CN112457124A - Method for preventing and controlling banana wilt by using green manure and bio-organic fertilizer - Google Patents
Method for preventing and controlling banana wilt by using green manure and bio-organic fertilizer Download PDFInfo
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- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
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- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
- C05F17/20—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation using specific microorganisms or substances, e.g. enzymes, for activating or stimulating the treatment
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Abstract
The invention relates to the technical field of agricultural pest control, and particularly discloses a method for preventing and controlling banana wilt by using green manure and a bio-organic fertilizer, wherein the green manure is sowed in vacant lands among banana rows while banana planting is carried out according to a conventional method; applying a bio-organic fertilizer in the middle period of banana cultivation, vegetative growth and early bud extraction, wherein the fermentation raw materials of the bio-organic fertilizer comprise the following components in parts by weight: 10-20 parts of livestock and poultry manure, 20-30 parts of crop straw, 10-20 parts of molasses fermentation concentrated solution, 10-20 parts of shell powder and 1-5 parts of composite zymophyte; the composite zymophyte comprises the following components in parts by weight: 3-5 parts of bacillus subtilis, 2-4 parts of bacillus polymyxa, 3-6 parts of mortierella elongata and 1-2 parts of trichoderma. The method interplanting the green manure in the banana field and improving the formula of the biological fertilizer can adjust the soil environment and improve the disease-resistant effect of banana wilt while providing the fertility required by banana growth.
Description
Technical Field
The invention belongs to the technical field of crop pest control, and particularly relates to a method for preventing and controlling banana wilt by using green manure and a bio-organic fertilizer.
Background
Banana vascular wilt, also known as panama disease and yellow leaf disease, is known as banana cancer, is a typical fungal soil-borne disease causing plant death by damaging vascular bundles and causing plant death due to fusarium oxysporum special infection, and has high prevention and control difficulty. At present, the methods mainly adopted for preventing and controlling the banana wilt comprise chemical agents, crop rotation, breeding disease-resistant varieties, biological prevention and control, appropriate agricultural measures and the like. Wherein, the biological control is a relatively safe, environment-friendly and efficient control measure. In recent years, biological organic fertilizers are popularized and applied, but the prevention and control effect is not obvious enough when the organic fertilizers are used for biological prevention and control of banana wilt.
Disclosure of Invention
The invention aims to provide a method for preventing and controlling banana vascular wilt by using green manure and a biological organic fertilizer, thereby overcoming the defect that the existing organic fertilizer has poor effect on preventing and controlling banana vascular wilt.
In order to realize the purpose, the invention provides a method for preventing and controlling banana wilt by utilizing green manure and bio-organic fertilizer, banana is planted according to a conventional method, and simultaneously,
sowing green manure in idle lands among the banana rows;
applying a bio-organic fertilizer in the middle period of banana cultivation, vegetative growth and early bud extraction, wherein the fermentation raw materials of the bio-organic fertilizer comprise the following components in parts by weight: 10-20 parts of livestock and poultry manure, 20-30 parts of crop straw, 10-20 parts of molasses fermentation concentrated solution, 10-20 parts of shell powder and 1-5 parts of composite zymophyte; the composite zymophyte comprises the following components in parts by weight: 3-5 parts of bacillus subtilis, 2-4 parts of bacillus polymyxa, 3-6 parts of mortierella elongata and 1-2 parts of trichoderma. The other methods which are not stated, such as the variety, planting density, management method and the like of the banana adopt the prior art.
Preferably, in the above technical scheme, the green manure is at least one of soybean, mung bean, black bean, Styrax, marigold, coreopsis, paspalum broadleaf, sunflower, Selaginella sanguinea, sesbanum, Dolabella labrata, safflower, ryegrass, oat grass, cogongrass, vetch, white clover, red clover and rape. The interplanting can be carried out by adopting a single variety of green manure, and the interplanting can also be carried out by adopting two or more green manure varieties simultaneously.
