CN112403547A

CN112403547A - Liquid transfer device suitable for continuous sample adding

Info

Publication number: CN112403547A
Application number: CN202011498341.8A
Authority: CN
Inventors: 梅丛进; 余传信; 杨莹莹; 宋丽君; 董盼盼; 周永华; 于汉寿
Original assignee: Jiangsu Institute of Parasitic Diseases
Current assignee: Jiangsu Institute of Parasitic Diseases
Priority date: 2020-12-17
Filing date: 2020-12-17
Publication date: 2021-02-26
Anticipated expiration: 2040-12-17
Also published as: CN112403547B

Abstract

The invention discloses a liquid transfer device suitable for continuous sample adding, which comprises a shell, a piston assembly, a pushing device, a sample feeding assembly and a counting assembly, wherein the piston assembly, the pushing device and the sample feeding assembly are arranged in the shell; the sample feeding assembly comprises a sample tube, a liquid outlet pump and a liquid delivery tube, the liquid delivery tube is communicated with a pump port of the liquid outlet pump, the liquid delivery tube vertically extends out of the shell, the liquid outlet pump is connected to a cover body of the sample tube, and the top of the liquid outlet pump is connected with a pushing rod; the counting assembly comprises a counter and a touch assembly, the counter is fixed on the shell, the touch assembly is connected onto the shell, the counter comprises a counting disc sequence consisting of a shell and two counting discs, a display window is arranged on the shell, a counting button is arranged at the top of the shell, and a reset knob is arranged on the right side of the shell. The liquid transfer device is mainly designed aiming at the sample adding process of a 96-hole well plate, can obviously reduce the error rate and ensure the accuracy of an operation result.

Description

Liquid transfer device suitable for continuous sample adding

Technical Field

The invention relates to the field of sample adding devices, in particular to a liquid transfer device suitable for continuous sample adding.

Background

The common liquid-transfering device usually completes one-time sample-adding after one-time sampling, the sample-adding process is discontinuous, when the whole-hole sequential sample adding operation needs to be carried out on a slot plate structure with 96 slots, namely a slot plate structure with more slots, the process needs to be repeated for many times, because the sample is added one by one, the sample adding amount is large, the repeatability is high, operators often forget the specific hole slot position of the sample adding completion on the way of sample adding in the continuous sampling and sample adding process, especially under the condition that the sample or other contents are originally existed in the hole slot, the sample adding position is difficult to be judged simply from whether the hole slot is empty or not, in the middle of sample adding, once an operator is interrupted by other things or other people to sample adding process, the operator can easily forget (or wrongly remember) the number of times of sample adding, which can affect the operation result, even the sample adding process needs to be abandoned to carry out the operation of sample adding again, which not only can cause the waste of materials, but also can excessively consume the energy of the operator.

Disclosure of Invention

The invention aims to provide a liquid transfer device which is reasonable in structural design and suitable for continuous sample adding.

The invention is realized by the following steps: a liquid-transfering device suitable for continuous sample application comprises a shell, a piston assembly, a pushing device and a sample-feeding assembly which are arranged in the shell from top to bottom in sequence, and a counting assembly arranged outside the shell; the sample feeding assembly is arranged below the pushing device and comprises a sample tube, a liquid outlet pump and a liquid delivery tube, the liquid delivery tube is communicated with a pump port of the liquid outlet pump and integrally arranged with the pump port of the liquid outlet pump, the liquid delivery tube vertically extends out of the shell, the liquid outlet pump is fixedly connected to a sample tube cover body, the sample tube cover body is connected with the top of the sample tube in an inserting manner, and the top of the liquid outlet pump is detachably and fixedly connected with a pushing rod at the bottom of the pushing device; the counting assembly comprises a counter and a touch assembly, the counter is fixed on the shell, the touch assembly is connected onto the shell, the counter comprises a counting disc sequence consisting of the shell and two counting discs, a display window is arranged on the shell, a counting button is arranged at the top of the shell and below the touch assembly, and a reset knob is arranged on the right side of the shell.

