CN114533633B - Phellinus baumii ferment and preparation method thereof - Google Patents

Phellinus baumii ferment and preparation method thereof Download PDF

Info

Publication number
CN114533633B
CN114533633B CN202210049427.5A CN202210049427A CN114533633B CN 114533633 B CN114533633 B CN 114533633B CN 202210049427 A CN202210049427 A CN 202210049427A CN 114533633 B CN114533633 B CN 114533633B
Authority
CN
China
Prior art keywords
fermentation
phellinus baumii
phellinus
potato
baumii
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN202210049427.5A
Other languages
Chinese (zh)
Other versions
CN114533633A (en
Inventor
阮华君
陶俊俊
唐长明
詹国灿
顾佳峰
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Zhejiang Changshengniao Health Technology Co ltd
Original Assignee
Zhejiang Changshengniao Health Technology Co ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Zhejiang Changshengniao Health Technology Co ltd filed Critical Zhejiang Changshengniao Health Technology Co ltd
Priority to CN202210049427.5A priority Critical patent/CN114533633B/en
Publication of CN114533633A publication Critical patent/CN114533633A/en
Application granted granted Critical
Publication of CN114533633B publication Critical patent/CN114533633B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/98—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin
    • A61K8/987—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin of species other than mammals or birds
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A61K8/9783—Angiosperms [Magnoliophyta]
    • A61K8/9789—Magnoliopsida [dicotyledons]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P29/00—Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
    • A61K2800/59—Mixtures
    • A61K2800/592—Mixtures of compounds complementing their respective functions
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
    • A61K2800/85—Products or compounds obtained by fermentation, e.g. yoghurt, beer, wine

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Veterinary Medicine (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Birds (AREA)
  • Epidemiology (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biotechnology (AREA)
  • Botany (AREA)
  • Zoology (AREA)
  • Mycology (AREA)
  • Pain & Pain Management (AREA)
  • Rheumatology (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Organic Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Dermatology (AREA)
  • Medicines Containing Plant Substances (AREA)

Abstract

The disclosure provides a preparation method of a Phellinus baumii ferment, comprising: step one, carrying out activation, purification and expansion culture treatment on Phellinus baumii to obtain seed liquid; step two, inoculating the seed solution to a fermentation substrate for fermentation culture treatment to obtain fermentation liquor; the fermentation substrate is potato homogenate, wherein pearl powder is added; and thirdly, separating and sterilizing the fermentation liquor to obtain supernatant. Also disclosed are Phellinus baumii fermented products prepared therefrom, including Phellinus baumii fermentation broths and dry powders thereof. The invention provides a preparation method of a Phellinus baumii fermentation product, which comprises the steps of inoculating Phellinus baumii into potato homogenate, fermenting with pearls in a synergistic way, so as to prepare a fermentation product with excellent anti-inflammatory performance and whitening performance, and adding the fermentation product into a cosmetic formula as an effective component to prepare cosmetics such as a mask, essence, sun cream, emulsion and the like.

