CN112403547B

CN112403547B - Pipetting device suitable for continuous sample addition

Info

Publication number: CN112403547B
Application number: CN202011498341.8A
Authority: CN
Inventors: 梅丛进; 余传信; 杨莹莹; 宋丽君; 董盼盼; 周永华; 于汉寿
Original assignee: Jiangsu Institute of Parasitic Diseases
Current assignee: Jiangsu Institute of Parasitic Diseases
Priority date: 2020-12-17
Filing date: 2020-12-17
Publication date: 2023-04-28
Anticipated expiration: 2040-12-17
Also published as: CN112403547A

Abstract

The invention discloses a pipetting device suitable for continuous sample addition, which comprises a shell, a piston assembly, a pushing device, a sample injection assembly and a counting assembly, wherein the piston assembly, the pushing device and the sample injection assembly are arranged in the shell; the sample injection assembly comprises a sample tube, a liquid outlet pump and a liquid delivery tube, the liquid delivery tube is in through connection with a liquid outlet pump port, the liquid delivery tube vertically extends out of the shell, the liquid outlet pump is connected to a sample tube cover body, and the top of the liquid outlet pump is connected with the pushing rod; the counter assembly comprises a counter and a touch-press assembly, the counter is fixed on the shell, the touch-press assembly is connected to the shell, the counter comprises a shell and a counting disc sequence consisting of two counting discs, a display window is arranged on the shell, a counting button is arranged at the top of the shell, and a reset knob is arranged on the right side of the shell. The pipetting device is designed mainly for the 96-well slot plate sample adding process, so that the error rate can be remarkably reduced, and the accuracy of an operation result is ensured.

Description

Pipetting device suitable for continuous sample addition

Technical Field

The invention relates to the field of sample adding devices, in particular to a pipetting device suitable for continuous sample adding.

Background

The common pipetting device usually finishes one sample loading after one sample loading, the sample loading process is discontinuous, when the whole-hole sequential sample loading operation is required to be carried out on a slot plate structure with 96 slots, namely a slot plate structure with more slots, the process is repeated for a plurality of times, the sample loading quantity is large and the repeatability is high, operators often forget the specific slot positions where the sample loading is finished during the continuous sample loading and the sample loading process, particularly, if the prior sample or other contents exist in the slot, the sample loading position is difficult to judge simply from whether the slot is empty or not, the sample loading times are easy to forget (or misrecord) once the sample loading process is interrupted by other matters or other personnel during the sample loading, the operation result is influenced, and even the sample loading operation is required to be carried out again by giving up the sample loading process, so that the waste of materials is caused, and the labor of the operators is consumed too much.

Disclosure of Invention

The invention aims to provide a pipetting device which is reasonable in structural design and suitable for continuous sample addition.

The invention is realized in the following way: the device comprises a shell, a piston assembly, a pushing device, a sample injection assembly and a counting assembly, wherein the piston assembly, the pushing device and the sample injection assembly are arranged in the shell from top to bottom in sequence; the sample injection assembly is arranged below the pushing device and comprises a sample tube, a liquid outlet pump and a liquid delivery tube, the liquid delivery tube is in through connection with a liquid outlet pump opening and is integrally arranged with the liquid delivery tube, the liquid delivery tube vertically extends out of the shell, the liquid outlet pump is fixedly connected to a sample tube cover body, the sample tube cover body is in plug connection with the top of the sample tube, and the top of the liquid outlet pump is detachably and fixedly connected with a pushing rod at the bottom of the pushing device; the counter assembly comprises a counter and a touch assembly, the counter is fixed on the shell, the touch assembly is connected to the shell, the counter comprises a shell and a counting disc sequence consisting of two counting discs, a display window is arranged on the shell, a counting button is arranged at the top of the shell and below the touch assembly, and a reset knob is arranged on the right side of the shell.

