CN112370390B - Fermentation liquor for resisting aging, removing wrinkles and repairing skin barrier and preparation method thereof - Google Patents
Fermentation liquor for resisting aging, removing wrinkles and repairing skin barrier and preparation method thereof Download PDFInfo
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- CN112370390B CN112370390B CN202011367324.0A CN202011367324A CN112370390B CN 112370390 B CN112370390 B CN 112370390B CN 202011367324 A CN202011367324 A CN 202011367324A CN 112370390 B CN112370390 B CN 112370390B
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- China
- Prior art keywords
- fermentation
- salicornia
- streptococcus thermophilus
- polygonum tinctorium
- salicornia europaea
- Prior art date
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Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
- A61K8/645—Proteins of vegetable origin; Derivatives or degradation products thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
-
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Abstract
The invention relates to a fermentation liquor for resisting aging, removing wrinkles and repairing skin barriers and a preparation method thereof. The preparation method of the fermentation liquor comprises the step of fermenting salicornia europaea and polygonum tinctorium by using Streptococcus thermophilus grx90 with the preservation number of CGMCC No. 3622. The inventor adopts streptococcus thermophilus to carry out combined fermentation on salicornia europaea and polygonum glaucescens, and macromolecular substances in the salicornia europaea can be decomposed into micromolecular substances, so that the skin absorption is easy; the fermentation can also reduce the salt content in salicornia europaea and reduce the stimulation to the skin; the streptococcus thermophilus fermentation can also decompose the vegetable protein component in the Polygonum tinctorium blue into small molecular peptide, which is more beneficial to nourishing the skin.
Description
Technical Field
The invention relates to a fermentation liquor for resisting aging, removing wrinkles and repairing skin barriers and a preparation method thereof.
Background
Salicornia europaea L is an annual herb of the genus Salicornia of the family Chenopodiaceae. It is rich in protein, vitamins, amino acid minerals and various active substances, can promote the skin to recover good barrier function, and provides excellent moistening effect; can also promote the synthesis of natural skin moisturizing factor NMF, promote the skin to be smooth and soft, and can be used in skin care products. Chinese patent CN 104274355A discloses that the fermentation liquor containing the compound extract of grass green salicornia and reed rhizome can prevent skin aging, and Chinese patent CN 103767972A discloses a moisturizing and water locking composition containing grass green salicornia, which has the effects of deeply moisturizing, durably locking water and the like.
Polygonum tinctorium (Polygonum tinctorium) is an annual herb plant of Polygonaceae. The Polygonum tinctorium contains indigo, tryptanthrin, indirubin, indigo naturalis ketone, beta-sitosterol, kaempferol, flavonoids, indigoside and other substances, has the effects of resisting virus, bacteria, inflammation, oxidation and the like, and is widely used in the fields of medicines and dyeing. Chinese patent CN 103599269a discloses a Chinese medicinal composition containing Polygonum tinctorium with heat clearing and anti-inflammatory effects. Chinese patent CN107253249A discloses a method for dyeing native indigo pigment wicker, which is to extract indigo pigment from Polygonum blue, wherein the application of Polygonum blue is mainly directed to the effective components of indigo, tryptanthrin, beta-sitosterol and the like, and is mainly applied to the fields of medicines and dyeing.
At present, in the field of cosmetics, salicornia europaea is mostly extracted by solvents such as water, alcohol and the like to obtain the salicornia europaea extract, so that the nutrient substances and functional components in the salicornia europaea cannot be fully utilized. For example, proteins of macromolecules contained in salicornia europaea are not easily absorbed by the skin. In addition, the salicornia europaea has high salt content, can cause irritation to the skin and cause dryness, and is more likely to cause discomfort particularly for people with sensitive skin. In addition, the nutrient components such as vegetable protein in the Polygonum tinctorium blue are not fully developed and utilized.