Further, the counting disc sequence is composed of a first counting disc on the outer circumference of which numerals 0 to 12 are marked at a predetermined angle, and a second counting disc arranged adjacent to the first counting disc along the same axis and letters a to H are marked at a predetermined angle on the outer circumference of the second counting disc, the counting button is pressed 12 times, the first counting disc is rotated by a complete revolution, and the second counting disc rotates a part by a predetermined angle and clockwise from the previous letter to the next letter.

Further, the piston assembly comprises a piston rod, the top of the piston rod is fixedly connected with a push button, an upper boss is arranged on the lower portion of the piston rod, a lower boss is fixedly connected in the shell below the upper boss, the bottom end of the piston rod penetrates through the lower boss and is connected with the pushing device, and a reset spring is arranged on the piston rod between the upper boss and the lower boss.

Furthermore, the top of the liquid outlet pump is fixedly connected with the pushing rod in an inserting mode.

Furthermore, the shell comprises an upper shell and a lower shell, the upper shell is connected with the lower shell in an inserting mode, a middle platform is arranged in the lower shell, a sample tube socket is arranged on the middle platform, an inserting hole adaptive to the shape of the bottom of the sample tube is formed in the middle of the sample tube socket, and the bottom of the sample tube is inserted and fixed in the inserting hole.

Further, the touch assembly comprises a sleeve and a touch rod, the sleeve is fixedly connected to the shell, the touch rod is movably sleeved in the sleeve, the diameter of the touch rod is matched with the inner diameter of the sleeve, and a return spring is arranged between the bottom of the top disc of the touch rod and the top surface of the sleeve.

Furthermore, a center hole is formed in the middle of the middle platform, a guide pipe is arranged between the center hole and the opening in the bottom of the shell, and the liquid delivery pipe penetrates through the guide pipe and then extends out of the shell.

Furthermore, a pressing plate with the diameter larger than that of the counting button is arranged at the bottom of the touch and press rod.

Has the advantages that:

1. the liquid transfer device suitable for continuous sample adding disclosed by the invention is simple in structure, and mainly aims at carrying out multi-hole successive sample adding design on a 96-hole groove plate which is a structure device with a plurality of holes, the sample adding operation is convenient, the error rate can be obviously reduced, the problem that repeated sample adding is started due to errors is avoided, the experimental materials are saved, the time cost of operators is reduced, and the physical burden of the operators is lightened;

2. the design of the sample feeding assembly enables the sample feeding process to be continuously operated without repeatedly sucking samples, so that the working process is accelerated, and the continuous sample feeding can be realized, so that the sequential operation process cannot be interrupted due to sample sucking, and the error rate in the sample feeding process can be obviously reduced;

3. the counting assembly is designed in a pure mechanical mode, the design cost is low, the damage rate is low compared with that of an electric counter, and the service life of the whole device is prolonged;

4. two counting disks in the counter are specially designed for the loading operation of the 96-hole slot plate, the characters passing through the observation display window in the operation process can correspond to corresponding coordinates on the 96-hole slot plate, even if the loading position is forgotten due to distraction or external interference in the loading process, the correct position can be quickly found through the window characters for subsequent operation, and the error rate of the operation process is greatly reduced.

Drawings

FIG. 1 is a schematic front view of a pipetting device suitable for continuous sample application;

FIG. 2 is a sectional view showing the internal structure of a pipetting device suitable for continuous sample application;

FIG. 3 is an enlarged view of portion B of FIG. 1;

FIG. 4 is a schematic diagram of the internal structure of the counter;

FIG. 5 is a schematic view of the engagement of the internal ratchet and the pawl;

FIG. 6 is a schematic structural view of a transmission mechanism of the counting plate;

FIG. 7 is a schematic view showing an assembled and coupled structure of the counter plate portion;

FIG. 8 is an exploded view of the construction of the counter plate portion;

FIG. 9 is a schematic view of the position arrangement of the rack and the return spring;

the device comprises a shell, a piston assembly, a pushing device, a sample injection assembly and a counting assembly, wherein the shell is 1, the piston assembly is 2, the pushing device is 3, and the counting assembly is 5;

11-upper shell, 12-lower shell, 13-middle platform, 14-sample tube socket, 15-guide tube;

