CN112255207A - Fluorescence excitation acquisition system - Google Patents
Fluorescence excitation acquisition system Download PDFInfo
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- CN112255207A CN112255207A CN202011001770.XA CN202011001770A CN112255207A CN 112255207 A CN112255207 A CN 112255207A CN 202011001770 A CN202011001770 A CN 202011001770A CN 112255207 A CN112255207 A CN 112255207A
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- 230000005284 excitation Effects 0.000 title claims abstract description 36
- 230000003287 optical effect Effects 0.000 claims abstract description 47
- 238000000926 separation method Methods 0.000 claims abstract description 35
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- 239000012491 analyte Substances 0.000 claims abstract description 7
- 238000007789 sealing Methods 0.000 claims description 4
- 238000003780 insertion Methods 0.000 claims description 2
- 230000037431 insertion Effects 0.000 claims description 2
- 238000000034 method Methods 0.000 claims 1
- 230000005855 radiation Effects 0.000 claims 1
- 238000001917 fluorescence detection Methods 0.000 abstract description 4
- 238000004458 analytical method Methods 0.000 description 13
- 238000001514 detection method Methods 0.000 description 5
- 238000005286 illumination Methods 0.000 description 4
- 238000006243 chemical reaction Methods 0.000 description 3
- 230000017525 heat dissipation Effects 0.000 description 3
- 230000005281 excited state Effects 0.000 description 2
- 238000009434 installation Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 238000006467 substitution reaction Methods 0.000 description 2
- 238000003556 assay Methods 0.000 description 1
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- G—PHYSICS
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6408—Fluorescence; Phosphorescence with measurement of decay time, time resolved fluorescence
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N2021/6463—Optics
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N2021/6463—Optics
- G01N2021/6471—Special filters, filter wheel
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Abstract
The invention relates to the technical field of a time-resolved fluorescence acquisition system of biotechnology, and discloses a fluorescence excitation acquisition system, which comprises a photon collector, a fluorescence filtering module and a light source module; the fluorescence filtering module is respectively connected with the photon collector and the light source module; the fluorescence filtering module comprises a lens module shell, a first optical filter, a condensing convex lens, a condensing sheet, a color separation sheet component, a dustproof cover plate, a lens convex lens, a second optical filter, a lens concave seat and a lens convex seat; a light source generated by the light source module enters the analyte solution through a path formed by the first optical filter, the condensing convex lens, the condensing sheet, the color separation sheet assembly and the dustproof cover plate to be excited to generate fluorescence; the fluorescence enters the photon collector through a path formed by the dustproof cover plate, the color separation plate assembly, the lens convex lens, the second optical filter, the lens concave seat and the lens convex seat. The fluorescence excitation acquisition system provided by the invention has the advantages of small volume, low cost and simple operation, and simultaneously meets the requirement of clinical rapid fluorescence detection.
Description
Technical Field
The invention relates to the technical field of time-resolved fluorescence acquisition systems of biotechnology, in particular to a fluorescence excitation acquisition system for acquiring fluorescence signals by exciting an analyte solution to generate fluorescence with a specific waveband.
Background
Certain analyte solutions emit light at a longer wavelength than the excitation light upon exposure to light, and the substance is capable of absorbing and storing energy (e.g., light energy, chemical energy, etc.) from outside and entering an excited state; when it returns from the excited state to the ground state, excess energy is emitted as electromagnetic radiation (i.e., luminescence), referred to as fluorescence. Two conditions must be met for fluorescence to occur: the molecules in the analyte solution must have the same frequency as the illuminating light; molecules that absorb energy at the same frequency as their characteristic frequencies must have high fluorescence efficiency. The requirement for clinical detection of fluorescence is increasingly large in the market, but the traditional fluorescence acquisition system is generally large in size, complex to operate and high in price, and the generated stray light of the light source has a large influence on the fluorescence acquisition of the photon collector, so that the requirement for clinical rapid detection is difficult to meet.
Disclosure of Invention
The invention aims to overcome the problems in the prior art and provide a fluorescence excitation acquisition system which has small volume, low cost and simple operation and simultaneously meets the requirement of clinical rapid fluorescence detection.
