CN212301289U - Integrated fluorescence detection device - Google Patents
Integrated fluorescence detection device Download PDFInfo
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- CN212301289U CN212301289U CN202021532071.3U CN202021532071U CN212301289U CN 212301289 U CN212301289 U CN 212301289U CN 202021532071 U CN202021532071 U CN 202021532071U CN 212301289 U CN212301289 U CN 212301289U
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Abstract
The utility model provides an integrated fluorescence detection device, which comprises a double-channel light path structure and a box body, wherein the box body is provided with a box body cover plate and a box body bottom plate, the box body cover plate is of a cavity structure, a movable cover is arranged at the upper end of the box body bottom plate, and one side surface is provided with an open pore; the double-channel light path structure is fixedly installed at the bottom of the box body cavity structure and is provided with a light path support, a light path support cover plate, an optical component and a focusing lens, one end of the light path support is connected with the focusing lens through threads, the other end of the light path support is completely installed in the box body, the light path support cover plate is installed at the top of the light path support through a plurality of groups of installation holes, and the optical component is fixedly installed in the light path support. The device adopts the principle of confocal light path, integrates the double-channel excitation detection light path into a small-volume box body, and is used independently or integrated into a related instrument as a functional module; by adopting a dual-channel design and presetting a plurality of working modes, the time-sharing multiplexing of two-wavelength excitation detection or single-channel excitation dual-channel detection can be realized.
Description
Technical Field
The utility model belongs to the technical field of the fluorescence detection, belong to optical detection, in particular to integration fluorescence detection device.
Background
Fluorescence is used as an important optical signal and has wide application in the fields of biology, chemistry, medical treatment and the like, most common fluorescence detection devices in the market at present adopt a distributed or open structure, and an excitation light source and a detector cannot be well integrated into a closed box body, so that the device is large in size and complex in structure, and is difficult to be used independently or integrated into related instruments as a functional module. In addition, the distributed structure also reduces the stability of the device, and influences the accuracy of the detection result.
Disclosure of Invention
To above problem, the utility model provides an integration fluorescence detection device, the device adopts the principle of confocal light path, arouse the binary channels and detect that the light path is integrated to a volume less than or equal to 60X 40X 20 mm's box body, set up only a focusing lens of light path part and outside UNICOM, the control circuit part is only power and communication interface and outside UNICOM, communication interface through customized communication protocol and reservation, can control the device exclusive use or as the function module integration to relevant instrument in, the device adopts the binary channels design, predetermine multiple mode, can realize that two kinds of wavelength arouse and detect time sharing multiplex or single pass arouse the double-circuit and detect, adapt to multiple application scene.
The integrated fluorescence detection device comprises a double-channel light path structure and a box body, wherein the box body is provided with a box body cover plate and a box body bottom plate, the box body cover plate is of a cavity structure, the movable cover is arranged at the upper end of the box body bottom plate, and one side surface of the movable cover is provided with an opening; binary channels light path structure fixed mounting is provided with light path support, light path support apron, optical component, focusing lens in the bottom of box body cavity structure, wherein a light path support tip passes through threaded connection focusing lens, and the other end is installed completely in the box body, light path support apron is installed at light path support top through the multiunit mounting hole, optical component fixed mounting is in the light path support.
As the improvement, still include the circuit board, set up in binary channels light path structure bottom, through fastener detachably with binary channels light path structure and circuit board fixed mounting in the bottom of box body cavity structure.
As an improvement, the circuit board comprises a power management unit, a light source driving unit, a signal amplification and filtering unit, an analog-to-digital conversion unit, a communication interface unit and a microcontroller core unit; the power management unit is used for providing power supply power, the input end of the power management unit is communicated with an external power supply device through a power interface, and the output end of the power management unit is electrically connected with the power utilization unit in the circuit board; the light source driving units are divided into two paths, are used for driving the excitation light source and are electrically connected with the excitation light source bottom plate; the signal amplification and filtering unit is positioned on the bottom plate of the detector, is electrically connected with the analog-to-digital conversion unit and is used for converting the fluorescence signal into a digital electric signal which can be processed by the microcontroller; the communication interface unit adopts RS232, RS485, I2C or SPI communication protocol; the microcontroller adopts a mainstream singlechip.
As an improvement, the optical component comprises a first dichroic mirror, a second dichroic mirror, a third dichroic mirror and a fourth dichroic mirror, and a main light path axis of the optical component is set along the central direction of the focusing lens, wherein the first dichroic mirror, the second dichroic mirror, the third dichroic mirror and the fourth dichroic mirror are sequentially arranged in the light path support from near to far away at a certain distance from the focusing lens; the first dichroic mirror and the second dichroic mirror are arranged in parallel and are respectively and fixedly installed at an included angle of 45 degrees with the axis of the main light path; and the third dichroic mirror and the fourth dichroic mirror are arranged in parallel and are fixedly installed at an included angle of 135 degrees with the axis of the main light path independently.
As an improvement, two sides of the main optical path axis in the optical path support are respectively and independently provided with a group of channels, and an optical component in each group of channels comprises an excitation light source, an excitation lens, a detection optical filter, a detector, a detection lens and an excitation optical filter; one side of the first dichroic mirror or the third dichroic mirror is sequentially provided with an excitation optical filter, an excitation lens and an excitation light source from near to far along the axis of the main light path; one side of the second dichroic mirror or the fourth dichroic mirror is sequentially provided with a detection optical filter, a detection lens and a detector from near to far along the axis of the main light path.
As an improvement, the excitation light source is fixedly arranged on the focal length of the laser lens, the detector is fixedly arranged on the focal length of the detection lens, and the detection lens and the laser lens are fixedly arranged inside the lens sleeve and are respectively set to be a biconvex lens or a plano-convex lens.
As a refinement, the focusing lens is configured as a biconvex lens or a plano-convex lens.
As an improvement, the excitation light source is set as any one of a light-emitting diode, a laser and a halogen tungsten lamp, and the detector is selected as a photodiode or a photomultiplier.
Meanwhile, the integrated fluorescence detection device is also applied to time-sharing multiplexing of two-wavelength excitation detection or single-path excitation double-path detection.
Has the advantages that: the utility model provides an integration fluorescence detection device compares with current detection device, has following advantage:
(1) the utility model adopts the integrated design, integrates all optical components of the double-channel fluorescence detection in a closed optical path structure, and simultaneously encapsulates the optical path structure and the control circuit in a closed metal box body, thereby greatly improving the stability of the device and effectively shielding external stray light and electromagnetic noise interference;
(2) the whole device has the advantages that the volume of the shell is less than 60 multiplied by 40 multiplied by 20mm, the device is small and portable, a power supply and a communication interface are reserved in a control circuit, and the device can be used independently and can also be integrated into related instruments as a functional module;
(3) the dual-channel design is adopted, multiple working modes are preset, time-sharing multiplexing of two-wavelength excitation detection can be realized, and single-channel excitation and double-channel detection can be realized, so that the device is suitable for multiple application scenes;
(4) by adding the reflector behind the focusing lens, the direction of the focusing lens can be conveniently changed to be upward or downward, and the top excitation detection or the bottom excitation detection can be conveniently applied.
Drawings
Fig. 1 is an overall structure view of the outer side surface of the device of the present invention.
Fig. 2 is a view showing the internal structure of the case of the device of the present invention.
Fig. 3 is a diagram of the dual channel optical path structure and optical assembly position of the device of the present invention.
Fig. 4 is a schematic diagram of the dual-channel optical path principle of the device of the present invention.
In the drawings: 1. a dual channel optical path structure; 101. an optical path support; 102. a focusing lens; 103. a first dichroic mirror; 104. A second dichroic mirror; 105. a third dichroic mirror; 106. a fourth dichroic mirror; a first excitation filter 107, a second excitation filter 108, a first detection filter 109, and a second detection filter 110; 111. a first excitation lens; 112. A second excitation lens; 113. a first detection lens; 114. a second detection lens; 115. an excitation light source baseplate; 116. A detector backplane; 117. a first detector; 118. a second detector; 119. mounting holes; 120. a light path bracket cover plate; 2. a circuit board; 3. a box body; 301. a box body cover plate; 302. a box body bottom plate.
Detailed Description
An integrated fluorescence detection device comprises a dual-channel light path structure 1 and a box body 3, wherein the box body 3 is provided with a box body cover plate 301 and a box body bottom plate 302, the box body cover plate 301 is of a cavity structure, the movable cover is arranged at the upper end of the box body bottom plate (302), and one side surface of the movable cover is provided with an opening; binary channels light path structure 1 fixed mounting is provided with light path support 101, light path support apron 120, optical component, focusing lens 102 in the bottom of 3 cavity structures of box body, wherein a light path support 101 tip is through threaded connection focusing lens 102, and the other end is installed completely in box body 3, light path support apron 120 passes through multiunit mounting hole 119 and installs at light path support 101 top, optical component fixed mounting is in light path support 101.
Still include circuit board 2, set up in 1 bottoms of binary channels light path structure, through fastener detachably with binary channels light path structure 1 and circuit board 2 fixed mounting in 3 cavity structures's of box body bottom.
The circuit board 2 comprises a power supply management unit, a light source driving unit, a signal amplification and filtering unit, an analog-to-digital conversion unit, a communication interface unit and a microcontroller core unit; the power management unit is used for providing power supply power, the input end of the power management unit is communicated with an external power supply device through a power interface, and the output end of the power management unit is electrically connected with the power utilization unit in the circuit board 2; the light source driving units are provided with two paths for driving the excitation light source and are electrically connected with the excitation light source bottom plate 115; the signal amplifying and filtering unit is positioned on the detector bottom plate 116, is electrically connected with the analog-to-digital conversion unit, and is used for converting the fluorescent signal into a digital electric signal which can be processed by the microcontroller; the communication interface unit can adopt RS232, RS485, I2C or SPI communication protocols; the microcontroller can adopt various mainstream singlechips including but not limited to C8051F series, STM32 series, MSP430 series and the like.
The optical component comprises a first dichroic mirror 103, a second dichroic mirror 104, a third dichroic mirror 105 and a fourth dichroic mirror 106, and a main light path axis which is the optical component along the central direction of the focusing lens 102 is set, wherein the first dichroic mirror 103, the second dichroic mirror 104, the third dichroic mirror 105 and the fourth dichroic mirror 106 are sequentially arranged in the light path support 101 from near to far away from the focusing lens 102; the first dichroic mirror 103 and the second dichroic mirror 104 are arranged in parallel and are respectively and fixedly installed at an included angle of 45 degrees with the axis of the main light path; the third dichroic mirror 105 and the fourth dichroic mirror 106 are arranged in parallel, and are respectively and fixedly installed at an included angle of 135 degrees with the axis of the main light path.
A group of channels are respectively and independently arranged on two sides of the main optical path axis in the optical path support 101, and an optical component in each group of channels comprises an excitation light source, an excitation lens, a detection optical filter, a detector, a detection lens and an excitation optical filter; one side of the first dichroic mirror 103 or the third dichroic mirror 105 is sequentially provided with an excitation optical filter, an excitation lens and an excitation light source from near to far along the axis of the main light path; one side of the second dichroic mirror 104 or the fourth dichroic mirror 106 is sequentially provided with a detection optical filter, a detection lens and a detector from near to far along the axis of the main light path.
The excitation light source is fixedly arranged on the focal length of the laser lens, the detector is fixedly arranged on the focal length of the detection lens, and the detection lens and the laser lens are fixedly arranged inside the lens sleeve and are respectively and independently set to be a biconvex lens or a plano-convex lens.
The focusing lens 102 is configured as a biconvex lens or a plano-convex lens. The excitation light source is any one of a light emitting diode, a laser, and a halogen tungsten lamp, and is not limited to the above type, and may be other common light sources.
The drawings of the present invention will be described in detail with reference to the embodiments.
Fig. 1 shows the overall structure of the device of the present invention, the box body 3 includes a box cover plate 301 and a box bottom plate 302, which are made of metal material and can effectively shield the external noise interference.
Fig. 2 shows the internal structure diagram of the box body of the device of the present invention, a plurality of mounting holes 119 are provided above and below the light path support 101 for mounting the light path support cover plate 120 and the circuit board 2. The circuit board 2 is positioned below the dual-channel light path structure 1 and comprises a power supply management unit, a light source driving unit, a signal amplification and filtering unit, an analog-to-digital conversion unit, a communication interface unit and a microcontroller core unit; the circuit board 2 is provided with a power supply and a communication interface for supplying power to the device and carrying out data interaction with an external computer or a singlechip.
The circuit board 2 comprises a power supply management unit, a light source driving unit, a signal amplification and filtering unit, an analog-to-digital conversion unit, a communication interface unit and a microcontroller core unit; the power management unit is used for providing power supply power, the input end of the power management unit is communicated with an external power supply device through a power interface, and the output end of the power management unit is electrically connected with the power utilization unit in the circuit board 2; the light source driving units are provided with two paths for driving the excitation light source and are electrically connected with the excitation light source bottom plate 115; the signal amplifying and filtering unit is positioned on the detector bottom plate 116, is electrically connected with the analog-to-digital conversion unit, and is used for converting the fluorescent signal into a digital electric signal which can be processed by the microcontroller; the communication interface unit can adopt RS232, RS485, I2C or SPI communication protocols; the microcontroller can adopt various mainstream singlechips.
Fig. 3 is the utility model discloses the binary channels light path structure and the optical component position map of device, 1 fixed mounting of binary channels optical structure is provided with light path support 101 light path support apron 120, optical component, focusing lens 102 in the bottom of 3 cavity structures of box body, and wherein a light path support 101 tip passes through threaded connection focusing lens 102, can follow the rotatory fixed mounting of screw thread on light path support 101, and the other end is installed in box body 3 completely. The optical circuit mount cover plate 120 is mounted on top of the optical circuit mount 101 through a plurality of sets of mounting holes 119, and the optical components are fixedly mounted in the optical circuit mount 101.
The optical component comprises a first dichroic mirror 103, a second dichroic mirror 104, a third dichroic mirror 105 and a fourth dichroic mirror 106, and a main light path axis which is the optical component along the central direction of the focusing lens 102 is set, wherein the first dichroic mirror 103, the second dichroic mirror 104, the third dichroic mirror 105 and the fourth dichroic mirror 106 are sequentially arranged in the light path support 101 from near to far away from the focusing lens 102; the first dichroic mirror 103 and the second dichroic mirror 104 are arranged in parallel and are respectively and fixedly installed at an included angle of 45 degrees with the axis of the main light path; the third dichroic mirror 105 and the fourth dichroic mirror 106 are arranged in parallel, and are respectively and fixedly installed at an included angle of 135 degrees with the axis of the main light path.
A group of channels are respectively and independently arranged on two sides of the main optical path axis in the optical path support 101, and an optical component in each group of channels comprises an excitation light source, an excitation lens, a detection optical filter, a detector, a detection lens and an excitation optical filter; one side of the first dichroic mirror 103 or the third dichroic mirror 105 is sequentially provided with an excitation optical filter, an excitation lens and an excitation light source from near to far along the axis of the main light path; one side of the second dichroic mirror 104 or the fourth dichroic mirror 106 is sequentially provided with a detection optical filter, a detection lens and a detector from near to far along the axis of the main light path.
The excitation light source is fixedly arranged on the focal length of the laser lens, the detector is fixedly arranged on the focal length of the detection lens, the detection lens and the laser lens are fixedly arranged inside the lens sleeve, and the lenses can be independently selected to have the function of converging and diverging light and are arranged to be double convex lenses or plano-convex lenses, wherein the centers of the lenses coincide with the center of a light path.
The focusing lens 102 is configured as a biconvex lens or a plano-convex lens, and the excitation light source is configured as any one of a light emitting diode, a laser, and a halogen tungsten lamp, which is not limited to the above type, and may be other common light sources. The detector is selected from a photodiode, a photomultiplier, and other devices having a photoelectric conversion function, but is not limited thereto.
As a specific embodiment of the present invention, it may be configured that the optical module includes: a first excitation filter 107, a second excitation filter 108, a first detection filter 109, a second detection filter 110, a first excitation lens 111, a second excitation lens 112, a first detection lens 113, a second detection lens 114, a first excitation light source 115, a second excitation light source 116, a first detector 117, and a second detector 118; first dichroic mirror 103, second dichroic mirror 104, third dichroic mirror 105, fourth dichroic mirror 106 are arranged on the main light path axis, first dichroic mirror 103 is parallel to second dichroic mirror 104 and is placed at an included angle of 45 degrees with the light path, and third dichroic mirror 105 is parallel to fourth dichroic mirror 106 and is placed at an included angle of 135 degrees with the light path.
The first excitation lens 111, the second excitation lens 112, the first detection lens 113, and the second detection lens 114 are each a biconvex lens or a plano-convex lens having a function of converging diverging light, and are installed in a customized lens housing with their centers coinciding with the optical path center.
The first excitation light source 115 and the second excitation light source 116 are soldered on a customized circuit board, and pads are arranged on the edge of the circuit board and correspondingly connected with the pads on the surface of the circuit board 201. The first detector 117 and the second detector 118 are soldered to a custom-made circuit board, and pads are provided on the edge of the board to correspond to the pads on the surface of the circuit board. The first excitation light source 115 is located at the focal length of the first excitation lens 111; the second excitation light source 116 is located at the focal length of the second excitation lens 112; the first detector 117 is located at the focal length of the first detection lens 113; second detector 118 is located at the focal length of second detection lens 114.
Fig. 4 shows that the utility model discloses the binary channels light path principle schematic diagram of device, first dichroic mirror, second dichroic mirror, third dichroic mirror, fourth dichroic mirror are arranged on the main light path axle, and first dichroic mirror is parallel with second dichroic mirror and is 45 degrees contained angles with the light path and places, and third dichroic mirror is parallel with fourth dichroic mirror and is 135 degrees contained angles with the light path and places. The first excitation light source is positioned on the focal length of the first excitation light lens; the second excitation light source is positioned on the focal length of the second excitation light lens; the first detector is positioned on the focal length of the first detection lens; the second detector is located at the focal length of the second detection lens.
The utility model discloses in still provide the application of above-mentioned integrated fluorescence detection device in two kinds of wavelength excitation detection timesharing multiplex or single pass arouse double-circuit and detect, explain through concrete embodiment below.
Example 1: fluorescein sodium concentration detection experiment
This embodiment will the utility model discloses the device is as independent device exclusive use, will the utility model discloses the communication interface of device links to each other with the computer, and the switch on can carry out fluorescein sodium concentration detection experiment under the control of the computer desktop end software of customization, and concrete operating procedure is as follows:
1. preparing a fluorescein sodium solution according to a certain concentration gradient, and subpackaging in a high-permeability centrifuge tube for later use.
2. Sequentially placing the high-transmittance centrifugal tube filled with the fluorescein sodium solution with the known concentration gradient at the focus of a lens in front of a focusing lens, starting a first excitation light source, and simultaneously starting a first detector to perform first-channel fluorescence detection; the microcontroller of the device analyzes and processes the detected fluorescence data and then sends the fluorescence data to desktop software of a computer; and recording the concentration of the fluorescein sodium and the corresponding fluorescence signal intensity by a user, and drawing a concentration-fluorescence signal intensity standard curve.
3. Placing a high-transmittance centrifugal tube filled with a fluorescein sodium solution with unknown concentration at the focus of a lens in front of a focusing lens, starting a first excitation light source, and simultaneously starting a first detector to perform first channel fluorescence detection; the microcontroller of the device analyzes and processes the detected fluorescence data, and then sends the fluorescence data to desktop software of a computer, and a user records the fluorescence signal intensity of the computer; and (3) comparing a concentration-fluorescence intensity standard curve to obtain the concentration of the fluorescein sodium solution to be detected.
Example 2: multiple real-time fluorescence PCR experiment of clostridium difficile
This embodiment will the utility model discloses the device is as functional module, and integrated in the temperature control platform of self-control, found real-time fluorescence PCR platform, will the utility model discloses the power of device links to each other with real-time fluorescence PCR platform main control circuit board with communication interface, can carry out fluorescence detection operation according to the experimental procedure under main control circuit board's control. The specific operation steps are as follows:
1. preparing a real-time fluorescent PCR reagent system: master Mix 12.5. mu.L, Clostridium difficile upstream and downstream primers 1. mu.L each, fluorescent probe 0.5. mu.L, DNA template 2. mu.L, deionized water to make up the system to 20. mu.L. Preparing 3 tubes of PCR reagent according to the system, wherein the No. 1 tube is used as negative control, and the DNA template is replaced by equivalent deionized water; tube 2 is a positive control, and the DNA template is a DNA template with known concentration; the No. 3 tube is a sample tube to be detected, and the DNA template is a DNA sample obtained by upstream nucleic acid extraction.
2. Put into platform linear arrangement's metal heat sink with above-mentioned 3 PCR, the metal heat sink orientation the utility model discloses the side trompil of device one side, the trompil is in the utility model discloses the focus department of the focusing lens of device, and be in same height.
3. The experiment of beginning real-time fluorescence PCR, before the extension stage of each PCR thermal cycle ended, step motor drove the utility model discloses the device carries out the scanning of a round trip to 3 PCR pipes, makes a round trip in one, and first excitation light source is opened to the first half section, opens simultaneously first detector and carries out the fluorescence detection of first passageway, and the first passageway is closed to the latter half section, opens the second excitation light source, opens simultaneously the second detector and carries out the fluorescence detection of second passageway. After scanning is finished, a microcontroller of the device analyzes and processes the fluorescence data detected in the circulation, and then the fluorescence data is sent to a platform main control circuit board, and the main control circuit board sends the fluorescence data to computer control software for real-time fluorescence curve drawing.
4. And (3) repeating the step (3) until the whole real-time fluorescence PCR process is finished, and acquiring the related information of the sample to be detected according to the drawn real-time fluorescence PCR curve.
The embodiment can show that the device of the utility model presets a plurality of working modes through the double-channel design, can realize the time-sharing multiplexing of two-wavelength excitation detection, and can also excite the double-channel detection by a single channel, thereby being suitable for a plurality of application scenes; in addition, the reflector is additionally arranged behind the focusing lens, so that the direction of the focusing lens can be conveniently changed to be upward or downward, and the top excitation detection or the bottom excitation detection can be conveniently applied.
The above-mentioned embodiments only represent some embodiments of the present invention, and the description thereof is specific and detailed, but not to be construed as limiting the scope of the present invention. It should be noted that, for those skilled in the art, without departing from the spirit of the present invention, several variations and modifications can be made, which are within the scope of the present invention. Therefore, the protection scope of the present invention should be subject to the appended claims.
Claims (8)
1. An integrated fluorescence detection device, characterized in that: the optical fiber connector comprises a double-channel optical path structure (1) and a box body (3), wherein the box body (3) is provided with a box body cover plate (301) and a box body bottom plate (302), the box body cover plate (301) is of a cavity structure, the movable cover is arranged at the upper end of the box body bottom plate (302), and one side surface of the movable cover is provided with an opening; binary channels light path structure (1) fixed mounting is provided with light path support (101), light path support apron (120), optical component, focusing lens (102) in box body (3) cavity structure's bottom, wherein light path support (101) a tip is through threaded connection focusing lens (102), and the other end is installed completely in box body (3), light path support apron (120) are installed at light path support (101) top through multiunit mounting hole (119), optical component fixed mounting is in light path support (101).
2. The integrated fluorescence detection device of claim 1, wherein: still include circuit board (2), set up in binary channels light path structure (1) bottom, through fastener detachably with binary channels light path structure (1) and circuit board (2) fixed mounting in the bottom of box body (3) cavity structure.
3. The integrated fluorescence detection device of claim 2, wherein: the circuit board (2) comprises a power supply management unit, a light source driving unit, a signal amplification and filtering unit, an analog-to-digital conversion unit, a communication interface unit and a microcontroller core unit; the power management unit is used for providing power supply power, the input end of the power management unit is communicated with an external power supply device through a power interface, and the output end of the power management unit is electrically connected with the internal power utilization unit of the circuit board (2); the light source driving units are divided into two paths, are used for driving the excitation light source and are electrically connected with the excitation light source bottom plate (115); the signal amplification and filtering unit is positioned on the detector bottom plate (116), is electrically connected with the analog-to-digital conversion unit and is used for converting the fluorescent signal into a digital electric signal which can be processed by the microcontroller; the communication interface unit adopts RS232, RS485, I2C or SPI communication protocol; the microcontroller adopts a single chip microcomputer.
4. The integrated fluorescence detection device of claim 1, wherein: the optical component comprises a first dichroic mirror (103), a second dichroic mirror (104), a third dichroic mirror (105) and a fourth dichroic mirror (106), and a main light path axis of the optical component is set along the central direction of the focusing lens (102), wherein the first dichroic mirror (103), the second dichroic mirror (104), the third dichroic mirror (105) and the fourth dichroic mirror (106) are sequentially arranged in the light path support (101) from near to far away from the focusing lens (102) at a certain distance; the first dichroic mirror (103) and the second dichroic mirror (104) are arranged in parallel and are respectively and fixedly installed at an included angle of 45 degrees with the axis of the main light path; and the third dichroic mirror (105) and the fourth dichroic mirror (106) are arranged in parallel and are respectively and fixedly installed at an included angle of 135 degrees with the axis of the main light path.
5. The integrated fluorescence detection device of claim 4, wherein: a group of channels are respectively and independently arranged on two sides of the main optical path axis in the optical path support (101), and an optical component in each group of channels comprises an excitation light source, an excitation lens, a detection optical filter, a detector, a detection lens and an excitation optical filter; one side of the first dichroic mirror (103) or the third dichroic mirror (105) is sequentially provided with an excitation optical filter, an excitation lens and an excitation light source from near to far along the axis of the main light path; one side of the second dichroic mirror (104) or the fourth dichroic mirror (106) is sequentially provided with a detection optical filter, a detection lens and a detector from near to far along the axis of the main light path.
6. The integrated fluorescence detection device of claim 5, wherein: the excitation light source is fixedly arranged on the focal length of the laser lens, the detector is fixedly arranged on the focal length of the detection lens, and the detection lens and the laser lens are fixedly arranged inside the lens sleeve and are respectively and independently set to be a biconvex lens or a plano-convex lens.
7. The integrated fluorescence detection device of claim 1, wherein: the focusing lens (102) is arranged to be a biconvex lens or a plano-convex lens.
8. The integrated fluorescence detection device of claim 5, wherein: the excitation light source is any one of a light-emitting diode, a laser and a halogen tungsten lamp, and the detector is a photodiode or a photomultiplier.
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| CN202021532071.3U CN212301289U (en) | 2020-07-29 | 2020-07-29 | Integrated fluorescence detection device |
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| CN202021532071.3U CN212301289U (en) | 2020-07-29 | 2020-07-29 | Integrated fluorescence detection device |
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