CN209764807U - Light source assembly, fluorescence detector and liquid chromatography system - Google Patents

Abstract

The utility model provides a light source subassembly, fluorescence detector and liquid chromatography system belongs to separation technical field, and the light source subassembly includes monochromatic light source, bed frame and first light filter, and monochromatic light source and first light filter are all installed on the bed frame, and first light filter is located the light propagation path of monochromatic light source. The light source assembly is compact in structure, convenient to install and detach, time-saving, labor-saving and high in efficiency. The fluorescence detector includes a light source assembly. Liquid chromatography systems include a light source assembly or a fluorescence detector.

Description

Light source assembly, fluorescence detector and liquid chromatography system

Technical Field

The utility model relates to a separation technology field particularly, relates to a light source subassembly, fluorescence detector and liquid chromatography system.

Background

Liquid chromatography is widely used for separation and analysis of mixed substances as a high-performance separation technology, and a fluorescence detector is one of key components of a liquid chromatography system. Fluorescence analysis is an important method for qualitatively or quantitatively analyzing the characteristics of substances, and has the advantages of high sensitivity, stable signal and the like.

The inventors have found in their studies that the existing fluorescence detectors have at least the following disadvantages:

The existing fluorescence detector is an instrument commonly configured in the existing liquid chromatography system, and has the defects of high lamp source replacement cost and the like.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to provide a light source subassembly, simple structure, it is with low costs, be convenient for change and installation.

The utility model also aims to provide a fluorescence detector, simple structure, it is with low costs, be convenient for change and installation.

An object of the utility model is to provide a liquid chromatography system, simple structure, it is with low costs, be convenient for change and installation.

The utility model discloses an adopt following technical scheme to realize:

Based on above-mentioned first purpose, the embodiment of the utility model provides a light source subassembly, the light source subassembly includes monochromatic light source, bed frame and first light filter, monochromatic light source and first light filter all install in on the bed frame, just first light filter is located on the light propagation path of monochromatic light source.

Optionally, the base frame includes a substrate and a lens barrel support connected to each other, the monochromatic light source is installed on the substrate, the lens barrel support has a light path, and the first optical filter is installed in the light path.

Optionally, the base frame further includes a buffer cover for preventing the first optical filter from falling off from the port of the substrate away from the optical path channel, the buffer cover has an optical path through hole, the buffer cover is mounted on the lens barrel support, and the optical path channel is communicated with the optical path through hole.

Optionally, an abutting portion is provided on an inner wall of the optical path, a gap is provided between the abutting portion and an end of the optical path far from the substrate, and the first optical filter abuts against the abutting portion and the buffer cover at the same time.

Based on above-mentioned second purpose, the embodiment of the utility model provides a fluorescence detector, including the install bin that has the closed cavity and the light source subassembly, the bed frame install in on the install bin, monochromatic light source and first light filter is located in the closed cavity.

Optionally, the fluorescence detector further includes a flow cell, a second optical filter and a photoelectric conversion module, the flow cell and the second optical filter are both installed in the closed chamber, the flow cell is located on a light propagation path of the monochromatic light source, the second optical filter is located on a light propagation path of the fluorescence emitted from the flow cell, and an included angle is formed between a light propagation direction of the monochromatic light source and a light propagation direction of the fluorescence emitted to the second optical filter; the photoelectric conversion assembly is arranged on the installation box and used for receiving the fluorescence which passes through the second optical filter.

Optionally, the fluorescence detector still includes the installation piece, the installation piece install in the closed cavity, be provided with first mounting hole and second mounting hole on the installation piece, first mounting hole with second mounting hole intercommunication just has the contained angle, the flow-through cell install in the first mounting hole, the monochromatic light source inserts in the second mounting hole.

Optionally, the fluorescence detector further includes a fixing member, the second optical filter is mounted on the fixing member, and the fixing member is detachably connected to the mounting box.

Optionally, the fixing member includes a fixing plate and a fixing strip connected to each other, the fixing plate is provided with an installation through hole, and the second optical filter is installed in the installation through hole; the mounting box is provided with an inserting hole communicated with the closed cavity, the fixing plate is in sliding fit with the inserting hole, and the fixing strip is detachably connected with the mounting box.