Disclosure of Invention
The inventor researches and discovers that the streptococcus thermophilus is adopted to carry out combined fermentation on the salicornia europaea and the polygonum glaucum, so that macromolecular substances in the salicornia europaea can be decomposed into micromolecular substances, and the skin absorption is easy; the fermentation can also reduce the salt content in salicornia europaea and reduce the stimulation to the skin; the streptococcus thermophilus fermentation can also decompose the vegetable protein component in the Polygonum tinctorium blue into small molecular peptide, which is more beneficial to nourishing the skin.
The Streptococcus thermophilus used by the invention is Streptococcus thermophilus (Streptococcus thermophilus) grx90, the preservation number is CGMCC No. 3622, and the Streptococcus thermophilus is preserved in the 'common microorganism center of China Committee for culture Collection of microorganisms' 2 months and 1 day 2010; and has been disclosed in chinese patent application CN 101906391A.
Specifically, the invention provides a preparation method of fermentation liquor, which comprises the step of fermenting salicornia europaea and polygonum glaucum by using Streptococcus thermophilus grx90 with the preservation number of CGMCC No. 3622.
According to the embodiment of the invention, the preparation method of the fermentation liquor further comprises the step of taking supernatant after the fermentation is finished, namely the fermentation liquor.
According to an embodiment of the present invention, the supernatant may be obtained by centrifugation or natural sedimentation.
According to the embodiment of the invention, in the preparation method of the fermentation liquor, the weight ratio of the salicornia europaea to the polygonum tinctorium is 2-12:2-12, preferably 3:7-7:3, and more preferably 5: 5. Specifically, the weight ratio of salicornia europaea to polygonum tinctorium can be 2:12, 2:10, 5:5, 3:7, 7:3, 10:2 or 12: 2.
Researches find that the salicornia europaea and the polygonum glaucescens in the weight ratio range are more favorable for fermentation to generate salicornia europaea active small molecular peptides and polygonum glaucescens active small molecular peptides, and can also promote the salicornia europaea active small molecular peptides and the polygonum glaucescens active small molecular peptides to play the roles of resisting aging, removing wrinkles and repairing skin barriers.
According to an embodiment of the invention, the salicornia europaea is selected from turquoise salicornia.
According to an embodiment of the present invention, the method for preparing the fermentation broth comprises inoculating the activated streptococcus thermophilus into a medium containing salicornia herbacea and Polygonum tinctorium blue for fermentation; and taking the supernatant after the fermentation is finished, namely obtaining the fermentation liquor.
Specifically, the Streptococcus thermophilus can be cultured by a method conventional in the art, for example, the method disclosed in CN 101906391A. For example, the culture can be performed specifically using MRS medium.
According to the embodiment of the invention, the culture medium containing salicornia europaea and Polygonum tinctorium contains 2-12 wt% of salicornia europaea and 2-12 wt% of Polygonum tinctorium.
According to the embodiment of the invention, the culture medium containing salicornia europaea and polygonum glaucoides also contains a carbon source, a nitrogen source and inorganic salts.
Specifically, the carbon source is preferably one or more selected from glucose, ribose, lactose, galactose, fructose, sucrose, maltose, mannose, trehalose, mannitol, and sorbitol. The nitrogen source is preferably one or more selected from peptone, beef extract powder and yeast extract powder.
According to the embodiment of the invention, in the culture medium containing salicornia europaea and polygonum tinctorium, the preferred carbon source is glucose, and the preferred nitrogen source is peptone, beef extract powder and yeast extract powder, so that streptococcus thermophilus fermentation is facilitated.
According to the embodiment of the invention, the culture medium containing salicornia europaea and polygonum glaucoides comprises the following components in percentage by mass: 2-10% of salicornia europaea, 2-10% of Polygonum tinctorium, 5-30% of glucose, 5-15% of peptone, 5-15% of beef extract powder, 2-8% of yeast extract powder, 0.1-0.5% of dipotassium phosphate, 0.1-0.5% of magnesium sulfate and 0.1-0.3% of manganese sulfate.
