CN111296410A - Cell freezing device for monoclonal antibody preparation - Google Patents
Cell freezing device for monoclonal antibody preparation Download PDFInfo
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- CN111296410A CN111296410A CN202010158311.6A CN202010158311A CN111296410A CN 111296410 A CN111296410 A CN 111296410A CN 202010158311 A CN202010158311 A CN 202010158311A CN 111296410 A CN111296410 A CN 111296410A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0236—Mechanical aspects
- A01N1/0242—Apparatuses, i.e. devices used in the process of preservation of living parts, such as pumps, refrigeration devices or any other devices featuring moving parts and/or temperature controlling components
- A01N1/0252—Temperature controlling refrigerating apparatus, i.e. devices used to actively control the temperature of a designated internal volume, e.g. refrigerators, freeze-drying apparatus or liquid nitrogen baths
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Abstract
The invention discloses a cell freezing device for preparing monoclonal antibody, which comprises a support frame and a freezing cabinet, wherein the freezing cabinet is arranged at the bottom in the support frame, a first motor is arranged on the support frame, and the end part of an output shaft of the first motor is connected with a screw rod. According to the invention, the output shaft of the second motor drives the gear to rotate, the gear is meshed with the rack to drive the connecting plate to move horizontally, the sliding block on the connecting plate slides along the sliding rail at the bottom of the upper lifting plate, so that the freezing cabinet is moved out of the supporting frame, the telescopic cylinder piston rods on two sides of the lower lifting plate push the right-angle clamping block, the right-angle clamping block is not clamped with the top surface fixing frame, the freezing frame can be taken down, cells are added into the test tube on the freezing frame, then the right-angle clamping block fixes the top surface fixing frame on the freezing frame, the freezing frame enters the freezing cabinet, the cabinet door is closed, and the freezing cabinet freezes the cells in the test tube on the freezing frame.
Description
Technical Field
The invention relates to the technical field of monoclonal antibody preparation, in particular to a cell freezing device for preparing monoclonal antibody.
Background
Monoclonal antibody technology is an important work in modern life science research, and plays an indispensable role in the structural and functional research of genes and proteins. At present, the monoclonal antibody has two preparation methods in vivo and in vitro, and can be used only by freezing cells.
Patent document (201510038391.0) discloses a cell freezing device and method, which is constructed by providing a rotary driving mechanism in a reaction chamber of the freezing device, the bottled feed liquid is rotated while being frozen, the feed liquid is shaken up to be heated uniformly, the central area of the feed liquid in the bottle is in a hollow state, the cold and heat exchange is carried out more quickly, thereby not only achieving the effect of quick freezing, but also ensuring the stability and the uniformity of the feed liquid and achieving better unfreezing effect, the device is only improved on the whole freezing and unfreezing process of cell freezing, but is very inconvenient in the aspects of cell addition and the inlet and outlet of a freezing frame, and firstly, the test tube of this cell refrigerating plant, freezing frame business turn over need artificial completion to rocking degree too big can spill or destroy the cell sap in the business turn over process of test tube, freezing frame.
Disclosure of Invention
The invention aims to provide a cell freezing device for preparing monoclonal antibody, which solves the following technical problems: (1) the screw rod is driven to rotate by an output shaft of a third motor on the lower surface of the connecting plate, the screw rod is matched with the nut to drive a nut cover block to horizontally move, the nut cover block drives a first moving shaft to horizontally move, so as to drive first slider link blocks on two sides of the first moving shaft to slide on a first guide rail, so that the first jacking channel steel and a second jacking channel steel are rotated, and further, a lower lifting plate is driven to ascend, the second jacking channel steel drives second slider link blocks on two sides of a second moving shaft to slide on a second guide rail, the first jacking channel steel and the second jacking channel steel are mutually rotated, through the matching design of the third motor, the screw rod and the nut, the first slider link block on the first moving shaft slides on the first guide rail, so that the rotating process of the first jacking channel steel and the second jacking channel steel is more stable, and simultaneously, the ascending of the upper lifting plate is matched, so that the freezing frame can complete two, the second motor output shaft drives the gear to rotate, the gear meshing rack drives the connecting plate to move in the horizontal direction, a sliding block on the connecting plate slides along a sliding rail at the bottom of the upper lifting plate, and then the freezer is moved out of the supporting frame; (2) the fixing frame on the top surface is clamped through the right-angle clamping block to drive the freezing frame to ascend, the freezing frame ascends from the freezing frame groove, the positioning rod ascends from the rod groove, and then the freezing frame is lifted up, the freezing frame is moved out of the freezing cabinet, through the arrangement, the outer wall of the positioning rod is in movable contact with the inner wall of the rod groove, the freezing frame is effectively prevented from shaking in the process of entering and exiting the freezing cabinet, so that cells in a test tube are prevented from being damaged due to shaking, and the technical problem that the cells in the test tube are damaged or cell sap is spilled due to shaking in the process of entering and exiting the freezing frame in; (3) through the design of support frame, all set up cell refrigerating plant on the support frame, effectively reduce whole cell refrigerating plant's area, cell refrigerating plant area is big among the solution prior art, the technical problem of inconvenient use.