In accordance with a third aspect of the present invention, there is provided a liquid chromatography system, comprising the light source assembly or the fluorescence detector.

Compared with the prior art, the utility model discloses a beneficial effect of the light source subassembly that preferred embodiment provided includes:

The utility model provides a light source subassembly, monochromatic light source and first light filter are all installed on the bed frame, and monochromatic light source and first light filter are all fixed a position through the bed frame, and the position of the two is more accurate, and light source subassembly during operation reliability is high. Meanwhile, the light source assembly is installed and replaced as an integral structure, the step of debugging the relative position of the monochromatic light source and the first light filter when the light source assembly is replaced is reduced, the installation process is saved, time and labor are saved, the installation and replacement operation is convenient, the installation efficiency and the replacement efficiency of the light source assembly are improved, the structure of the light source assembly is compact, the size is small, the occupied space is small, and the transportation and the storage are convenient.

Furthermore, in the embodiment of the present invention, the base frame includes a base plate and a lens barrel support, the lens barrel support is mounted on the base plate, the lens barrel support has an optical path, the first optical filter is mounted in the optical path, the monochromatic light source is inserted into the optical path, the monochromatic light source and the first optical filter are convenient to mount, and the two are positioned by the optical path, the position accuracy of the two is high, and the mounting accuracy is high.

Furthermore, in the embodiment of the utility model, the bed frame still includes the buffering lid, installs first light filter in light path passageway after, will cushion the lid and establish on the lens cone support, cushions the lid and is used for preventing that first light filter from droing from light path passageway, overall structure safe and reliable more. Simultaneously, the buffering lid has certain cushioning effect, and when the install bin cooperation of light source subassembly and fluorescence detector, the buffering lid butt is on the install bin, and the buffering lid has certain deformability, realizes the elasticity butt, avoids the rigidity butt to cause the damage of spare part.

The embodiment of the utility model provides a fluorescence detector includes foretell light source subassembly, has all advantages of above-mentioned light source subassembly.

furthermore, in an embodiment of the present invention, the second optical filter is mounted on a fixing member, and the fixing member is detachably mounted on the mounting box. When the second optical filter needs to be replaced, the fixing piece is directly detached from the installation box, then the fixing piece different from the original second optical filter is installed on the installation box, the replacement operation of the second optical filter is convenient and reliable, time and labor are saved, the replacement efficiency of the second optical filter is improved, and the cost is saved.

the embodiment of the utility model provides a liquid chromatography system, including foretell light source subassembly or fluorescence detector, have all advantages of above-mentioned light source subassembly or fluorescence detector.

Drawings

In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings required to be used in the embodiments will be briefly described below, it should be understood that the following drawings only illustrate some embodiments of the present invention, and therefore should not be considered as limiting the scope, and for those skilled in the art, other related drawings can be obtained from the drawings without creative efforts, and the protection scope of the present invention also belongs to the protection scope of the present invention.

Fig. 1 is an exploded schematic view of a light source module provided in this embodiment;

Fig. 2 is a schematic cross-sectional view of a light source module provided in this embodiment;

FIG. 3 is a schematic structural diagram of a fluorescence detector provided in this embodiment;

FIG. 4 is a schematic view of an overall exploded structure of the fluorescence detector provided in this embodiment;

fig. 5 is a schematic structural diagram of the box body provided in this embodiment;

Fig. 6 is a schematic structural diagram of the mounting block provided in this embodiment;

Fig. 7 is a schematic structural diagram of the fixing member provided in this embodiment;

FIG. 8 is a schematic structural view of the fluorescence detector according to this embodiment in an assembling process;

FIG. 9 is a schematic structural view of another assembly process of the fluorescence detector provided in this embodiment;

Fig. 10 is a schematic optical path diagram of the fluorescence detector provided in this embodiment.

