CN104762235A - Helicobacter pylori transport culture medium as well as preparation method and application thereof - Google Patents
Helicobacter pylori transport culture medium as well as preparation method and application thereof Download PDFInfo
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- CN104762235A CN104762235A CN201510167773.3A CN201510167773A CN104762235A CN 104762235 A CN104762235 A CN 104762235A CN 201510167773 A CN201510167773 A CN 201510167773A CN 104762235 A CN104762235 A CN 104762235A
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- helicobacter pylori
- culture medium
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- transporting
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
- C12Q1/045—Culture media therefor
Abstract
The invention relates to the field of microbiology, and in particular relates to a helicobacter pylori transport culture medium as well as a preparation method and application thereof. The transport culture medium comprises agar and reducing agents and can be used for effectively ensuring a micro-aerophilic environment of helicobacter pylori, and thus the active state of a clinical sample can be stored as long as possible, the survival time of the clinical sample in a non-culture state can reach more than 48 hours, the long-distance transport of a specimen can be facilitated, and the transport culture medium is beneficial for hospitals and inspectors to flexibly arrange the specimen examination time. According to the helicobacter pylori transport culture medium disclosed by the invention, the active state of the sample can be stored for a long time, so that the helicobacter pylori transport culture medium has a very good promotion effect on subsequent culture, can prevent collected helicobacter pylori from being transformed into a non-culturable state, and ensures that the positive detection rate can be greatly improved; and compared with a method without using the transport culture medium in the past, the positive detection rate can be increased by not less than 40% by adopting the transport culture medium disclosed by the invention.
Description
Technical field
The present invention relates to microbiological art, be specifically related to a kind of helicobacter pylori transporting culture medium and preparation and application thereof.
Background technology
Hp (Helicobacter pylori, Hp) is the micro-aerobasilus of a kind of Grain-negative.Since Nobel's physiology of 2005 and Medicine winner Warren and Marshall isolate Hp the 1983 gastroscopic biopsy samples from chronic gastritis patient, be subject to showing great attention to of the numerous scholar of domestic and international medical circle.Hp infects in global distribution, Hp and some disease of upper digestive tract are closely connected, be the important paathogenic factor of chronic gastritis, gastro-duodenal ulcer, occur closely related with cancer of the stomach, gastric mucosa-associated lymphoid tissue lymphoma, the World Health Organization has been classified as a class virulence factor.
The clinical detection of present stage, mainly contain invasive and the large class of Noninvasive two, the former has microbial culture, Rapid urease test etc., and the latter has urine breathalyse, antibody test etc.Microbial culture is the gold standard of diagnosing helicobacter pylori.Helicobacter pylori stable growth needs to rely on micro-aerobic environment, secondly ambient relative humidity need be kept to reach more than 90% when cultivating, and the temperature of the most suitable growth is 37 DEG C, and pH value is neutral.Helicobacter pylori can not growth and breeding in physical environment, clinically also need for some time from collecting sample to cultivating really to detect, and be vital for the follow-up cultivation breeding of helicobacter pylori during this period of time, clinically often because Sample preservation is improper, even if adopt best substratum, positive rate also can be caused greatly to reduce.The domestic transport preservation condition to sample is studied also fewer at present, or perhaps attention rate is also not high.The sample just that the present invention solves from after gathering to the problem how effectively ensureing helicobacter pylori survival rate during this period of time of cultivating.
Summary of the invention
For defect existing in prior art, the object of the invention is to, a kind of helicobacter pylori transporting culture medium is provided, to preserve collected kind helicobacter pylori sample for a long time, improve the positive rate of helicobacter pylori.
Another object of the present invention is to, preparation method and the application thereof of described helicobacter pylori transporting culture medium is provided.
Another object of the present invention is, provides a kind of method improving the positive rate of helicobacter pylori.
The present invention is achieved by the following technical solutions:
The present invention, by adding reductive agent in agar, develops a kind of transporting culture medium that can provide applicable helicobacter pylori stable growth desired gas environment and nutritive ingredient.
