used in combination with Olivomitecidin.The highest lethality is at 55.5%/1000ppmm in the test of responsive two-spotted spider mite one-tenth mite, and the highest lethality is 74.5%/300ppm in responsive two-spotted spider mite is tested as if the biologicall test of mite.

Table 13. pair is used LC ₅₀The Olivomitecidin 0.15EC of value becomes the biometric data of mite in 6 days post dose/per cent death losses of dispenser with the responsive two-spotted spider mite that the composition spray of the FCI-119 of various dosage is handled ³

Compound dosage	Average per cent death loss (6 days)
		Olivomitecidin+FCI-119@3000ppm	????37.3b
Olivomitecidin+FCI-119@1000ppm	????55.5a
		Olivomitecidin+FCI-119@300ppm	????52.5a
Olivomitecidin+FCI-119@100ppm	????53.8a
		Olivomitecidin+FCI-119@30ppm	????38.8b
Olivomitecidin+FCI-119@10ppm	????26.5b
		Olivomitecidin (separately)	????27.3b
Untreated control	????10.3c

³According to DNMRT (P=0.05), the identical letter representation no significant difference of mark behind the mean value.

Table 14. pair is used LC ₅₀The responsive two-spotted spider mite that the composition spray of the Olivomitecidin 0.15EC of value and the FCI-119 of various dosage is handled is as if the biometric data of mite in 6 days post dose/per cent death losses of dispenser ⁴

Compound dosage	Average per cent death loss (6 days)
		Olivomitecidin+FCI-119@3000ppm	????61.5ab????
Olivomitecidin+FCI-119@1000ppm	????74.3a???????
		Olivomitecidin+FCI-119@300ppm	????74.5a?????????
Olivomitecidin+FCI-119@100ppm	????61.5ab??????????
		Olivomitecidin+FCI-119@30ppm	????55.8b???????????
??	Olivomitecidin+FCI-119@10ppm
		????47.8bc??????????	Olivomitecidin (separately)
????39.0c??????????	Untreated control

⁴According to DNMRT (P=0.05), the identical letter representation no significant difference of mark behind the mean value.

Embodiment 4.FCI-119 becomes mite to go up active influence to Olivomitecidin (Ayid) responsive two-spotted spider mite (Tetranychus urticae Koch)

In the present embodiment, (TSSM) measured the LC of FCI-119 on the sensitive strain two-spotted spider mite (T.urticae Koch) to Olivomitecidin 0.15 EC of prior mensuration ₅₀And LC ₉₅The influence of value.

In this bioassay method, to each repetition, handling the previous day, 12-15 the female one-tenth mite of sensitive strain two-spotted spider mite moved on to from the blade that is infected on the Kidney bean blade of 15mm diameter of cutting-out, above-mentioned blade is placed in " biologicall test cup " with on the moistening cotton balls of deionized water.

The FCI-119 mother liquor of application same amount and the Olivomitecidin 0.15EC of various dosage prepare the serial dilutions of FCI-119/ Olivomitecidin spray.At interval the lethality that the FCI-119/ Olivomitecidin is tested (PAI) is estimated in (PAI) 4 days (96 hours) after dispenser.

The dilution of FCI-119/ Olivomitecidin such as following: (all dilutions are by the FCI-119 preparation of 1000ppm) 0.000005,0.0000042,0.0000033,0.0000025,0.0000018,0.000001,0.0000003 active component percentage.The dosage of the Olivomitecidin that uses is 0.00001 and 0.000005,0.0000042,0.0000033,0.0000025,0.0000018,0.000001,0.0000005,0.0000003 active component separately.

Olivomitecidin/FCI-119 result of the test shows that the FCI-119 of 1000ppm does not almost have synergistic effect to the female one-tenth mite of responsive two-spotted spider mite of handling with Olivomitecidin.The LC of independent Olivomitecidin ₅₀And LC ₉₅Be respectively 0.016ppm and 0.065ppm.The LC of the FCI-119 of Olivomitecidin and 1000ppm ₅₀And LC ₉₅Value is respectively 0.28ppm and 0.119ppm.

Look back the numerical value of relevant Olivomitecidin among the embodiment 1-4, the concentration range of sesquiterpenoid (being farnesol and the nerolidol among the FCI-119) is that about 300ppm is to about 1000ppm.In embodiment 2 (table 11), obtain the maximum lethality numerical value of responsive tetranychid with the FCI-119 of 300ppm.In embodiment 3, and compare with 300ppm with 1000ppm, lower with the FCI-119 lethality value of 3000ppm.In embodiment 4, from the result of embodiment 3, with the LC that compares independent use Olivomitecidin with the composition of the FCI-119 of 1000ppm ₅₀And LC ₉₅The sesquiterpenoid concentration that shows 1000ppm is its upper limit, and the results change that expectation obtains when relating to the Olivomitecidin of this low concentration.In addition, the result that this scope is implemented example 1 (table 9) confirms, in embodiment 1 FCI-119 of Olivomitecidin and 500ppm in conjunction with the time LC ₅₀And LC ₉₅Comparing with independent Olivomitecidin all has improvement.Six days data (table 14) have confirmed that also if above-mentioned scope also is suitable for responsive two-spotted spider mite mite in embodiment 3.

The composition of embodiment 5:FCI-119 and fenbutatin oxide (Vendex) 50WP to responsive and organotin resistance two-spotted spider mite (Tetranychus urticae Koch) strain become mite and as if the effect of mite

In the present embodiment, when fenbutatin oxide 50WP to become that mite and sex are mixed with two-spotted spider mite female that the composition forms of the FCI-119 dilution of 8 kinds of serial dosage is applied to the two-spotted spider mite (Tetranychusurticae Koch) of miticide sensitive strain and organotin resistant strain when if mite goes up, measured the effect of FCI-119 to fenbutatin oxide 50WP activity.Employed two-spotted spider mite organotin resistant strain is provided by PSI, PSI in 1988 near Watsonville, this strain is separated on the commercial strawberry farm of CA.The serial dilutions of eight kinds of FCI-119 and the fenbutatin oxide 50WP LC that measures in advance ₅₀The fenbutatin oxide of value mixes, to becoming mite and measuring as if mite.Become mite and the mixing sex that then these dilutions are administered to the two-spotted spider mite strain of miticide sensitivity and organotin resistance go up with mensuration FCI-119 whether to improve fenbutatin oxide 50WP covers tetranychid to nothing lethal as if mite.

The previous day of handling, with the female one-tenth mite of the two-spotted spider mite of 12-15 sensitive strain or organotin resistant strain or mix sex if mite moves on to from the blade that is infected on the Kidney bean blade of diameter 15mm of cutting-out, above-mentioned blade is placed in " biologicall test cup " with on the moistening cotton balls of deionized water.

The fenbutatin oxide 50WP solution of application same amount and the FCI-119 of various dosage prepare the serial dilutions of fenbutatin oxide 50WP and FCI-119 spray composite.With independent biologicall test measure responsive and organotin resistant strain become mite and as if the LC of mite to fenbutatin oxide 50WP ₅₀Value.Calculate responsive two-spotted spider mite is become mite and if the LC of mite ₅₀Value be respectively 251 with 23.5ppm: simultaneously organotin resistant strain two-spotted spider mite is become mite and if the LC of mite ₅₀Be respectively 1162 and 324ppm.Become mite to responsive with the two-spotted spider mite of organotin resistant strain, with fenbutatin oxide 50WP (with its LC ₅₀Value is used) be used in combination with 3000,1000,300,100,30,10,3 and the FCI-119 of 1ppm, add fenbutatin oxide 50WP and handle separately, and untreated control.To the two-spotted spider mite of responsive and organotin resistant strain as if mite, with fenbutatin oxide 50WP (with its LC ₅₀Value is used) be used in combination with 3000,1000,300,100,30 and the FCI-119 of 10ppm.In addition, also with 3000 and 1000ppm FCI-119 handle separately to measure the effect of FCI-119 itself.Using LC if also comprise in the mite test ₅₀The fenbutatin oxide 50WP of value handles and untreated control.

Become the mite test to carry out the lethality evaluation with organotin resistance two-spotted spider mite to responsive when (PAI) 2 days (48 hours), 4 days (96 hours) were with 6 days (144 hours) at interval after the dispenser.Responsive and organotin resistance two-spotted spider mite tested as if mite when 4 days (96 hours) and 6 days (144 hours) at interval after the dispenser and carry out the lethality evaluation.Because temporary transient dormancy of very difficult definite decortication mite and the difference between the death are confirmed two-spotted spider mite if the lethality evaluation at interval (PAI) is inaccurate after (48 hours) dispensers in two days of mite.Biometric data is expressed as become mite and if the average per cent death loss that mite is handled at every turn with tabular form.

Bioassay results to the female one-tenth mite of two-spotted spider mite shows at three maximum dose levels, promptly 3000,1000 and during 300ppm dosage, and FCI-119 is to the deadly remarkable effect that all has on the statistics of responsive and organotin resistant strain.The lethality that the FCI-119 of these three maximum dose levels provides is higher than statistically with low dosage FCI-119 and LC ₅₀Lethality (these data are summarised in table 15 and 16) during the compositions-treated of the fenbutatin oxide 50WP of value.

Two-spotted spider mite to the organotin resistant strain shows the FCI-119 of three maximum dose levels and LC if mite carries out the result of biologicall test ₅₀The statistics lethality that composition provided of the fenbutatin oxide 50WP of value also is higher than the FCI-119 than low dosage, but high dose differs from one another statistically (table 17).

The result that the FCI-119 of two maximum dose levels (3000 with 1000ppm) and the composition of fenbutatin oxide 50WP obtain in the biologicall test to the responsive nymph of miticide is identical statistically and is higher than the per cent death loss of all other processing.3000 and independent effect and the untreated control of the FCI-119 of 1000ppm dosage do not have difference (table 17 and 18) statistically.Therefore, independent FCI-119 does not have effect.

Table 15. pair with the FCI-119 of various dosage with at LC ₅₀The responsive two-spotted spider mite of the insecticide that the composition spray of the fenbutatin oxide 50WP of value is handled becomes the biometric data of dosage/per cent death loss of mite ⁵

Compound dosage	Average per cent death loss
				2 days	4 days	6 days
	Fenbutatin oxide+FCI-119@3000ppm	?47.5a	?79.0a				?100.0a
Fenbutatin oxide+FCI-119@1000ppm				?35.5b	?81.3a	?100.0a
	Fenbutatin oxide+FCI-119@300ppm	?16.0c	?68.5a				?93.5a
Fenbutatin oxide+FCI-119@100ppm				?9.3cd	?31.8bc	?62.3bc
	Fenbutatin oxide+FCI-119@30ppm	?2.0d	?21.5bc				?65.3b
Fenbutatin oxide+FCI-119@10ppm				?6.0cd	?33.8b	?55.0bcd
	Fenbutatin oxide+FCI-119@3ppm	?3.3d	?15.5cd				?46.8de
Fenbutatin oxide+FCI-119@1ppm				?4.5cd	?29.3bc	?51.3cd
	Independent fenbutatin oxide	?5.3cd	?18.3bc				?37.5e
Untreated control				?0.0d	?1.8d	?7.3f

⁵After handling with ANOVA and DNMRT (P=0.05), the letter representation that mark is identical behind the mean value is indifference each other statistically.

Table 16. couple FCI-119 and LC with various dosage ₅₀The organotin resistance two-spotted spider mite that the composition spray of the fenbutatin oxide 50WP of value is handled becomes the biometric data of dosage/per cent death loss of mite ⁶

Compound dosage	Average per cent death loss
				2 days	4 days	6 days
	Fenbutatin oxide+FCI-119@3000ppm	????39.3a	?93.8a				?96.0a
Fenbutatin oxide+FCI-119@1000ppm				????32.8ab	?80.0a	?91.5a
	Fenbutatin oxide+FCI-119@300ppm	????16.8c	?59.8b				?87.0a
Fenbutatin oxide+FCI-119@100ppm				????21.0bc	?46.8bc	?65.5b
	Fenbutatin oxide+FCI-119@30ppm	????19.0c	?49.5bc				?60.5b
Fenbutatin oxide+FCI-119@10ppm				????13.0cd	?45.0bc	?58.8b
	Fenbutatin oxide+FCI-119@3ppm	????10.3cd	?32.3c				?66.8b
Fenbutatin oxide+FCI-119@1ppm				????10.5cd	?38.8c	?55.3b
	Independent fenbutatin oxide	????11.8cd	?38.3c				?53.8b
Untreated control				????1.8d	?8.8d	?8.8c

⁶After handling with ANOVA and DNMRT (P=0.05), the letter representation that mark is identical behind the mean value is indifference each other statistically.

Table 17. couple FCI-119 and LC with various dosage ₅₀If the organotin resistance two-spotted spider mite that the composition spray of the fenbutatin oxide 50WP of value is handled is the biometric data of dosage/per cent death loss of mite ⁷

Compound dosage	Average per cent death loss
			4 days	6 days
	Independent FCI-119@3000ppm	?25.8de			?29.3ef
Independent FCI-119@1000ppm			?27.5de	?29.5ef
	Fenbutatin oxide+FCI-119@300ppm	?67.8b			?80.0b
Fenbutatin oxide+FCI-119@1000ppm			?90.3a	?96.0a
	Fenbutatin oxide+FCI-119@300ppm	?58.5b			?66.0c
Fenbutatin oxide+FCI-119@100ppm			?43.0c	?46.5d
	Fenbutatin oxide+FCI-119@30ppm	?33.0cd			?37.0de
Fenbutatin oxide+FCI-119@10ppm			?20.0de	?26.0ef
	Independent fenbutatin oxide	?27.5de			?27.5ef
Untreated control			?15.5e	?15.8f

⁷After handling with ANOVA and DNMRT (P=0.05), the letter representation that mark is identical behind the mean value is indifference each other statistically.

Table 18. couple FCI-119 and LC with various dosage ₅₀If the responsive two-spotted spider mite that the composition spray of the fenbutatin oxide 50WP of value is handled is the biometric data of dosage/per cent death loss of mite ⁸

Compound dosage	Average per cent death loss
			4 days	6 days
	Independent FCI-119@3000ppm	????15.3de			????15.3d
Independent FCI-119@1000ppm			????9.0e	????10.8d
	Fenbutatin oxide+FCI-119@3000ppm	????39.7b			????59.0a
Fenbutatin oxide+FCI-119@1000ppm			????57.5a	????62.8a
	Fenbutatin oxide+FCI-119@300ppm	????25.0cd			????32.3bc
Fenbutatin oxide+FCI-119@100ppm			????28.3c	????36.3b
	Fenbutatin oxide+FCI-119@30ppm	????13.8de			????22.5bcd
Fenbutatin oxide+FCI-119@10ppm			????10.5e	????18.3cd
	Independent fenbutatin oxide	????12.8e			????19.5cd
Untreated control			????9.8e	????14.3d

⁸After handling with ANOVA and DNMRT (P=0.05), the letter representation that mark is identical behind the mean value is indifference each other statistically.

Embodiment 6: the ratio test on two-spotted spider mite (Tetranychus urticae Koch) provides the ratio of effective facilitation with the pure and mild nerolidol of determination method to fenbutatin oxide 50WP

In the present embodiment, use the farnesol of different proportion and nerolidol (the sesquiterpene alcohols composition of FCI-119) to carry out the ratio test to fenbutatin oxide 50WP.This experiment is carried out on the two-spotted spider mite of responsive and organotin resistant strain.Ratio test on responsive two-spotted spider mite is to adopt various processing and farnesol and nerolidol and LC ₅₀The composition of the fenbutatin oxide 50WP (200ppm) of value carries out.Handle organotin resistance tetranychid with the fenbutatin oxide 50WP of 1000ppm and the composition spray of farnesol nerolidol.The ratio of using following (farnesol is preceding, nerolidol after, by ppm): 1000/0,850/150,650/350,550/450,350/650,150/850,0/1000, comprise that also a fenbutatin oxide handles separately and a untreated control.Biometric data is expressed as the average per cent death loss of each processing with the form of tabulation.

Ratio test is the result show, the farnesol of all tests/nerolidol ratio can both improve the activity (table 19) of fenbutatin oxide 50WP.Farnesol/the nerolidol of all proportions is all much better with fenbutatin oxide 50WP than single.It seems that the ratio of 650/350 farnesol/nerolidol behaves oneself best., 150/850 mixture is also very effective.It seems that farnesol and nerolidol have activity much at one.

The farnesol of table 19. different proportion and nerolidol become the effect of the lethality of mite with the resistant strain two-spotted spider mite to the sensitivity of handling with fenbutatin oxide 50WP ⁹

Processing+farnesol nerolidol (ppm/ppm)	Average per cent death loss
					Responsive		The organotin resistance
	4 days	6 days	4 days	6 days
					1. fenbutatin oxide *+1000/0	?98.3a	?100.0a	?58.8c	?88.1c
2. fenbutatin oxide *+850/150	?86.3b	?100.0a	?61.9bc	?83.3c
					3. fenbutatin oxide *+650/350	?100.0a	?100.0a	?90.5a	?100a
4. fenbutatin oxide *+550/450	?100.0a	?100.0a	?59.5bc	?83.2c
					5. fenbutatin oxide *+350/650	?86.8b	?98.2a	?80.8a	?96.8ab
6. fenbutatin oxide *+150/850	?100.0a	?100.0a	?82.6a	?98.3ab
					7. fenbutatin oxide *+0/1000	?89.9b	?98.2a	?75.1ab	?98.2ab
8. independent fenbutatin oxide *	?39.3c	?60.7b	?26.8d	?46.6d
					9. untreated control	?13.4d	?23.5d	?9.8e	?20.9e

^*Responsive two-spotted spider mite is the 200ppm fenbutatin oxide

^*Organotin resistance two-spotted spider mite is the 1000ppm fenbutatin oxide

⁹According to DNMRT/P=0.05, indifference on the letter representation statistics that mark is identical behind the mean value.

Embodiment 7: when the two-spotted spider mite (Tetranychusurticae Koch) of organotin resistance and sensitive strain goes up farnesol and nerolidol and makes up with fenbutatin oxide WP to the LC of fenbutatin oxide ₅₀And LC ₉₅The influence of value

In the present embodiment, the two-spotted spider mite of having measured in organotin resistance and sensitive strain (Tetranychus urticae Koch) (TSSM) goes up farnesol and the nerolidol LC to the fenbutatin oxide 50WP of prior mensuration ₅₀And LC ₉₅The influence of value.Provide TSSM organotin resistant strain by PSI, PSI 1988 near Watsonville.CA, commercial strawberry farm in isolate this strain.

Present embodiment is continuing of embodiment 5, has confirmed among the embodiment 5 that use FCI-119 (composition of farnesol and nerolidol) can reduce the LC of fenbutatin oxide 50WP to two kinds of two-spotted spider mite strains significantly ₅₀And LC ₉₅Value.In the present embodiment, the component of having tested FCI-119 is respectively reducing LC to measure each component ₅₀Contribution on the value.The serial dilutions of eight kinds of fenbutatin oxide 50WP is mixed with farnesol and nerolidol, dilution is administered to the female of organotin resistant strain and sensitive strain TSSM becomes on the mite.Each TSSM strain is carried out twice test, and test adopts 550 and the farnesol of 1000ppm and 450 and the nerolidol of 1000ppm, to be determined at the synergy degree of fenbutatin oxide 50WP under these variable concentrations.In addition, on responsive TSSM, use the farnesol of different proportion and nerolidol to carry out the biologicall test test to determine its influence to fenbutatin oxide 50WP.

In the previous day of handling, the female one-tenth mite of the TSSM of 12-15 sensitivity or organotin resistant strain is moved on to from the blade of being contaminated on the Kidney bean blade of diameter 15mm of cutting-out, above-mentioned blade is placed in " biologicall test cup " with on the moistening cotton balls of deionized water.

The serial spray dilution liquid of the composition of fenbutatin oxide 50WP and farnesol or nerolidol is to adopt the farnesol of same amount or the fenbutatin oxide 50WP of nerolidol solution and various dosage to prepare.With the fenbutatin oxide 50WP of following concentration and the composition of farnesol or nerolidol responsive tetranychid spraying is handled, the concentration of fenbutatin oxide is: 0.05,0.025,0.01,0.0005,0.0025,0.001,0.005 and 0.00025% active component.Biologicall test for the 550ppm farnesol, biologicall test to the 450ppm nerolidol, and to the biologicall test of 1000ppm, TSSM sprays with the active component of following percentage to the organotin resistance: 0.2324,0.1162,0.0465,0.0232,0.0116,0.0046,0.0023 and 0.0012.

Estimate at interval the lethality of responsive TSSM after the dispenser during (PSI) 4 days (96 hours).Because lethality is low in the time of PAI4 days, so organotin resistance TSSM is estimated when PAI4 and 6 days.Biometric data is expressed as with the form of tabulating and kills 50% (LC ₅₀) and 95% (LC ₉₅) for the ppm concentration (table 20 and 21) of planting experimentally the required active component of group.

Various processing and farnesol and nerolidol and LC are adopted in the ratio test on responsive TSSM ₅₀The composition of the fenbutatin oxide 50WP (194ppm) of value carries out.Used ratio is following, and (farnesol is preceding, nerolidol after, by ppm (ppm/ppm)): 150/150,100/200,75/225,30/270,200/100,225/75,270/30,120/180,180/120,300/0,0/300, add that the FCI-119 of a untreated control and 300pmm handles.Evaluation test when 4 days PAI.Biometric data is expressed as the average per cent death loss (table 22) of each processing with tabular form.

On responsive TSSM, farnesol and nerolidol be it seems the effectiveness that can both improve fenbutatin oxide 50WP.On the organotin resistant strain, the farnesol of 550ppm demonstrates the synergistic effect to fenbutatin oxide 50WP, and is more obvious when 1000ppm.The nerolidol of 450ppm be it seems to fenbutatin oxide 50WP inhibited, but has synergistic effect when 1000ppm.The ratio test of these two kinds of materials shows, the farnesol of higher proportion (i.e. the farnesol of big concentration) during with fenbutatin oxide 50WP combination to the activity of TSSM big (higher lethality).Adopting the processing 8 of 270/30ppm farnesol/nerolidol ratio is the processing (table 22) of lethality maximum.

After table 20. dispenser four days, the farnesol of two various dose and nerolidol are to the female LC that becomes mite of the responsive two-spotted spider mite of the miticide of handling with fenbutatin oxide 50WP ₅₀And LC ₉₅The influence of value

Compound	?LC 50(ppm)	??LC 95(ppm)
			Independent fenbutatin oxide	????194	????2195
Fenbutatin oxide+farnesol 1000ppm	????1.8	????19
			Fenbutatin oxide+nerolidol 1000ppm	????1.6	????24
Fenbutatin oxide+farnesol 550ppm	????10.7	????148
			Fenbutatin oxide+nerolidol 450ppm	????7.2	????350

The farnesol of two various dose of table 21. and nerolidol are to the female LC that becomes mite of the organotin resistance two-spotted spider mite of handling with fenbutatin oxide 50WP ₅₀And LC ₉₅The influence of value

Handle	4 days		6 days
					?LC 50	?LC 95	?LC 50	?LC 95
	Independent fenbutatin oxide	?2847	?I3366	?I730					?8201
Fenbutatin oxide+Fa Nechun @550ppm					?809	??-	?176	?4386
	Fenbutatin oxide+Fa Nechun @1000ppm	?723	??-	?91					?1549
Fenbutatin oxide+Cheng Huashuchun @450ppm					?3900	??-	?1768	??-
	Fenbutatin oxide+Cheng Huashuchun @1000ppm	?116	?914	?68					?485

The farnesol of the various dosage of table 22. and nerolidol are to the female effect that becomes the lethality of mite of the two-spotted spider mite of handling with fenbutatin oxide 50WP ¹⁰

Processing+farnesol/nerolidol, ppm	Average per cent death loss
		Untreated control	????8.75e
Fenbutatin oxide *+FCI-119	????33.50cd
		Fenbutatin oxide *+150/150	????25.00d
Fenbutatin oxide *+75/225	????30.75cd
		Fenbutatin oxide *+30/270	????37.25bcd
Fenbutatin oxide *+200/100	????45.00abc
		Fenbutatin oxide *+225/75	????48.75ab
Fenbutatin oxide *+270/30	????54.75a
		Fenbutatin oxide *+120/180	????50.00ab
Fenbutatin oxide *+180/120	????43.25abc
		Fenbutatin oxide *+300/0	????39.25bcd
Fenbutatin oxide *+0/300	????43.50abc

^*Dosage with 194ppm is used fenbutatin oxide

¹⁰According to DNMRT (P=0.05), the identical letter of mark means indifference statistically behind the mean value.

Embodiment 8: the two-spotted spider mite (Tetranychusurticae Koch) in organotin resistance and sensitive strain goes up the LC of the composition of FCI-119 and fenbutatin oxide 50WP to fenbutatin oxide ₅₀And LC ₉₅The influence of value

The purpose of present embodiment is on the two-spotted spider mite that is determined at organotin resistance and sensitive strain (Tetranychus urticae Koch), with the composition of fenbutatin oxide 50WP in use how much concentration FCI-119 can reduce the LC of the fenbutatin oxide 50WP of prior mensuration ₅₀And LC ₉₅Value.PSI provides the organotin resistant strain of TSSM, this strain be PSI in 1988 near Watsonville, separate in the commercial strawberry farm of CA.In embodiment 5, recorded FCI-119 and can improve the effectiveness of fenbutatin oxide 50WP significantly TSSM.The serial dilutions of eight kinds of fenbutatin oxide 50WP is mixed with FCI-119.Dilution is applied to the two-spotted spider mite of organotin resistant strain and sensitive strain is female to be become on the mite.Each TSSM strain is carried out three tests, use 1000,300 and the FCI-119 of 100ppm, to measure the synergy degree of these different concentration to fenbutatin oxide 50WP.

In the previous day of handling, on the female Kidney bean blade that becomes mite moves on to cutting-out from the blade that is infected 15mm diameter of the only responsive and TSSM of organotin resistant strain, above-mentioned blade is placed on to be used in " biologicall test cup " on the moistening cotton balls of deionized water with 12-15.

The serial dilution hydrojet of the composition of fenbutatin oxide 50WP and FCI-119 is to adopt the FCI-119 solution of same amount and the fenbutatin oxide 50WP of various dosage to prepare.With the composition of the fenbutatin oxide 50WP of following concentration and 1000ppm FCI-119 to the processing of spraying of responsive tetranychid: 0.05 0.025,0.01,0.005,0.0025,0.001,0.0005 and 0.00025% active component.With the composition of 0.2324,0.1162,0.0465,0.0232,0.0116,0.0046,0.0023 and 0.0012% active component and 1000ppm FCI-119 to the processing of spraying of organotin resistance tetranychid.Be administered on the responsive tetranychid with the FCI-119 of 300ppm with the fenbutatin oxide 50WP of 1000ppm test same concentrations.Handle the strain of organotin resistance with the composition spray of the fenbutatin oxide 50WP of following active component percentage and 300ppm FCI-119: 0.581,0.2905,0.1162,0.0581,0.02905,0.01162,0.00581 and 0.002905% active component.For responsive tetranychid, with the FCI-119 of 100ppm, mix with the fenbutatin oxide 50WP of following active component percentage: 0.1,0.05,0.025,0.01,0.005,0.0025,0.001 and 0.0005: the fenbutatin oxide that the organotin resistant strain is then mixed 1.162,0.581,0.2324,0.1162,0.0581,0.02324,0.01162 and 0.00581% active component.

For 1000 and the FCI-119 of 300ppm dosage, evaluation lethality during (PAI) 4 days (96 hours) and 6 days (144 hours) at interval after dispenser.Estimate the lethality that 100ppm FCI-119 handles when 4 days (96 hours) PAI, this only is because untreated control had higher lethality at the 6th day.Biometric data is expressed as with the form of tabulating and kills 50% (LC ₅₀) and 95% (LC ₉₅) for the ppm concentration (table 23) (move right four promptly percentage concentration is changed into ppm with decimal point) of planting experimentally the required active component of group.To each biologicall test, obtain the R value (i.e. at least 95% confidence level) of 0.95+ by Probability Analysis, show that the correlation of the tropic and original number is fine.

Data show that the FCI-119 that uses with 300ppm or 1000ppm dosage makes the obvious displacement of the tropic, and the result has improved the activity of fenbutatin oxide 50WP significantly.It seems that the FCI-119 of 100ppm can not make fenbutatin oxide 50WP synergy.

After table 23. dispenser four days, various dose FCI-119 is to the LC of the female one-tenth mite of two-spotted spider mite of usefulness fenbutatin oxide 50WP processing ₅₀And CL ₉₅The influence of value

Handle	Responsive (ppm)		Resistance (ppm)
					????LC 50	????LC 95	????LC 50	????LC 95
	Independent fenbutatin oxide	????252	????1157	????1163					????10596
Fenbutatin oxide+FCI-119 (100PPM)					????132	????988	????623	????8199
	Fenbutatin oxide+FCI-119 (300PPM)	????7	????34	????63					????929
Fenbutatin oxide+FCI-119 (1000PPM)					????20	????159	????68	????2580

Embodiment 9. farnesyl-methyl ethers become the effect on the activity of mite to responsive two-spotted spider mite (Tetranychusurticae Koch) at fenbutatin oxide

In the present embodiment, measured farnesyl-methyl ether (FME) when with the LC of prior mensuration ₅₀When the fenbutatin oxide 50WP combination of value is sprayed TSSM is become the effect of mite.Farnesyl-methyl ether (FME) is the analog of farnesol.

During in the biologicall test process each repeats, the previous day of handling, with the female one-tenth mite of 12-15 sensitive strain or sex mix if mite moves on to from the blade that is infected on the Kidney bean blade of diameter 15mm of cutting-out, above-mentioned blade is placed in " biologicall test cup " with on the moistening cotton balls of deionized water.

The serial dilutions of FME/ fenbutatin oxide 50WP is that the FME by the weighing aequum adds the anionic/nonionic surfactant (available from Witco ChemicalCorp., Houston, Armul 33 surfactants of Texas) that accounts for FME weight 5% and preparation.

The dosage that uses in fenbutatin oxide/FME test is as described below.The fenbutatin oxide 50WP of 200ppm is as the approximate LC that is obtained by biologicall test ₅₀Value.The fenbutatin oxide 50WP and 300 of 200ppm, 3 and the FME of 0.3ppm mix.Also spray 300,3 and the FME of 0.3ppm separately.Also comprise the processing of two independent 200ppm fenbutatin oxide 50WP.In addition, all tests all comprise untreated control.

Estimate the test of FME/ fenbutatin oxide when 4 days and 6 days PAI.Biometric data is expressed as the average per cent death loss (table 24) of each processing with the form of tabulation.

To the TSSM of sensitivity, the FME of FME, particularly 300 ppm has certain synergistic effect to fenbutatin oxide 50WP.It is identical (table 24) statistically that all FME handle with untreated control separately.

Table 24. with fenbutatin oxide 50WP composition in the farnesyl-methyl ether responsive two-spotted spider mite (T.urticae Koch) is become the influence of the lethality of mite ¹¹

Handle	Average per cent death loss
			4 days	6 days
	1. fenbutatin oxide 200ppm FME 300ppm	?81.6a			?93.0a
2. fenbutatin oxide 200ppm FME 3ppm			?39.2b	?61.3bc
	3. fenbutatin oxide 200ppm FME 0.3ppm	?49.3b			?72.6ab
?4.FME?300ppm			?15.1c	?29.5d
	?5.FME?3ppm	?10.3c			?33.1d
?6.FME?0.3ppm			?14.1c	?26.4d
	7. fenbutatin oxide 200ppm	?39.7b			?66.7b
8. fenbutatin oxide 200ppm			?37.8b	?38.9cd
	7. untreated control	?6.9c			?19.0d

¹¹According to DNMRT (P=0.05), the mean value back identical letter of mark means no significant difference.

It is believed that can improve when using miticide composition as herein described handles as crops such as clover, clover, cotton, peanut, jowar, citrus, soybean, blackberry, blueberry, rasp berry, corn, cucumber, muskmelon, pumpkin, custard squash, eggplant, pepper, tomato, hop, strawberry, ornamental plants, grape and fruit tree and nutwoods common miticide to as described in the level of preventing and treating of female and male tetranychid of miticide sensitivity or resistance.

The present invention is described with reference to its preferred embodiment, and these descriptions only are for illustration purpose, do not constitute limitation of the scope of the invention.Those skilled in the art can make various modifications and changes in the spirit and scope of the present invention that accessory claim limited.

Claims (19)

1. one kind is used to prevent and treat female, male and if the miticide composition of tetranychid population, described miticide composition comprises:

A kind of miticide is selected from: organotin, organophosphorus ester, imines (diamides), sulfite, macrolide, elementary sulfur and composition thereof; With

Improve the sesquiterpenoid of the effective dose of selected acaricidal toxicity action, be selected from: sesquiterpene alcohols, its analog and composition thereof.

2. according to the composition of claim 1, wherein miticide is an organotin acaricide.

3. according to the composition of claim 2, wherein organotin acaricide is a fenbutatin oxide.

4. according to the composition of claim 1, wherein miticide is the organophosphorus ester miticide.

5. according to the composition of claim 4, wherein the organophosphorus ester miticide is a zolone.

6. according to the composition of claim 1, wherein miticide is the imines miticide.

7. according to the composition of claim 6, wherein the imines miticide is an amitraz.

8. according to the composition of claim 1, wherein miticide is the sulfite miticide.

9. composition according to Claim 8, wherein the sulfite miticide is a propargite.

10. according to the composition of claim 1, wherein miticide is the macrolide miticide.

11. according to the composition of claim 10, wherein the macrolide miticide is an Olivomitecidin.

12. according to the composition of claim 1, wherein miticide is an elementary sulfur.

13. according to the composition of claim 1, wherein sesquiterpenoid is a sesquiterpene alcohols.

14. according to the composition of claim 13, wherein sesquiterpene alcohols is selected from farnesol, nerolidol and composition thereof.

15. composition according to claim 1, wherein the effective dose scope of sesquiterpenoid is that about 300ppm is to about 1000ppm in described composition, condition is when miticide is elementary sulfur, and the effective dose scope of sesquiterpenoid is that about 500ppm is to about 1000ppm in described composition.

16. if a control is female, male and the miticide composition of tetranychid population, described miticide composition comprises:

A kind of miticide is selected from: fenbutatin oxide, zolone, amitraz, propargite, Olivomitecidin, elementary sulfur and composition thereof; With

Improve the sesquiterpenoid of the effective dose of selected acaricidal toxicity action, be selected from: sesquiterpene alcohols, its analog and composition thereof.

17. according to the composition of claim 16, wherein sesquiterpenoid is a sesquiterpene alcohols.

18. according to the composition of claim 17, wherein sesquiterpene alcohols is selected from farnesol, nerolidol and composition thereof.

19. composition according to claim 16, wherein the effective dose scope of sesquiterpenoid is that about 300ppm is to about 1000ppm in described composition, condition is when miticide is elementary sulfur, and the effective dose scope of sesquiterpenoid is that about 500ppm is to about 1000ppm in described composition.