CN112245363A - Salvia miltiorrhiza whitening and freckle removing mask - Google Patents
Salvia miltiorrhiza whitening and freckle removing mask Download PDFInfo
- Publication number
- CN112245363A CN112245363A CN202011310970.3A CN202011310970A CN112245363A CN 112245363 A CN112245363 A CN 112245363A CN 202011310970 A CN202011310970 A CN 202011310970A CN 112245363 A CN112245363 A CN 112245363A
- Authority
- CN
- China
- Prior art keywords
- extract
- whitening
- salvia miltiorrhiza
- red sage
- sage root
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P29/00—Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q17/00—Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
- A61Q17/005—Antimicrobial preparations
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/52—Stabilizers
- A61K2800/522—Antioxidants; Radical scavengers
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/59—Mixtures
- A61K2800/592—Mixtures of compounds complementing their respective functions
- A61K2800/5922—At least two compounds being classified in the same subclass of A61K8/18
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/805—Corresponding aspects not provided for by any of codes A61K2800/81 - A61K2800/95
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Dermatology (AREA)
- Medicinal Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Pain & Pain Management (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Organic Chemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- Rheumatology (AREA)
- Biotechnology (AREA)
- Botany (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Birds (AREA)
- Epidemiology (AREA)
- Cosmetics (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses a whitening and freckle-removing mask containing salvia miltiorrhiza. Mixing water, butanediol, glycerol, dipropylene glycol, diglycerol, sucrose, polysorbate-20, phenoxyethanol, polyethylene glycol-32, methyl hydroxybenzoate, carbomer, sodium hydroxide, daily essence, Saviae Miltiorrhizae radix extract, flos Lonicerae extract and fructus Trichosanthis extract to obtain facial mask solution, and filling on facial mask cloth to obtain the final product. According to the invention, by optimizing the extraction factors, the extraction rates of salvianolic acid B in the salvia miltiorrhiza, chlorogenic acid in honeysuckle and polyphenol in the trichosanthes kirilowii maxim are improved, so that the salvia miltiorrhiza, the honeysuckle and the trichosanthes kirilowii maxim can be utilized to the maximum extent. The facial mask disclosed by the invention has the effects of whitening, moisturizing, removing freckles, diminishing inflammation and the like, is pure natural, non-toxic and non-irritant, and the natural product is applied to cosmetics, so that the application range of the traditional Chinese medicine is widened, and new vitality is also injected into the cosmetic industry.
Description
Technical Field
The invention relates to the technical field of cosmetics, and particularly relates to a red sage root whitening and freckle removing mask.
Background
The facial mask is an external film agent for nourishing and protecting facial skin, is convenient to use, and is often used as a cosmetic for facial beautification. By adding the raw materials with different functional components, the facial mask can make the skin moist, smooth and elastic after being applied for a period of time, achieves the effects of whitening and tendering the skin, and can also treat certain skin diseases. At present, common raw materials of the mask comprise flour, honey, eggs and beans which are frequently eaten by people, and common traditional Chinese medicines such as narcissus bulb, oxhorn powder, calamus root, kudzu root and the like.
With the increasing living standard of people, women and even men pay more and more attention to skin care, and in daily use of skin care products, the mask product is in the front. The facial mask has the characteristics of convenient carrying, simple and quick use and long-term storage, and becomes the most popular facial mask for consumers. The data show that more than 80 percent of the facial masks sold in the market at present are facial masks, and the facial masks mainly take non-woven base cloth as a carrier, so that the absorption speed and the absorption amount of the skin to nutrient components in nutrient solution can be improved. Meanwhile, the efficacy of the facial mask is a point of great importance when people buy facial mask products. The whitening and moisturizing facial masks are more in demand than other facial masks, and the effective components of the whitening facial masks which are popular in the market can prevent the formation of melanin.
The development of the mask industry is leaps and bounds in recent years, more than 300 mask brands exist in domestic markets, the permeability of mask users in China is about 45%, the market scale is about 100 million yuan, and the mask is growing at the speed of about 30% every year. For consumers, purchasing mask products no longer only pays attention to price, but tends to be rational and more concerned about safety. Therefore, natural safety will be the dominant development of the mask industry for the future mask market.
The traditional Chinese medicine has the advantages of obvious curative effect, mild effect, small toxic and side effects and the like, and the traditional Chinese medicine facial mask prepared by adding the traditional Chinese medicine components into the human facial mask has obvious curative effect on facial whitening and moisturizing. With the continuous improvement of the cosmetic market, the cosmetics containing the raw materials of medicinal plant extracts must meet new opportunities and develop. The traditional Chinese medicine cosmetic is a new field which is developed in China, even Japan and Korea. Chinese herbal medicine resources are rich in China, the theory of traditional Chinese medicine is profound and long in history, and the application of the functional components of the Chinese herbal medicine raw materials in cosmetics brings new vitality to the cosmetic industry. The method has an absolute advantage in mutual competition with Korea and Japan. Under the background of this big era, the development of new traditional Chinese medicine makeup skin care products is urgently needed.
