CN1122180C - 用于检测乳腺炎的试剂 - Google Patents
用于检测乳腺炎的试剂 Download PDFInfo
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- CN1122180C CN1122180C CN 00120943 CN00120943A CN1122180C CN 1122180 C CN1122180 C CN 1122180C CN 00120943 CN00120943 CN 00120943 CN 00120943 A CN00120943 A CN 00120943A CN 1122180 C CN1122180 C CN 1122180C
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- mastitis
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- mammitis
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- 208000004396 mastitis Diseases 0.000 title claims abstract description 22
- 239000003153 chemical reaction reagent Substances 0.000 title claims abstract description 17
- GLNADSQYFUSGOU-GPTZEZBUSA-J Trypan blue Chemical compound [Na+].[Na+].[Na+].[Na+].C1=C(S([O-])(=O)=O)C=C2C=C(S([O-])(=O)=O)C(/N=N/C3=CC=C(C=C3C)C=3C=C(C(=CC=3)\N=N\C=3C(=CC4=CC(=CC(N)=C4C=3O)S([O-])(=O)=O)S([O-])(=O)=O)C)=C(O)C2=C1N GLNADSQYFUSGOU-GPTZEZBUSA-J 0.000 claims abstract description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 5
- 239000012153 distilled water Substances 0.000 claims abstract description 4
- 241000233855 Orchidaceae Species 0.000 claims description 4
- 229910052708 sodium Inorganic materials 0.000 claims description 2
- -1 NaN2PO4 Chemical compound 0.000 abstract 1
- BNIILDVGGAEEIG-UHFFFAOYSA-L disodium hydrogen phosphate Chemical compound [Na+].[Na+].OP([O-])([O-])=O BNIILDVGGAEEIG-UHFFFAOYSA-L 0.000 abstract 1
- 229910000397 disodium phosphate Inorganic materials 0.000 abstract 1
- 235000019800 disodium phosphate Nutrition 0.000 abstract 1
- 235000013336 milk Nutrition 0.000 description 17
- 239000008267 milk Substances 0.000 description 17
- 210000004080 milk Anatomy 0.000 description 17
- 241000283690 Bos taurus Species 0.000 description 6
- 210000005075 mammary gland Anatomy 0.000 description 6
- 210000004027 cell Anatomy 0.000 description 4
- 238000001514 detection method Methods 0.000 description 4
- 241001465754 Metazoa Species 0.000 description 3
- 201000010099 disease Diseases 0.000 description 3
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 230000000694 effects Effects 0.000 description 2
- 208000015181 infectious disease Diseases 0.000 description 2
- 239000011734 sodium Substances 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 208000035473 Communicable disease Diseases 0.000 description 1
- 206010014824 Endotoxic shock Diseases 0.000 description 1
- 206010061218 Inflammation Diseases 0.000 description 1
- RCEAADKTGXTDOA-UHFFFAOYSA-N OS(O)(=O)=O.CCCCCCCCCCCC[Na] Chemical compound OS(O)(=O)=O.CCCCCCCCCCCC[Na] RCEAADKTGXTDOA-UHFFFAOYSA-N 0.000 description 1
- 241001494479 Pecora Species 0.000 description 1
- 206010040047 Sepsis Diseases 0.000 description 1
- 206010040070 Septic Shock Diseases 0.000 description 1
- 208000019802 Sexually transmitted disease Diseases 0.000 description 1
- QAOWNCQODCNURD-UHFFFAOYSA-L Sulfate Chemical compound [O-]S([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 description 1
- 230000001154 acute effect Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000009833 condensation Methods 0.000 description 1
- 230000005494 condensation Effects 0.000 description 1
- 238000003977 dairy farming Methods 0.000 description 1
- 235000013365 dairy product Nutrition 0.000 description 1