According to an embodiment of the invention, the medium containing salicornia europaea and polygonum tinctorium further comprises water, for example, to make up to 100% with water.
According to an embodiment of the invention, the temperature of the fermentation is 35-50 ℃, such as 36-42 ℃.
According to embodiments of the invention, the fermentation process may typically be a stationary culture, for example, typically for 12-36 hours.
The specific fermentation temperature and time are based on the fact that the proteins or peptides in the salicornia europaea and the polygonum tinctorium blue can be substantially and fully hydrolyzed, or the fact that enough active small molecular peptides are generated is taken as the basis.
After the fermentation is finished, the supernatant can be taken by centrifugation, such as 6000g centrifugation for 10 minutes. The obtained supernatant contains a large amount of salicornia europaea active small molecular peptides and Polygonum tinctorium active small molecular peptides.
The fermentation liquor contains salicornia europaea active small molecular peptide, polygonum glaucoides active small molecular peptide and a fermentation product of Streptococcus thermophilus.
The invention also comprises the fermentation liquor prepared by the method. Researches show that the fermentation liquor utilizes microorganisms to ferment salicornia europaea and Polygonum tinctorium blue, active ingredients in the salicornia europaea and Polygonum tinctorium blue are released through fermentation of streptococcus thermophilus, macromolecular active ingredients which are difficult to absorb and utilize in the salicornia europaea and Polygonum tinctorium blue are further degraded, and the effects of aging resistance, wrinkle removal and skin barrier repair of the fermentation liquor are improved. Therefore, the fermentation liquor prepared by the method has excellent functions of resisting aging, removing wrinkles and repairing skin barriers.
The supernatant may be further dried to form a powder using methods conventional in the art. Generally, the structure and function of the active small molecule peptide obtained by fermentation are not damaged, such as freeze drying or spray drying.
The invention also comprises the application of the fermentation liquor prepared by the method in preparing cosmetics, medicines or foods for resisting aging and/or removing wrinkles and/or repairing skin barriers.
The invention also provides a cosmetic, a medicine or a food, which contains the fermentation liquor as a main active ingredient or a unique active ingredient.
The composition and preparation of the above cosmetics, drugs or foods can be referred to the prior art.
The cosmetic may be cream, lotion, paste, ointment, pack, gel or lotion. Can be prepared into skin care water, essence, facial mask, stock solution, ampoule, lotion, cream, eye cream, etc. by mixing with known matrix, adjuvant, carrier, additive, etc. according to conventional method.
Based on the research, the invention also provides the application of Streptococcus thermophilus grx90 with the preservation number of CGMCC No. 3622 in preparing the fermentation liquor by taking salicornia europaea and polygonum blue as raw materials.
Streptococcus thermophilus is an important industrial lactic acid bacterium, is recognized as a safe food-grade microorganism, can metabolize saccharides, protein substances and synthetic amino acid substances, and can produce metabolites such as short-chain fatty acids, extracellular polysaccharides and peptides in the fermentation process. According to the invention, the salicornia europaea and the polygonum tinctorium are fermented by the streptococcus thermophilus, so that plant protein nutrient substances in the salicornia europaea and the polygonum tinctorium can be fully utilized, and meanwhile, the abundant nutrient components in the salicornia europaea and the polygonum tinctorium can promote the fermentation of the streptococcus thermophilus to generate more active components such as extracellular polysaccharides and peptides. The invention can decompose macromolecular protein in salicornia europaea into micromolecular peptide easy to be absorbed by skin, simultaneously reduce salt content in the extract, reduce stimulation to the skin, decompose plant protein in Polygonum tinctorium into micromolecular peptide, and nourish the skin. The fermentation liquor has the effects of resisting aging, removing wrinkles, repairing skin barriers and the like, and can be used for preparing cosmetics, medicines or foods.