The purpose of the invention can be realized by the following technical scheme:
the utility model provides a cell refrigeration device for monoclonal antibody preparation, includes support frame, freezer chest installs the bottom in the support frame, install first motor on the support frame, first motor output shaft end connection has the lead screw, the lead screw outer peripheral face rotates and is provided with interior silk cover, interior silk cover is installed on last lifter plate, fixed surface installs the mounting panel on going up the lifter plate, fixed surface installs the second motor on the mounting panel, second motor output shaft runs through last lifter plate and tip and installs the gear, the gear sets up in last lifter plate below, gear one side meshing has the rack, rack fixed mounting is in the connecting plate top, the lifter plate is connected in the connecting plate sliding, the connecting plate below is provided with down the lifter plate, it is provided with first jacking channel-section steel, channel-section steel to rotate between connecting plate and the lower lifter plate, The first jacking channel steel and the second jacking channel steel are rotatably connected;
four telescopic cylinder are installed to lower lifter plate bilateral symmetry, the right angle fixture block is installed to telescopic cylinder piston rod tip, the fixed frame both sides of four right angle fixture block activity joint top surface, the fixed frame of top surface is installed on freezing the frame, freezing cabinet top one side is rotated and is installed the cabinet door, freezing frame is rectangular array form and installs a plurality of test tubes, freezing frame bottom equidistant three locating lever that is fixed with, freezing cabinet cavity has been seted up and has been frozen the frame groove, freezing cabinet cavity bottom sets up the pole groove of three equidistant setting, freezing frame groove communicates with the pole groove each other, locating lever outer wall and pole inslot wall active contact, freezing frame lateral wall and freezing frame inslot wall active contact.
Furthermore, two rod sleeves are further installed on the upper lifting plate, the rod sleeves are slidably sleeved on the vertical rods, the two vertical rods are respectively arranged on two sides of the screw rod, and through holes are formed in the upper lifting plate.
Furthermore, the second motor output shaft movably penetrates through the through hole, rod seats are fixedly mounted at two ends of the vertical rod, and the rod seats are fixed on the supporting frame.
Further, two back timber are installed at the support frame top, and two back timbers are parallel to each other, and install first motor through the motor cabinet on two back timbers.
Furthermore, two sliding rails are fixedly mounted on the lower surface of the upper lifting plate, sliding blocks are connected to the surfaces of the sliding rails in a sliding mode, and the sliding blocks are mounted on the upper surface of the connecting plate.
Further, a third motor is arranged on the lower surface of the connecting plate, a screw rod is arranged at the end part of an output shaft of the third motor, a nut is rotatably arranged on the outer peripheral surface of the screw rod, a nut cover block is arranged on the nut, one end of the screw rod, far away from the third motor, is rotatably connected with a fixed seat, the fixed seat is fixedly arranged on the lower surface of the connecting plate, a first moving shaft penetrates through the nut cover block, the first moving shaft penetrates through the nut cover block in a rotating manner, two first bearing seats are fixedly arranged on the lower surface of the connecting plate, two ends of the first moving shaft are respectively and rotatably connected with a first sliding block and link block which is arranged on the first guide rail in a sliding way, the first guide rail is fixedly arranged on the lower surface of the connecting plate, the two second guide rails are arranged on the upper surface of the lower lifting plate, and the second slide block and link block is slidably arranged on the second guide rails.
