CN112189601A - Method suitable for constructing buried-dwelling shellfish families - Google Patents
Method suitable for constructing buried-dwelling shellfish families Download PDFInfo
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- CN112189601A CN112189601A CN202011164984.9A CN202011164984A CN112189601A CN 112189601 A CN112189601 A CN 112189601A CN 202011164984 A CN202011164984 A CN 202011164984A CN 112189601 A CN112189601 A CN 112189601A
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- Prior art keywords
- container
- sperms
- family
- shellfish
- parent
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- 235000015170 shellfish Nutrition 0.000 title claims abstract description 28
- 238000000034 method Methods 0.000 title claims abstract description 19
- 241000237503 Pectinidae Species 0.000 claims abstract description 16
- 235000020637 scallop Nutrition 0.000 claims abstract description 16
- 239000013535 sea water Substances 0.000 claims abstract description 9
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 9
- 238000001035 drying Methods 0.000 claims abstract description 6
- 210000002149 gonad Anatomy 0.000 claims abstract description 6
- 230000003287 optical effect Effects 0.000 claims abstract description 5
- 230000000638 stimulation Effects 0.000 claims abstract description 5
- 238000003756 stirring Methods 0.000 claims abstract description 5
- 238000007599 discharging Methods 0.000 claims abstract description 4
- 235000013601 eggs Nutrition 0.000 claims description 19
- 239000004575 stone Substances 0.000 claims description 7
- 210000004681 ovum Anatomy 0.000 claims description 6
- 102000002322 Egg Proteins Human genes 0.000 claims description 5
- 108010000912 Egg Proteins Proteins 0.000 claims description 5
- 238000004891 communication Methods 0.000 claims description 3
- 238000007689 inspection Methods 0.000 claims description 3
- 238000005273 aeration Methods 0.000 claims description 2
- 206010011409 Cross infection Diseases 0.000 abstract description 3
- 206010029803 Nosocomial infection Diseases 0.000 abstract description 3
- 238000010276 construction Methods 0.000 abstract description 2
- 230000000694 effects Effects 0.000 abstract description 2
- 230000004936 stimulating effect Effects 0.000 description 4
- 238000009395 breeding Methods 0.000 description 3
- 230000001488 breeding effect Effects 0.000 description 3
- 230000000384 rearing effect Effects 0.000 description 3
- 241000237858 Gastropoda Species 0.000 description 2
- 241001339782 Scapharca broughtonii Species 0.000 description 2
- 230000004720 fertilization Effects 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 239000004576 sand Substances 0.000 description 2
- 210000000582 semen Anatomy 0.000 description 2
- 241000593558 Bullacta Species 0.000 description 1
- 235000017003 Cissus Nutrition 0.000 description 1
- 244000035145 Cissus repens Species 0.000 description 1
- 235000017014 Cissus repens Nutrition 0.000 description 1
- 241000585116 Galba truncatula Species 0.000 description 1
- 241000514719 Mactra quadrangularis Species 0.000 description 1
- 241000392375 Sinonovacula constricta Species 0.000 description 1
- 241000894431 Turbinidae Species 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000013505 freshwater Substances 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 230000005070 ripening Effects 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/50—Culture of aquatic animals of shellfish
- A01K61/54—Culture of aquatic animals of shellfish of bivalves, e.g. oysters or mussels
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
The invention discloses a method suitable for constructing a buried shellfish family, which comprises the following steps: taking a plurality of parent scallops with mature gonads, uniformly drying in the shade for stimulation, respectively and independently placing the parent scallops in a plurality of containers containing seawater, and inflating each container; stopping gas supply for discharging, recording and marking the container when sperm and egg discharge occurs in the container, wherein the marking content is male and female and discharge time, and observing the sperm and egg quality under an optical microscope; taking qualified sperms from a container filled with sperms, slowly pouring the sperms into the container filled with ova, adding water, stirring uniformly to enable the sperms and the ova to be in full contact, and performing aerated culture to construct a family; compared with the prior art, the advantages are: after the parent shellfish is uniformly stimulated, the parent shellfish is respectively placed in different containers, so that cross infection is completely avoided; the influence of artificial activities on gamete emission of the parent scallops is reduced, and the parent scallops are utilized to the maximum; the family construction efficiency can be improved.
Description
Technical Field
The invention relates to the technical field of breeding of bivalve shellfish in aquatics, in particular to a method suitable for constructing a buried shellfish family.
Background
The buried shellfish refers to sand mud or sand-sand bivalve and gastropod which crawl or bury in the intertidal zone, the low tide zone and the sub-tidal zone within 20 m. The cultivation of buried shellfish has a long history in China. The vast majority of buried shellfish cultured in China are bivalve shells, and the majority is as follows: blood clam, razor clam, giant razor clam, long razor clam, variegated clam, etc. There are only a few gastropods, such as the bullacta, the red shells, the turban shells and the like. In recent years, the cultivation of buried shellfish is rapidly developed, the cultivation area is continuously enlarged, the cultivation variety is gradually increased, and the cultivation yield is also improved year by year.