Disclosure of Invention
Aiming at the prior art, the invention aims to provide a salvia miltiorrhiza whitening and freckle removing mask. The facial mask disclosed by the invention has the effects of whitening, moisturizing, removing freckles, diminishing inflammation and the like, is pure natural, non-toxic and non-irritant, and the natural product is applied to cosmetics, so that the application range of the traditional Chinese medicine is widened, and new vitality is also injected into the cosmetic industry.
In order to achieve the purpose, the invention adopts the following technical scheme:
in a first aspect of the invention, a red sage root whitening and freckle removing composition is provided, which comprises a red sage root extract, a honeysuckle flower extract and a trichosanthes kirilowii maxim extract; the mass ratio of the salvia miltiorrhiza extract to the honeysuckle flower extract to the trichosanthes kirilowii maxim extract is 1:1: 1.
Preferably, the salvia miltiorrhiza extract is prepared by the following method: mixing the salvia miltiorrhiza with deionized water, soaking, decocting and extracting for 2 times, combining filtrates, concentrating under reduced pressure until the relative density is 1.18-1.22, and vacuum drying to obtain the salvia miltiorrhiza extract.
Preferably, the mass ratio of the lead to the deionized water is 1: 12; decocting and extracting for 2 hours each time; the temperature of the reduced pressure concentration was 60 ℃.
Preferably, the honeysuckle flower extract is prepared by the following method: mixing flos Lonicerae with 8 mass times of deionized water, extracting at 90 deg.C for 1 hr, filtering, adding 6 mass times of deionized water into the residue, extracting at 90 deg.C for 1 hr, filtering, mixing filtrates, centrifuging, filtering to obtain flos Lonicerae extract, concentrating, and freeze drying to obtain flos Lonicerae extract.
Preferably, the trichosanthes kirilowii maxim extract is prepared by the following method: mixing fructus Trichosanthis with 40% ethanol water solution 8 times by mass, extracting at 60 deg.C for 1h, extracting for 2 times, mixing extractive solutions, recovering ethanol, centrifuging, filtering to obtain fructus Trichosanthis extractive solution, concentrating, and vacuum drying under reduced pressure to obtain fructus Trichosanthis extract.
The second aspect of the invention provides application of the salvia miltiorrhiza whitening and freckle removing composition in preparing whitening and freckle removing products.
The red sage root whitening and freckle removing composition can be used for preparing red sage root whitening and freckle removing skin care products, such as: the red sage root whitening and freckle removing liquid, the red sage root whitening and freckle removing mask and the like.
The third aspect of the invention provides a radix salviae miltiorrhizae whitening and freckle removing mask which takes the radix salviae miltiorrhizae whitening and freckle removing composition as an effective component.
Preferably, the salvia miltiorrhiza whitening and freckle removing mask comprises the following raw materials in percentage by weight: 6-8% of butanediol, 3-5% of glycerol, 3-5% of dipropylene glycol, 0.5% of diglycerol, 0.5% of sucrose, 200.4% of polysorbate, 0.25% of phenoxyethanol, 320.2% of polyethylene glycol, 0.2% of methylparaben, 0.086% of carbomer, 0.01% of sodium hydroxide, 0.05% of daily essence, 0.1% of salvia miltiorrhiza extract, 0.1% of honeysuckle flower extract, 0.1% of trichosanthes kirilowii maxim extract and the balance of water.
The fourth aspect of the invention provides a method for whitening and spot-removing the salvia miltiorrhiza mask, which comprises the following steps:
(1) mixing water, butanediol, glycerol, dipropylene glycol, diglycerol, sucrose, polyethylene glycol-32 and carbomer, heating to 80-85 ℃, homogenizing and stirring uniformly;
(2) adding Saviae Miltiorrhizae radix extract, flos Lonicerae extract and fructus Trichosanthis extract when the temperature is reduced to 50 deg.C, and stirring;
(3) after the temperature is reduced to 40 ℃, adding polysorbate-20, phenoxyethanol, methylparaben, sodium hydroxide and daily essence, and uniformly stirring to obtain the salvia miltiorrhiza whitening and freckle removing liquid;
(4) and (4) filling the red sage root whitening and freckle removing liquid obtained in the step (3) into a bag filled with a mask cloth to obtain the red sage root whitening and freckle removing mask.
The application method of the mask disclosed by the invention comprises the following steps:
(1) after cleaning the face, the mask is opened, the lining film is held by two hands, and the mask is lightly applied to the face with the face facing inwards.
(2) After the light pressure is uniform, the lining film is removed, and the film cloth is finely smoothed to be seamlessly attached to the outline of the face.
(3) The mask was removed after 15 minutes.
The invention has the beneficial effects that:
(1) according to the invention, the salvia miltiorrhiza extract, the honeysuckle extract, the trichosanthes kirilowii maxim extract and the like are prepared into the mask, and the salvia miltiorrhiza extract, the honeysuckle extract and the trichosanthes kirilowii maxim extract have synergistic effect, so that the mask not only can effectively whiten skin, but also has the effects of oxidation resistance, inflammation diminishing, bacteria resistance and the like, and can be used for sensitive skin. The invention has reasonable formula, natural and non-toxic raw materials and obvious effect. Is suitable for people of any age.
(2) According to the invention, by optimizing the extraction factors, the extraction rates of salvianolic acid B in the salvia miltiorrhiza, chlorogenic acid in honeysuckle and polyphenol in the trichosanthes kirilowii maxim are improved, so that the salvia miltiorrhiza, the honeysuckle and the trichosanthes kirilowii maxim can be utilized to the maximum extent.