Description

Phellinus baumii ferment and preparation method thereof
Technical Field
The present disclosure belongs to the technical field of biological fermentation, and in particular relates to a Phellinus baumii strain ferment and a preparation method thereof.
Background
Phellinus baumii (Phellinus baumii pilat.) commonly known as Phellinus linteus, phellinus syringae, etc., belongs to the genus Phellinus of the order Aphyllophorales, polyporaceae. The Bomu wood layer pore fungus belongs to wood rot fungi, has strong decay power and causes white decay of heartwood. Phellinus baumii belongs to a fungus for crude drugs for years, and is firstly collected in Li Zhen Ben Cao gang mu. Traditional Chinese medicine considers that the Phellinus baumii has warm and flat nature, bitter and pungent taste, and enters liver and bladder channels, and is used for treating metrorrhagia, blood stranguria, rectocele, diarrhea, leukorrhagia, amenorrhea, spleen deficiency diarrhea and the like. A plurality of experimental researches show that polysaccharide and flavonoid compounds in Phellinus baumii have important biological activities and have important effects in treating cancers, protecting livers, enhancing immunity and repairing nerves.
However, reports on application of Phellinus baumii to cosmetics are currently blank. The inventor finds that the ferment obtained by the synergistic fermentation of the Phellinus baumii and the pearl has excellent skin anti-inflammatory and repairing effects and whitening effects, and can be used as an effective component for cosmetics.
Disclosure of Invention
The following presents a simplified summary of the disclosure in order to provide a basic understanding of some aspects of the disclosure. It should be understood that this summary is not an exhaustive overview of the disclosure. It is not intended to identify key or critical elements of the disclosure or to delineate the scope of the disclosure. Its purpose is to present some concepts in a simplified form as a prelude to the more detailed description that is discussed later.
In order to solve the technical problems, the technical scheme provided by the present disclosure is as follows:
in a first aspect, the present disclosure provides a method for preparing a Phellinus baumii ferment, comprising:
step one, carrying out activation, purification and expansion culture treatment on Phellinus baumii to obtain seed liquid;
step two, inoculating the seed solution to a fermentation substrate for fermentation culture treatment to obtain fermentation liquor; the fermentation substrate is potato homogenate;
and thirdly, separating and sterilizing the fermentation liquor to obtain supernatant.
In the preparation method of the Phellinus baumii fermentation product, phellinus baumii is a commercial product purchased from China general microbiological culture collection center (CGMCC) 5.1265 and CGMCC5.2061.
In the preparation method of the Phellinus baumii fermentation product, the mycelium volume percentage of the seed liquid in the first step is more than 80%.
Further, in the preparation method of the fermentation product of Phellinus baumii, the inoculation ratio of Phellinus baumii in the second step, that is, the volume ratio of the seed solution to the fermentation substrate is 1% -10% (such as 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, etc.).
In the preparation method of the Phellinus baumii ferment, pearl powder is also added into the ferment substrate; the pearl powder is 1-10wt% (such as 2wt%, 3wt%, 4wt%, 5wt%, 6wt%, 7wt%, 8wt%, 9wt%, etc.) of the potato homogenate; more preferably 1-5wt% (e.g., 1.5wt%, 2wt%, 3wt%, 4wt%, 4.5wt%, etc.).
Further, in the preparation method of the Phellinus baumii fermentation product, the potato homogenate is prepared by homogenizing fresh potato slices and water by a homogenizer, and the potato homogenate is dissolved, wherein the content of the potato is 0.5-5wt% (such as 1wt%, 1.5wt%, 2wt%, 2.5wt%, 3wt%, 3.5wt%, 4wt%, 4.5wt%, etc.).
The fermentation substrate is subjected to a conventional sterilization treatment, such as high temperature sterilization at 115 ℃ for 20min, prior to inoculation.
Further, in the preparation method of the Phellinus baumii fermentation product, the potato homogenate further comprises 0.3wt% of monopotassium phosphate, 0.15wt% of magnesium sulfate, 0.05wt% of glucose and 0.2wt% of soybean peptide.
Further, in the preparation method of the Phellinus baumii fermentation product, the fermentation culture treatment temperature is 20-35 ℃ (such as 22 ℃,28 ℃, 30 ℃, 32 ℃, 34 ℃ and the like), and the fermentation culture treatment time is 48-96 hours (such as 52 hours, 60 hours, 72 hours, 84 hours, 88 hours, 92 hours and the like).
Further, the fermentation culture treatment is performed in a shaking table with a rotation speed of 100r/min-200r/min (such as 110r/min, 120r/min, 130r/min, 140r/min, 150r/min, 160r/min, 170r/min, 180r/min, 190r/min, 195r/min, etc.).
Further, in the preparation method of the Phellinus baumii fermentation product, the separation treatment adopts a centrifugal method; more preferably, the centrifugal speed is 4500r/min-5500r/min (such as 4600r/min, 4800r/min, 5000r/min, 5200r/min, 5400r/min, etc.), and the centrifugal time is 10min-20min (such as 12min, 15min, 18min, etc.).
In a second aspect, the present disclosure provides a Phellinus baumii fermentation broth made by the above-described method of making. The Phellinus linteus fermented product comprises Phellinus linteus fermentation liquid and dry powder thereof, wherein the dry powder can be prepared by freeze drying method or spray drying method.
In a third aspect, the present disclosure also provides a cosmetic comprising the above Phellinus baumii ferment. The cosmetic can be facial mask, essence, cream, lotion, etc.
Compared to the prior art, the benefits of the present disclosure include, but are not limited to:
1. the invention provides a preparation method of a Phellinus baumii ferment, which comprises the steps of inoculating Phellinus baumii into potato homogenate, fermenting with pearl in a synergistic way, so as to prepare a ferment with excellent anti-inflammatory performance and whitening performance, and adding the ferment into a cosmetic formula as an effective component to prepare cosmetics such as a mask, an essence, a sun cream, an emulsion and the like;
2. the Phellinus baumii fermentation product provided by the disclosure has the characteristics of strong efficacy, low cost, simplicity in operation, green safety and the like as an efficacy component of cosmetics, and has strong practicability and popularization value.
Drawings
Fig. 1 is a schematic diagram of the detection result of the anti-inflammatory effect of the protection treatment of the Phellinus linteus fermented product in example 2, and the index is: IL-1 beta;
FIG. 2 is a schematic diagram showing the detection results of the anti-inflammatory effect of the protection treatment of the extract of the pearl potato in example 2, which is an index: IL-1 beta;
fig. 3 is a schematic diagram of the detection results of the anti-inflammatory effect of the protective treatment of the Phellinus linteus ferments with different concentrations in example 2, and the indexes are as follows: IL-1 beta;
fig. 4 is a schematic diagram of the detection result of the anti-inflammatory effect of the rehabilitation treatment of the Phellinus linteus fermented product in example 2, and the indexes are as follows: IL-1 beta;
FIG. 5 is a graph showing the detection results of the anti-inflammatory effect of the repair treatment of the extract of the potato in example 2, the indexes: IL-1 beta;
fig. 6 is a schematic diagram of the detection results of the anti-inflammatory effect of the repair treatment of the Phellinus linteus ferments with different concentrations in example 2, and the indexes are as follows: IL-1 beta;
FIG. 7 is a schematic diagram showing the detection results of the anti-inflammatory effect of the repair treatment of the extract of the potato in example 2, the index: IL-8;
fig. 8 is a schematic diagram of detection results of antiinflammatory effects of repairing treatment of Phellinus linteus ferments with different concentrations in example 2, and indexes: IL-8.
Detailed Description
The following examples further illustrate the invention but are not to be construed as limiting the invention. Modifications and substitutions of the methods, steps or conditions of the present invention without departing from the spirit and nature of the invention are intended to be within the scope of the present invention.
Technical aspects of the present disclosure will be described hereinafter with reference to exemplary embodiments. The experimental methods used in the following examples are conventional methods unless otherwise specified. Materials, reagents and the like used in the examples described below are commercially available unless otherwise specified.
The Phellinus baumii used in the examples is a commercial product purchased from China general microbiological culture Collection center, and has a preservation number of: CGMCC5.2061.
Example 1 preparation of Pearl fermentate by Phellinus baumii fermentation experiments
(1) Activating strains: bacterial colonies are picked from the inclined plane of the preserved Bowm Phellinus CGMCC5.2061 (laboratory number 6984, bacterial strain is marked by laboratory number hereinafter), placed in a glucose potato agar culture medium for 7d culture and activation at 28 ℃, then the obtained single bacterial colonies are inoculated into 100mL glucose potato liquid culture medium for 7d culture at 28 ℃ and 180rpm, so that ganoderma lucidum bacterial liquid is obtained, and the volume ratio of mycelia accounts for 80% of the whole seed liquid.
(2) Preparing a fermentation substrate: according to the arrangement of Table 1, pearl powder with a certain mass ratio (1-10wt%, such as 1wt%, 5wt%, 10 wt%) is added into potato homogenate; the potato homogenate is prepared by cutting potato into pieces 20g, adding mineral water (containing 0.3% potassium dihydrogen phosphate, 0.15% magnesium sulfate, 0.05% glucose, and 0.2% soybean peptide) 1000ml, homogenizing in a homogenizer, dissolving, and packaging. The fermentation substrate is sterilized prior to inoculation.
(3) Inoculating and shaking: according to the inoculation amount of 5% by volume, 5mL of seed liquid is inoculated into a 250mL triangular flask filled with 100mL of liquid fermentation medium, and fermented in a shaking table at the rotation speed of 180r/min and the culture temperature of 28 ℃ for 48h.
(4) And (3) centrifuging: the fermentation broth was centrifuged at 4800r/min for 10min, the supernatant was taken, sterilized, and the obtained sample numbers were as shown in Table 1.
The example also provides a control group with a 2% potato homogenate of pure fermentation substrate, with 0.3% potassium dihydrogen phosphate, 0.15% magnesium sulfate, 0.05% glucose, 0.2% soy peptide added. See table 1 for details:
TABLE 1 fermentation regime and experimental group designation for example 1
Experiment group number Fermentation substrate Inoculation situation
TD-0 Simple fermentation of substrates Not inoculated with
TD-6984 Simple fermentation of substrates Inoculating 6984 strain
1％ZZ-TD-6984 Fermentation substrate containing 1% pearl powder Inoculating 6984 strain
5％ZZ-TD-6984 Fermentation substrate containing 5% pearl powder Inoculating 6984 strain
10％ZZ-TD-6984 Fermentation substrate containing 10% pearl powder Inoculating 6984 strain
1％ZZ-TD Fermentation substrate containing 1% pearl powder Not inoculated with
5％ZZ-TD Fermentation substrate containing 5% pearl powder Not inoculated with
10％ZZ-TD Fermentation substrate containing 10% pearl powder Not inoculated with
The main components of the product obtained in example 1, including protein, amino acid and calcium content, were tested and the results are shown in tables 2-4 below.
TABLE 2 amino acid content (umol/mL) of stock solution or fermentation stock solution samples
TABLE 3 protein content of stock solutions or fermentation stock solutions sample (ug/mL)
TABLE 4 calcium content of stock solutions or fermentation stock solutions
Experiment group number Calcium (mg/kg) Experiment group number Calcium (mg/kg)
TD-0 12.4 TD-6984 5.9
1％ZZ-TD 39.0 1％ZZ-TD-6984 57.2
5％ZZ-TD 33.1 5％ZZ-TD-6984 41.1
10％ZZ-TD 28.2 10％ZZ-TD-6984 22.5
Example 2 analysis of efficacy of Phellinus baumii fermentation broth
1. Toxicity and protective action of Phellinus baumii fermentation product on human skin fibroblast (HSF cell)
1. Establishment of oxidative stress model
HSF cells were 1X 10 per well 4 Inoculating into 96-well plate, culturing overnight in cell culture box, discarding culture medium, and adding 100 μl of different concentrations of H 2 O 2 (50～1000μmol·L -1 ) 5 replicates for each group and no H was added to the control group 2 O 2 . After 1, 2, 3, 4h of stimulation, the OD of each well was measured at 490nm using the MTT method. The HSF cell viability was obtained (see fig. 1), and hydrogen peroxide concentration at which the cell viability was 50% was selected as the modeling concentration. In this example, 100. Mu. Mol.L -1 H of (2) 2 O 2 HSF cells were treated for 2h modeling and cell viability was (49.74.+ -. 2.99)%.
2. Sample pair H 2 O 2 Protection and repair detection of induced oxidative stress model
HSF cells were 1X 10 per well 4 The samples were inoculated into 96-well plates and cultured overnight in a cell culture incubator, and a Control group (Control), a Model group (Model), and each sample group (stock solution or fermentation stock solution in example 1) were each set with 3 replicates.
The protection detection is thatAfter the sample acts on the cells, the cells are damaged again, secretion conditions of IL-1 beta and the like of the cells are observed, and whether the sample has a protective effect or not is judged. The protection processing group in this embodiment: adding sample, culturing for 24 hr, and adding H 2 O 2 Stimulation was performed for 2h.
The repair effect detection means that after cell injury, a sample is used for effect, and whether the sample has repair effect or not is evaluated by measuring the content of IL-1 beta and the like. In this embodiment, the repair treatment group: first add H 2 O 2 After 2h of stimulation, 100. Mu.L of sample was added to each well for 24h of incubation.
The test indexes are as follows: IL-1 beta, IL-8 (test methods refer to kit instructions). The results are shown in FIGS. 1-8.
Fig. 1 is a schematic diagram of the detection result of the anti-inflammatory effect of the protection treatment of the Phellinus baumii pearl ferment in example 2, and the index is: IL-1 beta. From the graph, the IL-1 beta of the model group is extremely higher than that of the blank control group, which indicates that the model is successfully established, 10% ZZ-TD has no significant difference compared with the model group, and after inoculation, 10% ZZ-TD-6984 is extremely lower than that of the model group, which indicates that fermentation broth after 6984 inoculation has the effect of reducing the secretion of IL-1 beta and has anti-inflammatory effect.
FIG. 2 is a schematic diagram showing the detection results of the anti-inflammatory effect of the protection treatment of the extract of the pearl potato in example 2, which is an index: IL-1 beta. From the graph, the IL-1β of the model group is extremely higher than that of the blank control group, which indicates that the model is successfully established, and the differences of the influence of the pearl potato extracts with different proportions on the IL-1β are larger: when the pearl proportion is 0, the effect of reducing IL-1 beta is not achieved; the pearl potato extract added with 1-5% of pearl powder has extremely remarkable effect of reducing IL-1 beta, and shows that the pearl potato extract has anti-inflammatory effect, and when the adding proportion of the pearl is increased to 10%, the pearl potato extract has no remarkable difference on the effect of IL-1 beta and does not have anti-inflammatory effect.
FIG. 3 is a schematic diagram showing the detection results of the anti-inflammatory effect of the protective treatment of Phellinus linteus pearl ferments with different concentrations in example 2, and the indexes are as follows: IL-1 beta. From the figure, the IL-1 beta of the model group is extremely higher than that of the blank control group, which indicates that the model establishment is successful, and the fermentation product obtained by inoculating the Phellinus baumii 6984 into the potato culture medium without pearl addition can not reduce the secretion of the IL-1 beta, and has no anti-inflammatory effect; however, when 10% pearls are added in proportion, the plating of Phellinus baumii 6984 can extremely significantly reduce IL-1 beta.
Fig. 4 is a schematic diagram of the detection result of the anti-inflammatory effect of the repair treatment of the Phellinus baumii pearl ferment in example 2, and the indexes are as follows: IL-1 beta. From the figure, the IL-1 beta of the model group is extremely higher than that of the blank control group, which indicates that the model is successfully established, and the effect of the potato extract with the same pearl addition proportion on the IL-1 beta before and after fermentation is compared, so that the obtained fermented product after the Bowm wood layer pore fungus 6984 is inoculated, and the effect of reducing the IL-1 beta is not obvious compared with the effect before fermentation.
FIG. 5 is a graph showing the detection results of the anti-inflammatory effect of the repair treatment of the extract of the potato in example 2, the indexes: IL-1 beta. From the figure, the IL-1 beta of the model group is extremely higher than that of the blank control, which indicates that the model is successfully established, the pearl potato extract with the pearl adding proportion of 5 percent has a repairing effect, and the pearl potato extract with the pearl adding amount of 1 percent and the pearl adding amount of 10 percent has no reducing effect on the IL-1 beta.
FIG. 6 is a schematic diagram showing the detection results of the anti-inflammatory effect of the repair treatment of the Phellinus linteus pearl ferments with different concentrations in example 2, and the indexes are as follows: IL-1 beta. From the graph, the IL-1 beta of the model group is extremely higher than that of the blank control group, which indicates that the model is successfully established, and the secretion of the IL-1 beta can not be reduced in the potato culture medium without pearl addition, both before and after fermentation, and the potato culture medium has no anti-inflammatory effect; the fermentation broth obtained after fermentation of the potato extract added with pearl also has no effect of reducing IL-1 beta.
FIG. 7 is a schematic diagram showing the detection results of the anti-inflammatory effect of the repair treatment of the extract of the potato in example 2, the index: IL-8. From the figure, the IL-8 of the model group is extremely higher than that of the blank control group, which indicates that the model establishment is successful, the pearl potato extract with the addition of 10% of pearls has the effect of reducing the IL-8, namely the repairing effect, but the potato extract with the addition of 1% and 5% of pearls has no anti-inflammatory effect.
FIG. 8 is a schematic diagram showing the detection results of the anti-inflammatory effect of the repair treatment of the Phellinus linteus pearl ferments with different concentrations in example 2, and the indexes are as follows: IL-8. From the figure, the model group IL-8 is extremely higher than that of the blank control group, which indicates that the model establishment is successful, the potato fermentation liquid without pearl addition, which is fermented by the Phellinus baumii 6984, can not reduce the secretion of IL-1 beta, and indicates that the potato fermentation liquid has no anti-inflammatory effect; the potato fermentation liquor with the pearl addition amount of 1-10% has the effect of reducing IL-8, which indicates that the potato fermentation liquor has the repairing effect.
2. Test for inhibiting tyrosinase activity by Phellinus baumii pearl fermentation product
1. Principle of experiment
The melanin is formed by gradually converting tyrosine in skin melanocyte tissue into eumelanin under the action of tyrosinase and other enzymes via dopa, dopaquinone, dopachrome, dihydroxyindole and other intermediates; furthermore, melanocyte tissue transfers melanin into keratin cells and falls off as the stratum corneum is periodically refreshed, and the difference in human skin color is mainly dependent on the content and distribution of eumelanin and pheomelanin, but when eumelanin grows excessively and is unevenly distributed, local skin hypermelanin or pigmentation is caused. There are three major enzymes involved in melanin synthesis: tyrosinase, dopachrome tautomerase and dihydroxyindole carboxylic acid oxidase. Tyrosinase is an oxidation-reduction enzyme, is a main speed-limiting enzyme for melanin synthesis, the activity of the tyrosinase is used for determining the quantity of melanin formation, and a plurality of whitening and freckle-removing products sold on the market at present are used for inhibiting the activity of tyrosinase to achieve the whitening effect, so that the inhibition effect on tyrosinase is a main index for evaluating whitening cosmetics. The L-tyrosinase and its substrate L-tyrosine can undergo catalytic reaction. When a reagent having an L-tyrosinase activity inhibiting effect is added to the experimental system, an inhibiting effect can be produced on the catalytic reaction, and the inhibition rate of the reagent on the L-tyrosinase activity can be evaluated by measuring absorbance at 475nm before and after the addition of the reagent.
2. Preparation of experiments
1) PBS phosphate buffer formulation (ph=6.8): 17.91g of disodium hydrogen phosphate dodecahydrate is taken and dissolved in distilled water to a constant volume of 500mL; dissolving 7.8g of sodium dihydrogen phosphate dihydrate in distilled water to a volume of 500mL; 92.6mL and 107.4mL of the two solutions were prepared as 200mL PBS phosphate buffer at pH 6.8.
2) 0.1mol/L hydrochloric acid solution: 0.862mL of HCl solution with 36-38% of HCl content is used, and distilled water is used for constant volume to 100mL.
3) 0.05% L-tyrosine solution: 0.05g g L-tyrosine was dissolved in 35ml of 0.1mol/L HCl solution, and 65ml of PBS phosphate buffer, pH6.8, was added thereto, 100ml in total.
4) Tyrosinase solution: the tyrosinase powder was diluted with PBS buffer to give a tyrosinase solution with an enzyme activity of 100U/mL.
3. Experimental procedure
1) After the C2 tube is uniformly mixed and shaken, the mixture is heated in a water bath for 10min in a water bath kettle at 37 ℃ and zeroed at the wavelength of 475 nm.
2) The C1 tube solution was prepared and shaken well, after 10min in a 37℃water bath, 1ml of tyrosinase was added, and the water bath was continued for 10min to determine the C1 absorbance.
3) The absorbance of T1 was measured by zeroing T2 in the same manner as in 1) and 2).
4) And calculating the activity inhibition rate T (%) of the stock solution or fermentation stock solution sample to tyrosinase.
TABLE 5 Experimental reagent proportioning table for tyrosinase inhibition rate
Numbering device L-tyrosine Sample of PBS Tyrosinase enzyme Total volume of
C1 2mL —— 4mL 1mL 7ml
C2 2mL —— 5ml —— 7ml
T1 2ml 2mL 2mL 1mL 7ml
T2 2ml 2ml 3ml —— 7ml
4. Data processing
The formula: t (%) = (C1-T1)/c1×100%. The results are shown in Table 6, and the data in the Table show that Phellinus baumii and Margarita are fermented, and the Phellinus baumii and Margarita have synergistic effect, and the whitening performance of the fermented product is remarkably improved.
TABLE 6 tyrosinase inhibition assay results
Finally, it is further noted that in this disclosure relational terms such as first and second, and the like are used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Moreover, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus. Without further limitation, an element defined by the phrase "comprising one … …" does not exclude the presence of other like elements in a process, method, article, or apparatus that comprises the element.
While the disclosure has been disclosed by the foregoing description of specific embodiments thereof, it will be understood that various modifications, improvements, or equivalents may be devised by those skilled in the art that will fall within the spirit and scope of the appended claims. Such modifications, improvements, or equivalents are intended to be included within the scope of this disclosure.

