CN112274582A

CN112274582A - Novel technology for preparing ointment for treating animal wounds

Info

Publication number: CN112274582A
Application number: CN202011211576.4A
Authority: CN
Inventors: 于文会; 姜晓文; 马隽; 汪瑶; 王婕然
Original assignee: Northeast Agricultural University
Current assignee: Northeast Agricultural University
Priority date: 2020-11-03
Filing date: 2020-11-03
Publication date: 2021-01-29

Abstract

The invention discloses a new technology for preparing ointment for treating animal wounds, which comprises the following components: the ointment comprises the following components of lithospermum, frankincense, myrrh, phellodendron, pseudo-ginseng, dragon's blood, borneol, medicinal soybean oil, vaseline, lanolin and white wax, wherein the components are as follows: radix Arnebiae, Olibanum, Myrrha, cortex Phellodendri, radix Notoginseng, sanguis Draxonis, Borneolum Syntheticum, medicinal soybean oil, vaseline, lanolin and white wax; comprises the following steps of firstly, micronizing the traditional Chinese medicine; step two, heating the medicine; step three, stirring uniformly; step four, melting and processing; step five, processing paraffin; step six, bottling and sealing; step seven, sterilization; the invention is energy-saving and environment-friendly, and does not generate waste; the ointment prepared from the traditional Chinese medicine micro powder and the medicinal soybean oil has better effects of stopping bleeding, relieving pain and promoting granulation than the prior preparation technology; the traditional Chinese medicine micro powder and the medicinal soybean oil are used for replacing sesame oil, so that the pharmaceutical cost is reduced, and the clinical popularization and application of veterinarians are facilitated.

Description

Novel technology for preparing ointment for treating animal wounds

Technical Field

The invention relates to the technical field of veterinary medicines, in particular to a novel preparation technology of an ointment for treating animal wounds.

Background

The preparation method of traditional ointment comprises boiling oleum Sesami, frying in oil for a certain time, and removing the traditional Chinese medicine as residue; cooling oleum Sesami to room temperature, filtering, removing impurities, adding vaseline, white wax, and lanolin, and making into ointment. In the new technology, the traditional Chinese medicine superfine powder is used for replacing deep-frying, and the medicinal soybean oil is used for replacing sesame oil. Heating medicinal soybean oil to 80 deg.C, heating the Chinese medicinal micropowder, stirring, and adding adjuvants; in the ointment preparation process, no waste is discharged, and the ointment is energy-saving and environment-friendly.

The traditional ointment pharmaceutical technology has the defects that: the utilization rate of the medicinal components of the traditional Chinese medicinal materials is not high, a part of the active components of the traditional Chinese medicinal materials are still left in the dregs, the clinical curative effect of the ointment is poor, and the dregs serving as wastes need further harmless treatment. During the frying process of the medicinal materials, active ingredients cannot be fully released, the content of the traditional Chinese medicines in the ointment must be increased in order to improve the clinical curative effect, and the added sesame oil is expensive, so the traditional ointment preparation technology has high cost and poor curative effect; in view of these drawbacks, there is a need for a new ointment for treating animal wounds.

Disclosure of Invention

The invention aims to provide a novel technology for preparing ointment for treating animal wounds, which aims to solve the problems in the background technology.

In order to solve the technical problems, the invention provides the following technical scheme: an external ointment for treating animal wound comprises the following components: the ointment comprises the following components of lithospermum, frankincense, myrrh, phellodendron, pseudo-ginseng, dragon's blood, borneol, medicinal soybean oil, vaseline, lanolin and white wax, wherein the components are as follows: lithospermum, frankincense, myrrh, phellodendron, pseudo-ginseng, dragon's blood, borneol, medicinal soybean oil, vaseline, lanolin and white wax.

