CN112485249A - Kit for visual rapid detection of ethanol content in disinfectant and detection method - Google Patents
Kit for visual rapid detection of ethanol content in disinfectant and detection method Download PDFInfo
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- CN112485249A CN112485249A CN202011364630.9A CN202011364630A CN112485249A CN 112485249 A CN112485249 A CN 112485249A CN 202011364630 A CN202011364630 A CN 202011364630A CN 112485249 A CN112485249 A CN 112485249A
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- disinfectant
- kit
- ethanol content
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- ethanol
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N2021/775—Indicator and selective membrane
Abstract
The invention relates to a kit for visually and rapidly detecting the content of ethanol in a disinfectant and a detection method, wherein the kit comprises a lower box (1) and an upper box (2), a sponge pad (3) is arranged in the lower box (1), a plurality of jacks (4) and a sampling tank (5) are formed in the sponge pad (3), a centrifugal tube (6) is inserted into the jacks (4), a quantitative sampling needle (7) is arranged in the sampling tank (5), a layer of waterproof aluminum foil glass fiber cloth (8) is arranged on the inner wall of the upper box (2), a middle partition plate (9) is arranged in the upper box (2), and a plurality of positioning holes (10) are formed in the middle partition plate (9). The kit and the detection method for visually and rapidly detecting the ethanol content in the disinfectant have the advantages of simple operation and low cost, and can directly visually identify whether the content of the 75% ethanol reaches the standard or not without any instrument and equipment.
Description
Technical Field
The invention relates to a kit for visually and rapidly detecting the content of ethanol in a disinfectant and a detection method, and belongs to the technical field of chemical rapid detection.
Background
2019 novel coronavirus (2019-nCoV), due to its extremely strong infectivity, causes epidemic outbreaks and rapid epidemics in China and other countries. 2019-nCoV is a single-stranded positive-strand RNA virus of the genus Beta, order Neuroviridae, family Coronaviridae. The virus is sensitive to ultraviolet rays and heat, and can be effectively killed by diethyl ether, 75% ethanol, chlorine-containing disinfectant, peracetic acid, chloroform and the like at the temperature of 56 ℃ for 30 min. Ultraviolet ray, chlorine-containing disinfectant and peracetic acid are suitable for disinfection of places and are not suitable for people. Chloroform is highly toxic and has little practical application. The 75% ethanol has low toxicity and is volatile, is suitable for local disinfection of common people and medical workers, and is widely used. However, if the alcohol content in the disinfectant is too low, 2019-nCoV is killed, so that the quality of the 75% alcohol disinfectant sold in the market is necessary to be effectively regulated. According to the specification of the disinfectant (2002 edition), methods for detecting the ethanol content in the disinfectant comprise gas chromatography and a hydrometer method. The gas chromatography detection is accurate, but needs large instruments and professional personnel to operate, and the whole process has long detection period and high cost. Hydrometric methods can only detect solutions containing only ethanol and water, and cannot be used if the disinfectant contains other components. In order to strengthen the supervision of the related departments on the quality of the 75 percent ethanol disinfectant, the method which is simple to operate, low in cost and rapid to visualize on site is established, and has practical significance.
Disclosure of Invention
The invention aims to solve the technical problem of providing a kit and a detection method for quickly detecting the ethanol content in a disinfectant by visual inspection, which are simple in operation and low in cost, aiming at the prior art, and can directly identify whether the ethanol content reaches the standard by visual inspection without any instrument and equipment.
The technical scheme adopted by the invention for solving the problems is as follows: the utility model provides a visual quick detection disinfectant mesoethanol's kit, it includes box and last box down, be provided with a foam-rubber cushion in the box down, a plurality of jacks and a sample tank have been seted up on the foam-rubber cushion, the centrifuging tube has been equipped with in the jack interpolation, be provided with quantitative sampling needle in the sample tank, be provided with the fine cloth of waterproof aluminium foil glass of one deck on the inner wall of going up the box, be provided with intermediate bottom in going up the box, a plurality of locating holes have been seted up on the intermediate bottom, the position one-to-one of a plurality of jacks and a plurality of locating holes, centrifuging tube upper end cartridge is in the locating.
Optionally, the size of the lower box is 20cm in length, 10cm in width and 5cm in height, and the size of the spongy cushion is consistent with that of the lower box.
Optionally, the size of the upper box is 20.3cm long, 10.3cm wide and 5.5cm high.
Optionally, the diameter of the insertion hole is 1.2cm, and the depth of the insertion hole is 4.3 cm.
Optionally, the length of the sampler groove is 13cm, the width of the sampler groove is 0.4cm, and the depth of the sampler groove is 2 cm.
Optionally, the length of the middle partition board is 20cm, the width of the middle partition board is 10cm, and the thickness of the middle partition board is 0.4 cm.
Optionally, the middle partition plate is arranged at a position 1cm away from the opening of the upper box 2.
Optionally, the intermediate partition is made of a polypropylene material.
Optionally, the diameter of the positioning hole is 1.1 cm.
A method for detecting a kit for visually and rapidly detecting the ethanol content in a disinfectant, which comprises the following steps:
step one, weighing 1.25g of potassium dichromate, metering the volume to 30ml by using 40% phosphoric acid, and accurately sucking 1.5ml of solution to a 1.5ml centrifuge tube, wherein the total amount is 20 parts; taking 2ml of 75% ethanol standard solution, and putting 1 part of the solution in a 2ml centrifuge tube;
taking out two centrifuge tubes filled with potassium dichromate solution, respectively sucking 25 mul of 75% ethanol standard solution and 25 mul of disinfectant sample into the two centrifuge tubes by using 25 mul of quantitative sampling needles, and covering the centrifuge tubes with covers to shake the centrifuge tubes evenly;
step three, inserting the two centrifuge tubes in the step two into positioning holes of the upper box, pouring boiling water into the upper box to the position of the middle partition plate, reacting for 5min, and taking out the two centrifuge tubes for color comparison;
if the sample is equivalent to the 75% ethanol standard solution and is clear and emerald green, the ethanol content of the disinfectant reaches 75%, and the disinfectant is qualified;
if the sample is green and orange, the ethanol content of the disinfectant is less than 75 percent, and the sample is unqualified.
Compared with the prior art, the invention has the advantages that:
1. the kit for detecting the ethanol content in the 2019-nCoV disinfectant, which is prepared by the invention, does not need any instrument, can be observed in a visual color, and is suitable for field detection;
2. the finished kit prepared by the invention is simple to operate in actual use, does not need professional staff and is low in cost;
3. the finished kit prepared by the invention only needs one-step reaction, and results are obtained after 5min, so that the time consumption is short, and the efficiency is high.
Drawings
FIG. 1 is a schematic structural diagram of a kit for rapidly detecting ethanol content in a disinfectant by visual inspection according to the present invention.
Fig. 2 is a top view of the lower cassette of fig. 1.
Wherein:
Jack 4
Sampler tank 5
Centrifuge tube 6
Waterproof aluminum foil glass fiber cloth 8
The positioning holes 10.
Detailed Description
The invention is described in further detail below with reference to the accompanying examples.
As shown in fig. 1 and 2, the kit for visually and rapidly detecting the ethanol content in a disinfectant comprises a lower box 1 and an upper box 2, wherein a sponge pad 3 is arranged in the lower box 1, a plurality of jacks 4 and a sampling tank 5 are formed in the sponge pad 3, a centrifuge tube 6 is inserted into the jacks 4, a quantitative sampling needle 7 is arranged in the sampling tank 5, a layer of waterproof aluminum foil glass fiber cloth 8 is arranged on the inner wall of the upper box 2, a middle partition plate 9 is arranged in the upper box 2, a plurality of positioning holes 10 are formed in the middle partition plate 9, the jacks 4 correspond to the positioning holes 10 one by one, and the upper end of the centrifuge tube 6 is inserted into the positioning holes 10;
the lower box 1 is 20cm long, 10cm wide and 5cm high;
the upper box 2 is 20.3cm long, 10.3cm wide and 5.5cm high;
the size of the spongy cushion 3 is consistent with that of the lower box 1;
the diameter of the jack 4 is 1.2cm, and the depth is 4.3 cm;
the length of the sampler groove 5 is 13cm, the width of the sampler groove is 0.4cm, and the depth of the sampler groove is 2 cm;
the number of the jacks 4 is 21, wherein 1.5ml of centrifuge tubes 6 are inserted into 20 jacks 4, and 2ml of centrifuge tubes 6 are inserted into the rest 1 jack 4;
the quantitative sampling needle 7 adopts a 25 mu l quantitative sampling needle;
the middle partition board 9 is 20cm in length, 10cm in width and 0.4cm in thickness;
the middle partition plate 9 is arranged at a position 1cm away from the box opening of the upper box 2;
the middle partition plate 9 is made of polypropylene material;
the diameter of the positioning hole 10 is 1.1 cm.
The detection method comprises the following steps:
step one, weighing 1.25g of potassium dichromate, metering the volume to 30ml by using 40% phosphoric acid, and accurately sucking 1.5ml of solution to a 1.5ml centrifuge tube, wherein the total amount is 20 parts; taking 2ml of 75% ethanol standard solution, and putting 1 part of the solution in a 2ml centrifuge tube;
taking out two centrifuge tubes filled with potassium dichromate solution, respectively sucking 25 mul of 75% ethanol standard solution and 25 mul of disinfectant sample into the two centrifuge tubes by using 25 mul of quantitative sampling needles, and covering the centrifuge tubes with covers to shake the centrifuge tubes evenly;
step three, inserting the two centrifuge tubes in the step two into positioning holes of the upper box, pouring boiling water into the upper box to the position of the middle partition plate, reacting for 5min, and taking out the two centrifuge tubes for color comparison;
if the sample is equivalent to the 75% ethanol standard solution and is clear and emerald green, the ethanol content of the disinfectant reaches 75%, and the disinfectant is qualified;
if the sample is green and orange, the ethanol content of the disinfectant is less than 75 percent, and the sample is unqualified. Wherein the ethanol content is 0% orange, the color gradually changes from orange to green after the ethanol content is 25%, 50% and 70% react, and the ethanol content is 75% and is just clear emerald green.
In addition to the above embodiments, the present invention also includes other embodiments, and any technical solutions formed by equivalent transformation or equivalent replacement should fall within the scope of the claims of the present invention.
Claims (10)
1. A kit for visual rapid detection of ethanol content in disinfectant is characterized in that: it includes box (1) and last box (2) down, be provided with a foam-rubber cushion (3) in box (1) down, a plurality of jacks (4) and a sample tank (5) have been seted up on foam-rubber cushion (3), centrifuging tube (6) have been equipped with in jack (4) interpolation, be provided with quantitative sampling needle (7) in sample tank (5), be provided with the fine cloth (8) of waterproof aluminium foil glass of one deck on the inner wall of going up box (2), be provided with intermediate bottom plate (9) in going up box (2), a plurality of locating holes (10) have been seted up on intermediate bottom plate (9), the position one-to-one of a plurality of jacks (4) and a plurality of locating holes (10), centrifuging tube (6) upper end cartridge is in locating hole (10).
2. The kit for visual rapid detection of ethanol content in disinfectant according to claim 1, wherein: the size of the lower box (1) is 20cm in length, 10cm in width and 5cm in height, and the size of the spongy cushion (3) is consistent with that of the lower box (1).
3. The kit for visual rapid detection of ethanol content in disinfectant according to claim 1, wherein: the upper box (2) has the dimensions of 20.3cm in length, 10.3cm in width and 5.5cm in height.
4. The kit for visual rapid detection of ethanol content in disinfectant according to claim 1, wherein: the diameter of the jack (4) is 1.2cm, and the depth is 4.3 cm.
5. The kit for visual rapid detection of ethanol content in disinfectant according to claim 1, wherein: the length of the sampler groove (5) is 13cm, the width is 0.4cm, and the depth is 2 cm.
6. The kit for visual rapid detection of ethanol content in disinfectant according to claim 1, wherein: the length of the middle partition board (9) is 20cm, the width of the middle partition board is 10cm, and the thickness of the middle partition board is 0.4 cm.
7. The kit for visual rapid detection of ethanol content in disinfectant according to claim 1, wherein: the middle partition plate (9) is arranged at a position 1cm away from the box opening of the upper box (2).
8. The kit for visual rapid detection of ethanol content in disinfectant according to claim 1, wherein: the middle partition plate (9) is made of polypropylene materials.
9. The kit for visual rapid detection of ethanol content in disinfectant according to claim 1, wherein: the diameter of the positioning hole (10) is 1.1 cm.
10. The detection method of the kit for visually and rapidly detecting the ethanol content in the disinfectant is characterized by comprising the following steps of:
step one, weighing 1.25g of potassium dichromate, metering the volume to 30ml by using 40% phosphoric acid, and accurately sucking 1.5ml of solution to a 1.5ml centrifuge tube, wherein the total amount is 20 parts; taking 2ml of 75% ethanol standard solution, and putting 1 part of the solution in a 2ml centrifuge tube;
taking out two centrifuge tubes filled with potassium dichromate solution, respectively sucking 25 mul of 75% ethanol standard solution and 25 mul of disinfectant sample into the two centrifuge tubes by using 25 mul of quantitative sampling needles, and covering the centrifuge tubes with covers to shake the centrifuge tubes evenly;
step three, inserting the two centrifuge tubes in the step two into positioning holes of the upper box, pouring boiling water into the upper box to the position of the middle partition plate, reacting for 5min, and taking out the two centrifuge tubes for color comparison;
if the sample is equivalent to the 75% ethanol standard solution and is clear and emerald green, the ethanol content of the disinfectant reaches 75%, and the disinfectant is qualified;
if the sample is green and orange, the ethanol content of the disinfectant is less than 75 percent, and the sample is unqualified.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011364630.9A CN112485249B (en) | 2020-11-27 | 2020-11-27 | Kit for quickly detecting ethanol content in disinfectant through visual inspection and detection method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011364630.9A CN112485249B (en) | 2020-11-27 | 2020-11-27 | Kit for quickly detecting ethanol content in disinfectant through visual inspection and detection method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN112485249A true CN112485249A (en) | 2021-03-12 |
| CN112485249B CN112485249B (en) | 2022-11-25 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202011364630.9A Active CN112485249B (en) | 2020-11-27 | 2020-11-27 | Kit for quickly detecting ethanol content in disinfectant through visual inspection and detection method |
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Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201518024U (en) * | 2009-05-08 | 2010-06-30 | 福州蓝昊生物医药有限公司 | Quick melamine detection kit |
| CN101825576A (en) * | 2009-11-30 | 2010-09-08 | 无锡灵特生物技术有限责任公司 | Method and kit for rapid detection of ethanol content in microbial fermentation solution |
| CN103592238A (en) * | 2013-10-08 | 2014-02-19 | 绍兴圣康生物科技有限公司 | Serum ethanol content determination kit |
| CN204607989U (en) * | 2015-05-12 | 2015-09-02 | 上海允英医疗科技有限公司 | A kind of test kit of full RAS gene test |
| CN205077054U (en) * | 2015-09-29 | 2016-03-09 | 郭旭光 | Detect kit of no streptococcus lactis |
| CN207876315U (en) * | 2018-02-05 | 2018-09-18 | 天津威高分子诊断科技有限公司 | A kind of modified form HCV virus parting detecting reagent |
| CN208520761U (en) * | 2017-06-09 | 2019-02-19 | 北京中生朗捷生物技术股份有限公司 | For saliva, the sample detecting stick and kit of DNA acquisition and alcohol detection |
-
2020
- 2020-11-27 CN CN202011364630.9A patent/CN112485249B/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201518024U (en) * | 2009-05-08 | 2010-06-30 | 福州蓝昊生物医药有限公司 | Quick melamine detection kit |
| CN101825576A (en) * | 2009-11-30 | 2010-09-08 | 无锡灵特生物技术有限责任公司 | Method and kit for rapid detection of ethanol content in microbial fermentation solution |
| CN103592238A (en) * | 2013-10-08 | 2014-02-19 | 绍兴圣康生物科技有限公司 | Serum ethanol content determination kit |
| CN204607989U (en) * | 2015-05-12 | 2015-09-02 | 上海允英医疗科技有限公司 | A kind of test kit of full RAS gene test |
| CN205077054U (en) * | 2015-09-29 | 2016-03-09 | 郭旭光 | Detect kit of no streptococcus lactis |
| CN208520761U (en) * | 2017-06-09 | 2019-02-19 | 北京中生朗捷生物技术股份有限公司 | For saliva, the sample detecting stick and kit of DNA acquisition and alcohol detection |
| CN207876315U (en) * | 2018-02-05 | 2018-09-18 | 天津威高分子诊断科技有限公司 | A kind of modified form HCV virus parting detecting reagent |
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|---|---|
| CN112485249B (en) | 2022-11-25 |
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