In order to achieve the purpose, the invention adopts the following scheme:
a fluorescence excitation acquisition system comprises a photon collector; the device also comprises a fluorescence filtering module and a light source module; the fluorescence filtering module is respectively connected with the photon collector and the light source module;
the fluorescence filtering module comprises a lens module shell, a first optical filter, a condensing convex lens, a condensing sheet, a color separation sheet assembly, a dustproof cover plate, a lens convex lens, a second optical filter, a lens concave seat and a lens convex seat;
a light source generated by the light source module enters the analyte solution through a path formed by the first optical filter, the light-gathering convex lens, the light-gathering sheet, the color separation sheet assembly and the dustproof cover plate to be excited to generate fluorescence;
and the fluorescence enters the photon collector through a path formed by the dustproof cover plate, the color separation plate assembly, the lens convex lens, the second optical filter, the lens concave seat and the lens convex seat.
Furthermore, the photon collector is arranged on the upper end of the fluorescence filtering module; the light source module is arranged at the left end of the fluorescence filtering module;
a first lens hole cavity is formed in the upper direction and the lower direction of the lens module shell; a second lens hole cavity communicated with the first lens hole cavity is formed in the left and right direction of the lens module shell;
the optical filter, the condensing convex lens and the condensing sheet are sequentially arranged in the first lens hole cavity from left to right; the centers of the optical filter, the condensing convex lens and the condensing sheet are aligned with the central axis of the first lens hole cavity;
the lens convex lens, the optical filter, the lens concave seat and the lens convex seat are sequentially arranged at the upper end of the lens module shell from bottom to top; the centers of the lens convex mirror, the optical filter, the lens concave seat and the lens convex seat are aligned with the central axis of the second lens cavity; the photon collector is arranged on the lens convex seat;
the color separation sheet component is obliquely arranged at the junction of the first lens hole cavity and the second lens hole cavity to refract light into the second lens hole cavity;
the dustproof cover plate is arranged at the lower end of the second lens hole cavity and is aligned with the central axis of the second lens hole cavity.
Furthermore, the first optical filter, the light-gathering convex lens and the light-gathering sheet are arranged in the first lens hole cavity through the lens sleeve gasket assembly; the left end of the first lens hole cavity is provided with a positioning clamping groove which is matched and connected with the outer wall of the lens sleeve gasket component;
the lens sleeve gasket assembly comprises a lens sleeve gasket, a first sleeve locking joint and a second sleeve locking joint; the lens cushion sleeve, the first sleeve locking joint and the second sleeve locking joint are uniformly communicated with the first lens cavity;
the first optical filter and the light-gathering convex mirror are sequentially arranged in the first sleeve locking joint from left to right; the first sleeve locking joint is connected with the left end of the lens cushion sleeve to position the first optical filter and the light-gathering convex lens; the first sleeve locking joint is connected with the light source module; a sealing ring is arranged between the light-gathering convex lens and the left end of the lens cushion sleeve;
the light-gathering sheet is arranged in the right end of the lens cushion sleeve; the second sleeve locking joint is connected with the right end of the lens cushion sleeve to position the light-gathering sheet.
Furthermore, the inner surface of the right end of the first sleeve locking joint is provided with a first internal thread; the outer surface of the left end of the lens cushion sleeve is provided with a first external thread which is matched and connected with the first internal thread; the outer surface of the second sleeve locking joint is provided with second external threads; and a second internal thread which is matched and connected with the second external thread is arranged on the inner surface of the right end of the lens cushion sleeve.
Further, the color separation plate assembly forms an included angle of 45 degrees with the horizontal plane in an inclined mode.
Furthermore, a positioning slot for positioning the color separation sheet assembly is arranged at the junction of the first lens hole cavity and the second lens hole cavity on the lens module shell; a positioning cover groove and a positioning cover plate are arranged at the rear end of the lens module shell corresponding to the positioning insertion groove; the positioning cover plate is embedded in the positioning cover groove in a matched mode and is locked and fixed through bolts.
Furthermore, a positioning groove is arranged at the position, corresponding to the second lens hole cavity, on the upper end of the lens module shell; the lens convex mirror and the optical filter are arranged on the positioning groove in a matched manner from top to bottom; the lens recess is arranged on the upper end surface of the lens module shell; the lens convex seat is arranged on the upper end surface of the lens concave seat.