A first aspect of the present invention provides a kind of helicobacter pylori transporting culture medium, contains in helicobacter pylori transporting culture medium described in every 1000ml: agar 3.0-8.0g; Xitix 5.0-15.0mg; Glucose 5.0-10.0g; Glycine 10.0-50.0mg; Dithiothreitol (DTT) 5.0-15.0mg; Ferrous ammonium sulphate 3.0-8.0mg; Sodium borohydride 3.0-8.0mg.
Preferably, contain in helicobacter pylori transporting culture medium described in every 1000ml: agar 5.0g.
Preferably, contain in helicobacter pylori transporting culture medium described in every 1000ml: agar 5.0g, glucose 7.0g, dithiothreitol (DTT) 10.0mg.
More preferably, contain in helicobacter pylori transporting culture medium described in every 1000ml: agar 5.0g; Xitix 10mg; Glucose 7.0g; Glycine 30.0mg; Dithiothreitol (DTT) 10.0mg; Ferrous ammonium sulphate 5.0mg; Sodium borohydride 5.0mg.
Second aspect present invention provides a kind of method preparing described helicobacter pylori transporting culture medium, comprises step: take each component by weight, is added in deionized water, dissolves, autoclaving, packing.
Third aspect present invention provides described helicobacter pylori transporting culture medium and is transporting the purposes in helicobacter pylori.
Preferably, described purposes is for be used for transporting helicobacter pylori sample to improve Helicobacter pylori recall rate by described helicobacter pylori transporting culture medium.
A fourth aspect of the present invention provides a kind of method of transporting helicobacter pylori sample, comprises step: the helicobacter pylori sample of collection is put into described helicobacter pylori transporting culture medium and transports.
A fifth aspect of the present invention additionally provides a kind of method improving Helicobacter pylori recall rate, comprises step: the helicobacter pylori sample of collection is put into aforementioned helicobacter pylori transporting culture medium and transport, then carries out follow-up cultivation detection.
Beneficial effect of the present invention is:
(1) helicobacter pylori transhipment substratum of the present invention preparation is simple, with low cost.
(2) helicobacter pylori transporting culture medium of the present invention is semisolid medium, and convenient transportation is portable.Overcome liquid substratum easily to contact with bottleneck in transportation, cause uncapping and close the defect that lid microbiological contamination probability improves greatly.And semisolid substratum of the present invention is due to its poor fluidity, just greatly reduce the generation of this microbiological contamination situation.
(3) reductive agent that adds of the present invention, effectively can ensure the micro-aerobic environment of the one of helicobacter pylori, thus the active condition of clinical sample can be preserved as far as possible for a long time, the time of surviving under non-cultivation conditions can reach more than 48h, be conducive to sample to transport at a distance, be beneficial to the proving time of the flexible arrangement sample of hospital and reviewer.
(4) helicobacter pylori transporting culture medium of the present invention is owing to can preserve the active condition of sample for a long time, thus good promoter action is had to follow-up cultivation, the helicobacter pylori collected can be avoided to become non-state of cultivating state, greatly improve positive rate.Compared with the transhipment substratum in the past do not adopted, adopt transhipment substratum of the present invention, positive rate improves more than 40%.
Accompanying drawing explanation
Fig. 1: be the transhipment substratum schematic diagram of semi-solid of the present invention.
Fig. 2: for the embodiment of the present invention 5 is sampled idiographic flow schematic diagram.
Embodiment
Before further describing the specific embodiment of the invention, should be understood that protection scope of the present invention is not limited to following specific specific embodiments; It is also understood that the term used in the embodiment of the present invention is to describe specific specific embodiments, instead of in order to limit the scope of the invention.
