CN112471162B

CN112471162B - Sterilization composition containing tetramycin and pyraclostrobin

Info

Publication number: CN112471162B
Application number: CN202011386034.0A
Authority: CN
Inventors: 隋书婷; 葛家成; 杨志鹏; 吕文东
Original assignee: Qingdao Hailier Biotechnology Co ltd
Current assignee: Qingdao Hailier Biotechnology Co ltd
Priority date: 2020-12-02
Filing date: 2020-12-02
Publication date: 2023-06-30
Anticipated expiration: 2040-12-02
Also published as: CN112471162A

Abstract

The invention relates to a sterilization composition containing tetramycin and pyraclostrobin, wherein the effective components comprise the tetramycin and the pyraclostrobin, and the mass ratio of the effective components of the tetramycin to the pyraclostrobin is 1:50-50:1. The bactericidal composition has good control effect on wheat powdery mildew, reduces the dosage of pesticides, reduces the adverse effect of the pesticides on the ecological environment, has important significance on comprehensive treatment of crop disease resistance, and has great economic and social benefits.

Description

Sterilization composition containing tetramycin and pyraclostrobin

Technical Field

The invention belongs to the technical field of agricultural plant protection, in particular relates to a bactericide composition capable of reducing the influence of pesticides on ecological environment, and particularly relates to a bactericide composition containing tetramycin and pyraclostrobin and application thereof.

Background

Tetramycin is a trade name of tetramycin, which has the English name tetramycin and CAS number 11076-50-9, and comprises four components A1, A2, B and C, wherein A1 and A2 are tetraene antibiotics of macrolide type, and the molecular formula is C ₃₅ H ₅₃ NO ₁₃ (A1)、C ₃₅ H ₅₃ NO ₁₄ (A2) The structural formula is shown as follows; b is peptide pyrimidine nucleotide antibiotics; c is a nitrogen-containing heterocyclic aromatic antibiotic. The structural formula is as follows:

tetramycin formulations contain a variety of antibiotics, wherein the peptide pyrimidine nucleotide antibiotics are used for preventing and controlling fungal diseases; the nitrogen-containing heterocyclic aromatic derivative antibiotics have the effect of improving the immunity of crops. Tetramycin has systemic antibacterial activity and prevents invasion and expansion of germs. The medicament forms a plurality of nutrient elements which can be absorbed and utilized by crops in the fermentation production process, has the functions of promoting healing and regeneration of the crop tissues after being subjected to trauma, enhancing the photosynthesis of plants and improving the yield; at the same time, the method can obviously promote the healing of the callus, promote the development of weak seedling root systems and recovery of aged root systems, improve the disease resistance of crops and optimize the quality of crops. Has wide bactericidal spectrum and extremely strong killing effect on twenty-six known pathogenic fungi of three major classes such as flagella, ascomycetes, fungi of semi-known mycotina and the like.

Pyraclostrobin has broad bactericidal spectrum, high bactericidal activity, protection and treatment activity on various plant diseases, and systemic conductivity; is used for preventing and treating wheat powdery mildew, cucumber downy mildew and cucumber gray mold. Chemical name: methyl 2- (1, 4-dimethyl-3-phenylpyrazol-5-yl) oxymethylphenyl methoxycarbamate, CAS accession number: 915410-70-7, the structural formula is as follows:

in recent years, with the increasingly single planting structure, the occurrence of wheat powdery mildew is more serious, and the traditional prevention and treatment measures and methods have difficult to control the wheat powdery mildew. The efficient broad-spectrum bactericide is adopted for mixing, so that the wheat powdery mildew can be well prevented and treated, the drug resistance of the wheat powdery mildew can be delayed, the pesticide application amount can be reduced, and the pesticide application period can be prolonged.

The composition of different action mechanisms is compounded, so that the method is very effective in delaying pathogen resistance, but in actual use, the composition of different action mechanisms is synergistic, additive and antagonistic, and the judgment needs to be made according to the actual application effect, in most cases, the compound effect of pesticides is antagonistic, the compound with the actual synergistic or additive effect is very few, and especially the compound with the very obvious synergistic effect and very high co-toxicity coefficient is very few. Through the screening of indoor activity and field efficacy, the inventor discovers that tetramycin and pyraclostrobin are mixed and have excellent indoor activity and field efficacy on wheat powdery mildew.

