CN111044601A - Soil or sediment denitrification in-situ determination device and determination method in flooded environment - Google Patents

Soil or sediment denitrification in-situ determination device and determination method in flooded environment Download PDF

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CN111044601A
CN111044601A CN201911416804.9A CN201911416804A CN111044601A CN 111044601 A CN111044601 A CN 111044601A CN 201911416804 A CN201911416804 A CN 201911416804A CN 111044601 A CN111044601 A CN 111044601A
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pvc
drum
soil
stainless steel
denitrification
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周伟
颜晓元
赵旭
李晓明
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Institute of Soil Science of CAS
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Institute of Soil Science of CAS
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/62—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating the ionisation of gases, e.g. aerosols; by investigating electric discharges, e.g. emission of cathode
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/02—Devices for withdrawing samples
    • G01N1/10—Devices for withdrawing samples in the liquid or fluent state
    • G01N1/14—Suction devices, e.g. pumps; Ejector devices

Abstract

A denitrification in-situ measuring device and a denitrification in-situ measuring method for soil or sediment in a flooded environment belong to the technical field of denitrification in-situ quantification. The device includes stainless steel handle, injection piston, PVC drum, stainless steel drum and the transparent bottle of PE, and the top of stainless steel drum is located to PVC drum and stainless steel drum intercommunication and PVC drum, and PVC drum bottom outside is located to the stainless steel handle, and injection piston swing joint is inside the PVC drum, and the transparent bottle of PE is top opening, the airtight and inside hollow ring form in bottom, and the PVC drum outside is located to the transparent bottle cover of PE. The device can establish a sealed environment in a field environment, and avoids N in the atmosphere2The interference of (3) ensures that environmental conditions are consistent with the natural environment, and the sampling is lossless through pressing the piston, so that a tank body is not required to be damaged, a sealed environment can be maintained, water sampling can be accurately metered, the operation is simple and accurate, and finally the denitrification of soil or sediments in a flooding environment is realizedIn situ determination of the rate.

Description

Soil or sediment denitrification in-situ determination device and determination method in flooded environment
Technical Field
The invention relates to the technical field of denitrification, in particular to a denitrification in-situ measuring device and a denitrification in-situ measuring method for soil or sediments in a flooded environment.
Background
Denitrification enables the production of Nitrate (NO)3 --N) conversion to N2Returning to the atmospheric nitrogen reservoir is one of the main ways for removing active nitrogen in the natural flooding environment. Under flooding conditions, the main product of denitrification is nitrogen (N)2) Over 90% of the total product, and 78% of the atmospheric constituents are N2The concentration of natural background is very high, therefore, strict sealing conditions are required to avoid the generation of bubbles when measuring denitrification so as to ensure that the sample is not influenced by the external N2The interference brings great problems to the quantitative determination of the denitrification of the soil or the sediment in the flooding environment. At present, most of the existing methods are to collect undisturbed soil columns and bring the undisturbed soil columns back to the indoor for simulation culture, a large amount of soil columns need to be collected in the field and transported back to a laboratory, and the workload is huge. In addition, during indoor simulation culture, the environmental conditions such as temperature, dissolved oxygen and the like closely related to denitrification possibly have deviation with the outdoor environment, so that the indoor simulation experiment has certain limitation and cannot really represent the actual field situation, and therefore, the establishment of the denitrification in-situ measurement device and method for the soil or sediment in the flooding environment has important scientific significance for the denitrification research of the soil or sediment in the flooding environment.
Disclosure of Invention
The technical problem to be solved is as follows: aiming at the technical problems, the invention provides a denitrification in-situ determination device and a denitrification in-situ determination method for soil or sediment in a flooded environment, which can establish a sealed environment in a field environment and avoid N in the atmosphere2The interference of (3) ensures that environmental conditions are consistent with the natural environment, and the sampling is lossless by pressing the piston, so that a tank body is not required to be damaged, the sealed environment can be maintained, the water sample collection can be accurately metered, the operation is simple and accurate, and the in-situ determination of the denitrification rate of the soil or the sediment in the flooding environment is finally realized.
