CN112471162A

CN112471162A - Bactericidal composition containing tetramycin and pyraclostrobin

Info

Publication number: CN112471162A
Application number: CN202011386034.0A
Authority: CN
Inventors: 隋书婷; 葛家成; 杨志鹏; 吕文东
Original assignee: Qingdao Hailier Biotechnology Co ltd
Current assignee: Qingdao Hailier Biotechnology Co ltd
Priority date: 2020-12-02
Filing date: 2020-12-02
Publication date: 2021-03-12
Anticipated expiration: 2040-12-02
Also published as: CN112471162B

Abstract

The invention relates to a bactericidal composition containing tetramycin and pyraclostrobin, which comprises the effective components of tetramycin and pyraclostrobin, wherein the mass ratio of the effective components of tetramycin to pyraclostrobin is 1: 50-50: 1. The bactericidal composition has good control effect on wheat powdery mildew, reduces the using dose of pesticides, reduces the adverse effect of the pesticides on the ecological environment, has important significance on the comprehensive treatment of crop disease resistance, and has great economic benefit and social benefit.

Description

Bactericidal composition containing tetramycin and pyraclostrobin

Technical Field

The invention belongs to the technical field of agricultural plant protection, and particularly relates to a bactericide composition capable of reducing the influence of pesticides on ecological environment, in particular to a bactericide composition containing tetramycin and pyraclostrobin and application thereof.

Background

Tetramycin is a compound named tetramycin by the name of British, CAS number 11076-50-9, and comprises four components of A1, A2, B and C, wherein A1 and A2 are macrolide tetraene antibiotics with the molecular formula of C₃₅H₅₃NO₁₃(A1)、C₃₅H₅₃NO₁₄(A2) The structural formula is shown as follows; b is a peptide pyrimidine nucleotide antibiotic; c is a nitrogen heterocyclic aromatic antibiotic. The structural formula is as follows:

the tetramycin preparation contains a plurality of antibiotics, wherein the peptide pyrimidine nucleotide antibiotics are used for preventing and treating fungal diseases; the azacyclo aromatic derivative-containing antibiotic has the function of improving the immunity of crops. Tetramycin has systemic bacteriostatic activity and can prevent invasion and expansion of pathogenic bacteria. In the process of fermentation production of the medicament, a plurality of nutrient elements capable of being absorbed and utilized by crops are formed, so that the healing and regeneration functions of the crop tissues after being subjected to trauma are promoted, the photosynthesis of plants is enhanced, and the yield is increased; meanwhile, the healing of callus can be obviously promoted, the development of weak seedling root systems and the recovery of aged root systems are promoted, the disease resistance of crops is improved, and the quality of the crops is optimized. The bactericidal spectrum is wide, and the bactericidal composition has extremely strong killing effect on twenty-six known pathogenic fungi such as flagellate fungi, ascomycetes and fungi imperfecti.

Pyraclostrobin (pyrametostatin), has broad bactericidal spectrum, high bactericidal activity, protective and therapeutic activity for various plant diseases, and systemic conductivity; can be used for preventing and treating wheat powdery mildew, cucumber downy mildew and cucumber gray mold. Chemical name: methyl 2- (1, 4-dimethyl-3-phenylpyrazol-5-yl) oxymethylphenyl methoxycarbamate, CAS accession No.: 915410-70-7, the structural formula is as follows:

in recent years, with the increasingly simple planting structure, wheat powdery mildew has become more serious, and the traditional control measures and methods have been difficult to control. The high-efficiency broad-spectrum bactericide is adopted for mixing, so that the wheat powdery mildew can be well prevented and treated, the drug resistance of the wheat powdery mildew can be delayed, the application amount of the pesticide is reduced, and the application period of the pesticide is prolonged.

The compounding of the components with different action mechanisms is an effective method for delaying the resistance of pathogenic bacteria, but the compounding of the components with different action mechanisms in actual use is synergistic, additive or antagonistic action, and needs to be judged according to the actual application effect. Through screening of indoor activity and field efficacy, the inventor finds that the mixing of tetramycin and pyraclostrobin has excellent indoor activity and field efficacy on wheat powdery mildew.

