CN106942734A

CN106942734A - The extracting method of Momordica grosvenori active ingredient

Info

Publication number: CN106942734A
Application number: CN201710198080.XA
Authority: CN
Inventors: 何伟平
Original assignee: Guilin Strength Science And Technology Ltd
Current assignee: Guilin Strength Science And Technology Ltd
Priority date: 2017-03-29
Filing date: 2017-03-29
Publication date: 2017-07-14

Abstract

The present invention relates to the extracting method of technical field of plant extraction, more particularly to Momordica grosvenori active ingredient, including following preparation process：1) take Momordica grosvenori to crush, add the water of 7~11 times of Momordica grosvenori weight, Microwave Extraction obtains Microwave Extraction thing and Luohanguo's residue；2) Luohanguo's residue after Microwave Extraction is added to the ethanol solution of 6~10 times of its weight, ultrasonic wave extraction, ultrafiltration obtains ultrasonic wave extraction thing；3) by step 1) obtain ultrasonic wave extraction thing and step 2) obtain ultrasonic wave extraction thing be well mixed, vacuum-concentrcted, spray drying, you can obtain Momordica grosvenori active ingredient.The active ingredient of obtained Momordica grosvenori, which is prepared by the method for the present invention, to be had effects that more to significantly improve body immunity.

Description

The extracting method of Momordica grosvenori active ingredient

Technical field

The present invention relates to the extracting method of technical field of plant extraction, more particularly to Momordica grosvenori active ingredient.

Background technology

Momordica grosvenori, the fruit of the perennial liana of Curcurbitaceae.Alias LAHANGUO, balloonvine heartseed herb, Semen Momordicae, it is invaluable, Arhat table, naked tortoise bar, are described as " angle is really " by people, and its leaf is heart-shaped, dioecism, summer-flowering, seed in autumn.Mainly originate in Guangxi Zhuang Autonomous Region Guilin City Yongfu county Longjiang township, dragon victory and hundred Shou Deng towns, Yongfu county He Longsheng counties are township's kinds of Momordica grosvenori Plant history longer, wherein plantation Momordica grosvenori in Yongfu has had more than 300 years history, Long Sheng counties plantation Momordica grosvenori has had 200 History for many years, Chinese 90 percent Momordica grosvenori originates in Yongfu county He Longsheng counties, and Momordica grosvenori is the rare local and special products in Guilin, is also One of medicine-food two-purpose material that country ratifies in the first batch, its primary efficacy is can be relieving cough and reducing sputum.Fruits nutrition value is very high, containing abundant Vitamin C (in every 100 grams of fresh fruits contain 400 milligrams~500 milligrams) and glucoside, fructose, glucose, protein, lipid etc..

Momordica grosvenori is sweet cool in nature, return lung, large intestine channel, moistens the lung and relieve the cough, promotes the production of body fluid to quench thirst, suitable for lung heat or the dryness of the lung Cough, pertussis and hot summer weather fluid deficiency and thirsty etc., in addition with the effect relaxed bowel.Momordica grosvenori is the medicine that the Ministry of Public Health announces in the first batch Dual-purpose rare traditional Chinese medicine is eaten, 300 times sweeter than sucrose of its contained Momordica-Glycosides does not produce heat, is beverage, the name of confectionery industry Your raw material, is the best substitute of sucrose.Often drink " Luohan " fruit tea, can prevent a variety of diseases, modern medicine is proved, Momordica grosvenori is to branch gas The diseases such as Guan Yan, hypertension have significant curative effect, still play a part of prevention and treatment of coronary heart disease, vascular sclerosis, obesity.Nutritive value Height, the effects such as having clearing away summerheat, preventing phlegm from forming and stopping coughing, cool blood easypro bone, clearing lung-heat ease constipation and promote the production of body fluid to quench thirst；Can treating acute and chronic tracheitis, pharynx The diseases such as laryngitis, bronchial astehma, pertussis, stomach energy, constipation, acute tonsillitis, diabetic also preferably takes.

There are some researches show Momordica grosvenori is improved effect of body immunity, but this effect be not it is particularly pertinent, because The method that a kind of extraction arhat active ingredient of this research and development is used for improving body immunity is highly desirable to.

The content of the invention

The technical problems to be solved by the invention are to provide the extracting method of Momordica grosvenori active ingredient, and this method is prepared Momordica grosvenori active ingredient can effectively improve body immunity.

To achieve the above object, technical scheme to be solved by this invention is：

The present invention provides the extracting method of Momordica grosvenori active ingredient, including following preparation process：

1) take Momordica grosvenori to crush, add the water of 7~11 times of Momordica grosvenori weight, Microwave Extraction obtains Microwave Extraction thing and sieve Chinese pomace；

2) Luohanguo's residue after Microwave Extraction is added to the ethanol solution of 6~10 times of its weight, ultrasonic wave extraction surpasses Filter, obtains ultrasonic wave extraction thing；

3) by step 1) obtain Microwave Extraction thing and step 2) obtain ultrasonic wave extraction thing be well mixed, vacuum decompression Concentration, spray drying, you can obtain Momordica grosvenori active ingredient.

Step 1 of the present invention) in, mangosteen powder is broken to 100~200 mesh.Momordica grosvenori is crushed behind favourable Momordica grosvenori Extraction, shorten manufacturing cycle, improve active ingredient recovery rate.

Step 1 of the present invention) in, 30~45min of Microwave Extraction under 400~600W.Microwave Extraction mild condition, has It is not destroyed beneficial to the active ingredient of Momordica grosvenori is protected.

Step 1 of the present invention) in, temperature during Microwave Extraction is 45~55 DEG C.Temperature is suitable, is conducive to protecting arhat The activity of fruit active ingredient.

Step 2 of the present invention) in, the volumetric concentration of ethanol solution is 40~60%.Further expand Momordica grosvenori to extract The scope of thing, body immunity is improved to improve Momordica grosvenori active ingredient.

Step 2 of the present invention) in, 25~35min of ultrasonic wave extraction under 60~90KHz.Ultrasonic wave extraction condition temperature With, ensure recovery rate while, the bioactivity of Momordica grosvenori active ingredient can be effectively improved, it is ensured that the arhat prepared Berry extract bioactivity is high.

Step 2 of the present invention) in, temperature during ultrasonic wave extraction is 30~40 DEG C.Proper temperature, beneficial to active ingredient Dissolution.

Step 2 of the present invention) in, carry out ultrafiltration with molecular cut off for 80~900,000 seperation film.By such Filtering, can further improve the purity of Momordica grosvenori active ingredient, improve the bioactivity of Momordica grosvenori active ingredient, so that it is guaranteed that The Momordica grosvenori active ingredient prepared has the bioactivity for the body immunity that increases significantly.

Step 2 of the present invention) in, ultra-filtration conditions are 350~450r/min of mixing speed, 0.6~0.9MPa of pressure.Limit Determine the condition of ultrafiltration, be conducive to improving the effect of ultrafiltration.

Step 3 of the present invention) in, it is 1.12~1.18 to be concentrated into relative density.Concentration is conducive to drying below, and Drying time can be shortened.

Active ingredient in Momordica grosvenori is extracted by the inventive method, Microwave Extraction and ultrasonic wave extraction, condition has been selected Gently, the active ingredient of the Momordica grosvenori prepared from different solvents, such method, which is had, more significantly improves human body Effect of immunity.

Embodiment

The invention will be further described with reference to embodiments, but the invention is not limited in these embodiments.

Embodiment 1

1) take mangosteen powder to be broken to 100 mesh, add the water of 1 times of Momordica grosvenori weight, microwave is carried at a temperature of 400W and 55 DEG C 45min is taken, Microwave Extraction thing and Luohanguo's residue is obtained；

2) Luohanguo's residue after Microwave Extraction is added into the ethanol solution that 10 times of volumetric concentrations of its weight are 40%, Ultrasonic wave extraction 30min at 90KHz and 30 DEG C, carries out ultrafiltration, ultra-filtration conditions are to stir with molecular cut off for 800,000 seperation film Speed 350r/min, pressure 0.6MPa are mixed, ultrasonic wave extraction thing is obtained；

3) by step 1) obtain Microwave Extraction thing and step 2) obtain ultrasonic wave extraction thing be well mixed, vacuum decompression It is 1.12 to be concentrated into relative density, spray drying, you can obtain Momordica grosvenori active ingredient.

Embodiment 2

1) take mangosteen powder to be broken to 120 mesh, add the water of 10 times of Momordica grosvenori weight, microwave is carried at a temperature of 450W and 45 DEG C 30min is taken, Microwave Extraction thing and Luohanguo's residue is obtained；

2) Luohanguo's residue after Microwave Extraction is added into the ethanol solution that 9 times of volumetric concentrations of its weight are 45%, Ultrasonic wave extraction 25min at 80KHz and 35 DEG C, carries out ultrafiltration, ultra-filtration conditions are to stir with molecular cut off for 820,000 seperation film Speed 450r/min, pressure 0.7MPa are mixed, ultrasonic wave extraction thing is obtained；

3) by step 1) obtain Microwave Extraction thing and step 2) obtain ultrasonic wave extraction thing be well mixed, vacuum decompression It is 1.18 to be concentrated into relative density, spray drying, you can obtain Momordica grosvenori active ingredient.

Embodiment 3

1) take mangosteen powder to be broken to 150 mesh, add the water of 9 times of Momordica grosvenori weight, microwave is carried at a temperature of 500W and 55 DEG C 35min is taken, Microwave Extraction thing and Luohanguo's residue is obtained；

2) Luohanguo's residue after Microwave Extraction is added into the ethanol solution that 8 times of volumetric concentrations of its weight are 50%, Ultrasonic wave extraction 35min at 70KHz and 40 DEG C, carries out ultrafiltration, ultra-filtration conditions are to stir with molecular cut off for 850,000 seperation film Speed 450r/min, pressure 0.8MPa are mixed, ultrasonic wave extraction thing is obtained；

3) by step 1) obtain Microwave Extraction thing and step 2) obtain ultrasonic wave extraction thing be well mixed, vacuum decompression It is 1.16 to be concentrated into relative density, spray drying, you can obtain Momordica grosvenori active ingredient.

Embodiment 4

1) take mangosteen powder to be broken to 180 mesh, add the water of 8 times of Momordica grosvenori weight, microwave is carried at a temperature of 600W and 50 DEG C 40min is taken, Microwave Extraction thing and Luohanguo's residue is obtained；

2) Luohanguo's residue after Microwave Extraction is added into the ethanol solution that 7 times of volumetric concentrations of its weight are 55%, Ultrasonic wave extraction 30min at 65KHz and 35 DEG C, carries out ultrafiltration, ultra-filtration conditions are to stir with molecular cut off for 880,000 seperation film Speed 400r/min, pressure 0.9MPa are mixed, ultrasonic wave extraction thing is obtained；

3) by step 1) obtain Microwave Extraction thing and step 2) obtain ultrasonic wave extraction thing be well mixed, vacuum decompression It is 1.14 to be concentrated into relative density, spray drying, you can obtain Momordica grosvenori active ingredient.

Embodiment 5

1) take mangosteen powder to be broken to 200 mesh, add the water of 7 times of Momordica grosvenori weight, microwave is carried at a temperature of 550W and 45 DEG C 45min is taken, Microwave Extraction thing and Luohanguo's residue is obtained；

2) Luohanguo's residue after Microwave Extraction is added into the ethanol solution that 6 times of volumetric concentrations of its weight are 60%, Ultrasonic wave extraction 25min at 60KHz and 30 DEG C, carries out ultrafiltration, ultra-filtration conditions are to stir with molecular cut off for 900,000 seperation film Speed 350r/min, pressure 0.6MPa are mixed, ultrasonic wave extraction thing is obtained；

Comparative example 1

1) take mangosteen powder to be broken to 150 mesh, add the water of 9 times of Momordica grosvenori weight, microwave is carried at a temperature of 500W and 55 DEG C 35min is taken, Microwave Extraction thing is obtained, vacuum-concentrcted to relative density is 1.16, spray drying, you can obtaining Momordica grosvenori has Imitate composition.

Comparative example 2

1) take mangosteen powder to be broken to 150 mesh, add the ethanol solution that 8 times of volumetric concentrations of its weight are 50%, in 70KHz and Ultrasonic wave extraction 35min at 40 DEG C, carries out ultrafiltration, ultra-filtration conditions are mixing speed with molecular cut off for 850,000 seperation film 450r/min, pressure 0.8MPa, obtain ultrasonic wave extraction thing；

2) by step 2) obtain ultrasonic wave extraction thing be well mixed, vacuum-concentrcted to relative density be 1.16, spray Mist is dried, you can obtain Momordica grosvenori active ingredient.

Comparative example 3

Take mangosteen powder to be broken to 150 mesh, add the water of 9 times of Momordica grosvenori weight, decoct 60min, filtering, vacuum-concentrcted It is 1.16 to relative density, spray drying, you can obtain Momordica grosvenori active ingredient.

Test example 1：Influence experiment of the Momordica grosvenori active ingredient that the inventive method is prepared to immunity

1.1st, experimental animal：Mouse is randomly divided into 5 groups by 100 healthy Kunming mouses, male and female half and half, 18~22g： Blank group (20), 3 groups of embodiment (20), 1 group of comparative example (20), 2 groups of comparative example (20), 3 groups of comparative example (20).

1.2nd, experimental method：0.2g/kg extract is given by treatment group, and (embodiment of the present invention 3, comparative example 1~3 are prepared into Arrive, be diluted with water to content of dispersion for 0.1g/ml) gastric infusion, control group carries out gavage using same amount of physiological saline.Daily Gavage 1 time, continuous gavage 4 weeks.After each group mouse last gavage 60min.From mouse tail vein injection prepared Chinese ink 0.1ml/10g, in 2min, 12min after injection, take blood from retroorbital venous clump respectively, after conversion, obtain phagocytic index.Thymus gland and spleen is taken to claim it Quality simultaneously calculates itself and body weight ratio respectively, is corresponding mouse immune shoot formation, specific experiment result is as shown in table 1：

The each group mouse immune shoot formation of table 1 and phagocytic index

Group	Spleen index	Thymus gland refers to	Phagocytic index
				Control group	0.017±0.003	0.019±0.002	0.019±0.002
3 groups of embodiment	0.025±0.003	0.026±0.002	0.026±0.001
				1 group of comparative example	0.020±0.003	0.021±0.002	0.021±0.003
2 groups of comparative example	0.021±0.003	0.022±0.002	0.022±0.002
				3 groups of comparative example	0.019±0.003	0.020±0.002	0.020±0.003

Shown by the result of the test of table 1, the Momordica grosvenori active ingredient phagocytic index that the inventive method is prepared higher than pair According to group and comparative example group, and the thymus gland coefficient and Spleen coefficient of immune organ are also above control group and comparative example group, show this The Momordica grosvenori active ingredient that inventive method is prepared has effects that more to significantly improve body immunity.

Claims

1. the extracting method of Momordica grosvenori active ingredient, it is characterised in that including following preparation process：

1) take Momordica grosvenori to crush, add the water of 7~11 times of Momordica grosvenori weight, Microwave Extraction obtains Microwave Extraction thing and Momordica grosvenori Slag；

2) Luohanguo's residue after Microwave Extraction is added to the ethanol solution of 6~10 times of its weight, ultrasonic wave extraction, ultrafiltration is obtained To ultrasonic wave extraction thing；

3) by step 1) obtain Microwave Extraction thing and step 2) obtain ultrasonic wave extraction thing be well mixed, vacuum decompression is dense Contracting, spray drying, you can obtain Momordica grosvenori active ingredient.

2. the extracting method of Momordica grosvenori active ingredient according to claim 1, it is characterised in that：The step 1) in, sieve Chinese fruit powder is broken to 100~200 mesh.

3. the extracting method of Momordica grosvenori active ingredient according to claim 1, it is characterised in that：The step 1) in, 30~45min of Microwave Extraction under 400~600W.

4. the extracting method of Momordica grosvenori active ingredient according to claim 3, it is characterised in that：The step 1) in, it is micro- Temperature when ripple is extracted is 45~55 DEG C.

5. the extracting method of Momordica grosvenori active ingredient according to claim 1, it is characterised in that：The step 2) in, second The volumetric concentration of alcoholic solution is 40~60%.

6. the extracting method of Momordica grosvenori active ingredient according to claim 1, it is characterised in that：The step 2) in, 25~35min of ultrasonic wave extraction under 60~90KHz.

7. the extracting method of Momordica grosvenori active ingredient according to claim 6, it is characterised in that：The step 2) in, surpass Temperature when sound wave is extracted is 30~40 DEG C.

8. the extracting method of Momordica grosvenori active ingredient according to claim 7, it is characterised in that：The step 2) in, use Molecular cut off carries out ultrafiltration for 80~900,000 seperation film.

9. the extracting method of Momordica grosvenori active ingredient according to claim 8, it is characterised in that：The step 2) in, surpass Filter condition is 350~450r/min of mixing speed, 0.6~0.9MPa of pressure.

10. the extracting method of Momordica grosvenori active ingredient according to claim 1, it is characterised in that：The step 3) in, it is dense It is 1.12~1.18 to be reduced to relative density.