(3) The facial mask disclosed by the invention has the effects of whitening, moisturizing, removing freckles, diminishing inflammation and the like, is pure natural, non-toxic and non-irritant, and the natural product is applied to cosmetics, so that the application range of the traditional Chinese medicine is widened, and new vitality is also injected into the cosmetic industry.
Drawings
FIG. 1 shows the results of the inhibition effect test of tyrosinase activity of 7 facial mask solutions.
Detailed Description
It should be noted that the following detailed description is exemplary and is intended to provide further explanation of the disclosure. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs.
As introduced in the background section, the whitening and spot-removing cosmetics achieve the whitening and spot-removing effects mainly through the following aspects: inhibiting melanin production in a cell by inhibiting tyrosinase or an intermediate for melanin production; or reducing melanin by stimulating keratinocyte to reduce melanin; reducing melanogenesis extracellularly by inhibiting endothelin-1 and endothelin-2; or by reducing the irradiation of ultraviolet rays. Inhibition of tyrosinase is still the main direction of development of whitening cosmetics among many methods. The tyrosinase inhibitor can inhibit tyrosinase activity or interfere melanin generation intermediate to prevent melanin generation, thereby achieving whitening and speckle removing effects. At present, the application of the active ingredients of the salvia miltiorrhiza in the field of cosmetics mainly lies in the aspects of oxidation resistance, antibiosis, inflammation diminishing, acne resistance, aging resistance and the like. Flos Lonicerae has antibacterial, antiinflammatory, antioxidant, and Trichosanthes kirilowii Maxim has antiinflammatory, antibacterial, antioxidant, and immunity improving effects. However, the combination of the three has not been reported to be used for whitening and removing freckles.
Based on the above, the invention aims to provide the salvia miltiorrhiza whitening and freckle removing mask. The salvia miltiorrhiza extract, the honeysuckle flower extract and the trichosanthes kirilowii maxim extract are added into the formula, and the salvia miltiorrhiza extract can inhibit the generation of melanin; meanwhile, the salvia miltiorrhiza can accelerate the removal of melanin by improving microcirculation. Helping the skin to regain homeostasis from two aspects. And experiments show that the inhibition effect of the total salvianolic acid on tyrosinase belongs to a reversible process. The total salvianolic acid causes the enzyme activity to be reduced by reducing the catalytic efficiency of tyrosinase, but not causes the enzyme activity to be reduced by reducing the effective enzyme amount. Therefore, the whitening of the salvia miltiorrhiza bunge basically has no irreversible side effect, and the salvia miltiorrhiza bunge can be recovered after being stopped if the salvia miltiorrhiza bunge is not suitable for use. The honeysuckle flower and the trichosanthes kirilowii are good medicines for resisting inflammation and relieving pain, and the anti-inflammatory effect of the honeysuckle flower and the trichosanthes kirilowii is proved by modern researches to have positive effects on relieving, repairing sensitive skin and improving whelk and allergic symptoms; reducing fine red blood filaments and adjusting uneven skin color; improving skin chapping and water shortage, balancing oil secretion, and enhancing skin moisture retention; reduce red hair and make hair brighter and lustrous. Especially, the honeysuckle flower has the effects of diminishing inflammation, resisting virus and resisting vascular proliferation, and can be applied to anti-sensitive high-end skin care products by applying a natural product extraction technology. And the salvia miltiorrhiza, the honeysuckle and the trichosanthes kirilowii have the antioxidation function. Therefore, when the salvia miltiorrhiza bunge is used as a raw material of the whitening cosmetics, water is used as an extraction solvent, which is beneficial to extracting effective components and more economical. The method has certain guiding significance for actual production.
Therefore, the invention adopts water extraction, the extraction rate of the salvianolic acid B is taken as an evaluation index, and the optimal extraction condition is obtained by setting extraction factors through experiment 1.
Test 1: extraction of active components from red sage root
1.1. The method for measuring the salvianolic acid B comprises the following steps:
content determination under Saviae Miltiorrhizae radix item in pharmacopoeia of people's republic of China.
1.2. Selection of extraction conditions
The invention preliminarily sets the extraction condition as water decoction extraction, selects the water adding amount, the extraction time and the extraction frequency as investigation factors, selects the salvianolic acid B extraction rate as investigation index, sets blank groups at the same time, and designs a four-factor three-level orthogonal test optimal water extraction process. The test was performed according to the orthogonal design table, the factor levels are shown in Table 1, the experimental arrangement and results are shown in Table 2, and the results of the ANOVA are shown in Table 3
TABLE 1 factor level table
TABLE 2 orthogonal experimental arrangement and results of extraction process
TABLE 3 analysis of variance
As shown in Table 2, the main and secondary actions of the factors are B & gtA & gtC, the analysis of variance shows that the influence of the B factors has significant difference (P & lt 0.05), and the optimal experimental scheme is A3B2C3Namely, the preferred process is to add 12 times of water and extract for 2 times with 2 hours each time.
1.3. Water extraction process parameter verification test
In order to further verify the optimal process conditions, three verification tests are carried out according to the orthogonal test results and the optimized process parameters, and the verification results are shown in the table 4:
table 4 water extraction optimum process validation results
The results of three tests show that the optimized process is stable and feasible and has good repeatability.