Preferably, houttuynia cordata is planted among the banana rows in an idle manner, the houttuynia cordata is planted by cutting, and the row spacing of the planted plants is (10-12) × (8-10) cm. The houttuynia cordata has fishy smell, can effectively repel pests such as banana weevil, banana bract and aphid, and can reduce the application of chemical pesticides. The odor emitted by herba Houttuyniae mainly comprises houttuynine sodium bisulfite, lauraldehyde, etc., has antibacterial and insecticidal effects, has inhibiting effect on soil-borne diseases such as banana wilt pathogen, and can improve disease resistance of banana wilt disease together with residue of green manure.
Preferably, the houttuynia cordata is planted in 4-5 months, and green manure is sowed around the houttuynia cordata after the houttuynia cordata grows out.
Preferably, 1000-1500kg of the bio-organic fertilizer is applied per mu.
Preferably, the bacillus subtilis, the bacillus polymyxa and the mortierella elongata are obtained by separating, purifying and screening banana rhizosphere soil. Compared with the conventional strains, the bacillus subtilis, the bacillus polymyxa and the mortierella elongata separated from the banana rhizosphere soil have better disease resistance.
Preferably, the molasses alcohol concentrated solution and the compound zymophyte are diluted with water and then sprayed on the livestock and poultry manure and the crop straws for fermentation, composting is carried out according to the rule that one layer of crop straws is one layer of livestock and poultry manure, and the water content of the crop straws is controlled to be 50-70%; and fermenting the fermentation raw materials at the temperature of between 30 and 65 ℃ for 20 to 30 days to obtain the biological organic fertilizer.
Preferably, the livestock and poultry manure is pig manure or chicken manure, and the crop straw is banana straw, corn straw or sugarcane straw.
Preferably, the calcium magnesium phosphate fertilizer is applied during the banana cultivation, and 0.5-1kg of calcium magnesium phosphate fertilizer is applied to each banana. The calcium magnesium phosphate fertilizer can not only increase the fertility of soil, but also improve the disease resistance of banana vascular wilt.
Preferably, the bananas are crop-rotated with sugarcane. The crop rotation with the sugarcane can reduce the incidence rate of the next banana wilt and improve the control effect of the wilt.
Compared with the prior art, the invention has the following beneficial effects:
1. according to the invention, the green manure and the houttuynia cordata are interplanted among the banana rows, so that insect pests can be driven, the secretion of the houttuynia cordata can be utilized to enhance the sterilization effect of the bananas on soil-borne diseases, and the disease resistance effect of the banana wilt can be improved together with the residue rotten matter of the green manure. Meanwhile, the planting of the green manure can increase the organic matters of the soil, improve the soil fertility and promote the growth of bananas.
2. The biological organic fertilizer adopts bacillus subtilis, bacillus polymyxa and mortierella elongata which are separated and extracted from banana rhizosphere soil as fermentation strains, and is compounded with trichoderma as a fermentation microbial inoculum, so that the effect of the organic fertilizer is better than that of an organic fertilizer prepared by a conventional fermentation microbial inoculum. The fermentation microbial inoculum can be rapidly propagated under the promotion of molasses fermentation concentrated solution, and is fully contacted with livestock and poultry manure, crop straws and the like, so that the fermentation is more sufficient, the fermentation microbial inoculum is beneficial to contacting and decomposing cellulose, crude protein rich in mulberry twig skins and organic substances such as lignin and the like contained in straws, a large amount of micromolecule nutrient substances and nitrogen-containing element ions easy to absorb by plants are generated, the soil is improved, water and fertilizer are preserved, and the hardening and deterioration of soil quality are relieved; the shell powder is rich in inorganic salts such as calcium, magnesium and the like, can further improve the nutrition of the organic fertilizer, and improves the resistance of the bananas.
Detailed Description
The following detailed description of specific embodiments of the invention is provided, but it should be understood that the scope of the invention is not limited to the specific embodiments.
