CN1123289C - Slightly freezing method for storing silkworm ova - Google Patents
Slightly freezing method for storing silkworm ova Download PDFInfo
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- CN1123289C CN1123289C CN 00112577 CN00112577A CN1123289C CN 1123289 C CN1123289 C CN 1123289C CN 00112577 CN00112577 CN 00112577 CN 00112577 A CN00112577 A CN 00112577A CN 1123289 C CN1123289 C CN 1123289C
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Abstract
The present invention relates to a slightly freezing storage method of silkworm ova. The present invention uses a slightly freezing storage method which delays the time that the silkworm ova begins to be refrigerated under the temperature conditions of-0.5 to-5.5 DEG C. and a hatch controlling method which prolongs the impregnating time of the silkworm ova in hydrochloric acid to enhance the stimulus quantity of the hydrochloric acid. The slightly freezing storage method achieves the purposes that the silkworm ova can be supplied to silkworm breeding use in different periods from autumn in the current year to spring in next year, particularly the redundant silkworm ova which are refrigerated and soaked with hydrochloric acid in the current year can be effectively stored to be used in spring in the next year; the slightly freezing storage method solves the problem of the waste of the silkworm ova.
Description
Technical field
The present invention relates to a kind of slightly freezing method for storing of silkworm egg, particularly a kind of silkworm egg is year all spendable preservation and hatching control then and more.
Background technology
As everyone knows, silkworm is a kind of ovum diapause insect, and diapause betides embryo's initial stage of origination, when the environmental temperature of laying eggs back 25 ℃ is protected, begins diapause in back about 72 hours about laying eggs greatly.At present, the store method of silkworm egg all is at using then and getting over a year use and handle respectively both at home and abroad.The silkworm seed that uses adopts the acid-treatment after chilling method autumn, winter then, gives birth to the back 44～54 hours at silkworm seed, with 5 ℃ temperature refrigerations 40～70 days, uses the salt acid treatment then, and supply autumn, winter, cocoonery need be with planting.Owing to before silkworm seed enters diapause, use refrigeration to handle, stoped the further diapause of silkworm seed, use the salt acid treatment to impel silkworm seed to break diapause subsequently, begin activation.To a silkworm seed that uses year spring more is to adopt the method that begins to refrigerate winter, and the silkworm seed after will giving birth to spring is protected with natural temperature, bathes wash disinfection to late November～early December, is saved in the method for single entry or compound refrigeration then and uses 1 year spring.The method of simple refrigeration is to begin to finish the first tenday period of a month in late April, second to May to hasten the hatching of silkworms after the refrigeration with 2.5 ℃ of refrigeration silkworm eggs in late January to mid-February, and silkworm in spring is used in supply; The method of compound refrigeration is to begin temperature refrigeration silkworm seed with 0 ℃ or 2.5 ℃ in the mid-January of late December to the second year, at the beginning of 3 months, carry out the centre temperature-sensitive with 10～15 ℃ temperature, return when treating embryonic development and continue refrigeration under 2.5 ℃ the temperature to critical period, finish refrigeration the first tenday period of a month in late April to May, and the back supply silkworm in spring of hastening the hatching of silkworms is used.
Sericulture is owing to influenced by weather, market and production distribution etc., great changes have taken place with actual use meeting for silk cocoon production schedule sowing quantity, the silkworm egg supply will accomplish that the equilibrium of supply and demand has very big difficulty, and use autumn then with year uses the preservation technical system of silkworm seed different fully spring more, can not change mutually, therefore annual have a large amount of acid-treatment after chilling silkworm eggs to waste.Up to the present, yet there are no can autumn is unnecessary the acid-treatment after chilling silkworm seed effectively be saved in the report that uses in next year spring.
Summary of the invention
The objective of the invention is to overcome the above-mentioned autumn with the acid-treatment after chilling silkworm egg and more year silkwormrace for spring rearing can't adopt shortcoming with a kind of store method, provide a kind of can the supply to use the store method that can satisfy the silkworm seed that uses in year more again then, acid-treatment after chilling then can be made exhaustless silkworm seed effectively be saved in and use 1 year spring, solve the waste problem of silkworm egg.