Further, it installs two first position sleeves, two to be close to both ends position on the first removal axle rotate between the first bearing frame and be provided with first rotation axis, be close to both ends position on the first rotation axis and install two second position sleeves, rotate between two second slider chain link pieces and set up the second and remove the axle, the second removes epaxial two third position sleeves of installing near both ends position that is close to of epaxial, two second bearing frames are still installed to lower lifter plate upper surface, rotate between two second bearing frames and be provided with the second axis of rotation, it installs two fourth position sleeves to be close to both ends position in the second axis of rotation, it is provided with first jacking channel-section steel to rotate between first position sleeve and the fourth position sleeve, it is provided with second jacking channel-section steel to rotate between second position sleeve and the third position sleeve.
Further, the working process of the refrigerating device is as follows:
the method comprises the following steps: the output shaft of the first motor drives the screw rod to rotate, the screw rod is matched with the inner screw sleeve to drive the upper lifting plate to ascend, the rod sleeve on the upper lifting plate ascends along the vertical rod, the upper lifting plate drives the connecting plate to ascend, meanwhile, the output shaft of the third motor on the lower surface of the connecting plate drives the screw to rotate, the screw is matched with the nut to drive the nut cover block to horizontally move, the nut cover block drives the first moving shaft to horizontally move, thereby driving the first slider link blocks at both sides of the first moving shaft to slide on the first guide rail, and further rotating the first jacking channel steel and the second jacking channel steel, the lower lifting plate is driven to ascend, the second jacking channel steel drives the second sliding block and link blocks on two sides of the second moving shaft to slide on the second guide rail, the top surface fixing frame is clamped by the right-angle clamping block to drive the freezing rack to ascend, the freezing rack ascends from the freezing rack groove, the positioning rod ascends from the rod groove, the freezing rack is further lifted, and the freezing rack is moved out of the freezing cabinet;
step two: second motor output shaft drives the gear and rotates, gear engagement rack drives the connecting plate horizontal direction and removes, the slide rail of the last lifter plate bottom of slider edge on the connecting plate slides, and then shift out the support frame with the freezer, the telescopic cylinder piston rod of lower lifter plate both sides promotes the right angle fixture block, the fixed frame no longer joint of right angle fixture block and top surface, can take off the freezing frame, add the cell in the test tube on freezing the frame, then the right angle fixture block is fixed to the fixed frame of top surface on freezing the frame, freezing in the frame gets into the freezer, close the cabinet door, the cell in the test tube is frozen to freezing the frame.
The invention has the beneficial effects that:
(1) according to the cell freezing device for preparing the monoclonal antibody, the output shaft of the first motor drives the screw rod to rotate through the matching design of the first motor, the screw rod and the vertical rod, the screw rod is matched with the inner screw sleeve to drive the upper lifting plate to ascend, the rod sleeve on the upper lifting plate ascends along the vertical rod, and the upper lifting plate drives the connecting plate to ascend, so that the whole freezing frame stably ascends;
(2) a third motor output shaft on the lower surface of the connecting plate drives a screw rod to rotate, the screw rod is matched with a nut to drive a nut cover block to horizontally move, the nut cover block drives a first moving shaft to horizontally move so as to drive first slider link blocks on two sides of the first moving shaft to slide on a first guide rail, so that first jacking channel steel and second jacking channel steel rotate, and further drives the lower lifting plate to ascend, the second jacking channel steel drives second slider link blocks on two sides of a second moving shaft to slide on a second guide rail, the first jacking channel steel and the second jacking channel steel mutually rotate, through the matching design of a third motor, the screw rod and the nut, a first slider link block on the first moving shaft slides on the first guide rail, so that the rotating process of the first jacking channel steel and the second jacking channel steel is more stable, and simultaneously, the ascending of the upper lifting plate is matched, so that the freezing frame can complete two ascending processes, the output shaft of the second motor drives the gear to rotate, the gear is meshed with the rack to drive the connecting plate to move in the horizontal direction, the sliding block on the connecting plate slides along the sliding rail at the bottom of the upper lifting plate, and then the freezer is moved out of the supporting frame;
(3) the fixed frame of right angle fixture block centre gripping top surface drives freezing frame and rises, freezing frame rises from freezing frame inslot, the locating lever rises from the pole groove, and then mention freezing frame, freezing frame shifts out from freezing the cabinet, through this setting, locating lever outer wall and pole inslot wall movable contact, it rocks to prevent effectively that the freezing frame from taking place in the business turn over process on freezing the cabinet, thereby avoid causing the damage because of rocking the cell in to the test tube, setting through the fixed frame of top surface, the telescopic cylinder that lower lifter plate both sides set up can fix the fixed frame of top surface on freezing the frame through the right angle fixture block, freezing frame is put at freezing frame inslot back, telescopic cylinder cooperation right angle fixture block cancellation is fixed to the top surface, lower lifter plate, the fixed frame of rising lifter plate rises to the top, the right angle fixture block does not influence the switch of cabinet door, it can freeze the cell in.
Drawings
The invention will be further described with reference to the accompanying drawings.
FIG. 1 is a schematic diagram showing the construction of a cell freezing apparatus for monoclonal antibody production according to the present invention;
FIG. 2 is a bottom view of the upper lifter plate of the present invention;
FIG. 3 is a top view of the upper lifter plate of the present invention;
FIG. 4 is a top view of the web of the present invention;
FIG. 5 is a bottom view of the web of the present invention;
FIG. 6 is a top view of the lower lifter plate of the present invention;
FIG. 7 is an installation view of a first jacking channel steel and a second jacking channel steel of the present invention;
FIG. 8 is an internal structural view of the freezer of the present invention;
FIG. 9 is a block diagram of the freezer rack of the present invention;
FIG. 10 is a top view of a freezer rack
In the figure: 1. a support frame; 2. a freezer cabinet; 3. a top beam; 5. a first motor; 6. an upper lifting plate; 61. an inner thread sleeve; 62. a rod sleeve; 63. a through hole; 7. mounting a plate; 8. a second motor; 9. a gear; 10. a slide rail; 11. a screw rod; 12. erecting a rod; 121. a rod seat; 13. a connecting plate; 131. a rack; 14. a slider; 15. a first guide rail; 16. a first slider link block; 17. a first moving axis; 18. a first positioning sleeve; 19. a nut; 191. a nut cover block; 20. a screw; 21. a fixed seat; 22. a third motor; 23. a first bearing housing; 24. a first rotating shaft; 25. a second positioning sleeve; 26. a lower lifting plate; 27. a second guide rail; 28. a second slider link block; 29. a second moving axis; 30. a third positioning sleeve; 31. a second bearing housing; 32. a second rotating shaft; 33. a fourth positioning sleeve; 34. a first jacking channel steel; 35. second jacking channel steel; 36. a telescopic cylinder; 37. a right-angle fixture block; 38. a freezing rack; 39. a top surface fixing frame; 41. a freezer bay; 42. positioning a rod; 43. a rod groove; 44. a cabinet door; 45. test tubes.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Referring to fig. 1-10, the present invention is a cell freezing device for monoclonal antibody preparation, including a support frame 1 and a freezer cabinet 2, the freezer cabinet 2 is installed at the bottom of the support frame 1, a first motor 5 is installed on the support frame 1, an output shaft of the first motor 5 is connected to a lead screw 11, an inner screw sleeve 61 is rotatably installed on the outer circumferential surface of the lead screw 11, the inner screw sleeve 61 is installed on an upper lifting plate 6, an installation plate 7 is fixedly installed on the upper surface of the upper lifting plate 6, a second motor 8 is installed on the upper surface of the installation plate 7, an output shaft of the second motor 8 penetrates through the upper lifting plate 6 and is installed at the end thereof with a gear 9, the gear 9 is installed below the upper lifting plate 6, one side of the gear 9 is engaged with a rack 131, the rack 131 is fixedly installed on the top of a connection plate 13, the connection plate 13 is slidably connected to the upper lifting plate 6, a lower lifting plate 26 is, The second jacking channel steel 35 is rotatably connected between the first jacking channel steel 34 and the second jacking channel steel 35;
four telescopic cylinder 36 are installed to 26 bilateral symmetry of lower lifter plate, right angle fixture block 37 is installed to telescopic cylinder 36 piston rod tip, the fixed 39 both sides of top surface of four right angle fixture block 37 activity joint, the fixed frame 39 of top surface is installed on freezing frame 38, 2 top one sides of freezing cabinet are rotated and are installed cabinet door 44, freezing frame 38 is the rectangular array form and installs a plurality of test tubes 45, freezing frame 38 bottom equidistant three locating lever 42 that is fixed with, freezing frame groove 41 has been seted up to 2 inner chambers of freezing cabinet, the pole groove 43 that three equidistant setting was seted up is seted up to 2 inner chambers of freezing cabinet bottom, freezing frame groove 41 communicates with pole groove 43 each other, locating lever 42 outer wall and pole groove 43 inner wall swing joint, freezing frame 38 lateral wall and freezing frame groove 41 inner wall swing joint.
Specifically, two rod sleeves 62 are further mounted on the upper lifting plate 6, the rod sleeves 62 are slidably sleeved on the vertical rods 12, the two vertical rods 12 are respectively arranged on two sides of the screw rod 11, and through holes 63 are formed in the upper lifting plate 6. The output shaft of the second motor 8 movably penetrates through the through hole 63, the two ends of the vertical rod 12 are fixedly provided with rod seats 121, and the rod seats 121 are fixed on the support frame 1. Two back timber 3 are installed at support frame 1 top, and two back timber 3 are parallel to each other, and install first motor 5 through the motor cabinet on two back timber 3. Two sliding rails 10 are fixedly mounted on the lower surface of the upper lifting plate 6, a sliding block 14 is connected to the surface of each sliding rail 10 in a sliding manner, and the sliding block 14 is mounted on the upper surface of the connecting plate 13. The lower surface of the connecting plate 13 is provided with a third motor 22, the end part of an output shaft of the third motor 22 is provided with a screw rod 20, the peripheral surface of the screw rod 20 is rotatably provided with a nut 19, the nut 19 is provided with a nut cover block 191, one end of the screw rod 20, which is far away from the third motor 22, is rotatably connected with a fixed seat 21, the fixed seat 21 is fixedly arranged on the lower surface of the connecting plate 13, a first moving shaft 17 penetrates through the nut cover block 191, the first moving shaft 17 rotatably penetrates through the nut cover block 191, the lower surface of the connecting plate 13 is also fixedly provided with two first bearing seats 23, two ends of the first moving shaft 17 are rotatably connected with first slider link blocks 16, the first slider link blocks 16 are slidably arranged on the first guide rail 15, the first guide rail 15 is fixedly arranged on the lower surface of the connecting plate 13, the upper surface of the lower lifting plate 26. Two first positioning sleeves 18 are installed on the first moving shaft 17 close to the two ends, a first rotating shaft 24 is rotatably arranged between the two first bearing seats 23, two second positioning sleeves 25 are installed on the first rotating shaft 24 close to the two ends, a second moving shaft 29 is rotatably arranged between the two second slider link blocks 28, two third positioning sleeves 30 are installed on the second moving shaft 29 close to the two ends, two second bearing seats 31 are further installed on the upper surface of the lower lifting plate 26, a second rotating shaft 32 is rotatably arranged between the two second bearing seats 31, two fourth positioning sleeves 33 are installed on the second rotating shaft 32 close to the two ends, a first jacking channel steel 34 is rotatably arranged between the first positioning sleeve 18 and the fourth positioning sleeve 33, and a second jacking channel steel 35 is rotatably arranged between the second positioning sleeve 25 and the third positioning sleeve 30.
Referring to fig. 1-10, the cell freezing device for monoclonal antibody production of this embodiment works as follows:
the method comprises the following steps: an output shaft of the first motor 5 drives the screw rod 11 to rotate, the screw rod 11 is matched with the inner screw sleeve 61 to drive the upper lifting plate 6 to ascend, the rod sleeve 62 on the upper lifting plate 6 ascends along the vertical rod 12, the upper lifting plate 6 drives the connecting plate 13 to ascend, the first jacking channel steel 34 and the second jacking channel steel 35 rotate to further drive the lower lifting plate 26 to ascend, the top fixing frame 39 is clamped by the right-angle clamping block 37 to drive the freezing frame 38 to ascend, the freezing frame 38 ascends from the freezing frame groove 41, the positioning rod 42 ascends from the rod groove 43 to further lift the freezing frame 38, and the freezing frame 38 is moved out of the freezing cabinet 2;
step two: the output shaft of the second motor 8 drives the gear 9 to rotate, the gear 9 meshes the rack 131 to drive the connecting plate 13 to move in the horizontal direction, the slide block 14 on the connecting plate 13 slides along the slide rail 10 at the bottom of the upper lifting plate 6, and then the freezer 2 is moved out of the support frame 1, the piston rods of the telescopic cylinders 36 on two sides of the lower lifting plate 26 push the right-angle fixture block 37, the right-angle fixture block 37 is not clamped with the top surface fixing frame 39, the freezer frame 38 can be taken down, cells are added into the test tube 45 on the freezer frame 38, then the right-angle fixture block 37 is fixed to the top surface fixing frame 39 on the freezer frame 38, the freezer frame 38 enters the freezer 2, the cabinet door 44 is closed, and the freezer cabinet 2 freezes the.