Detailed Description
Streptococcus thermophilus (Streptococcus thermophilus) grx90 with preservation number CGMCC No:3622 has been deposited in China general microbiological culture Collection center (China Committee for culture Collection of microorganisms) at 2.1.2010.
The activated culture method of Streptococcus thermophilus (the preservation number of which is CGMCC number 3622) comprises the following steps: adding Streptococcus thermophilus (CGMCC No. 3622) into MRS culture medium, standing at 42 deg.C for activation culturing for 16 hr, centrifuging at 6000g for 3 min to collect thallus, adding sterile water to wash thallus for 2 times, and adding PBS protectant (0.8% NaCl, 0.02% KH) with equal volume2PO4、0.115％Na2HPO41% tryptone and 0.1% sodium glutamate) as a strain, and inoculating the strain into a culture medium containing turfgrass salicornia and polygonum blue for fermentation.
The Streptococcus thermophilus (Streptococcus thermophilus) with the preservation number of CGMCC No.1.3996 is purchased from China general microbiological culture Collection center.
The method for activating Streptococcus thermophilus (Streptococcus thermophilus) CGMCC NO.1.3996 used below is substantially the same as that for Streptococcus thermophilus (Streptococcus thermophilus) grx90 (preservation number CGMCC No:3622) described above.
The Lactobacillus fermentum (Lactobacillus fermentum) with the preservation number of CGMCC number 1.15608 is purchased from China general microbiological culture Collection center. The activation method comprises adding Streptococcus thermophilus into MRS culture medium, standing at 36 deg.C for 48 hr, centrifuging (6000g, 3 min) to collect thallus, adding sterile water to wash thallus (2 times), and adding equal volume of activated Streptococcus thermophilus PBS protectant (0.8% NaCl, 0.02% KH)2PO4、0.115％Na2HPO41% tryptone and 0.1% sodium glutamate).
Example 1
Activated Streptococcus thermophilus (Streptococcus thermophilus) grx90 (CGMCC No:3622) is inoculated into culture medium according to the proportion of 2% and is statically cultured for 24 hours at 42 ℃. After the fermentation is finished, centrifuging (6000g for 10 minutes) and taking supernatant fluid to obtain fermentation liquor.
Wherein the composition of the medium is: 5% of turfgrass, 5% of Polygonum tinctorium, 10% of glucose, 8% of peptone, 5% of beef extract powder, 3% of yeast extract powder, 0.1% of dipotassium hydrogen phosphate, 0.2% of magnesium sulfate, 0.1% of manganese sulfate and the balance of water.
Example 2
Activated cultured Streptococcus thermophilus (Streptococcus thermophilus) grx90 (preservation number CGMCC No:3622) is inoculated into a culture medium according to the proportion of 2 percent and is statically cultured for 24 hours at 36 ℃. After the fermentation is finished, centrifuging (6000g for 10 minutes) and taking supernatant fluid to obtain fermentation liquor.
The medium used in this example was the same as in example 1.
Example 3
Activated cultured Streptococcus thermophilus (Streptococcus thermophilus) grx90 (preservation number CGMCC No:3622) is inoculated into culture medium according to the proportion of 2% and is statically cultured for 24 hours at 42 ℃. After the fermentation is finished, centrifuging (6000g for 10 minutes) and taking supernatant fluid to obtain fermentation liquor.
Wherein the composition of the medium is: 3% of turfgrass salicornia, 7% of Polygonum tinctorium, 10% of glucose, 8% of peptone, 5% of beef extract powder, 3% of yeast extract powder, 0.1% of dipotassium hydrogen phosphate, 0.2% of magnesium sulfate, 0.1% of manganese sulfate and the balance of water.
Example 4
Activated cultured Streptococcus thermophilus (Streptococcus thermophilus) grx90 (preservation number CGMCC No:3622) is inoculated into culture medium according to the proportion of 2% and is statically cultured for 24 hours at 42 ℃. After the fermentation is finished, centrifuging (6000g for 10 minutes) and taking supernatant fluid to obtain fermentation liquor.