When embodiment provides numerical range, should be understood that except non-invention is otherwise noted, between two end points of each numerical range and two end points, any one numerical value all can be selected.Unless otherwise defined, the same meaning that all technology used in the present invention and scientific terminology and those skilled in the art of the present technique understand usually.Except the concrete grammar used in embodiment, equipment, material, according to those skilled in the art to the grasp of prior art and record of the present invention, any method of prior art that is similar with the method described in the embodiment of the present invention, equipment, material or that be equal to, equipment and material can also be used to realize the present invention.
Unless otherwise indicated, disclosed in the present invention experimental technique, detection method, preparation method all adopt the routine techniques of the molecular biology of the art routine, biological chemistry, chromatin Structure and analysis, analytical chemistry, cell cultures, recombinant DNA technology and association area.These technology are existing in existing document improves explanation, specifically can see the MOLECULAR CLONING:A LABORATORY MANUAL such as Sambrook, Second edition, Cold Spring HarborLaboratory Press, 1989and Third edition, 2001; Ausubel etc., CURRENT PROTOCOLS INMOLECULAR BIOLOGY, John Wiley & Sons, New York, 1987and periodic updates; The seriesMETHODS IN ENZYMOLOGY, Academic Press, San Diego; Wolffe, CHROMATINSTRUCTURE AND FUNCTION, Third edition, Academic Press, San Diego, 1998; METHODSIN ENZYMOLOGY, Vol.304, Chromatin (P.M.Wassarman and A.P.Wolffe, eds.), AcademicPress, San Diego, 1999; With METHODS IN MOLECULAR BIOLOGY, Vol.119, ChromatinProtocols (P.B.Becker, ed.) Humana Press, Totowa, 1999 etc.
Embodiment 1
Transporting culture medium compound method of the present invention is, according to the form below component weighs:
Add in 1L deionized water, dissolve latter 121 DEG C, autoclaving 15min, be then sub-packed in each test tube, the stopping property of inspection test tube.
Embodiment 2
Transporting culture medium compound method of the present invention is, according to the form below component weighs:
Add in 1L deionized water, dissolve latter 121 DEG C, autoclaving 15min, be then sub-packed in each test tube, the stopping property of inspection test tube.
Embodiment 3
Transporting culture medium compound method of the present invention is, according to the form below component weighs:
Add in 1L deionized water, dissolve latter 121 DEG C, autoclaving 15min, be then sub-packed in each test tube, the stopping property of inspection test tube.
Embodiment 4
Transhipment seal of tube performance verification: the transhipment substratum 2 ~ 8 DEG C configured is placed two months, observes the change of its color, as being still blue, then can use, sealing property the result is as shown in table 1 below:
Table 1 container tightness test card
Note: the priming color of discolour silica gel is blue, when there being external moisture to enter container, the pink that can fade in various degree because water sucting degree is different
The transporting culture medium prepared by transhipment pipe packing embodiment 1 ~ 3 that the present invention adopts performance test qualified.
Embodiment 5
The transporting culture medium using the embodiment of the present invention 1 ~ 3 to prepare preserves the helicobacter pylori sample of clinical new collection.
Sampling flow process: HP transports pipe (2 ~ 8 DEG C of preservations), with the most advanced and sophisticated picking sample tissue of sampling rod after sample collection, 1/3 place (as Fig. 1) bottom test tube will be inserted with sampling tissue one end after confirming tissue apposition, then be transported to inspection laboratory to test, concrete operations flow process as shown in Figure 2.
After detecting, statistics, as shown in table 2 below:
Table 2 adopts embodiment 1 ~ 3 to transport substratum whether yin and yang attribute statistics
From the above results, the reductive agent that the present invention adds, effectively can ensure the micro-aerobic environment of the one of helicobacter pylori, thus the active condition of clinical sample can be preserved as far as possible for a long time, the time of surviving under non-cultivation conditions can reach more than 48h, be conducive to sample to transport at a distance, be beneficial to the proving time of the flexible arrangement sample of hospital and reviewer.