Disclosure of Invention

The invention aims to provide a bactericidal composition containing tetramycin and pyraclostrobin, which has a synergistic effect, low use cost and good control effect.

The technical scheme of the invention is as follows:

the active ingredients of the sterilization composition containing tetramycin and pyraclostrobin comprise active ingredients of tetramycin and active ingredients of pyraclostrobin;

further, the mass ratio of the tetramycin to the pyraclostrobin serving as the effective components of the bactericidal composition is 1: 50-50: 1;

further, the mass ratio of the tetramycin to the pyraclostrobin serving as the effective components of the bactericidal composition is 1: 30-30: 1;

further, the mass ratio of the tetramycin to the pyraclostrobin serving as the effective components of the bactericidal composition is 1: 30-10: 1;

further, the mass ratio of the tetramycin to the pyraclostrobin serving as the effective components of the bactericidal composition is 1: 10-3: 1.

The bactericidal composition contains tetramycin and pyraclostrobin, and the sum of the mass of the effective component tetramycin and the mass of the effective component pyraclostrobin accounts for 1% -80% of the composition, preferably 1% -50%;

furthermore, the bactericidal composition can be prepared into preparation formulations which are agriculturally allowed to be used by active ingredients and auxiliary ingredients of pesticide preparations;

further, the auxiliary components of the pesticide preparation comprise a carrier and an auxiliary agent;

further, the carrier is any one or more of water, a solvent or a filler, and the water is preferably deionized water;

further, the solvent is selected from one or a mixture of more of N, N-dimethylformamide, cyclohexanone, toluene, xylene, dimethyl sulfoxide, methanol, ethanol, trimethylcyclohexanone, N-octylpyrrolidone, ethanolamine, triethanolamine, isopropylamine, N-methylpyrrolidone, propanol, butanol, ethylene glycol, diethylene glycol, ethylene glycol methyl ether, butyl ether, ethanolamine, isopropylamine, ethyl acetate or acetonitrile;

further, the filler is selected from one or a mixture of more of kaolin, diatomite, bentonite, attapulgite, white carbon black, starch or light calcium carbonate;

furthermore, the auxiliary agent at least comprises a surfactant, and other functional auxiliary agents such as an antifreezing agent, a thickening agent, a stabilizing agent, a disintegrating agent, a defoaming agent and the like can be added according to different use occasions and requirements;

further, the surfactant is selected from one or more of an emulsifier, a dispersant, a wetting agent or a penetrant;

further, the other functional auxiliary agents are selected from one or more of an antifreezing agent, a thickening agent, a stabilizing agent, a disintegrating agent or a defoaming agent;

further, the emulsifier is selected from one or more of agricultural milk 500# (calcium alkyl benzene sulfonate), OP series phosphate ester (nonylphenol polyoxyethylene ether phosphate ester), 600# phosphate ester (phenylphenol polyoxyethylene ether phosphate ester), styrene polyoxyethylene ether ammonium sulfate salt, magnesium salt of alkyl diphenyl ether disulfonate, triethanolamine salt, agricultural milk 400# (benzyldimethylphenol polyoxyethylene ether), agricultural milk 700# (alkylphenol formaldehyde resin polyoxyethylene ether), Ningru 36# (phenethylphenol formaldehyde resin polyoxyethylene ether), agricultural milk 1600# (phenethylphenol polyoxyethylene polyoxypropylene ether), ethylene oxide-propylene oxide block copolymer, OP series (nonylphenol polyoxyethylene ether), BY series (castor oil polyoxyethylene ether), agricultural dehydrated milk 33# (alkylaryl polyoxyethylene polyoxypropylene ether), span series (sorbitan monostearate), Tween series (sorbitan fatty acid ester polyoxyethylene ether) or AEO series (fatty alcohol polyoxyethylene ether) A mixture of seed constituents;

further, the dispersing agent is selected from one or a mixture of a plurality of polycarboxylate, lignosulfonate, alkylphenol polyoxyethylene formaldehyde condensate sulfate, calcium alkylbenzene sulfonate, naphthalene sulfonic acid formaldehyde condensate sodium salt, alkylphenol polyoxyethylene, fatty amine polyoxyethylene, fatty acid polyoxyethylene or glycerol fatty acid ester polyoxyethylene;

further, the wetting agent is selected from one or a mixture of more of sodium dodecyl sulfate, sodium dodecyl benzene sulfonate, nekal BX, wetting penetrant F, Chinese honeylocust fruit powder, silkworm excrement or soapberry powder;

further, the penetrating agent is selected from a mixture consisting of one or more of penetrating agent JFC (fatty alcohol-polyoxyethylene ether), penetrating agent T (diisooctyl maleate sulfonate), azone or organic silicon;

further, the antifreezing agent is a mixture consisting of one or more of ethylene glycol, propylene glycol, glycerol or urea;

further, the thickening agent is selected from one or more of xanthan gum, polyvinyl alcohol, bentonite, carboxymethyl cellulose or magnesium aluminum silicate;

further, the stabilizer is selected from one or more of epoxidized soybean oil, epichlorohydrin, BHT, ethyl acetate and triphenyl phosphate;

further, the disintegrating agent is selected from one or more of bentonite, urea, ammonium sulfate, aluminum chloride, low-substituted hydroxypropyl cellulose, lactose, citric acid, succinic acid or sodium bicarbonate;

further, the defoaming agent is selected from silicone oil and silicone compounds、C₁₀～C₂₀Saturated fatty acids or C₈～C₁₀One or more of fatty alcohol compounds;

all of the above are commercially available.

Further, the composition may be formulated into a pesticidally acceptable formulation, including solid formulations, liquid formulations, seed treatment formulations, other formulations, according to methods well known to those skilled in the art;

further, the solid preparation is powder, dispersible tablets, granules, soluble powder, soluble granules, soluble tablets, milk powder, milk granules, water dispersible granules, wettable powder, micro-capsule granules, powder, macro-granules and water dispersible tablets;

further, the liquid preparation is microcapsule suspending agent, dispersible agent, missible oil, oil emulsion, aqueous emulsion, microemulsion, dispersible oil suspending agent, oil dispersion powder, suspending agent, suspoemulsion, soluble agent and ultra-low volume liquid;

further, the seed treatment preparation is a suspended seed coating agent, a seed treatment dry powder agent, a seed treatment dispersible powder agent, a seed treatment suspending agent and a seed treatment liquid agent;

further, the other preparations are bait, concentrated bait, gas preparation, aerosol, smoke agent;

furthermore, the preparation formulation is preferably wettable powder, water dispersible granules, suspending agents and aqueous emulsion;

further, the composition is prepared into wettable powder, and the components and the content of the wettable powder are preferably as follows: 0.1 to 10 percent of tetramycin, 0.1 to 80 percent of pyraclostrobin, 2 to 10 percent of dispersant, 2 to 10 percent of wetting agent and the balance of filler or carrier to 100 percent;

further, the composition is prepared into water dispersible granules, and the water dispersible granules preferably comprise the following components in percentage by weight: 0.1 to 10 percent of tetramycin, 0.1 to 80 percent of pyraclostrobin, 3 to 12 percent of dispersant, 1 to 8 percent of wetting agent, 1 to 10 percent of disintegrant and the balance of filler or carrier to 100 percent;

further, the composition is prepared into a suspending agent, and the components and the content of the suspending agent are preferably as follows: 0.1-10% of tetramycin, 0.1-80% of pyraclostrobin, 2-10% of dispersant, 2-10% of wetting agent, 0.01-2% of defoaming agent, 0-2% of thickening agent, 0-8% of antifreeze agent and deionized water for supplementing to 100%;

further, the composition is prepared into an aqueous emulsion, and the composition preferably comprises the following components in percentage by weight: 0.1-10% of tetramycin, 0.1-80% of pyraclostrobin, 10-25% of solvent, 5-15% of emulsifier, 1-4% of co-emulsion and deionized water, wherein the deionized water is used for supplementing to 100%.

The invention has the advantages that:

1) the bactericidal composition has good synergy and lasting effect within a certain range, and the control effect is higher than that of a single agent;

2) the dosage of the pesticide is reduced, the residue of the pesticide on crops can be reduced, and the environmental pollution is reduced.