Icon: 01-a fluorescence detector; 001-a light source assembly; 100-monochromatic light source; 101-a drive circuit board; 1010 — a first via; 102-a light emitting diode; 110-a base frame; 111-a substrate; 1110-a central aperture; 1111-a second through hole; 112-lens barrel holder; 1120-optical path channel; 1121-a first cavity section; 1122-a second cavity section; 1123-a holding portion; 113-a buffer cover; 1130-optical vias; 120-a first filter; 130-a focusing lens; 002-installing a box; 200-a tank body; 201-a chamber; 202-a first outer wall; 2020-a first opening; 203-a second outer wall; 2030-a second opening; 2031-relief holes; 204-a third outer wall; 2040-adjustment holes; 2041-an installation groove; 2042-plug holes; 2043-screw hole; 2044-first directional section; 210-closing plate; 003-mounting block; 300-a first mounting hole; 310-a second mounting hole; 320-positioning grooves; 330-a positioning wall; 004-flow-through cell; 005-a second filter; 006-fixing member; 600-a fixed plate; 601-mounting a through hole; 602-a second directional section; 610-a fixing strip; 620-locking screws; 007-a photoelectric conversion component; 700-PM T; 701-detection window; 710-C PLD; 008-a condenser lens; 009-first blocking; 010-second plug.

Detailed Description

in order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are some, but not all, embodiments of the present invention. The components of embodiments of the present invention, as generally described and illustrated in the figures herein, may be arranged and designed in a wide variety of different configurations. Thus, the following detailed description of the embodiments of the present invention, presented in the accompanying drawings, is not intended to limit the scope of the invention, as claimed, but is merely representative of selected embodiments of the invention. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative efforts belong to the protection scope of the present invention.

It should be noted that: like reference numbers and letters refer to like items in the following figures, and thus, once an item is defined in one figure, it need not be further defined and explained in subsequent figures.

In the description of the present invention, it should be noted that the terms "center", "inside", "outside", and the like indicate the position or positional relationship based on the position or positional relationship shown in the drawings, or the position or positional relationship that the utility model is usually placed when using, and are only for convenience of describing the present invention and simplifying the description, but do not indicate or imply that the device or element referred to must have a specific position, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first," "second," "third," and the like are used solely to distinguish one from another and are not to be construed as indicating or implying relative importance.

In the description of the present invention, it should also be noted that, unless otherwise explicitly specified or limited, the terms "disposed," "mounted," "connected," and "connected" are to be construed broadly, e.g., as meaning either a fixed connection, a removable connection, or an integral connection; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present invention can be understood in specific cases to those skilled in the art.

It should be noted that, in the present invention, the embodiments and features of the embodiments may be combined with each other without conflict.

in the following embodiments, unless otherwise specified, the direction indicated by ab in fig. 6 indicates the "light propagation direction of the monochromatic light source", and the direction indicated by cd in fig. 6 indicates the "light propagation direction of the fluorescent light emitted to the second filter", preferably, the direction indicated by ab and the direction indicated by cd in the following embodiments are perpendicular to each other, and it should be noted that, in other embodiments, the direction indicated by ab and the direction indicated by cd are not limited to perpendicular to each other, and the direction indicated by ab and the direction indicated by cd may be at an angle of other angles.

Example 1

referring to fig. 1-2, the present embodiment provides a light source assembly 001, where the light source assembly 001 includes a monochromatic light source 100, a base frame 110 and a first optical filter 120, the monochromatic light source 100 and the first optical filter 120 are both mounted on the base frame 110, and the first optical filter 120 is located on a light propagation path of the monochromatic light source 100.

The light source subassembly 001 that this embodiment provided, monochromatic light source 100 and first light filter 120 all install on bed frame 110, and monochromatic light source 100 and first light filter 120 all pass through bed frame 110 location, and the position of the two is accurate, and the light source subassembly 001 during operation reliability is high. Meanwhile, the light source assembly 001 is installed and replaced as an integral structure, so that the step of debugging the relative positions of the monochromatic light source 100 and the first optical filter 120 when the light source assembly 001 is replaced is reduced, the installation process is saved, the time and the labor are saved, the installation and replacement operation is convenient, and the installation efficiency and the replacement efficiency of the light source assembly 001 are improved; and the light source component 001 has compact structure, small volume and small occupied space, and is convenient to transport and store.

referring to fig. 1, in the present embodiment, the monochromatic light source 100 includes a light emitting diode 102 and a driving circuit board 101, the light emitting diode is mounted on the driving circuit board 101, and at least one first through hole 1010 for a bolt to pass through is disposed on the driving circuit board 101.

Alternatively, the driving circuit board 101 is a rectangular board, and obviously, in other embodiments, the driving circuit board 101 may not be limited to be a rectangular board, for example, the driving circuit board 101 may be a circular board, a square board, an elliptical board, or the like. Four first through holes 1010 are formed in the driving circuit board 101, the four first through holes 1010 are respectively close to four corners of the driving circuit board 101, each first through hole 1010 is a circular hole, and obviously, in other embodiments, the shape of the first through hole 1010 may not be limited to be a circular hole; in addition, the number of the first through holes 1010 may not be limited to four, and may be set as needed, which is not listed here.

It should be noted that, in other embodiments, the driving circuit board 101 may not have a through hole for passing a bolt, and the through hole and the component to be mated may be connected by using an adhesive, a welding, or a snap structure.

Optionally, the light emitting diode 102 is mounted at a central position of the driving circuit board 101, and the four first through holes 1010 are uniformly spaced around the light emitting diode 102. In other embodiments, the light emitting diodes 102 may be eccentrically positioned with respect to the drive circuitry.

It should be understood that the monochromatic light source 100 is used to provide monochromatic light, and in other embodiments, the monochromatic light may be laser light, and the monochromatic light source 100 includes a laser generator.

optionally, the first filter 120 is an excitation filter.

Referring to fig. 1 and 2, in the present embodiment, the base frame 110 includes a substrate 111, a lens barrel support 112, and a buffering cover 113. The barrel holder 112 is attached to the base plate 111, and the buffer cover 113 is attached to the barrel holder 112.

Optionally, the substrate 111 is a rectangular plate, and in other embodiments, the substrate 111 may be a circular plate, a square plate, or an elliptical plate. The base plate 111 is provided with a central hole 1110 and at least one second through hole 1111 for a bolt to pass through, and the central hole 1110 can be a circular hole, a square hole or a triangular hole; the second through hole 1111 may be a circular hole, a square hole, a triangular hole, or the like. For example, in the present embodiment, the substrate 111 is provided with a circular central hole 1110 and four circular second through holes 1111, the central hole 1110 is located at the middle position of the substrate 111, and the four second through holes 1111 are respectively close to the four corners of the substrate 111.

Optionally, the lens barrel holder 112 is a cylinder, that is, the cross-sectional shape of the lens barrel holder 112 along the direction perpendicular to the central axis thereof is a circular ring, obviously, in other embodiments, the lens barrel holder 112 may also be a square cylinder or an oval cylinder, etc. The barrel cavity of the lens barrel support 112 is an optical path channel 1120, the optical path channel 1120 includes a first cavity segment 1121 and a second cavity segment 1122 which are communicated and coaxial, an inner diameter of the first cavity segment 1121 is smaller than an inner diameter of the second cavity segment 1122, an annular abutting portion 1123 is formed at a connection position of the first cavity segment 1121 and the second cavity segment 1122, the abutting portion 1123 is, for example and without limitation, an abutting surface, and the abutting surface is, for example and without limitation, an abutting plane; the abutting surface is, for example, but not limited to, a torus. It should be appreciated that in other embodiments, the first and second cavity segments 1121, 1122 may not be coaxially disposed.

in addition, in other embodiments, the abutting portion 1123 may be a protrusion disposed in the optical path channel 1120, the protrusion may be an annular protrusion, and further, the protrusion is an annular protrusion, and an annular end surface of the protrusion is an abutting surface.

Alternatively, in other embodiments, the holding portion 1123 may not be disposed in the optical path 1120.

referring to fig. 1 and 2, the buffering cover 113 may be a circular cover, and obviously, the buffering cover 113 may also be a square cover in other embodiments. The buffer cover 113 is provided with a light path through hole 1130, the light path through hole 1130 is, for example, but not limited to, a circular hole, a central axis of the light path through hole 1130 is collinear with a central axis of the buffer cover 113, and in other embodiments, the central axis of the light path through hole 1130 may not be collinear with the central axis of the buffer cover 113. The buffer cover 113 has a certain elastic deformation capability, for example, in the embodiment, the buffer cover 113 is a rubber cover. Obviously, in other embodiments, the rubber cover may be made of a sponge material.

