CN103592238A - Serum ethanol content determination kit - Google Patents

Serum ethanol content determination kit Download PDF

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Publication number
CN103592238A
CN103592238A CN201310464606.6A CN201310464606A CN103592238A CN 103592238 A CN103592238 A CN 103592238A CN 201310464606 A CN201310464606 A CN 201310464606A CN 103592238 A CN103592238 A CN 103592238A
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CN
China
Prior art keywords
ethanol content
reagent
purified water
serum
content determination
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201310464606.6A
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Chinese (zh)
Inventor
石丽萍
夏春伟
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
SHAOXING SHENGKANG BIOLOGY TECHNOLOGY Co Ltd
Original Assignee
SHAOXING SHENGKANG BIOLOGY TECHNOLOGY Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by SHAOXING SHENGKANG BIOLOGY TECHNOLOGY Co Ltd filed Critical SHAOXING SHENGKANG BIOLOGY TECHNOLOGY Co Ltd
Priority to CN201310464606.6A priority Critical patent/CN103592238A/en
Publication of CN103592238A publication Critical patent/CN103592238A/en
Pending legal-status Critical Current

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Abstract

The invention discloses a serum ethanol content determination kit. The serum ethanol content determination kit comprises two independent liquid reagents R1 and R2 which are matched for use, wherein the liquid reagent R1 comprises 18-22g/L of nicotinamide adenine dinucleotide (NAD) and the balance of purified water according to concentrations, and the reagent R2 comprises 35.5-41.5mg/L of adipic dihydrazide (ADH), 11.9-12.3g/L of Tris-HCL buffer solution and the balance of purified water according to concentrations. The serum ethanol content determination kit meets the serum ethanol content determination requirements in laboratories.

Description

Serum ethanol content is measured kit
Technical field
The invention belongs to serum and detect reagent technical field, especially relevant with a kind of kit of measuring for serum ethanol content.
Background technology
The quantitative measurement of serum ethanol is to the anxious disease that takes a disease of diagnosis and differential diagnosis, and rescue ethylism is very important, to processing traffic accident and forensic identification, is important scientific basis, so clinical labororatory generally carries out the mensuration work to serum ethanol content at present.The assay method of ethanol content is more, has physical method as gas chromatography, density bottle method etc., and chemical method is as potassium dichromate colourimetry, Alcoholic oxidase etc., and the whole bag of tricks cuts both ways.
Summary of the invention
The object of the invention is exactly to adapt to the needs that clinical labororatory measures serum ethanol content, and a kind of mensuration kit for serum ethanol content is provided.
For this reason, the present invention is by the following technical solutions: serum ethanol content is measured kit, comprise R1 and two kinds of independent liquid reagents of R2 of being used in conjunction with, it is characterized in that: described each constituent concentration of R1 reagent is: nicotinamide adenine dinucleotide (NAD) 18~22g/L, all the other are purified water; Described each constituent concentration of R2 reagent is: adipic dihydrazide (ADH) 35.5~41.5mg/L, and Tris-HCL damping fluid 11.9~12.3g/L, all the other are purified water.
Preferably, described each constituent concentration of R1 reagent is: nicotinamide adenine dinucleotide (NAD) 20g/L, and all the other are purified water; Described each constituent concentration of R2 reagent is: adipic dihydrazide (ADH) 38.5mg/L, and Tris-HCL damping fluid 12.1g/L, all the other are purified water.
The present invention uses Lactate Oxidase method to measure serum ethanol content, according to " ethanol+NAD acetaldehyde+NADH " this reaction principle, the rate of change in the mensuration NADH of wavelength 340nm place per minute absorbance, calculates its content.During mensuration, select two test tubes respectively as standard pipe and sample hose, the calibration solution that adds the known concentration of alcohol of 20 microlitre in standard pipe, in sample hose, add 20 microlitre sample to be measured, then in standard pipe and sample hose, respectively add R1 reagent 200 microlitres of the present invention to mix, at 37 ℃ of temperature, hatch after 5 minutes, in standard pipe and sample hose, respectively add again R2 reagent 50 microlitres of the present invention to mix, at 37 ℃ of temperature, postpone half a minute, use automatic clinical chemistry analyzer when predominant wavelength 340nm, to measure the absorbance A of each pipe, serum ethanol content (ALC concentration) just can be calculated and be obtained by following formula: ALC concentration (nmol/L)=(sample hose absorbance A u/ standard pipe absorbance) * calibration solution ethanol solubility.
Use the present invention can reach following beneficial effect: can use automatic clinical chemistry analyzer directly to measure serum ethanol content, be applicable to the needs that clinical labororatory measures ethanol content.
Embodiment
Below in conjunction with embodiment, the present invention is described in detail.
Embodiment mono-:
Kit of the present invention comprises R1 and the two kinds of independent liquid reagents of R2 that are used in conjunction with, and each constituent concentration of R1 reagent is: nicotinamide adenine dinucleotide (NAD) 18g/L, and all the other are purified water; Each constituent concentration of R2 reagent is: adipic dihydrazide (ADH) 35.5mg/L, and Tris-HCL damping fluid 11.9g/L, all the other are purified water.
Use Roche P800 automatic clinical chemistry analyzer, assay method adopts rate method, predominant wavelength adopts 340nm, auxiliary wavelength 405 nm, calibration mode adopts linear, the Direction of Reaction forward rises, select two test tubes respectively as standard pipe and sample hose, in standard pipe, add 20 microlitre calibration solutions, in sample hose, add 20 microlitre sample to be measured, then in standard pipe and sample hose, respectively add R1 reagent 200 microlitres to mix, at 37 ℃ of temperature, hatch 5 minutes, and then in standard pipe and sample hose, respectively add R2 reagent 50 microlitres of the present invention to mix, at 37 ℃ of temperature, postpone half a minute, at Roche P800 automatic clinical chemistry analyzer, measure the absorbance A of each pipe, by following formula, automatically calculate serum ethanol content: serum ethanol content (ALC concentration) just can be calculated and be obtained by following formula: ALC concentration (nmol/L)=(sample hose absorbance A u/ standard pipe absorbance) * calibration solution ethanol solubility.
Embodiment bis-:
Kit of the present invention comprises R1 and the two kinds of independent liquid reagents of R2 that are used in conjunction with, and each constituent concentration of R1 reagent is: nicotinamide adenine dinucleotide (NAD) 20g/L, and all the other are purified water; Each constituent concentration of R2 reagent is: adipic dihydrazide (ADH) 38.5mg/L, and Tris-HCL damping fluid 12.1g/L, all the other are purified water.
Use Roche P800 automatic clinical chemistry analyzer, assay method adopts rate method, predominant wavelength adopts 340nm, auxiliary wavelength 405 nm, calibration mode adopts linear, the Direction of Reaction forward rises, select two test tubes respectively as standard pipe and sample hose, in standard pipe, add 20 microlitre calibration solutions, in sample hose, add 20 microlitre sample to be measured, then in standard pipe and sample hose, respectively add R1 reagent 200 microlitres to mix, at 37 ℃ of temperature, hatch 5 minutes, and then in standard pipe and sample hose, respectively add R2 reagent 50 microlitres of the present invention to mix, at 37 ℃ of temperature, postpone half a minute, at Roche P800 automatic clinical chemistry analyzer, measure the absorbance A of each pipe, by following formula, automatically calculate serum ethanol content: serum ethanol content (ALC concentration) just can be calculated and be obtained by following formula: ALC concentration (nmol/L)=(sample hose absorbance A u/ standard pipe absorbance) * calibration solution ethanol solubility.
Embodiment tri-:
Kit of the present invention comprises R1 and the two kinds of independent liquid reagents of R2 that are used in conjunction with, and each constituent concentration of R1 reagent is: nicotinamide adenine dinucleotide (NAD) 22g/L, and all the other are purified water; Each constituent concentration of R2 reagent is: adipic dihydrazide (ADH) 41.5mg/L, and Tris-HCL damping fluid 12.3g/L, all the other are purified water.
Use Roche P800 automatic clinical chemistry analyzer, assay method adopts rate method, predominant wavelength adopts 340nm, auxiliary wavelength 405 nm, calibration mode adopts linear, the Direction of Reaction forward rises, select two test tubes respectively as standard pipe and sample hose, in standard pipe, add 20 microlitre calibration solutions, in sample hose, add 20 microlitre sample to be measured, then in standard pipe and sample hose, respectively add R1 reagent 200 microlitres to mix, at 37 ℃ of temperature, hatch 5 minutes, and then in standard pipe and sample hose, respectively add R2 reagent 50 microlitres of the present invention to mix, at 37 ℃ of temperature, postpone half a minute, at Roche P800 automatic clinical chemistry analyzer, measure the absorbance A of each pipe, by following formula, automatically calculate serum ethanol content: serum ethanol content (ALC concentration) just can be calculated and be obtained by following formula: ALC concentration (nmol/L)=(sample hose absorbance A u/ standard pipe absorbance) * calibration solution ethanol solubility.

Claims (2)

1. serum ethanol content is measured kit, comprises R1 and two kinds of independent liquid reagents of R2 of being used in conjunction with, it is characterized in that: described each constituent concentration of R1 reagent is: nicotinamide adenine dinucleotide (NAD) 18~22g/L, and all the other are purified water; Described each constituent concentration of R2 reagent is: adipic dihydrazide (ADH) 35.5~41.5mg/L, and Tris-HCL damping fluid 11.9~12.3g/L, all the other are purified water.
2. serum ethanol content according to claim 1 is measured kit, it is characterized in that: described each constituent concentration of R1 reagent is: nicotinamide adenine dinucleotide (NAD) 20g/L, and all the other are purified water; Described each constituent concentration of R2 reagent is: adipic dihydrazide (ADH) 38.5mg/L, and Tris-HCL damping fluid 12.1g/L, all the other are purified water.
CN201310464606.6A 2013-10-08 2013-10-08 Serum ethanol content determination kit Pending CN103592238A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201310464606.6A CN103592238A (en) 2013-10-08 2013-10-08 Serum ethanol content determination kit

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201310464606.6A CN103592238A (en) 2013-10-08 2013-10-08 Serum ethanol content determination kit

Publications (1)

Publication Number Publication Date
CN103592238A true CN103592238A (en) 2014-02-19

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CN201310464606.6A Pending CN103592238A (en) 2013-10-08 2013-10-08 Serum ethanol content determination kit

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112485249A (en) * 2020-11-27 2021-03-12 无锡市疾病预防控制中心 Kit for visual rapid detection of ethanol content in disinfectant and detection method

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112485249A (en) * 2020-11-27 2021-03-12 无锡市疾病预防控制中心 Kit for visual rapid detection of ethanol content in disinfectant and detection method

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Application publication date: 20140219