In recent years, some kinds of indoor artificial breeding such as blood clam, mactra veneriformis, clam, sinonovacula constricta, cissus tongue, mud snail and the like have been developed and tried successively in the culture and production of buried shellfish in China. The indoor artificial breeding of shellfish is a production process with very strong technology, and comprises the steps of parent shellfish selection, temporary rearing, gonad ripening and spawning promotion, artificial fertilization, egg washing, larva rearing and the like, and especially the rearing of larvae is most important.
Most of the existing methods for constructing the family of the buried shellfish are to grab the individuals who are spermiation and spawning, wash the individuals with fresh water, place the individuals into a container filled with seawater to singly spawn and spermiate, and respectively collect sperms and ova. After the parent shellfish discharges the sperms and the eggs respectively, the parent shellfish is removed, the semen is diluted, the semen is poured into a pool containing the eggs according to a certain amount, and the mixture is stirred uniformly, so that the artificial fertilization is realized. However, the method for uniformly stimulating the uniform spermiation and spawning can affect the discharge of shellfish gametes to a certain extent, possibly affect the quality and quantity of the gametes, and can avoid cross infection among different parent shellfish gametes only by strict operation.
Disclosure of Invention
In order to solve the technical problems, the invention aims to provide a method suitable for constructing buried-dwelling type shellfish families, aiming at improving the efficiency and accuracy of constructing the buried-dwelling type shellfish families.
Therefore, the invention provides a method suitable for constructing a buried shellfish family, which comprises the following steps:
s01, taking a plurality of parent scallops with mature gonads, uniformly drying in the shade for stimulation, respectively and independently placing the parent scallops in a plurality of containers containing seawater, and inflating each container;
s02, stopping gas supply for discharging, recording and marking the container when sperm and egg discharge occurs in the container, wherein the marking content is male and female and the discharge time, and observing the sperm and egg quality under an optical microscope;
s03, taking the qualified sperms from the container filled with the sperms, slowly pouring the sperms into the container filled with the eggs, adding water, stirring uniformly to enable the sperms and the eggs to be in full contact, and performing aerated culture to construct a family.
Compared with the prior art, the method disclosed by the invention has the following advantages: after the parent scallops are uniformly stimulated, the parent scallops are respectively and independently placed in different containers for culture, and the method is different from the traditional method for uniformly stimulating to uniformly discharge sperms and lay eggs, and the method for uniformly stimulating to discharge sperms and lay eggs independently can completely avoid cross infection, reduce the influence of human activities on gamete discharge of the parent scallops, and maximize the utilization of gametes of the parent scallops; the family construction efficiency can be improved.
In a preferred embodiment of the present invention, in the step S01, the drying stimulation time is 4 to 12 hours.
In another preferred embodiment of the present invention, in the step S01, the aeration time is 1 to 1.5 hours.
In another preferred embodiment of the present invention, in step S01, the container is a beaker with a capacity of 2-4 liters, the beaker is filled with 1-2 liters of seawater and is pre-provided with a gas stone, and the gas stone is in gas flow communication with an inflation source so as to inflate the beaker.
In a further preferred embodiment, in step S03, 0.5 to 1 liter of sperm qualified for quality inspection is taken from a beaker containing sperm, slowly poured into the beaker containing ovum, and water is added to the beaker containing ovum to 2 to 4 liters.
Detailed Description
It is easily understood that, according to the technical solution of the present invention, a plurality of alternative structures and implementations can be proposed by those skilled in the art without changing the spirit of the present invention. Therefore, the following detailed description is merely illustrative of the technical solutions of the present invention, and should not be construed as being all of the present invention or limiting or restricting the technical solutions of the present invention.
A method suitable for constructing buried-dwelling shellfish families comprises the following steps:
s01, taking a plurality of parent scallops with mature gonads, uniformly drying in the shade and stimulating, respectively and independently placing the parent scallops in a plurality of containers for containing seawater, inflating each container, wherein each container is a beaker with the capacity of 2-4 liters, 1-2 liters of seawater is contained in each beaker, air stones are preset in each beaker, an air flow communication is formed between an inflation source such as an air blower or an air pump and the air stones so as to inflate the beakers, and water flow is manufactured, wherein the inflation time is 1-1.5 hours;
s02, stopping gas supply for discharging, recording and marking the container when sperm and egg discharge occurs in the container, wherein the marking content is male and female and the discharge time, and observing the sperm and egg quality under an optical microscope;
s03, taking 0.5-1 liter of qualified sperms from the beaker filled with the sperms, slowly pouring the sperms into the beaker filled with the eggs, adding water to the beaker filled with the sperms to 2-4 liters, uniformly stirring to ensure that the sperms and the eggs are fully contacted, and performing aerated culture to construct a family.