Furthermore, the light source module comprises a point light source and a light source fixing seat; the point light source is arranged on the lamp source fixing seat; the first sleeve locking joint is connected with the lamp source fixing seat; the position of the light source fixing seat corresponding to the first lens hole cavity is provided with an opening; the lamp source fixing seat is detachably connected to the lens module shell through a screw.
Further, the lens module shell comprises a first block module shell and a second block module shell; the first block module shell is connected with the second block module shell; the second lens hole cavity is arranged on the second square module shell; the first lens hole cavity penetrates through the first block module shell and penetrates through the second block module shell to be communicated with the second lens hole cavity.
Furthermore, a heat dissipation fan connected with the lens module shell is arranged at the front end of the light source module.
Compared with the prior art, the invention has the following advantages:
the invention utilizes the light source module to emit the light source with specific wavelength, the light path enters the analysis solution through setting, the analysis solution excites to generate fluorescence, then the fluorescence generated by the analysis solution after the photon collector is used for collecting excitation is used for signal intensity, the concentration of the solution is fed back by processing feedback, the problem of low illumination excitation efficiency is solved, the influence of stray light of the light source on the fluorescence collected by the photon collector is large, the problem of unstable fluorescence signal collected at low concentration is solved, and the volume of the fluorescence excitation collection system is small, the cost is low, the operation is simple, and the requirement of clinical rapid fluorescence detection is met.
Drawings
The present application will be described in further detail with reference to the following drawings and detailed description.
FIG. 1 is a perspective view of a fluorescence excitation acquisition system of the present invention.
Fig. 2 is an exploded perspective view of the fluorescence excitation collection system of the present invention.
Figure 3 is a cross-sectional view of a fluorescence excitation acquisition system of the present invention.
The figure includes: .
The photon collector 1, the fluorescence filtering module 2, the lens module housing 20, the first module housing 201, the second module housing 202, the first lens cavity 2011, the second lens cavity 2021, the first optical filter 21, the condensing convex lens 22, the condensing sheet 23, the color separation sheet assembly 24, the color separation sheet 241, the color separation sheet seat 242, the dustproof cover sheet 25, the lens convex lens 26, the second optical filter 27, the lens recess 28, the lens convex seat 29, the light source module 3, the point light source 31, the light source fixing seat 32, the lens cushion assembly 4, the lens cushion 41, the first sleeve locking joint 42, the second sleeve locking joint 43, the positioning slot 5, the sealing ring 6, the first internal thread 421, the first external thread 411, the second internal thread 412, the second external thread 431, the positioning slot 7, the positioning cover slot 8, the positioning cover plate 9, the positioning slot 10, and the cooling fan 11.
Detailed Description
The following detailed description of embodiments of the present invention is provided in connection with the accompanying drawings and examples. The following examples are intended to illustrate the invention but are not intended to limit the scope of the invention.
As shown in fig. 1 to 3, a fluorescence excitation collection system includes a photon collector 1, a fluorescence filter module 2 and a light source module 3; the photon collector 1 comprises a reading value collecting sensor, an XH-T terminal, a plug and a fixed wire workpiece. The fluorescence filtering module 2 is respectively connected with the photon collector 1 and the light source module 3; the fluorescence filter module 2 comprises a lens module shell 20, a first optical filter 21, a condensing convex lens 22, a condensing sheet 23, a color separation sheet assembly 24, a dustproof cover plate 25, a lens convex lens 26, a second optical filter 27, a lens concave seat 28 and a lens convex seat 29; a light source generated by the light source module 3 enters an analyte solution through a path formed by the first optical filter 21, the light-gathering convex lens 22, the light-gathering sheet 23, the color separation sheet assembly 24 and the dustproof cover plate 25 to be excited to generate fluorescence; fluorescence enters the photon collector 1 through a path formed by the dustproof cover plate 25, the color separation plate assembly 24, the lens convex lens 26, the second optical filter 27, the lens concave seat 28 and the lens convex seat 29. The fluorescence excitation collection system has a small overall structure volume by concentrating the structures such as the first optical filter 21, the light-gathering convex lens 22, the light-gathering sheet 23, the color separation sheet assembly 24, the dustproof cover plate 25, the lens convex lens 26, the second optical filter 27, the lens concave seat 28, the lens convex seat 29 and the like on the lens module shell 20. The light source module 3 emits light with a specific wavelength, the light is filtered by the first optical filter 21, the size of a light spot is controlled by the light-condensing convex lens 22 and the light-condensing sheet 23, and then the light enters an analysis solution of the reaction vessel for fluorescence excitation through refraction of the light-condensing sheet assembly 24 and the dustproof cover plate 25; the analysis solution generates fluorescence, the fluorescence reaches the lens convex lens 26 through the dustproof cover plate 25 and the color separation plate assembly 24, the fluorescence exit angle is controlled, then the fluorescence enters the second optical filter 27 for fluorescence filtration, finally the fluorescence enters the photon collector 1 through the lens concave seat 28 and the lens convex seat 29, and the fluorescence signal is collected by the photon collector 1.