The water extraction of the honeysuckle flower and the trichosanthes kirilowii maxim has antioxidant activity, and the research shows that the antioxidant activity and the bacteriostatic activity of the water extraction of the honeysuckle flower and the trichosanthes kirilowii maxim are stronger than those of the ethanol extract. Therefore, the honeysuckle flower extract and the trichosanthes kirilowii maxim extract are extracted by water. The optimum extraction conditions were obtained by setting the extraction factors through experiment 2, with the total flavone content as the evaluation index.
Test 2: extraction of main effective component of honeysuckle flower
The main effective component of the honeysuckle flower is chlorogenic acid which is water-soluble organic acid, and meanwhile, the effective components of luteolin, quercetin and triterpenoid saponin can also be obtained by water extraction, so the product is selected by a water extraction process. Chlorogenic acid is selected as an evaluation index.
2.1 chlorogenic acid determination method:
the determination is carried out according to the specification of chlorogenic acid in GB/T22250-.
2.2 selection of extraction conditions
2.2.1 extraction method
The main factors influencing the content of chlorogenic acid include the feed-liquid ratio, the extraction temperature and the extraction time, and orthogonal experiments are carried out according to the level of 4 factors and 3 factors. The orthogonal experimental factors and levels are shown in table 5. The content of chlorogenic acid in the obtained sample extract was determined by the method of 3.1, and the results are shown in Table 6.
TABLE 5 orthogonal experimental factors and levels
TABLE 6 orthogonal experimental arrangement and results of extraction Process
TABLE 7 analysis of variance
As can be seen from the results in Table 7, the main action and the secondary action of each factor are B > C > A > D, i.e., the extraction temperature > the extraction time > the extraction times > the multiple of the solvent. Analysis of variance can find that the influence of the factor B has significance. The optimal extraction scheme is A2B2C3D1The preferable process is that the extraction times are 2 times, the extraction temperature is 90 ℃, the extraction time is 2 hours, and the solvent multiple is 14 times.
2.2.2 extraction Process parameter verification test
In order to further verify the optimal process conditions, three verification tests are carried out according to the orthogonal test results and the optimized process parameters. Extraction process parameters: the extraction temperature of the honeysuckle flower is 90 ℃, the extraction is carried out for 2 times, 8 times of water is added for extraction for 1 hour for the 1 st time, and 6 times of water is added for extraction for 1 hour for the 2 nd time. The results of the verification are shown in Table 8:
TABLE 8 verification of the optimal Process for chlorogenic acid
The results of three tests show that the optimized process is stable and feasible and has good repeatability.
2.3 honeysuckle flower extract technology:
extracting at 90 deg.C for 2 times, adding 8 times of water for 1 hr for 1 time, adding 6 times of water for 1 hr for 2 times, filtering, mixing filtrates, centrifuging, filtering to obtain flos Lonicerae extract, concentrating, and freeze drying to obtain flos Lonicerae extract lyophilized powder.
Test 3: extraction of main effective component of trichosanthes kirilowii
The fructus Trichosanthis contains polyphenols as main effective components, and can be dissolved in water or alcohol, and the solvent extraction method mainly comprises water solvent extraction and organic solvent extraction, so the product is extracted by alcohol-water mixed extraction process. Polyphenol is selected as an evaluation index.
3.1 Polyphenol determination method:
the determination was carried out according to Folin-Ciocalteu colorimetry.
3.2 selection of extraction conditions
3.2.1 extraction method
The main factors influencing the polyphenol content comprise a feed-liquid ratio, an extraction temperature, an extraction time and an ethanol concentration, and orthogonal experiments are carried out according to a 4-factor 3 level. The orthogonal experimental factors and levels are shown in Table 9. The content of polyphenol in the obtained sample extract was measured by the method in 3.1, and the results are shown in Table 10.
TABLE 9 orthogonal experimental factors and levels
TABLE 10 orthogonal experimental arrangement and results of extraction Process
TABLE 11 analysis of variance
As can be seen from the results in Table 11, the main effects of the factors are A > B > D > C, i.e., ethanol concentration > extraction temperature > solvent times > extraction time. The optimal extraction scheme is A1B2C3D3Namely, the preferable process is that the ethanol concentration is 40%, the extraction temperature is 60 ℃, the extraction time is 2h, and the solvent multiple is 16 times.
3.2.2 extraction Process parameter verification test
In order to further verify the optimal process conditions, three verification tests are carried out according to the orthogonal test results and the optimized process parameters. Extraction process parameters: the concentration of ethanol extracted from the trichosanthes kirilowii maxim is 40%, the extraction temperature is 60 ℃, the extraction time is 2 hours, and the solvent multiple is 16 times. The results of the verification are shown in Table 12:
TABLE 12 optimal Process validation of polyphenols
The results of three tests show that the optimized process is stable and feasible and has good repeatability.
3.3 Trichosanthes kirilowii Maxim extract process:
extracting at 60 deg.C for 2 times, respectively extracting with 8 times of 40% ethanol for 1 hr, mixing, recovering ethanol, centrifuging, filtering to obtain fructus Trichosanthis extract, concentrating, and vacuum drying under reduced pressure to obtain fructus Trichosanthis extract.