Wherein the composition of the medium is: 4% of turfgrass, 6% of Polygonum tinctorium, 10% of glucose, 8% of peptone, 5% of beef extract powder, 3% of yeast extract powder, 0.1% of dipotassium hydrogen phosphate, 0.2% of magnesium sulfate, 0.1% of manganese sulfate and the balance of water.
Comparative example 1
The fermentation broth, the preparation method and example 1 differ only in that the composition of the medium used is: 10% of Polygonum tinctorium, 10% of glucose, 8% of peptone, 5% of beef extract powder, 3% of yeast extract powder, 0.1% of dipotassium hydrogen phosphate, 0.2% of magnesium sulfate, 0.1% of manganese sulfate and the balance of water.
Comparative example 2
The fermentation liquor and the preparation method are different from the example 1 only in that the streptococcus thermophilus grx90(CGMCC No:3622) is replaced by the streptococcus thermophilus with the preservation number of CGMCC No. 1.3996.
Comparative example 3
The fermentation broth, preparation method differs from example 1 only in that the culture medium used: 10% of turfgrass, 10% of glucose, 8% of peptone, 5% of beef extract powder, 3% of yeast extract powder, 0.1% of dipotassium hydrogen phosphate, 0.2% of magnesium sulfate, 0.1% of manganese sulfate and the balance of water.
Comparative example 4
The preparation method of the salicornia europaea extract comprises the following steps: washing 5g of turfgrass salicornia with water, adding 10 times of water by weight, grinding for 2 minutes to obtain homogenate, filtering with 100-mesh filter cloth to obtain filtrate, standing the filtrate overnight, taking supernatant, adding purified water to adjust the volume to 100mL, and obtaining salicornia extract.
Comparative example 5
The comparative example provides a streptococcus thermophilus fermentation broth, and the preparation method comprises the following steps: activated cultured Streptococcus thermophilus (Streptococcus thermophilus) grx90 was inoculated into MRS medium for fermentation at an inoculum size of 2%. The specific fermentation condition is static culture at 42 ℃ for 24 hours in a culture medium. And after the fermentation is finished, 6000g of the fermentation broth is centrifuged for 10 minutes, and supernate is taken to obtain the streptococcus thermophilus fermentation broth.
Comparative example 6
The fermentation broth, the preparation method and example 1 differ only in that S.thermophilus grx90(CGMCC No:3622) is replaced by Lactobacillus fermentum with the preservation number CGMCC No. 1.15608.
Experimental example 1
Taking 100ml of the fermentation liquid obtained in the examples 1-4 and the extract liquid obtained in the comparative examples 1-3 and the extract liquid obtained in the comparative example 4 respectively, filtering by using a filter membrane with the molecular weight cutoff of 1000, and detecting the content of small molecular peptides in the filtrate by using a Coomassie brilliant blue kit. The results are shown in Table 1.
TABLE 1 Small molecule peptide content
Note: the "small molecule peptide content" in Table 1 means the small molecule peptide content (mg) in 100ml of the fermentation solutions of examples 1 to 4 and comparative examples 1 to 3 and the extract solution of Salicornia Herbacea of comparative example 4.
Experimental results show that the content of the small molecular peptide in the fermentation liquor of the examples 1-4 is obviously higher than that of the comparative examples 1-4, and the streptococcus thermophilus can decompose macromolecular proteins in the turfgrass salicornia and the polygonum blue into the small molecular peptide less than 1000 daltons, so that the streptococcus thermophilus is beneficial to skin absorption and releases active ingredients of the turfgrass.