Disclosure of Invention

The invention aims to provide a bactericidal composition containing tetramycin and pyraclostrobin, which has the advantages of synergistic effect, low use cost and good control effect.

The technical scheme of the invention is as follows:

the active ingredients of the sterilization composition containing tetramycin and pyraclostrobin comprise active ingredients of tetramycin and pyraclostrobin;

further, the mass ratio of the tetramycin to the pyraclostrobin serving as the active ingredients of the bactericidal composition is 1:50-50:1;

further, the mass ratio of the tetramycin to the pyraclostrobin serving as the active ingredients of the bactericidal composition is 1:30-30:1;

further, the mass ratio of the tetramycin to the pyraclostrobin serving as the active ingredients of the bactericidal composition is 1:30-10:1;

further, the mass ratio of the tetramycin to the pyraclostrobin serving as the active ingredients of the bactericidal composition is 1:10-3:1.

The bactericidal composition containing tetramycin and pyraclostrobin accounts for 1-80%, preferably 1-50% of the total mass of the effective components of tetramycin and pyraclostrobin;

further, the bactericidal composition can be prepared into a preparation formulation which is allowed to be used in agriculture from an active ingredient and an auxiliary ingredient of a pesticide preparation;

further, the auxiliary components of the pesticide preparation comprise a carrier and an auxiliary agent;

further, the carrier is any one or more of water, solvent or filler, and the water is preferably deionized water;

further, the solvent is selected from one or more of N, N-dimethylformamide, cyclohexanone, toluene, xylene, dimethyl sulfoxide, methanol, ethanol, trimethylcyclohexanone, N-octyl pyrrolidone, ethanolamine, triethanolamine, isopropylamine, N-methylpyrrolidone, propanol, butanol, ethylene glycol, diethylene glycol, ethylene glycol methyl ether, butyl ether, ethanolamine, isopropylamine, ethyl acetate or acetonitrile;

further, the filler is selected from one or a mixture of more of kaolin, diatomite, bentonite, attapulgite, white carbon black, starch or light calcium carbonate;

further, the auxiliary agent at least comprises a surfactant, and other functional auxiliary agents such as an antifreezing agent, a thickening agent, a stabilizing agent, a disintegrating agent, a defoaming agent and the like can be added according to different use occasions and requirements;

further, the surfactant is selected from one or more of an emulsifier, a dispersant, a wetting agent or a penetrating agent;

further, the other functional auxiliary agents are selected from one or more of antifreezing agents, thickening agents, stabilizing agents, disintegrating agents or defoaming agents;

further, the emulsifier is selected from one or more of nonylphenol 500# (calcium alkylbenzenesulfonate), OP series phosphate (nonylphenol polyoxyethylene ether phosphate), 600# phosphate (phenylphenol polyoxyethylene ether phosphate), styrene polyoxyethylene ether ammonium sulfate, magnesium alkyldiphenyl ether disulfonate, triethanolamine salt, nonylphenol 400# (benzyl dimethyl phenol polyoxyethylene ether), nonylphenol 700# (alkylphenol formaldehyde resin polyoxyethylene ether), nonylphenol 36# (phenethyl phenol formaldehyde resin polyoxyethylene ether), nonylphenol 1600# (phenethyl phenol polyoxyethylene polypropylene ether), ethylene oxide-propylene oxide block copolymer, OP series (nonylphenol polyoxyethylene ether), BY series (castor oil polyoxyethylene ether), nonylphenol 33# (alkylaryl polyoxyethylene polyoxypropylene ether), span series (sorbitan monostearate) polyoxyethylene ether), tween series (sorbitan polyoxyethylene ether) or AEO series (fatty alcohol polyoxyethylene ether);

further, the dispersing agent is selected from one or more of polycarboxylate, lignosulfonate, alkylphenol ethoxylate formaldehyde condensate sulfate, calcium alkylbenzenesulfonate, naphthalene sulfonate formaldehyde condensate sodium salt, alkylphenol ethoxylate, fatty amine ethoxylate, fatty acid ethoxylate or glycerin fatty acid ester ethoxylate;

further, the wetting agent is selected from one or a mixture of more of sodium dodecyl sulfate, sodium dodecyl benzene sulfonate, nekal BX, wetting penetrating agent F, chinese honeylocust fruit powder, silkworm excrement or soapberry powder;

further, the penetrating agent is selected from one or a mixture of more of penetrating agent JFC (fatty alcohol polyoxyethylene ether), penetrating agent T (diisooctyl maleate sulfonate), azone or organic silicon;

further, the antifreezing agent is selected from a mixture consisting of one or more of ethylene glycol, propylene glycol, glycerol or urea;

further, the thickener is selected from one or more of xanthan gum, polyvinyl alcohol, bentonite, carboxymethyl cellulose or magnesium aluminum silicate;

further, the stabilizer is selected from one or a mixture of more of epoxidized soybean oil, epichlorohydrin, BHT, ethyl acetate and triphenyl phosphate;

further, the disintegrating agent is selected from one or a mixture of more of bentonite, urea, ammonium sulfate, aluminum chloride, low-substituted hydroxypropyl cellulose, lactose, citric acid, succinic acid or sodium bicarbonate;

further, the defoamer is selected from silicone oil, silicone compound, C ₁₀ ～C ₂₀ Saturated fatty acid compounds or C ₈ ～C ₁₀ A mixture of one or more fatty alcohol compounds;

all of the above materials are commercially available.

Further, the compositions may be formulated into pesticidally acceptable dosage forms, including solid, liquid, seed treatment, other formulations, according to methods well known to those skilled in the art;

further, the solid preparation is powder, dispersible tablet, granule, soluble powder, soluble granule, soluble tablet, emulsion powder, emulsion granule, water dispersible granule, wettable powder, microcapsule granule, powder, large granule and water dispersible tablet;

further, the liquid preparation is microcapsule suspending agent, dispersible agent, emulsifiable concentrate, oil emulsion, aqueous emulsion, microemulsion, dispersible oil suspending agent, oil dispersion powder, suspending agent, soluble agent, ultra-low volume liquid;

further, the seed treatment preparation is a suspension seed coating agent, a seed treatment dry powder agent, a seed treatment dispersible powder agent, a seed treatment suspending agent and a seed treatment liquid agent;

further, other formulations are baits, concentrated baits, gaseous formulations, aerosols, smoke formulations;

further, the preparation formulation is preferably wettable powder, water dispersible granules, suspending agents and aqueous emulsion;

further, the composition is prepared into wettable powder, and the components and the content of the composition are preferably as follows: tetramycin 0.1-10%, pyraclostrobin 0.1-80%, dispersant 2-10%, wetting agent 2-10%, and filler or carrier to 100%;

further, the composition is prepared into water dispersible granules, and the components and the content of the water dispersible granules are preferably as follows: tetramycin 0.1-10%, pyraclostrobin 0.1-80%, dispersant 3-12%, wetting agent 1-8%, disintegrating agent 1-10%, and filler or carrier to 100%;

further, the composition is prepared into a suspending agent, and the suspending agent comprises the following components in percentage by weight: tetramycin 0.1-10%, pyraclostrobin 0.1-80%, dispersant 2-10%, wetting agent 2-10%, defoamer 0.01-2%, thickener 0-2%, antifreeze 0-8% and deionized water to make up 100%;

further, the composition is prepared into an aqueous emulsion, and the components and the content of the composition are preferably as follows: tetramycin 0.1-10%, pyraclostrobin 0.1-80%, solvent 10-25%, emulsifying agent 5-15%, co-emulsion 1-4% and deionized water to 100%.

The invention has the advantages that:

1) The bactericidal composition has good synergy and lasting effect in a certain range, and the prevention effect is higher than that of a single agent;

2) The dosage of the pesticide is reduced, the residue of the pesticide on crops can be reduced, and the environmental pollution is reduced.

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention more concise, the present invention will be described in the following specific examples, but the present invention is by no means limited to these examples. The following examples are only preferred embodiments of the present invention, which can be used to describe the present invention, and should not be construed as limiting the scope of the invention. It should be noted that any modifications, equivalent substitutions and improvements made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Case of preparation

Example 1:21% tetramycin pyraclostrobin wettable powder (1:20)

The formula comprises the following components: 1% of tetramycin, 20% of pyraclostrobin, 4% of sodium lignin sulfonate, 3% of fatty alcohol polyoxyethylene ether, 6% of sodium dodecyl benzene sulfonate, 1% of sodium laurylsulfate and the balance of kaolin;

the preparation method comprises the following steps: the tetramycin, the pyraclostrobin, the dispersing agent, the wetting agent and the filler are uniformly mixed, uniformly mixed in a mixing tank, crushed by a jet mill and uniformly mixed again, and the wettable powder disclosed by the invention is prepared.

Example 2:31% tetramycin pyraclostrobin wettable powder (1:30)

The formula comprises the following components: tetramycin 1%, pyraclostrobin 30%, octyl phenol polyoxyethylene ether 2%, alkylphenol polyoxyethylene ether 3%, nekal BX3%, sodium dodecyl sulfate 2% and white carbon black make up the balance;

the preparation method comprises the following steps: as in example 1.

Example 3:33% tetramycin pyraclostrobin water dispersible granule (1:10)

The formula comprises the following components: tetramycin 3%, pyraclostrobin 30%, alkyl naphthalene sulfonate formaldehyde 2%, sodium lignin sulfonate 3%, sodium dodecyl sulfate 3%, sodium lauryl sulfate 2%, hydroxyethyl cellulose 1%, magnesium aluminum silicate 2%, sodium carbonate 2%, bentonite 2% and kaolin to make up the balance;

the preparation method comprises the following steps: the tetramycin, the pyraclostrobin, the dispersing agent, the wetting agent, the disintegrating agent and the filler are crushed together by air flow to obtain corresponding particle sizes, a semi-finished product is formed, and the semi-finished product is sent into a fluidized bed granulating dryer to be dried by a granulator, so that the water dispersible granule is prepared.

Example 4:3% tetramycin pyraclostrobin water dispersible granule (1:5)

The formula comprises the following components: tetramycin 0.5%, pyraclostrobin 2.5%, sodium lignin sulfonate 5%, sodium dodecyl sulfate 2.5%, urea 5%, white carbon black 5% and kaolin for the rest;

the preparation method comprises the following steps: same as in example 3.

Example 5:3% tetramycin pyraclostrobin suspension (1:5)

The formula comprises the following components: tetramycin 0.5%, pyraclostrobin 2.5%, polyoxyethylene polyoxypropylene ether 4%, sodium lignin sulfonate 1.5%, xanthan gum 0.25%, magnesium aluminum silicate 1.5%, sodium benzoate 1%, propylene glycol 3%, silicone oil 0.5%, and deionized water for the rest;

the preparation method comprises the following steps: according to the formula, water, a dispersing agent and a wetting agent are uniformly mixed, a defoaming agent and an anti-freezing agent are sequentially added under the stirring state, the mixture is uniformly sheared and mixed at a high speed, tetramycin and pyraclostrobin are added, the mixture is continuously sheared and uniformly mixed, and the mixture is ground in a horizontal sand mill, so that the particle size of the materials is totally below 5 mu m, a thickening agent is added, and the suspension is uniformly sheared, thus the suspension is prepared.

Example 6:5.5% tetramycin pyraclostrobin suspension (1:10)

The formula comprises the following components: tetramycin 0.5%, pyraclostrobin 5%, sodium polycarboxylate 3%, sodium lignin sulfonate 4%, sodium lauryl sulfate 3%, sodium dodecyl sulfate 2%, diethyl ether diglycol 4%, glycerol 2%, organosilicon 2%, silicone oil 1%, gelatin 1%, xanthan gum 3% and deionized water for the rest;

the preparation method comprises the following steps: same as in example 5.

Example 7:3.5% tetramycin pyraclostrobin aqueous emulsion (1:6)

The formula comprises the following components: tetramycin 0.5%, pyraclostrobin 3%, methyl oleate 2%, phenylphenol polyoxyethylene ether 3%, triethanolamine 3%, propylene glycol 3%, glycerol 3%, sodium acrylate 2%, sodium alginate 1%, organosilicon 3% and deionized water make up the balance;

the preparation method comprises the following steps: the tetramycin, the pyraclostrobin, the solvent, the emulsifier and other reagents are added together and uniformly mixed under high-speed stirring, so that the aqueous emulsion of the invention can be prepared.

Example 8:10% tetramycin pyraclostrobin aqueous emulsion (1:9)

The formula comprises the following components: tetramycin 1%, pyraclostrobin 9%, corn oil 3%, phenylphenol polyoxyethylene ether 2%, triethanolamine 3%, glycerol 3%, sodium acrylate 2%, diethyl ether diglycol 3%, polyvinyl alcohol 2% and deionized water for the balance;

the preparation method comprises the following steps: same as in example 7.

Indoor activity

Example 9: combined action of tetramycin and pyraclostrobin on wheat powdery mildew

The test is based on: with reference to the agricultural industry standards of the people's republic of China (NY/T1156.4-2006 and NY/T1156.6-2006): the test was carried out in part 4 (potted wheat powdery mildew control test) and part 6 (combined action test of compounding) of the "pesticide laboratory bioassay test criteria bactericide".

Test agent: 15% of tetramycin original medicine and 95% of pyraclostrobin original medicine are provided by a group development center.

Test target: powdery mildew (Erysiphe graminis).

Preparing a test material: and (5) selecting a disease-sensitive wheat variety (Lu Yuan) 502) for potting, and keeping the seedlings for standby after the seedlings grow to 2-3 leaf periods.

Drug configuration: the two crude drugs were dissolved in acetone, diluted with 0.1% tween 80 aqueous solution, and set to 5 series of mass concentrations.

And (3) medicament treatment: spraying the preparation onto wheat seedling, and naturally air drying. The test was run with no drug-containing treatment as a blank.

Inoculating and culturing: inoculating 24 hours after the medicament treatment, and uniformly shaking out fresh spores of powdery mildew generated in 24 hours on diseased wheat leaves to inoculate on treated 2-3-leaf stage potted wheat seedlings. 3 pots were treated per pot, 10 plants per pot. Then placing the mixture at the temperature of 20-24 ℃ and the humidity of 65-75%, and culturing the mixture under the condition that the illumination ratio is L and D=12:12 h.

Data investigation: grading investigation is carried out according to the disease condition of the blank control.

The following classification method is adopted:

level 0: no disease spots;

stage 1: the area of the disease spots accounts for less than 5% of the area of the whole leaf;

3 stages: the area of the disease spots accounts for 6% -15% of the area of the whole leaf;

5 stages: the area of the disease spots accounts for 16% -25% of the area of the whole leaf;

7 stages: the area of the disease spots accounts for 26% -50% of the area of the whole leaf;

stage 9: the area of the disease spots accounts for more than 50% of the area of the whole leaf.

Data statistics and analysis:

and calculating the disease index and the prevention and treatment effect of each treatment according to the investigation data.

The disease index is calculated according to the formula (1):

X＝[∑(Ni×i)/(N×9)]×100................................(1)

wherein:

x-disease index;

N _i -leaf numbers at each stage;

i—relative grade value;

n-total leaf number was investigated.

The control effect is calculated according to the formula (2):

P＝[(CK-PT)/CK]×100...................................... (2)

wherein:

p, the prevention and treatment effect, the unit is percentage;

CK-blank disease index;

PT-agent treatment index.