The specific embodiment is realized as follows:
the first step is as follows: a plurality of parent scallops with mature gonads are taken, dried in the shade and stimulated for 4-10 hours, the parent scallops are placed in 2L beakers filled with 1L seawater, each beaker is provided with air stones, an air blower is used for inflating for 1-1.5 hours, and then air is stopped for exhausting.
The second step is that: after discharge was found, the sperm and egg quality was observed under an optical microscope, recorded and marked as male and female and discharge time.
The third step: 0.5L of sperm qualified in quality inspection is taken from the beaker filled with the sperm, slowly poured into the beaker filled with the ovum, and added with water to 2L to construct a family.
The fourth step: stirring with glass rod, making sperm and egg contact thoroughly, and culturing with gas.
By using the method, 30 clam families are successfully established in 2019, 100 clam families are successfully established in 2020, the spawning induction rate is as high as 80%, and the success rate and the efficiency of family establishment are improved while labor is greatly saved.
The technical scope of the present application is not limited to the contents in the above description, and those skilled in the art can make various changes and modifications to the above embodiments without departing from the technical spirit of the present application, and these changes and modifications should fall within the protective scope of the present application.
Claims (5)
1. A method suitable for constructing buried-dwelling shellfish families is characterized by comprising the following steps:
s01, taking a plurality of parent scallops with mature gonads, uniformly drying in the shade for stimulation, respectively and independently placing the parent scallops in a plurality of containers containing seawater, and inflating each container;
s02, stopping gas supply for discharging, recording and marking the container when sperm and egg discharge occurs in the container, wherein the marking content is male and female and the discharge time, and observing the sperm and egg quality under an optical microscope;
s03, taking the qualified sperms from the container filled with the sperms, slowly pouring the sperms into the container filled with the eggs, adding water, stirring uniformly to enable the sperms and the eggs to be in full contact, and performing aerated culture to construct a family.
2. The method for constructing the family of buried shellfish according to claim 1, wherein: in the step S01, the drying stimulation time is 4 to 10 hours.
3. The method for constructing the family of buried shellfish according to claim 1, wherein: in the step S01, the aeration time is 1 to 1.5 hours.
4. The method for constructing the family of buried shellfish according to claim 1, wherein: in the step S01, the container is a beaker with a capacity of 2-4 liters, 1-2 liters of seawater is contained in the beaker, air stones are preset in the beaker, and the container is in airflow communication with the air stones through an inflation source so as to inflate the beaker and produce water flow.
5. The method for constructing the family of buried shellfish according to claim 4, wherein: in the step S03, 0.5 to 1 liter of sperm qualified for quality inspection is taken from the beaker containing sperm, slowly poured into the beaker containing ovum, and added with water to 2 to 4 liters of the beaker containing ovum.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011164984.9A CN112189601A (en) | 2020-10-27 | 2020-10-27 | Method suitable for constructing buried-dwelling shellfish families |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011164984.9A CN112189601A (en) | 2020-10-27 | 2020-10-27 | Method suitable for constructing buried-dwelling shellfish families |
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| Publication Number | Publication Date |
|---|---|
| CN112189601A true CN112189601A (en) | 2021-01-08 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202011164984.9A Pending CN112189601A (en) | 2020-10-27 | 2020-10-27 | Method suitable for constructing buried-dwelling shellfish families |
Country Status (1)
| Country | Link |
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| CN (1) | CN112189601A (en) |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101347104A (en) * | 2008-09-12 | 2009-01-21 | 大连水产学院 | Method for establishing stemma of common intertidal shellfish |
| CN106069957A (en) * | 2016-07-29 | 2016-11-09 | 青岛农业大学 | The method that the single pairing of a kind of male and female builds Sinonovacula constricta family |
| CN108541636A (en) * | 2018-01-21 | 2018-09-18 | 青岛农业大学 | The breeding method of female Alaska scallop and male Yessoensis new varieties |
-
2020
- 2020-10-27 CN CN202011164984.9A patent/CN112189601A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101347104A (en) * | 2008-09-12 | 2009-01-21 | 大连水产学院 | Method for establishing stemma of common intertidal shellfish |
| CN106069957A (en) * | 2016-07-29 | 2016-11-09 | 青岛农业大学 | The method that the single pairing of a kind of male and female builds Sinonovacula constricta family |
| CN108541636A (en) * | 2018-01-21 | 2018-09-18 | 青岛农业大学 | The breeding method of female Alaska scallop and male Yessoensis new varieties |
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Application publication date: 20210108 |