The technical scheme that realizes the object of the invention is: silkworm egg is adopted little method of freezing preservation, it is characterized in that:
A. the silkworm egg after laying eggs was preserved in 23～25 ℃ temperature environment 10～30;
B. after preserving end silkworm egg is carried out the refrigeration first time in 2.5～5 ℃ temperature environment;
C. silkworm egg is stopped refrigeration for the first time at any time with the interior any time for the first time refrigerating 100, then through wash sterilization, little operations such as preservations, middle temperature-sensitive, refrigeration for the second time of freezing are handled, provide more year cocoonery to use and plant; Or after for the first time refrigerating 60～120, stop refrigeration for the first time, and silkworm egg is directly carried out pickling processings of hastening the hatching of silkworms, provide then autumn, the winter cocoonery need be with planting.
Above-described little temperature of freezing preservation is-0.5～-5.5 ℃; Temperature-sensitive, refrigeration for the second time are to begin silkworm egg is carried out centre temperature-sensitive under 10～12 ℃ of medium temperatures in early March, second in the middle of described, treat that the silkworm egg development of embryo arrives critical period, silkworm egg are turned back to again in 2.5～5 ℃ the temperature to refrigerate; Described the silkworm egg of using is then carried out the pickling processing of hastening the hatching of silkworms after finishing refrigeration for the first time, its pickling solution is industrial hydrochloric acid, the liquid temperature of hydrochloric acid is 47.5～48 ℃, and proportion is 1.093 under the purpose temperature, and silkworm seed dip time in hydrochloric acid is 8～30 minutes.
The present invention has increased the repellence of silkworm seed to low temperature owing to postponed the cold preservation time of acid-treatment after chilling; Add and adopted little method of freezing refrigeration, reduced the nutrient consumption of silkworm seed effectively, thereby silkworm seed can be saved in year use more; Also owing to adopted the dip time of prolongation silkworm seed in hydrochloric acid, strengthened the hydrochloric acid quantity of stimulus, thereby can be with the very inadequate silkworm seed activation of refrigeration activation, satisfy and are using in autumn and winter then, finally realized adopting a kind of store method, can supply the purpose of year different times sericulture use in spring in autumn to the second then, solve the waste problem of silkworm egg effectively.
Description of drawings
Fig. 1 is the flow chart of the slightly freezing method for storing embodiment of a kind of silkworm egg of the present invention.
Embodiment
Embodiment:
Referring to accompanying drawing 1, it is on June 15th, 1 that spring silkworm rearing season is made the silkworm egg time, and silkworm kind Su Zhen * spring scenery is collected 10 hours diapause gamogenetic egg of egg-laying time.Protection is 20 under the back 25 ℃ temperature of laying eggs; through 13 ℃ of medium temperatures after 12 hours; enter and carry out the refrigeration first time in 5 ℃ the temperature; use the silkworm egg part for the autumn; refrigerate 70 in the first time and finish refrigeration about (September 15 the same year); after the about 13 ℃ medium temperature in 6 hours, temperature; hasten the hatching of silkworms through the salt acid treatment; the method of salt acid treatment is: 48 ℃ of industrial salt acid solution temperature; hydrochloric acid proportion under the purpose temperature is 1.093 (concentration of hydrochloric acid 19%); silkworm egg flooded in hydrochloric acid 25 minutes, and silkworm egg is supplied to breed silkworms autumn then and used.
For the silkworm egg that uses in year more, proceed to the 90th day in refrigeration for the first time and finish refrigeration about (October 5 the same year), after the about 10 ℃ medium temperature in 5 hours, temperature, silkworm egg is bathed the arrangement that disappears, method is: wash and fall into water warm 20 ℃, sterilization Eusol available chlorine 0.15%, disinfecting time 10 minutes.Arrangement finishes the back and freezes preservation with-3 ℃ of temperature are little.
Freeze in the preservation process little, in about the March in 1 year 5, began silkworm egg to be carried out the centre temperature-sensitive, treat that the silkworm seed development of embryo is to critical period (Stage of criticaldevelopment, promptly third with 12 ℃ temperature
1～the third
1 +Period), silkworm egg is turned back to carry out the refrigeration second time in 5 ℃ the temperature.With planting, silkworm seed can finish refrigeration for the second time at any time as silkworm in spring, and the back supply sericulture in spring of hastening the hatching of silkworms is used.
Adopt the silkworm seed that technical solution of the present invention provided, autumn then silkworm and more year silkworm in spring use, can both reach practical requirement.Table 1 is for adopting acid-treatment after chilling method, simple refrigeration method and little test comparison results of freezing three kinds of method preservation quality of silkworm eggs such as preservation method respectively.Test silkworm kind is Su Zhen * spring scenery, and spring silkworm rearing season is made silkworm egg; D freezes the silkworm egg that method is preserved in the table for the present invention is little, and the autumn is used for pickling in September 24 and hastens the hatching of silkworms then, and year spring is used for hastening the hatching of silkworms on the April 20 in 1 year more; CK1 is normal acid-treatment after chilling silkworm egg, does not have the refrigeration silkworm egg of pickling then on September 24, bathe the arrangement that disappears after, hasten the hatching of silkworms the April 20 that always refrigerates 1 year with 2.5～5 ℃ temperature; CK2 is for adopting the silkworm egg of normal Nian Chunyong simple refrigeration method more protection, and on April 20th, 1 hastened the hatching of silkworms.Sampling and the method for testing used in the table 1 are: with 25 ℃ temperature, RH.75～85% environment hastens the hatching of silkworms, and the incubation rate day by day of the normal ovum of investigation 5 * 0.2g is added up the highest 2 days incubation rates.All are handled after February 10, Nian Chunyong simple refrigeration silkworm seed was put in storage more, the good ovum of enumeration 5 * 1.0g, regularly investigation bad egg incidence in freezer.5 * 100 investigation are used in every processing, and used mulberry leaf are handled, dredged hair silkworm number and dredge the hair rate in back 24 hours (28 ℃, the RH.75～85%) investigation of collecting ants with the leaf point-score between the processing.The every processing of number was with 5 * 50 investigation (28 ℃, RH.75～85%) when newly-hatched silkworm was gone on a hunger strike life, and certain hour is investigated dead silkworm number at interval, calculated half death time LT
50For reducing error, investigate with the artificial diet rearing method and play the silkworm percentage of missing larvae 3 ages.Every processing is with 5 * 50 investigation, and to bait once, 29 ℃, R.H.75～85% environmental protection to investigation survival silkworm number on the 9th, calculate percentage of missing larvae when collecting ants.
From table 1 comparing result as can be seen, use method provided by the invention, the incubation rate of silkworm egg behind acid-treatment after chilling then can reach more than 95%, and the physique of newly-hatched silkworm is suitable with normal acid-treatment after chilling egg-incubation newly-hatched silkworm; The incubation rate that more year refrigeration back silkworm in spring is used can reach more than 95%, and the physique of newly-hatched silkworm is with normal Nian Chunyong egg-incubation newly-hatched silkworm is suitable more.Use the inventive method is only used a kind of preservation mode, can supply simultaneously then and breeding silkworms with the purpose of planting year spring in autumn to the second, and fundamentally having solved then, acid-treatment after chilling makes exhaustless silkworm seed effectively be saved in the silkworm egg waste problem of use in 1 year spring.
Table 1
Autumn is used Nian Chunyong more to the project cold preserving method then
D 0.89
Dead ovum incidence CK1 10.85
CK2 0.35
D 98.0 98.1
Incubation rate (%) CK1 98.3 30.2 on the 2nd
CK2 92.2
D 116 114
LT
50(h) CK1 120 80
CK2 115
D 99.9 99.1
Dredge hair rate (%) CK1 99.8 89.8
CK2 99.6
D 7.6 8.1
Percentage of missing larvae (%) CK1 6.1 29.8
CK2 7.6
Claims (2)
1. the slightly freezing method for storing of a silkworm egg is characterized in that:
A. the silkworm egg after laying eggs was preserved in 23～25 ℃ temperature environment 10～30;
B. after preserving end silkworm egg is carried out the refrigeration first time in 2.5～5 ℃ temperature environment;
C. silkworm egg is stopped refrigeration for the first time at any time with the interior any time for the first time refrigerating 100, after washing sterilization, littlely under-0.5～-5.5 ℃ temperature environment freeze preservation, in early March, second begins silkworm egg is carried out the centre temperature-sensitive under 10～12 ℃ temperature, treat that the silkworm egg development of embryo is to critical period, again silkworm egg is turned back to refrigeration for the second time in 2.5～5 ℃ the temperature, provide more year cocoonery need with kind; Or after for the first time refrigerating 60～120, stop refrigeration, and silkworm egg is directly carried out pickling processings of hastening the hatching of silkworms, provide then autumn, the winter cocoonery need be with planting.
2. the slightly freezing method for storing of a kind of silkworm egg according to claim 1, the silkworm egg of using is then carried out the pickling processing of hastening the hatching of silkworms after finishing refrigeration for the first time, it is characterized in that: described pickling solution is industrial hydrochloric acid, the hydrochloric acid solution temperature is 47.5～48 ℃, proportion is 1.093 under the purpose temperature, and silkworm seed dip time in hydrochloric acid is 8～30 minutes.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 00112577 CN1123289C (en) | 2000-09-27 | 2000-09-27 | Slightly freezing method for storing silkworm ova |
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| CN 00112577 CN1123289C (en) | 2000-09-27 | 2000-09-27 | Slightly freezing method for storing silkworm ova |
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| CN1123289C true CN1123289C (en) | 2003-10-08 |
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Families Citing this family (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101361474B (en) * | 2008-09-19 | 2011-01-19 | 江苏科技大学 | Preparation method of silkworm commercial race transgene silkworm egg |
| CN102754624B (en) * | 2012-06-15 | 2013-10-23 | 如皋市蚕桑技术指导站 | Method for delaying hatching of F1 hybrids of silkworm eggs in hatching |
| CN103688910B (en) * | 2014-01-09 | 2015-04-15 | 浙江省农业科学院 | Method for inducing silkworm meiosis type parthenogernesis by liquid refrigerants |
| CN106508827B (en) * | 2016-11-08 | 2019-10-22 | 广东省农业科学院蚕业与农产品加工研究所 | A kind of method and application of the reduction without diapause polyvoltinism silkworm germ plasm resource child care algebra |
| CN107279075A (en) * | 2017-06-28 | 2017-10-24 | 合肥市聚丰制丝有限责任公司 | A kind of silkworm in spring egg hatching method |
| CN107372458A (en) * | 2017-07-15 | 2017-11-24 | 合肥市聚丰制丝有限责任公司 | A kind of silkworm eggs store method |
| CN107711730A (en) * | 2017-11-17 | 2018-02-23 | 华南农业大学 | The refrigeration hatching method of silkworm eggs |
| CN109769758A (en) * | 2019-03-25 | 2019-05-21 | 广东省农业科学院蚕业与农产品加工研究所 | A kind of heat treatment method blocking refrigeration silkworm egg pebrine disease germinal infection |
| CN112106735B (en) * | 2020-09-28 | 2023-04-28 | 贵州省烟草公司黔东南州公司 | Method for refrigerating diapause of myxoplasma |
| CN112106734B (en) * | 2020-09-28 | 2023-04-28 | 贵州省烟草公司黔东南州公司 | Refrigeration diapause method for armyworms |
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