Further, the counting disc sequence is composed of a first counting disc, on the outer circumference of which numerals 0 to 12 are marked at a predetermined angle, and a second counting disc, which is arranged adjacent to the first counting disc along the same axis and on the outer circumference of which letters a to H are marked at a predetermined angle, pressing the counting button 12 times, the first counting disc toggles a complete turn and the second counting disc turns a part of the turn by a predetermined angle and clockwise from the last letter to the next letter.

Further, the piston assembly comprises a piston rod, the top of the piston rod is fixedly connected with a pushing button, an upper boss is arranged at the lower part of the piston rod, a lower boss is fixedly connected in a shell below the upper boss, the bottom end of the piston rod penetrates through the lower boss to be connected with the pushing device, and a reset spring is arranged on the piston rod between the upper boss and the lower boss.

Further, the top of the liquid outlet pump is fixedly connected with the pushing rod in an inserting way.

Further, the casing includes casing and lower casing, goes up casing and lower casing grafting and is connected, is equipped with the intermediate platform in the lower casing, is equipped with the sample tube socket on the intermediate platform, is equipped with the spliced eye with sample tube bottom shape adaptation in the middle part of the sample tube socket, and the sample tube bottom is pegged graft and is fixed in the spliced eye.

Further, touch the subassembly and include sleeve and touch the depression bar, sleeve fixed connection is on the casing, touches the depression bar activity and cup joints in the sleeve, touches the diameter of depression bar and telescopic internal diameter size and suits, is equipped with return spring between the top disc bottom of touching the depression bar and sleeve top surface.

Further, a central hole is formed in the middle of the middle platform, a guide pipe is arranged between the central hole and an opening at the bottom of the shell, and the liquid delivery pipe penetrates through the guide pipe and then extends out of the shell.

Further, a pressing disc with the diameter larger than that of the counting button is arranged at the bottom of the touch pressing rod.

The beneficial effects are that:

1. the pipetting device suitable for continuous sample loading disclosed by the invention is simple in structure, is mainly designed for carrying out porous successive sample loading on a structural device with a plurality of holes and slots, such as a 96-hole slot plate, is convenient to sample loading operation, can remarkably reduce error rate, avoids the problem that repeated sample loading is required due to error, saves experimental materials, reduces time cost of operators and lightens physical burden of the operators;

2. the design of the sample feeding assembly enables the sample feeding process to be operated continuously, repeated sample suction is not needed, the working process is accelerated, and the sequential operation process is not broken due to sample suction as continuous sample feeding can be realized, so that the error rate in the sample feeding process can be obviously reduced;

3. the counting assembly is of a purely mechanical design, the design cost is low, compared with an electric counter, the damage rate is small, and the whole service life of the equipment is prolonged;

4. the two counting discs in the counter are designed specially for 96-hole slot plate sample adding operation, characters of the display window can be observed to correspond to corresponding coordinates on the 96-hole slot plate in the operation process, even if the sample adding position is forgotten due to distraction or external interference in the sample adding process, the correct position can be quickly found through the window characters to carry out subsequent operation, and the error rate in the operation process is greatly reduced.

Drawings

FIG. 1 is a schematic front view of a pipetting device suitable for continuous sample application;

FIG. 2 is a cross-sectional view showing the internal structure of a pipetting device adapted for continuous sample application;

FIG. 3 is an enlarged view of portion B of FIG. 1;

FIG. 4 is a schematic diagram of the internal structure of the counter;

FIG. 5 is a schematic view of the engagement of the inner ratchet teeth with the pawl;

FIG. 6 is a schematic diagram of a counting disk drive mechanism;

FIG. 7 is a schematic diagram of an assembled connection structure of the counting disk section;

FIG. 8 is an exploded view of a portion of the structure of the counting disc;

FIG. 9 is a schematic view of the positioning of the rack and return spring;

the device comprises a 1-shell, a 2-piston assembly, a 3-pushing device, a 4-sample injection assembly and a 5-counting assembly, wherein the shell is provided with a plurality of holes;

11-upper shell, 12-lower shell, 13-middle platform, 14-sample tube socket and 15-guide tube;

21-push button, 22-piston rod, 23-upper boss, 24-lower boss and 25-return spring;

31-pushing rod;

41-sample tube, 42-liquid outlet pump, 43-liquid delivery tube, 44-sample tube cover;

51-counter, 52-touch-press assembly;

511-housing, 512-display window, 513-count button, 514-reset knob, 515-first count disk, 516-second count disk;

521-sleeve, 522-contact lever, 523-return spring, 524-pressing plate;

the device comprises a central shaft 01-02-positioning disc, a 03-positioning pin, a 04-gear, a 05-rack, a 06-return spring, 07-inner ratchets, a 08-pawl, 09-positioning sheets, 010-notches, 011-positioning shafts, 012-pinions and 013-insections.

