CN112245449A

CN112245449A - Preparation method and application of zedoary turmeric polysaccharide

Info

Publication number: CN112245449A
Application number: CN202011533064.XA
Authority: CN
Inventors: 达卓玛; 旦增洛布; 平措南加; 尼玛拉宗; 格桑坚赞
Original assignee: Jiangxi Yeli Medical Instrument Co ltd
Current assignee: TIBET GANLU TIBETAN MEDICINE Co.,Ltd.
Priority date: 2020-12-23
Filing date: 2020-12-23
Publication date: 2021-01-22
Anticipated expiration: 2040-12-23
Also published as: CN112245449B

Abstract

The invention relates to the technical field of plant polysaccharide preparation, in particular to a preparation method and application of curcuma zedoary polysaccharide. The zedoary turmeric herb is extruded and puffed by a double screw extruder for pretreatment to prepare dry paste, and then the zedoary turmeric herb polysaccharide is prepared, so that the yield and the activity of the zedoary turmeric polysaccharide can be obviously improved, the macromolecular polysaccharide is hydrolyzed by using organic acid to obtain high-activity micromolecular polysaccharide, the zedoary turmeric herb dry paste and the polysaccharide prepared by the application have excellent analgesic effect, and the zedoary turmeric polysaccharide obtained by compounding the technical scheme can delay aging.

Description

Preparation method and application of zedoary turmeric polysaccharide

Technical Field

The invention relates to the technical field of plant polysaccharide preparation, in particular to a preparation method and application of curdla polysaccharide.

Background

E Zhu Xia (rhizoma Curcumae)OxytropischiliophyllaRoyle ex Benth) The acanthopanax senticosus, the crazyweed, the quda mao, the deji bara, the cuminum cyminum and the like are acanthopanax of sphenoidaceae of the order of glycines, grow on beaches, sand lands, valleys, hills, shrubs and meadows with the altitude of 2700-4300 m, and are distributed in Qinghai, Tibet, Gansu, Sichuan and other places. The Tibetan medicine is called the king of the herbal medicine, is mainly used for healing sore, facilitating defecation, removing toxicity, clearing swelling and stopping bleeding, can treat various diseases such as anthracnose, leprosy, influenza, tonsillitis and the like, and is the first choice of the Tibetan medicine for treating tuberculosis. The Zhu Xia is recorded in the classical famous works of Tibetan medicine. For example, it is recorded in Tibetan Yao Zhi to treat high fever, laryngitis, impetigo, hematochezia, red and white dysentery, bleeding due to wound, and it can be used for external application to relieve swelling and pain, remove putrefaction and promote tissue regeneration, and treat knife wound. Also, as recorded in "Zhuomaben", it can treat the king of energy efficiency medicine, some are called Daxiawa, some are called Daji acaba, the correct name is Qudao, the high mountain yin is growing equally, the essential taste and performance is, the leaves are red and blue, the flower is red and thick, the grass ear triangle has good efficacy, the fruit is greasy when cooked. "there is also" statement "that" Da Xia Wa grows on grassland, the leaves are like the leaves of the mountain beans, spread over the whole land, the buds are flat like the mountain beans, the leaves are cut off and the tree roots are like the roots of mugwort. "Nu Ben" records that "black color reaches the middle-long part of the summer valley, leaves flower like small bean, long and short means five fingers long, smells smelly and has large viscosity. 'Zhongshe' records that the Wangda Xiawa in the herbs, the dry mountain and the grassland grow, the leaves are thick and thin and are all around, the flowers are purple like the mountain beans, the odor is big, the roots are fragrant, the hands are oily and oozed out, and the herbs for stopping bleeding and inflammation are dry yellow water, sores and abscesses. "Jingzhu materia Medica" is born in arid flat beach, hillside or mountain and has thick and thin leavesLike crushed pine rock; the flower is purple, is similar to astragalus, has large smell, is provided with mucus when being pinched, and is divided into black and white, the shapes of the two are basically the same, the body is large, the mild character is white, the body is small, and the smell is small, the body is black. "

The zedoary turmeric has bitter and sweet taste in summer, bitter taste after digestion, cold nature and toxicity. E Da Xia clears heat and toxic materials, promotes tissue regeneration and heals sore, astringes vessels to stop bleeding. It is mainly used for treating pestilence, sore throat, constipation, and sore and furuncle by external application. At present, the medicinal mode of the zedoary turmeric comprises entering pills and powder, wherein the zedoary turmeric is mainly prepared by a decoction method, and then the zedoary turmeric is mixed with other medicinal materials for preparing the medicine. For example, the Xiandaxia concrete is used to prepare the Qingchangxiang of zang medicine.

However, the zedoary turmeric extractum extracted in the prior art is troublesome to store and transport, is easy to breed microorganisms, is easy to deteriorate, is not favorable for storage and transport and control of dosage, and the stability and effectiveness of the medicine need to be improved. Therefore, how to improve the stability and effectiveness of the zedoary turmeric medicine and make the product convenient for storage and transportation is a technical problem to be solved at present.

In addition, most of the Tibetan medicine materials are prepared by processing methods, however, the prior art shows that the zedoary turmeric after processing does not exert obvious analgesic effect and shows that the analgesic effect disappears after processing, so that the method for processing the Tibetan medicine zedoary turmeric by using modern means is necessary to achieve the purpose of not damaging the efficacy of the medicine.

The above background disclosure is intended to aid in understanding the inventive concepts and aspects of the invention, and is not necessarily prior art to the present application, and should not be used to assess novelty or inventiveness of the present application without providing explicit evidence that the above disclosure is made at the filing date of the present application.

Disclosure of Invention

Technical problem to be solved

The invention aims to solve at least one technical problem in the background art and provides a preparation method of curcuma zedoaria polysaccharide, which is characterized in that curcuma zedoaria medicinal material is extruded and puffed by a double screw extruder for pretreatment to prepare dry paste, and then the curcuma zedoaria polysaccharide is prepared, so that the yield and the activity of the curcuma zedoaria polysaccharide can be obviously improved, and the macromolecular polysaccharide is hydrolyzed by using organic acid to obtain the high-activity micromolecular polysaccharide.

In addition, the invention also provides application of the zedoary turmeric polysaccharide in preparation of analgesic preparations, compared with the traditional method for processing the Tibetan medicine, the zedoary turmeric dry paste and the polysaccharide prepared by the method have excellent analgesic effect, the characteristics and properties of the medicinal materials are not obviously changed, and a new direction is provided for analgesic research of the zedoary turmeric.

In addition, the invention also provides application of the zedoary turmeric polysaccharide in preparing anti-aging preparations, and after the small molecular zedoary turmeric polysaccharide a and the small molecular zedoary turmeric polysaccharide b obtained by the technical scheme are compounded, the organism immunity of an animal model can be obviously enhanced, the organism oxidation resistance is improved, redundant free radicals are eliminated, and aging is delayed.

Furthermore, the invention aims to solve the problems of inconvenient transportation and storage, easy microorganism breeding, difficult accurate control of the drug dosage, low standardization level and the like of the existing zedoary turmeric extract in the background art, which seriously restrict the modernization construction of the Tibetan medicine, and the invention can effectively solve the problems and improve the standardization level of the Tibetan medicine.

(II) technical scheme

In order to solve the above technical problems or to achieve the above technical object, the present invention provides the following technical solutions.

(1) The method for extracting the zedoary turmeric polysaccharide comprises the following steps:

firstly, degreasing zedoary turmeric powder, adding water, decocting and filtering to obtain a filtrate;

step two, stirring, concentrating and filtering the liquid medicine to obtain extract;

step three, decocting and concentrating the extract to obtain a dry paste semi-finished product;

step four, drying the semi-finished dry paste to obtain the zedoary turmeric root tuber dry paste;

adding 95% ethanol into the zedoary turmeric dry paste until the ethanol content is not lower than 90%, refrigerating overnight, separating to obtain a filtrate I and a filter residue I, adding water into the filter residue I for water extraction, and filtering at room temperature to obtain a filtrate II and a filter residue II; the filtrate I can be used for extracting zedoary turmeric alkaloid and other substances;

sixthly, concentrating the filtrate II to 20-40% of volume, intercepting the macromolecular polysaccharide of the filtrate II by using a 30KDa ultrafiltration membrane, adding absolute ethyl alcohol into the filtrate until the content of the ethyl alcohol is not lower than 90%, and carrying out alcohol precipitation separation to obtain micromolecular curcuma zedoary polysaccharide a;

step seven, combining the filter residue II and the macromolecular polysaccharide obtained in the step six, adding acid until the pH value is not higher than 4, hydrolyzing, and filtering at room temperature to obtain a filtrate III and a filter residue III; adding water into the filter residue III for water extraction, filtering at room temperature to obtain a filtrate IV and a filter residue IV, combining the filtrate III and the filtrate IV, concentrating to 15-30% of volume, adding absolute ethanol into the filtrate intercepted by a 30KDa ultrafiltration membrane until the alcohol content is not lower than 90%, and carrying out alcohol precipitation separation to obtain the small molecular zedoary and rhizoma corydalis Decumbentis polysaccharide b.

In some embodiments of the invention, before the first step of decocting with water, the method further comprises performing extrusion pretreatment on the kaempferia pandurata, specifically, drying the kaempferia pandurata to a water content of 15-18%, performing extrusion pretreatment by using a twin-screw extruder, and crushing to pass through a 100-mesh sieve.

In other embodiments of the invention, the feeding speed of the twin-screw extruder during the extrusion and expansion pretreatment is 5-10 kg/h, the screw rotating speed is 300-600 r/min, and the extrusion temperature is 110-120 ℃.

The inventor finds that before the curvain polysaccharide is extracted, the extrusion and expansion pretreatment of the twin-screw extruder can obviously improve the yield and the activity of the small molecule curvain polysaccharide b, and the extrusion and expansion of the twin-screw extruder can help the loosening and the pre-dissociation of the tissue structure of the curvain, so that the separation of macromolecular polysaccharide is facilitated in the subsequent processes of decoction, alcohol extraction, water extraction and the like, the yield of the small molecule curvain polysaccharide b obtained after acid hydrolysis is higher, and the improvement of the yield and the activity of the small molecule curvain polysaccharide b is helpful for the exertion of the drug effect of the curvain polysaccharide and the high-level application.

In some embodiments of the invention, step one specifically comprises,

degreasing the zedoary turmeric powder, adding deionized water with the weight being 9-11 times that of the medicinal materials for first decoction, and filtering to obtain medicine residue I and medicine liquid I;

adding the residue I into water 9-11 times of the weight of the medicinal materials for second decoction, and filtering to obtain a residue II and a liquid medicine II;

mixing the liquid medicine I and the liquid medicine II together to obtain the filtered liquid medicine.

In some embodiments of the present invention, in the second step, the filtering liquid medicine is stirred and concentrated until the density of the filtering liquid medicine reaches 1.01g/mL or more, and then the concentration is completed;

in some embodiments of the present invention, in step three, the extract is decocted and concentrated at a decocting temperature of 170-190 ℃ and stirred, and the decocting temperature is adjusted according to density variation of the extract, specifically:

when the density of the extract is 1.01-1.05 g/mL, controlling the decoction temperature to be 180 ℃;

when the density of the extract is 1.06-1.12 g/mL, controlling the decoction temperature to be 160 ℃;

when the density of the extract is 1.13-1.22 g/mL, controlling the decoction temperature to be 130 ℃;

when the density of the extract reaches 1.23/mL or more, controlling the decoction temperature to be 120 ℃;

and when the extract does not emit steam, decocting and concentrating to finish.

In some embodiments of the invention, in step four,

the standing time of the semi-finished dry paste is 6h,

the drying mode is electric heating forced air drying or vacuum drying or natural drying in the shade;

the drying temperature of the electric heating blast drying is 60-80 ℃, and the drying time is 4-8 d;

the vacuum drying temperature is 60-80 ℃, the drying pressure is 0.05MPa, and the drying time is 4-8 d;

and the drying time of the natural drying in the shade is 5-25 d.

In some embodiments of the invention, in the fifth step, the temperature for refrigerating overnight is 1-4 ℃, and the time is not less than 12 h.

According to some embodiments of the invention, in the fifth step, the ratio of the water to the feed liquid is 1: 20-50, the water extraction temperature is 55-60 ℃, and the extraction is performed for 3 times (1-2 h).

In some embodiments of the present invention, in the sixth step, the filtrate ii may be concentrated by low temperature rotary steaming, decocting and concentrating.

According to some embodiments of the invention, in the sixth step, during alcohol precipitation separation, the mixture is stirred for 10min at a speed of 300-900 r/min, and then is kept stand for 1-2 h at room temperature, and the precipitate is taken out.

In some embodiments of the invention, in the seventh step, the acid is citric acid or malic acid, the hydrolysis temperature is 55-60 ℃, and the hydrolysis time is 45-60 min; further experiments by the inventor show that, by adding organic acid, especially citric acid or malic acid, to hydrolyze the macromolecular polysaccharide, it can not only obtain more excellent hydrolysis effect and increase the yield of the small molecular zedoary polysaccharide, but also avoid the corrosion of the acid solution with lower pH to the container, and is beneficial to the subsequent cleaning, the treatment of the acid solution, etc., and the process is simplified and is also beneficial to the environment, compared with strong acid (such as hydrochloric acid or sulfuric acid) under the condition of lower pH (such as 2 or even below 1.5).

According to some embodiments of the invention, in the seventh step, the ratio of the water to the feed liquid is 1: 15-30, the temperature of the water extraction is 60-65 ℃, and the water extraction is carried out for 2-3 hours.

In some embodiments of the present invention, in step seven, the filtrate iii and the filtrate iv may be combined and concentrated by low temperature rotary evaporation, decoction and concentration.

According to some embodiments of the invention, in the seventh step, during alcohol precipitation separation, the mixture is stirred for 10min at a speed of 300-900 r/min, and then is kept stand for 1-2 h at room temperature, and the precipitate is taken out.

According to the technical scheme, the zedoary turmeric root tuber medicinal material is added with water for decoction and filtration to obtain filtered liquid medicine, then the liquid medicine is stirred and concentrated to obtain extract, and the decoction temperature is controlled according to the density change of the extract during decoction and concentration of the extract until no steam emerges from the extract, so that a dry extract semi-finished product is obtained; standing the semi-finished dry paste, taking out, and drying to obtain the rhizoma zedoariae and rhizoma corydalis-summer dry paste. The prepared zedoary turmeric dry paste can replace a zedoary turmeric extract for use, is convenient to store and transport, can accurately control the dosage, has a simple preparation method, is easy to popularize, and can improve the stability and effectiveness of a medicine.

Further, on the basis of the zedoary turmeric dry paste, the zedoary turmeric dry paste is subjected to alcohol precipitation, water extraction, ultrafiltration and alcohol precipitation to prepare the small molecular zedoary turmeric polysaccharide a, partial filtrate and the macromolecular polysaccharide are merged and hydrolyzed by strong acid, and after water extraction, ultrafiltration and alcohol precipitation, separating to obtain the small molecular zedoary turmeric polysaccharide b hydrolyzed from the macromolecular polysaccharide, performing ultrafiltration with a 30KDa large-aperture ultrafiltration membrane, without performing multiple times of more refined separation such as ultrafiltration and nanofiltration, but the yield of the small molecule zedoary turmeric polysaccharide is obviously increased through the hot acid hydrolysis, not only the protein is effectively removed, but also has excellent decolorization effect, and compared with the traditional method for extracting the curdla polysaccharide by alcohol precipitation, the method not only omits the steps of protein removal and decolorization, the small molecule zedoary polysaccharide a and the small molecule zedoary polysaccharide b also have excellent properties which are not possessed by the zedoary polysaccharide obtained by the traditional alcohol precipitation method.

(2) And the application of the zedoary turmeric dry paste obtained by the extraction method of the zedoary turmeric polysaccharide in preparing analgesic preparations.

(3) And the application of the curdla polysaccharide obtained by the curdla polysaccharide extraction method in preparing analgesic preparations.

It is known that, after the Tibetan medicine is processed by the milk preparation method in the general rules of processing, the analgesic effect of the zedoary turmeric has relatively obvious attenuation, even basically no obvious analgesic effect; however, the kaempferia galamga dry paste and the small molecular kaempferia galamga polysaccharide obtained according to the technical scheme of the application have excellent analgesic effect, the characteristics and properties of the medicinal materials are not obviously changed, and a new direction is provided for the research of kaempferia galamga analgesic.

(4) And the application of the small molecular zedoary turmeric polysaccharide a and the small molecular zedoary turmeric polysaccharide b obtained by the extraction method of the zedoary turmeric polysaccharide in preparing the anti-aging preparation.

In some embodiments of the present invention, the anti-aging preparation comprises the small molecular zedoary polysaccharide a and the small molecular zedoary polysaccharide b in a weight ratio of 3 to 5:1, preferably 3.5 to 4.5:1, more preferably 3.8 to 4.5:1, and most preferably 4: 1.

The inventor finds that unexpected and excellent anti-aging effect can be obtained by compounding the small molecule zedoary polysaccharide a and the small molecule zedoary polysaccharide b obtained by the technical scheme, and animal models show that the compound preparation can obviously improve the thymus index and the spleen index of an aging molding mouse, the blood SOD (superoxide dismutase) activity, the GSH-Px (glutathione peroxide oxidase) activity, the CAT (catalase) activity of brain tissues and the like of the mouse are obviously improved, and the blood MDA (malondialdehyde) content is lower, so that the preparation is beneficial to enhancing the immune function of the body, improving the anti-oxidation capability of the body, eliminating redundant free radicals and delaying aging.

The above-described preferred conditions may be combined with each other to obtain a specific embodiment, in accordance with common knowledge in the art.

The raw materials or reagents involved in the invention are all common commercial products, and the operations involved are all routine operations in the field unless otherwise specified.

(III) advantageous effects

The technical scheme of the invention has the following advantages:

1) the preparation method comprises the steps of adding water into the zedoary turmeric herb medicinal material, decocting and filtering to obtain a filtrate, stirring and concentrating the filtrate to obtain an extract, controlling the decocting temperature according to the density change of the extract during the decoction and concentration of the extract until no steam emerges from the extract to obtain a semi-finished dry extract product, and drying the semi-finished dry extract product to obtain the zedoary turmeric herb dry extract product; the zedoary turmeric dry paste obtained by the invention has the advantages of convenient storage, convenient transportation, easy popularization, accurate medicine injection, good stability, high standardization, easy control of the safety and effectiveness of medicines and the like, and can be used for replacing extractum;

2) before the curare polysaccharide is extracted, the yield and the activity of the small molecule curare polysaccharide b can be obviously improved by extrusion and puffing pretreatment of a double-screw extruder, and the improvement of the yield and the activity is beneficial to the exertion of the drug effect of the curare polysaccharide and high-level application;

3) the macromolecular polysaccharide is hydrolyzed by adding organic acid, particularly citric acid or malic acid, and compared with strong acid (such as hydrochloric acid or sulfuric acid), under the condition of lower pH (such as below 2 and even 1.5), the macromolecular polysaccharide has more excellent hydrolysis effect, the yield of the final product, namely the micromolecular polysaccharide zedoaria can be improved, the corrosion of acid liquor with lower pH on a container can be avoided, the subsequent cleaning, the treatment of the acid liquor and the like can be facilitated, the process is simplified, and the environment is benefited;

4) the method only uses a 30KDa large-aperture ultrafiltration membrane for ultrafiltration, does not perform more refined separation such as multiple times of ultrafiltration, nanofiltration and the like, but remarkably increases the yield of the small molecule zedoary turmeric polysaccharide through hot acid hydrolysis, not only effectively removes proteins, but also has excellent decolorization, and not only omits the steps of protein removal and decolorization compared with the traditional method for extracting the zedoary turmeric polysaccharide by alcohol precipitation, but also has excellent properties which are not possessed by the traditional method for obtaining the zedoary turmeric polysaccharide by alcohol precipitation;

5) the curcuma zedoaria dry paste and the small molecular curcuma zedoaria polysaccharide obtained according to the technical scheme of the application have excellent analgesic effect, the characteristics and properties of the medicinal materials are not obviously changed, and a new direction is provided for the analgesic research of the curcuma zedoaria;

6) the animal model shows that the compound preparation can obviously improve the thymus index and spleen index of an aging molding mouse, the blood SOD activity, the liver tissue GSH-Px activity, the brain tissue CAT activity and the like of the mouse are obviously improved, and the blood MDA content is lower, so that the compound zedoary polysaccharide a and the compound zedoary polysaccharide b are beneficial to enhancing the body immunity, improving the body oxidation resistance, eliminating redundant free radicals and delaying aging.

The invention adopts the technical scheme for achieving the purpose, makes up the defects of the prior art, and has reasonable design and convenient operation.

Drawings

The foregoing and/or other objects, features, advantages and embodiments of the invention will be more readily understood from the following description taken in conjunction with the accompanying drawings in which:

FIG. 1 is a flow chart showing the process for preparing an extract proposed in example 1 of the present invention;

FIG. 2 is a flow chart showing a process for preparing a dry paste proposed in example 1 of the present invention;

FIG. 3 is a graph showing the density of the extract according to the embodiment 1 of the present invention as a function of temperature; the abscissa is density (unit: g/mL); the ordinate is temperature (unit:. degree. C.);

FIG. 4 is a graph showing the effect of curdlan on mouse thymus index and spleen index; the horizontal coordinate is the animal group; the ordinate is the index value (unit: mg/g); 4A is thymus index and 4B is spleen index.

Detailed Description

The endpoints of any ranges and any values disclosed in the present application are not limited to the precise range and/or value, and such ranges and/or values can and should be understood to encompass values close to those ranges and/or values. For ranges of values, between the endpoints of each of the ranges and the individual points, and between the individual points may be combined with each other to give one or more new ranges of values, and these ranges of values should be considered as specifically disclosed herein.

The technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application, and it is obvious that the described embodiments are only a part of the embodiments of the present application, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present application.

Because the technical problems that the zedoary turmeric extractum extracted according to the zedoary turmeric is not beneficial to storage and transportation and control of the dosage exist in the prior art, the invention provides the zedoary turmeric dry paste, which is prepared by the following steps:

step one, adding water into zedoary turmeric herb for decoction and filtration to obtain a filtered liquid medicine;

in a specific implementation scene, adding water 9-11 times of the weight of the zedoary turmeric into the zedoary turmeric root tuber medicinal material for first decoction, wherein the decoction temperature is 190 ℃, the decoction time is 80-100 min, stirring once every 20min at 60-150 r/min for 2min once, and filtering with a 100-mesh sieve to obtain medicine residue I and medicine liquid I; adding the residue I into water 9-11 times of the weight of the medicinal materials for second decoction, wherein the decoction temperature is 190 ℃, the decoction time is 50-70 min, stirring once every 20min at 60-150 r/min, and filtering for 2min once by using a 100-mesh sieve to obtain residue II and liquid medicine II; mixing the liquid medicine I and the liquid medicine II together to obtain the filtered liquid medicine;

step two, stirring and concentrating the liquid medicine to obtain an extract;

in a specific implementation scene, stirring and concentrating the filtered liquid medicine, wherein the concentration temperature is 190 ℃, stirring once every 20min at 60-150 r/min for 2min until the density of the filtered liquid medicine reaches more than 1.01g/mL, and then concentrating;

step three, decocting and concentrating the extract, and controlling the decocting temperature according to the density change of the extract to obtain a dry extract semi-finished product;

in a specific implementation scene, the extract is decocted and concentrated at the decocting temperature of 170-190 ℃ and stirred at 60-150 r/min, and the density of the extract is detected; adjusting the decoction temperature according to the density change of the extract; the method specifically comprises the following steps:

the density of the extract reaches 1.01g/mL, and the decoction temperature is controlled to be 180 ℃;

the density of the extract reaches 1.10g/mL, and the decoction temperature is controlled to be 160 ℃;

the density of the extract reaches 1.15g/mL, and the decoction temperature is controlled to be 130 ℃;

the density of the extract reaches 1.23g/mL, and the decoction temperature is controlled to be 120 ℃;

when the extract does not emit steam, the decoction and the concentration are finished;

step four, standing the semi-finished dry paste, taking out, and drying to obtain the zedoary turmeric dry paste;

in a specific implementation scene, the standing time of the dry paste semi-finished product is 6 hours, and the drying mode is electrothermal blowing drying, vacuum drying or natural shade drying; the preferable drying mode is electrothermal blowing drying, the drying temperature of the electrothermal blowing drying is 60-80 ℃, and the drying time is 4-8 d; and the vacuum drying temperature is 60-80 ℃, the drying pressure is 0.05MPa, and the drying time is 4-8 d.

Through the application of the technical scheme, the zedoary turmeric root tuber medicinal material is added with water for decoction and filtration to obtain a filtered liquid medicine; stirring and concentrating the liquid medicine to obtain an extract; decocting and concentrating the extract, and controlling the decocting temperature according to the density change of the extract to obtain a dry extract semi-finished product; and standing the semi-finished dry paste, taking out, and drying to obtain the zedoary turmeric dry paste. The prepared zedoary turmeric dry paste can replace a zedoary turmeric extract for use, is convenient to store and transport, can accurately control the dosage, has a simple preparation method, is easy to popularize, and can improve the stability and effectiveness of a medicine.

The following describes in detail specific embodiments of the present invention. It should be understood that the detailed description and specific examples, while indicating the present invention, are intended for purposes of illustration and explanation only and are not intended to limit the scope of the invention.

The present invention is described in detail below.

Example 1: a zedoary turmeric root tuber and rhizoma zedoariae dry paste:

this example provides a dry paste of zedoary turmeric, which is prepared by the following steps, as shown in fig. 1 and fig. 2:

step one, pretreating 2.5kg of zedoary turmeric herb, adding 25L of water into a 500L jacketed kettle, and carrying out first decoction at 190 ℃ for 80-100 min while stirring every 20 min. After decocting, filtering for the first time (100 mesh sieve) to obtain medicine residue I and medicine liquid I.

Step two, adding 25L of water into the medicine residue I, and carrying out second decoction, wherein the decoction temperature is 190 ℃, the decoction time is 50-70 min, and stirring is carried out once every 20 min; and filtering for the second time (100 mesh) after decoction to obtain medicine residue II and medicine liquid II.

And step three, mixing the liquid medicine I and the liquid medicine II together to obtain filtered liquid medicine, and discarding the medicine residue II.

And step four, adding the filtered liquid medicine into a 300L jacketed kettle for concentration, stirring once every 20min at the concentration temperature of 190 ℃, measuring the density of the filtered liquid medicine, increasing the density of the liquid medicine along with the continuous concentration of the filtered liquid medicine, and finishing the concentration when the density of the filtered liquid medicine reaches more than 1.01g/mL to obtain the extract.

And step five, adding the extract into a 300L jacketed kettle for decoction and concentration, setting the decoction temperature to be 180 ℃, stirring once every 10min, detecting the density of the extract, and regulating the decoction temperature downward according to the density change of the extract.

The control table of the density and temperature of the extract is shown in Table 1, and the graph of the density of the extract with the change of temperature is shown in FIG. 3.

TABLE 1 decoction temperature and extract Density

In particular, the method comprises the following steps of,

when the density of the extract reaches 1.01g/mL, controlling the decoction temperature to be 180 ℃;

when the density of the extract reaches 1.10g/mL, controlling the decoction temperature to be 160 ℃;

when the density of the extract reaches 1.15g/mL, controlling the decoction temperature to be 130 ℃;

when the density of the extract reaches 1.23g/mL, controlling the decoction temperature to be 120 ℃;

the higher the density of the extract is, the faster the stirring frequency is, the stirring frequency can be properly adjusted according to the density, and when the density of the extract reaches 1.15g/mL, the stirring is continued. When the extract has no steam, the decoction and the concentration are finished to obtain a semi-finished dry extract.

It should be noted that the density and temperature control table of the extract is a preferred embodiment of the present application, and can be adjusted according to specific implementation scenarios, and other ways of adjusting the decocting temperature according to the density change of the extract all belong to the protection scope of the present application.

And step six, standing the semi-finished product of the dry paste for 6 hours, taking out, carrying out electric heating forced air drying at the drying temperature of 60 ℃ for 4-8 days, and collecting the paste after drying to obtain the zedoary turmeric dry paste.

The zedoary turmeric ointment provided by the application can be used for replacing a zedoary turmeric extract in the prior art or mixed with other medicines for preparing the medicine for treating the traditional Chinese medicine, such as preparing the traditional Chinese medicine kernel Qingchangjue by using the zedoary turmeric ointment provided by the application for replacing the zedoary turmeric extract. It mainly has the following advantages;

storage advantages: the zedoary turmeric root paste can be stored conveniently to a great extent, the storage time is prolonged, and the utilization rate of the raw medicinal materials is improved. The defects of troublesome storage of extractum, easy breeding of microorganisms, easy deterioration and the like are avoided.

Transportation advantages are as follows: the zedoary turmeric root tuber and rhizoma zedoariae dry paste can be conveniently transported, and the loss in the transportation process is reduced. Meanwhile, the transportation distance and the transportation time can be increased on the premise of ensuring the quality of the medicinal materials.

The medicine is accurate: the zedoary turmeric root tuber and rhizoma zedoariae dry paste can be accurately taken according to a prescription, and various parameters such as the dosage, the medication concentration and the like can be accurately controlled, so that the uniform and stable quality of the medicine is ensured.

The advantages are beneficial to ensuring the stability and effectiveness of the Tibetan medicine, improving the product quality, ensuring the uniform and stable medicine quality, and the preparation method of the zedoary turmeric root paste provided by the application is simple and easy to popularize.

Example 2: zedoary turmeric polysaccharide:

this example provides a curdlan prepared by the following steps:

drying the zedoary turmeric to the water content of 16%, performing extrusion and puffing pretreatment by a double-screw extruder under the conditions of a feeding speed of 10kg/h, a screw rotating speed of 500r/min and an extrusion temperature of 115 ℃, and crushing to pass through a 100-mesh sieve;

step two, pretreating 2kg of the zedoary turmeric herb powder obtained in the step one, adding 20L of water into a 500L jacketed kettle, carrying out first decoction, wherein the decoction temperature is 190 ℃, the decoction time is 100min, stirring for 2min at 120r/min every 20min, and filtering by a 100-mesh sieve to obtain medicine residue I and medicine liquid I; adding the residue I into 20L water, decocting at 190 deg.C for 60min, stirring at 120r/min every 20min for 2min, and filtering with 100 mesh sieve to obtain residue II and medicinal liquid II; mixing the liquid medicine I and the liquid medicine II together to obtain the filtered liquid medicine;

step three, adding the filtered liquid medicine into a 300L jacketed kettle for concentration, wherein the concentration temperature is 190 ℃, stirring for 2min at 120r/min every 20min, measuring the density of the filtered liquid medicine, increasing the density of the liquid medicine along with the continuous concentration of the filtered liquid medicine, and finishing the concentration when the density of the filtered liquid medicine reaches more than 1.01g/mL to obtain an extract;

step four, adding the extract into a 300L jacketed kettle for decoction and concentration, setting the decoction temperature to be 180 ℃, stirring for 2min at 120r/min every 10min, detecting the density of the extract, regulating the decoction temperature according to the density change of the extract, wherein the density and temperature control table of the extract is shown in Table 2; the higher the density of the extract is, the faster the stirring frequency is (the stirring speed is 120r/min when the density is 1.01-1.10 g/mL, the stirring speed is 150r/min when the density is 1.10-1.15 g/mL, and so on), the stirring frequency can be properly adjusted according to the density, and when the density of the extract reaches 1.15g/mL, the stirring is continued. When the extract has no steam, decocting and concentrating to obtain semi-finished dry extract;

TABLE 2 decoction temperature, extract density and agitation frequency

Step five, taking out the semi-finished dry paste after standing for 6 hours, and carrying out vacuum drying at the temperature of 65 ℃, the drying pressure of 0.05MPa and the drying time of 4d to obtain the zedoary turmeric dry paste;

adding 95% ethanol into the zedoary turmeric dry paste until the ethanol content is 90%, refrigerating at 1 ℃ overnight for 12h, separating to obtain a filtrate I and a filter residue I, adding water into the filter residue I according to the material-liquid ratio of 1:20, extracting with water at 55 ℃ for 1h multiplied by 3 times, combining and filtering at room temperature to obtain a filtrate II and a filter residue II; the filtrate I can be used for extracting zedoary turmeric alkaloid and other substances;

seventhly, decocting and concentrating the filtrate II at 110 ℃ to 30% volume, intercepting the macromolecular polysaccharide of the filtrate II by using a 30KDa ultrafiltration membrane, adding absolute ethyl alcohol into the filtrate until the content of the ethanol is 90%, stirring for 10min at 600r/min, standing for 2h at room temperature, and separating and taking precipitate to obtain micromolecular zedoary turmeric polysaccharide a;

step eight, combining the filter residue II and the macromolecular polysaccharide obtained in the step seven, adding malic acid until the pH is =4, heating to 55 ℃, hydrolyzing for 60min, cooling to room temperature, and filtering to obtain a filtrate III and a filter residue III; adding water into filter residue III according to a material-liquid ratio of 1:20, extracting with water at 65 ℃ for 2h, filtering at room temperature to obtain filtrate IV and filter residue IV, mixing filtrate III and filtrate IV, decocting at 110 ℃ and concentrating to 20% volume, adding absolute ethanol into the filtrate after 30KDa ultrafiltration membrane interception until the alcohol content is 90%, stirring at 600r/min for 10min, standing at room temperature for 2h, and separating and collecting precipitate to obtain small molecular weight zedoary polysaccharide b.

Example 3: another zedoary turmeric polysaccharide:

the present embodiment provides another zedoary turmeric polysaccharide, which includes a small molecule zedoary turmeric polysaccharide a and a small molecule zedoary turmeric polysaccharide b, and the preparation method thereof specifically includes:

drying the zedoary turmeric to the water content of 10%, performing extrusion and puffing pretreatment by a double-screw extruder under the conditions of a feeding speed of 10kg/h, a screw rotating speed of 500r/min and an extrusion temperature of 115 ℃, and crushing to pass through a 100-mesh sieve;

steps two to eight are the same as in example 2.

Example 4: another zedoary turmeric polysaccharide:

drying the zedoary turmeric to the water content of 5%, performing extrusion and puffing pretreatment by a double-screw extruder under the conditions of a feeding speed of 10kg/h, a screw rotating speed of 500r/min and an extrusion temperature of 115 ℃, and crushing to pass through a 100-mesh sieve;

steps two to eight are the same as in example 2.

Example 5: another zedoary turmeric polysaccharide:

drying the zedoary turmeric to the water content of 16%, performing extrusion and puffing pretreatment by a double-screw extruder under the conditions of a feeding speed of 10kg/h, a screw rotating speed of 200r/min and an extrusion temperature of 115 ℃, and crushing to pass through a 100-mesh sieve;

steps two to eight are the same as in example 2.

Example 6: another zedoary turmeric polysaccharide:

drying the zedoary turmeric to the water content of 16%, performing extrusion and puffing pretreatment by a double-screw extruder under the conditions of a feeding speed of 10kg/h, a screw rotating speed of 500r/min and an extrusion temperature of 90 ℃, and crushing to pass through a 100-mesh sieve;

steps two to eight are the same as in example 2.

Example 7: another zedoary turmeric polysaccharide:

step one, drying the zedoary turmeric to the water content of 16%, and crushing to pass through a 100-mesh sieve;

steps two to eight are the same as in example 2.

Example 8: another zedoary turmeric polysaccharide:

step one, the same as step one of embodiment 2;

step two, adding 95% ethanol into the zedoary turmeric powder obtained in the step one until the ethanol content is not lower than 90%, refrigerating overnight at 1 ℃ for 12h, separating to obtain a filtrate I and a filter residue I, adding water into the filter residue I according to the material-liquid ratio of 1:20, extracting water at 55 ℃ for 1h multiplied by 3 times, combining and filtering to obtain a filtrate II and a filter residue II; the filtrate I can be used for extracting zedoary turmeric alkaloid and other substances;

step three, decocting and concentrating the filtrate II at 110 ℃ to 30% volume, intercepting the macromolecular polysaccharide of the filtrate II by a 30KDa ultrafiltration membrane, adding absolute ethyl alcohol into the filtrate until the content of the ethanol is 90%, stirring for 10min at 600r/min, standing for 2h at room temperature, and separating and taking precipitate to obtain micromolecular zedoary turmeric polysaccharide a;

step four, combining the filter residue II and the macromolecular polysaccharide obtained in the step three, adding malic acid until the pH is =4, heating to 55 ℃, hydrolyzing for 60min, cooling to room temperature, and filtering to obtain a filtrate III and a filter residue III; adding water into filter residue III according to a material-liquid ratio of 1:20, extracting with water at 65 ℃ for 2h, filtering at room temperature to obtain filtrate IV and filter residue IV, mixing filtrate III and filtrate IV, decocting at 110 ℃ and concentrating to 20% volume, adding absolute ethanol into the filtrate after 30KDa ultrafiltration membrane interception until the alcohol content is not lower than 90%, stirring at 600r/min for 10min, standing at room temperature for 2h, and separating and collecting precipitate to obtain small molecular zedoary turmeric polysaccharide b.

Example 9: another zedoary turmeric polysaccharide:

the first step and the third step are the same as the example 2;

step four, adding the extract into a 300L jacketed kettle for decoction and concentration, setting the decoction temperature to be 180 ℃, and finishing the decoction and concentration when the extract does not emit steam, so as to obtain a dry extract semi-finished product;

the fifth to eighth steps were the same as in example 2.

Example 10: another zedoary turmeric polysaccharide:

the first step and the fifth step are the same as the embodiment 2;

adding 95% ethanol into the zedoary turmeric dry paste until the ethanol content is 75%, refrigerating at 1 ℃ overnight for 12h, separating to obtain a filtrate I and a filter residue I, adding water into the filter residue I according to the material-liquid ratio of 1:20, extracting with water at 55 ℃ for 1h multiplied by 3 times, and mixing and filtering to obtain a filtrate II and a filter residue II; the filtrate I can be used for extracting zedoary turmeric alkaloid and other substances;

seventhly, decocting and concentrating the filtrate II at 110 ℃ to 30% volume, intercepting the macromolecular polysaccharide of the filtrate II by using a 30KDa ultrafiltration membrane, adding absolute ethyl alcohol into the filtrate until the content of the ethanol is 75%, stirring for 10min at 600r/min, standing for 2h at room temperature, and separating and taking precipitate to obtain micromolecular zedoary turmeric polysaccharide a;

step eight, combining the filter residue II and the macromolecular polysaccharide obtained in the step seven, adding malic acid until the pH is =4, heating to 55 ℃, hydrolyzing for 60min, cooling to room temperature, and filtering to obtain a filtrate III and a filter residue III; adding water into filter residue III according to a material-liquid ratio of 1:20, extracting with water at 65 ℃ for 2h, filtering at room temperature to obtain filtrate IV and filter residue IV, mixing filtrate III and filtrate IV, decocting at 110 ℃ and concentrating to 20% volume, adding absolute ethanol into the filtrate after 30KDa ultrafiltration membrane interception until the alcohol content is 75%, stirring at 600r/min for 10min, standing at room temperature for 2h, and separating and collecting precipitate to obtain small molecular weight zedoary polysaccharide b.

Example 11: another zedoary turmeric polysaccharide:

the first step and the fifth step are the same as the embodiment 2;

adding 95% ethanol into the zedoary turmeric dry paste until the ethanol content is 50%, refrigerating at 1 ℃ overnight for 12h, separating to obtain a filtrate I and a filter residue I, adding water into the filter residue I according to the material-liquid ratio of 1:20, extracting with water at 55 ℃ for 1h multiplied by 3 times, and mixing and filtering to obtain a filtrate II and a filter residue II; the filtrate I can be used for extracting zedoary turmeric alkaloid and other substances;

seventhly, decocting and concentrating the filtrate II at 110 ℃ to 30% volume, intercepting the macromolecular polysaccharide of the filtrate II by using a 30KDa ultrafiltration membrane, adding absolute ethyl alcohol into the filtrate until the content of the ethanol is 50%, stirring for 10min at 600r/min, standing for 2h at room temperature, and separating and taking precipitate to obtain micromolecular zedoary turmeric polysaccharide a;

step eight, combining the filter residue II and the macromolecular polysaccharide obtained in the step seven, adding malic acid until the pH is =4, heating to 55 ℃, hydrolyzing for 60min, cooling to room temperature, and filtering to obtain a filtrate III and a filter residue III; adding water into filter residue III according to a material-liquid ratio of 1:20, extracting with water at 65 ℃ for 2h, filtering at room temperature to obtain filtrate IV and filter residue IV, mixing filtrate III and filtrate IV, decocting at 110 ℃ and concentrating to 20% volume, adding absolute ethanol into the filtrate after 30KDa ultrafiltration membrane interception until the alcohol content is 50%, stirring at 600r/min for 10min, standing at room temperature for 2h, and separating and collecting precipitate to obtain small molecular weight zedoary polysaccharide b.

Example 12: another zedoary turmeric polysaccharide:

the first step to the seventh step are the same as those in the embodiment 2;

step eight, combining the filter residue and the macromolecular polysaccharide obtained in the step seven, adding citric acid until the pH is =4, heating to 55 ℃, hydrolyzing for 60min, cooling to room temperature, and filtering to obtain a filtrate III and a filter residue III; adding water into filter residue III according to a material-liquid ratio of 1:20, extracting with water at 65 ℃ for 2h, filtering at room temperature to obtain filtrate IV and filter residue IV, mixing filtrate III and filtrate IV, decocting at 110 ℃ and concentrating to 20% volume, adding absolute ethanol into the filtrate after 30KDa ultrafiltration membrane interception until the alcohol content is not lower than 90%, stirring at 600r/min for 10min, standing at room temperature for 2h, and separating and collecting precipitate to obtain small molecular zedoary turmeric polysaccharide b.

Example 13: another zedoary turmeric polysaccharide:

the first step to the seventh step are the same as those in the embodiment 2;

step eight, combining the filter residue II and the macromolecular polysaccharide obtained in the step seven, adding hydrochloric acid until the pH is =4, heating to 55 ℃, hydrolyzing for 60min, cooling to room temperature, and filtering to obtain a filtrate III and a filter residue III; adding water into filter residue III according to a material-liquid ratio of 1:20, extracting with water at 65 ℃ for 2h, filtering at room temperature to obtain filtrate IV and filter residue IV, mixing filtrate III and filtrate IV, decocting at 110 ℃ and concentrating to 20% volume, adding absolute ethanol into the filtrate after 30KDa ultrafiltration membrane interception until the alcohol content is not lower than 90%, stirring at 600r/min for 10min, standing at room temperature for 2h, and separating and collecting precipitate to obtain small molecular zedoary turmeric polysaccharide b.

Example 14: another zedoary turmeric polysaccharide:

the first step to the seventh step are the same as those in the embodiment 2;

step eight, combining the filter residue II and the macromolecular polysaccharide obtained in the step seven, adding hydrochloric acid until the pH is =2, heating to 55 ℃, hydrolyzing for 60min, cooling to room temperature, and filtering to obtain a filtrate III and a filter residue III; adding water into filter residue III according to a material-liquid ratio of 1:20, extracting with water at 65 ℃ for 2h, filtering at room temperature to obtain filtrate IV and filter residue IV, mixing filtrate III and filtrate IV, decocting at 110 ℃ and concentrating to 20% volume, adding absolute ethanol into the filtrate after 30KDa ultrafiltration membrane interception until the alcohol content is not lower than 90%, stirring at 600r/min for 10min, standing at room temperature for 2h, and separating and collecting precipitate to obtain small molecular zedoary turmeric polysaccharide b.

Experimental example 1: the yield of the zedoary turmeric polysaccharide is as follows:

the yields of the small molecule zedoaria polysaccharide a and the small molecule zedoaria polysaccharide b obtained in each of examples 2 to 14 and the yields thereof relative to the crude drugs were counted, and the statistical results are shown in table 3.

TABLE 3 polysaccharide content and yield

As can be seen from table 3, the small molecule zedoaria polysaccharide a and the small molecule zedoaria polysaccharide b in the preferred examples 2 and 12 of the present application each had a high extraction rate, which was more than 1.65% with respect to the total yield of crude drugs; as can be seen from the comparative examples 2 to 7, the extrusion and expansion pretreatment of the curcuma zedoaria is beneficial to the precipitation of the small molecular curcuma zedoaria polysaccharide b component, and meanwhile, the selection of the water content, the screw rotation speed and the extrusion temperature of the curcuma zedoaria medicinal material during the extrusion and expansion pretreatment has an important influence on the yield of the small molecular curcuma zedoaria polysaccharide b component; from examples 8 and 9, it can be seen that the scheme of extracting polysaccharides from Curcuma zedoaria powder without preparing dry extract is not favorable for obtaining polysaccharides, and the yield of total polysaccharides is not lower than 1%; in addition, the alcohol precipitation content of the extracted polysaccharide also affects the extraction rate of the polysaccharide, and it can be seen from comparative examples 13 and 14 that the substitution of a strong acid for malic acid or citric acid as described herein is not beneficial to the extraction of the polysaccharide, and even if the acidity is increased, a good extraction effect cannot be obtained.

It should be noted that the yield and yield of the kaempferia pandurata polysaccharide according to the present invention may vary from the prior art depending on the kaempferia pandurata field and/or variety, but the implementation of the present invention and the advancement of the present invention are not affected, because the skilled in the art can still know the advancement of the present invention by using the present invention to extract kaempferia pandurata from other sources and/or varieties.

Experimental example 2: detection of analgesic effect of zedoary turmeric polysaccharide:

animal model: taking 80 Wistar rats with similar ages and body weights, randomly dividing the Wistar rats into 8 groups, a blank group, a positive group and a drug group, wherein the drug group comprises a dry paste low dose group, a dry paste high dose group, a polysaccharide low dose group and a polysaccharide high dose group, the blank group is fed with normal saline, the positive group is fed with aspirin, the drug group is respectively fed with the dry paste and the polysaccharide (distinguishing micromolecule zedoary polysaccharide a and micromolecule zedoary polysaccharide b) in the embodiment 2, the dry paste and the polysaccharide are respectively dissolved in the normal saline, and the feeding doses of the positive group and the drug group are respectively as follows: 0.025g/kg of aspirin in a positive group, 0.1g/kg of a dry paste low dose group, 1g/kg of a dry paste high dose group, 0.01g/kg of a small molecule zedoary polysaccharide a low dose group, 0.1g/kg of a small molecule zedoary polysaccharide a high dose group, 0.01g/kg of a small molecule zedoary polysaccharide b low dose group and 0.1g/kg of a small molecule zedoary polysaccharide b low dose group; the doses were administered for three days, once a day, respectively.

And (3) detection: acetic acid induced rat pain response model, 0.6% acetic acid solution (0.2 mL/mouse) was injected intraperitoneally into each rat 30min after the last administration of the animals, and the number of writhing responses occurring and the time (latency) for the first writhing response occurring within 15min after acetic acid injection were observed and recorded. The statistical results are shown in table 4.

TABLE 4 analgesic Effect

As can be seen from table 4, both the kaempferia galamga dry paste and kaempferia galamga polysaccharide obtained by the technical scheme of the present application have excellent analgesic effects (acetic acid induced rat pain model), and particularly, the high-dose group of the small molecule kaempferia galamga polysaccharide b obtained by the technical scheme of the present application has better analgesic effects than the positive drug aspirin, which indicates that the characteristics and properties of the drugs are not damaged by the prepared dry paste and even the prepared polysaccharide, and the scheme of the present application provides a new direction for the kaempferia galamga analgesic research.

Experimental example 3: and (3) anti-aging effect detection:

animal model: kunming mice weighing about 30g are randomly divided into 8 groups, and each group comprises 10 mice (half a female and half): blank group, model group, lentinan group, zedoary polysaccharide group, wherein the zedoary polysaccharide group is further subdivided into 5 groups based on the polysaccharides obtained in example 2, and the groups are respectively: the weight ratio of the small molecule zedoary polysaccharide a group to the small molecule zedoary polysaccharide b is 1:1 group, the weight ratio of the small molecule zedoary polysaccharide a to the small molecule zedoary polysaccharide b is 9:1 group, the weight ratio of the small molecule zedoary polysaccharide a to the small molecule zedoary polysaccharide b is 4:1 low dose group, and the weight ratio of the small molecule zedoary polysaccharide a to the small molecule zedoary polysaccharide b is 4:1 high dose group; the components are as follows:

blank group: perfusing equal volume of normal saline for stomach, and marking as group B;

model group: injecting 120mg/kg D-galactose and normal saline with the same volume as the gavage into the neck and back part subcutaneously, and recording as group C;

lentinan group: injecting 120mg/kg of D-galactose and 100mg/kg of lentinan with the same volume as the gavage into the neck and back part subcutaneously, and recording as group M;

small molecule zedoary polysaccharide a group: injecting 120mg/kg of D-galactose and 50mg/kg of the small molecule zedoary turmeric polysaccharide a obtained in the example 2 with the same volume of intragastric injection into the neck and back part subcutaneously, and marking as a group a;

the weight ratio of the small molecular zedoary polysaccharide a to the small molecular zedoary polysaccharide b is 1: injecting 120mg/kg of D-galactose and 120mg/kg of small molecule zedoary turmeric polysaccharide a and 25mg/kg of small molecule zedoary turmeric polysaccharide b obtained in example 2 with the same volume of intragastric injection into the back of the neck, and recording as a + b (1:1) group;

the weight ratio of the small molecular zedoary polysaccharide a to the small molecular zedoary polysaccharide b is 9: 1: injecting 120mg/kg of D-galactose and 120mg/kg of small molecule zedoary turmeric polysaccharide and 5mg/kg of small molecule zedoary turmeric polysaccharide b obtained in the example 2 with the same volume of intragastric injection into the back of the neck, and recording the volume as a + b (9:1) group;

the weight ratio of the small molecular zedoary polysaccharide a to the small molecular zedoary polysaccharide b is 4:1, and the weight ratio is as follows: injecting 120mg/kg of D-galactose and 120mg/kg of small molecule zedoary turmeric polysaccharide and 10mg/kg of small molecule zedoary turmeric polysaccharide b obtained in example 2 with the same volume of intragastric injection into the back of the neck, and recording as a + b (4:1) -L group;

the weight ratio of the small molecular zedoary polysaccharide a to the small molecular zedoary polysaccharide b is 4:1, and the weight ratio is as follows: 120mg/kg of D-galactose and the same volume of the small molecule zedoary polysaccharide a 80mg/kg and the small molecule zedoary polysaccharide b 20mg/kg obtained in example 2 were injected subcutaneously into the back of the neck, and the injection was recorded as a + b (4:1) -H group.

The administration is carried out by gavage once a day for 60 days according to 0.2mL/10g respectively.

And (3) detection: referring to the prior art, the detection is carried out according to the following methods:

1 determination of mouse thymus and spleen indices: weighing the weight of the mice 24h after the last administration, killing the mice after blood is taken from the orbit, taking and weighing thymus, spleen, liver and brain tissues, and calculating thymus index and spleen index according to thymus (mg)/weight (g) and spleen (mg)/weight (g);

2, measuring the MDA content and the SOD activity of the blood of the mouse: immediately centrifuging the blood of the mouse to obtain the serum of the mouse, and measuring the MDA content and the SOD activity of the blood of the mouse according to the prior art;

3 mouse liver tissue GSH-Px activity determination: taking mouse liver, removing connective tissue, grinding by a homogenizer, and adding pre-cooled normal saline to prepare 10% tissue homogenate; freezing and centrifuging (2-4 ℃), taking supernatant, and measuring the activity of glutathione peroxidase by using a glutathione peroxidase kit;

4 mouse brain tissue CAT activity determination: taking a mouse brain tissue, adding pre-cooled physiological saline for homogenizing to prepare a 10% tissue homogenate; freezing and centrifuging (2-4 ℃), taking supernatant, and measuring the activity of catalase by using a catalase kit. The results are shown in FIG. 4 and Table 5.

TABLE 5 mouse blood, liver tissue and brain tissue viability

As can be seen from fig. 4 and table 5, the small molecule of the zedoary polysaccharide a obtained in example 2 of the present application alone did not significantly increase the thymus index and spleen index of the molded mice, and the blood, liver tissue and brain tissue viability of the mice did not significantly change compared to the D-galactose model group; the small molecular zedoary polysaccharide a and the small molecular zedoary polysaccharide b obtained in the embodiment 2 are compounded for use, so that an excellent anti-aging effect can be obtained, the thymus index and the spleen index of an aging molding mouse are obviously improved, the blood SOD activity, the liver tissue GSH-Px activity, the brain tissue CAT activity and the like of the mouse are improved to different degrees, and the blood MDA content is lower, so that the immune function of the body is favorably enhanced, the anti-oxidation capability of the body is improved, redundant free radicals are eliminated, and the aging is delayed.

Conventional techniques and schemes not described in detail in the above embodiments are well known in the art, and thus are not described in detail herein.

The preferred embodiments of the present invention have been described in detail by the above examples and/or experimental examples, however, the present invention is not limited to the specific details in the above embodiments, and various simple modifications may be made to the technical solution of the present invention within the technical idea of the present invention, and these simple modifications all fall within the protective scope of the present invention.

It should be noted that the various features described in the above embodiments may be combined in any suitable manner without departing from the scope of the invention. The invention is not described in detail in order to avoid unnecessary repetition.

In addition, any combination of the various embodiments of the present invention is also possible, and the same should be considered as the disclosure of the present invention as long as it does not depart from the spirit of the present invention.

The invention is not limited to the best of the technology known in the field.

Claims

1. The preparation method of the zedoary turmeric polysaccharide is characterized by comprising the following steps:

step two, stirring and concentrating the filtered liquid medicine to obtain extract;

adding 95% ethanol into the zedoary turmeric dry paste until the ethanol content is not lower than 90%, refrigerating overnight, separating to obtain a filtrate I and a filter residue I, adding water into the filter residue I for water extraction, and filtering at room temperature to obtain a filtrate II and a filter residue II; the filtrate I can be used for extracting zedoary turmeric alkaloid;

2. The method of claim 1, wherein: before the water is added for decoction, the method further comprises the step of carrying out extrusion and expansion pretreatment on the rhizoma zedoariae, and specifically comprises the steps of drying the rhizoma zedoariae to the water content of 15-18%, carrying out extrusion and expansion pretreatment by a double-screw extruder, and crushing to pass through a 100-mesh sieve.

3. The method of claim 2, wherein: the feeding speed of the double-screw extruder during extrusion and puffing pretreatment is 5-10 kg/h, the rotating speed of the screw is 300-600 r/min, and the extrusion temperature is 110-120 ℃.

4. The method of claim 1, wherein: in the fifth step, the ratio of the water to the material liquid is 1: 20-50, the water extraction temperature is 55-60 ℃, and the extraction is performed for 3 times (1-2 h).

5. The method of claim 1, wherein: and seventhly, the acid is citric acid or malic acid, the hydrolysis temperature is 55-60 ℃, and the hydrolysis time is 45-60 min.

6. The method of claim 1, wherein: and seventhly, adding water to carry out water extraction at the water extraction temperature of 60-65 ℃ for 2-3 h, wherein the ratio of the water to the material liquid is 1: 15-30.

7. Use of the Kaempferia pandurata dry paste obtained by the method of any one of claims 1 to 6 for preparing an analgesic preparation.

8. Use of the curaya polysaccharide obtained by the method of any one of claims 1 to 6 for the preparation of an analgesic preparation.

9. Use of the small molecule curdlan a and the small molecule curdlan b obtained by the method of any one of claims 1 to 6 for preparing an anti-aging agent.

10. Use according to claim 9, characterized in that: the anti-aging preparation comprises a small molecular zedoary turmeric polysaccharide a and a small molecular zedoary turmeric polysaccharide b in a weight ratio of 3-5: 1.