CN109738393A - Optical detection apparatus and specific protein analyzer - Google Patents
Optical detection apparatus and specific protein analyzer Download PDFInfo
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- CN109738393A CN109738393A CN201910022037.7A CN201910022037A CN109738393A CN 109738393 A CN109738393 A CN 109738393A CN 201910022037 A CN201910022037 A CN 201910022037A CN 109738393 A CN109738393 A CN 109738393A
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Abstract
The present invention relates to a kind of optical detection apparatus and specific protein analyzers, the optical detection apparatus includes detection body, light source mechanism and detection components, detection body is provided with light incidence channel, light propagation channel, transmitted light receiving channel and receiving hole, receiving hole is for accommodating the container for installing sample to be detected, transmitted light receiving channel is opened in the wall portion in the restriction light propagation channel of detection body, light source mechanism is installed on detection body, detection components include the first convex lens, reflecting mirror, second convex lens, first photoelectric receiving arrangement and the second photoelectric receiving arrangement, first convex lens, reflecting mirror and the second convex lens are set in turn in light propagation channel, first photoelectric receiving arrangement is set to the one end in light propagation channel, light is scattered for receiving, second photoelectric receiving arrangement is set to one end of transmitted light receiving channel, it is penetrated for receiving Penetrate light.Optical detection apparatus of the invention can be achieved at the same time transmitted light detection function and scattering light detection function, and detection efficiency is high, and detection effect is good.
Description
Technical field
The present invention relates to in-vitro diagnosis medical instruments field more particularly to a kind of optical detection apparatus and there is optics inspection
Survey the specific protein analyzer of device.
Background technique
Specific protein analyzer is a kind of instrument for being mainly used for laboratory and carrying out the analysis and detection of albumen in various samples
Device generallys use optical detection apparatus and detects to protein content.
Optical detection apparatus generally includes transmission detection module and scatters detection module.Currently, in existing optical detection
In device, transmission detection module and scattering detection module most non-integral structure need substep to carry out when detection, detection efficiency
It is low.
In addition, mostly using changeless unthreaded hole to carry out light greatly in existing transmission detection module and scattering detection module
Guidance cannot be adjusted transmitted light and scattering light according to the different of sample to be detected, poor universality, the detection to sample
It is ineffective.
Summary of the invention
Based on drawbacks described above in the prior art, the purpose of the present invention is to provide one kind to be integrated with transmitted light detection function
Optical detection apparatus with scattering light detection function and specific protein analyzer with the optical detection apparatus.
For this purpose, the present invention provides the following technical solutions.
The present invention provides a kind of optical detection apparatus, the optical detection apparatus include detection body, light source mechanism and
Detection components,
The detection body is provided with light incidence channel, light propagation channel, transmitted light receiving channel and receiving hole,
The receiving hole is set between the smooth incidence channel and the light propagation channel, to be detected for accommodating installing
The container of sample,
The transmitted light receiving channel is opened in the wall portion in the restriction light propagation channel of the detection body,
The light source mechanism is installed on the detection body, and when the container for installing sample to be detected be set to it is described
When in receiving hole, the light that the light source mechanism issues can expose to the container by the smooth incidence channel, generated
Transmitted light and scattering light enter the light propagation channel,
The detection components include the first convex lens, reflecting mirror, the second convex lens, the first photoelectric receiving arrangement and the second light
Electric reception device,
First convex lens, the reflecting mirror and second convex lens along the light propagation channel extending direction
It is set in turn in the light propagation channel,
The space passed through for the scattering light is formed between the periphery of the reflecting mirror and the wall portion,
First photoelectric receiving arrangement is set to the one end opposite with the receiving hole in the light propagation channel, is used for
The scattering light for sequentially passing through first convex lens and second convex lens is received,
Second photoelectric receiving arrangement be set to the transmitted light receiving channel away from the one of the light propagation channel
End, for receiving across first convex lens and the transmitted light that is reflected by the reflecting mirror.
In at least one embodiment, the detection components further include the first planar lens, the second planar lens and every
Pipe,
First planar lens and second planar lens are spaced apart along the extending direction to be arranged, the reflection
Mirror is separately connected with first planar lens and second planar lens, and the reflecting mirror is upper in the extending direction
Between first planar lens and second planar lens, the pipe includes the first pipe, the second pipe and third
Pipe,
First pipe is set between first convex lens and first planar lens,
Second pipe is set between second planar lens and second convex lens,
The third pipe is set between second convex lens and first photoelectric receiving arrangement,
The light propagation channel is provided with limiting section,
First photoelectric receiving arrangement pushes against the third pipe, so that the third pipe abuts second convex lens
Mirror, second pipe abut second planar lens, and first pipe abuts first convex lens, and described first is convex
Lens abut the limiting section.
In at least one embodiment, the light propagation channel includes bellmouth and cylindrical hole interconnected, described
One end of bellmouth is connected to the receiving hole, and the other end and the cylindrical hole connectivity part form the limiting section, the taper
Hole is formed as more bigger close to the cylindrical hole aperture.
In at least one embodiment, the first mounting hole, second planar lens is arranged in first planar lens
Second mounting hole is set, and the reflecting mirror connects respectively with the hole wall of first mounting hole and the hole wall of second mounting hole
It connects.
In at least one embodiment, the detection components further include positioning mounting plate, and the one of the positioning mounting plate
End abuts the third pipe, and the other end is abutted with first photoelectric receiving arrangement.
In at least one embodiment, the light source mechanism is inserted in the smooth incidence channel, and the light source mechanism
There is adjustment gap between the wall surface of the restriction smooth incidence channel of the detection body.
In at least one embodiment, the transmitted light receiving channel is perpendicular to the light propagation channel through described
Wall portion setting.
In at least one embodiment, the light source mechanism includes laser and mounting base, and the laser passes through institute
It states mounting base and is installed on the detection body.
In at least one embodiment, the cross section of the receiving hole is that rectangle and/or the reflecting mirror prolong described in
That stretches direction is projected as circle.
The present invention also provides a kind of specific protein analyzer, which includes any of the above-described embodiment
The optical detection apparatus.
By using above-mentioned technical solution, the present invention provides a kind of optical detection apparatus, pass through detection body, light source
The mutual cooperation of mechanism and detection components can be achieved at the same time transmitted light detection function and scattering light detection function, detection efficiency
It is high.In addition, by the first convex lens of setting, reflecting mirror and the second convex lens, can by the way that the size of reflecting mirror is reasonably arranged,
Adjustment transmitted light beam intensity and the scattering light for obtaining required angle, and then improve detection effect.
It is appreciated that the specific protein analyzer with the optical detection apparatus has same beneficial effect.
Detailed description of the invention
Fig. 1 shows the three-dimensional structure diagram of an angle of optical detection apparatus according to the present invention.
Fig. 2 shows the three-dimensional structure diagrams of another angle of optical detection apparatus according to the present invention.
Fig. 3 shows the cross-sectional view of Fig. 1.
Fig. 4 shows the explosive view of light source mechanism and detection components in Fig. 1.
Description of symbols
1 detection body；11 smooth incidence channels；12 light propagation channels；121 bellmouths；122 cylindrical holes；123 limit wall surfaces；
13 transmitted light receiving channels；14 receiving holes；
2 light source mechanisms；21 lasers；211 adjustment gaps；22 mounting bases；
3 detection components；31 first convex lenses；32 first planar lens；321 first mounting holes；33 reflecting mirrors；34 second is flat
Face lens；341 second mounting holes；35 second convex lenses；36 positioning mounting plates；361 baseplate parts；362 cylindrical parts；37 first photoelectricity
Receiver board；371 first photocells；38 second opto-electronic receiver plates；381 second photocells；39 pipes；391 first pipes；392
Two pipes；393 third pipes；
4 containers.
Specific embodiment
Exemplary embodiments of the present invention are described with reference to the accompanying drawings.It should be appreciated that these specific descriptions are only used for
How teaching those skilled in the art implement the present invention, rather than all feasible modes that exhaustion is of the invention, without in
It limits the scope of the invention.
"left", "right" that the present invention refers to, "up" and "down" are all based on for the visual angle of Fig. 1.
The specific embodiment of optical detection apparatus according to the present invention is described in detail below with reference to Fig. 1 to Fig. 4.
In the present embodiment, as shown in Figure 1, optical detection apparatus includes detection body 1, light source mechanism 2 and detection group
Part 3.
In the present embodiment, as shown in Figures 2 and 3, detection body 1 is whole substantially in a rectangular parallelepiped shape, and detection body 1 is wrapped
Include light incidence channel 11, light propagation channel 12, transmitted light receiving channel 13 and receiving hole 14.
In the present embodiment, as shown in figure 3, light incidence channel 11 extends from the left side of detection body 1 towards right side
Setting.Wherein, the cross section of light incidence channel 11 is circle.
In the present embodiment, as shown in figure 3, light propagation channel 12 includes bellmouth 121, cylindrical hole 122 and limited wall
Face 123 (limiting section).Wherein, cylindrical hole 122 is extended from the right side of detection body 1 towards left side, the right side of bellmouth 121
End is connected with cylindrical hole 122, and the left end of bellmouth 121 is connected to receiving hole 14,122 connecting place shape of bellmouth 121 and cylindrical hole
At limit wall surface 123.
In the present embodiment, bellmouth 121 is formed as more bigger close to 122 aperture of cylindrical hole.In this way, being reasonably arranged
The taper of bellmouth 121 can make the scattering light of angle of scattering within a predetermined range enter light propagation channel 12.
In the present embodiment, the cross section of bellmouth 121 and cylindrical hole 122 is circle.
In the present embodiment, as shown in figure 3, the wall portion of the restriction cylindrical hole 122 of detection body 1 opens up transmission light-receiving
Channel 13, transmitted light receiving channel 13 is perpendicular to cylindrical hole 122.Wherein, the cross section of transmitted light receiving channel 13 is circle.
In the present embodiment, as shown in figure 3, receiving hole 14 be set to light incidence channel 11 and light propagation channel 12 it
Between, and be connected to light incidence channel 11 and light propagation channel 12.Wherein, the cross section of receiving hole 14 is rectangle, receiving hole 14
For accommodating the container 4 for installing sample to be detected.In the present embodiment, container 4 is cuvette.
In the present embodiment, as shown in figure 3, light source mechanism 2 includes laser 21 and mounting base 22.21 part of laser
Ground is inserted into light incidence channel 11, and the part of laser 21 being located at outside light incidence channel 11 is connect with mounting base 22, laser
21 are installed on detection body 1 by mounting base 22.
In the present embodiment, as shown in figure 3, there is adjustment gap 211 between laser 21 and light incidence channel 11.This
Sample can finely tune the direction of the optical axis of laser 21 as needed, and then obtain and preferably receive luminous intensity.
In the present embodiment, as shown in Figure 3 and Figure 4, detection components 3 include the first convex lens 31, the first planar lens
32, reflecting mirror 33, the second planar lens 34, the second convex lens 35, positioning mounting plate 36,37 (the first light of the first opto-electronic receiver plate
Electric reception device), the second opto-electronic receiver plate 38 (the second photoelectric receiving arrangement) and pipe 39.
In the present embodiment, as shown in figure 3, the first convex lens 31, the first planar lens 32, reflecting mirror 33, second are flat
Face lens 34 and the second convex lens 35 are set in turn in left-right direction in cylindrical hole 122, positioning mounting plate 36 and the first photoelectricity
Receiver board 37 is set at the right end of detection body 1, and the second opto-electronic receiver plate 38 is set to deviating from for transmitted light receiving channel 13
At one end of cylindrical hole 122.Wherein, the first convex lens 31, the first planar lens 32, the second planar lens 34 and the second convex lens
35 are vertically arranged, and reflecting mirror 33 is obliquely extended relative to the first planar lens 32.
In the present embodiment, reflecting mirror 33 in left-right direction be projected as circle.
In the present embodiment, as shown in Figure 3 and Figure 4, the first planar lens 32 is provided with the first mounting hole 321, and second
Planar lens 34 is provided with the second mounting hole 341.Wherein, the first mounting hole 321 is opened in the first planar lens in left-right direction
32 center, the second mounting hole 341 are opened in the center of the second planar lens 34 in left-right direction.
In the present embodiment, as shown in Figure 3 and Figure 4, the hole of the upper end of reflecting mirror 33 and the top of the first mounting hole 321
Wall bonding, the lower end of reflecting mirror 33 are Nian Jie with the hole wall of lower part of the second mounting hole 341.In this way, the first planar lens 32, reflection
Mirror 33 and 34 shape of the second planar lens are integral, and are formed between the periphery of reflecting mirror 33 and the inner wall of pipe 39 for being in ring
The space that the scattering light light beam of shape passes through.
In the present embodiment, as shown in Figure 3 and Figure 4, pipe 39 includes the first pipe 391, the second pipe 392 and third
Pipe 393.Wherein, the first pipe 391 is set between the first convex lens 31 and the first planar lens 32, and the second pipe 392 is set
It is placed between the second planar lens 34 and the second convex lens 35, third pipe 393 is set to the second convex lens 35 and location and installation
Between plate 36.
In the present embodiment, as shown in Figure 3 and Figure 4, positioning mounting plate 36 includes baseplate part 361 and cylindrical part 362.Its
In, the cross section of baseplate part 361 is rectangle, and from the left side of baseplate part 361, protrusion extends cylindrical part 362 to the left.
In the present embodiment, as shown in Figure 3 and Figure 4, baseplate part 361 is fixedly installed in detection body 1 by fastener
Right part, cylindrical part 362 be inserted into light propagation channel 12 in abut third pipe 393.
In the present embodiment, as shown in Figure 3 and Figure 4, the first opto-electronic receiver plate 37 is provided with the first photocell 371, uses
Light is scattered in receiving to be detected.
In the present embodiment, as shown in Figure 3 and Figure 4, the second opto-electronic receiver plate 38 is provided with the second photocell 381, uses
In reception transmitted light to be detected.
In the present embodiment, as shown in Figure 3 and Figure 4, the first opto-electronic receiver plate 37 passes through together with positioning mounting plate 36
Fastener is fixedly installed in the right part of detection body 1, so that the left end of cylindrical part 362 abuts third pipe 393, third pipe
393 left end abuts the second convex lens 35, and the left end of the second pipe 392 abuts the second planar lens 34, a left side for the first pipe 391
End abuts the first convex lens 31, and the first convex lens 31 abuts limit wall surface 123.
The course of work of optical detection apparatus according to the present invention is briefly described below.
In the present embodiment, when the container 4 for installing sample to be detected is set in receiving hole 14, laser 21 is issued
Laser, laser irradiation to container 4 and pass through container 4 in sample after formed transmitted light and scattering light enter light propagation channel 12.
Transmitted light is reflected into transmission light-receiving by reflecting mirror 33 after the first convex lens 31, the first mounting hole 321 and leads to
Road 13, and finally handled by 38 reception of the second opto-electronic receiver plate.
After scattering light through the first convex lens 31, the first planar lens 32, the second planar lens 34 and the second convex lens 35
It is handled by 37 reception of the first opto-electronic receiver plate.
By using above-mentioned technical proposal, optical detection apparatus according to the present invention is at least had the advantages that
(1) in optical detection apparatus of the invention, transmitted light detection function and scattering light detection function are integrated in one
Body can be improved detection efficiency.
(2) in optical detection apparatus of the invention, pass through the first convex lens of setting, reflecting mirror and the second convex lens, energy
Enough sizes by the way that reflecting mirror is reasonably arranged, the scattering light of angle required for adjusting transmitted light beam intensity and obtaining, into
And improve detection effect.
(3) in optical detection apparatus of the invention, wall surface, pipe and positioning mounting plate are limited by setting, so that the
One convex lens, the first planar lens, reflecting mirror, the second planar lens and the second convex lens are fixed by way of abutting, into
And different size of reflecting mirror can be replaced easily as needed to adjust the strong of the second received transmitted light of opto-electronic receiver plate
Degree improves the sensitivity and resolution ratio of transmission light detection；Meanwhile it also can easily replace the convex lens and difference of different curvature
The pipe of diameter come angle required for adjusting to obtain scattering light (correspondingly need to adjust while adjustment convex lens mirror curvature every
The length of pipe), improve the detection effect of scattering light detection.
(4) in optical detection apparatus of the invention, the wall surface of the restriction light incidence channel of laser and detection body it
Between there is adjustment gap, make it possible to finely tune the optical axis of laser, improve detection effect.
Above specific embodiment elaborates technical solution of the present invention, but also needs to add explanation
:
(1) of the invention although the cross section for illustrating bellmouth and cylindrical hole in the above-described embodiment is circle
Without being limited thereto, the cross section of bellmouth and cylindrical hole may be polygon.
(2) although illustrating that light source mechanism includes laser in the above-described embodiment, but the invention is not restricted to this,
Laser can be substituted using LED light source.
(3) although be inserted into light incidence channel with illustrating laser component in the above-described embodiment, the present invention is not
It is limited to this, laser can also all be set to the outside of light incidence channel.
(4) although illustrating transmitted light receiving channel in the above-described embodiment perpendicular to cylindrical hole, and its cross section is
Circle, but the invention is not restricted to this, and transmitted light receiving channel can also obliquely extend relative to cylindrical hole, transmits light-receiving
The cross section in channel may be polygon.
(5) although illustrating that reflecting mirror is projected as circle, this hair along left and right directions in the above-described embodiment
Bright without being limited thereto, reflecting mirror may be polygon along the projection of left and right directions.
(6) although illustrating that the first opto-electronic receiver plate is installed on detection originally by positioning mounting plate in the above-described embodiment
The right end of body, and positioning mounting plate pushes against third pipe, but the invention is not restricted to this, it can also be not provided with positioning mounting plate,
So that the first opto-electronic receiver plate directly pushes against third pipe.
In addition, the specific protein analyzer includes above-mentioned optics the present invention also provides a kind of specific protein analyzer
Detection device.
It is appreciated that the specific protein analyzer with the optical detection apparatus has same beneficial effect.
Claims (10)
1. a kind of optical detection apparatus, which is characterized in that the optical detection apparatus includes detection body, light source mechanism and detection
Component,
The detection body is provided with light incidence channel, light propagation channel, transmitted light receiving channel and receiving hole,
The receiving hole is set between the smooth incidence channel and the light propagation channel, installs sample to be detected for accommodating
Container,
The transmitted light receiving channel is opened in the wall portion in the restriction light propagation channel of the detection body,
The light source mechanism is installed on the detection body, and when the container for installing sample to be detected is set to the receiving
When in hole, the light that the light source mechanism issues can expose to the container, generated transmission by the smooth incidence channel
Light and scattering light enter the light propagation channel,
The detection components include that the first convex lens, reflecting mirror, the second convex lens, the first photoelectric receiving arrangement and the second photoelectricity connect
Receiving apparatus,
First convex lens, the reflecting mirror and second convex lens along the light propagation channel extending direction successively
It is set in the light propagation channel,
The space passed through for the scattering light is formed between the periphery of the reflecting mirror and the wall portion,
First photoelectric receiving arrangement is set to the one end opposite with the receiving hole in the light propagation channel, for receiving
The scattering light of first convex lens and second convex lens is sequentially passed through,
Second photoelectric receiving arrangement is set to one end away from the light propagation channel of the transmitted light receiving channel, uses
In receiving across first convex lens and the transmitted light that is reflected by the reflecting mirror.
2. optical detection apparatus according to claim 1, which is characterized in that the detection components further include that the first plane is saturating
Mirror, the second planar lens and pipe,
First planar lens and second planar lens are spaced apart along the extending direction to be arranged,
The reflecting mirror is separately connected with first planar lens and second planar lens, and the reflecting mirror is described
On extending direction between first planar lens and second planar lens,
The pipe includes the first pipe, the second pipe and third pipe,
First pipe is set between first convex lens and first planar lens,
Second pipe is set between second planar lens and second convex lens,
The third pipe is set between second convex lens and first photoelectric receiving arrangement,
The light propagation channel is provided with limiting section,
First photoelectric receiving arrangement pushes against the third pipe, so that the third pipe abuts second convex lens,
Second pipe abuts second planar lens, and first pipe abuts first convex lens, first convex lens
Mirror abuts the limiting section.
3. optical detection apparatus according to claim 2, which is characterized in that the light propagation channel includes interconnected
Bellmouth and cylindrical hole,
One end of the bellmouth is connected to the receiving hole, and the other end and the cylindrical hole connectivity part form the limiting section,
The bellmouth is formed as more bigger close to the cylindrical hole aperture.
4. optical detection apparatus according to claim 2, which is characterized in that first installation of the first planar lens setting
The second mounting hole, the hole wall and described second of the reflecting mirror and first mounting hole is arranged in hole, second planar lens
The hole wall of mounting hole is separately connected.
5. optical detection apparatus according to claim 2, which is characterized in that the detection components further include location and installation
Plate,
One end of the positioning mounting plate abuts the third pipe, and the other end is abutted with first photoelectric receiving arrangement.
6. optical detection apparatus according to claim 1, which is characterized in that it is incident that the light source mechanism is inserted in the light
Channel, and there is adjustment gap between the wall surface of the restriction smooth incidence channel of the light source mechanism and the detection body.
7. optical detection apparatus according to claim 1, which is characterized in that the transmitted light receiving channel is perpendicular to described
It is arranged through the wall portion to light propagation channel.
8. optical detection apparatus according to claim 1, which is characterized in that the light source mechanism includes laser and installation
Seat, the laser are installed on the detection body by the mounting base.
9. optical detection apparatus according to claim 1, which is characterized in that the cross section of the receiving hole is rectangle, and/
Or the reflecting mirror is projected as circle along the extending direction.
10. a kind of specific protein analyzer, which is characterized in that the specific protein analyzer includes any in claim 1 to 9
Optical detection apparatus described in.
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| CN201910022037.7A CN109738393B (en) | 2019-01-10 | 2019-01-10 | Optical detection device and specific protein analyzer |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910022037.7A CN109738393B (en) | 2019-01-10 | 2019-01-10 | Optical detection device and specific protein analyzer |
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Cited By (1)
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| Publication number | Publication date |
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| CN109738393B (en) | 2021-03-05 |
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