A method for preparing ointment for treating animal wound comprises the following steps of micronizing Chinese medicinal materials; step two, heating the medicine; step three, stirring uniformly; step four, melting and processing; step five, heating the oil pan; step five, processing paraffin; step six, bottling and sealing; step seven, sterilizing;

in the first step, the superfine grinding of the traditional Chinese medicine comprises the following steps:

1) placing radix Arnebiae, Olibanum, Myrrha, cortex Phellodendri, Notoginseng radix and sanguis Draxonis in oven, and drying;

2) oven drying Chinese medicinal materials, adding radix Arnebiae, Olibanum, Myrrha, cortex Phellodendri, Notoginseng radix and sanguis Draxonis into Chinese medicinal materials superfine pulverizer, and micronizing to obtain micropowder with particle size of above 300 mesh;

in the second step, a medical frying pan is prepared manually, and is cleaned and dried after being sterilized; adding medicinal soybean oil, heating to 80 deg.C, and heating until medicinal soybean oil is melted to obtain refined oil;

in the third step, the traditional Chinese medicine micro powder is poured into a medical frying pan and is continuously stirred until the mixture is uniformly stirred;

in the fourth step, the borneol is manually added into the medical frying pan, and is stirred uniformly after being melted;

adding the melted white wax into a medical frying pan, and fully and uniformly stirring to obtain liquid medicine;

in the sixth step, the Chinese medicine liquid in the fifth step is quickly filled into a specified medicine bottle while the liquid is hot, and the bottle opening is sealed after the liquid is solidified;

wherein in the seventh step, the sealed Co for the Chinese medicine bottle in the sixth step⁶⁰And (5) sterilizing.

According to the technical scheme, a dryer can also be used for drying in the step 1).

According to the technical scheme, heating is carried out for 20-40min in the second step.

According to the technical scheme, the medicinal powder in the third step needs to be sieved and filtered.

According to the technical scheme, the borneol in the fourth step needs to be detected whether impurities exist.

According to the technical scheme, the white wax in the fifth step can be quickly melted by a dryer.

Compared with the prior art, the invention has the following beneficial effects:

1. energy saving and environmental protection, and no waste is generated.

2. The ointment prepared from the traditional Chinese medicine micro powder and the medicinal soybean oil has stronger effects of stopping bleeding, relieving pain and promoting granulation than the prior preparation technology.

3. The traditional Chinese medicine micro powder and the medicinal soybean oil are used for replacing sesame oil, so that the pharmaceutical cost is reduced, and the clinical popularization and application of veterinarians are facilitated.

Drawings

The accompanying drawings, which are included to provide a further understanding of the invention and are incorporated in and constitute a part of this specification, illustrate embodiments of the invention and together with the description serve to explain the principles of the invention and not to limit the invention. In the drawings:

FIG. 1 is a process flow diagram of the present invention;

fig. 2 is a flow chart of a prior art ointment preparation process.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Referring to fig. 1, the present invention provides a technical solution: a new preparation technology of ointment for treating animal wound comprises:

example 1:

an external ointment for treating animal wound comprises the following components: the ointment comprises the following components of lithospermum, frankincense, myrrh, phellodendron, pseudo-ginseng, dragon's blood, borneol, medicinal soybean oil, vaseline, lanolin and white wax, wherein the components are as follows: lithospermum, frankincense, myrrh, phellodendron, pseudo-ginseng, dragon's blood, borneol, medicinal soybean oil, vaseline, lanolin and white wax.

1) placing radix Arnebiae, Olibanum, Myrrha, cortex Phellodendri, Notoginseng radix and sanguis Draxonis in a drying oven, and drying thoroughly, or drying with a dryer;

in the second step, a medical frying pan is prepared manually, and is cleaned and dried after being sterilized; adding medicinal soybean oil, heating to 80 deg.C for 20-40min until medicinal soybean oil is melted to obtain refined oil;

in the third step, the traditional Chinese medicine micro powder is poured into a medical decocting pot, and the medicinal powder is sieved and filtered firstly and is continuously stirred until the medicinal powder is uniformly stirred;

in the fourth step, the borneol is manually added into the medical frying pan, and the borneol is stirred uniformly after the borneol is melted and whether impurities exist or not needs to be detected;

adding the melted white wax into a medical frying pan, quickly melting the white wax by using a dryer, and fully and uniformly stirring to obtain a liquid medicine;

Example 2:

Example 3:

Example 4:

in the properties of each example, 9 healthy Chinese garden dogs are adopted, the weight of each dog is 5-10kg, the average weight of each dog is 6kg, the dogs are aged for 2-4 years, after the dogs are raised for two weeks conventionally, the weight of each dog is weighed and recorded, the dogs are injected subcutaneously with 0.2mL/kg of dog sleeping aid for general anesthesia, the backs of the dogs are shorn from the shoulder blade parts to the buttocks by an electric push, the dogs are degreased and disinfected firstly, then disinfected by iodine tincture, finally deiodinated and disinfected, the dogs are cut off in a full layer by a self-made puncher at the same position on the left and right of the backs of the dogs about 6cm away from the central line of the backs of the dogs according to the sizes of the animals, symmetrical round skin wound surfaces with the diameter of about 150mm are cut, the wound model dogs are randomly divided into a new technical ointment group (X group), a prior technical ointment group (Y group) and a blank control group (K group), 3 dogs are coated with the new technical ointment, each dog is independently raised in a single cage, bound to avoid biting and licking, so as to avoid infection, provide sufficient food and water and keep the living environment suitable; collecting blood from forearm vein of dog, separating serum, detecting indexes such as IL-6, TNF-alpha, P-substance, 5-hydroxytryptamine by ELISA method, photographing wound, calculating wound healing rate, and evaluating clinical effect of ointment prepared by new technology on the day after operation (day 0, day 7, day 14 and day 21);

1) the ointment prepared by the new technology has the following detection results on the wound healing rate of the dog:

measuring the wound area of each group of dogs on 1, 7, 14 and 21 days after operation respectively, then calculating the healing rate of each wound and calculating the average value; the wound healing rate of the dog is increased along with the time; wherein the wound healing rates were significantly increased in both group X and group Y (P <0.05) on days 7 and 14 as compared to group K; wherein, the X group shows extremely remarkable increase (P < 0.01); the results are shown in Table 1.

TABLE 1 Effect of wound-promoting ointment on healing Rate (%)

Note: p <0.05, representing significant differences compared to group K; p <0.01, representing very significant differences compared to group K; Δ P <0.05, representing significance compared to group Y; the following is the same;

2) the ointment prepared by the new technology has the following detection results on the healing time of the canine wound:

as shown in table 2: mean wound healing time was significantly increased in both X and Y groups compared to K group (P < 0.05). Wherein, the X group shows extremely remarkable increase (P < 0.01); on day 21 post-surgery, there was no significant difference between groups compared to group K.

TABLE 2 Effect of wound-promoting and muscle-growing ointment on healing time (d)

3) The ointment prepared by the new technology has the advantages of detecting the scar area after the healing of the canine wound:

as shown in Table 3, the X group and the Y group have very significant difference compared with the K group, which indicates that the effect of the trauma and granulation promoting ointment is equivalent to that of the rubber and granulation promoting ointment, and the area of the prognosis scar can be significantly reduced.

TABLE 3 Effect of wound-promoting ointment on the prognosis of scar area

3) The ointment prepared by the new technology has the following detection result on the anti-inflammatory effect:

peripheral blood leukocyte number detection results: as shown in Table 4, peripheral blood leukocytes increased and decreased first within 1 to 21 days after the wound healed; on the day of trauma, day 0, the number of leukocytes in each group was within the normal range; on day 1 post-injury, white blood cell counts increased, but were also within the normal range; from day 7, the number of the white blood cells in each group is in a clear ascending trend, compared with the K group, the X group and the Y group have obvious difference, and the number change of the white blood cells in the X group and the Y group has no obvious difference; 14 days after the model building, the number of peripheral blood leucocytes begins to be reduced, and compared with a group K, the group X and the group Y are obviously different; the leukocytes recovered to within normal values in each group 21 days after the model creation, but the K group was slower than the X and Y groups, and the number of leukocytes did not change much between the X and Y groups.

TABLE 4 number of peripheral blood leukocytes (× 10) of wound myogenic ointment⁹of/L) of

IL-6 assay results: as shown in Table 5, the IL-6 content showed a tendency to increase and decrease in the whole process; the IL-1 content gradually increases from day 0 to day 1, and decreases from day 7 to day 21; on day 7, the X group and the Y group were significantly different from the K group, and on day 14, the X group and the Y group were significantly different from the K group; on day 21, there was no significant difference between groups.

TABLE 5 ELISA test for IL-6 concentration (pg/mL) in plasma as an index of inflammation

TNF-alpha assay results: from day 0-21, the plasma TNF- α levels first increased and decreased, and there was no significant difference between the X and Y groups; wherein the peak is reached at day seven on days 0-1 and there is no significant difference in the TNF- α levels in the plasma between groups; on the 7 th day after molding, compared with the group K, the group X and the group Y are obviously different; at day 14, there was a very significant difference in group X compared to group K, and a significant difference in group Y; on day 21, there was no statistical significance between groups.

TABLE 6 ELISA test for inflammation index TNF- α (pg/mL)

P-substance detection results: as can be seen from Table 7, the P-substance content showed a tendency to increase and decrease first in days 0 to 21. The X group and the Y group have no significant difference; each group showed an ascending trend 0-1 day after the trauma, and there was no significant difference in each component; a downward trend from day 7-21, with significant differences between groups X and Y compared to group K on day 7; on day 14, the X group was significantly different from the K group, and the Y group was significantly different from the K group; on day 21, there was no statistical significance.

5-hydroxytryptamine detection result: as shown in table 8, the 5-HT content in plasma increased first during the entire time period in a decreasing trend, wherein group X and group Y were significantly different and statistically significant compared to group K at day 7; on day 14, there was a significant difference between the X and K groups, a very significant difference between the Y and K groups, and a significant difference between the X and Y groups.

TABLE 8 ELISA assay for 5-hydroxytryptamine levels (ng/mL) in plasma

4) The ointment prepared by the new technology has the following safety experiment detection results:

the toxicity or side effect test of the ointment is carried out by utilizing Kunming white mice, the ointment has no irritation to the skin, mucous membrane and wound tissues of the white mice, inflammatory reactions such as red, swelling, heat, pain and the like are not seen in the applied tissues, and adverse systemic reactions are not seen in the white mice, so that the traditional Chinese medicine ointment is safe for treating animal wounds and has no toxicity or side effect.

Acute toxicity test: the test group showed no significant difference in body weight compared to the blank control group, but the weight gain of the skin damaged group was reduced, mainly due to traumatic stress, and the results are shown in table 9.

Table 9 weight change before and after each group administration (n ═ 10)

Skin irritation test: the damaged skin and the intact skin have no erythema or edema after 1h, 24h, 48h and 72h after administration, and no erythema or edema is observed after one week after stopping administration, so the antiseptic tissue regeneration promoting ointment is considered as a medicament without obvious skin irritation, and the results are shown in Table 10.

Table 10 skin irritation test results for each group (n ═ 10)

Skin allergy test:

the results of the sensitization reaction score and sensitization rate test comparison between the tissue regeneration promoting paste and matrix and the positive drug group show that the tissue regeneration promoting paste and the matrix have no skin sensitization, and are shown in tables 11 and 12.

Table 11 results of allergic reaction test (n ═ 10)

TABLE 12 sensitization rate test results for each group (n ═ 10)

Based on the above, the invention has the advantages of energy saving, environmental protection and no waste generation; the ointment prepared from the traditional Chinese medicine micro powder and the medicinal soybean oil has better effects of stopping bleeding, relieving pain and promoting granulation than the prior preparation technology; the traditional Chinese medicine micro powder and the medicinal soybean oil are used for replacing sesame oil, so that the pharmaceutical cost is reduced, and the clinical popularization and application of veterinarians are facilitated.

It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus.

Finally, it should be noted that: although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that changes may be made in the embodiments and/or equivalents thereof without departing from the spirit and scope of the invention. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. An external ointment for treating animal wounds is characterized in that: the formula comprises the following components: the ointment comprises the following components of lithospermum, frankincense, myrrh, phellodendron, pseudo-ginseng, dragon's blood, borneol, medicinal soybean oil, vaseline, lanolin and white wax, wherein the components are as follows: lithospermum, frankincense, myrrh, phellodendron, pseudo-ginseng, dragon's blood, borneol, medicinal soybean oil, vaseline, lanolin and white wax.

2. A method for preparing ointment for treating animal wound comprises the following steps of micronizing Chinese medicinal materials; step two, heating the medicine; step three, stirring uniformly; step four, melting and processing; step five, processing paraffin; step six, bottling and sealing; step seven, sterilizing; the method is characterized in that:

3. A method of preparing an ointment for treating animal wounds according to claim 2, comprising: the step 1) can also be dried by a dryer.

4. A method of preparing an ointment for treating animal wounds according to claim 2, comprising: and heating for 20-40min in the second step.

5. A method of preparing an ointment for treating animal wounds according to claim 2, comprising: the medicinal powder in the third step needs to be sieved and filtered.

6. A method of preparing an ointment for treating animal wounds according to claim 2, comprising: and in the fourth step, the borneol needs to be detected whether impurities exist or not.

7. A method of preparing an ointment for treating animal wounds according to claim 2, comprising: and in the fifth step, the white wax can be quickly melted by a dryer.