CN102499647A - Multi-mode low-coherence scattering spectrometer - Google Patents
Multi-mode low-coherence scattering spectrometer Download PDFInfo
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- CN102499647A CN102499647A CN2011103586459A CN201110358645A CN102499647A CN 102499647 A CN102499647 A CN 102499647A CN 2011103586459 A CN2011103586459 A CN 2011103586459A CN 201110358645 A CN201110358645 A CN 201110358645A CN 102499647 A CN102499647 A CN 102499647A
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Abstract
The invention discloses a multi-mode low-coherence scattering spectrometer, which is characterized by being provided with a white light source, a collimating light path and a polarizer. White light emitted from the white light source irradiates on a reflector after being collimated and polarized, reflected light from the reflector irradiates into an observed sample after transmitting a spectroscope, scattered light of the observed sample is reflected to a polarization analyzer through the spectroscope, light ray permeating the polarization analyzer is absorbed by a duct scope, a spectrometer is mounted along the focal length of the duct scope, a first CCD (charge coupled device) camera is connected to the spectrometer, a movable reflector is arranged between the duct scope and the spectrometer and used for reflecting light ray projected from the duct scope into a second CCD camera. The multi-mode low-coherence scattering spectrometer has the advantages that tissues can be detected noninvasively, variation of cell features caused by dyeing is avoided, structural information of shallow tissue and deep tissue can be acquired simultaneously by the polarization gating technology, spectrum, scattering angle, azimuth angle and polarization character of the tissues can be acquired simultaneously by means of wideband incidence of white light, and structural composition of the tissues can be described quantitatively.
Description
Technical field
The present invention relates to a kind of spectrogrph, the low coherent scatter spectra appearance of multi-mode in particular for histiocyte is measured belongs to optical image technology.
Background technology
Because traditional histopathology detects and often need fix tissue, dyeing is then through the histiocytic morphosis of microscopic examination.The a series of processing of process possibly cause the structure or the characteristic change of tissue, cause the reliability of testing result not high enough.Simultaneously, also lack quantitative parameter in the prior art and describe the variation of organizing micrometer structure.Therefore, obtain noinvasive biological tissue's degree of depth chromatography inferior diffusion yardstick optical scattering and structure that absorption characteristic is come research organization with form significant.
Summary of the invention
The objective of the invention is to develop a kind of low coherent scatter spectra appearance of multi-mode of inferior diffusion yardstick of degree of depth chromatography, but noinvasive directly tissue is measured, obtain the quantitative scatter and the absorption parameter of tissue, thereby describe the micrometer structure of tissue.
For achieving the above object, the scheme that the present invention adopted is following:
A kind of multi-mode is hanged down the coherent scatter spectra appearance; Be provided with white light source; The white light that this white light source sends is realized collimation through collimated light path; In collimated light path, be provided with polaroid; Linearly polarized photon after rising partially through collimation is incident on the illuminator; The reflected light of this illuminator sees through spectroscope and incides in the observation sample; The scattered light of this observation sample reflexes in the checking bias slice through said spectroscope; Pass the light of checking bias slice and managed the mirror absorption; On the focal length of Guan Jing, spectrogrph is installed; On this spectrogrph, be connected with a CCD camera; The one CCD camera is used to generate the scattered light intensity graph of a relation of wavelength
and angle of scattering
under the particular azimuth
; Also be provided with removable reflecting mirror in the light path between said Guan Jingyu spectrogrph; The light that this removable reflecting mirror projects Guan Jing reflexes in the 2nd CCD camera, and the 2nd CCD camera is used to generate the scattered light intensity graph of a relation at angle of scattering
and azimuth
.
Multi-mode proposed by the invention is hanged down the coherent scatter spectra appearance, can measure angle and the azimuth and the polarization scattering characteristic of spectral distribution and particular particles of the backscattered light of tissue simultaneously.Through measurement to scattered signal; Analyze its spectrum
; Angle of scattering
; Azimuth
and polarization characteristic, thus the micrometer structure of organizing is described.The structural information of tissue is provided the scattered signal noinvasive, need not have carried out any processing, can detect the slight change of organizational structure.
Utilize the white light signal after the polaroid collimation to rise partially, linearly polarized photon is incided on the sample, can write down the same polarization signal and the orthogonal polarization signals of back-reflections through the rotation checking bias slice respectively.Because the depolarizing property of multiple scattering, from obtaining the difference polarization signal with deducting orthogonal polarization signals the polarization signal, the difference polarization signal mainly comes from the scattering,single of organizing the top layer.The scattering,single signal that is obtained is sensitive especially to the characteristic of textura epidermoidea, and orthogonal polarization signals is sensitive with composition to the structure of degree of depth tissue, therefore, utilizes the polarization gate technique can realize the selection of investigation depth.
System adopts white light source to realize wide range incident; Corresponding different investigation depths; The one CCD camera can generate the scattered light intensity graph of a relation of wavelength
and angle of scattering
under the particular azimuth
, and the 2nd CCD camera can generate the scattered light intensity graph of a relation at angle of scattering
and azimuth
.Through obtaining information being carried out the blood content that analyzing and processing can be obtained tissue, fractal dimension and anisotropy factor, thereby the quantitative micrometer structure characteristic of describing out tissue.
For the white light that white light source is sent is adjusted collimation, said collimated light path is rearranged by condenser lens, first lens, first diaphragm, second lens and second diaphragm successively, between said second lens and second diaphragm, said polaroid is set.
The angle of divergence of the incident ray behind said collimated light path collimation is 0.3 °～0.5 °, and diameter is low to moderate 2mm.
For fear of the specular light of tissue surface, when the reflected light of said illuminator incided in the observation sample through spectroscope, the incident illumination of observation sample became 15 ° of angles with the normal incidence direction.
In order to observe convenient, also be provided with object stage, said observation sample is placed on the object stage.
Said white light source is an xenon source, and the white light intensity of sending is big, and degree of coherence is little.
Remarkable result of the present invention is: light channel structure is simple, and control is convenient, and system can measure organizational structure on noinvasive ground; Dye without pair cell; Avoid causing because of dyeing the variation of cell characteristic, system adopts the polarization gate technique to realize the degree of depth laminar analysis measurement of tissue, can obtain the structural information of shallow layer tissue and degree of depth tissue simultaneously; Adopt the incident of white light wide range; Can obtain spectrum, angle of scattering, azimuth and the polarization characteristic of tissue simultaneously,, can describe the structure of tissue quantitatively and form through analysis to scattered signal.
Description of drawings
Fig. 1 is system design figure of the present invention;
Reference numeral:
1 white light source, 2 condenser lenss, 3 first lens, 4 first diaphragms, 5 second lens, 6 polaroids; 7 second diaphragms, 8 reflecting mirrors, 9 spectroscopes, 10 object stages, 11 checking bias slices; 12 pipe mirrors, 13 removable reflecting mirrors, 14 the one CCD cameras, 15 spectrogrphs, 16 the 2nd CCD cameras.
The specific embodiment
Below in conjunction with accompanying drawing and specific embodiment the present invention is done further explain.
As shown in Figure 1, a kind of multi-mode is hanged down the coherent scatter spectra appearance, is provided with white light source 1, and this white light source 1 adopts xenon source, and the white light intensity of sending is big, and degree of coherence is little.The white light that white light source 1 sends is realized collimation through collimated light path; This collimated light path is rearranged by condenser lens 2, first lens 3, first diaphragm 4, second lens 5 and second diaphragm 7 successively; The angle of divergence of incident ray is 0.3 °～0.5 ° behind the collimation, 0.4 ° of optimum, and diameter is low to moderate 2mm.
Between said second lens 5 and second diaphragm 7, be provided with polaroid 6; Linearly polarized photon after rising partially through collimation is incident on the illuminator 8; The reflected light of this illuminator 8 sees through spectroscope 9 and incides in the observation sample, and observation sample is placed on the object stage 10, for fear of the specular light of tissue surface; When the reflected light of illuminator 8 incided in the observation sample through spectroscope 9, the incident illumination of observation sample became 15 ° of angles with the normal incidence direction.
The scattered light of observation sample reflexes in the checking bias slice 11 through said spectroscope 9, passes the light of checking bias slice 11 and is managed mirror 12 absorptions, checking bias slice 11 is placed on pipe mirror 12 can measures the same polarization signal and the orthogonal polarization signals of tissue scatter before independently.On the focal length of pipe mirror 12, spectrogrph 15 is installed; The entrance slit of spectrogrph 15 aligns with the focal length of pipe mirror 12; Therefore; Scattered beam with same angle of scattering
and azimuth
will focus on the same point of slit; Spectrogrph 15 expands to light on the vertical direction of slit; On this spectrogrph 15, be connected with a CCD camera 16, can generate the scattered light intensity graph of a relation of wavelength
and angle of scattering
under the particular azimuth
through a CCD camera 16.
Also be provided with removable reflecting mirror 13 in the light path between said pipe mirror 12 and spectrogrph 15; This removable reflecting mirror 13 can reflex to the light that pipe mirror 12 transmits in the 2nd CCD camera 14, can generate the scattered light intensity graph of a relation at angle of scattering
and azimuth
through the 2nd CCD camera 14.
Analysis of the first CCD camera 16 generates a wavelength
and the scattering angle
diagram of the scattered light intensity.Get angle of scattering
and ask average to the spectral signal between+2 °; Obtain the mean intensity
of scattered light; According to
; Wherein
represents the absorption spectrum of reduced hemoglobin;
represents the absorption spectrum of HbO2 Oxyhemoglobin, and the reduced blood that can obtain organizing through least square fitting contains coefficient of discharge
and oxygenated blood contains coefficient of discharge
.The angle of scattering signal
of supposing to get wavelength
carries out Fourier transform; Get the slope of signal behind the Fourier transform, just can obtain the fractal dimension of tissue.
The second generation of CCD cameras 14 scattering angle
and azimuth
diagram of the scattered light intensity.The collection of illustrative plates of naming a person for a particular job from the center is divided into a series of concentric circular; Light intensity in the concentric circular is superposeed; Can obtain the signal
relevant with angle of scattering
;
signal to
carries out normalization to be handled and to acquire
, can inverting according to
calculates the anisotropy factor g of tissue.
Through organizing blood content, fractal dimension and anisotropy factor g get final product the micrometer structure of quantitative description tissue, reach the object of the invention.Because this technology is non-invasive, can be used for the measurement of biological tissue in the body, have very big practical value.
Claims (6)
1. a multi-mode is hanged down the coherent scatter spectra appearance; It is characterized in that: be provided with white light source (1); The white light that this white light source (1) sends is realized collimation through collimated light path; In collimated light path, be provided with polaroid (6); Linearly polarized photon after rising partially through collimation is incident on the illuminator (8); The reflected light of this illuminator (8) sees through spectroscope (9) and incides in the observation sample; The scattered light of this observation sample reflexes in the checking bias slice (11) through said spectroscope (9); The light that passes checking bias slice (11) is absorbed by Guan Jing (12); Spectrogrph (15) is installed on the focal length of Guan Jing (12); On this spectrogrph (15), be connected with a CCD camera (16); The one CCD camera (16) is used to generate the scattered light intensity graph of a relation of wavelength
and angle of scattering
under the particular azimuth
; Also be provided with removable reflecting mirror (13) in the light path between said Guan Jing (12) and spectrogrph (15); The light that this removable reflecting mirror (13) transmits Guan Jing (12) reflexes in the 2nd CCD camera (14), and the 2nd CCD camera is used to generate the scattered light intensity graph of a relation at angle of scattering
and azimuth
.
2. a kind of multi-mode according to claim 1 is hanged down the coherent scatter spectra appearance; It is characterized in that: said collimated light path is rearranged by condenser lens (2), first lens (3), first diaphragm (4), second lens (5) and second diaphragm (7) successively, between said second lens (5) and second diaphragm (7), said polaroid (6) is set.
3. a kind of multi-mode according to claim 2 is hanged down the coherent scatter spectra appearance, it is characterized in that: the angle of divergence of the incident ray behind said collimated light path collimation is 0.3 °～0.5 °, and diameter is low to moderate 2mm.
4. a kind of multi-mode according to claim 1 is hanged down the coherent scatter spectra appearance, it is characterized in that: when the reflected light of said illuminator (8) incided in the observation sample through spectroscope (9), the incident illumination of observation sample became 15 ° of angles with the normal incidence direction.
5. a kind of multi-mode according to claim 1 is hanged down the coherent scatter spectra appearance, it is characterized in that: be provided with object stage (10), said observation sample is placed on the object stage (10).
6. a kind of multi-mode according to claim 1 is hanged down the coherent scatter spectra appearance, it is characterized in that: said white light source (1) is an xenon source.
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| CN102499647B CN102499647B (en) | 2013-11-27 |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108344694A (en) * | 2018-02-26 | 2018-07-31 | 长光禹辰信息技术与装备(青岛)有限公司 | A kind of multispectral instrument of multi-mode multiplexing |
| CN109738393A (en) * | 2019-01-10 | 2019-05-10 | 上海奥普生物医药有限公司 | Optical detection apparatus and specific protein analyzer |
| CN113591723A (en) * | 2020-09-08 | 2021-11-02 | 神盾股份有限公司 | Biometric sensing device |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0458601A1 (en) * | 1990-05-22 | 1991-11-27 | Research Development Corporation Of Japan | Method of and apparatus for measuring spectral absorption in opaque specimens and method of and apparatus for measuring microscopic absorption distribution |
| CN101000300A (en) * | 2006-12-30 | 2007-07-18 | 清华大学深圳研究生院 | Linear polarization optical imaging method and device |
| CN101548153A (en) * | 2006-05-12 | 2009-09-30 | 西北大学 | Systems, methods, and apparatuses of low-coherence enhanced backscattering spectroscopy |
-
2011
- 2011-11-14 CN CN2011103586459A patent/CN102499647B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0458601A1 (en) * | 1990-05-22 | 1991-11-27 | Research Development Corporation Of Japan | Method of and apparatus for measuring spectral absorption in opaque specimens and method of and apparatus for measuring microscopic absorption distribution |
| CN101548153A (en) * | 2006-05-12 | 2009-09-30 | 西北大学 | Systems, methods, and apparatuses of low-coherence enhanced backscattering spectroscopy |
| CN101000300A (en) * | 2006-12-30 | 2007-07-18 | 清华大学深圳研究生院 | Linear polarization optical imaging method and device |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108344694A (en) * | 2018-02-26 | 2018-07-31 | 长光禹辰信息技术与装备(青岛)有限公司 | A kind of multispectral instrument of multi-mode multiplexing |
| CN109738393A (en) * | 2019-01-10 | 2019-05-10 | 上海奥普生物医药有限公司 | Optical detection apparatus and specific protein analyzer |
| CN113591723A (en) * | 2020-09-08 | 2021-11-02 | 神盾股份有限公司 | Biometric sensing device |
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| CN102499647B (en) | 2013-11-27 |
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