Helicobacter pylori transporting culture medium of the present invention, owing to can preserve the active condition of sample for a long time, thus has good promoter action to follow-up cultivation, the helicobacter pylori collected can be avoided to become non-state of cultivating state, greatly improve positive rate.Compared with the transhipment substratum in the past do not adopted, adopt transhipment substratum of the present invention, positive rate improves more than 40%.
The above; be only preferred embodiment of the present invention; not to any formal and substantial restriction of the present invention; should be understood that; for those skilled in the art; under the prerequisite not departing from the inventive method, also can make some improvement and supplement, these improve and supplement and also should be considered as protection scope of the present invention.All those skilled in the art, without departing from the spirit and scope of the present invention, a little change made when utilizing disclosed above technology contents, the equivalent variations of modifying and developing, be Equivalent embodiments of the present invention; Meanwhile, all according to substantial technological of the present invention to the change of any equivalent variations that above-described embodiment is done, modify and differentiation, all still belong in the scope of technical scheme of the present invention.
Claims (9)
1. a helicobacter pylori transporting culture medium, contains in helicobacter pylori transporting culture medium described in every 1000ml: agar 3.0-8.0g; Xitix 5.0-15.0mg; Glucose 5.0-10.0g; Glycine 10.0-50.0mg; Dithiothreitol (DTT) 5.0-15.0mg; Ferrous ammonium sulphate 3.0-8.0mg; Sodium borohydride 3.0-8.0mg.
2. helicobacter pylori transporting culture medium according to claim 1, is characterized in that, contains in helicobacter pylori transporting culture medium described in every 1000ml: agar 5.0g.
3. helicobacter pylori transporting culture medium according to claim 1, is characterized in that, contains in helicobacter pylori transporting culture medium described in every 1000ml: agar 5.0g, glucose 7.0g, dithiothreitol (DTT) 10.0mg.
4. helicobacter pylori transporting culture medium according to claim 1, is characterized in that, contains in helicobacter pylori transporting culture medium described in every 1000ml: agar 5.0g; Xitix 10.0mg; Glucose 7.0g; Glycine 30.0mg; Dithiothreitol (DTT) 10.0mg; Ferrous ammonium sulphate 5.0mg; Sodium borohydride 5.0mg.
5. prepare a method for helicobacter pylori transporting culture medium as described in claim as arbitrary in Claims 1 to 4, comprise step: take each component by weight, be added in deionized water, dissolve, autoclaving, packing.
6. helicobacter pylori transporting culture medium as described in claim as arbitrary in Claims 1 to 4 is transporting the purposes in helicobacter pylori sample.
7. purposes according to claim 6, is characterized in that, described purposes is for be used for transporting helicobacter pylori sample to improve Helicobacter pylori recall rate by described helicobacter pylori transporting culture medium.
8. transport a method for helicobacter pylori sample, comprise step: the helicobacter pylori sample of collection is put into helicobacter pylori transporting culture medium as described in claim as arbitrary in Claims 1 to 4 and transport.