Test 4
The mask base had an influence on the final mask quality and the like, and the inventors selected an appropriate mask base by designing a one-factor test using the fragrance, the storage time, and the comfort as evaluation indexes, and obtained the results shown in table 13.
Watch 13
As is clear from Table 9, the substrate 4 was selected as the final film-forming substrate because the substrate 4 exhibited the best effect.
In order to make the technical solutions of the present application more clearly understood by those skilled in the art, the technical solutions of the present application will be described in detail below with reference to specific embodiments.
The test materials used in the examples of the present invention are all conventional in the art and commercially available.
Example 1
Preparation of Salvia miltiorrhiza extract
Soaking 12 times of radix salviae miltiorrhizae in deionized water by mass for 30min, decocting and extracting for 2 times, extracting for 2 hours for the first time and extracting for 2 hours for the second time, combining filtrates, concentrating under reduced pressure at 60 ℃ until the relative density is 1.18-1.22, and drying under vacuum to obtain the radix salviae miltiorrhizae extract.
Example 2
Honeysuckle flower extract
Extracting at 90 deg.C for 2 times, extracting flos Lonicerae 1 time with 8 mass times of deionized water for 1 hr, extracting residue of the extraction 2 times with 6 mass times of deionized water for 1 hr, filtering, mixing filtrates, centrifuging, filtering to obtain flos Lonicerae extract, concentrating the extract, and freeze drying to obtain flos Lonicerae extract lyophilized powder.
Example 3
Preparation method of Trichosanthes kirilowii Maxim extract
Extracting at 60 deg.C for 2 times, adding 8 times of 40% ethanol water solution, extracting for 1 hr, mixing, recovering ethanol, centrifuging, filtering to obtain fructus Trichosanthis extract, concentrating, and vacuum drying under reduced pressure to obtain fructus Trichosanthis extract.
Example 4
Preparation of whitening and freckle-removing mask with salvia miltiorrhiza
(1) Mixing 84.504g of water, 7g of butanediol, 3g of glycerol, 3g of dipropylene glycol, 0.5g of diglycerol, 0.5g of sucrose, 0.2g of polyethylene glycol-32 and 0.086g of carbomer, heating to 80-85 ℃, homogenizing and uniformly stirring;
(2) after the temperature is reduced to 50 ℃, adding raw materials of 0.1g of salvia miltiorrhiza extract, 0.1g of honeysuckle extract and 0.1g of trichosanthes kirilowii maxim extract and stirring uniformly;
(3) after the temperature is reduced to 40 ℃, 0.4g of polysorbate-20, 0.25g of phenoxyethanol, 0.2g of methylparaben, 0.01g of sodium hydroxide and 0.05g of daily essence are added and stirred uniformly to obtain the salvia miltiorrhiza whitening and freckle removing liquid.
(4) Sequentially filling the whitening and freckle-removing salvia miltiorrhiza liquid into bags with facial mask cloth, packaging, and warehousing after the finished products are qualified.
The mask prepared in example 4 was tested for hygienic chemical and microbiological indicators and the results are shown in tables 14 and 15.
TABLE 14
| Inspection item | Index (I) | Example 3 |
| Mercury | ≤1mg/kg | 0.11 |
| Lead (in lead) | ≤10mg/kg | 1.2 |
| Arsenic (measured as arsenic) | ≤2mg/kg | 0.32 |
| Hydroquinone | Cannot be detected | Not detected out |
| Phenol and its preparation | Cannot be detected | Not detected out |
| pH value | 4.0-8.5 | 6.8 |
| Cadmium (Cd) | ≤5mg/kg | 1.25 |
Watch 15
As can be seen from tables 14 and 15, the hygienic chemical and microbiological indicators of the facial mask prepared according to the present invention meet the requirements of the relevant standards.
Test example 1
The invention researches the oxidation resistance by taking the tyrosinase inhibition rate as an index, and researches the oxidation resistance of the natural facial mask which takes the salvia miltiorrhiza, the honeysuckle and the trichosanthes kirilowii maxim as raw materials to observe the synergistic effect among the active ingredients of different traditional Chinese medicines.
Sample preparation: the salvia miltiorrhiza extract prepared in example 1 was set as group a; the honeysuckle flower extract prepared in example 2 was set as group B; the trichosanthes kirilowii maxim extract prepared in example 3 was set as group C; mixing the Saviae Miltiorrhizae radix extract of example 1 and the fructus Trichosanthis extract prepared in example 3, and making into group D; mixing the flos Lonicerae extract prepared in example 2 and the fructus Trichosanthis extract prepared in example 3, and setting as group E; mixing the prepared salvia miltiorrhiza extract prepared in example 1 and the honeysuckle flower extract prepared in example 2 to prepare a group F; mixing the salvia miltiorrhiza extract prepared in example 1, the honeysuckle flower extract prepared in example 2 and the trichosanthes kirilowii extract prepared in example 3 to prepare a group G;
the mass fraction of each extract in each group of samples was the same. And mixing each group of samples with water to prepare mask liquid with the total mass fraction of 0.3%.
Determination of whitening Performance of Natural facial mask solution
The mushroom is cleaned and cut into small pieces, 20g of the small pieces are weighed and placed in a 100mL beaker, and 100mL of phosphate buffer solution with the pH value of 6.8 and the concentration of 0.3mol/L is added. After stirring, 3 layers of gauze are used for filtering treatment, filtered liquid is selected for the test, and the filtrate is centrifuged at 4000rpm for 15min, and then supernatant is taken for later use. 28 10mL centrifuge tubes were prepared and divided into 7 groups, and each group of centrifuge tubes was added with a group of 1.2mL facial mask solution, 0.2mL enzyme solution (supernatant), distilled water, 2mL PBS buffer, and 1mL catechol solution, in the amounts shown in Table 16. The tubes were left at 25 ℃ for 10min, 1.5mL of 0.025mol/L catechol solution were added, the tubes were left at 25 ℃ for 10min, and the absorbance was measured at a maximum absorption wavelength λ max of 458. And (3) recording the absorbance value with the centrifugal tube number a as Aa, recording the absorbance value with the centrifugal tube number b as Ab, recording the absorbance value with the centrifugal tube number c as Ac, recording the absorbance value with the centrifugal tube number d as Ad, and then calculating the tyrosinase inhibition rate I according to a formula. The inhibition rate was [ (Aa-Ab) - (Ac-Ad) ]/(Aa-Ab) × 100%. The results are shown in FIG. 1.
TABLE 16
As can be seen from FIG. 1, by examining the inhibition rate of facial mask solutions of 7 different formulations on tyrosinase, the inhibition effect is as follows: 21.1%, 16.7%, 22.5%, 65.7%, 71.1%, 78.2%, 87.8%, i.e. G > F > E > D > C > A > B. The inhibition rate of tyrosinase activity is detected to judge that the higher the inhibition rate, the less melanin is produced by melanocytes, and the better the whitening effect is. The results show that the 7 natural facial mask liquids have certain whitening effects, wherein the inhibition effect of G on the activity of tyrosinase is the highest and is 87.8%.
Test example 2: animal experiment verification mask safety
In order to ensure the use safety of the mask, the invention carries out skin irritation and allergy experiments on the mask, and provides basis for safe clinical use.
1. Skin irritation test
After 7 days of acclimatization, the rabbits were depilated on both sides of their backs with a shearing knife and an electric hair clipper. The epilation area per side is about 50cm 2. After 24 hours, the depilated skin was examined. No damage due to depilation, skin irritation experiments can be performed. Rabbits were divided into intact skin group and damaged skin group, 6 per group, half male. The injured skin group was wiped with a sandpaper, the skin on both sides of the back was treated to a degree that the skin was slightly bleeding, the intact skin group was not treated, and then 0.5ml of facial mask solution was applied to the skin on both sides of the back of the mouse. Observing with naked eyes under natural light for 1, 24, 48 and 72h after applying facial mask liquid, observing with naked eyes under natural light, recording daily irritation such as erythema and edema of rabbit according to Table 1, and evaluating skin irritation intensity according to tables 17 and 18.
TABLE 17
Watch 18
2. Skin allergy test
After 3 days of acclimatization, SD rats were subjected to bilateral spinal depilation with a depilation area of about 3cm by 3cm per side. The rat skin was observed after 24 hours and the skin was not damaged by depilation. Skin allergy test can be performed. The rats are divided into a blank group, a positive control group and a facial mask group 3 by adopting a random digital table method, wherein each group comprises 8 rats, and the male rat and the female rat account for half of the rats respectively. Contact sensitization; the rats were taken on the left dorsal depilated area, the blank group was treated with about 0.5ml of physiological saline, the positive control group was treated with about 0.5ml of 2,4-2 nitrochlorobenzene (acetone concentration 1%), and the mask group of preparation example 1 was treated with about 0.5ml of liquid paint. After 6h, the drug was immediately rinsed and observed, and skin allergic reactions were again observed at 24h, 48h, and 72 h. And (3) exciting contact: the right dorsal depilated area of the rat was taken 7 days after the last sensitization of the test substance. The rats in the blank group were externally coated with about 0.5ml of physiological saline, the positive control group was about 0.5ml of 2, 4-dinitrochlorobenzene (0.1% acetone for stimulation), and the mask group was externally coated with about 0.5ml of mask solution, and after 6 hours, the drugs were washed, immediately observed, and the skin allergy was observed again at 24, 48, and 72 hours. The allergic response scores at each time point were recorded as in table 3 and the mean response score for each group of rats was calculated. Mean response score ═ total score for erythema formation + total score for edema formation)/total number of animals. Sensitization was inferred from the incidence of sensitization (incidence of sensitization ═ number of animals that developed anaphylaxis ÷ total number of animals x 100%). Meanwhile, the severe reactions of asthma, unstable standing, shock and the like are observed on the experimental animals, and the scoring standards are shown in tables 19 and 20.
Watch 19
3 results
(1) Skin irritation test results of facial mask
In the experimental process, the appearance, behavior, ingestion and excretion of each group of rabbits are normal, the body weight fluctuation is normal, and no abnormal change exists. Has no abnormal pruritus, pigmentation, bleeding spots, rough skin, thin skin and the like. All animals were in good condition and did not die from the start of the experiment to the end of the observation. No erythema edema was seen on intact skin and damaged skin at different time points 1, 24, 48, 72 hours after the peeling of the membrane. The skin irritation scores in both groups were 0, indicating that the facial mask fluid was not irritating to the skin. This is shown in table 21.
TABLE 21
(2) Results of allergy test
During the experiment, rats in each group had good drinking, eating, defecation and mental status, and no abnormal change in body weight. No erythema or edema appears on the skin of the blank group and the facial mask group in the depilated area of the rat 6-72 hours after the excitation and the drug administration, and no sensitization reaction exists.
The positive control group rats have erythema and edema formation of different degrees on the skin after the excitation administration, the sensitization reaction incidence rate reaches 75 percent, and the skin still has erythema and edema after 72 hours. Suggesting that the facial mask liquid is not sensitive to the skin. See table 22.
TABLE 22
The above description is only a preferred embodiment of the present application and is not intended to limit the present application, and various modifications and changes may be made by those skilled in the art. Any modification, equivalent replacement, improvement and the like made within the spirit and principle of the present application shall be included in the protection scope of the present application.
Claims (10)
1. A whitening and freckle-removing composition of red sage roots is characterized by comprising a red sage root extract, a honeysuckle flower extract and a trichosanthes kirilowii maxim extract; the mass ratio of the salvia miltiorrhiza extract to the honeysuckle flower extract to the trichosanthes kirilowii maxim extract is 1:1: 1.
2. The red sage root whitening and freckle removing composition according to claim 1, wherein the red sage root extract is prepared by the following method: mixing the salvia miltiorrhiza with deionized water, soaking, decocting and extracting for 2 times, combining filtrates, concentrating under reduced pressure until the relative density is 1.18-1.22, and vacuum drying to obtain the salvia miltiorrhiza extract.
3. The red sage root whitening and freckle removing composition according to claim 2, wherein the mass ratio of the red sage root to the deionized water is 1: 12; decocting and extracting for 2 hours each time; the temperature of the reduced pressure concentration was 60 ℃.
4. The red sage root whitening and freckle removing composition according to claim 1, wherein the honeysuckle flower extract is prepared by the following method: mixing flos Lonicerae with 8 mass times of deionized water, extracting at 90 deg.C for 1 hr, filtering, adding 6 mass times of deionized water into the residue, extracting at 90 deg.C for 1 hr, filtering, mixing filtrates, centrifuging, filtering to obtain flos Lonicerae extract, concentrating, and freeze drying to obtain flos Lonicerae extract.
5. The red sage root whitening and freckle removing composition of claim 1, wherein the trichosanthes kirilowii maxim extract is prepared by the following method: mixing fructus Trichosanthis with 40% ethanol water solution, extracting at 60 deg.C for 1 hr, extracting for 2 times, mixing extractive solutions, recovering ethanol, centrifuging, filtering to obtain fructus Trichosanthis extractive solution, concentrating, and vacuum drying under reduced pressure to obtain fructus Trichosanthis extract.
6. The red sage root whitening and freckle removing composition of claim 5, wherein the mass ratio of the trichosanthes kirilowii Maxim to the ethanol aqueous solution is 1: 8.
7. The use of the red sage root whitening and freckle removing composition according to any one of claims 1 to 6 in the preparation of a red sage root whitening and freckle removing product.
8. A whitening and freckle-removing mask containing Salvia miltiorrhiza Bunge as claimed in any one of claims 1 to 6 as an active ingredient.
9. The radix salviae miltiorrhizae whitening and freckle removing mask according to claim 8 is characterized by comprising the following raw materials in percentage by weight:
6-8% of butanediol, 3-5% of glycerol, 3-5% of dipropylene glycol, 0.5% of diglycerol, 0.5% of sucrose, 200.4% of polysorbate, 0.25% of phenoxyethanol, 320.2% of polyethylene glycol, 0.2% of methyl hydroxybenzoate, 0.086% of carbomer, 0.01% of sodium hydroxide, 0.05% of daily essence, 0.1% of salvia miltiorrhiza extract, 0.1% of honeysuckle flower extract, 0.1% of trichosanthes kirilowii maxim extract and the balance of water.
10. The preparation method of the salvia miltiorrhiza mask for whitening and removing freckles as claimed in claim 8 or 9, which is characterized by comprising the following steps:
(1) mixing water, butanediol, glycerol, dipropylene glycol, diglycerol, sucrose, polyethylene glycol-32 and carbomer, heating to 80-85 ℃, homogenizing and stirring uniformly;
(2) adding Saviae Miltiorrhizae radix extract, flos Lonicerae extract and fructus Trichosanthis extract when the temperature is reduced to 50 deg.C, and stirring;
(3) after the temperature is reduced to 40 ℃, adding polysorbate-20, phenoxyethanol, methylparaben, sodium hydroxide and daily essence, and uniformly stirring to obtain the salvia miltiorrhiza whitening and freckle removing liquid;
(4) and (4) filling the red sage root whitening and freckle removing liquid obtained in the step (3) into a bag filled with a mask cloth to obtain the red sage root whitening and freckle removing mask.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011310970.3A CN112245363B (en) | 2020-11-20 | 2020-11-20 | Salvia miltiorrhiza whitening and freckle removing mask |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011310970.3A CN112245363B (en) | 2020-11-20 | 2020-11-20 | Salvia miltiorrhiza whitening and freckle removing mask |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN112245363A true CN112245363A (en) | 2021-01-22 |
| CN112245363B CN112245363B (en) | 2022-02-11 |
Family
ID=74224947
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202011310970.3A Active CN112245363B (en) | 2020-11-20 | 2020-11-20 | Salvia miltiorrhiza whitening and freckle removing mask |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN112245363B (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110585084A (en) * | 2019-09-27 | 2019-12-20 | 罗梅英 | Blend liquid of Chinese herbal medicine mask powder and preparation method and application thereof |
| CN113197800A (en) * | 2021-04-21 | 2021-08-03 | 宁波久瑞生物工程有限公司 | Anti-allergy composition, anti-allergy essence and preparation method thereof |
| CN113786365A (en) * | 2021-09-29 | 2021-12-14 | 怀化学院 | Preparation method of traditional Chinese medicine freckle-removing and beautifying face cream |
| CN115581626A (en) * | 2022-08-12 | 2023-01-10 | 植物医生(广东)生物科技有限公司 | Moisturizing, antioxidant and soothing composition and application thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108371643A (en) * | 2018-03-28 | 2018-08-07 | 广州科玛大健康产业有限公司 | A kind of reparation skin rejuvenating facial mask and preparation method thereof |
| CN109364207A (en) * | 2018-11-23 | 2019-02-22 | 上海松皓生物科技有限公司 | A kind of composition and preparation method and the application for the treatment of and alleviation acne |
-
2020
- 2020-11-20 CN CN202011310970.3A patent/CN112245363B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108371643A (en) * | 2018-03-28 | 2018-08-07 | 广州科玛大健康产业有限公司 | A kind of reparation skin rejuvenating facial mask and preparation method thereof |
| CN109364207A (en) * | 2018-11-23 | 2019-02-22 | 上海松皓生物科技有限公司 | A kind of composition and preparation method and the application for the treatment of and alleviation acne |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110585084A (en) * | 2019-09-27 | 2019-12-20 | 罗梅英 | Blend liquid of Chinese herbal medicine mask powder and preparation method and application thereof |
| CN113197800A (en) * | 2021-04-21 | 2021-08-03 | 宁波久瑞生物工程有限公司 | Anti-allergy composition, anti-allergy essence and preparation method thereof |
| CN113786365A (en) * | 2021-09-29 | 2021-12-14 | 怀化学院 | Preparation method of traditional Chinese medicine freckle-removing and beautifying face cream |
| CN115581626A (en) * | 2022-08-12 | 2023-01-10 | 植物医生(广东)生物科技有限公司 | Moisturizing, antioxidant and soothing composition and application thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN112245363B (en) | 2022-02-11 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN112245363B (en) | Salvia miltiorrhiza whitening and freckle removing mask | |
| KR100771829B1 (en) | A composition comprising plant extracts for protecting skin from stress due to harmful environment | |
| CN103816087A (en) | Whitening, sun-resistant and spot removing cosmetic of traditional Chinese medicine composition, and preparation method thereof | |
| KR101387308B1 (en) | Skin whitening composition by using of dendropanax morbifera ferment extract | |
| KR101829892B1 (en) | A cosmetic composition comprising fraxinus rhynchophylla extract and calystegia soldanella extract | |
| CN105726391B (en) | A kind of dendrobium candidum shower cream | |
| US20130101689A1 (en) | Composition containing paper mulberry extracts | |
| KR100564108B1 (en) | Cosmetic composition comprising plant extract having anti-aging effect | |
| WO2007004771A1 (en) | Cosmetic composition containing as available ingredient the extracts of equisetum arvense l. | |
| KR20200124986A (en) | Cosmetic composition comprising bamboo sprout peel extract | |
| CN108852951A (en) | A kind of moistening and cleaning skin composition and plant body wash | |
| KR101046438B1 (en) | Cosmetic composition containing a mixed plant extract having a skin wrinkle improvement and skin astringent action and a method of manufacturing the same | |
| US10434057B2 (en) | Cosmetic composition comprising osmanthus asiaticus extract and clematis apiifolia extract | |
| KR101047612B1 (en) | Cosmetic composition for reducing or treating acne and acne rash | |
| CN112587455A (en) | Plant composition and herbal whitening and freckle removing sleep mask gel | |
| KR101056756B1 (en) | Cosmetic composition containing herb medicine extracts | |
| KR100964428B1 (en) | Cosmetic composition containing natural medicinal plant extract for alleviating skin inflammation | |
| WO2014094380A1 (en) | Bee venom composition with sun-proof effect | |
| KR101252560B1 (en) | Cosmetic composition containing extracts of Impatiens balsamina L. for controlling anti-acne or anti-comedone | |
| KR20170137412A (en) | Composition for improving skin condition comprising herb extracts mixture | |
| CN109568230A (en) | A kind of Chinese medicine cosmetic of the anti-aging of moisturizing whitening | |
| JP2001233727A (en) | Cosmetic | |
| CN112933018B (en) | Composition with barrier repairing and anti-photoaging effects and preparation method and application thereof | |
| KR950005919B1 (en) | Soap compositions | |
| CN103505398B (en) | Forsythia leaf facial mask |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |