CN207876315U - A kind of modified form HCV virus parting detecting reagent - Google Patents
A kind of modified form HCV virus parting detecting reagent Download PDFInfo
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- CN207876315U CN207876315U CN201820196175.8U CN201820196175U CN207876315U CN 207876315 U CN207876315 U CN 207876315U CN 201820196175 U CN201820196175 U CN 201820196175U CN 207876315 U CN207876315 U CN 207876315U
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- hcv
- box body
- groove body
- lid
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Abstract
The utility model discloses a kind of modified form HCV virus parting detecting reagents, including groove body, box body, lid and control box, the side of the box body is equipped with control box, and one end inside control box is equipped with accumulator, the other end inside the control box is equipped with microcontroller, the bottom end of the tray interior is provided with cooling piece, and the both ends of tray interior are both provided with heat block, the top of the tray interior is equipped with temperature sensor, the top of the box body is hinged with lid, the output end of the temperature sensor is electrically connected by the input terminal of conducting wire and microcontroller, and the output end of microcontroller is electrically connected by the input terminal of conducting wire and heat block and cooling piece.The utility model is by being equipped with heat block, cooling piece, temperature sensor and microcontroller so that convenient for carrying out Conservation to detection liquid according to the specific demand of detection liquid, ensures that the reagent examined is free of extraneous thermal effects.
Description
Technical field
The utility model is related to HCV detection device technology fields, specially a kind of modified form HCV virus typing detection reagent
Box.
Background technology
Hepatitis C is caused by Hepatitis C Virus (HCV) infects, mainly by blood, body fluid communication, according to world health
There are 1.7 hundred million people's HCV infections in tissue estimation, the whole world, and in China, healthy population HCV-Ab IgG positive rate is 0.7%~3.1%, about 3800
Ten thousand people, HCV is mainly by blood born, and also passability is propagated, and is showed outside the various livers of chronic hepatitis, rheumatoid arthritis, flat
Flat lichen, glomerulonephritis, mixed type cryoglobulinemia, malignant lymphoma, porphyria cutanea tarda, depressive disease
Shape, hepatitis C is widely paid close attention to as one of the common problem of global most serious, with the development of society, medical level
Progress, there is HCV virus parting detecting reagent, but kit in the market is mostly common carton, inside is equipped with surveying
Test solution agent, when testing the preservation of sample and mixed liquor the more demanding of temperature is generally required to take out mixed liquor
In addition it is placed in refrigerating chamber and preserves, be also required to transport using curling stone or insulating foam box bag on the rocks when transporting detection kit
It is defeated, it is inconvenient, while the detection liquid test tube in common kit does not have protective device, is easy breakage, causes the wave of resource
Take, in addition, detection reagent is easy by outside contamination, to influence testing result, therefore is badly in need of a kind of modified form HCV virus point
Type detection kit meets the needs of people.
Utility model content
The purpose of this utility model is to provide a kind of modified form HCV virus parting detecting reagents, to solve the above-mentioned back of the body
The problem of being proposed in scape technology.
To achieve the above object, the utility model provides the following technical solutions：A kind of modified form HCV virus parting detection examination
The side of agent box, including groove body, box body, lid and control box, the box body is equipped with control box, and one end inside control box
Accumulator is installed, the other end inside the control box is equipped with microcontroller, and the bottom end of the tray interior is provided with refrigeration
Piece, and the both ends of tray interior are both provided with heat block, the top of the tray interior is equipped with temperature sensor, the box body
Internal middle position is provided with groove body, and one end of groove body interior side is provided with RNA extracting solutions, the RNA extracting solutions
The groove body of side is internally provided with HCV reaction solution II, and the groove body of the sides HCV reaction solution II is internally provided with mixed enzyme
Liquid, the groove body of the mixed enzyme solution side are internally provided with UNG enzymes+Taq enzyme, the other end setting of the groove body interior side
There are HCV sequencing primers, and the top of HCV sequencing primers, UNG enzyme+Taq enzyme, mixed enzyme solution, HCV reaction solution II and RNA extracting solutions
End is both provided with pipe lid, and the other side inside the groove body is evenly arranged with sterile centrifugation tube, and groove body is far from HCV sequencing primers
The box body of side is internally provided with aseptic sampler, and the top of the box body is hinged with lid, the temperature sensor it is defeated
Outlet is electrically connected by the input terminal of conducting wire and microcontroller, and the output end of microcontroller passes through conducting wire and heat block and refrigeration
The input terminal of piece is electrically connected.
Preferably, the junction of the lid and box body is provided with rubber gasket.
Preferably, the HCV sequencing primers, UNG enzymes+Taq enzyme, mixed enzyme solution, HCV reaction solution II and RNA extracting solutions
Bottom end be both provided with sponge bed course.
Preferably, the lid is equipped with ultraviolet germicidal lamp close to the side of groove body.
Preferably, it is provided with waterproof layer on the outside of the box body.
Compared with prior art, the utility model has the beneficial effects that：The modified form HCV virus parting detecting reagent is logical
It crosses and heat block, cooling piece, temperature sensor and microcontroller is installed so that be convenient for the specific demand according to detection liquid to detection
Liquid carries out Conservation, ensures that the reagent examined is free of extraneous thermal effects, ensures that the result examined is correct, while device
By being equipped with ultraviolet germicidal lamp so that convenient for carrying out sterilizing to tray interior, avoid by outside contamination, pollution inspection
Test agent, the accuracy of destructive test result, while device in groove body inner homogeneous by being provided with sponge bed course so that is convenient for
It protects testing reagent bottle without damage, avoids extraneous collision so that reagent breakage, causes damages and waste, while device passes through peace
Equipped with lid so that convenient for ensureing the seal of device entirety, avoid dust bacterium or water body from entering, pollute testing reagent, break
It is bad examine as a result, device is provided with waterproof layer by the surface in box body simultaneously so that avoid in transportational process by water body
Pollution.
Description of the drawings
Fig. 1 is the structural profile illustration of the utility model；
Fig. 2 is the schematic top plan view of the utility model；
Fig. 3 is the schematic elevation view of the utility model；
Fig. 4 is the control box diagrammatic cross-section of the utility model.
In figure：1- aseptic samplers；2- heat blocks；3-RNA extracting solutions；4- groove bodies；5-HCV reaction solutions II；6- ultraviolet lights
Bactericidal lamp；7- mixed enzyme solutions；8-UNG enzymes+Taq enzyme；9- temperature sensors；10- pipe lids；11-HCV sequencing primers；12- freezes
Piece；13- sterile centrifugation tubes；14- sponge bed courses；15- box bodys；16- lids；17- control box；18- accumulators；19- microcontrollers.
Specific implementation mode
The following will be combined with the drawings in the embodiments of the present invention, carries out the technical scheme in the embodiment of the utility model
Clearly and completely describe, it is clear that the described embodiments are only a part of the embodiments of the utility model, rather than whole
Embodiment.Based on the embodiments of the present invention, those of ordinary skill in the art are without making creative work
The every other embodiment obtained, shall fall within the protection scope of the present invention.
- 4 are please referred to Fig.1, a kind of embodiment provided by the utility model：A kind of modified form HCV virus typing detection reagent
The side of box, including groove body 4, box body 15, lid 16 and control box 17, box body 15 is equipped with control box 17, the outside of box body 15
It is provided with waterproof layer, convenient for playing the role of waterproof, and one end inside control box 17 is equipped with accumulator 18, in control box 17
The other end in portion is equipped with microcontroller 19, and the model of microcontroller 19 can be ZYWYKJ, and the bottom end inside box body 15 is provided with refrigeration
Piece 12, and the both ends inside box body 15 are both provided with heat block 2, the top inside box body 15 is equipped with temperature sensor 9, temperature
The model of sensor 9 can be CWDZ11, and the middle position inside box body 15 is provided with groove body 4, and the one of 4 interior side of groove body
End is provided with RNA extracting solutions 3, and the groove body 4 of 3 side of RNA extracting solutions is internally provided with HCV reaction solution II5, and HCV reaction solutions
The groove body 4 of the sides II5 is internally provided with mixed enzyme solution 7, and the groove body 4 of 7 side of mixed enzyme solution is internally provided with UNG enzymes+Taq
Enzyme 8, the other end of 4 interior side of groove body are provided with HCV sequencing primers 11, and HCV sequencing primers 11, UNG enzyme+Taq enzyme 8, mixed
Synthase liquid 7, HCV reaction solution II5 and RNA extracting solution 3 top be both provided with pipe lid 10, HCV sequencing primers 11, UNG enzymes+
Taq enzyme 8, mixed enzyme solution 7, HCV reaction solution II5 and RNA extracting solution 3 bottom end be both provided with sponge bed course 14, convenient for protection
Testing reagent, the other side inside groove body 4 is evenly arranged with sterile centrifugation tube 13, and groove body 4 is far from 11 side of HCV sequencing primers
Box body 15 be internally provided with aseptic sampler 1, the top of box body 15 is hinged with lid 16, the connection of lid 16 and box body 15
Place is provided with rubber gasket, convenient for increasing device seal, avoids dust bacterium from entering, lid 16 is close to the side of groove body 4
Ultraviolet germicidal lamp 6 is installed, convenient for carrying out sterilizing to 15 inside of box body, the output end of temperature sensor 9 passes through conducting wire
It is electrically connected with the input terminal of microcontroller 19, and the output end of microcontroller 19 passes through conducting wire and heat block 2 and cooling piece 12
Input terminal is electrically connected.
Operation principle：In use, accumulator 17 provides power for device, aseptic sampler 1 is used to extract tester first
Venous blood is collected in sterile centrifugation tube 13, after placing 4 hours, takes upper serum to be transferred in another centrifugation sterile tube, later
RNA extracting solutions 3, lid upper tube cap 10 is added, 100 μ l chloroforms are added in shaken well, after oscillation, Aspirate supernatant to newly sterile
In centrifuge tube 13, after sequentially adding isopropanol, absolute ethyl alcohol and DEPC water dissolutions, piping and druming repeatedly makes RNA all dissolve, low speed
Centrifuge the several seconds, preserve on ice to be checked, prepare amplification agent later, by quantitative HCV reaction solutions I and mixed enzyme solution 7 be added it is sterile from
In heart pipe 13, while preparing another sterile centrifugation tube 13, it is added in quantitative HCV reaction solutions II5 and UNG enzymes+Taq enzyme 8
In, it is separately added into the RNA solution prepared before later, and be placed in PCR instrument, records the sequence of example reaction, reaction finishes
Afterwards, observe fluorescence quantitative PCR instrument on probe whether take-off, take-off is positive, takes the PCR product of positive, adds
After entering SAP mixtures, the HCV sequencing primers 11 in binding reagents box are sequenced on the Genetic Analyser of automation, if not
It capable of being sequenced on the same day, controllable cooling piece 12 freezes so that box body 15 is in -20 DEG C of preservations, after receiving sequencing result,
The sequence replicating measured pastes in the Viral typing window on NCBI (attentional selection HCV partings) point and determines, out most upper
The result in face is just serum HCV hypotypes, to realize the parting detection of HCV virus.
It is obvious to a person skilled in the art that the present invention is not limited to the details of the above exemplary embodiments, and
And without departing substantially from the spirit or essential attributes of the utility model, it can realize that this practicality is new in other specific forms
Type.Therefore, in all respects, the present embodiments are to be considered as illustrative and not restrictive, this practicality is new
The range of type is indicated by the appended claims rather than the foregoing description, it is intended that containing in the equivalent requirements of the claims will be fallen
All changes in justice and range are embraced therein.Any reference numeral in claim should not be considered as limitation
Involved claim.