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention more concise and clear, the present invention is described with the following specific embodiments, but the present invention is by no means limited to these embodiments. The following described examples are only preferred embodiments of the present invention, which can be used to describe the present invention and should not be construed as limiting the scope of the present invention. It should be understood that any modification, equivalent replacement, and improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Preparation of the preparation

Example 1: 21% tetramycin zolamide wettable powder (1:20)

The formula is as follows: 1% of tetramycin, 20% of pyraclostrobin, 4% of sodium lignin sulfonate, 3% of fatty alcohol polyoxyethylene ether, 6% of sodium dodecyl benzene sulfonate, 1% of sodium lauryl sulfate and the balance of kaolin;

the preparation method comprises the following steps: the tetramycin, the pyraclostrobin, the dispersant, the wetting agent and the filler are uniformly mixed in a mixing tank, are crushed by a jet mill and are uniformly mixed again to prepare the wettable powder.

Example 2: 31% tetramycin zolamide wettable powder (1:30)

The formula is as follows: 1% of tetramycin, 30% of pyraclostrobin, 2% of octyl phenol polyoxyethylene ether, 3% of alkylphenol polyoxyethylene ether, BX 3% of nekal, 2% of sodium dodecyl sulfate and the balance of white carbon black;

the preparation method comprises the following steps: the same as in example 1.

Example 3: 33% tetramycin zolamide mycolate water dispersible granule (1:10)

The formula is as follows: 3% of tetramycin, 30% of pyraclostrobin, 2% of alkyl naphthalene sulfonate formaldehyde, 3% of sodium lignosulfonate, 3% of sodium dodecyl sulfate, 2% of sodium lauryl sulfate, 1% of hydroxyethyl cellulose, 2% of magnesium aluminum silicate, 2% of sodium carbonate, 2% of bentonite and the balance of kaolin;

the preparation method comprises the following steps: the tetramycin, the pyraclostrobin, the dispersing agent, the wetting agent, the disintegrating agent and the filler are subjected to airflow crushing to obtain corresponding particle sizes, a semi-finished product is formed, and the semi-finished product is fed into a fluidized bed granulation dryer to be dried through a granulator, so that the water dispersible granule is prepared.

Example 4: 3% tetramycin zolamide mycolate water dispersible granule (1:5)

The formula is as follows: 0.5% of tetramycin, 2.5% of pyraclostrobin, 5% of sodium lignosulphonate, 2.5% of sodium dodecyl sulfate, 5% of urea, 5% of white carbon black and the balance of kaolin;

the preparation method comprises the following steps: the same as in example 3.

Example 5: 3% tetramycin zolamide suspending agent (1:5)

The formula is as follows: 0.5% of tetramycin, 2.5% of pyraclostrobin, 4% of polyoxyethylene polyoxypropylene ether, 1.5% of sodium lignosulfonate, 0.25% of xanthan gum, 1.5% of magnesium aluminum silicate, 1% of sodium benzoate, 3% of propylene glycol, 0.5% of silicone oil and the balance of deionized water;

the preparation method comprises the following steps: according to the formula, water, a dispersing agent and a wetting agent are uniformly mixed, a defoaming agent and an anti-freezing agent are sequentially added under the stirring state, the materials are uniformly sheared and mixed at a high speed, tetramycin and pyraclostrobin are added, the materials are continuously sheared and uniformly mixed, the materials are ground in a horizontal sand mill, the particle size of the materials is enabled to be below 5 mu m, a thickening agent is added, and the materials are sheared uniformly to obtain the suspending agent.

Example 6: 5.5% Tetramycin-Zostera Marina suspension (1:10)

The formula is as follows: 0.5% of tetramycin, 5% of pyraclostrobin, 3% of sodium polycarboxylate, 4% of sodium lignosulfonate, 3% of sodium lauryl sulfate, 2% of sodium dodecyl sulfate, 4% of diethyl ether diglycol, 2% of glycerol, 2% of organic silicon, 1% of silicone oil, 1% of gelatin, 3% of xanthan gum and the balance of deionized water;

the preparation method comprises the following steps: the same as in example 5.

Example 7: 3.5% tetramycin zolamide antibacterial ester emulsion in water (1:6)

The formula is as follows: 0.5% of tetramycin, 3% of pyraclostrobin, 2% of methyl oleate, 3% of phenyl phenol polyoxyethylene ether, 3% of triethanolamine, 3% of propylene glycol, 3% of glycerol, 2% of sodium acrylate, 1% of sodium alginate, 3% of organic silicon and the balance of deionized water;

the preparation method comprises the following steps: the preparation method comprises the steps of adding the tetramycin, the pyraclostrobin, the solvent, the emulsifier and the like into a whole, and uniformly mixing the mixture under high-speed stirring to obtain the aqueous emulsion.

Example 8: 10% tetramycin zolamide antibacterial ester emulsion in water (1:9)

The formula is as follows: 1% of tetramycin, 9% of pyraclostrobin, 3% of corn oil, 2% of phenyl phenol polyoxyethylene ether, 3% of triethanolamine, 3% of glycerol, 2% of sodium acrylate, 3% of diethyl ether diglycol, 2% of polyvinyl alcohol and the balance of deionized water;

the preparation method comprises the following steps: the same as in example 7.

Indoor activity

Example 9: combined action of tetramycin and pyraclostrobin mixing on wheat powdery mildew

The test basis is as follows: reference is made to the agricultural industry standards of the people's republic of China (NY/T1156.4-2006 and NY/T1156.6-2006): the tests were carried out in part 4 (test pot method for controlling powdery mildew in wheat) and part 6 (combined action test of compounding) of the criteria fungicide for indoor bioassay of agricultural chemicals.

Reagent to be tested: 15% tetramycin raw drug and 95% zolamide ester raw drug are provided by the research and development center of the group.

Test targets: erysiphe graminis (Erysiphe graminis).

Preparing a test material: and (3) selecting a susceptible wheat variety (rugen 502) for potting, and keeping the seedling growing to 2-3 leaf stages for later use.

Preparation of the medicament: the two raw materials are respectively dissolved by acetone, diluted by 0.1 percent of Tween 80 aqueous solution and set into 5 series of mass concentrations.

Medicament treatment: the agent is uniformly sprayed on the wheat seedlings for later use by a spraying method and is naturally dried. The test was run with a treatment containing no agent as a blank.

Inoculation and culture: inoculating for 24 hours after the agent treatment, and uniformly shaking off and inoculating powdery mildew fresh spores generated in 24 hours on the diseased wheat leaves on the treated 2-3-leaf period potted wheat seedlings. Each treatment had 3 pots, 10 plants per pot. And then culturing under the conditions that the temperature is 20-24 ℃, the humidity is 65-75%, and the illumination ratio L: D is 12:12 h.

Data investigation: and (5) carrying out graded investigation according to the blank control incidence condition.

The following classification method was used:

level 0: no disease spots;

level 1: the area of the lesion spots accounts for less than 5% of the area of the whole leaf;

and 3, level: the area of the lesion spots accounts for 6 to 15 percent of the area of the whole leaf;

and 5, stage: the area of the lesion spots accounts for 16 to 25 percent of the area of the whole leaf;

and 7, stage: the area of the lesion spots accounts for 26 to 50 percent of the area of the whole leaf;

and 9, stage: the area of the lesion spots accounts for more than 50 percent of the area of the whole leaf.

Data statistics and analysis:

and calculating disease indexes and prevention and treatment effects of the treatments according to the survey data.

The disease index is calculated according to the formula (1):

X＝[∑(Ni×i)/(N×9)]×100....................................(1)

in the formula:

x-disease index;

N_i-number of diseased leaves at each level;

i-relative grade value;

n-survey total leaf number.

The prevention and treatment effect is calculated according to the formula (2):

P＝[(CK-PT)/CK]×100........................................ (2)

in the formula:

p-control effect, unit is percentage%;

CK-placebo disease index;

PT-agent treatment disease index.

When the combined toxicity of the medicaments is measured, the synergistic effect of the mixed medicaments is evaluated according to a Sun Yunpei co-toxicity coefficient method (CTC), namely that the CTC is not more than 80 and is antagonistic, the CTC is more than 80 and is less than 120 and is additive, the CTC is not less than 120 and is synergistic, and the co-toxicity coefficient (CTC) is calculated according to a formula (3), a formula (4) and a formula (5).

ATI＝(S/M)×100.............................................(3)

In the formula:

ATI-actually measured virulence index of the combination;

S-EC of Standard drug₅₀In milligrams per liter (mg/L);

EC of M-mixtures₅₀In milligrams per liter (mg/L).

TTI＝TI_A×P_A+TI_B×P_B..........................................(4)

In the formula:

TTI-theoretical virulence index of the mixture;

TI_A-virulence index of agent a;

P_Athe percentage content of the medicament A in the mixture is percent (%);

TI_B-virulence index of the agent B;

P_Bthe percentage of the medicament B in the mixture is percentage (%).

CTC＝(ATI/TTI)×100.........................................(5)

In the formula:

CTC-co-toxicity coefficient;

ATI-actually measured toxicity index of mixed agent;

TTI-mixture theory virulence index.

Calculating test results by using DPS data processing software, and respectively calculating toxicity regression equation and EC of single test medicament and mixed medicaments with different proportions₅₀And EC₉₀And 95% confidence limit, and calculating co-toxicity coefficient (CTC) of the two medicaments in different ratios, and screening out the optimal ratio of the test medicament. The raw data for all replicates of each treatment are recorded.

The test results are shown in table 1:

TABLE 1 indoor Activity test for Tetramycin and Zostera Marina in combination on wheat powdery mildew

As shown in the indoor activity test (see table 1), the blending of tetramycin and pyraclostrobin on wheat powdery mildew EC₅₀5.0495mg/L and 0.6240mg/L respectively, and the wheat powdery mildew is sensitive to the pyraclostrobin; the co-toxicity coefficients of tetramycin and pyraclostrobin after being mixed according to the proportion of 1:30, 1:10, 1:5, 1:3, 1:1, 3:1, 5:1, 10:1 and 30:1 are all larger than 120, and the tetramycin and pyraclostrobin have a synergistic effect on preventing and treating wheat powdery mildew.

Pesticide effect in field

Example 10: tetramycin and pyraclostrobin mixed field pesticide effect for preventing and treating wheat powdery mildew

Test varieties: a robust 502.

The control object is: wheat powdery mildew.

Test site: the planting land in the Tubai county of south-Yang city, Henan province has the pesticide application time of 4 Yueyu in 2020.

The test method comprises the following steps: the cell area is 25m by random block arrangement²4 repeats. Five-point sampling is fixed on diagonal line of each cell, and each point looks for 0.25m²Plants, post-heading surveyFlag leaf of the strain and the first leaf under the flag leaf. Applying the powder for the initial stage of wheat powdery mildew by a conventional spraying method, applying the powder for one time after 9d after application, applying the powder for 2 times in total, determining strains before application to investigate the occurrence condition of the wheat powdery mildew, recording disease indexes, investigating once after 9d after the first application and 10d after the second application, and calculating each prevention effect.

And (4) carrying out grading investigation, wherein the grading standard refers to the field efficacy test criterion GB/T17980.22-2000, and the test data are analyzed by adopting a Duncan's new re-polar difference method.

Adopting a 9-level classification method:

grading method of powdery mildew (taking leaves as unit):

level 0: no disease spots;

and 3, level: the lesion area accounts for 6-15% of the whole leaf area;

and 5, stage: the area of the lesion spots accounts for 16-25% of the area of the whole leaf;

and 7, stage: the area of the lesion spots accounts for 26-50% of the area of the whole leaf;

The drug effect is calculated according to the formula (1) and the formula (2):

(1) disease index ∑[ (number of diseased leaves at each stage × number of relative stage)/(number of investigated total leaves × 9) ] × 100

(2) The prevention and treatment effect (%) is [1- (disease index before drug administration in blank control area x disease index after drug administration in treatment area)/(disease index after drug administration in blank control area x disease index before drug administration in treatment area) ] × 100

Safety investigation:

during the test period, the crops are observed whether the crops have phytotoxicity symptoms, if phytotoxicity records the phytotoxicity degree, the crop phytotoxicity symptoms (dwarfing, chlorosis, malformation and the like) are accurately described, and in addition, the beneficial effects on the crops are recorded.