When the combined toxicity of the medicaments is measured, the synergy of the mixed medicaments is evaluated according to a grand cloud Pei co-toxicity coefficient method (CTC), namely CTC is less than or equal to 80 and is antagonistic, CTC is less than or equal to 120 and is additive, CTC is more than or equal to 120 and is synergistic, and the co-toxicity coefficient (CTC) is calculated according to the formula (3), the formula (4) and the formula (5).

ATI＝(S/M)×100.........................................(3)

Wherein:

ati—the measured virulence index of the mixture;

S-EC of Standard Agents ₅₀ Milligrams per liter (mg/L);

M-EC of mixture ₅₀ Units are milligrams per liter (mg/L).

TTI＝TI _A ×P _A +TI _B ×P _B .............................(4)

Wherein:

TTI-theoretical toxicity index of the mixture;

TI _A -a toxicity index of the agent;

P _A -the percentage of agent a in the mix, in percentage (%);

TI _B -toxicity index of B agent;

P _B the percentage content of the medicament B in the mixture is expressed as percentage (%).

CTC＝(ATI/TTI)×100.............................(5)

Wherein:

ctc—co-toxicity coefficient;

ati—actual measured virulence index of the mixture;

TTI-the theoretical toxicity index of the mixture.

Calculating test results by adopting DPS data processing software, and respectively solving virulence regression equations, EC of single test medicament and mixed medicaments with different proportions ₅₀ And EC (EC) ₉₀ And 95% confidence limit, and solving co-toxicity coefficients (CTC) of the two medicaments in different proportions, and screening out the optimal proportion of the test medicaments. Record raw data for each process all repetitions.

The test results are shown in Table 1:

table 1 indoor Activity test of tetramycin and pyraclostrobin against wheat powdery mildew

As can be seen from the indoor activity test (see Table 1), tetramycin and pyraclostrobin are mixed to obtain the wheat powdery mildew EC ₅₀ 5.0495mg/L and 0.6240mg/L respectively, wheat powdery mildew is sensitive to pyraclostrobin; tetramycin and pyraclostrobin are mixed in a ratio of 1:30, 1:10, 1:5, 1:3, 1:1, 3:1, 5:1, 10:1 and 30:1, and the co-toxicity coefficients are all greater than 120, so that the synergistic effect is shown for preventing and treating wheat powdery mildew.

Medicine effect in field

Example 10: tetramycin and pyraclostrobin mixed field efficacy for preventing and treating wheat powdery mildew

Test variety: a robusta 502.

Control object: powdery mildew of wheat.

Test site: the planting area of Tung Bai county in south China, henan province, the application time is 4 late days in 2020.

The test method comprises the following steps: with random block arrangement, cell area 25m ² 4 replicates. Fixed five-point sampling of each cell diagonal, each point looking up 0.25m ² Plants, the flag leaves of each plant were investigated after heading and the first leaf under the flag leaves. The wheat powdery mildew is applied by a conventional spray method at the initial stage of occurrence, the application is carried out once again for 9d after the application, the total application is carried out for 2 times, the condition of the wheat powdery mildew is investigated by fixed plants before the application, the disease index is recorded, the investigation is carried out once for 9d after the first application and 10d after the second application, and the prevention effects are calculated.

And (3) carrying out grading investigation, wherein grading standards refer to field efficacy test criteria GB/T17980.22-2000, and test data are analyzed by adopting a Duncan new complex polar difference method.

The 9-stage classification method is adopted:

powdery mildew classification method (in leaf units):

level 0: no disease spots;

3 stages: the area of the disease spots accounts for 6-15% of the area of the whole leaf;

5 stages: the area of the disease spots accounts for 16-25% of the area of the whole leaf;

7 stages: the area of the disease spots accounts for 26-50% of the area of the whole leaf;

The drug effect is calculated according to the formula (1) and the formula (2):

(1) Disease index = Σ100 [ (number of leaves at each stage×number of relative stages)/(total number of leaves in investigation×9) ] ×100

(2) Control effect (%) = [1- (pre-treatment area drug condition index x treatment area post-treatment area drug condition index)/(post-treatment area drug condition index x treatment area pre-treatment area drug condition index) ]x100

Safety investigation:

during the test, whether the crops have phytotoxicity symptoms or not is observed, and if the phytotoxicity is recorded, the phytotoxicity degree of the crops is accurately described, and in addition, the beneficial effects on the crops are recorded.