Example 1 screening and assay of microbial Agents
Preparation of bacterial liquid
Taking rhizosphere soil of a healthy banana garden as a research object, separating, purifying, screening to obtain Bacillus subtilis, Paenibacillus polymyxa and Mortierella elongata SX, activating, respectively selecting 1-ring activated bacterial colonies, inoculating into 1000ml of LB culture solution, and culturing in a constant-temperature oscillation incubator at 28 ℃ for 24h at 160rpm to form a bacterial solution.
Several 100mL conical flasks were prepared, and 20mL of PDA (pH adjusted to 7) culture medium was poured into the 100mL conical flasks, and the flasks were sterilized at high temperature after being filled.
Inoculating the cultured three functional bacteria into a 100mL conical flask filled with 20mL of PDA culture solution according to the proportion of 1:100, and culturing for 48h at 30 ℃ and the shaking table speed of 120rpm to obtain a seed solution.
(II) test of prevention and treatment effects of microbial inoculum on banana plant growth and banana wilt
1.1 materials and methods
1.1.1 test materials
Banana test: the tested banana variety is the bacon banana and 6 leaves.
Test microbial inoculum: FOC4 pathogenic bacterium, spore concentration 106cfu/ml; antagonistic bacteria Bacillus subtilis KC with the thallus concentration of 107cfu/ml; antagonistic bacterium Paenibacillus polymyxa DN with the thallus concentration of 107cfu/ml; mortierella elongata CB at a cell concentration of 107cfu/ml; the composite bacteria FH1 (Bacillus subtilis, Paenibacillus polymyxa) and Mortierella elongata SX (Mortierella elongata SX) are mixed according to the proportion that the number of colony forming units is 1:0.8: 1), and the thallus concentration is 107cfu/ml；
Complex bacteria FH2 (Bacillus subtilis), Paenibacillus polymyxa (Paenibacillus polymyxa) and Mortierella elongata (Mortierella elongata SX) with the number of colony forming units of 1:
1.2: 1.4) in a ratio of 10. ang. in a ratio of the total amount of the components7cfu/ml；
Complex bacteria CD (Bacillus subtilis) and Paenibacii polymyxa (Paenibaci)llus polymyxa) at a bacterial concentration of 10. mu.l/l in a ratio of 1:1 in terms of the number of colony forming units7cfu/ml)。
Test soil: the soil is collected from a healthy banana garden soil of a test base of the Taiping town Xinliancun in the Wuming district, and each bucket is 7.5 kg.
Test time: transplanting at 25 months 8 in 2016, and spraying 3 times every 20 days a ternary compound fertilizer (N: P: K: 15: 15: 15) with concentration of 2.5%. Carrying out root injury treatment on 1 day at 11 months, simultaneously carrying out FOC4 strain inoculation on leaves, and respectively inoculating 100ml of experimental microbial inoculum with the concentration of 10 strains to the treatment 2-7 after 10 days7cfu/ml。
1.1.2 test design
The test set 6 treatments, each of which planted 30 pots, were randomly arranged. The treatment is respectively as follows:
treatment 1: control, 100ml of pathogenic bacteria inoculated with FOC4 (spore concentration 10)6cfu/ml)
And (3) treatment 2: 100ml of pathogenic bacteria FOC4 + 100ml of bacillus subtilis KC
And (3) treatment: 100ml of pathogenic bacteria FOC4 + DN 100ml of paenibacillus polymyxa
And (4) treatment: 100ml of pathogenic bacteria FOC4 + 100ml of Mortierella elongata CB
And (4) treatment 5: 100ml pathogenic bacteria FOC4 inoculation + composite bacteria FH 1100 ml
And (6) treatment: 100ml pathogenic bacteria FOC4 inoculation + composite bacteria FH 2100 ml
And (7) treatment: 100ml of FOC4 pathogenic bacteria and 100ml of composite bacteria CD
Test site: glass greenhouse of the test base of Guangxi academy of agricultural sciences.
1.2 prevention and treatment Effect of the bacteria on the banana wilt disease of the banana plants
As shown in Table 1, different treatments had different effects on disease index and relative prevention and control effects of banana vascular wilt. The disease indexes of 7 treatments are represented by that composite bacteria FH1 is more than composite bacteria FH2 is more than composite bacteria CD is more than Bacillus subtilis KC is more than Paenibacillus polymyxa DN is more than Mortierella elongata CB is less than a control. The disease index is 0.6 by applying the bacillus subtilis, and the prevention and treatment effect of the banana vascular wilt reaches 27.97 percent; the disease index of the paenibacillus polymyxa is 0.733, and the control effect of the banana vascular wilt is 19.9%; the disease index of the Mortierella elongata is 1.167, and the prevention and treatment effect of the banana vascular wilt is 12 percent; the disease index of the compound bacteria FH1 is 0.333, and the prevention and treatment effect of the banana vascular wilt is 60%; the disease index of the compound bacteria FH2 is 0.367, the control effect of the banana vascular wilt is 55.9 percent obvious, and the effect of reducing the banana vascular wilt by applying the compound bacteria consisting of the bacillus subtilis, the paenibacillus polymyxa and the mortierella elongata is obviously better than that of independently using the bacillus subtilis, the paenibacillus polymyxa, the mortierella elongata or the compound bacteria consisting of only the bacillus subtilis and the paenibacillus polymyxa.
TABLE 1 situation of the strains on the prevention and control of banana vascular wilt
| Treatment of | Index of disease condition | Relative prevention effect% |
| KC | 0.6 | 27.97 |
| DN | 0.667 | 19.90 |
| CB | 0.733 | 12.00 |
| FH1 | 0.333 | 60.00 |
| FH2 | 0.367 | 55.90 |
| CD | 0.5 | 39.97 |
| Control | 0.833 |
Example 2 method for preventing and controlling banana wilt by using green manure and bio-organic fertilizer
(one) test site: banana planting base of Yufa agriculture science and technology development limited company in Xinjiucan, Taiping town of Wuming city. And (3) deeply loosening and ridging the planting field and digging a drainage ditch according to a conventional method, wherein the planting time of the bananas is 3, 9 and 2018, and the planting cup seedlings are Brazil bananas and 7-8 leaf seedlings. Planting according to a conventional method, wherein the planting density is 135 plants/mu. The planting field is divided into 4 areas, each area is 2 mu, the planting is carried out according to the following method, and the rest parts which are not stated are managed according to the conventional method.
Test zone 1:
(1) and applying 1000kg of bio-organic fertilizer per mu in the middle period of banana cultivation, vegetative growth and early bud drawing. The fermentation raw materials of the biological organic fertilizer comprise the following components in parts by weight: 20 parts of livestock and poultry manure, 20 parts of crop straws, 10 parts of molasses fermentation concentrated solution, 20 parts of shell powder and 1 part of composite zymophyte; the composite zymophyte comprises the following components in parts by weight: 3 parts of bacillus subtilis, 4 parts of bacillus polymyxa, 6 parts of mortierella elongata and 1 part of trichoderma.
The bacillus subtilis, the bacillus polymyxa and the mortierella elongata are obtained by separating, purifying and screening banana rhizosphere soil. The screening method is described in reference to example 1
The preparation of the organic fertilizer is carried out according to the following method: diluting the molasses alcohol concentrated solution and the compound zymophyte with water, spraying the diluted molasses alcohol concentrated solution and the compound zymophyte on the livestock manure and crop straws for fermentation, and composting according to a rule that one layer of crop straws is one layer of livestock manure, wherein the water content of the crop straws is controlled to be 50-70%; and fermenting the fermentation raw materials at 30-65 ℃ for 25 days to obtain the biological organic fertilizer. The livestock and poultry manure is pig manure or chicken manure, and the crop straw is banana straw, corn straw or sugarcane straw.
(2) And a calcium magnesium phosphate fertilizer is applied during banana cultivation, wherein the calcium magnesium phosphate fertilizer is purchased from the market, and 0.5-1kg of calcium magnesium phosphate fertilizer is applied to each banana.
(3) And in 2018, in 4 and 16 months, planting houttuynia cordata in vacant spaces among banana rows, wherein the houttuynia cordata is planted by cutting, and the plant row spacing of the planting is 10 x 10 cm. After the houttuynia cordata grows out, green manure is sowed around the houttuynia cordata. The green manure is marigold. Of course, other varieties of green manure can also be sown.
The rest is managed by a conventional method.
Test zone 2:
(1) and applying 1500kg of bio-organic fertilizer per mu in the middle period of banana cultivation, vegetative growth and early bud drawing. The fermentation raw materials of the biological organic fertilizer comprise the following components in parts by weight: 10 parts of livestock and poultry manure, 30 parts of crop straws, 20 parts of molasses fermentation concentrated solution, 20 parts of shell powder and 5 parts of composite zymophyte; the composite zymophyte comprises the following components in parts by weight: 5 parts of bacillus subtilis, 3 parts of bacillus polymyxa, 3 parts of mortierella elongata and 2 parts of trichoderma.
The bacillus subtilis, the bacillus polymyxa and the mortierella elongata are obtained by separating, purifying and screening banana rhizosphere soil. The screening method is described in example 1.
The preparation of the organic fertilizer is carried out according to the following method: diluting the molasses alcohol concentrated solution and the compound zymophyte with water, spraying the diluted molasses alcohol concentrated solution and the compound zymophyte on the livestock manure and crop straws for fermentation, and composting according to a rule that one layer of crop straws is one layer of livestock manure, wherein the water content of the crop straws is controlled to be 50-70%; and fermenting the fermentation raw materials at the temperature of 30-65 ℃ for 30 days to obtain the biological organic fertilizer. The livestock and poultry manure is pig manure or chicken manure, and the crop straw is banana straw, corn straw or sugarcane straw.
(2) And a calcium magnesium phosphate fertilizer is applied during banana cultivation, wherein the calcium magnesium phosphate fertilizer is purchased from the market, and 0.5-1kg of calcium magnesium phosphate fertilizer is applied to each banana.
(3) And in 2018, in 4 and 16 months, planting houttuynia cordata in vacant spaces among banana rows, wherein the houttuynia cordata is planted by cutting, and the plant row spacing of the planting is 10 x 10 cm. After the houttuynia cordata grows out, green manure is sowed around the houttuynia cordata. The green manure is marigold. Of course, other varieties of green manure can also be sown.
The rest is managed by a conventional method.
Test zone 3:
(1) and applying 1500kg of bio-organic fertilizer per mu in the middle period of banana cultivation, vegetative growth and early bud drawing. The fermentation raw materials of the biological organic fertilizer comprise the following components in parts by weight: 30 parts of crop straws and 4 parts of bacillus subtilis. Fermenting by conventional composting method.
(2) And 4, in 2018, in 4, 16, planting green manure in idle lands among banana rows, wherein the green manure is marigold. Of course, other varieties of green manure can also be sown.
The rest is managed by a conventional method. In other words, green manure is interplanted between banana rows in the test area 3, but an organic fertilizer only containing bacillus subtilis is used in the banana planting period.
Test zone 4:
bananas are planted in the test area according to a conventional method, and green manure and houttuynia cordata are not interplanted. Organic fertilizer purchased from the market is applied in the middle period of banana cultivation, vegetative growth and bud extraction. And nitrogen and phosphorus fertilizers purchased from the market are applied in the planting period.
(II) investigation of prevention and control of banana vascular wilt
The incidence of banana vascular wilt in the four test areas is respectively investigated, and as can be seen from table 2, the incidence of banana vascular wilt treated by the method is less than 3%, 7.4% lower than that of the banana vascular wilt treated by merely interplanting green manure and applying a conventional bio-organic fertilizer, and 14% lower than that of the banana vascular wilt treated by not interplanting green manure and applying a conventional bio-organic fertilizer. In addition, the application of the chemical pesticide interplanting the houttuynia cordata is reduced by 2-3 times compared with the chemical pesticide interplanting the houttuynia cordata, and the usage amount of the chemical pesticide is reduced by 15.1-20.2 percent compared with the chemical pesticide interplanting the houttuynia cordata.
TABLE 2 occurrence of banana vascular wilt
(II) Banana yield
As can be seen from the table 3, the yield of bananas treated by the method per mu is 3294 kg, and the effect is obviously improved compared with 688.5 kg of bananas interplanted with green manure only and applied with a conventional bio-organic fertilizer, and compared with 850.5 kg of bananas interplanted with green manure and houttuynia cordata.
TABLE 3 influence of bio-organic fertilizer on banana yield in old planting area
(III) survey of soil physical and chemical Properties
The soil of test area 1 and test area 4 was selected for investigation. As can be seen from table 4, the contents of soil quick-acting nitrogen, quick-acting phosphorus, quick-acting potassium, exchangeable calcium, exchangeable magnesium, organic matter, soil total nitrogen and total phosphorus are significantly increased in test area 1 compared with test area 4.
TABLE 4 Effect of soil physicochemical Properties of applied Bio-organic Fertilizer
| Treatment of | LF1 | LCK |
| All N (%) | 0.274 | 0.156 |
| All P (%) | 0.892 | 0.361 |
| Whole K (%) | 0.174 | 0.148 |
| Speed N (mg/kg) | 71 | 62 |
| Speed P (mg/kg) | 131 | 68 |
| Speed K (mg/kg) | 503 | 302 |
| pH | 7.35 | 7.06 |
| Organic matter (g/kg) | 65.7 | 37.5 |
| Exchangeable Ca (cmol/kg) | 12.81 | 11.88 |
| Exchangeable mg (cmol/kg) | 0.31 | 0.13 |
In conclusion, the method interplanting the green manure and the houttuynia cordata in the banana land and improving the formula of the biological fertilizer can provide the fertility required by the growth of the bananas, improve the yield of the bananas, adjust the soil environment and improve the disease-resistant effect of the banana wilt.
The foregoing descriptions of specific exemplary embodiments of the present invention have been presented for purposes of illustration and description. It is not intended to limit the invention to the precise form disclosed, and obviously many modifications and variations are possible in light of the above teaching. The exemplary embodiments were chosen and described in order to explain certain principles of the invention and its practical application to enable one skilled in the art to make and use various exemplary embodiments of the invention and various alternatives and modifications as are suited to the particular use contemplated. It is intended that the scope of the invention be defined by the claims and their equivalents.
Claims (10)
1. A method for preventing and controlling banana wilt by using green manure and bio-organic fertilizer, which is characterized in that,
sowing green manure in idle lands among the banana rows;
applying a bio-organic fertilizer in the middle period of banana cultivation, vegetative growth and early bud extraction, wherein the fermentation raw materials of the bio-organic fertilizer comprise the following components in parts by weight: 10-20 parts of livestock and poultry manure, 20-30 parts of crop straw, 10-20 parts of molasses fermentation concentrated solution, 10-20 parts of shell powder and 1-5 parts of composite zymophyte; the composite zymophyte comprises the following components in parts by weight: 3-5 parts of bacillus subtilis, 2-4 parts of bacillus polymyxa, 3-6 parts of mortierella elongata and 1-2 parts of trichoderma.
2. The method for preventing and controlling banana vascular wilt using green manure and bio-organic fertilizer as claimed in claim 1, wherein the green manure is at least one of soybean, mung bean, black bean, Styrax, Tagetes erecta, Sphaerotheca latifolia, Helichrysum italicum, Sedum brasiliensis, Sedum palustris, Coccodonia lacustris, Carthamus tinctorius, ryegrass, oat grass, Cymbopogon citratus, vetch, white clover, red clover and rape.
3. The method for preventing and controlling banana vascular wilt using green manure and bio-organic fertilizer as claimed in claim 1, wherein houttuynia cordata is further planted in vacant spaces among banana rows, wherein houttuynia cordata is planted by cutting, and the row spacing of the planted plants is (10-12) × (8-10) cm.
4. The method for preventing and controlling banana vascular wilt using green manure and bio-organic fertilizer as claimed in claim 3, wherein said houttuynia cordata is planted in 4-5 months and green manure is sown around the houttuynia cordata after it has grown.
5. The method for preventing and controlling banana vascular wilt using green manure and bio-organic fertilizer as claimed in claim 1, wherein 1000-1500kg of bio-organic fertilizer is applied per mu.
6. The method for preventing and controlling banana vascular wilt by using green manure and bio-organic fertilizer as claimed in claim 1, wherein the bacillus subtilis, bacillus polymyxa and mortierella elongata are obtained by separating, purifying and screening banana rhizosphere soil.
7. The method for preventing and controlling banana vascular wilt by using green manure and bio-organic fertilizer as claimed in claim 1, wherein said molasses alcohol concentrated solution and said composite fermentation bacteria are diluted with water and then sprayed on said livestock manure and crop straw for fermentation, and composting is performed according to the rule of one layer of crop straw and one layer of livestock manure, and the water content of the crop straw is controlled to be 50-70%; and fermenting the fermentation raw materials at the temperature of between 30 and 65 ℃ for 20 to 30 days to obtain the biological organic fertilizer.
8. The method for preventing and controlling banana vascular wilt by using green manure and bio-organic fertilizer as claimed in claim 1, wherein the livestock manure is pig manure or chicken manure, and the crop straw is banana straw, corn straw or sugarcane straw.
9. The method for preventing and controlling banana vascular wilt disease by using green manure and bio-organic fertilizer as claimed in claim 1, wherein calcium magnesium phosphate fertilizer is applied during cultivation of banana, and 0.5-1kg of calcium magnesium phosphate fertilizer is applied to each banana.
10. The method for preventing and controlling banana vascular wilt disease through green manure and bio-organic fertilizer as claimed in claim 1, wherein the banana is crop-rotated with sugarcane.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN115226455A (en) * | 2022-07-22 | 2022-10-25 | 海南宝绿春农业开发有限公司 | Fertilizing method for conditioning banana continuous cropping obstacle soil |
| CN116746353A (en) * | 2023-07-10 | 2023-09-15 | 中国热带农业科学院热带作物品种资源研究所 | Method for preventing and controlling banana vascular wilt by fertilizer adjustment |
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| US20110214463A1 (en) * | 2009-09-18 | 2011-09-08 | Nanjing Agricultural University | Antagonistic bacteria for controlling the fusarium wilt of continuous cropping banana and their microbial organic fertilizer |
| CN108753664A (en) * | 2018-06-28 | 2018-11-06 | 广东省农业科学院农业资源与环境研究所 | A kind of biocontrol agent, biological organic fertilizer and its application |
| US20200131096A1 (en) * | 2018-10-29 | 2020-04-30 | Sustainable Community Development, Llc | Biofertilizer Composition and Method of Manufacture |
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| US20110214463A1 (en) * | 2009-09-18 | 2011-09-08 | Nanjing Agricultural University | Antagonistic bacteria for controlling the fusarium wilt of continuous cropping banana and their microbial organic fertilizer |
| CN108753664A (en) * | 2018-06-28 | 2018-11-06 | 广东省农业科学院农业资源与环境研究所 | A kind of biocontrol agent, biological organic fertilizer and its application |
| US20200131096A1 (en) * | 2018-10-29 | 2020-04-30 | Sustainable Community Development, Llc | Biofertilizer Composition and Method of Manufacture |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115226455A (en) * | 2022-07-22 | 2022-10-25 | 海南宝绿春农业开发有限公司 | Fertilizing method for conditioning banana continuous cropping obstacle soil |
| CN116746353A (en) * | 2023-07-10 | 2023-09-15 | 中国热带农业科学院热带作物品种资源研究所 | Method for preventing and controlling banana vascular wilt by fertilizer adjustment |
| CN116746353B (en) * | 2023-07-10 | 2024-04-19 | 中国热带农业科学院热带作物品种资源研究所 | Method for preventing and controlling banana vascular wilt by fertilizer adjustment |
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