TABLE 2 field drug effect of tetramycin and pyraclostrobin mixed for preventing and treating wheat powdery mildew

The field efficacy showed (see table 2): under the same environmental conditions, the field effect of each treatment is 60.64-89.15 percent after 9 days of the first application of different treatments for preventing and treating wheat powdery mildew, and 10g/hm of 5.5 percent of tetramycin-zolamide mycolate suspending agent is sprayed²、25g/hm²、40g/hm²The control effects of the three different dosages on wheat powdery mildew are different, namely 77.71%, 83.63% and 89.15%, which are respectively superior or obviously superior to single dosage and local conventional medicament (60% azoxystrobin water dispersible granule), wherein the dosage of the 5.5% tetramycin-zolamide antibacterial suspending agent is 40g/hm²The control effect is best. The whole field effect of each treatment is 62.11-90.45% after 10 days of the second pesticide application, and 5.5% of tetramycin-zolamide mycolate suspending agent is sprayed, 10g/hm²、25g/hm²、40g/hm²The control of wheat powdery mildew after treatment of three different doses is 78.06%, 84.64% and 90.45% respectively.

The results of the indoor test and the field test show that the tetramycin and the pyraclostrobin are compounded in a ratio of 1:10 to achieve a good effect of preventing and treating the wheat powdery mildew, the effect of preventing and treating the wheat powdery mildew by using a single agent of the tetramycin and the pyraclostrobin is obviously lower than that of a compound agent, and in the test process, the agent is safe to wheat under the test concentration and has no phytotoxicity. Therefore, the tetramycin and the pyraclostrobin are compounded in a ratio of 1:10, so that the tendency that the resistance of powdery mildew of wheat rises year by year can be relieved, the control effect of the powdery mildew of wheat is improved, and the service life of the medicament is prolonged.

Claims

1. The bactericidal composition is characterized by comprising an effective component A and an effective component B, wherein the effective component A is tetramycin, and the effective component B is pyraclostrobin.

2. The bactericidal composition according to claim 1, wherein the mass ratio of the tetramycin serving as an active ingredient to the pyraclostrobin serving as an active ingredient is 1: 50-50: 1.

3. The bactericidal composition according to claim 1, wherein the mass ratio of the tetramycin serving as an active ingredient to the pyraclostrobin serving as an active ingredient is 1: 30-30: 1.

4. The bactericidal composition according to claim 1, wherein the mass ratio of the tetramycin serving as an active ingredient to the pyraclostrobin serving as an active ingredient is 1: 30-10: 1; preferably, the mass ratio of the active ingredients tetramycin to the pyraclostrobin is 1: 10-3: 1.

5. The bactericidal composition according to claim 1, wherein the sum of the mass of the effective components tetramycin and pyraclostrobin accounts for 1-80% of the composition; preferably 1% to 50%.

6. The bactericidal composition according to any one of claims 1 to 5, wherein the bactericidal composition further comprises an auxiliary pesticide component in addition to the active ingredient.

7. The bactericidal composition of claim 6, wherein the bactericidal composition is formulated into an agriculturally acceptable formulation, including any one of powders, dispersible tablets, granules, soluble powders, soluble granules, soluble tablets, emulsifiable powders, emulsifiable granules, water dispersible granules, wettable powders, microencapsulated granules, powders, macrogranules, water dispersible tablets, microcapsule suspensions, dispersible solutions, emulsifiable concentrates, oil emulsions, aqueous emulsions, microemulsions, dispersible oil suspensions, oil dispersible powders, suspensions, suspoemulsions, soluble solutions, ultra-low volume solutions, seed coating suspensions, dry seed treatment powders, dispersible seed treatment powders, seed treatment suspensions, seed treatment liquids; the preferable preparation formulation is wettable powder, water dispersible granules, suspending agent and aqueous emulsion.

8. The use of the bactericidal composition and the preparation thereof according to any one of claims 1 to 7 for controlling diseases on crops.

9. The disease on crop plants of claim 8 is wheat powdery mildew.