CN107372458A - A kind of silkworm eggs store method - Google Patents
A kind of silkworm eggs store method Download PDFInfo
- Publication number
- CN107372458A CN107372458A CN201710577856.9A CN201710577856A CN107372458A CN 107372458 A CN107372458 A CN 107372458A CN 201710577856 A CN201710577856 A CN 201710577856A CN 107372458 A CN107372458 A CN 107372458A
- Authority
- CN
- China
- Prior art keywords
- parts
- silkworm
- paper
- eggs
- spawning
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
- 241000255789 Bombyx mori Species 0.000 title claims abstract description 74
- 238000000034 method Methods 0.000 title claims abstract description 31
- 235000013601 eggs Nutrition 0.000 title claims abstract description 30
- 238000012805 post-processing Methods 0.000 claims abstract description 5
- 230000000844 anti-bacterial effect Effects 0.000 claims description 18
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 10
- 238000012545 processing Methods 0.000 claims description 9
- 241001666377 Apera Species 0.000 claims description 5
- 244000025254 Cannabis sativa Species 0.000 claims description 5
- 235000001855 Portulaca oleracea Nutrition 0.000 claims description 5
- 238000001802 infusion Methods 0.000 claims description 5
- 238000002803 maceration Methods 0.000 claims description 5
- 239000011780 sodium chloride Substances 0.000 claims description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 5
- 241000132446 Inula Species 0.000 claims description 3
- 235000007516 Chrysanthemum Nutrition 0.000 claims description 2
- 244000189548 Chrysanthemum x morifolium Species 0.000 claims description 2
- 240000007594 Oryza sativa Species 0.000 claims description 2
- 235000007164 Oryza sativa Nutrition 0.000 claims description 2
- 241001494479 Pecora Species 0.000 claims description 2
- 235000009566 rice Nutrition 0.000 claims description 2
- 244000234609 Portulaca oleracea Species 0.000 claims 2
- 230000012447 hatching Effects 0.000 abstract description 11
- 230000000694 effects Effects 0.000 abstract description 5
- 238000002474 experimental method Methods 0.000 abstract description 3
- 230000000052 comparative effect Effects 0.000 description 6
- 102000002322 Egg Proteins Human genes 0.000 description 5
- 108010000912 Egg Proteins Proteins 0.000 description 5
- 210000004681 ovum Anatomy 0.000 description 5
- 241000219304 Portulacaceae Species 0.000 description 3
- 230000005058 diapause Effects 0.000 description 3
- 238000011534 incubation Methods 0.000 description 3
- 210000001161 mammalian embryo Anatomy 0.000 description 3
- 239000004480 active ingredient Substances 0.000 description 2
- 238000005138 cryopreservation Methods 0.000 description 2
- 238000004321 preservation Methods 0.000 description 2
- 241000238631 Hexapoda Species 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000002203 pretreatment Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
- A01K67/04—Silkworms
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0205—Chemical aspects
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0205—Chemical aspects
- A01N1/021—Preservation or perfusion media, liquids, solids or gases used in the preservation of cells, tissue, organs or bodily fluids
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0236—Mechanical aspects
- A01N1/0242—Apparatuses, i.e. devices used in the process of preservation of living parts, such as pumps, refrigeration devices or any other devices featuring moving parts and/or temperature controlling components
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Health & Medical Sciences (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Physics & Mathematics (AREA)
- Thermal Sciences (AREA)
- Mechanical Engineering (AREA)
- Animal Behavior & Ethology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Fodder In General (AREA)
Abstract
The present invention relates to a kind of silkworm eggs store method, following steps are specifically included：（1）The selection of spawning paper；（2）Spawning post processing；（3）The storage of silkworm seed.By experiment, using a kind of silkworm eggs store method provided by the invention, the hatching success of silkworm eggs is substantially increased；Using conventional cold preserving method, the hatching success of silkworm seed is only 42%, and uses the inventive method, and the hatching success of silkworm seed is promoted to more than 60%, significant effect.
Description
Technical field
The invention belongs to silkworm cultural technique field, and in particular to a kind of silkworm eggs store method.
Background technology
Silkworm is a kind of ovum diapause insect bred by ovum, and generally, the duration of diapause of silkworm seed is after spawning 7 days
Through summer, mid-autumn between late October, after silkworm seed enters the winter, contact a certain degree of low temperature, silkworm seed will gradual termination of diapause,
As active ovum, the silkworm seed of activation such as experiences certain temperature, and just development is so that hatching gradually for embryo of silkworms, therefore, more year silkworm egg
Need to refrigerate when entering the winter.
But prior art is used, silkworm egg incubation rate is not universal high, is generally all to change silkworm egg incubation pre-treatment side
Method improves the incubation rate of silkworm seed, and has not focused on the preservation of silkworm eggs.
The content of the invention
In order to solve the above problems, the invention provides a kind of silkworm eggs store method.
The present invention is achieved through the following technical solutions：
A kind of silkworm eggs store method, specifically includes following steps：
（1）The selection of spawning paper
Before silkworm moth lays eggs, it is placed on spawning bottom portion using Bactericidal paper and is laid eggs for silkworm moth, can be effective using the Bactericidal paper
The ovum for preventing from just having given birth to is stained with the germ on paper, ensures the health and safety of silkworm seed, while the active ingredient on Bactericidal paper can be helped
Silkworm seed outer wall quick-hardening is helped, silkworm seed is further improved and does not receive pollution in early stage；The Bactericidal paper, specifically by following methods system
:
A takes common print paper, and use quality fraction is 2% sodium chloride solution maceration, is then dried, and is flattened；
B according to the mass fraction, takes folium artemisiae argyi 15-20 parts, north wind grass 10-15 parts, purslane 10-15 parts, toad grass 8-12 parts, sheep ear
Infusion 15-20 minutes after chrysanthemum 2-5 parts, dried orange peel 2-5 parts, the mixing of water 500-600 parts, filter cleaner, obtain soak；
C fully soaks processing of step A good printing paper 12-15 hours using soak described in step B, dries, and flattens, i.e.,
；
（2）Spawning post processing
After silkworm moth spawning, the part that silkworm seed is carried on the Bactericidal paper is torn, shone using 40 watts of ultraviolet lamps at 2.5-3 rice
Processing 5-8 minutes are penetrated, is then placed under 10-15 DEG C of lucifuge, ventilated environment and stores 15-30 days；Silkworm seed is irradiated using ultraviolet
Processing, then in 10-15 DEG C of preservation, direct cryopreservation can be effectively avoided to cause silkworm seed can not adapt to environment drastic change and cause it
Hatchability is lost, and ultraviolet irradiation can stimulate the activity of embryo in silkworm seed, ensure surviving in its storage；
（3）The storage of silkworm seed
By step（3）The silkworm seed handled well refrigerates as in 0-4 DEG C of freezer.
Beneficial effects of the present invention：The present invention uses the Bactericidal paper, and the ovum that effectively can prevent from just having given birth to is stained with paper
Germ, ensure the health and safety of silkworm seed, while the active ingredient on Bactericidal paper can help silkworm seed outer wall quick-hardening, enter one
Step improves silkworm seed and does not receive pollution in early stage；After silkworm moth spawning, silkworm seed is used into ultraviolet treatment with irradiation, then in 10-15 DEG C of guarantor
Deposit, can effectively avoid direct cryopreservation from causing silkworm seed can not adapt to environment drastic change and cause it to lose hatchability, and it is ultraviolet
Line irradiates the activity that can stimulate embryo in silkworm seed, ensures surviving in its storage.It is provided by the invention by experiment, use
A kind of silkworm eggs store method, substantially increase the hatching success of silkworm eggs；Use conventional cold preserving method, the hatching of silkworm seed
Success rate is only 42%, and uses the inventive method, and the hatching success of silkworm seed is promoted to more than 60%, significant effect.
Embodiment
Embodiment 1
A kind of silkworm eggs store method, specifically includes following steps：
（1）The selection of spawning paper
Before silkworm moth lays eggs, it is placed on spawning bottom portion using Bactericidal paper and is laid eggs for silkworm moth；The Bactericidal paper, specifically by with lower section
Method is made:
A takes common print paper, and use quality fraction is 2% sodium chloride solution maceration, is then dried, and is flattened；
B according to the mass fraction, takes 15 parts of folium artemisiae argyi, 10 parts of north wind grass, 10 parts of purslane, 8 parts of toad grass, 2 parts of Sheepear Inula Herb, dried orange peel 2
Infusion 15 minutes, filter cleaner, obtains soak after 500 parts of part, water mixing；
C fully soaks the good printing paper of processing of step A 12 hours using soak described in step B, dries, and flattens, produces；
（2）Spawning post processing
After silkworm moth spawning, the part that silkworm seed is carried on the Bactericidal paper is torn, irradiated using 40 watts of ultraviolet lamps at 2.5 meters
Processing 5 minutes, is then placed under 10-15 DEG C of lucifuge, ventilated environment and stores 15 days；
（3）The storage of silkworm seed
By step（3）The silkworm seed handled well refrigerates as in 0-4 DEG C of freezer.
Embodiment 2
A kind of silkworm eggs store method, specifically includes following steps：
（1）The selection of spawning paper
Before silkworm moth lays eggs, it is placed on spawning bottom portion using Bactericidal paper and is laid eggs for silkworm moth；The Bactericidal paper, specifically by with lower section
Method is made:
A takes common print paper, and use quality fraction is 2% sodium chloride solution maceration, is then dried, and is flattened；
B according to the mass fraction, takes 20 parts of folium artemisiae argyi, 15 parts of north wind grass, 15 parts of purslane, 12 parts of toad grass, 5 parts of Sheepear Inula Herb, dried orange peel 5
Infusion 20 minutes, filter cleaner, obtains soak after 600 parts of part, water mixing；
C fully soaks the good printing paper of processing of step A 15 hours using soak described in step B, dries, and flattens, produces；
（2）Spawning post processing
After silkworm moth spawning, the part that silkworm seed is carried on the Bactericidal paper is torn, using 40 watts of ultraviolet lamps at 3 meters at irradiation
Reason 8 minutes, is then placed under 10-15 DEG C of lucifuge, ventilated environment and stores 30 days；
（3）The storage of silkworm seed
By step（3）The silkworm seed handled well refrigerates as in 0-4 DEG C of freezer.
Comparative example 1
This comparative example 1 compared with Example 1, without using step（1）The Bactericidal paper, method and step in addition are homogeneous
Together.
Comparative example 2
This comparative example 2 compared with Example 2, without using step（1）Methods described, method and step all same in addition.
Control group
Existing silkworm eggs store method.
Same kind is stored using above-mentioned 5 kinds of methods, hatched with a batch of silkworm eggs, the Second Year beginning of spring respectively,
Many experiments, as a result such as table 1：
Table 1
| Hatching success（%） | |
| Embodiment 1 | 62 |
| Embodiment 2 | 60 |
| Comparative example 1 | 48 |
| Comparative example 2 | 51 |
| Control group | 42 |
As shown in Table 1, using a kind of silkworm eggs store method provided by the invention, the hatching success of silkworm eggs is substantially increased
Rate；Using conventional cold preserving method, the hatching success of silkworm seed is only 42%, and uses the inventive method, the hatching success of silkworm seed
Rate is promoted to more than 60%, significant effect.
Claims (2)
1. a kind of silkworm eggs store method, it is characterised in that specifically include following steps：
（1）The selection of spawning paper
Before silkworm moth lays eggs, it is placed on spawning bottom portion using Bactericidal paper and is laid eggs for silkworm moth；The Bactericidal paper, specifically by with lower section
Method is made:
A takes common print paper, and use quality fraction is 2% sodium chloride solution maceration, is then dried, and is flattened；
B according to the mass fraction, takes folium artemisiae argyi 15-20 parts, north wind grass 10-15 parts, purslane 10-15 parts, toad grass 8-12 parts, sheep ear
Infusion 15-20 minutes after chrysanthemum 2-5 parts, dried orange peel 2-5 parts, the mixing of water 500-600 parts, filter cleaner, obtain soak；
C fully soaks processing of step A good printing paper 12-15 hours using soak described in step B, dries, and flattens, i.e.,
；
（2）Spawning post processing
After silkworm moth spawning, the part that silkworm seed is carried on the Bactericidal paper is torn, shone using 40 watts of ultraviolet lamps at 2.5-3 rice
Processing 5-8 minutes are penetrated, is then placed under 10-15 DEG C of lucifuge, ventilated environment and stores 15-30 days；
（3）The storage of silkworm seed
By step（3）The silkworm seed handled well refrigerates as in 0-4 DEG C of freezer.
A kind of 2. silkworm eggs store method according to claim 1, it is characterised in that step（1）The Bactericidal paper, specifically
It is made by following methods:
A takes common print paper, and use quality fraction is 2% sodium chloride solution maceration, is then dried, and is flattened；
B according to the mass fraction, takes 20 parts of folium artemisiae argyi, 15 parts of north wind grass, 15 parts of purslane, 12 parts of toad grass, 5 parts of Sheepear Inula Herb, dried orange peel 5
Infusion 20 minutes, filter cleaner, obtains soak after 600 parts of part, water mixing；
C fully soaks the good printing paper of processing of step A 15 hours using soak described in step B, dries, and flattens, produces.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710577856.9A CN107372458A (en) | 2017-07-15 | 2017-07-15 | A kind of silkworm eggs store method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710577856.9A CN107372458A (en) | 2017-07-15 | 2017-07-15 | A kind of silkworm eggs store method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107372458A true CN107372458A (en) | 2017-11-24 |
Family
ID=60339258
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710577856.9A Withdrawn CN107372458A (en) | 2017-07-15 | 2017-07-15 | A kind of silkworm eggs store method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107372458A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109588376A (en) * | 2018-12-27 | 2019-04-09 | 广西融安县金鼎制丝有限责任公司 | A kind of store method of silkworm seed |
| US20210212300A1 (en) * | 2018-06-05 | 2021-07-15 | Bühler Insect Technology Solutions Ag | Large-Scale, High Density Storage of Larvae |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1286021A (en) * | 2000-09-27 | 2001-03-07 | 苏州大学 | Method for overyear storage of silkworm ova |
| CN1286022A (en) * | 2000-09-27 | 2001-03-07 | 苏州大学 | Slightly freezing method for storing silkworm ova |
| CN1631128A (en) * | 2004-12-28 | 2005-06-29 | 张以俭 | Method for treating silkworm egg comprising shot-term refrigeration, high temperature impact and acid immersion |
| CN102318819A (en) * | 2011-08-12 | 2012-01-18 | 辽宁柞蚕丝绸科学研究院有限责任公司 | Method for preparing Antheraea Pernyi powder and Antheraea Pernyi powder thereof |
| CN104186437A (en) * | 2014-08-28 | 2014-12-10 | 安徽省农业科学院蚕桑研究所 | Laid silkworm egg breeding method |
| CN106508827A (en) * | 2016-11-08 | 2017-03-22 | 广东省农业科学院蚕业与农产品加工研究所 | Method for reducing conservation generations of non-diapause multivoltine silkworm germplasm resource and application of method |
-
2017
- 2017-07-15 CN CN201710577856.9A patent/CN107372458A/en not_active Withdrawn
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1286021A (en) * | 2000-09-27 | 2001-03-07 | 苏州大学 | Method for overyear storage of silkworm ova |
| CN1286022A (en) * | 2000-09-27 | 2001-03-07 | 苏州大学 | Slightly freezing method for storing silkworm ova |
| CN1631128A (en) * | 2004-12-28 | 2005-06-29 | 张以俭 | Method for treating silkworm egg comprising shot-term refrigeration, high temperature impact and acid immersion |
| CN102318819A (en) * | 2011-08-12 | 2012-01-18 | 辽宁柞蚕丝绸科学研究院有限责任公司 | Method for preparing Antheraea Pernyi powder and Antheraea Pernyi powder thereof |
| CN104186437A (en) * | 2014-08-28 | 2014-12-10 | 安徽省农业科学院蚕桑研究所 | Laid silkworm egg breeding method |
| CN106508827A (en) * | 2016-11-08 | 2017-03-22 | 广东省农业科学院蚕业与农产品加工研究所 | Method for reducing conservation generations of non-diapause multivoltine silkworm germplasm resource and application of method |
Non-Patent Citations (2)
| Title |
|---|
| 吴大洋: "家蚕滞育卵长期保存的研究", 《蚕业科学》 * |
| 朱江等: "家蚕卵长期保存的实用化研究", 《蚕业科学》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20210212300A1 (en) * | 2018-06-05 | 2021-07-15 | Bühler Insect Technology Solutions Ag | Large-Scale, High Density Storage of Larvae |
| CN109588376A (en) * | 2018-12-27 | 2019-04-09 | 广西融安县金鼎制丝有限责任公司 | A kind of store method of silkworm seed |
| CN109588376B (en) * | 2018-12-27 | 2021-09-21 | 融安县德源农业科技发展有限公司 | Silkworm egg preservation method |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Xiuxiang et al. | Musk deer farming in China | |
| CN107372458A (en) | A kind of silkworm eggs store method | |
| CN103583281A (en) | Control method of green mirids | |
| CN107351209A (en) | A kind of wood preservation from decay processing method | |
| CN106577354A (en) | Scale-breeding chicken farm disinfection treatment method | |
| CN109479659A (en) | A kind of cotton seeds cultivating technique for preventing and treating pest and disease damage | |
| CN206517891U (en) | Prevent and treat the device of Kiwi berry seedling root-knot nematode | |
| CN109588376B (en) | Silkworm egg preservation method | |
| CN106508741A (en) | Intercropping breeding method of loach | |
| CN110720430A (en) | Chinese bee breeding method suitable for alpine regions | |
| CN105462520A (en) | Egg repair liquid for breeding eggs and preparation method of egg repair liquid | |
| CN107279075A (en) | A kind of silkworm in spring egg hatching method | |
| WO2018233341A1 (en) | Control method for vertical transmission of tetra hybrid bombyx mori pebrine germ | |
| CN203692229U (en) | Device for breeding chelisoches morio fabricius which is natural enemy of tirathaba rufivena | |
| CN105830577A (en) | High-germination capacity disease-resistant corn seed treatment method | |
| CN107173337B (en) | Method for preventing and treating vertical propagation of micro-particle disease embryo of silkworm double cross breeder seed | |
| BR112021006177A2 (en) | mite composition and method for raising mites | |
| CN107372333A (en) | A kind of high-survival rate hatching method of duck's egg | |
| CN101711553B (en) | Feed for raising bumblebee and preparation and feeding method thereof | |
| CN110612943A (en) | Comprehensive control method for honeybee cotton worms | |
| CN114391518B (en) | Silkworm egg hatching method based on warm water immersion | |
| CN106889107A (en) | A kind of toothed oak garden disinfectant and application method | |
| Bacot et al. | The incubation period of the eggs of Haematopinus asini | |
| CN107184628A (en) | A kind of antibacterial film forming spray for animals | |
| CN108935337B (en) | Technology for raising breeding success rate and breeding number of scleroderma guani |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WW01 | Invention patent application withdrawn after publication |
Application publication date: 20171124 |
|
| WW01 | Invention patent application withdrawn after publication |