9. improve a method for Helicobacter pylori recall rate, comprise step: the helicobacter pylori sample of collection is first put into helicobacter pylori transporting culture medium as described in claim as arbitrary in Claims 1 to 4 and transport, then carry out follow-up cultivation detection.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201510167773.3A CN104762235B (en) | 2015-04-07 | 2015-04-10 | A kind of helicobacter pylori transporting culture medium and its preparation and application |
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| CN2015101615192 | 2015-04-07 | ||
| CN201510161519 | 2015-04-07 | ||
| CN201510167773.3A CN104762235B (en) | 2015-04-07 | 2015-04-10 | A kind of helicobacter pylori transporting culture medium and its preparation and application |
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| CN104762235A true CN104762235A (en) | 2015-07-08 |
| CN104762235B CN104762235B (en) | 2018-11-09 |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105820975A (en) * | 2016-03-25 | 2016-08-03 | 美利泰格诊断试剂(嘉兴)有限公司 | Oral cavity helicobacter pylori culture method |
| CN106635862A (en) * | 2015-10-30 | 2017-05-10 | 杭州致远医学检验所有限公司 | Isolation medium for helicobacter pylori |
| CN108300680A (en) * | 2018-04-18 | 2018-07-20 | 深圳市伯劳特生物制品有限公司 | Helicobacter pylori transports culture medium |
| EP3786283A1 (en) * | 2015-12-18 | 2021-03-03 | Kanto Kagaku Kabushiki Kaisha | Long-term storage medium for culturing obligate anaerobic bacteria or microaerobic bacteria under aerobic environment, and method of detecting obligate anaerobic bacteria or microaerobic bacteria using said medium |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH08126495A (en) * | 1994-10-31 | 1996-05-21 | Intetsu Kobayashi | Medium for transportation of microorganism |
| CN1139544A (en) * | 1995-07-06 | 1997-01-08 | 中国预防医学科学院流行病学微生物研究所 | Quick diagnose reagent box for pylorus helicobacterium infection |
| WO2007047679A2 (en) * | 2005-10-14 | 2007-04-26 | The Research Foundation Of State Of University Of New York | Method for storage of clinical samples prior to culture |
| CN101048181A (en) * | 2004-08-13 | 2007-10-03 | 巴里·J·马沙尔 | Bacteria delivering system |
| CN102888442A (en) * | 2011-07-20 | 2013-01-23 | 农高惠 | Kit for rapid culture, identification and drug sensitivity of gastric helicobacter pylori and inspection method thereof |
| WO2014019696A1 (en) * | 2012-08-02 | 2014-02-06 | Università degli Studi "G. D'Annunzio" Chieti-Pescara | Transport medium for isolation and identification of helicobacter pylori. |
-
2015
- 2015-04-10 CN CN201510167773.3A patent/CN104762235B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH08126495A (en) * | 1994-10-31 | 1996-05-21 | Intetsu Kobayashi | Medium for transportation of microorganism |
| CN1139544A (en) * | 1995-07-06 | 1997-01-08 | 中国预防医学科学院流行病学微生物研究所 | Quick diagnose reagent box for pylorus helicobacterium infection |
| CN101048181A (en) * | 2004-08-13 | 2007-10-03 | 巴里·J·马沙尔 | Bacteria delivering system |
| WO2007047679A2 (en) * | 2005-10-14 | 2007-04-26 | The Research Foundation Of State Of University Of New York | Method for storage of clinical samples prior to culture |
| CN102888442A (en) * | 2011-07-20 | 2013-01-23 | 农高惠 | Kit for rapid culture, identification and drug sensitivity of gastric helicobacter pylori and inspection method thereof |
| WO2014019696A1 (en) * | 2012-08-02 | 2014-02-06 | Università degli Studi "G. D'Annunzio" Chieti-Pescara | Transport medium for isolation and identification of helicobacter pylori. |
Non-Patent Citations (2)
| Title |
|---|
| 何利华 等: "标本运输状态对幽门螺杆菌检出率的影响", 《中国病原生物学杂志》 * |
| 刘志国等 主编: "《中国幽门螺杆菌研究》", 30 April 1997, 科学技术文献出版社 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106635862A (en) * | 2015-10-30 | 2017-05-10 | 杭州致远医学检验所有限公司 | Isolation medium for helicobacter pylori |
| EP3786283A1 (en) * | 2015-12-18 | 2021-03-03 | Kanto Kagaku Kabushiki Kaisha | Long-term storage medium for culturing obligate anaerobic bacteria or microaerobic bacteria under aerobic environment, and method of detecting obligate anaerobic bacteria or microaerobic bacteria using said medium |
| CN105820975A (en) * | 2016-03-25 | 2016-08-03 | 美利泰格诊断试剂(嘉兴)有限公司 | Oral cavity helicobacter pylori culture method |
| CN108300680A (en) * | 2018-04-18 | 2018-07-20 | 深圳市伯劳特生物制品有限公司 | Helicobacter pylori transports culture medium |
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| CN104762235B (en) | 2018-11-09 |
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