The technical scheme is as follows: the utility model provides a soil or deposit denitrification normal position survey device under flooded environment, the device includes stainless steel handle, injection piston, PVC drum, stainless steel drum and PE transparent bottle, PVC drum and stainless steel drum are the inside hollow cylinder of both ends opening, and the PVC drum is transparent cylinder, and PVC drum surface is equipped with the scale, and PVC drum and stainless steel drum seamless intercommunication and PVC drum locate the top of stainless steel drum, the stainless steel handle is located PVC drum bottom outside for in pulling out stainless steel drum and inserting business turn over soil or deposit, injection piston swing joint is inside the PVC drum, and the injection piston includes piston body, PU pipe and valve switch, piston body head is the rubber buffer, and the afterbody is T shape catch bar, and the external diameter of rubber buffer equals the internal diameter of PVC drum, is used for sealed PVC drum, and T shape catch bar is used for drawing piston body, piston body bottom center is located to PU pipe one end, and the other end runs through and wears out piston body center top, and the other end that the piston body center top was worn out to the PU pipe is located to the valve switch, and the valve switch is used for the collection of water sample, passes through the silicone tube with the valve switch delivery port during the sampling and is connected with headspace sampling bottle, and the valve switch is opened during the sampling, keeps closing at ordinary times, ensures that the inside inclosed environment that is in of PVC drum, the transparent bottle of PE is top opening, the airtight and inside hollow ring form in bottom, the PVC drum outside is located to the transparent bottle cover of PE, and during the sampling, but the water sample is poured into in the ring, plays heat preservation and sealing effect, can prevent that water sample forms the bubble at PVC bobbin wall because air temperature is too high in the PVC.
Preferably, the PU pipe has an outer diameter of 4mm and an inner diameter of 3 mm.
Preferably, the PVC cylinder has an internal diameter of 70 mm.
Preferably, the inner diameter of the PE transparent bottle is 10 cm.
Preferably, the height of the PVC cylinder is not less than 30 cm.
The determination method based on the denitrification in-situ determination device for the soil or sediment in the flooded environment comprises the following steps:
implanting the bottom of the PVC cylinder and the stainless steel cylinder into soil through a stainless steel handle by a denitrification in-situ measuring device for soil or sediment in a flooded environment, so as to ensure that the soil is closely attached to the PVC cylinder and the stainless steel cylinder when an experiment is started, and a strict sealing environment is formed;
before sampling begins, firstly pulling out the injection piston, filling a water sample into the PVC cylinder, then slightly stirring clockwise for 30 seconds by using a glass rod to ensure that the water sample is uniform without interfering soil or sediments, opening a valve switch, vertically inserting the piston into the PVC cylinder to ensure that no air bubble is at the bottom of the piston, quickly closing the valve switch, and injecting the same water sample as that in the PVC cylinder into the upper part of the piston and the PE transparent plastic bottle;
standing for 4-6h to facilitate the denitrification in-situ determination of the solubility N in the soil or sediment in the flooded environment2The misce bene, when beginning the sampling, read the scale of PVC drum earlier, record volume V, then connect valve switch delivery port with the silica gel pipe, open the valve, press the injection piston with the hand simultaneously, water flows out through the PU pipe at piston center in the PVC drum, finally get into in the headspace sampling bottle through the silicone tube, treat that the water sample overflows and surpass sampling bottle volume 1.5 times, overflow the silicone tube, screw up the bottle lid rapidly, ensure that there is not the bubble in the bottle, preserve and survey aquatic dissolubility N with the good water sample 4 ℃2After the sample collection is finished, closing a valve switch, recording the volume delta V of the water sample discharged during the sampling according to the scales marked on the outer wall of the PVC cylinder, and after at least 2h, starting the second sampling, wherein the process is as above, so that the samples are continuously collected for 4-5 times, and the sampling is finished;
step four, the volume of the water sample discharged in each sampling is different, and the water solubility N determined in each sampling is determined according to the change of the volume2The concentration was calibrated by calibrating N using the following formula2Concentration CI：
Figure BDA0002351398490000021
In the formula, CIRepresents the solubility N of the water sample after the ith sampling and calibration2Concentration,. mu. mol. L-1；
CiRepresenting the solubility N of the water sample measured in the ith sampling2Concentration,. mu. mol. L-1；
ViThe volume of a water sample in the sampler is mL when the sample is sampled at the ith time;
k is n-1, n represents the number of samples;
V0the volume of a water sample in the sampler is mL when the first sampling is carried out;
Δ V represents the amount of water consumed per sample, mL;
step five, obtaining the calibrated concentration, and calculating N by using the following formula2Emission flux:
Figure BDA0002351398490000031
wherein F represents N2The discharge flux is mg.N.m-2·d-1；
Figure BDA0002351398490000032
Represents the solubility N in the sampler2Concentration variation, unit μmol. L-1·h-1；
V denotes the initial volume of the sampler and A denotes the base area of the sampler, therefore
Figure BDA0002351398490000033
The height of the sampler is h/cm;
24 means 24 hours/day;
28 represents N2The molar mass of (a);
10 denotes the volume to area conversion unit [10 ]3(conversion of L to m3)×10-2(cm to m)]。
Preferably, in the first step, the denitrification in-situ measuring device for soil or sediment in a flooded environment is used for implanting the bottom of the PVC cylinder and the stainless steel cylinder into the soil through a stainless steel handle at least 12 hours in advance.
Preferably, the water sample in the second step is field water, river water or culture solution.
Preferably, in the third step, the water solubility N is measured by a membrane sample introduction mass spectrometer2C, in the presence of a catalyst.
Has the advantages that: 1. the device can establish a sealed environment in a field environment, and avoids N in the atmosphere2The interference of (2) ensures that the environmental condition is consistent with the natural environment, the soil body is not needed to be damaged in the sampling process, and the sealed environment can be maintained.
2. The method provided by the invention is simple and accurate in water sample collection, and can realize in-situ determination of denitrification rate of soil or sediment in a flooded environment.
3. The method avoids the situation that the indoor simulation experiment and the outdoor have deviation, realizes the in-situ accurate quantification of the denitrification rate of the soil or the sediment in the flooding environment, and compares the method with the nitrate disappearance method, NO3 -The N recovery rate is 34.81-37.01%.
Drawings
FIG. 1 is a schematic view of a sampling apparatus according to the present invention;
FIG. 2 is a graph showing the denitrification rate measurement of Updite in example 2;
FIG. 3 shows the solubility N in example 22A graph of changes over time;
FIG. 4 shows solubility N in example 32Increasing the schematic over time;
FIG. 5 shows NO in example 33 --N decreases over time.
The numerical designations in the drawings represent the following: 1. a stainless steel handle; 2. opening and closing a valve; 3. an injection piston; 4, PU pipe; a PVC cylinder; 6, PE transparent bottle; 7. a stainless steel cylinder.
Detailed Description
The invention is further described below with reference to the accompanying drawings and specific embodiments.
Example 1
The water sample in this example is river water.
The utility model provides a soil or deposit denitrification normal position survey device under flooded environment, see figure 1, the device includes stainless steel handle 1, injection piston 3, PVC drum 5, stainless steel drum 7 and PE transparent bottle 6, PVC drum 5 and stainless steel drum 7 are the inside hollow cylinder of both ends opening, and PVC drum 5 is transparent cylinder, and PVC drum 5 surface is equipped with the scale, and PVC drum 5 and stainless steel drum 7 seamless intercommunication and PVC drum 5 locate the top of stainless steel drum 7, stainless steel handle 1 locates PVC drum 5 bottom outside for in pulling out stainless steel drum 7 and insert business turn over soil or deposit, injection piston 3 swing joint is inside PVC drum 5, and injection piston 3 includes piston body, PU pipe 4 and valve switch 2, the piston body head is the rubber buffer, and the afterbody is T shape catch bar, and the external diameter of rubber buffer equals the internal diameter of PVC drum 5, the device is used for sealing a PVC cylinder 5, a T-shaped push rod is used for drawing a piston body, one end of a PU tube 4 is arranged at the center of the bottom of the piston body, the other end penetrates through and penetrates out of the top of the center of the piston body, a valve switch 2 is arranged at the other end of the PU tube 4 which penetrates out of the top of the center of the piston body, the valve switch 2 is used for collecting a water sample, a water outlet of the valve switch 2 is connected with a headspace sampling bottle through a silicone tube during sampling, the valve switch is opened during sampling and is kept closed at ordinary times, so that the interior of the, the PE transparent bottle 6 is an annular body with an opening at the top end, a closed bottom end and a hollow interior, the PE transparent bottle 6 is sleeved outside the PVC cylinder 5, when sampling is carried out, a water sample can be injected into the annular body to play roles of heat preservation and sealing, can prevent the water sample in the PVC cylinder 5 from forming bubbles on the wall of the PVC cylinder 5 due to overhigh air temperature and can play a role in sealing. The outer diameter of the PU pipe is 4mm, and the inner diameter is 3 mm. The PVC cylinder has an internal diameter of 70 mm. The inner diameter of the PE transparent bottle is 10 cm. The height of the PVC cylinder is 50 cm.
The determination method based on the denitrification in-situ determination device for the soil or sediment in the flooded environment comprises the following steps:
firstly, implanting the bottom of a PVC cylinder 5 and a stainless steel cylinder 7 into soil through a stainless steel handle 1 by a soil or sediment denitrification in-situ measuring device in a flooded environment at least 12 hours in advance, and ensuring that the soil is closely attached to the PVC cylinder 5 and the stainless steel cylinder 7 to form a strict sealed environment when an experiment is started;
before sampling begins, firstly pulling out the injection piston 3, filling a water sample into the PVC cylinder 5, then slightly stirring clockwise for 30 seconds by using a glass rod to ensure that the water sample is uniform without interfering soil or sediments, opening the valve switch 2, vertically inserting the injection piston 3 into the PVC cylinder 5 to ensure that no air bubble is at the bottom of the injection piston 3, quickly closing the valve switch 2, and injecting the same water sample as that in the PVC cylinder 5 into the upper part of the injection piston 3 and the PE transparent bottle 6;
standing for 4-6h to facilitate the denitrification in-situ determination of the solubility N in the soil or sediment in the flooded environment2The misce bene, when beginning the sampling, read PVC drum 5's scale earlier, record volume V, then connect valve switch 2 delivery ports with the silica gel pipe, open valve switch 2, press injection piston 3 with the hand simultaneously, water flows through PU pipe 4 at injection piston 3 center in the PVC drum 5, finally get into in the headspace sampling bottle through the silicone tube, treat that the water sample overflows and surpass sampling bottle volume 1.5 times, overflow the silicone tube, screw up the bottle lid rapidly, ensure bubble-free in the bottle, save the good water sample 4 ℃ and advance appearance mass spectrograph survey aquatic dissolubility N through the membrane2The concentration C, after the sample collection is finished, the valve switch 2 is closed, the volume delta V of the water sample discharged during the sampling is recorded according to the scale marked on the outer wall of the PVC cylinder 5, after at least 2h, the second sampling is started, the process is as above, the samples are continuously collected for 4-5 times, and the sampling is finished;
step four, the volume of the water sample discharged in each sampling is different, and the water solubility N determined in each sampling is determined according to the change of the volume2The concentration was calibrated by calibrating N using the following formula2Concentration CI：
Figure BDA0002351398490000051
In the formula, CIRepresents the solubility N of the water sample after the ith sampling and calibration2Concentration,. mu. mol. L-1；
CiRepresenting the solubility N of the water sample measured in the ith sampling2Concentration,. mu. mol. L-1；
Vi represents the volume of a water sample in the sampler in the ith sampling, mL;
k is n-1, n represents the number of samples;
V0the volume of a water sample in the sampler is mL when the first sampling is carried out;
Δ V represents the amount of water consumed per sample, mL;
step five, obtaining the calibrated concentration, and calculating N by using the following formula2Emission flux:
Figure BDA0002351398490000052
wherein F represents N2The discharge flux is mg.N.m-2·d-1；
Figure BDA0002351398490000053
Represents the solubility N in the sampler2Concentration variation, unit μmol. L-1·h-1；
V denotes the initial volume of the sampler and A denotes the base area of the sampler, therefore
Figure BDA0002351398490000054
The height of the sampler is h/cm;
24 means 24 hours/day;
28 represents N2The molar mass of (a);
10 denotes the volume to area conversion unit [10 ]3(conversion of L to m3)×10-2(cm to m)]。
Example 2
The difference from example 1 is that in this example, the denitrification rate of the Uyghur soil is measured by using an in-situ measuring device, and the water sample is surface water, NO3 -The content of-N is 5.6 mg.L-1Referring to FIG. 2, the in-situ measuring device is inserted into the rice field at a depth of 15cm, the height of the PVC cylinder is 15cm after the surface water is filled in the PVC cylinder, sampling is carried out according to the step 3, the sampling is carried out for 0, 4, 8, 12 and 24 hours respectively, and after the concentration calibration, the time is used as the abscissa and N is used as the N2As ordinate, obtain
Figure BDA0002351398490000061
As shown in fig. 3, a, b, c represent 3 repeats.
As shown in Table 1, solubility N2There is a very good correlation between the increase in solubility and time, indicating that the method works well.
TABLE 1 solubility N2Correlation with time
Figure BDA0002351398490000062
The denitrification rate was calculated and as shown in Table 2, the denitrification rate of the non-fertilized Udag soil was 05.42-9.13 mg.N.m-2·day-1The different sample times have large spatial variability (CV 25.3%);
TABLE 2 UK-grid soil denitrification Rate
Figure BDA0002351398490000063
Example 3
The denitrification rate of the ugly soil was measured using an in situ measuring device as in example 1 and compared with the nitrate disappearance method.
Inserting an in-situ measuring device into the rice field at a depth of 15cm, adding a water sample containing nitrate and NO into the in-situ measuring device3 --N concentration of 10 mg.L-1The samples were taken as described in example 1, at 0, 4, 8, 12, 24 hours and the solubility N was determined using a Membrane Injection Mass Spectrometer (MIMS)2Concentration, determination of NO in water sample by means of spectrophotometer3 --N concentration, calibrated for concentration, with time as abscissa and concentration as ordinate, to obtain N2(FIG. 4) and NO3 --N (fig. 5) as a function of time, a, b, c in fig. 4 and 5 representing 3 repetitions.
TABLE 3N2Comparison of results of direct assay with disappearance of nitrate
Figure BDA0002351398490000064
As shown in Table 3, disappeared NO3 --N has 34.81% -37.01% conversion to N2。

Claims (9)

1. The utility model provides a soil or deposit denitrification normal position survey device under waterflooding environment, a serial communication port, the device includes stainless steel handle, injection piston, PVC drum, stainless steel drum and PE transparent bottle, PVC drum and stainless steel drum are the inside hollow cylinder of both ends opening, and the PVC drum is transparent cylinder, and PVC drum surface is equipped with the scale, and PVC drum and stainless steel drum seamless intercommunication and PVC drum locate the top of stainless steel drum, the stainless steel handle is located PVC drum bottom outside for in pulling out stainless steel drum and inserting business turn over soil or deposit, injection piston swing joint is inside the PVC drum, and the injection piston includes piston body, PU pipe and valve switch, piston body head is the rubber buffer, and the afterbody is T shape catch bar, and the external diameter of rubber buffer equals the internal diameter of PVC drum for sealed PVC drum, T shape catch bar are used for drawing the piston body, piston body bottom center is located to PU pipe one end, and the other end runs through and wears out piston body center top, and the other end that the PU pipe wore out piston body center top is located to the valve switch, the transparent bottle of PE is top opening, the airtight and inside hollow ring form in bottom, the transparent bottle cover of PE locates the PVC drum outside.
2. The denitrification in-situ measurement device for soil or sediment in a flooded environment as claimed in claim 1, wherein the PU tube has an outer diameter of 4mm and an inner diameter of 3 mm.
3. The device for denitrification in situ measurement of soil or sediment in a flooded environment as claimed in claim 1 wherein the PVC cylinder has an internal diameter of 70 mm.
4. The apparatus of claim 1, wherein the PE transparent bottle has an inner diameter of 10 cm.
5. The apparatus of claim 1, wherein the PVC cylinder is not less than 30cm high.
6. The method for measuring the denitrification in-situ measuring device for the soil or sediment in the flooded environment as claimed in claim 1, wherein the method comprises the following steps:
implanting the bottom of the PVC cylinder and the stainless steel cylinder into soil through a stainless steel handle by a denitrification in-situ measuring device for soil or sediment in a flooded environment, so as to ensure that the soil is closely attached to the PVC cylinder and the stainless steel cylinder when an experiment is started, and a strict sealing environment is formed;
before sampling begins, firstly pulling out the injection piston, filling a water sample into the PVC cylinder, then slightly stirring clockwise for 30 seconds by using a glass rod to ensure that the water sample is uniform without interfering soil or sediments, opening a valve switch, vertically inserting the piston into the PVC cylinder to ensure that no air bubble is at the bottom of the piston, quickly closing the valve switch, and injecting the same water sample as that in the PVC cylinder into the upper part of the piston and the PE transparent plastic bottle;
standing for 4-6h to facilitate the denitrification in-situ determination of the solubility N in the soil or sediment in the flooded environment2The misce bene, when beginning the sampling, read the scale of PVC drum earlier, record volume V, then connect valve switch delivery port with the silica gel pipe, open the valve, press the injection piston with the hand simultaneously, water flows out through the PU pipe at piston center in the PVC drum, finally get into in the headspace sampling bottle through the silicone tube, treat that the water sample overflows and surpass sampling bottle volume 1.5 times, pull out the silicone tube gently, screw up the bottle lid rapidly, ensure that there is not the bubble in the bottle, preserve and survey the aquatic dissolubility N with the good water sample 4 ℃2After the sample collection is finished, closing a valve switch, recording the volume delta V of the water sample discharged during the sampling according to the scales marked on the outer wall of the PVC cylinder, and after at least 2h, starting the second sampling, wherein the process is as above, so that the samples are continuously collected for 4-5 times, and the sampling is finished;
step four, the volume of the water sample discharged in each sampling is different, and the water solubility N determined in each sampling is determined according to the change of the volume2The concentration was calibrated by calibrating N using the following formula2Concentration CI：
Figure FDA0002351398480000021
In the formula, CIRepresents the solubility N of the water sample after the ith sampling and calibration2Concentration,. mu. mol. L-1；
CiRepresenting the solubility N of the water sample measured in the ith sampling2Concentration,. mu. mol. L-1；
ViThe volume of a water sample in the sampler is mL when the sample is sampled at the ith time;
k is n-1, n represents the number of samples;
V0the volume of a water sample in the sampler is mL when the first sampling is carried out;
Δ V represents the amount of water consumed per sample, mL;
step five, obtaining the calibrated concentration, and calculating N by using the following formula2Emission flux:
Figure FDA0002351398480000022
wherein F represents N2The discharge flux is mg.N.m-2·d-1；
Figure FDA0002351398480000023
Represents the solubility N in the sampler2Concentration variation, unit μmol. L-1·h-1；
V denotes the initial volume of the sampler and A denotes the base area of the sampler, therefore
Figure FDA0002351398480000024
The height of the sampler is h/cm;
24 means 24 hours/day;
28 represents N2The molar mass of (a);
10 denotes the volume to area conversion unit [10 ]3(conversion of L to m3)×10-2(cm to m)]。
7. The method as claimed in claim 6, wherein the soil or sediment denitrification in-situ measuring device in the flooded environment is used for implanting the bottom of the PVC cylinder and the stainless steel cylinder into the soil through the stainless steel handle at least 12h earlier in the first step.
8. The method as claimed in claim 6, wherein the water sample in step two is field water, river water or culture solution.
9. The method for determining denitrification in-situ measurement device for soil or sediment in flooded environment as claimed in claim 6, wherein in the third step, the water solubility N is determined by a membrane injection mass spectrometer2C, in the presence of a catalyst.
CN201911416804.9A 2019-12-31 2019-12-31 Soil or sediment denitrification in-situ determination device and determination method in flooded environment Pending CN111044601A (en)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112945795A (en) * 2021-03-25 2021-06-11 中国科学院南京地理与湖泊研究所 Quantitative measuring and calculating method for in-situ denitrification rate of river
CN113063922A (en) * 2021-03-15 2021-07-02 中国科学院南京土壤研究所 Culture device for determining nitrate conversion of flooded system based on MIMS method and application

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113063922A (en) * 2021-03-15 2021-07-02 中国科学院南京土壤研究所 Culture device for determining nitrate conversion of flooded system based on MIMS method and application
CN112945795A (en) * 2021-03-25 2021-06-11 中国科学院南京地理与湖泊研究所 Quantitative measuring and calculating method for in-situ denitrification rate of river
CN112945795B (en) * 2021-03-25 2022-12-02 中国科学院南京地理与湖泊研究所 Quantitative measuring and calculating method for in-situ denitrification rate of river

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