CN109738393B - Optical detection device and specific protein analyzer - Google Patents
Optical detection device and specific protein analyzer Download PDFInfo
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- CN109738393B CN109738393B CN201910022037.7A CN201910022037A CN109738393B CN 109738393 B CN109738393 B CN 109738393B CN 201910022037 A CN201910022037 A CN 201910022037A CN 109738393 B CN109738393 B CN 109738393B
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Abstract
The invention relates to an optical detection device and a specific protein analyzer, the optical detection device comprises a detection body, a light source mechanism and a detection assembly, the detection body is provided with a light incidence channel, a light propagation channel, a transmission light receiving channel and a containing hole, the containing hole is used for containing a container for accommodating a sample to be detected, the transmission light receiving channel is arranged on the wall part of the detection body for limiting the light propagation channel, the light source mechanism is arranged on the detection body, the detection assembly comprises a first convex lens, a reflector, a second convex lens, a first photoelectric receiving device and a second photoelectric receiving device, the first convex lens, the reflector and the second convex lens are sequentially arranged in the light propagation channel, the first photoelectric receiving device is arranged at one end of the light propagation channel, the second photoelectric receiving device is arranged at one end of the transmission light receiving channel and used for receiving the penetrating light. The device can realize transmission light detection function and scattered light detection function simultaneously, and detection efficiency is high, and detection effect is good.
Description
Technical Field
The invention relates to the field of in-vitro diagnosis medical instruments, in particular to an optical detection device and a specific protein analyzer with the same.
Background
A specific protein analyzer is an instrument mainly used in laboratories for analyzing and detecting proteins in various samples, and an optical detection device is usually used for detecting protein content.
The optical detection device typically includes a transmission detection module and a scattering detection module. At present, in the existing optical detection device, most of the transmission detection modules and the scattering detection modules are in non-integrated structures, detection needs to be performed step by step, and detection efficiency is low.
In addition, most of the existing transmission detection modules and scattering detection modules adopt fixed and unchangeable light holes to guide light, and cannot adjust transmitted light and scattered light according to the difference of samples to be detected, so that the universality is poor, and the detection effect on the samples is poor.
Disclosure of Invention
In view of the above-mentioned drawbacks of the prior art, an object of the present invention is to provide an optical detection device integrating a transmission light detection function and a scattered light detection function, and a specific protein analyzer having the optical detection device.
Therefore, the invention provides the following technical scheme.
The invention provides an optical detection device, which comprises a detection body, a light source mechanism and a detection assembly,
the detection body is provided with a light incidence channel, a light transmission channel, a transmission light receiving channel and an accommodating hole,
the accommodating hole is arranged between the light incidence channel and the light propagation channel and is used for accommodating a container for accommodating a sample to be detected,
the transmitted light receiving channel opens to a wall portion of the detection body that defines the light propagation channel,
the light source mechanism is arranged on the detection body, when the container provided with the sample to be detected is arranged in the accommodating hole, the light emitted by the light source mechanism can irradiate the container through the light incidence channel, the generated transmitted light and scattered light enter the light propagation channel,
the detection assembly comprises a first convex lens, a reflector, a second convex lens, a first photoelectric receiving device and a second photoelectric receiving device,
the first convex lens, the reflector and the second convex lens are arranged in the light propagation channel in sequence along the extending direction of the light propagation channel,
a space through which the scattered light passes is formed between the outer periphery of the mirror and the wall portion,
the first photoelectric receiving device is arranged at one end of the light propagation channel, which is opposite to the accommodating hole, and is used for receiving scattered light which sequentially passes through the first convex lens and the second convex lens,
the second photoelectric receiving device is arranged at one end of the transmission light receiving channel, which is far away from the light propagation channel, and is used for receiving the transmission light which passes through the first convex lens and is reflected by the reflector.