21-push button, 22-piston rod, 23-upper boss, 24-lower boss and 25-reset spring;

31-a push rod;

41-sample tube, 42-liquid outlet pump, 43-liquid outlet tube, 44-sample tube cover body;

51-counter, 52-touch-press component;

511-housing, 512-display window, 513-count button, 514-reset knob, 515-first count disk, 516-second count disk;

521-sleeve, 522-touch rod, 523-return spring, 524-pressing disk;

01-central shaft, 02-positioning disk, 03-positioning pin, 04-gear, 05-rack, 06-return spring, 07-internal ratchet, 08-pawl, 09-positioning piece, 010-notch, 011-positioning shaft, 012-pinion and 013-insection.

Detailed Description

The following detailed description of the preferred embodiments of the present invention is provided to enable those skilled in the art to more readily understand the advantages and features of the present invention, and to clearly and unequivocally define the scope of the present invention.

A sample plate with 96 holes is commonly used in a laboratory and a systematic cultivation process, and a laboratory 96-hole plate is small in specification and mainly used for large-batch test experiments; the 96-hole trough plate for cultivation has larger specification, can be used for the seedling cultivation process, is used for researching the influence of different nutrient elements on the growth condition of seedlings to cultivate high-quality plant varieties, and after planning, liquid culture agents containing different elements are often required to be added into different holes on the plate to carry out big data contrast analysis to observe the influence on the plants in the cultivation process (firstly, reagents 1 are required to be added into 96 empty grooves, then, reagents 2 are all added into the first to fourth rows of holes, reagents 3 are all added into the fifth to eighth rows of holes to research the influence of the

reagents

2 and 3 on the reagents 1 under the condition that the reagents 1 exist, and the like), no matter the 96-hole plate is used for small-sized laboratories or the 96-hole trough plate is used for cultivation, in order to improve the accuracy rate of operation when liquid sample adding is carried out on the 96-hole trough plate, sample adding holes can be positioned at any time, thereby ensuring no leakage or no heavy addition, avoid leading to the follow-up result to appear unnecessary problem because of misoperation, influence the strive nature of research conclusion, provide a move liquid device suitable for continuous application of sample in this embodiment, should move liquid device and include casing 1, establish piston assembly 2, pusher 3 and the kind subassembly 4 in casing 1 to and establish the counting assembly 5 outside casing 1.

The piston assembly 2 comprises a piston rod 22 of which the top is fixedly connected with a push button 21, an upper boss 23 is arranged at the lower part of the piston rod 22, a lower boss 24 is fixedly connected in the shell 1 below the upper boss 23, the bottom end of the piston rod 22 penetrates through the lower boss 24 to be connected with the pushing device 3, and a return spring 25 is arranged on the piston rod 22 between the upper boss and the lower boss;

the sample feeding assembly 4 is arranged below the pushing device 3, the sample feeding assembly 4 comprises a sample tube 41, a liquid outlet pump 42 and a liquid delivery tube 43, the liquid delivery tube 43 is communicated with a pump port of the liquid outlet pump 42, the liquid delivery tube 43 and the liquid outlet pump 42 are integrally arranged, the liquid delivery tube 43 vertically extends out of the shell 1, liquid pumped out of the pump port of the liquid outlet pump 42 can directly flow out of the gun body along the liquid delivery tube 43, the liquid outlet pump 42 adopts the structure of the existing liquid delivery pump, which is not described herein in much detail, the liquid outlet pump 42 is fixedly connected to a sample tube cover body 44, the sample tube cover body 44 is connected with the top of the sample tube 41 in an inserting manner, and the top of the liquid outlet pump 42 is detachably and fixedly connected with the pushing rod 31 at the bottom of the pushing device;

in order to facilitate subsequent disassembly and replacement, the top of the liquid outlet pump 42 is fixedly connected with the pushing rod 31 in an inserted manner.

Casing 1 includes casing 11 and casing 12 down, go up casing 11 and casing 12 plug-in connection down, be equipped with middle platform 13 in casing 12 down, be equipped with sample cell socket 14 on middle platform 13, be equipped with the spliced eye that adapts to sample cell 41 bottom shape in sample cell socket 14 middle part, sample cell 41 bottom can be pegged graft and fix in the spliced eye, be equipped with the centre bore in middle platform 13 middle part, set up stand pipe 15 from centre bore to casing 1 bottom opening within a definite time, liquid contact tube 43 stretches out to casing 1 outside after passing stand pipe 15.