Experimental example 2
Taking 100ml of each of the fermentation liquid of examples 1-2, the fermentation liquid of comparative examples 2-3 and the salicornia extract of comparative example 4, and detecting the content of sodium chloride in the fermentation liquid by a silver nitrate titration method, wherein the results are shown in Table 2
TABLE 2 sodium chloride content
| Experimental example 1 | Example 2 | Comparative example 2 | Comparative example 3 | Comparative example 4 | |
| Sodium chloride content | 60mg | 71mg | 92mg | 83mg | 150mg |
Note: in Table 2, "sodium chloride content" means the sodium chloride content (mg) in 100ml of the fermentation liquids of examples 1 to 2 and comparative examples 2 to 3 and the extract liquid of Salicornia Herbacea of comparative example 4.
Experimental results show that the content of sodium chloride in the fermentation liquid of the example 1 and the fermentation liquid of the example 2 is obviously lower than that of the comparative example 2 to 4, and the streptococcus thermophilus can effectively reduce the salt content in the grass green salicornia, reduce the irritation to the skin and better protect the skin.
Experimental example 3 evaluation test of antioxidant function of fermentation broth
The following experiments were carried out by diluting 100 times each of the fermentation liquids of examples 1 to 4, comparative examples 1 to 3,5 and 6 and the extract of Salicornia herbacea of comparative example 4 with purified water.
1. Evaluation of superoxide anion radical scavenging ability
4.5mL of 0.05mol/L Tris-HCl buffer solution with pH 8.2 was preheated in a water bath at 25 ℃ for 20 min. Then adding 1mL of sample and 0.4mL of 25mmol/L pyrogallol solution, mixing uniformly, reacting in a water bath at 25 ℃ for 5min, and adding 1.0mL of 8mol/L HCl to terminate the reaction. Absorbance values were measured at 299nm with Tris-HCl buffer as a reference. The blank used 1mL of sample solvent in place of the sample (i.e., 25mmol/L pyrogallol solution). The clearance (D) was calculated as follows.
Superoxide anion radical scavenging rate (%) [ 1- (A2/A1) ] x 100%
Wherein, A1 is the absorbance value of the blank control; a2 is the absorbance value of the sample.
2. Evaluation of hydroxyl radical scavenging ability
Sequentially adding 2mmol/L FeSO into a 25mL colorimetric tube4 3mL，1mmol/L H2O23mL, shaking up, then adding 3mL of salicylic acid with the concentration of 6mmol/L, shaking up, heating in a water bath at 37 ℃ for 15min, taking out, and measuring the absorbance; adding the solutions to be tested with certain concentrations, shaking, heating in water bath for 15min, and taking out to test their absorbance. The clearance of hydroxyl radical (. OH) by the sample was calculated by the following formula:
hydroxyl radical clearance (%) ([ A1-A2- (A1-A3) ]/A1 × 100%,
wherein A1 is the absorbance value of the reaction system before adding medicine; a2 is the absorbance value of the system after the OH is removed from the sample; a3 is the absorbance value of the system after blank control removal of. OH.
The results of the evaluation of antioxidant function of the above samples are shown in Table 3.
TABLE 3 evaluation of antioxidant function
The results in Table 3 show that the fermentation liquors of examples 1-4 have significantly higher radical scavenging rate than comparative examples 1-6, which indicates that the fermentation liquor of the present invention has excellent antioxidant properties, wherein the antioxidant properties of example 1 are optimal, and can increase skin antioxidant ability and delay skin aging.
Experimental example 4 analysis of skin nourishing and moisturizing effects
The skin nourishing and moisturizing effects of the fermentation liquids of examples 1-4, comparative examples 1-3,5 and 6 and the extract liquid of the salicornia europaea of comparative example 4 are evaluated by the following specific method:
Selecting 100 volunteers, wherein the age is 25-60 years old, 38 men and 62 women; groups were randomized into 10 groups of 10 people each. 5X 5CM were selected at the skin for the experiment, and the above samples were applied to the skin using a German CK skin moisture tester Corneometer CM 825. The test skin moisture content of each subject was measured under conditions of constant temperature and constant humidity (21 ℃ and 40% humidity) using a skin moisture tester before the start of application, the initial value after application (T0) and the change in skin moisture content of 30min (T1) and 8h (T2) were measured, and the test results are shown in table 4.