In at least one embodiment, the detection assembly further comprises a first planar lens, a second planar lens, and a septum,
the first planar lens and the second planar lens are arranged at intervals along the extension direction, the reflector is respectively connected with the first planar lens and the second planar lens and is positioned between the first planar lens and the second planar lens in the extension direction, the partition pipe comprises a first partition pipe, a second partition pipe and a third partition pipe,
the first diaphragm is arranged between the first convex lens and the first plane lens,
the second diaphragm is arranged between the second planar lens and the second convex lens,
the third isolation tube is arranged between the second convex lens and the first photoelectric receiving device,
the light propagation channel is provided with a limiting part,
the first photoelectric receiving device abuts against the third spacing tube, so that the third spacing tube abuts against the second convex lens, the second spacing tube abuts against the second planar lens, the first spacing tube abuts against the first convex lens, and the first convex lens abuts against the limiting part.
In at least one embodiment, the light propagation channel includes a tapered hole and a cylindrical hole connected to each other, one end of the tapered hole is communicated with the accommodation hole, the other end of the tapered hole is communicated with the cylindrical hole to form the limiting portion, and the tapered hole is formed such that the diameter of the tapered hole is larger as the tapered hole is closer to the cylindrical hole.
In at least one embodiment, the first planar lens is provided with a first mounting hole, the second planar lens is provided with a second mounting hole, and the reflector is connected to a hole wall of the first mounting hole and a hole wall of the second mounting hole respectively.
In at least one embodiment, the detection assembly further includes a positioning mounting plate, one end of which abuts against the third bulkhead and the other end of which abuts against the first photo-receiving device.
In at least one embodiment, the light source mechanism is inserted into the light incident channel, and an adjustment gap exists between the light source mechanism and a wall surface of the detection body defining the light incident channel.
In at least one embodiment, the transmitted light receiving channel is disposed through the wall portion perpendicular to the light propagation channel.
In at least one embodiment, the light source mechanism includes a laser and a mount through which the laser is mounted to the detection body.
In at least one embodiment, the receiving opening has a rectangular cross section and/or the projection of the mirror in the extension direction has a circular shape.
The invention also provides a specific protein analyzer, which comprises the optical detection device in any one of the above embodiments.
By adopting the technical scheme, the invention provides the optical detection device, through the mutual matching of the detection body, the light source mechanism and the detection assembly, the transmission light detection function and the scattered light detection function can be realized simultaneously, and the detection efficiency is high. In addition, by arranging the first convex lens, the reflector and the second convex lens, the intensity of the transmitted light beam can be adjusted and the scattered light with a required angle can be obtained by reasonably setting the size of the reflector, so that the detection effect is improved.
It will be appreciated that a particular protein analyzer having such an optical detection device would have the same beneficial effects.
Drawings
Fig. 1 is a perspective view showing an angle of an optical detection apparatus according to the present invention.
Fig. 2 is a perspective view showing another angle of the optical detection apparatus according to the present invention.
Fig. 3 shows a cross-sectional view of fig. 1.
Fig. 4 shows an exploded view of the light source mechanism and detection assembly of fig. 1.
Description of the reference numerals
1, detecting a body; 11 light incident channel; 12 a light propagation channel; 121 tapered holes; 122 cylindrical holes; 123 limit wall surfaces; 13 a light-receiving channel; 14 receiving holes;
2 a light source mechanism; 21 a laser; 211 adjusting the gap; 22 a mounting seat;
3, a detection component; 31 a first convex lens; 32 a first planar lens; 321 a first mounting hole; 33 a mirror; 34 a second planar lens; 341 second mounting hole; 35 a second convex lens; 36 positioning the mounting plate; 361 a substrate part; 362 cylindrical portion; 37 a first photoelectric receiving plate; 371 a first photovoltaic cell; 38 a second photoelectric receiving plate; 381 a second photovoltaic cell; 39 separating the tubes; 391 a first tubephram; 392 a second tubephram; 393 a third tubephram;
4, and (4) containers.
Detailed Description
Exemplary embodiments of the present invention are described below with reference to the accompanying drawings. It should be understood that the detailed description is only intended to teach one skilled in the art how to practice the invention, and is not intended to be exhaustive or to limit the scope of the invention.
References to "left", "right", "upper" and "lower" in this invention are based on the perspective of FIG. 1.
An embodiment of the optical detection apparatus according to the present invention is described in detail below with reference to fig. 1 to 4.
In the present embodiment, as shown in fig. 1, the optical detection apparatus includes a detection body 1, a light source mechanism 2, and a detection unit 3.
In the present embodiment, as shown in fig. 2 and 3, the entire detection body 1 has a substantially rectangular parallelepiped shape, and the detection body 1 includes a light incident channel 11, a light propagation channel 12, a transmitted light receiving channel 13, and a housing hole 14.
In the present embodiment, as shown in fig. 3, the light incident channel 11 extends from the left end surface of the detection body 1 toward the right side. Wherein the light incident channel 11 has a circular cross-section.
In the present embodiment, as shown in fig. 3, the light propagation channel 12 includes a tapered hole 121, a cylindrical hole 122, and a stopper wall surface 123 (stopper portion). Wherein, cylindrical hole 122 extends towards the left side from the right-hand member face that detects body 1 and sets up, and the right-hand member of bell mouth 121 links to each other with cylindrical hole 122, and the left end and the accommodation hole 14 intercommunication of bell mouth 121, the department that links to each other of bell mouth 121 and cylindrical hole 122 forms spacing wall 123.
In the present embodiment, the tapered hole 121 is formed so that the diameter thereof becomes larger as it approaches the cylindrical hole 122. Thus, appropriately setting the taper of the tapered hole 121 makes it possible to cause scattered light having a scattering angle within a predetermined range to enter the light propagation channel 12.
In the present embodiment, the tapered hole 121 and the cylindrical hole 122 are both circular in cross section.
In the present embodiment, as shown in fig. 3, the wall portion of the detection body 1 defining the cylindrical hole 122 opens the transmission light receiving channel 13, and the transmission light receiving channel 13 is perpendicular to the cylindrical hole 122. Wherein the cross section of the transmitted light receiving channel 13 is circular.
In the present embodiment, as shown in fig. 3, the accommodation hole 14 is provided between the light incident channel 11 and the light propagation channel 12, and communicates with both the light incident channel 11 and the light propagation channel 12. Wherein the receiving hole 14 has a rectangular cross section, and the receiving hole 14 is used for receiving the container 4 for accommodating the sample to be detected. In this embodiment, the container 4 is a cuvette.
In the present embodiment, as shown in fig. 3, the light source mechanism 2 includes a laser 21 and a mount 22. The laser 21 is partially inserted into the light incident channel 11, a portion of the laser 21 outside the light incident channel 11 is connected to the mounting seat 22, and the laser 21 is mounted to the inspection body 1 through the mounting seat 22.
In the present embodiment, as shown in fig. 3, there is an adjustment gap 211 between the laser 21 and the light incident channel 11. In this way, the direction of the optical axis of the laser 21 can be fine-tuned as necessary, and further, a better received light intensity can be obtained.
In the present embodiment, as shown in fig. 3 and 4, the detection unit 3 includes a first convex lens 31, a first planar lens 32, a reflecting mirror 33, a second planar lens 34, a second convex lens 35, a positioning mounting plate 36, a first photo-receiving plate 37 (first photo-receiving device), a second photo-receiving plate 38 (second photo-receiving device), and a diaphragm 39.
In the present embodiment, as shown in fig. 3, a first convex lens 31, a first planar lens 32, a reflecting mirror 33, a second planar lens 34, and a second convex lens 35 are provided in the left-right direction in the cylindrical hole 122 in this order, a positioning mounting plate 36 and a first photo-electric receiving plate 37 are provided at the right end of the detection body 1, and a second photo-electric receiving plate 38 is provided at an end of the transmitted light receiving channel 13 that faces away from the cylindrical hole 122. The first convex lens 31, the first planar lens 32, the second planar lens 34, and the second convex lens 35 are vertically disposed, and the reflecting mirror 33 is obliquely extended with respect to the first planar lens 32.
In the present embodiment, the mirror 33 has a circular shape in a projection in the left-right direction.
In the present embodiment, as shown in fig. 3 and 4, the first planar lens 32 is provided with a first mounting hole 321, and the second planar lens 34 is provided with a second mounting hole 341. The first mounting hole 321 is opened in the left-right direction at the center of the first planar lens 32, and the second mounting hole 341 is opened in the left-right direction at the center of the second planar lens 34.