Table 2 field efficacy of tetramycin and pyraclostrobin for controlling wheat powdery mildew

Field efficacy display (see table 2): under the same environmental conditions, the overall prevention effect of each treatment field effect of 9 days after the first application of the pesticide for preventing and treating wheat powdery mildew is 60.64-89.15%, and 10g/hm of 5.5% tetramycin pyraclostrobin suspending agent is sprayed ² 、25g/hm ² 、40g/hm ² The control effect of the three different doses on wheat powdery mildew is 77.71%, 83.63% and 89.15%, which are superior or obviously superior to single dose and local conventional medicament (60% azoxystrobin water dispersible granule), wherein the dosage of 5.5% tetramycin pyraclostrobin suspending agent is 40g/hm ² The time-proof effect is best. The overall control effect of each treated field effect is 62.11-90.45% 10 days after the second pesticide application, and 10g/hm of tetramycin and pyraclostrobin suspending agent with 5.5% is sprayed ² 、25g/hm ² 、40g/hm ² The control of wheat powdery mildew after three different doses is 78.06%, 84.64% and 90.45% respectively.

The indoor test and the field test result show that the tetramycin and the pyraclostrobin have better control effect on the wheat powdery mildew when being compounded in a ratio of 1:10, the single-dose effect of the tetramycin and the pyraclostrobin on controlling the wheat powdery mildew is obviously lower than that of a compound medicament, and in the test process, the tetramycin and the pyraclostrobin are safe to the wheat at each medicament test concentration and have no phytotoxicity. Therefore, the tetramycin and the pyraclostrobin are compounded in a ratio of 1:10, so that the tendency of the rising of the resistance of the wheat powdery mildew year by year can be relieved, the control effect of the wheat powdery mildew is improved, and the service life of the medicament is prolonged.

Claims

1. The sterilization composition containing tetramycin and pyraclostrobin is characterized by comprising an active ingredient A and an active ingredient B, wherein the active ingredient A is tetramycin, and the active ingredient B is pyraclostrobin; the mass ratio of the effective component tetramycin to the effective component pyraclostrobin is 1:30-30:1.

2. The bactericidal composition of claim 1, wherein the mass ratio of the active ingredient tetramycin to the active ingredient pyraclostrobin is 1:30-10:1.

3. The bactericidal composition according to claim 2, wherein the mass ratio of the active ingredients tetramycin to pyraclostrobin is 1:10-3:1.

4. The bactericidal composition of claim 1, wherein the sum of the mass of the active ingredients tetramycin and pyraclostrobin is 1-80% of the composition.

5. The bactericidal composition according to claim 4, wherein the sum of the mass of the active ingredients tetramycin and pyraclostrobin is 1-50% of the composition.

6. The composition of claim 1, wherein the composition further comprises a pesticide adjuvant in addition to the active ingredient.

7. The bactericidal composition of claim 6, wherein the bactericidal composition is formulated in an agriculturally acceptable dosage form comprising any of a powder, dispersible tablet, granule, soluble powder, soluble granule, soluble tablet, emulsion, water dispersible granule, wettable powder, microencapsulated granule, macrogranule, microcapsule suspension, dispersible liquid, emulsifiable concentrate, oil emulsion, aqueous emulsion, microemulsion, dispersible oil suspension, oil-dispersible powder, suspension, suspoemulsion, soluble solution, ultra-low volume liquid, suspension seed coating, seed treatment dry powder, seed treatment dispersible powder, seed treatment suspension, seed treatment liquid.

8. The bactericidal composition of claim 7, wherein the formulation comprises any of wettable powder, water dispersible granules, suspending agents, and aqueous emulsions.

9. Use of the fungicidal composition according to any one of claims 1 to 8 for controlling diseases on crops.

10. The use according to claim 9, wherein the disease is wheat powdery mildew.