In the base frame 110 provided by this embodiment, the lens barrel holder 112 is mounted on the substrate 111, the central hole 1110 of the lens barrel holder 112 is communicated with the optical path channel 1120 and coaxially disposed, the aperture of the central hole 1110 is larger than that of the optical path channel 1120, the central hole 1110 and the optical path channel 1120 form a stepped hole, the buffer cover 113 is mounted at one end of the lens barrel holder 112 away from the substrate 111, and the optical path through hole 1130 of the buffer cover 113 is communicated with the optical path channel 1120 and coaxially disposed. In other embodiments, the substrate 111 and the barrel holder 112 may be integrally formed, and the buffer cover 113 may be mounted on the barrel holder 112 by bonding, so that a cavity for placing the first optical filter 120 is formed between the abutting surface and the buffer cover 113.

The assembly process of the light source assembly 001 provided by this embodiment is as follows:

Referring to fig. 2, the driving circuit board 101 with the light emitting diodes 102 mounted thereon is spliced with the substrate 111, the light emitting diode passes through the central hole 1110 of the substrate 111 and extends into the light path channel 1120, and is respectively inserted into the paired first through hole 1010 and the paired second through hole 1111 by using bolts, so that the substrate 111 and the driving circuit board 101 are fixed by using the bolts. The first filter 120 is placed in the optical path channel 1120, one side part of the first filter 120 abuts against the abutting part 1123, then the buffer cover 113 is bonded on the end surface of the lens barrel support 112 away from the substrate 111, one end surface of the buffer cover 113 close to the substrate 111 at least partially abuts against the other side of the first filter 120, the first filter 120 is positioned between the buffer cover 113 and the abutting part 1123, and the movement of the first filter 120 along the length direction of the optical path channel 1120 is limited.

In other embodiments, the light source assembly 001 further comprises a focusing lens 130, and the focusing lens 130 is, for example, a convex lens. The focusing lens 130 is installed in the optical path 1120, the focusing lens 130 abuts against the abutting surface, the first optical filter 120 abuts against the focusing lens 130, and the buffer cover 113 abuts against the first optical filter 120, in other words, the focusing lens 130, the first optical filter 120, and the buffer cover 113 are sequentially arranged along the length direction of the optical path 1120.

In other embodiments, the base frame 110 may not include the buffer cover 113, and the first filter 120 is directly mounted in the optical path channel 1120, for example, the first filter 120 is bonded to the lens barrel support 112 or is in an interference fit.

The light source assembly 001 provided by the embodiment has a compact structure, a small volume and accurate positions of parts; the light source component 001 is installed and replaced as a whole, the operation efficiency is high, and time and labor are saved.

Example 2

Referring to fig. 3 and 4, the present embodiment provides a fluorescence detector 01, wherein the fluorescence detector 01 includes a mounting box 002 and the light source assembly 001 described in embodiment 1, and the light source assembly 001 is mounted on the mounting box 002.

Alternatively, the mounting case 002 is a rectangular parallelepiped case, and obviously, in other embodiments, the mounting case 002 may be a rectangular parallelepiped case, a spherical case, or another case with other shapes. The installation box 002 includes a box body 200 and a cover plate 210.

Referring to fig. 5 in conjunction with fig. 3, optionally, the tank body 200 has a chamber 201, and the tank body 200 has a first outer wall 202, a second outer wall 203, and a third outer wall 204 converging to an apex. The first outer wall 202 is provided with a first opening 2020 communicating with the chamber 201, the first opening 2020 being, for example, but not limited to, a rectangular opening. The second outer wall 203 is provided with a second opening 2030 communicated with the chamber 201 and a relief groove 2031 communicated with the chamber 201, the second opening 2030 is, for example, but not limited to, a rectangular opening, one end of the relief groove 2031 is communicated with the second opening 2030, the other end extends to the first outer wall 202, and the relief groove 2031 is used for a power line to pass through. The third outer wall 204 is provided with an adjusting hole 2040, an installation groove 2041, and an insertion hole 2042 and a threaded hole 2043 located at the bottom of the installation groove 2041, the adjusting hole 2040 is communicated with the chamber 201, and the adjusting hole 2040 may be, but is not limited to, a circular hole. The mounting groove 2041 is, for example and without limitation, a rectangular groove, the inserting hole 2042 is, for example and without limitation, a strip-shaped opening, a length direction of the inserting hole 2042 is parallel to a length direction of the mounting groove 2041, a length direction of the inserting hole 2042 is perpendicular to the second outer wall 203, the inserting hole 2042 has a first direction portion 2044, the first direction portion 2044 may be an arc-shaped corner on a hole wall of the inserting hole 2042, and in other embodiments, the first direction portion 2044 may be an oblique corner disposed on the hole wall of the inserting hole 2042. It should be understood that the number of the first direction portions 2044 is set as needed, for example, in the present embodiment, the number of the first direction portions 2044 is two. The threaded holes 2043 are round blind holes, the number of the threaded holes 2043 is, for example, but not limited to, two, the two threaded holes 2043 are arranged at intervals along the length direction of the mounting groove 2041, and the insertion hole 2042 is located between the two threaded holes 2043.