The foregoing is merely exemplary and illustrative of the present invention and various modifications, additions and substitutions may be made by those skilled in the art to the specific embodiments described without departing from the scope of the invention as defined in the following claims.
Claims (8)
1. The cell freezing device for preparing the monoclonal antibody is characterized by comprising a support frame (1) and a freezing cabinet (2), wherein the freezing cabinet (2) is arranged at the bottom in the support frame (1), a first motor (5) is arranged on the support frame (1), the end part of an output shaft of the first motor (5) is connected with a lead screw (11), an inner screw sleeve (61) is rotatably arranged on the outer peripheral surface of the lead screw (11), the inner screw sleeve (61) is arranged on an upper lifting plate (6), a mounting plate (7) is fixedly arranged on the upper surface of the upper lifting plate (6), a second motor (8) is arranged on the upper surface of the mounting plate (7), an output shaft of the second motor (8) penetrates through the upper lifting plate (6) and the end part is provided with a gear (9), the gear (9) is arranged below the upper lifting plate (6), one side of the gear (9) is meshed with a rack (131), the rack (131) is fixedly arranged at the top of the connecting plate (13), the connecting plate (13) is connected with the upper lifting plate (6) in a sliding mode, the lower lifting plate (26) is arranged below the connecting plate (13), a first lifting channel steel (34) and a second lifting channel steel (35) are rotatably arranged between the connecting plate (13) and the lower lifting plate (26), and the first lifting channel steel (34) and the second lifting channel steel (35) are rotatably connected;
four telescopic cylinders (36) are symmetrically installed on two sides of the lower lifting plate (26), a right-angle clamping block (37) is installed at the end part of a piston rod of each telescopic cylinder (36), two sides of a top surface fixing frame (39) are movably clamped by the four right-angle clamping blocks (37), the top surface fixing frame (39) is installed on the freezing frame (38), a cabinet door (44) is rotatably installed on one side of the top of the freezing cabinet (2), the freezing frame (38) is in a rectangular array shape and is provided with a plurality of test tubes (45), three positioning rods (42) are fixed at the bottom of the freezing frame (38) at equal intervals, a freezing frame groove (41) is formed in the inner cavity of the freezing cabinet (2), three rod grooves (43) which are arranged at equal intervals are formed in the bottom of the inner cavity of the freezing cabinet (2), the freezing frame groove (41) is mutually communicated with the rod grooves (43), and, the side wall of the freezing rack (38) is movably contacted with the inner wall of the freezing rack groove (41).
2. The cell freezing device for preparing monoclonal antibody according to claim 1, wherein the upper lifting plate (6) is further provided with two rod sleeves (62), the rod sleeves (62) are slidably sleeved on the vertical rods (12), the two vertical rods (12) are respectively arranged on two sides of the screw rod (11), and the upper lifting plate (6) is provided with a through hole (63).
3. The cell freezing device for preparing monoclonal antibody according to claim 2, wherein the output shaft of the second motor (8) movably penetrates through the through hole (63), the two ends of the vertical rod (12) are fixedly provided with rod seats (121), and the rod seats (121) are fixed on the support frame (1).
4. The cell freezing device for preparing monoclonal antibody according to claim 1, wherein two top beams (3) are installed on the top of the support frame (1), the two top beams (3) are parallel to each other, and the first motor (5) is installed on the two top beams (3) through a motor base.
5. The cell freezing device for preparing monoclonal antibody according to claim 1, wherein two sliding rails (10) are fixedly installed on the lower surface of the upper lifting plate (6), a sliding block (14) is connected to the surface of each sliding rail (10) in a sliding manner, and the sliding block (14) is installed on the upper surface of the connecting plate (13).
6. The cell freezing device for preparing monoclonal antibody according to claim 1, wherein a third motor (22) is installed on the lower surface of the connecting plate (13), a screw rod (20) is installed at the end of the output shaft of the third motor (22), a nut (19) is installed on the outer circumferential surface of the screw rod (20) in a rotating manner, a nut cover block (191) is installed on the nut (19), one end of the screw rod (20) far away from the third motor (22) is connected with a fixed seat (21) in a rotating manner, the fixed seat (21) is fixedly installed on the lower surface of the connecting plate (13), a first moving shaft (17) penetrates through the nut cover block (191), the first moving shaft (17) penetrates through the nut cover block (191) in a rotating manner, two first bearing seats (23) are further fixedly installed on the lower surface of the connecting plate (13), and first slider link blocks (16) are rotatably connected to both ends of the first moving shaft (17), first slider chain joint piece (16) slidable mounting is on first guide rail (15), first guide rail (15) fixed mounting is in connecting plate (13) lower surface, lifter plate (26) upper surface mounting has two second guide rails (27) down, slidable mounting has second slider chain joint piece (28) on second guide rail (27).
7. The cell freezing device for monoclonal antibody preparation according to claim 6, wherein two first positioning sleeves (18) are mounted on the first moving shaft (17) near two ends, a first rotating shaft (24) is rotatably disposed between the two first bearing blocks (23), two second positioning sleeves (25) are mounted on the first rotating shaft (24) near two ends, a second moving shaft (29) is rotatably disposed between the two second slider link blocks (28), two third positioning sleeves (30) are mounted on the second moving shaft (29) near two ends, two second bearing blocks (31) are further mounted on the upper surface of the lower lifting plate (26), a second rotating shaft (32) is rotatably disposed between the two second bearing blocks (31), and two fourth positioning sleeves (33) are mounted on the second rotating shaft (32) near two ends, rotate between first position sleeve (18) and fourth position sleeve (33) and be provided with first jacking channel-section steel (34), it is provided with second jacking channel-section steel (35) to rotate between second position sleeve (25) and third position sleeve (30).
8. A cell freezing device for monoclonal antibody preparation is characterized in that the freezing device works as follows:
the method comprises the following steps: an output shaft of a first motor (5) drives a screw rod (11) to rotate, the screw rod (11) is matched with an inner screw sleeve (61) to drive an upper lifting plate (6) to ascend, a rod sleeve (62) on the upper lifting plate (6) ascends along an upright rod (12), the upper lifting plate (6) drives a connecting plate (13) to ascend, a first jacking channel steel (34) and a second jacking channel steel (35) rotate to drive a lower lifting plate (26) to ascend, a right-angle fixture block (37) clamps a top surface fixing frame (39) to drive a freezing frame (38) to ascend, the freezing frame (38) ascends from a freezing frame groove (41), a positioning rod (42) ascends from a rod groove (43) to lift the freezing frame (38), and the freezing frame (38) moves out of a freezing cabinet (2);
step two: an output shaft of the second motor (8) drives the gear (9) to rotate, the gear (9) is meshed with the rack (131) to drive the connecting plate (13) to move in the horizontal direction, the sliding block (14) on the connecting plate (13) slides along the sliding rail (10) at the bottom of the ascending and descending plate (6), then the freezer (2) is moved out of the support frame (1), the piston rods of the telescopic cylinders (36) at the two sides of the lower lifting plate (26) push the right-angle clamping block (37), the right-angle clamping block (37) is not clamped with the top surface fixing frame (39), the freezer frame (38) can be taken down, cells are added into the test tube (45) on the freezing rack (38), then the top surface fixing frame (39) on the freezing rack (38) is fixed by the right-angle fixture block (37), the freezing rack (38) enters the freezing cabinet (2), the cabinet door (44) is closed, and the freezing cabinet (2) freezes the cells in the test tube (45) on the freezing rack (38).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010158311.6A CN111296410A (en) | 2020-03-09 | 2020-03-09 | Cell freezing device for monoclonal antibody preparation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010158311.6A CN111296410A (en) | 2020-03-09 | 2020-03-09 | Cell freezing device for monoclonal antibody preparation |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112021303A (en) * | 2020-09-09 | 2020-12-04 | 江苏育瑞康生物科技有限公司 | Cell freezing device and method |
| CN113040132A (en) * | 2021-03-22 | 2021-06-29 | 浙江大学 | Sperm cryopreservation method based on protective agent |
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2020
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112021303A (en) * | 2020-09-09 | 2020-12-04 | 江苏育瑞康生物科技有限公司 | Cell freezing device and method |
| CN113040132A (en) * | 2021-03-22 | 2021-06-29 | 浙江大学 | Sperm cryopreservation method based on protective agent |
| CN113040132B (en) * | 2021-03-22 | 2022-03-25 | 浙江大学 | Sperm cryopreservation method based on protective agent |
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Application publication date: 20200619 |