TABLE 4 skin moisture content measurement
| T0-initial value (%) | T1-30min(％) | T2-8h(％) | |
| Example 1 | 32.6 | 52.3 | 50.6 |
| Example 2 | 33.6 | 47.6 | 46.3 |
| Example 3 | 32.9 | 46.3 | 45.2 |
| Example 4 | 33.5 | 45.6 | 43.9 |
| Comparative example 1 | 34.1 | 39.6 | 38.2 |
| Comparative example 2 | 33.3 | 37.9 | 36.7 |
| Comparative example 3 | 32.6 | 38.2 | 37.5 |
| Comparative example 4 | 33.2 | 36.2 | 35.4 |
| Comparative example 5 | 33.8 | 35.9 | 35.6 |
| Comparative example 6 | 33.2 | 34.3 | 34.0 |
As can be seen from the test results in Table 4, the skin water content 30min after use of the fermentation liquid in examples 1-4 is obviously improved compared with that in comparative examples 1-6, wherein the best effect is shown in example 1, which shows that the fermentation liquid of the present invention can nourish and moisturize the skin, provide sufficient skin moisture, reduce wrinkles, repair skin barriers and delay skin aging.
Although the invention has been described in detail hereinabove with respect to a general description and specific embodiments thereof, it will be apparent to those skilled in the art that modifications or improvements may be made thereto based on the invention. Accordingly, such modifications and improvements are intended to be within the scope of the invention as claimed.
Claims (12)
1. A method for preparing fermentation broth comprises fermenting Salicornia Herbacea and Polygonum tinctorium blue with Streptococcus thermophilus grx90 with preservation number of CGMCC No. 3622.
2. The process for preparing a fermentation broth according to claim 1, wherein the weight ratio of salicornia europaea to polygonum tinctorium is 2-12: 2-12.
3. The process for preparing a fermentation broth according to claim 1, wherein the weight ratio of salicornia europaea to polygonum tinctorium is 3:7-7: 3.
4. A process for producing a fermentation broth according to any one of claims 1 to 3, which comprises inoculating activated Streptococcus thermophilus into a medium containing Salicornia herbacea and Polygonum tinctorium for fermentation; and taking the supernatant after the fermentation is finished, namely obtaining the fermentation liquor.
5. The method for preparing fermentation broth according to claim 4, wherein the culture medium containing Salicornia europaea and Polygonum tinctorium contains 2 wt% -12 wt% Salicornia europaea and 2 wt% -12 wt% Polygonum tinctorium.
6. The method for preparing the fermentation liquor according to claim 4, wherein the culture medium containing salicornia europaea and Polygonum tinctorium comprises the following components in percentage by mass: 2-10% of salicornia europaea, 2-10% of Polygonum tinctorium, 5-30% of glucose, 5-15% of peptone, 5-15% of beef extract powder, 2-8% of yeast extract powder, 0.1-0.5% of dipotassium hydrogen phosphate, 0.1-0.5% of magnesium sulfate and 0.1-0.3% of manganese sulfate.
7. A process for the preparation of a fermentation broth according to any one of claims 1-3, 5, 6, wherein the fermentation temperature is 35-50 ℃.
8. The method for preparing a fermentation broth according to claim 7, wherein the fermentation temperature is 36-42 ℃.
9. A fermentation broth produced by the production method according to any one of claims 1 to 8.
10. Use of the fermentation broth according to claim 9 for the preparation of a cosmetic, pharmaceutical or food product for anti-ageing and/or wrinkle-reducing and/or skin barrier repair.
11. A cosmetic, pharmaceutical or food product comprising the fermentation broth of claim 9 as a main active ingredient or as the only active ingredient.
12. Application of Streptococcus thermophilus grx90 with preservation number of CGMCC No. 3622 in preparing fermentation liquid by using salicornia europaea and Polygonum tinctorium as raw materials.
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