Referring to fig. 4, the closing plate 210 may be, but is not limited to, a rectangular plate, the closing plate 210 covers the second opening 2030, and the closing plate 210 is fixed to the tank body 200 by screws. It should be understood that in other embodiments, the cover plate 210 is attached to the tank body 200 by adhesive, snap-fit, or welding.

Referring to fig. 4, in the present embodiment, the fluorescence detector 01 further includes an installation block 003, a flow cell 004, a second optical filter 005, a fixing member 006, and a photoelectric conversion element 007. The mounting block 003 is mounted in the case body 200, the flow cell 004 is mounted in the mounting block 003, the second optical filter 005 is mounted in the case body 200, and the photoelectric conversion module 007 is mounted on the case body 200.

Referring to fig. 6, the mounting block 003 is optionally square, and in other embodiments, the mounting block 003 can be other shapes. The mounting block 003 is provided with a first mounting hole 300 and a second mounting hole 310, a central axis of the first mounting hole 300 is perpendicular to a central axis of the second mounting hole 310, and a position where the first mounting hole 300 is communicated with the second mounting hole 310 is located between two ends of the first mounting hole 300. The first mounting hole 300 is, for example and without limitation, a cylindrical hole, the wall of the first mounting hole 300 is provided with a positioning groove 320, the positioning groove 320 is, for example and without limitation, a rectangular groove, the length direction of the positioning groove 320 is parallel to the length direction of the first mounting hole 300, the groove depth direction of the positioning groove 320 is outward along the radial direction of the first mounting hole 300, one end of the positioning groove 320 extends to the end face of one port of the first mounting hole 300, and the other end of the positioning groove 320 is spaced from the other end of the first mounting hole 300. The second mounting hole 310 is, for example, but not limited to, a cylindrical hole, and the end of the second mounting hole 310 away from the first mounting hole 300 extends to the outer wall of the mounting block 003, which is defined as a positioning wall 330, and the central axis of the second mounting hole 310 is perpendicular to the positioning wall 330. Obviously, in other embodiments, the first mounting hole 300 and the second mounting hole 310 may not be perpendicular to each other, and the included angle between the first mounting hole 300 and the second mounting hole 310 may be other angles.

Referring to fig. 4, the flow cell 004 may alternatively be rectangular, and in other embodiments, the flow cell 004 may be cylindrical or have other shapes. The flow-through cell 004 is provided with a fluid inlet (not shown) and a fluid outlet (not shown), and the fluid inlet and the fluid outlet are respectively communicated with a pipeline.

Optionally, the second filter 005 is an emission filter.

Referring to fig. 7, the fixing member 006 may alternatively include a fixing plate 600 and a fixing strip 610 connected thereto. The fixing plate 600 is, for example, but not limited to, a rectangular plate, and the fixing plate 600 is provided with a mounting through hole 601, and the mounting through hole 601 is, for example, but not limited to, a circular hole. The fixing plate 600 is provided with a second direction portion 602 matching the first direction portion 2044, and the second direction portion 602 may be an arc angle provided on the outer wall of the fixing plate 600, or in other embodiments, the second direction portion 602 may be an oblique angle provided on the outer wall of the fixing plate 600. The number of the second direction portions 602 may correspond to the number of the first direction portions 2044. Two locking screws 620 are screwed on the fixing strip 610, and the two locking screws 620 are arranged at intervals along the length direction of the fixing strip 610.