The counting assembly 5 comprises a counter 51 and a touch assembly 52, the counter 51 is fixed on the shell 1, the touch assembly 52 is connected on the shell 1, the counter 51 comprises a shell 511 and a counting disc sequence consisting of two counting discs, a display window 512 for displaying the numbers on the counting disc sequence is arranged on the shell 511, a counting button 513 is arranged at the top of the shell 511 and below the touch assembly 52, and a reset knob 514 is arranged at the right side of the counter 51;

since the pipetting device disclosed in this embodiment is designed mainly for loading a 96-well plate, eight letters a-H can be marked longitudinally on the left side of the 96-well plate, several numbers 1-12 are marked transversely on the top, and each well has a specific coordinate, in this embodiment, for better corresponding counting, numbers 0 to 12 are marked on the outer circumference of the first counting plate 515 at predetermined angles, the second counting plate 516 is arranged adjacent to the first counting plate 515 along the same axis, and letters a-H are marked on the outer circumference of the second counting plate 516 at predetermined angles, after pressing the counting button 51312 times to make the first counting plate 515 rotate a complete turn, the second counting plate 516 rotates a part of the counting plate at a predetermined angle and rotates from the previous letter to the next letter in the rotating direction, such design can accurately load the 96-well plate, even if the operator appears tiredly because of frequent operation and shakes the spirit, the distraction or leads to forgetting to which sample adding hole of oneself because of other external disturbances in the application of sample in-process, only need to see the count number on the counter and confirm the application of sample coordinate, later find on the 96 hole frid corresponding coordinate add sample hole after then in order the application of sample once more can, the error rate in the reducible a large amount of application of sample experimentation of this design avoids causing harmful effects to the experimental result because of the unnecessary problem.

The touch pressure assembly 52 comprises a sleeve 521 and a touch pressure rod 522, the sleeve 521 is fixedly connected to the housing 1, the touch pressure rod 522 is movably sleeved in the sleeve 521, the diameter of the touch pressure rod 522 is matched with the inner diameter of the sleeve 521, and a return spring 523 is arranged between the bottom of a top disk of the touch pressure rod and the top surface of the sleeve 521.

In order to improve the accuracy of the contact pressing process, a pressing plate 524 with a diameter larger than that of the counting button is arranged at the bottom of the contact pressing rod 522.

Except for the problem of the system, the internal dial ring coupling structure of the counter is not improved, and the internal coupling structure of the existing proper mold counter can be applied to the counter only by correspondingly adjusting the problem of the system.

Here, a counter coupling structure of a transmission structure is specifically taken as an example for simple description: the shell 511 of the counter 51 is formed by riveting and connecting two parts, a central shaft 01 is arranged in the shell, positioning disks 02 corresponding to the counting disks are sleeved on the central shaft 01, each positioning disk 02 is positioned on the inner side of the corresponding counting disk, each positioning disk 02 is connected by a positioning pin 03, and the positioning pins 03 and the positioning disks 02 are integrally arranged; one end of the central shaft 01 is fixedly connected with a gear 04, the gear 04 is meshed with a rack 05, the lower end of the rack 05 is provided with a return spring 06, the bottom of the return spring 06 is fixed on the outer shell 511, the upper end of the rack 05 extends out of the outer shell 511 to form a counting button 513, the surface of the first counting disc 515 facing the gear 04 is provided with a ratchet surface, the edge of the gear 04 is provided with a pawl 08 matched with an inner ratchet 07 of the ratchet surface, and the gear 04 can be an incomplete gear.

The end of center pin 01 is equipped with spacer 09, the end and the spacer 09 fixed connection of locating pin 03, the front end and the gear 04 fixed connection of locating pin 03, is equipped with breach 010 on the positioning disk 02, is equipped with the location axle 011 in the breach 010, and the cover is equipped with pinion 012 on the location axle 011, and the inboard of counting disk is equipped with the insection 013 with pinion 012 complex, and the center pin 01 drives the counting disk rotation by pinion 012 when rotating.

The reset knob 514 is connected with the central shaft 01, and the structural arrangement of the reset knob 514 is the same as that of a reset knob on a traditional counter, so that the reset knob mainly plays a role in resetting and zeroing, and redundant description is not repeated herein.

The specific application method of the liquid transfer device is as follows:

when the device is used, firstly, a liquid sample (which is more than required amount and needs to be debugged) is prepared and filled into a clean sample tube 41, a sample tube cover 44 is fixed at the top, the lower shell 12 is pulled out from the upper shell 11, the liquid delivery tube 43 is inserted into the guide tube 15, the bottom of the sample tube 41 is connected to the sample tube socket 14 in a clamping manner, then the lower shell 12 is connected to the upper shell 11 in a re-inserting manner, and the push rod 31 is just inserted into the top of the liquid outlet pump 42 after the insertion is completed;

then, press push button 21 several times at first, push button 21 pushes down and drives pusher 3 to push down, and then drives out liquid pump 42 to push down, and the reagent in sample tube 41 is extruded, because there is not reagent in liquid delivery tube 43 at the beginning, so direct sample loading can lead to the sample volume inaccurate, so press push button 21 several times before formal sample loading first, until there is reagent outflow in liquid delivery tube 43 that can prove that has filled in liquid delivery tube 43 with reagent, can direct sample loading when pressing next time.

Then, after determining that the counting disc sequence of the counter 51 is in the initial state (i.e. 1A is visible from the display window 512), the sample adding operation can be formally started, the push button 21 is pressed once, while the hydraulic delivery tube 43 is normally sampling, the touch assembly 52 synchronously presses the counting button 513 (i.e. the rack 05), when the rack 05 is pressed down, the rack 05 drives the gear 04 to rotate, the pawl 08 on the gear 04 is matched with the ratchet surface to drive the first counting disc 515 to rotate by a preset angle, the push button 21 is released, the touch rod 522 returns to the original position under the action of the return spring 523, the rack 05 returns to the original position under the action of the return spring 06, and when the rack 12 is pressed down to make the first counting disc 515 rotate by a complete circle, the second counting disc 516 rotates by a part of the preset angle, so as to count the sample adding of the well plate 96.

And because this design can realize going out the appearance in succession, need not midway to inhale the appearance repeatedly, the operation process is more smooth, and incessant operation midway can reduce the application of sample error rate, further guarantees the accuracy of application of sample operation.

After the sample adding is finished, the device is assembled after the sample tube 41 is filled with the washing reagent, the pressing and pushing unit 21 can utilize the washing reagent to wash the reagent in the liquid delivery tube 43 and the liquid outlet pump 42, and the washing can be finished for the next use.

During routine operation, the volume of carrying out different liquid sample application of sample probably has the difference, this device of multiple specification can be generally dispose simultaneously in order to be applicable to the interpolation of different samples, because the workflow that involves when the people in corresponding field carries out a series of 96-well cell board application of sample operation all is fixed, limited mostly, so even to the device of a specification of a kind of volume customization of advancing, the device quantity also can not be too many, use cost is not very high yet, the device mainly wants the problem of the continuous application of sample that only solves.

However, in view of improving the applicability, the applicant does not limit the addition of a sample amount control component in the device to adjust the sample amount, so that a set of device is suitable for adding different amounts of different liquids, and the conventional method for controlling the sample amount can be realized from multiple aspects, such as using a liquid outlet pump structure with adjustable sample amount, or using a piston component (similar to the structure on the liquid-moving gun for the existing laboratory), since these designs are already available in the prior art, and the specific structure is not described herein in a limited way, so long as the structure is suitable, but simply adding such an existing design to the device should be included in the protection scope of the present invention, and should not be regarded as a limitation to the technical scheme of the present invention.

The above description is only an embodiment of the present invention, and not intended to limit the scope of the present invention, and all modifications of equivalent structures and equivalent processes performed by the present specification and drawings, or directly or indirectly applied to other related technical fields, are included in the scope of the present invention.

Claims

1. A liquid transfer device suitable for continuous sample adding is characterized by comprising a shell (1), a piston assembly (2), a pushing device (3) and a sample injection assembly (4) which are arranged in the shell (1) in sequence from top to bottom, and a counting assembly (5) arranged outside the shell (1);

the sample feeding assembly (4) is arranged below the pushing device (3), the sample feeding assembly (4) comprises a sample tube (41), a liquid outlet pump (42) and a liquid outlet tube (43), the liquid outlet tube (43) is communicated with a pump port of the liquid outlet pump (42) and integrally arranged with the liquid outlet pump, the liquid outlet tube (43) vertically extends out of the shell (1), the liquid outlet pump (42) is fixedly connected to a sample tube cover body (44), the sample tube cover body (44) is in plug-in connection with the top of the sample tube (41), and the top of the liquid outlet pump (42) is detachably and fixedly connected with a pushing rod (31) at the bottom of the pushing device (3);

counting assembly (5) include counter (51) and touch subassembly (52), counter (51) are fixed on casing (1), touch subassembly (52) and connect on shell (1), counter (51) are including shell (511) and the counting dish sequence that two counting dishes are constituteed, be equipped with display window (512) on shell (511), shell (511) top just is located the below that touches subassembly (52) and is equipped with counting button (513), shell (511) right side is equipped with reset knob (514).

2. A pipette device suitable for continuous loading according to claim 1, characterized in that the counting disc sequence is composed of a first counting disc (515) and a second counting disc (516), the numbers 0 to 12 are marked on the outer circumference of the first counting disc (515) at predetermined angles, the second counting disc (516) is arranged adjacent to the first counting disc (515) along the same axis, and the letters a to H are marked on the outer circumference of the second counting disc (516) at predetermined angles, the counting button (513) is pressed 12 times, the first counting disc (515) is rotated by a complete turn by a toggle, and the second counting disc (516) rotates the part by a predetermined angle and clockwise from the previous letter to the next letter.

3. A liquid-transfering device suitable for continuous sample application according to claim 1, characterized in that, the piston assembly (2) includes a piston rod (22) with a push button (21) fixed on the top, an upper boss (23) is provided on the lower portion of the piston rod (22), a lower boss (24) is fixedly connected in the casing below the upper boss (23), the bottom end of the piston rod (22) passes through the lower boss (24) to connect with the pushing device (3), and a return spring (25) is provided on the piston rod (22) between the upper and lower bosses.

4. A liquid-transfering device suitable for continuous sample application according to claim 1, characterised in that the top of the liquid-discharging pump (42) is fixedly connected with the pushing rod (31) in a plugging manner.

5. A liquid-transfering device suitable for continuous sample application according to claim 1, characterized in that, the casing (1) includes an upper casing (11) and a lower casing (12), the upper casing (11) is connected with the lower casing (12) in an inserting manner, a middle platform (13) is arranged in the lower casing (12), a sample tube socket (14) is arranged on the middle platform (13), an inserting hole adapted to the shape of the bottom of the sample tube (41) is arranged in the middle of the sample tube socket (14), and the bottom of the sample tube (41) is inserted and fixed in the inserting hole.

6. The liquid-transfering device suitable for continuous sample application according to claim 1, characterized in that, the touch-press component (52) includes a sleeve (521) and a touch-press rod (522), the sleeve (521) is fixedly connected to the casing (1), the touch-press rod (522) is movably sleeved in the sleeve (521), the diameter of the touch-press rod (522) is adapted to the inner diameter of the sleeve (521), and a return spring (523) is arranged between the top disk bottom of the touch-press rod and the top surface of the sleeve (521).

7. A pipetting device for continuous sample application according to claim 5 characterized in that the intermediate platform (13) is provided with a central hole in the middle, a guide tube (15) is arranged from the central hole to the bottom opening of the housing (1), and the liquid outlet tube (43) extends through the guide tube (15) to the outside of the housing (1).

8. A pipetting device for continuous sample application according to claim 6 characterized in that a pressing plate (524) with a diameter larger than the diameter of the counting button (513) is provided at the bottom of the contact lever (522).