This fluorescence arouses collection system utilizes light source module 3 to send the light source of specific wavelength, light path through setting for gets into analysis solution, analysis solution arouses and produces fluorescence, then utilize photon collector 1 to gather the fluorescence that analysis solution after arousing produced and carry out the power of signal, the concentration of handling the feedback this solution, it is not high to solve illumination excitation efficiency, the light source miscellaneous light is to the great problem of photon collector 1 collection fluorescence influence, and solve the unstable problem of low concentration collection fluorescence signal, and this fluorescence arouses collection system's small, with low costs, the operation is thus simple, satisfy the demand of clinical short-term detection fluorescence simultaneously.
Specifically, the photon collector 1 is arranged on the upper end of the fluorescence filtering module 2; the light source module 3 is arranged at the left end of the fluorescence filtering module 2; the lens module housing 20 has a first lens cavity 2011 in the vertical direction; the lens module housing 20 has a second lens cavity 2021 communicating with the first lens cavity 2011 in the left-right direction; that is, the first lens cavity 2011 is perpendicular to the second lens cavity 2021; the optical filter, the condensing convex lens 22 and the condensing sheet 23 are sequentially arranged in the first lens cavity 2011 from left to right; the centers of the optical filter, the condensing convex lens 22 and the condensing sheet 23 are aligned with the central axis of the first lens cavity 2011; the lens convex lens 26, the optical filter, the lens concave seat 28 and the lens convex seat 29 are sequentially arranged at the upper end of the lens module shell 20 from bottom to top; the centers of the lens convex mirror 26, the optical filter, the lens concave seat 28 and the lens convex seat 29 are aligned with the central axis of the second lens cavity 2021; the photon collector 1 is arranged on the lens convex base 29; the color separation sheet assembly 24 is obliquely arranged at the junction of the first lens cavity 2011 and the second lens cavity 2021 to refract light into the second lens cavity 2021; the dust-proof cover plate 25 is arranged on the lower end of the second lens hole cavity 2021 and is aligned with the central axis of the second lens hole cavity 2021. When the light source module 3 emits light with a specific wavelength, the light enters from the left end of the lens module housing 20, is filtered by the first optical filter 21, passes through the condensing convex lens 22 and the condensing sheet 23 to control the size of light spots, passes through the dichroic filter assembly 24, and is refracted by the dichroic filter assembly 24 through the dustproof cover plate 25; the light then enters the assay solution in the reaction vessel for fluorescence excitation. The analysis solution produces fluorescence, the fluorescence passes through dustproof cover plate 25 again straight line, color separation piece subassembly 24 reaches lens convex mirror 26, control fluorescence outgoing angle, then get into second light filter 27 and carry out fluorescence and filter, finally pass through lens concave seat 28, lens convex seat 29 gets into photon collector 1, carry out the collection of fluorescence signal by photon collector 1, it is not high to solve illumination excitation efficiency effectively, the light source miscellaneous light is to the great problem of photon collector 1 collection fluorescence influence, and solve and gather the unstable problem of fluorescence signal at low concentration, and this fluorescence excitation collection system's is small, low cost, and easy operation, satisfy clinical rapid detection fluorescence's demand simultaneously.
In order to ensure the accuracy of the refraction angle of the color separation plate assembly 24, the included angle formed by the color separation plate assembly 24 and the horizontal plane is 45 degrees, and the positive and negative error values are 0.5 degree. Through setting up color separation piece subassembly 24 and horizontal plane slope and forming 45 degrees contained angles, when light when passing through color separation piece subassembly 24, color separation piece subassembly 24 can refract 90 degrees with the light, carries out fluorescence excitation in the analysis solution that gets into reaction vessel through dustproof cover 25. The color separator assembly 24 includes a color separator 241 and a color separator holder 242, and the color separator 241 is precisely positioned by the color separator holder 242.
The lens module housing 20 includes a first block module housing 201 and a second block module housing 202, and is a square structure; the first block module housing 201 is connected to the second block module housing 202; the second lens cavity 2021 is disposed on the second block module housing 202; the first lens bore 2011 penetrates the first square module case 201 and penetrates the second square module case 202 to communicate with the second lens bore 2021. Through setting up first square module shell 201 and second square module shell 202, the installation of first light filter 21, spotlight convex lens 22, condensing lens 23, color separation piece subassembly 24, dustproof cover plate 25, lens convex lens 26, second light filter 27, camera lens concave seat 28 and camera lens convex seat 29 are convenient for, have sufficient space, simultaneously, make this fluorescence arouse collection system whole pleasing to the eye. Of course, the lens module housing 20 may also take other shapes, such as a cylinder.
In this embodiment, the first optical filter 21, the light-gathering convex lens 22, and the light-gathering sheet 23 are disposed in the first lens cavity 2011 through the lens sleeve pad assembly 4; the left end of the first lens cavity 2011 is provided with a positioning clamping groove 5 which is matched and connected with the outer wall of the lens sleeve gasket component 4; the lens sleeve assembly 4 comprises a lens sleeve 41, a first sleeve locking joint 42 and a second sleeve locking joint 43; the lens pad sleeve 41, the first sleeve locking joint 42 and the second sleeve locking joint 43 are uniformly communicated with the first lens cavity 2011; the first optical filter 21 and the light-gathering convex lens 22 are sequentially arranged in the first sleeve locking joint 42 from left to right; the first sleeve locking joint 42 is connected with the left end of the lens cushion sleeve 41 to position the first optical filter 21 and the light-gathering convex lens 22; the first sleeve locking joint 42 is connected with the light source module 3; a sealing ring 6 is arranged between the light-gathering convex lens 22 and the left end of the lens cushion sleeve 41; the light-gathering sheet 23 is arranged in the right end of the lens cushion sleeve 41; the second sleeve locking connector 43 is connected to the right end of the lens pad sleeve 41 to position the light collection sheet 23. This lens sleeve pad assembly 4 is made of soft materials such as rubber, and is conveniently installed in first lens cavity 2011. Form lens sleeve gasket subassembly 4 through lens sleeve gasket 41, first sleeve pipe lock joint 42 and second sleeve pipe lock joint 43, conveniently concentrate first light filter 21, spotlight convex lens 22, condensing sheet 23 on lens sleeve gasket subassembly 4, simple to operate, accurate location avoids appearing detection error, helps satisfying the demand of clinical short-term test fluorescence.
In this embodiment, the inner surface of the right end of the first sleeve locking joint 42 is provided with a first internal thread 421; the outer surface of the left end of the lens pad sleeve 41 is provided with a first external thread 411 which is matched and connected with the first internal thread 421; the outer surface of the second sleeve locking sub 43 is provided with a second external thread 431; the inner surface of the right end of the lens pad sleeve 41 is provided with a second internal thread 412 which is in fit connection with the second external thread 431. Through setting up first internal thread 421, first external screw thread 411, second internal thread 412, have second external screw thread 431, realize that first sleeve lock joint 42, second sleeve lock joint 43 can dismantle with lens pad sleeve 41 respectively and be connected simple structure, simple to operate. Of course, in some embodiments, the first and second ferrule locking connectors 42 and 43 may be detachably connected to the lens cushion 41 by a snap-fit connection.
A positioning slot 7 for positioning the color separation sheet assembly 24 is arranged at the junction of the first lens cavity 2011 and the second lens cavity 2021 on the lens module shell 20; a positioning cover groove 8 and a positioning cover plate 9 are arranged at the rear end of the lens module shell 20 corresponding to the positioning slot 7; the positioning cover plate 9 is embedded in the positioning cover groove 8 in a matching manner and is locked and fixed through bolts. Through setting up positioning slot 7, make things convenient for color separation piece subassembly 24 to fix a position on lens module shell 20, realize color separation piece subassembly 24's accurate location, simple structure. And he can realize the rear end positioning of the color separation sheet component 24 by arranging the positioning cover plate 9 in the positioning cover groove 8, and simultaneously, the fluorescence excitation acquisition system is integrally beautiful.
A positioning groove 10 is arranged at the position, corresponding to the second lens hole cavity 2021, on the upper end of the lens module shell 20; the lens convex mirror 26 and the optical filter are arranged on the positioning groove 10 in a matching way from top to bottom; through establishing lens convex lens 26, light filter in the constant head tank 10 from the top down in proper order, simple structure, it is fixed convenient with the location, avoids too much location structure's setting and installation. The lens recess 28 is disposed on the upper end surface of the lens module housing 20; the lens boss 29 is provided on the upper end surface of the lens recess 28.
The light source module 3 comprises a point light source 31 and a light source fixing seat 32; the point light source 31 is arranged on the lamp source fixing seat 32; the first sleeve locking joint 42 is connected with the lamp source fixing seat 32; the position of the light source fixing seat 32 corresponding to the first lens hole 2011 is provided with an opening; the light source fixing base 32 is detachably connected to the lens module housing 20 through a screw. Fix point light source 31 through lamp source fixing base 32, simple structure improves point light source 31's stability.
In order to accelerate the heat dissipation of the light source module 3, a heat dissipation fan 11 connected to the lens module housing 20 is disposed at the front end of the light source module 3.
To sum up, the invention provides a fluorescence excitation collection system, which utilizes a light source module 3 to emit a light source with a specific wavelength, the light source enters an analysis solution through a set light path, the analysis solution is excited to generate fluorescence, then a photon collector 1 is utilized to collect the fluorescence generated by the excited analysis solution to perform signal intensity, the concentration of the solution is fed back by processing and feedback, the problems that the illumination excitation efficiency is not high, the influence of stray light of the light source on the collection of the fluorescence by the photon collector 1 is large, and the problem that the collection of the fluorescence signal at low concentration is unstable are solved, and the fluorescence excitation collection system has small volume, low cost and simple operation, and simultaneously meets the requirement of clinical rapid fluorescence detection.
The foregoing is only a preferred embodiment of the present application, and it should be noted that, for those skilled in the art, several modifications and substitutions can be made without departing from the technical principle of the present application, and these modifications and substitutions should also be regarded as the protection scope of the present application.
Claims (10)
1. A fluorescence excitation acquisition system comprises a photon collector; the method is characterized in that: the device also comprises a fluorescence filtering module and a light source module; the fluorescence filtering module is respectively connected with the photon collector and the light source module;
the fluorescence filtering module comprises a lens module shell, a first optical filter, a condensing convex lens, a condensing sheet, a color separation sheet assembly, a dustproof cover plate, a lens convex lens, a second optical filter, a lens concave seat and a lens convex seat;
a light source generated by the light source module enters the analyte solution through a path formed by the first optical filter, the light-gathering convex lens, the light-gathering sheet, the color separation sheet assembly and the dustproof cover plate to be excited to generate fluorescence;
and the fluorescence enters the photon collector through a path formed by the dustproof cover plate, the color separation plate assembly, the lens convex lens, the second optical filter, the lens concave seat and the lens convex seat.
2. The fluorescence excitation acquisition system according to claim 1, wherein: the photon collector is arranged on the upper end of the fluorescence filtering module; the light source module is arranged at the left end of the fluorescence filtering module;
a first lens hole cavity is formed in the upper direction and the lower direction of the lens module shell; a second lens hole cavity communicated with the first lens hole cavity is formed in the left and right direction of the lens module shell;
the optical filter, the condensing convex lens and the condensing sheet are sequentially arranged in the first lens hole cavity from left to right; the centers of the optical filter, the condensing convex lens and the condensing sheet are aligned with the central axis of the first lens hole cavity;
the lens convex lens, the optical filter, the lens concave seat and the lens convex seat are sequentially arranged at the upper end of the lens module shell from bottom to top; the centers of the lens convex mirror, the optical filter, the lens concave seat and the lens convex seat are aligned with the central axis of the second lens cavity; the photon collector is arranged on the lens convex seat;
the color separation sheet component is obliquely arranged at the junction of the first lens hole cavity and the second lens hole cavity to refract light into the second lens hole cavity;
the dustproof cover plate is arranged at the lower end of the second lens hole cavity and is aligned with the central axis of the second lens hole cavity.
3. The fluorescence excitation acquisition system according to claim 2, wherein: the first optical filter, the light-gathering convex lens and the light-gathering sheet are arranged in the first lens hole cavity through the lens sleeve gasket assembly; the left end of the first lens hole cavity is provided with a positioning clamping groove which is matched and connected with the outer wall of the lens sleeve gasket component;
the lens sleeve gasket assembly comprises a lens sleeve gasket, a first sleeve locking joint and a second sleeve locking joint; the lens cushion sleeve, the first sleeve locking joint and the second sleeve locking joint are uniformly communicated with the first lens cavity;
the first optical filter and the light-gathering convex mirror are sequentially arranged in the first sleeve locking joint from left to right; the first sleeve locking joint is connected with the left end of the lens cushion sleeve to position the first optical filter and the light-gathering convex lens; the first sleeve locking joint is connected with the light source module; a sealing ring is arranged between the light-gathering convex lens and the left end of the lens cushion sleeve;
the light-gathering sheet is arranged in the right end of the lens cushion sleeve; the second sleeve locking joint is connected with the right end of the lens cushion sleeve to position the light-gathering sheet.
4. The fluorescence excitation acquisition system according to claim 3, wherein: the inner surface of the right end of the first sleeve locking joint is provided with a first internal thread; the outer surface of the left end of the lens cushion sleeve is provided with a first external thread which is matched and connected with the first internal thread; the outer surface of the second sleeve locking joint is provided with second external threads; and a second internal thread which is matched and connected with the second external thread is arranged on the inner surface of the right end of the lens cushion sleeve.
5. The fluorescence excitation acquisition system according to claim 2, wherein: the color separation sheet assembly and the horizontal plane are inclined to form an included angle of 45 degrees.
6. The fluorescence excitation acquisition system according to claim 2, wherein: a positioning slot for positioning the color separation sheet assembly is arranged at the junction of the first lens hole cavity and the second lens hole cavity on the lens module shell; a positioning cover groove and a positioning cover plate are arranged at the rear end of the lens module shell corresponding to the positioning insertion groove; the positioning cover plate is embedded in the positioning cover groove in a matched mode and is locked and fixed through bolts.
7. The fluorescence excitation acquisition system according to claim 2, wherein: a positioning groove is formed in the upper end of the lens module shell corresponding to the position of the second lens hole cavity; the lens convex mirror and the optical filter are arranged on the positioning groove in a matched manner from top to bottom; the lens recess is arranged on the upper end surface of the lens module shell; the lens convex seat is arranged on the upper end surface of the lens concave seat.
8. The fluorescence excitation acquisition system according to claim 4, wherein: the light source module comprises a point light source and a light source fixing seat; the point light source is arranged on the lamp source fixing seat; the first sleeve locking joint is connected with the lamp source fixing seat; the position of the light source fixing seat corresponding to the first lens hole cavity is provided with an opening; the lamp source fixing seat is detachably connected to the lens module shell through a screw.
9. The fluorescence excitation acquisition system according to claim 2, wherein: the lens module shell comprises a first square module shell and a second square module shell; the first block module shell is connected with the second block module shell; the second lens hole cavity is arranged on the second square module shell; the first lens hole cavity penetrates through the first block module shell and penetrates through the second block module shell to be communicated with the second lens hole cavity.
10. The fluorescence excitation acquisition system according to claim 1, wherein: and the front end of the light source module is provided with a heat radiation fan connected with the lens module shell.
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