Optionally, the photoelectric conversion module 007 includes a PM T700 (photo-multiplier tube) and a CPLD710 (Complex Program map Logic Device), and the PM T700 is in signal connection with the CPLD 710. The PM T700 is used for converting the optical signal into an electric pulse signal, and the electric pulse signal is transmitted to the CLPD and is output in a form of photon number collected by the CPLD710, so that the intensity of the fluorescent signal is reflected.

In other embodiments, fluorescence detector 01 also includes a condenser 008, such as a convex lens.

The assembly process of the fluorescence detector 01 provided in this embodiment is as follows:

Referring to fig. 4, 8 and 9, the flow cell 004 is installed in the first installation hole 300 of the installation block 003 and is clamped in the positioning groove 320, a first plug 009 is disposed at an end of the first installation through hole 601, the first plug 009 is screwed and fixed with the first installation through hole 601 to prevent the flow cell 004 from falling off from the first installation through hole 601, that is, two sides of the flow cell 004 along the length direction of the first installation hole 300 are respectively abutted against one groove wall of the first plug 009 and one groove wall of the positioning groove 320, so as to fix the flow cell 004 relative to the installation block 003. The pipes connecting the fluid inlet and the fluid outlet of the flow-through cell 004 extend out of the mounting block 003. The mounting block 003 is placed into the box body 200 through the second opening 2030, the mounting block 003 and the box body 200 can be fixedly connected by screws, the first mounting hole 300 on the mounting block 003 is perpendicular to the first outer wall 202, and the second mounting hole 310 on the mounting block 003 is perpendicular to the second outer wall 203. The lens barrel holder 112 of the light source assembly 001 is inserted into the mounting block 003 from the second mounting hole 310, and the buffer cover 113 on the lens barrel holder 112 abuts against the outer wall of the flow cell 004, so that the fluorescence propagated in the flow cell 004 is propagated from the second mounting hole 310. The substrate 111 and the drive circuit board 101 are then fixed to the mounting block 003 with screws. The cover plate 210 is fixed to the second outer wall 203, and the cover plate 210 covers the second opening 2030. Fixing piece 006 to be equipped with condensing lens 008 and second light filter 005 is pegged graft in spliced eye 2042, and fixed plate 600 and spliced eye 2042 sliding fit, during the change, directly extract fixed plate 600, convenient operation. In the plugging process, the first direction portion 2044 corresponds to the second direction portion 602, so that the front and back sides of the second optical filter 005 cannot make mistakes, that is, the light rays firstly pass through the condenser 008 and then irradiate the second optical filter 005, and the normal operation of the device cannot be influenced. When the fixing member 006 is inserted into the groove bottoms of the fixing strip 610 and the positioning groove 320, the mounting through hole 601 is communicated with the first mounting hole 300, the second optical filter 005 is located on a light propagation path of fluorescence emitted from the inside of the flow cell 004, the locking screw 620 corresponds to the screwing hole 2043, and the locking screw 620 is screwed and fixed in the screwing hole 2043, so that the fixing member 006 is fixedly connected with the tank body 200. One side of the PM T700 is covered at the second opening 2030, the PM T700 is fixed to the casing body 200 by screws, and the detection window 701 on the PM T700 communicates with the mounting through hole 601 for receiving the fluorescence emitted from the second filter 005. The box body 200, the closing plate 210, the fixing strip 610 and the PM T700 form a closed cavity 201, and the flow cell 004 is located in the closed cavity 201, so that a good light shielding environment is provided for fluorescence detection. In addition, the second sealing plug 010 may be disposed at the adjusting hole 2040, and when the resistance value of the sliding rheostat mounted on the driving circuit board 101 needs to be adjusted, the second sealing plug 010 may be pulled out and a tool such as a screwdriver may be used to insert the sliding rheostat from the adjusting hole 2040.

It should be noted that the above assembling process is only an embodiment, and is not a limitation on the assembling order of the fluorescence detector 01, and other orders and methods capable of completing the assembling of the fluorescence detector 01 are not listed.

the operation flow of the fluorescence detector 01 provided by the embodiment is as follows:

Referring to fig. 10, the driving circuit board 101 transmits current to the light emitting diode 102 to enable the light emitting diode 102 to output stable monochromatic light, the monochromatic light sequentially passes through the focusing lens 130, the first optical filter 120 and the flow cell 004, at this time, if a separated sample is mixed in a flowing phase flowing through the flow cell 004, fluorescence is generated under excitation of the corresponding monochromatic light, a fluorescence signal is collected and collimated by the collecting lens 008, and is received by the detection window 701 of the PM T700 after stray light is filtered by the emission optical filter, the PMT700 converts the optical signal into an electrical pulse signal, and the electrical pulse signal is finally collected by the CPLD710 and output in the form of photon number, so that the intensity of the fluorescence signal is reflected.

In this embodiment, the light propagation direction of the monochromatic light source 100 and the light propagation direction of the fluorescence emitted to the second optical filter 005 form an included angle of 90 degrees, and in other embodiments, the included angle between the light propagation direction of the monochromatic light source 100 and the light propagation direction of the fluorescence emitted to the second optical filter 005 may be other angles.

The fluorescence detector 01 provided by the embodiment has a simple structure, is convenient for replacing the light source component 001 and the second optical filter 005, and is low in cost.

example 3

This embodiment provides a liquid chromatography system including the light source assembly 001 described in embodiment 1 or the fluorescence detector 01 described in embodiment 2.

The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention, and various modifications and changes may be made by those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims (10)

1. The light source component is characterized by comprising a monochromatic light source, a base frame and a first optical filter, wherein the monochromatic light source and the first optical filter are arranged on the base frame, and the first optical filter is positioned on a light propagation path of the monochromatic light source.

2. The light source assembly of claim 1, wherein the base frame comprises a base plate and a lens barrel holder connected to each other, the monochromatic light source is mounted on the base plate, the lens barrel holder has a light path, and the first optical filter is mounted in the light path.

3. The light source assembly of claim 2, wherein the base frame further includes a buffer cover for preventing the first filter from falling off from a port of the optical path channel away from the substrate, the buffer cover has an optical path through hole, the buffer cover is mounted on the lens barrel holder, and the optical path channel is communicated with the optical path through hole.

4. The light source assembly according to claim 3, wherein an abutting portion is provided on an inner wall of the light path channel, a gap is provided between the abutting portion and an end of the light path channel away from the substrate, and the first filter abuts against the abutting portion and the buffer cover at the same time.

5. a fluorescence detector comprising a mounting box having a closed chamber and a light source assembly according to any of claims 1-4, the base frame being mounted on the mounting box, the monochromatic light source and the first optical filter being located within the closed chamber.

6. The fluorescence detector of claim 5, further comprising a flow cell, a second optical filter, and a photoelectric conversion assembly, wherein the flow cell and the second optical filter are both mounted within the closed chamber, the flow cell is located in a light propagation path of the monochromatic light source, the second optical filter is located in a light propagation path of the fluorescence emitted from within the flow cell, and a light propagation direction of the monochromatic light source forms an angle with a light propagation direction of the fluorescence emitted toward the second optical filter; the photoelectric conversion assembly is arranged on the installation box and used for receiving the fluorescence which passes through the second optical filter.

7. The fluorescence detector of claim 6, further comprising an installation block, wherein the installation block is installed in the closed chamber, the installation block is provided with a first installation hole and a second installation hole, the first installation hole is communicated with the second installation hole and has an included angle, the flow cell is installed in the first installation hole, and the monochromatic light source is inserted into the second installation hole.

8. The fluorescence detector of claim 6, further comprising a mount, wherein the second filter is mounted to the mount, and wherein the mount is removably coupled to the mounting housing.

9. The fluorescence detector according to claim 8, wherein the fixing member comprises a fixing plate and a fixing strip connected to each other, the fixing plate is provided with a mounting through hole, and the second filter is mounted in the mounting through hole; the mounting box is provided with an inserting hole communicated with the closed cavity, the fixing plate is in sliding fit with the inserting hole, and the fixing strip is detachably connected with the mounting box.

10. A liquid chromatography system comprising a light source assembly according to any of claims 1-4 or a fluorescence detector according to any of claims 5-9.