- 239000003599 detergent Substances 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 235000014113 dietary fatty acids Nutrition 0.000 description 1
- 229930195729 fatty acid Natural products 0.000 description 1
- 239000000194 fatty acid Substances 0.000 description 1
- 150000004665 fatty acids Chemical class 0.000 description 1
- 230000028993 immune response Effects 0.000 description 1
- 230000004054 inflammatory process Effects 0.000 description 1
- 210000000265 leukocyte Anatomy 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
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- Investigating Or Analysing Biological Materials (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
本发明提供了一种用于检测乳腺炎的试剂，它由C12H25NaSO4、Na2HPO4、NaH2PO4、锥虫兰(Trypan blue)和蒸馏水组成。该试剂能够快速、简便、准确的用于检测乳牛的乳腺炎，特别是用于检测亚临床乳腺炎。
Description
技术领域
本发明涉及一种用于检测乳腺炎的试剂，尤指一种用于检测奶牛亚临床乳腺炎的试剂。
背景技术
革兰氏阴性菌会引起许多家畜的疾病，包括可能威胁生命的革兰氏阴性败血症和内毒素性休克。每年畜牧业都会因为此类革兰氏阴性传染病造成严重的死亡和经济损失。由于此类革兰氏阴性感染造成的乳腺或乳房的炎症即乳腺炎是传染性很强的，所以发生在乳牛、猪及羊上的此类疾病是传染性极强的疾病。
乳腺炎是乳腺的炎症，是乳牛乳腺为反击外来的侵害，进而修复组织并且恢复乳腺的正常产奶功能造成的。它是造成世界范围的乳牛业经济损失的首要因素。有多种形式的乳腺炎，包括极急性的、急性的、临床的及亚临床的。前面三种是肉眼可察的。然而亚临床的是不太明显的，因而使用现场的检测方法是非常重要的。
正如众所周知的，对于乳腺炎的早期的诊断和治疗对于保证动物的健康及最佳的牛奶质量是很重要的。有多种对于亚临床乳腺炎的检测方法，可使用牛奶样品，在现场或实验室进行检测，但缺少一种能够快速、准确地用来检测乳腺炎的试剂。
发明内容
本发明的目的在于提供一种用于检测乳腺炎的试剂，它能够快速、简便、准确的用于检测乳牛的乳腺炎，特别是用于检测亚临床乳腺炎。
上述目的是通过如下技术方案实现的：一种用于检测乳腺炎的试剂，其成份及含量如下：
C12H25NaSO4 12.1％～12.4％
Na2HPO4 4.69％～4.79％
NaH2PO4 0.906％～0.924％
锥虫兰(Trypan blue) 0.404％～0.413％
蒸馏水 余量
其中各组分的含量为质量百分比。
采用上述试剂的检测方法是基于奶牛的免疫反应会将白血球、体细胞(SCC)及血清蛋白带入牛奶中来。这种检测方法可针对下述三种情况下取得的牛奶使用：
1)在现场直接从乳牛的乳腺取得的牛奶；
2)在实验室中对于从单独的乳腺取得的牛奶；
3)对于从农场的大储奶罐中取得的复合的牛奶样品。
该试验使用阴离子洗涤剂来与乳腺炎牛奶反应来造成一种可观察到的效果，以实现一种参考体细胞记数的一种数字评分办法。检测的原理是牛奶中的传染病反击细胞随着DNA的释放而被试剂破坏，并且形成一种可见的DNA凝结效果。颜色指示剂在由于乳腺炎造成牛奶的pH值升高时变成深紫色。
该测试方法包括下列步骤：
1.将牛奶喷入一圆形器皿(约2毫升)；
2.加入与牛奶样品同等体积的试剂，即试剂与牛奶的体积比至少是1∶1；
3.在水平面上对上述混合物进行轻微的圆圈状搅动；
该反应迅速进行。当牛奶含有高浓度的体细胞时，在10秒钟内达到反应的峰值。若溶液中的凝结非常明显，则被视为阳性。
具体实施方式
实施例1
按照下述各组分的质量百分比混合即合配制出本发明一种优选的试剂：
12.25％的C12H25NaSO4、4.74％的Na2HPO4、0.91％的NaH2PO4、0.41％的锥虫兰(Trypan blue)和81.69％的蒸馏水。
其中C12H25NaSO4为脂肪酸硫酸盐(十二烷基硫酸钠)。
应当指出：因该配方是pH值敏感剂，故在保持其pH值不变的前提下，对其各组分的含量可进行适当的调整。
Claims (2)
1、一种用于检测乳腺炎的试剂，其特征在于它的组份及含量如下：
C12H25NaSO4 12.1％～12.4％
Na2HPO4 4.69％～4.79％
NaH2PO4 0.906％～0.924％
锥虫兰(Trypan blue) 0.404％～0.413％
蒸馏水 余量
其中各组分的含量为质量百分比。
2、如权利要求1所述的用于检测乳腺炎的试剂，其特征在于：它由12.25％的C12H25NaSO4、4.74％的Na2HPO4、0.91％的NaH2PO4、0.41％的锥虫兰(Trypan blue)和81.69％的蒸馏水组成。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 00120943 CN1122180C (zh) | 2000-08-07 | 2000-08-07 | 用于检测乳腺炎的试剂 |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 00120943 CN1122180C (zh) | 2000-08-07 | 2000-08-07 | 用于检测乳腺炎的试剂 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1337579A CN1337579A (zh) | 2002-02-27 |
| CN1122180C true CN1122180C (zh) | 2003-09-24 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN 00120943 Expired - Fee Related CN1122180C (zh) | 2000-08-07 | 2000-08-07 | 用于检测乳腺炎的试剂 |
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Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100460866C (zh) * | 2005-11-23 | 2009-02-11 | 山东奥克斯生物技术有限公司 | 牛、羊、猪乳房炎快速诊断试剂及其制备方法 |
| CN101825626A (zh) * | 2010-04-22 | 2010-09-08 | 中国农业科学院兰州畜牧与兽药研究所 | 一种检测奶牛隐性乳房炎的诊断试剂及制备方法 |
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| Publication number | Publication date |
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| CN1337579A (zh) | 2002-02-27 |
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