Claims (5)
1. a kind of modified form HCV virus parting detecting reagent, including groove body (4), box body (15), lid (16) and control box
(17), it is characterised in that：The side of the box body (15) is equipped with control box (17), and one end installation that control box (17) is internal
There are accumulator (18), the control box (17) the internal other end that microcontroller (19), the internal bottom of the box body (15) are installed
End is provided with cooling piece (12), and the both ends of box body (15) inside are both provided with heat block (2), the internal top of the box body (15)
End is equipped with temperature sensor (9), and the internal middle position of the box body (15) is provided with groove body (4), and groove body (4) is internal
One end of side is provided with RNA extracting solutions (3), and the groove body (4) of RNA extracting solutions (3) side is internally provided with HCV reactions
Liquid II (5), and the groove body (4) of HCV reaction solutions II (5) side is internally provided with mixed enzyme solution (7), the mixed enzyme solution (7)
The groove body (4) of side is internally provided with UNG enzymes+Taq enzyme (8), and the other end of groove body (4) interior side is provided with HCV
Sequencing primer (11), and HCV sequencing primers (11), UNG enzymes+Taq enzyme (8), mixed enzyme solution (7), HCV reaction solutions II (5) and
The top of RNA extracting solutions (3) is both provided with pipe lid (10), and the internal other side of the groove body (4) is evenly arranged with sterile centrifugation
It manages (13), and box body (15) of the groove body (4) far from HCV sequencing primer (11) side is internally provided with aseptic sampler (1), institute
The top for stating box body (15) is hinged with lid (16), and the output end of the temperature sensor (9) passes through conducting wire and microcontroller (19)
Input terminal be electrically connected, and the output end of microcontroller (19) passes through the input of conducting wire and heat block (2) and cooling piece (12)
End is electrically connected.
2. a kind of modified form HCV virus parting detecting reagent according to claim 1, it is characterised in that：The lid
(16) junction of box body (15) is provided with rubber gasket.
3. a kind of modified form HCV virus parting detecting reagent according to claim 1, it is characterised in that：The HCV is surveyed
Sequence primer (11), UNG enzymes+Taq enzyme (8), mixed enzyme solution (7), the bottom end of HCV reaction solutions II (5) and RNA extracting solutions (3) are equal
It is provided with sponge bed course (14).
4. a kind of modified form HCV virus parting detecting reagent according to claim 1, it is characterised in that：The lid
(16) ultraviolet germicidal lamp (6) is installed close to the side of groove body (4).
5. a kind of modified form HCV virus parting detecting reagent according to claim 1, it is characterised in that：The box body
(15) waterproof layer is provided on the outside of.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820196175.8U CN207876315U (en) | 2018-02-05 | 2018-02-05 | A kind of modified form HCV virus parting detecting reagent |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820196175.8U CN207876315U (en) | 2018-02-05 | 2018-02-05 | A kind of modified form HCV virus parting detecting reagent |
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| Publication Number | Publication Date |
|---|---|
| CN207876315U true CN207876315U (en) | 2018-09-18 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201820196175.8U Active CN207876315U (en) | 2018-02-05 | 2018-02-05 | A kind of modified form HCV virus parting detecting reagent |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112485249A (en) * | 2020-11-27 | 2021-03-12 | 无锡市疾病预防控制中心 | Kit for visual rapid detection of ethanol content in disinfectant and detection method |
-
2018
- 2018-02-05 CN CN201820196175.8U patent/CN207876315U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112485249A (en) * | 2020-11-27 | 2021-03-12 | 无锡市疾病预防控制中心 | Kit for visual rapid detection of ethanol content in disinfectant and detection method |
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