In the present embodiment, as shown in fig. 3 and 4, the upper end of the reflector 33 is bonded to the upper hole wall of the first mounting hole 321, and the lower end of the reflector 33 is bonded to the lower hole wall of the second mounting hole 341. In this way, the first plane lens 32, the reflecting mirror 33, and the second plane lens 34 are integrated, and a space through which the scattered light flux in a ring shape passes is formed between the outer periphery of the reflecting mirror 33 and the inner wall surface of the baffle 39.
In the present embodiment, as shown in fig. 3 and 4, the spacer 39 includes a first spacer 391, a second spacer 392, and a third spacer 393. The first spacing tube 391 is disposed between the first convex lens 31 and the first planar lens 32, the second spacing tube 392 is disposed between the second planar lens 34 and the second convex lens 35, and the third spacing tube 393 is disposed between the second convex lens 35 and the positioning and mounting plate 36.
In the present embodiment, as shown in fig. 3 and 4, the positioning attachment plate 36 includes a base plate portion 361 and a cylindrical portion 362. Here, the base plate portion 361 has a rectangular cross section, and the cylindrical portion 362 projects leftward from the left end surface of the base plate portion 361.
In the present embodiment, as shown in fig. 3 and 4, the substrate portion 361 is fixedly attached to the right end portion of the detection body 1 by a fastener, and the cylindrical portion 362 is inserted into the light propagation channel 12 and abuts against the third bulkhead 393.
In the present embodiment, as shown in fig. 3 and 4, the first photoelectric receiving panel 37 is provided with a first photoelectric cell 371 for receiving scattered light for detection.
In the present embodiment, as shown in fig. 3 and 4, the second photoelectric receiving plate 38 is provided with a second photoelectric cell 381 for receiving transmitted light for detection.
In the present embodiment, as shown in fig. 3 and 4, the first photoelectric receiving plate 37 is fixedly attached to the right end portion of the detection body 1 together with the positioning attachment plate 36 by a fastener such that the left end of the cylindrical portion 362 abuts against the third bulkhead 393, the left end of the third bulkhead 393 abuts against the second convex lens 35, the left end of the second bulkhead 392 abuts against the second planar lens 34, the left end of the first bulkhead 391 abuts against the first convex lens 31, and the first convex lens 31 abuts against the stopper wall surface 123.
The operation of the optical detection apparatus according to the present invention will be briefly described below.
In the present embodiment, when the container 4 to which the sample to be tested is mounted is disposed in the accommodating hole 14, the laser 21 emits laser light which is irradiated to the container 4 and passes through the sample in the container 4 to form transmitted light and scattered light entering the light propagation channel 12.
The transmitted light passes through the first convex lens 31, the first mounting hole 321, is reflected by the reflecting mirror 33 into the transmitted light receiving channel 13, and is finally received and processed by the second light receiving plate 38.
The scattered light passes through the first convex lens 31, the first planar lens 32, the second planar lens 34, and the second convex lens 35 and is received and processed by the first photoelectric receiving plate 37.
By adopting the technical scheme, the optical detection device at least has the following advantages:
(1) in the optical detection device of the present invention, the transmitted light detection function and the scattered light detection function are integrated, and the detection efficiency can be improved.
(2) In the optical detection device of the present invention, by providing the first convex lens, the reflecting mirror, and the second convex lens, the intensity of the transmitted light beam and the scattered light at a desired angle can be adjusted by appropriately setting the size of the reflecting mirror, and the detection effect can be further improved.
(3) In the optical detection device, the first convex lens, the first planar lens, the reflector, the second planar lens and the second convex lens are fixed in an abutting mode by arranging the limiting wall surface, the partition pipe and the positioning mounting plate, so that the reflectors with different sizes can be conveniently replaced according to needs to adjust the intensity of transmitted light received by the second photoelectric receiving plate, and the sensitivity and the resolution of transmitted light detection are improved; meanwhile, the convex lens with different curvatures and the partition pipes with different diameters can be conveniently replaced to adjust the scattered light with a required angle (the length of the partition pipe is correspondingly required to be adjusted while the curvature of the convex lens is adjusted), and the detection effect of scattered light detection is improved.
(4) In the optical detection device, an adjusting gap exists between the laser and the wall surface of the detection body for limiting the light incidence channel, so that the optical axis of the laser can be finely adjusted, and the detection effect is improved.
The above embodiments have described the technical solutions of the present invention in detail, but it should be added that:
(1) although it is described in the above embodiment that the cross sections of the tapered hole and the cylindrical hole are circular, the present invention is not limited thereto, and the cross sections of the tapered hole and the cylindrical hole may be polygonal.
(2) Although it is described in the above embodiment that the light source mechanism includes a laser, the present invention is not limited thereto, and an LED light source may be employed instead of the laser.
(3) Although the laser is partially inserted into the light incident channel in the above embodiment, the present invention is not limited thereto, and the laser may be entirely disposed outside the light incident channel.
(4) Although it is described in the above-described embodiment that the transmitted light receiving channel is perpendicular to the cylindrical hole and has a circular cross section, the present invention is not limited thereto, and the transmitted light receiving channel may extend obliquely with respect to the cylindrical hole, and the cross section of the transmitted light receiving channel may be polygonal.
(5) Although the projection of the mirror in the left-right direction is described as a circle in the above embodiment, the present invention is not limited thereto, and the projection of the mirror in the left-right direction may be a polygon.
(6) Although the first photoelectric receiving plate is mounted on the right end of the detection body through the positioning mounting plate and the positioning mounting plate abuts against the third spacing tube in the above embodiment, the present invention is not limited thereto, and the positioning mounting plate may not be provided so that the first photoelectric receiving plate directly abuts against the third spacing tube.
In addition, the invention also provides a specific protein analyzer which comprises the optical detection device.
It will be appreciated that a particular protein analyzer having such an optical detection device would have the same beneficial effects.
Claims (10)
1. An optical detection device comprises a detection body, a light source mechanism and a detection assembly,
the detection body is provided with a light incidence channel and a light propagation channel,
the detection assembly comprises a first convex lens, a reflector, a second convex lens, a first photoelectric receiving device and a second photoelectric receiving device,
the detection body is also provided with a transmission light receiving channel and a containing hole,
the accommodating hole is arranged between the light incidence channel and the light propagation channel and is used for accommodating a container for accommodating a sample to be detected,
the transmitted light receiving channel opens to a wall portion of the detection body that defines the light propagation channel,
the light source mechanism is arranged on the detection body, when the container provided with the sample to be detected is arranged in the accommodating hole, the light emitted by the light source mechanism can irradiate the container through the light incidence channel, the generated transmitted light and scattered light enter the light propagation channel,
the first convex lens, the reflector and the second convex lens are arranged in the light propagation channel in sequence along the extending direction of the light propagation channel,
a space through which the scattered light passes is formed between the outer periphery of the mirror and the wall portion,
the first photoelectric receiving device is arranged at one end of the light propagation channel, which is opposite to the accommodating hole, and is used for receiving scattered light which sequentially passes through the first convex lens and the second convex lens,
the second photoelectric receiving device is arranged at one end of the transmission light receiving channel, which is far away from the light propagation channel, and is used for receiving the transmission light which passes through the first convex lens and is reflected by the reflector.
2. The optical inspection device of claim 1, wherein the inspection assembly further includes a first planar lens, a second planar lens, and a spacer tube,
the first planar lens and the second planar lens are spaced apart along the extension direction,
the reflector is respectively connected with the first planar lens and the second planar lens, and the reflector is positioned between the first planar lens and the second planar lens in the extension direction,
the partition pipe comprises a first partition pipe, a second partition pipe and a third partition pipe,
the first diaphragm is arranged between the first convex lens and the first plane lens,
the second diaphragm is arranged between the second planar lens and the second convex lens,
the third isolation tube is arranged between the second convex lens and the first photoelectric receiving device,
the light propagation channel is provided with a limiting part,
the first photoelectric receiving device abuts against the third spacing tube, so that the third spacing tube abuts against the second convex lens, the second spacing tube abuts against the second planar lens, the first spacing tube abuts against the first convex lens, and the first convex lens abuts against the limiting part.
3. The optical inspection device of claim 2, wherein the light propagation channel includes a tapered hole and a cylindrical hole connected to each other,
one end of the conical hole is communicated with the accommodating hole, the other end of the conical hole is communicated with the cylindrical hole to form the limiting part,
the tapered hole is formed such that the diameter of the hole becomes larger as it gets closer to the cylindrical hole.
4. The optical inspection device of claim 2, wherein the first planar lens is provided with a first mounting hole, the second planar lens is provided with a second mounting hole, and the reflector is connected to the hole wall of the first mounting hole and the hole wall of the second mounting hole respectively.
5. The optical inspection device of claim 2, wherein the inspection assembly further includes a positioning mounting plate,
one end of the positioning mounting plate is abutted to the third partition pipe, and the other end of the positioning mounting plate is abutted to the first photoelectric receiving device.
6. The optical inspection device according to claim 1, wherein the light source mechanism is inserted into the light incident channel, and an adjustment gap is provided between the light source mechanism and a wall surface of the inspection body defining the light incident channel.
7. The optical inspection device of claim 1, wherein the transmitted light receiving channel is disposed through the wall portion perpendicular to the light propagation channel.
8. The optical inspection device of claim 1, wherein the light source mechanism includes a laser and a mount, the laser being mounted to the inspection body through the mount.
9. Optical detection device according to claim 1, characterized in that the receiving hole is rectangular in cross-section and/or the projection of the mirror in the extension direction is circular.
10. A specific protein analyzer, characterized in that it comprises an optical detection device according to any one of claims 1 to 9.
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| CN201910022037.7A CN109738393B (en) | 2019-01-10 | 2019-01-10 | Optical detection device and specific protein analyzer |
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| CN201910022037.7A CN109738393B (en) | 2019-01-10 | 2019-01-10 | Optical detection device and specific protein analyzer |
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| US6586193B2 (en) * | 1996-04-25 | 2003-07-01 | Genicon Sciences Corporation | Analyte assay using particulate labels |
| CN1384361A (en) * | 2001-04-27 | 2002-12-11 | 刘岩 | Separate multi-channel biochemical analyzer |
| WO2007004466A1 (en) * | 2005-07-01 | 2007-01-11 | Sysmex Corporation | Analyzer |
| JP5078004B2 (en) * | 2007-06-15 | 2012-11-21 | 国立大学法人 香川大学 | Spectroscopic measurement apparatus and spectral measurement method |
| JP4925133B2 (en) * | 2008-01-29 | 2012-04-25 | 独立行政法人産業技術総合研究所 | Terahertz electron beam spectroscopy method and apparatus |
| JP5422990B2 (en) * | 2008-12-22 | 2014-02-19 | 住友電気工業株式会社 | Biological component detection device |
| US20120225475A1 (en) * | 2010-11-16 | 2012-09-06 | 1087 Systems, Inc. | Cytometry system with quantum cascade laser source, acoustic detector, and micro-fluidic cell handling system configured for inspection of individual cells |
| CN102499647B (en) * | 2011-11-14 | 2013-11-27 | 重庆大学 | Multi-mode low-coherence scattering spectrometer |
| ES2907974T3 (en) * | 2013-03-01 | 2022-04-27 | Genea Ip Holdings Pty Ltd | Apparatus, method and system for monitoring the development of cultured samples |
| CN203572827U (en) * | 2013-06-26 | 2014-04-30 | 无锡和瑞盛光电科技有限公司 | Blood cell analysis device |
| US10184892B2 (en) * | 2014-10-29 | 2019-01-22 | Horiba Instruments Incorporated | Determination of water treatment parameters based on absorbance and fluorescence |
| CN105181561B (en) * | 2015-09-02 | 2018-04-24 | 江苏大学 | A kind of blood cell analysis sensor |
| JP6710535B2 (en) * | 2016-02-18 | 2020-06-17 | 株式会社日立ハイテク | Automatic analyzer |
| CN107045637B (en) * | 2016-12-16 | 2020-07-24 | 中国医学科学院生物医学工程研究所 | Blood species identification instrument and method based on spectrum |
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