Claims (9)

1. The preparation method of the Phellinus baumii fermentation product is characterized by comprising the following steps:
step one, carrying out activation, purification and expansion culture treatment on Phellinus baumii to obtain seed liquid; the preservation number of the Phellinus baumii is CGMCC No.5.1265;
step two, inoculating the seed solution to a fermentation substrate for fermentation culture treatment to obtain fermentation liquor; the fermentation substrate is potato homogenate, wherein pearl powder is added, and the content of the pearl powder is 1-10wt% of the potato homogenate; the potato homogenate also comprises 0.3wt% of monopotassium phosphate, 0.15wt% of magnesium sulfate, 0.05wt% of glucose and 0.2wt% of soybean peptide; the temperature of the fermentation culture treatment is 20-35 ℃ and the time is 48-96 hours;
and thirdly, separating and sterilizing the fermentation liquor to obtain supernatant.
2. The method for producing a fermentation product of Phellinus baumii according to claim 1, wherein the seed liquid in the first step has a mycelium volume percentage of 80% or more.
3. The method for producing a fermentation product of Phellinus baumii according to claim 2, wherein the volume ratio of the seed liquid to the fermentation substrate in the second step is 1% -10%.
4. A process for the preparation of a fermentation product of Phellinus baumii according to any one of claims 1-3, characterized in that said pearl powder is present in an amount of 1-5% by weight of the potato homogenate.
5. A process for the preparation of a fermentation broth of Phellinus baumii according to any of claims 1-3, characterized in that said potato homogenate is obtained by homogenizing fresh potato slices with water by a homogenizer and dissolving, wherein the potato content is 0.5-5% by weight.
6. The method for producing a Phellinus baumii fermentation product according to claim 1, wherein said fermentation culture treatment in the second step is performed in a shaker at a rotation speed of 100r/min-200 r/min.
7. The method for producing a fermentation product of Phellinus baumii according to any one of claims 1-3 and 6, wherein in the third step, the separation treatment is performed by centrifugation.
8. The method for producing a fermentation product of Phellinus baumii according to claim 7, wherein the centrifugal speed is 4500r/min-5500r/min, and the centrifugal time is 10min-20min.
9. A fermentation broth of Phellinus baumii, characterized in that it comprises Phellinus baumii fermentation broth and dry powder thereof, and is prepared according to the preparation method of any one of claims 1-8.
CN202210049427.5A 2022-01-17 2022-01-17 Phellinus baumii ferment and preparation method thereof Active CN114533633B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202210049427.5A CN114533633B (en) 2022-01-17 2022-01-17 Phellinus baumii ferment and preparation method thereof

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202210049427.5A CN114533633B (en) 2022-01-17 2022-01-17 Phellinus baumii ferment and preparation method thereof

Publications (2)

Publication Number Publication Date
CN114533633A CN114533633A (en) 2022-05-27
CN114533633B true CN114533633B (en) 2024-02-27

Family

ID=81670728

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202210049427.5A Active CN114533633B (en) 2022-01-17 2022-01-17 Phellinus baumii ferment and preparation method thereof

Country Status (1)

Country Link
CN (1) CN114533633B (en)

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113208954A (en) * 2020-09-07 2021-08-06 北京工商大学 Anti-inflammatory and anti-aging ganoderma lucidum composite fermentation product and preparation method and application thereof

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113208954A (en) * 2020-09-07 2021-08-06 北京工商大学 Anti-inflammatory and anti-aging ganoderma lucidum composite fermentation product and preparation method and application thereof

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
可溶性珍珠粉和珍珠粉的抗炎作用比较;吴希美等;中国药理学会通讯;第第17卷卷(第第4期期);第50页 *
栎生桑黄和忍冬桑黄液体培养过程中发酵液抗氧化能力;王一菲等;研究论文;第第38卷卷(第第6期期);第938-950页 *
鲍氏层孔菌瓶栽生产技术;杜桂艳等;中国食用菌;第第24卷卷(第第4期期);第27-28页 *

Also Published As

Publication number Publication date
CN114533633A (en) 2022-05-27

Similar Documents

Publication Publication Date Title
CN113337545B (en) Schizophyllum commune fermentation product, preparation method thereof, skin care product and schizophyllum commune culture medium
JP2021019577A (en) Fermented birch sap and production method thereof
CN113367191B (en) Lactobacillus paracasei K56 for resisting oxidation and regulating blood pressure and application thereof
CN110680781B (en) White peony root fermented cosmetic and preparation method and application thereof
CN109694886B (en) Green tea fermentation filtrate and preparation method and application thereof
CN111053711A (en) Ganoderma lucidum probiotic compound fermentation product, preparation method and application thereof
JP2023097375A (en) Method for preparation of schizophyllan and ergothioneine by co-fermentation of two strains
CN109528568A (en) A kind of composition and preparation method thereof with anti-aging and white-skinned face function
CN113413351A (en) Fermentation liquor with whitening and anti-aging effects, fermented polypeptide, and preparation method and application thereof
CN115572738A (en) Dendrobium officinale fermentation liquor with anti-inflammatory, relieving and repairing effects as well as preparation method and application thereof
CN113842347B (en) Composition of black rose lucid ganoderma ferment and black rose enzymatic hydrolysate with anti-aging and whitening activities
KR100438009B1 (en) A cosmetic composition containing an extract of Grifola frondosa
CN106434755A (en) Inonotus obliquus submerged fermentation product and application
CN112691125B (en) Pharmaceutical composition for whitening or resisting aging, preparation method thereof and skin care product
CN113476388A (en) Bulbus Lilii fermented product for cosmetic and its preparation method
CN114533633B (en) Phellinus baumii ferment and preparation method thereof
CN111297741B (en) Preparation and application of pine pollen fermentation extracting solution
CN113350383A (en) Bifidobacterium lactis BL-99 capable of resisting oxidation and regulating blood pressure and application thereof
CN114699343B (en) Phellinus linteus fermentation product and preparation method thereof
CN111363687A (en) Lu's combined yeast strain, rose fermentation liquor containing same and application
CN113827523B (en) Rose composition and application thereof
KR100302505B1 (en) Fermented product using lactic acid bacteria and cosmetic composition
CN111544359B (en) Rice composite fermentation product with itching relieving effect and preparation method and application thereof
CN110638697B (en) Preparation and application of ganoderma lucidum lactobacillus fermentation extract
CN113648254A (en) Lupinus acutus and cherry fermentate for cosmetics and preparation method thereof

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant