CN112300934A

CN112300934A - Porous culture plate for biological cell culture

Info

Publication number: CN112300934A
Application number: CN202011210515.6A
Authority: CN
Inventors: 浠讳华; 任仪
Original assignee: Individual
Current assignee: Individual
Priority date: 2020-11-03
Filing date: 2020-11-03
Publication date: 2021-02-02

Abstract

The invention discloses a porous culture plate for biological cell culture, which comprises a lower support plate, an upper support plate, a first rotating rod, outer culture holes and inner culture holes, wherein the left end and the right end of the lower support plate are integrally connected with the upper support plate, the outer culture holes and the inner culture holes are arranged in the upper support plate at equal intervals by taking the circle center of the first rotating rod as the circle center, the inner culture holes are arranged on the inner sides of the outer culture holes, a first cover plate is attached to the upper surface of the upper support plate, a second cover plate is attached to the inner part of the upper surface of the first cover plate, a first rotating rod is fixed to the middle of the second cover plate through a screw, and the bottom end of the first rotating rod is connected with an upper surface bearing of the first cover plate. This a porous culture plate for biological cell culture installs the second apron, and the setting through the second apron is convenient for shelter from other outer culture hole and interior culture hole when pouring into the cell, avoids external impurity to fall into other outer culture hole and interior culture hole downthehole.

Description

Porous culture plate for biological cell culture

Technical Field

The invention relates to the technical field of biological cell culture, in particular to a porous culture plate for biological cell culture.

Background

Biological cell culture means that the growth environment of cells is set through biotechnology, so that the cells are cultured in vitro, the growth and propagation conditions of the cells can be conveniently observed, the cells can be conveniently researched, a multi-hole culture plate is required to be used for placing the biological cells in the biological cell culture process, although the multi-hole culture plate in the market is various, the multi-hole culture plate has some defects in the use process, such as:

when the existing porous culture plate is used for placing cells, the cells need to be placed into a plurality of outer culture grooves and inner culture grooves in the culture plate one by one, and the outer culture grooves and the inner culture grooves are exposed outside when the cells are placed, so that external impurities can easily enter the outer culture grooves and the inner culture grooves, and the cells in the outer culture grooves and the inner culture grooves are polluted, and the later culture work is influenced;

we have now proposed a multi-well plate for biological cell culture that is capable of solving the above problems.

Disclosure of Invention

The invention aims to provide a porous culture plate for biological cell culture, which solves the problems that the prior porous culture plate in the market in the background art needs to put cells into a plurality of outer culture grooves and inner culture grooves one by one when the cells are placed and used, and the outer culture grooves and the inner culture grooves are exposed outside when the cells are placed, so that external impurities easily enter the outer culture grooves and the inner culture grooves, and the cells in the outer culture grooves and the inner culture grooves are polluted to influence the later culture work.

In order to achieve the purpose, the invention provides the following technical scheme: a multi-hole culture plate for biological cell culture comprises a lower supporting plate, an upper supporting plate, a first rotating rod, outer culture holes and inner culture holes, wherein the left end and the right end of the lower supporting plate are integrally connected with the upper supporting plate, the outer culture holes and the inner culture holes are arranged in the upper supporting plate at equal intervals by taking the circle center of the first rotating rod as the circle center, the inner sides of the outer culture holes are provided with the inner culture holes, a first cover plate is arranged on the upper surface of the upper supporting plate in a laminating manner, a second cover plate is arranged on the inner portion of the upper surface of the first cover plate in a laminating manner, a first rotating rod is fixed to the middle of the second cover plate through a screw, the bottom end of the first rotating rod is connected with an upper surface bearing of the first cover plate, fixing rods are connected to the inner portions of the left end and the right end of the first cover plate in a penetrating manner, a first, and second feed opening and storage tank have been seted up on the upper surface right side of second apron to the outside of first feed opening and second feed opening all is provided with the storage tank, baffle and second dwang have been placed to the inside of storage tank, and the one end top fix with screw of baffle has the second dwang, and the top of second dwang runs through the bearing and is connected with the second apron.

Preferably, the upper surfaces of the left end and the right end of the upper supporting plate are provided with a groove, a positioning groove and a clamping groove, the left side and the right side of the groove are provided with the positioning groove and the clamping groove, and the clamping groove is formed in the outer side of the positioning groove.

Preferably, the space homogeneous phase inside recess, constant head tank and draw-in groove three communicates, and the top of recess and constant head tank is opening column structure setting to the peak of constant head tank is higher than the peak of draw-in groove, and the draw-in groove is arc column structure setting.

Preferably, the first cover plate is arranged in a U-shaped structure, the upper surface of the first cover plate is attached to the lower surface of the second cover plate, two rows of through holes are formed in the first cover plate at equal intervals, the outer row of through holes and the outer culture holes are arranged in a one-to-one correspondence manner, and the inner row of through holes and the inner culture holes are arranged in a one-to-one correspondence manner.

Preferably, the vertical center line of the first feed opening coincides with the vertical center line of the corresponding outer culture hole below, and the space inside the first feed opening and the space inside the second feed opening are both communicated with the space inside the accommodating groove.

Preferably, the left side and the right side of the bottom end of the fixing rod are both fixed with a bump through screws, and the bumps are in clamping sliding connection with the clamping grooves.

Preferably, the diameter of the baffle plate is larger than the diameters of the first feed opening and the second feed opening, and the baffle plate and the second cover plate form a rotating structure through the second rotating rod.

Preferably, the length of the accommodating groove is greater than 2 times of the diameter of the shielding plate, and the height of the accommodating groove is smaller than the height of the first feed opening and the second feed opening.

Compared with the prior art, the invention has the beneficial effects that: the multi-hole culture plate for biological cell culture;

(1) the second cover plate is arranged, a first feed opening and a second feed opening are formed in the second cover plate, the first feed opening is sequentially overlapped and corresponds to the outer culture holes when rotating, the second feed opening is sequentially overlapped and corresponds to the inner culture holes when rotating, cells can be sequentially injected into the outer culture holes and the inner culture holes, other outer culture holes and other inner culture holes can be conveniently shielded through the second cover plate when the cells are injected, and external impurities are prevented from falling into other outer culture holes and other inner culture holes;

(2) the fixing rod is fixed, and the rotation of the fixing rod can drive the bumps on the left side and the right side of the bottom end to rotate, so that the bumps rotate into the positioning grooves from the clamping grooves, and then the bumps can be conveniently taken out from the positioning grooves with an opening shape at the upper part, so that the first cover plate is conveniently separated from the upper support plate, and a plurality of outer culture holes and inner culture holes in the upper support plate can be conveniently cleaned at a later stage;

(3) install the shielding plate, can drive the shielding plate through the rotation of second dwang and rotate for in the shielding plate rotated to first feed opening and second feed opening from the holding tank, so that the shielding plate shelters from and opens first feed opening and second feed opening.

Drawings

FIG. 1 is a schematic main sectional view of the present invention;

FIG. 2 is a schematic top view of a first cover plate according to the present invention;

FIG. 3 is a schematic top view of a second cover plate according to the present invention;

FIG. 4 is a schematic structural view of the connection between the upper support plate and the left end of the first cover plate;

FIG. 5 is a schematic top view of the connection between the fixing rod and the upper supporting plate according to the present invention;

FIG. 6 is a schematic top view of the shielding plate of the present invention;

FIG. 7 is a schematic view of the connection between the shielding plate and the second cover plate.

In the figure: 1. a lower support plate; 2. an upper support plate; 201. a groove; 202. positioning a groove; 203. a card slot; 3. a first cover plate; 4. a second cover plate; 5. a first feed opening; 6. a second feed opening; 7. a first rotating lever; 8. fixing the rod; 81. a bump; 9. a through hole; 10. an outer culture well; 11. a shielding plate; 12. a second rotating lever; 13. a containing groove; 14. an inner culture well.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Referring to fig. 1-7, the present invention provides a technical solution: a porous culture plate for biological cell culture comprises a lower support plate 1, an upper support plate 2, a groove 201, a positioning groove 202, a clamping groove 203, a first cover plate 3, a second cover plate 4, a first feed opening 5, a second feed opening 6, a first rotating rod 7, a fixing rod 8, a bump 81, a through hole 9, an outer culture hole 10, a baffle plate 11, a second rotating rod 12, a containing groove 13 and an inner culture hole 14, wherein the left end and the right end of the lower support plate 1 are integrally connected with the upper support plate 2, the inner part of the upper support plate 2 is provided with the outer culture hole 10 and the inner culture hole 14 at equal intervals by taking the circle center of the first rotating rod 7 as the circle center, the inner side of the outer culture hole 10 is provided with the inner culture hole 14, the upper surface of the upper support plate 2 is provided with the first cover plate 3 in an attaching manner, the inner part of the upper surface of the first cover plate 3 is provided with the second cover plate 4 in an attaching manner, the bottom of first dwang 7 is connected with the upper surface bearing of first apron 3, the inside bearing that all runs through in both ends of the left and right sides of first apron 3 is connected with dead lever 8, first feed opening 5 and storage tank 13 have been seted up on the left of the upper surface of second apron 4, and second feed opening 6 and storage tank 13 have been seted up on the upper surface right side of second apron 4, and the outside of first feed opening 5 and second feed opening 6 all is provided with storage tank 13, baffle 11 and second dwang 12 have been placed to storage tank 13's inside, and the one end top fix with screw of baffle 11 has second dwang 12, and the top of second dwang 12 runs through the bearing and is connected with second apron 4.

The upper surfaces of the left end and the right end of the upper supporting plate 2 are respectively provided with a groove 201, a positioning groove 202 and a clamping groove 203, the left side and the right side of the groove 201 are respectively provided with the positioning groove 202 and the clamping groove 203, and the outer side of the positioning groove 202 is provided with the clamping groove 203, so that the upper surfaces of the left end and the right end of the upper supporting plate 2 are respectively provided with the groove 201, the positioning groove 202 and the clamping groove 203, the left side and the right side of the groove 201 are respectively provided with the positioning groove 202 and the clamping groove 203, and the outer side of the positioning groove 202 is provided with;

the groove 201, the space inside the positioning groove 202 and the space inside the clamping groove 203 are communicated, the upper portions of the groove 201 and the positioning groove 202 are both arranged in an open structure, the highest point of the positioning groove 202 is higher than that of the clamping groove 203, the clamping groove 203 is arranged in an arc-shaped structure, the space inside the positioning groove 202 and the space inside the clamping groove 203 are communicated through the groove 201, so that the lug 81 can be rotated into the clamping groove 203 from the inside of the positioning groove 202 for clamping and fixing in the later period, the upper portions of the groove 201 and the positioning groove 202 are arranged in an open structure, so that the fixing rod 8 and the upper supporting plate 2 can be separated in the later period, and the upper supporting plate 2 and the first cover plate 3 can;

the first cover plate 3 is arranged in a U-shaped structure, the upper surface of the first cover plate 3 is attached to the lower surface of the second cover plate 4, two rows of through holes 9 are formed in the first cover plate 3 at equal intervals, the outer rows of through holes 9 are arranged in one-to-one correspondence with the outer culture holes 10, the inner rows of through holes 9 are arranged in one-to-one correspondence with the inner culture holes 14, the two rows of through holes 9 are formed in the first cover plate 3 at equal intervals, the outer rows of through holes 9 are arranged in one-to-one correspondence with the outer culture holes 10, and the inner rows of through holes 9 are arranged in one-to-one correspondence with the inner culture holes 14, so that cells can be conveniently injected into the outer culture holes 10 and the inner culture holes 14 through the through holes 9;

the vertical center line of the first feed opening 5 coincides with the vertical center line of the corresponding outer culture hole 10 below, and the spaces inside the first feed opening 5 and the second feed opening 6 are both communicated with the space inside the accommodating groove 13, so that the cells can be conveniently injected into the outer culture hole 10 through the matching of the first feed opening 5 and the through hole 9 by the design that the vertical center line of the first feed opening 5 coincides with the vertical center line of the corresponding outer culture hole 10 below;

the left side and the right side of the bottom end of the fixed rod 8 are both provided with the convex blocks 81 in a screw fixing manner, and the convex blocks 81 are in clamping sliding connection with the clamping grooves 203, so that the convex blocks 81 are in clamping sliding connection with the clamping grooves 203 conveniently;

the diameter of the shielding plate 11 is larger than the diameters of the first feed opening 5 and the second feed opening 6, the shielding plate 11 and the second cover plate 4 form a rotating structure through the second rotating rod 12, and then the diameter of the shielding plate 11 is larger than the diameters of the first feed opening 5 and the second feed opening 6, so that the shielding plate 11 well shields, closes and opens the first feed opening 5 and the second feed opening 6;

the length of storage tank 13 is greater than 2 times of shielding plate 11 diameter, and the height of storage tank 13 is less than the height of first feed opening 5 and second feed opening 6, and the length through storage tank 13 is greater than 2 times of shielding plate 11 diameter to the later stage shielding plate 11 is fine in storage tank 13 rotates.

The working principle is as follows: when the multi-hole culture plate for biological cell culture is used, firstly, as shown in fig. 1, the whole multi-hole culture plate is placed in a proper working area, then the whole multi-hole culture plate can be used, when a certain amount of cells are required to be injected into a plurality of outer culture holes 10 in the multi-hole culture plate, at this time, as shown in fig. 1-4 and fig. 6-7, a second rotating rod 12 near a first discharging port 5 is manually rotated, so that the second rotating rod 12 drives a corresponding shielding plate 11 to rotate into a containing groove 13, thereby the first discharging port 5 is opened, then the first rotating rod 7 is manually rotated, so that the first rotating rod 7 drives a second cover plate 4 to rotate by taking the circle center of the first rotating rod 7 as the circle center, the second cover plate 4 drives the first discharging port 5 to rotate, when the first discharging port 5 is overlapped with the corresponding outer culture hole 10, at this time, the cells are injected into the corresponding outer culture holes 10 through a discharge port 5 and the through hole 9, and other outer culture holes 10 are covered by the second cover plate 4 when the cells are injected, so that external impurities are prevented from entering the other outer culture holes 10;

then, the second cover plate 4 is continuously rotated to sequentially inject the cells into other outer culture holes 10, when the cells are required to be injected into the inner culture hole 14, the structure inside the second feed opening 6 is equal to the structure inside the first feed opening 5, and the shielding plates 11 are arranged in the second feed opening 6 and the first feed opening 5, so that the operation can be performed in the same way as described above, and then external impurities are prevented from polluting other outer culture holes 10 and inner culture holes 14 when the cells are injected, so that the cells are favorably injected, the operation is convenient, and the structure is simple;

after the use, when cells in the plurality of outer culture holes 10 and the plurality of inner culture holes 14 need to be collected and cleaned, as shown in fig. 1 and fig. 4-5, the fixing rod 8 is manually and sequentially rotated, so that the fixing rod 8 drives the protruding blocks 81 at the left side and the right side of the bottom to rotate, and further the protruding blocks 81 rotate into the positioning grooves 202 from the corresponding clamping grooves 203, and then the fixing rod 8 can be pulled upwards, so that the fixing rod 8 drives the protruding blocks 81 to be pulled out from the positioning grooves 202 with the upper parts being open, and further the first cover plate 3 is conveniently separated from the upper support plate 2, so that the outer culture holes 10 and the inner culture holes 14 in the upper support plate 2 can be cleaned at a later stage.

Although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that various changes in the embodiments and/or modifications of the invention can be made, and equivalents and modifications of some features of the invention can be made without departing from the spirit and scope of the invention.

Claims

1. A porous culture plate for biological cell culture, comprising a lower support plate (1), an upper support plate (2), a first rotating rod (7), an outer culture hole (10) and an inner culture hole (14), characterized in that: the left end and the right end of the lower supporting plate (1) are integrally connected with the upper supporting plate (2), the inner part of the upper supporting plate (2) is provided with an outer culture hole (10) and an inner culture hole (14) at equal intervals by taking the circle center of the first rotating rod (7) as the circle center, the inner side of the outer culture hole (10) is provided with the inner culture hole (14), the upper surface of the upper supporting plate (2) is provided with a first cover plate (3) in a fitting manner, the inner part of the upper surface of the first cover plate (3) is provided with a second cover plate (4) in a fitting manner, the middle of the second cover plate (4) is fixed with the first rotating rod (7) through a screw, the bottom end of the first rotating rod (7) is connected with an upper surface bearing of the first cover plate (3), the inner parts of the left end and the right end of the first cover plate (3) are connected with a fixing rod (8) through a bearing, the left side of the upper surface of, and second feed opening (6) and storage tank (13) have been seted up on the upper surface right side of second apron (4) to the outside of first feed opening (5) and second feed opening (6) all is provided with storage tank (13), baffle plate (11) and second dwang (12) have been placed to the inside of storage tank (13), and the one end top fix with screw of baffle plate (11) has second dwang (12), and the top through bearing of second dwang (12) is connected with second apron (4).

2. A multi-well culture plate for biological cell culture according to claim 1, wherein: go up backup pad (2) the left and right sides both ends upper surface and all seted up recess (201), constant head tank (202) and draw-in groove (203), and the left and right sides of recess (201) all is provided with constant head tank (202) and draw-in groove (203) to the outside of constant head tank (202) is provided with draw-in groove (203).

3. A multi-well culture plate for biological cell culture according to claim 2, wherein: the inner space of the groove (201), the positioning groove (202) and the clamping groove (203) are communicated, the upper portions of the groove (201) and the positioning groove (202) are both in an opening structure, the highest point of the positioning groove (202) is higher than the highest point of the clamping groove (203), and the clamping groove (203) is in an arc-shaped structure.

4. A multi-well culture plate for biological cell culture according to claim 1, wherein: first apron (3) are the setting of "U" shape structure, and the upper surface of first apron (3) laminates mutually with the lower surface of second apron (4) to two rows of through-holes (9) have been seted up to the inside equidistant of first apron (3), and outer through-hole (9) of arranging are the one-to-one setting with outer cultivation hole (10), and interior through-hole (9) of arranging are the one-to-one setting with interior cultivation hole (14).

5. A multi-well culture plate for biological cell culture according to claim 1, wherein: the vertical center line of the first feed opening (5) coincides with the vertical center line of the outer culture hole (10) corresponding to the lower part, and the space inside the first feed opening (5) and the space inside the second feed opening (6) are communicated with the space inside the accommodating groove (13).

6. A multi-well culture plate for biological cell culture according to claim 2, wherein: the left side and the right side of the bottom end of the fixed rod (8) are both fixed with a bump (81) through screws, and the bump (81) is connected with the clamping groove (203) in a clamping and sliding manner.

7. A multi-well culture plate for biological cell culture according to claim 1, wherein: the diameter of the baffle plate (11) is larger than the diameters of the first feed opening (5) and the second feed opening (6), and the baffle plate (11) forms a rotating structure with the second cover plate (4) through the second rotating rod (12).

8. A multi-well culture plate for biological cell culture according to claim 1, wherein: the length of the containing groove (13) is larger than 2 times of the diameter of the shielding plate (11), and the height of the containing groove (13) is smaller than the height of the first feed opening (5) and the second feed opening (6).