CN112500202A - Application of brown algae extract in preparation of agricultural fertilizer - Google Patents
Application of brown algae extract in preparation of agricultural fertilizer Download PDFInfo
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- CN112500202A CN112500202A CN201910872950.6A CN201910872950A CN112500202A CN 112500202 A CN112500202 A CN 112500202A CN 201910872950 A CN201910872950 A CN 201910872950A CN 112500202 A CN112500202 A CN 112500202A
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- brown algae
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- 241000199919 Phaeophyceae Species 0.000 title claims abstract description 50
- 239000000284 extract Substances 0.000 title claims abstract description 22
- 239000003337 fertilizer Substances 0.000 title claims description 14
- 238000002360 preparation method Methods 0.000 title claims description 6
- 238000003756 stirring Methods 0.000 claims abstract description 21
- 102000004190 Enzymes Human genes 0.000 claims abstract description 16
- 108090000790 Enzymes Proteins 0.000 claims abstract description 16
- 239000000843 powder Substances 0.000 claims abstract description 14
- 238000000034 method Methods 0.000 claims abstract description 10
- 229920001542 oligosaccharide Polymers 0.000 claims abstract description 9
- 102000004317 Lyases Human genes 0.000 claims abstract description 8
- 108090000856 Lyases Proteins 0.000 claims abstract description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 8
- CHHHXKFHOYLYRE-UHFFFAOYSA-M 2,4-Hexadienoic acid, potassium salt (1:1), (2E,4E)- Chemical compound [K+].CC=CC=CC([O-])=O CHHHXKFHOYLYRE-UHFFFAOYSA-M 0.000 claims abstract description 7
- 238000002156 mixing Methods 0.000 claims abstract description 7
- 229940069338 potassium sorbate Drugs 0.000 claims abstract description 7
- 235000010241 potassium sorbate Nutrition 0.000 claims abstract description 7
- 239000004302 potassium sorbate Substances 0.000 claims abstract description 7
- 239000002253 acid Substances 0.000 claims abstract description 5
- 239000002131 composite material Substances 0.000 claims abstract description 4
- 238000010438 heat treatment Methods 0.000 claims abstract description 4
- 238000005086 pumping Methods 0.000 claims abstract description 4
- 208000035240 Disease Resistance Diseases 0.000 claims description 7
- 229920000615 alginic acid Polymers 0.000 claims description 4
- 235000010443 alginic acid Nutrition 0.000 claims description 4
- 230000012010 growth Effects 0.000 claims description 4
- 239000011265 semifinished product Substances 0.000 claims description 4
- 230000004720 fertilization Effects 0.000 claims description 3
- 239000000203 mixture Substances 0.000 claims description 3
- 238000012545 processing Methods 0.000 claims description 3
- 238000012216 screening Methods 0.000 claims description 3
- 239000011550 stock solution Substances 0.000 claims description 3
- AEMOLEFTQBMNLQ-AZLKCVHYSA-N (2r,3s,4s,5s,6r)-3,4,5,6-tetrahydroxyoxane-2-carboxylic acid Chemical compound O[C@@H]1O[C@@H](C(O)=O)[C@@H](O)[C@H](O)[C@@H]1O AEMOLEFTQBMNLQ-AZLKCVHYSA-N 0.000 claims description 2
- AEMOLEFTQBMNLQ-SYJWYVCOSA-N (2s,3s,4s,5s,6r)-3,4,5,6-tetrahydroxyoxane-2-carboxylic acid Chemical compound O[C@@H]1O[C@H](C(O)=O)[C@@H](O)[C@H](O)[C@@H]1O AEMOLEFTQBMNLQ-SYJWYVCOSA-N 0.000 claims description 2
- 229940072056 alginate Drugs 0.000 claims description 2
- -1 alginate oligosaccharide Chemical class 0.000 claims description 2
- 150000002482 oligosaccharides Chemical class 0.000 abstract description 7
- 238000000605 extraction Methods 0.000 abstract 3
- 238000010298 pulverizing process Methods 0.000 abstract 1
- 239000002699 waste material Substances 0.000 abstract 1
- 239000000126 substance Substances 0.000 description 7
- SIKJAQJRHWYJAI-UHFFFAOYSA-N Indole Chemical compound C1=CC=C2NC=CC2=C1 SIKJAQJRHWYJAI-UHFFFAOYSA-N 0.000 description 4
- 229940079593 drug Drugs 0.000 description 3
- 239000003814 drug Substances 0.000 description 3
- 230000008635 plant growth Effects 0.000 description 3
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 2
- 229930192334 Auxin Natural products 0.000 description 2
- FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 description 2
- 229930195725 Mannitol Natural products 0.000 description 2
- 239000000783 alginic acid Substances 0.000 description 2
- 229960001126 alginic acid Drugs 0.000 description 2
- 150000004781 alginic acids Chemical class 0.000 description 2
- 239000002363 auxin Substances 0.000 description 2
- 239000011575 calcium Substances 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- PZOUSPYUWWUPPK-UHFFFAOYSA-N indole Natural products CC1=CC=CC2=C1C=CN2 PZOUSPYUWWUPPK-UHFFFAOYSA-N 0.000 description 2
- SEOVTRFCIGRIMH-UHFFFAOYSA-N indole-3-acetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CNC2=C1 SEOVTRFCIGRIMH-UHFFFAOYSA-N 0.000 description 2
- RKJUIXBNRJVNHR-UHFFFAOYSA-N indolenine Natural products C1=CC=C2CC=NC2=C1 RKJUIXBNRJVNHR-UHFFFAOYSA-N 0.000 description 2
- 239000000594 mannitol Substances 0.000 description 2
- 235000010355 mannitol Nutrition 0.000 description 2
- 239000005416 organic matter Substances 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 230000001737 promoting effect Effects 0.000 description 2
- ZOXJGFHDIHLPTG-UHFFFAOYSA-N Boron Chemical compound [B] ZOXJGFHDIHLPTG-UHFFFAOYSA-N 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 108090000695 Cytokines Proteins 0.000 description 1
- 102000004127 Cytokines Human genes 0.000 description 1
- AUNGANRZJHBGPY-UHFFFAOYSA-N D-Lyxoflavin Natural products OCC(O)C(O)C(O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-UHFFFAOYSA-N 0.000 description 1
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 description 1
- AUNGANRZJHBGPY-SCRDCRAPSA-N Riboflavin Chemical compound OC[C@@H](O)[C@@H](O)[C@@H](O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-SCRDCRAPSA-N 0.000 description 1
- 229930003451 Vitamin B1 Natural products 0.000 description 1
- 229930003779 Vitamin B12 Natural products 0.000 description 1
- 229930003471 Vitamin B2 Natural products 0.000 description 1
- 229930003268 Vitamin C Natural products 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 230000000975 bioactive effect Effects 0.000 description 1
- 229910052796 boron Inorganic materials 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- FDJOLVPMNUYSCM-WZHZPDAFSA-L cobalt(3+);[(2r,3s,4r,5s)-5-(5,6-dimethylbenzimidazol-1-yl)-4-hydroxy-2-(hydroxymethyl)oxolan-3-yl] [(2r)-1-[3-[(1r,2r,3r,4z,7s,9z,12s,13s,14z,17s,18s,19r)-2,13,18-tris(2-amino-2-oxoethyl)-7,12,17-tris(3-amino-3-oxopropyl)-3,5,8,8,13,15,18,19-octamethyl-2 Chemical compound [Co+3].N#[C-].N([C@@H]([C@]1(C)[N-]\C([C@H]([C@@]1(CC(N)=O)C)CCC(N)=O)=C(\C)/C1=N/C([C@H]([C@@]1(CC(N)=O)C)CCC(N)=O)=C\C1=N\C([C@H](C1(C)C)CCC(N)=O)=C/1C)[C@@H]2CC(N)=O)=C\1[C@]2(C)CCC(=O)NC[C@@H](C)OP([O-])(=O)O[C@H]1[C@@H](O)[C@@H](N2C3=CC(C)=C(C)C=C3N=C2)O[C@@H]1CO FDJOLVPMNUYSCM-WZHZPDAFSA-L 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 239000004062 cytokinin Substances 0.000 description 1
- UQHKFADEQIVWID-UHFFFAOYSA-N cytokinin Natural products C1=NC=2C(NCC=C(CO)C)=NC=NC=2N1C1CC(O)C(CO)O1 UQHKFADEQIVWID-UHFFFAOYSA-N 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000005059 dormancy Effects 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000004345 fruit ripening Effects 0.000 description 1
- 230000035784 germination Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 150000004804 polysaccharides Chemical class 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 229960002477 riboflavin Drugs 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 229960003495 thiamine Drugs 0.000 description 1
- DPJRMOMPQZCRJU-UHFFFAOYSA-M thiamine hydrochloride Chemical compound Cl.[Cl-].CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N DPJRMOMPQZCRJU-UHFFFAOYSA-M 0.000 description 1
- 238000012795 verification Methods 0.000 description 1
- 235000010374 vitamin B1 Nutrition 0.000 description 1
- 239000011691 vitamin B1 Substances 0.000 description 1
- 235000019163 vitamin B12 Nutrition 0.000 description 1
- 239000011715 vitamin B12 Substances 0.000 description 1
- 235000019164 vitamin B2 Nutrition 0.000 description 1
- 239000011716 vitamin B2 Substances 0.000 description 1
- 235000019154 vitamin C Nutrition 0.000 description 1
- 239000011718 vitamin C Substances 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Fertilizers (AREA)
Abstract
The invention relates to the field of brown algae extracts, in particular to a brown algae oligosaccharide extraction method. Aims to solve the problems of complicated process, high cost, low purity of extraction and great waste of the existing brown algae extraction technology. Comprises deep sea brown algae 12.65%, pure water 80%, mixed enzyme 7%, lyase 0.1%, CZK30 complex enzyme 0.15%, and potassium sorbate 0.1%; the method comprises the following seven steps: 1. pulverizing deep-sea brown algae 12.65%; 2. pumping the deep-sea brown algae powder obtained in the step 1 into a stirring tank; 3. adding 80% pure water and deep sea brown algae powder into a stirring tank, mixing and stirring; 4. heating the half finished product obtained in the step 1 to the step three to 40 ℃ and uniformly stirring at a uniform speed; 5. performing multi-step composite moderate-temperature weak acid biological enzymolysis, firstly adding 0.1% of lyase at the moderate temperature of 40 ℃ for starting enzymolysis, wherein the enzymolysis time is 2 hours, then adding 0.15% of CZK30 complex enzyme for enzymolysis for 5 hours, and finally adding 7% of mixed enzyme for enzymolysis for 5 hours; 6. detecting and adjusting the pH value; 7. and (5) separating.
Description
Technical Field
The invention relates to the field of fertilizers, in particular to application of brown algae extract in preparation of agricultural fertilizers.
Background
The brown algae extract brown algae oligosaccharide is used in the agricultural industry and has the following advantages: the environment compatibility is good (the material is easy to decompose in the environment, has no residual influence, and has no adverse influence on the environment and ecological balance). Has good safety (the raw material is from natural marine plants, and has no chemical pollution). The disease resistance of the plants can be improved, the plants can be induced to generate disease resistance, the disease resistance of the plants is improved, and the application amount of chemical medicines can be reduced; meanwhile, the growth of plants is promoted, and the dormancy of seeds can be broken to promote germination; promoting the growth of roots and stems and leaves; preserving flowers and fruits; promoting fruit ripening.
Disclosure of Invention
The invention aims to apply the brown algae extract to the fertilizer in the agricultural industry, change the pollution of the existing chemical drugs to the environment, improve the disease resistance of the plant growth and promote the growth.
In order to solve the technical problems, the brown algae extract is used for preparing agricultural fertilizers, is a linear oligomer composed of beta-D-mannuronic acid and alpha-L-guluronic acid, and can improve the disease resistance of plants and promote the growth of the plants;
mixing the brown algae extract with a fertilizer for plant fertilization;
the preparation method of the brown algae extract comprises the following steps: mixing dried brown algae 12.65%, pure water 80%, mixed enzyme 7%, lyase 0.1%, CZK30 complex enzyme 0.15%, and potassium sorbate 0.1%;
the method comprises the following seven steps:
step one, breaking the wall of 12.65% dry brown algae to 200 meshes by high-power dust-free crushing, and the step comprises the following three procedures:
1) feeding dry brown algae to high-power dust-free crushing,
2) the processing from coarse crushing to fine crushing is carried out,
3) screening and detecting the powder after wall breaking, and entering the next step if the powder is qualified;
step two, pumping the dry brown algae powder obtained in the step 1 into a stirring tank,
step three, adding 80% of pure water and dry brown algae powder into a stirring tank, and mixing and stirring;
step four, heating the semi-finished products obtained in the steps 1 to three to 40 ℃, uniformly stirring at a uniform speed,
step five, carrying out multi-step composite moderate-temperature weak acid biological enzymolysis, wherein the step comprises the following three procedures:
1) adding 0.1% of lyase at the medium temperature of 40 ℃ to start enzymolysis, wherein the enzymolysis time is 2 hours,
2) then 0.15 percent of CZK30 complex enzyme is added for enzymolysis for 5 hours,
3) finally adding 7% of mixed enzyme for enzymolysis for 5 hours;
step six, detecting and adjusting the pH value, wherein the pH value is 7;
and seventhly, adding 0.1 percent of potassium sorbate, stirring uniformly, and pushing the mixture to a centrifuge to separate out the brown algae extract stock solution from the dry brown algae.
Has the advantages that:
the invention has the following innovative advantages,
The brown algae extract is applied to the fertilizer, so that the pollution of the existing chemical drugs to the environment can be reduced, the plant growth can be better promoted, the disease resistance can be improved, and the plant planting cost can be reduced.
Drawings
FIG. 1 is a flow chart of the present invention;
FIG. 2 is a diagram showing the components of COA detection according to the present invention.
Detailed Description
The invention is further illustrated by the following figures and examples.
The brown algae extract provided by the invention is used for preparing agricultural fertilizers, and through multi-step compound medium-temperature weak-acid biological enzymolysis, the extracted brown algae extract brown algae oligosaccharide is purer, higher in peptide and sugar activities and more thorough in enzymolysis, so that bioactive substances and nutrient substances in brown algae are retained to the maximum extent, polysaccharide substances are degraded into extremely fine oligosaccharides, the compatibility is good, and plants are easy to absorb.
As shown in fig. 1, the brown algae extract of the present invention is used for preparing agricultural fertilizer, and the brown algae extract brown algae oligosaccharide is mixed with fertilizer for plant fertilization, wherein the preparation method of the brown algae extract comprises: comprises 12.65% of dry brown algae, 80% of pure water, 7% of mixed enzyme, 0.1% of lyase, 0.15% of CZK30 complex enzyme and 0.1% of potassium sorbate;
the manufacturing process comprises the following steps: breaking the wall of 12.65% dry brown algae to 200 meshes by high-power dust-free crushing, and the method comprises the following three steps:
1) feeding dry brown algae to high-power dust-free crushing,
2) the processing from coarse crushing to fine crushing is carried out,
3) screening and detecting the powder after wall breaking, and entering the next step if the powder is qualified;
pumping the crushed dry brown algae powder into a stirring tank for stirring,
then 80% pure water and dry brown algae powder are added into the stirring tank to be mixed and stirred,
heating the semi-finished product obtained in the above steps to 40 ℃ after stirring, uniformly stirring at a uniform speed,
stirring and then carrying out multi-step composite moderate-temperature weak acid biological enzymolysis, wherein the steps comprise the following three procedures:
1) under the condition of keeping at the medium temperature of 40 ℃, 0.1 percent of lyase is added to start enzymolysis, the enzymolysis time is 2 hours,
2) then 0.15 percent of CZK30 complex enzyme is added for enzymolysis for 5 hours,
3) finally adding 7% of mixed enzyme for enzymolysis for 5 hours;
detecting after completion, performing enzymolysis again if the product is unqualified, entering the next step if the product is qualified, adjusting the pH value to 7,
and finally, separating, wherein the separation process comprises the following steps: and adding 0.1% of potassium sorbate into the semi-finished product obtained in the step again, uniformly stirring, and pushing the mixture to a centrifuge to separate out brown alginate oligosaccharide stock solution from dry brown algae.
As shown in FIG. 2, the brown algae extract of the present invention is used for preparing agricultural fertilizers,
wherein the brown algae extract has the following components by the verification of Analysis (COA) detection Analysis:
indole Auxin/Indole Auxin 3ppm
Endogenous Cytokinins/cytokines 0.012ppm
Vitamin B1/vb 123 ppm
Vitamin B2/v B215 ppm
Vitamin B12/Vb120.02ppm
Vitamin C/Vc 900ppm
Organic matter/Organic matter 55 g/L
Oligosaccharide/Oligosaccharide 38 g/L
Alginic acid/Alginic acid 3g/L
Mannitol/Mannitol 23 g/L
boron/B3.2 g/L
calcium/Ca 4.1 g/L
The above-described preferred embodiments of the present invention are not intended to limit the present invention, and any modifications made within the spirit and principle of the present invention should be included within the scope of the present invention.
Claims (1)
1. The brown algae extract is used for preparing agricultural fertilizer, and is characterized in that the brown algae extract is a linear oligomer consisting of beta-D-mannuronic acid and alpha-L-guluronic acid, and can improve the disease resistance of plants and promote the growth of the plants;
mixing the brown algae extract with a fertilizer for plant fertilization;
the preparation method of the brown algae extract comprises the following steps: mixing dried brown algae 12.65%, pure water 80%, mixed enzyme 7%, lyase 0.1%, CZK30 complex enzyme 0.15%, and potassium sorbate 0.1%;
the method comprises the following seven steps:
step one, breaking the wall of 12.65% dry brown algae to 200 meshes by high-power dust-free crushing, and the step comprises the following three procedures:
1) feeding dry brown algae to high-power dust-free crushing,
2) the processing from coarse crushing to fine crushing is carried out,
3) screening and detecting the powder after wall breaking, and entering the next step if the powder is qualified;
step two, pumping the dry brown algae powder obtained in the step 1 into a stirring tank,
step three, adding 80% of pure water and dry brown algae powder into a stirring tank, and mixing and stirring;
step four, heating the semi-finished products obtained in the steps 1 to three to 40 ℃, uniformly stirring at a uniform speed,
step five, carrying out multi-step composite moderate-temperature weak acid biological enzymolysis, wherein the step comprises the following three procedures:
1) adding 0.1% of lyase at the medium temperature of 40 ℃ to start enzymolysis, wherein the enzymolysis time is 2 hours,
2) then 0.15 percent of CZK30 complex enzyme is added for enzymolysis for 5 hours,
3) finally adding 7% of mixed enzyme for enzymolysis for 5 hours;
step six, detecting and adjusting the pH value, wherein the pH value is 7;
and seventhly, adding 0.1% of potassium sorbate, stirring uniformly, and pushing the mixture to a centrifuge to separate out the alginate oligosaccharide stock solution from the dry brown algae.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910872950.6A CN112500202A (en) | 2019-09-16 | 2019-09-16 | Application of brown algae extract in preparation of agricultural fertilizer |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910872950.6A CN112500202A (en) | 2019-09-16 | 2019-09-16 | Application of brown algae extract in preparation of agricultural fertilizer |
Publications (1)
| Publication Number | Publication Date |
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| CN112500202A true CN112500202A (en) | 2021-03-16 |
Family
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| CN201910872950.6A Pending CN112500202A (en) | 2019-09-16 | 2019-09-16 | Application of brown algae extract in preparation of agricultural fertilizer |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113929532A (en) * | 2021-11-25 | 2022-01-14 | 深圳市芭田生态工程股份有限公司 | Brown algae oligosaccharide water-soluble fertilizer and preparation method thereof |
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Application publication date: 20210316 |