Detailed Description

The following detailed description of the preferred embodiments of the invention is provided to enable those skilled in the art to more readily understand the advantages and features of the invention and to make a clear and concise definition of the scope of the invention.

In the laboratory and systematic cultivation process, a sample plate with 96 holes is often used, and the laboratory 96 holes have small specification and are mainly used for large-batch test experiments; the specification of 96-hole slot plates for cultivation is larger, can be used for seedling cultivation process, in order to study the influence of different nutrient elements on seedling growth conditions, to cultivate high-quality plant varieties, after planning, often need add the liquid culture agent that contains different elements in the different hole slots on the board in cultivation process and do big data contrast analysis and observe its influence to the plant (at first need all add reagent 1 in 96 empty slots, afterwards first to four rows of hole slots add reagent 2, the whole reagent 3 that adds of fifth to eight rows of hole slots is used for studying under the condition that there is reagent 1, such research as the influence of

reagent

2 and 3 to reagent 1, whether small-size 96-hole slot plates for laboratory use or cultivation are used for 96-hole slot plates of big specification, in order to improve the correct rate of operation when carrying out the liquid sample on 96-hole slot plates, can fix a sample adding hole at any time, and guarantee that the leak-free does not add again, avoid the unnecessary problem that the follow-up result appears because of the operation error, influence research's the availability, provide a pipetting device suitable for continuous pipetting device in this embodiment, the pipetting device is provided in this embodiment, the housing 1 is arranged in the housing, the housing 1 is arranged in the case, the housing 1, the housing is provided with the 5, the housing 1, the housing is provided with the piston assembly, the 5, the housing 1 is arranged outside the housing 1, and the housing 1, the 5 is in the counter assembly.

The piston assembly 2 comprises a piston rod 22, the top of which is fixedly connected with a push button 21, an upper boss 23 is arranged at the lower part of the piston rod 22, a lower boss 24 is fixedly connected in the shell 1 positioned below the upper boss 23, the bottom end of the piston rod 22 passes through the lower boss 24 to be connected with the push device 3, and a return spring 25 is arranged on the piston rod 22 positioned between the upper boss and the lower boss;

the sample injection assembly 4 is arranged below the pushing device 3, the sample injection assembly 4 comprises a sample tube 41, a liquid outlet pump 42 and a liquid outlet pipe 43, the liquid outlet pipe 43 is communicated with a pump port of the liquid outlet pump 42, the liquid outlet pipe 43 and the liquid outlet pump 42 are integrally arranged, the liquid outlet pipe 43 vertically extends out of the shell 1, liquid pumped by a pump port of the liquid outlet pump 42 can directly flow out of the gun body along the liquid outlet pipe 43, the liquid outlet pump 42 adopts the structure of the existing liquid outlet pump, the excessive description is omitted, the liquid outlet pump 42 is fixedly connected to a sample tube cover 44, the sample tube cover 44 is connected with the top of the sample tube 41 in a plugging manner, and the top of the liquid outlet pump 42 is detachably and fixedly connected with a pushing rod 31 at the bottom of the pushing device 3;

to facilitate the subsequent disassembly and replacement, the top of the liquid outlet pump 42 is fixedly connected with the pushing rod 31 in a plugging manner.

The shell 1 comprises an upper shell 11 and a lower shell 12, the upper shell 11 is in plug connection with the lower shell 12, a middle platform 13 is arranged in the lower shell 12, a sample tube socket 14 is arranged on the middle platform 13, a plug hole which is adaptive to the shape of the bottom of the sample tube 41 is formed in the middle of the sample tube socket 14, the bottom of the sample tube 41 can be fixed in the plug hole in a plug manner, a central hole is formed in the middle of the middle platform 13, a guide tube 15 is arranged between the central hole and an opening at the bottom of the shell 1, and a liquid delivery tube 43 penetrates through the guide tube 15 and then extends out of the shell 1.

The counting assembly 5 comprises a counter 51 and a touch-press assembly 52, the counter 51 is fixed on the shell 1, the touch-press assembly 52 is connected to the shell 1, the counter 51 comprises a shell 511 and a counting disc sequence formed by two counting discs, a display window 512 for displaying numbers on the counting disc sequence is arranged on the shell 511, a counting button 513 is arranged at the top of the shell 511 and below the touch-press assembly 52, and a reset knob 514 is arranged on the right side of the counter 51;

because the pipetting device disclosed in this embodiment is designed mainly for 96-well plate sample application, the left side of the 96-well plate is vertically marked with eight letters a-H, the top is horizontally marked with several numbers 1-12, and each slot hole has a specific coordinate, so in this embodiment, in order to better perform corresponding counting, the number 0 to 12 is marked on the outer circumference of the first counting plate 515 at a predetermined angle, the second counting plate 516 is arranged along the same axis adjacent to the first counting plate 515, letters a-H are marked on the outer circumference of the second counting plate 516 at a predetermined angle, after the first counting plate 515 is shifted by a circle of rotation by pressing the counting button 513 12 times, the second counting plate 516 rotates a part of the rotation by a predetermined angle and changes the rotation direction from the last letter to the next letter, so that the design can accurately apply samples to the 96-well plate sample, even if an operator forgets to apply the corresponding number to the corresponding to the counting plate sample application by frequently operating, is distracted or forgets to the operator because of other operations, the number is not needed to be applied to the corresponding to the number of the counter, and the number is not needed to be reduced, and then the experiment error is avoided after the experiment is repeated after the experiment is performed to determine the number that the number is needed to apply the corresponding to the number to the well sample application, and the sample application is not needed.

The touch assembly 52 comprises a sleeve 521 and a touch bar 522, the sleeve 521 is fixedly connected to the housing 1, the touch bar 522 is movably sleeved in the sleeve 521, the diameter of the touch bar 522 is adapted to the inner diameter of the sleeve 521, and a return spring 523 is arranged between the bottom of the top disc of the touch bar and the top surface of the sleeve 521.

To improve the accuracy of the contact pressing process, a pressing plate 524 with a diameter larger than that of the count button is provided at the bottom of the pressing lever 522.

Besides the problem of the system, the internal dial ring coupling structure of the counter is not improved, and the internal coupling structure of the existing proper die counter can be suitable for the internal dial ring coupling structure of the counter, so long as the problem of the system is correspondingly adjusted.

A counter coupling structure of a transmission structure is specifically described herein as an example for simplicity: the shell 511 of the counter 51 is formed by riveting two parts, a central shaft 01 is arranged in the shell, positioning disks 02 corresponding to the counting disks are sleeved on the central shaft 01, each positioning disk 02 is positioned on the inner side of the corresponding counting disk, each positioning disk 02 is connected by a positioning pin 03, and the positioning pin 03 and the positioning disk 02 are integrally arranged; the gear 04 is fixedly connected to one end of the central shaft 01, the gear 04 is meshed with a rack 05, the lower end of the rack 05 is provided with a return spring 06, the bottom of the return spring 06 is fixed on the shell 511, the upper end of the rack 05 extends out of the shell 511 to form a counting button 513, a ratchet surface is arranged on the surface of the first counting disc 515 facing the gear 04, a pawl 08 matched with an inner ratchet 07 of the ratchet surface is arranged on the edge of the gear 04, and the gear 04 is an incomplete gear.

The end of the central shaft 01 is provided with a locating plate 09, the end of the locating pin 03 is fixedly connected with the locating plate 09, the front end of the locating pin 03 is fixedly connected with the gear 04, a notch 010 is arranged on the locating plate 02, a locating shaft 011 is arranged in the notch 010, a pinion 012 is sleeved on the locating shaft 011, the inner side of the counting plate is provided with a tooth 013 matched with the pinion 012, and the counting plate is driven to rotate by the pinion 012 when the central shaft 01 rotates.

The reset knob 514 is connected with the central shaft 01, and the structure of the reset knob 514 is the same as that of the reset knob on the traditional counter, mainly plays a role in resetting and zeroing, and is not repeated here.

The specific use method of the pipetting device is as follows:

when in use, firstly, a liquid sample is prepared (more than required amount is needed because debugging is needed), the liquid sample is filled into a clean sample tube 41, a sample tube cover 44 is fixed at the top, the lower shell 12 is pulled out from the upper shell 11, a liquid delivery tube 43 is penetrated into the guide tube 15, the bottom of the sample tube 41 is connected to the sample tube socket 14 in a clamping way, then the lower shell 12 is connected to the upper shell 11 in a plugging way again, and the push rod 31 is just plugged at the top of the liquid outlet pump 42 after plugging is finished;

after that, the push button 21 is pushed for several times, the push button 21 is pushed down to drive the push device 3 to push down, and then the liquid outlet pump 42 is driven to push down, the reagent in the sample tube 41 is extruded, and the sample injection amount is inaccurate due to the fact that no reagent exists in the liquid delivery tube 43 at the beginning, so that the push button 21 is pushed for several times before formal sample injection is performed until the reagent flows out from the bottom of the liquid delivery tube 43, the reagent in the liquid delivery tube 43 can be proved to be filled, and the sample can be directly injected when the reagent is pressed next time.

Then, after determining that the counting disc sequence of the counter 51 is in the initial state (i.e. 1A can be seen from the display window 512), the sample adding operation can be formally started, when the push button 21 is pushed once, the push assembly 52 synchronously pushes the counting button 513 (i.e. the rack 05) while the liquid delivery tube 43 normally outputs samples, when the rack 05 is pushed down, the rack 05 drives the gear 04 to rotate, the pawl 08 on the gear 04 cooperates with the ratchet surface to drive the first counting disc 515 to rotate by a preset angle, the push button 21 is released, the push rod 522 returns to the original position under the action of the return spring 523, the rack 05 returns to the original position under the action of the return spring 06, and when the rack 12 is pushed down to enable the first counting disc 515 to rotate for a complete circle, the second counting disc 516 rotates by a preset angle, so that the sample adding of the 96-well plate can be counted.

And because the design can realize continuous sample discharge, repeated sample suction in the middle is not needed, the operation process is smoother, and the sample feeding error rate can be reduced by uninterrupted operation in the middle, so that the accuracy of sample feeding operation is further ensured.

After the sample is added, the device is assembled after the flushing reagent is filled in the sample tube 41, and the flushing reagent can be used for flushing the reagent in the liquid delivery tube 43 and the liquid outlet pump 42 by pressing the push button 21, so that the flushing is finished and the device can be used for the next time.

In normal operation, the sample adding amounts of different liquid samples may be different, and the device with multiple specifications can be configured at the same time to be suitable for adding different samples, because most of the working processes involved in the serial 96-well plate sample adding operation by people in the corresponding field are fixed and limited, even if the device with one specification is customized for one sample adding amount, the device number is not too, the use cost is not very high, and the device mainly aims to solve the problem of uninterrupted continuous sample adding.

However, in view of improving applicability, the applicant does not limit the addition of the sample amount control assembly to the device to adjust the sample amount, so that a set of devices is suitable for adding different amounts of different liquids, and the conventional method of controlling the sample amount can be implemented in various aspects, such as using a liquid outlet pump structure with adjustable sample amount or using a piston assembly (similar to a structure on an existing laboratory pipette) with screwing adjustment, since the designs have already been presented in the prior art, specific structures are not limited herein, and any suitable structure is applicable, but simply adding such existing designs to the device should not be considered as limiting the technical scheme of the invention.

The foregoing description is only illustrative of the present invention and is not intended to limit the scope of the invention, and all equivalent structures or equivalent processes or direct or indirect application in other related technical fields are included in the scope of the present invention.

Claims

1. The pipetting device suitable for continuous sample addition is characterized by comprising a shell (1), a piston assembly (2), a pushing device (3) and a sample injection assembly (4) which are arranged in the shell (1) from top to bottom in sequence, and a counting assembly (5) arranged outside the shell (1);

the sample injection assembly (4) is arranged below the pushing device (3), the sample injection assembly (4) comprises a sample tube (41), a liquid outlet pump (42) and a liquid delivery tube (43), the liquid delivery tube (43) is in through connection with a pump port of the liquid outlet pump (42) and is integrally arranged with the liquid delivery tube, the liquid delivery tube (43) vertically extends out of the shell (1), the liquid outlet pump (42) is fixedly connected to a sample tube cover body (44), the sample tube cover body (44) is connected with the top of the sample tube (41) in a plug-in connection mode, and the top of the liquid outlet pump (42) is detachably and fixedly connected with a pushing rod (31) at the bottom of the pushing device (3);

the counting assembly (5) comprises a counter (51) and a touch-press assembly (52), the counter (51) is fixed on the shell (1), the touch-press assembly (52) is connected to the shell (1), the counter (51) comprises a shell (511) and a counting disc sequence consisting of two counting discs, a display window (512) is arranged on the shell (511), a counting button (513) is arranged at the top of the shell (511) and below the touch-press assembly (52), and a reset knob (514) is arranged on the right side of the shell (511);

the counting disc sequence consists of a first counting disc (515) and a second counting disc (516), numbers 0 to 12 are marked on the outer circumference of the first counting disc (515) at a preset angle, the second counting disc (516) is arranged adjacent to the first counting disc (515) along the same axis, letters A-H are marked on the outer circumference of the second counting disc (516) at the preset angle, a counting button (513) is pressed for 12 times, the first counting disc (515) toggles a complete circle, and the second counting disc (516) turns a part by a preset angle and turns a part clockwise from the last letter to the next letter;

the piston assembly (2) comprises a piston rod (22) with a push button (21) fixedly connected to the top, an upper boss (23) is arranged at the lower part of the piston rod (22), a lower boss (24) is fixedly connected in a shell below the upper boss (23), the bottom end of the piston rod (22) penetrates through the lower boss (24) to be connected with the push device (3), and a return spring (25) is arranged on the piston rod (22) between the upper boss and the lower boss;

the shell (1) comprises an upper shell (11) and a lower shell (12), the upper shell (11) is in plug connection with the lower shell (12), an intermediate platform (13) is arranged in the lower shell (12), a sample tube socket (14) is arranged on the intermediate platform (13), a plug hole which is adaptive to the shape of the bottom of a sample tube (41) is arranged in the middle of the sample tube socket (14), and the bottom of the sample tube (41) is fixedly plugged in the plug hole;

the touch pressing assembly (52) comprises a sleeve (521) and a touch pressing rod (522), the sleeve (521) is fixedly connected to the shell (1), the touch pressing rod (522) is movably sleeved in the sleeve (521), the diameter of the touch pressing rod (522) is matched with the inner diameter of the sleeve (521), and a return spring (523) is arranged between the bottom of a top disc of the touch pressing rod and the top surface of the sleeve (521).

2. A pipetting device as recited in claim 1, wherein the top of the liquid pump (42) is fixedly connected with the push rod (31) in a plug-in connection.

3. A pipetting device for continuous sample application as recited in claim 1 wherein a central hole is provided in the middle of the intermediate platform (13), a guide tube (15) is provided from the central hole to the bottom opening of the housing (1), and the liquid delivery tube (43) extends out of the housing (1) after passing through the guide tube (15).

4. A pipetting device as recited in claim 1, wherein a pressing plate (524) having a diameter larger than the diameter of the counting button (513) is provided at the bottom of the pressing bar (522).