CN112167267A - Compound biological agent for preventing and treating tomato root rot and preparation method thereof - Google Patents
Compound biological agent for preventing and treating tomato root rot and preparation method thereof Download PDFInfo
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- CN112167267A CN112167267A CN202011215888.2A CN202011215888A CN112167267A CN 112167267 A CN112167267 A CN 112167267A CN 202011215888 A CN202011215888 A CN 202011215888A CN 112167267 A CN112167267 A CN 112167267A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
- A01N63/22—Bacillus
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/08—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing solids as carriers or diluents
- A01N25/10—Macromolecular compounds
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
- A01N63/38—Trichoderma
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Abstract
The invention belongs to the technical field of microbial agricultural application, and particularly relates to a compound biological agent for preventing and treating tomato root rot and a preparation method thereof. The invention uses corn stalk and corncob as the thallus carrier, the capability of attaching microorganism is enhanced, meanwhile, the carrier treating agent provides good growth environment for the survival of microorganism, the survival capability of microorganism is increased, the raw material source is wide and easy to obtain, and the carrier treating agent can be degraded in soil, and the thallus growth provides nutrients; in addition, the tomato root rot is synergistically controlled by the bacillus belgii, the bacillus subtilis, the trichoderma and the celery powder, the preparation process is simple, the cost is low, the tomato root rot is pure natural, and the problems of residues and drug resistance do not exist.
Description
Technical Field
The invention belongs to the technical field of microbial agricultural application, and particularly relates to a compound biological agent for preventing and treating tomato root rot and a preparation method thereof.
Background
The tomato contains vitamins, organic acid and a small amount of protein, is rich in nutrition, not only can be eaten as fruits and vegetables, but also can be used as a processing raw material, and has wide application, so that the tomato is popular with cultivators and consumers. With the continuous expansion of the planting area of tomatoes, the planting structure is single, and the continuous cropping years are prolonged, so that the soil-borne diseases such as tomato root rot and stem base rot are increasingly serious. Although the bactericide is the most effective method for preventing and treating tomato soil-borne diseases, the bactericide has short duration, poor degradability and high possibility of generating drug resistance, and seriously influences the ecology. The biological control is not only environment-friendly, but also pathogenic bacteria are not easy to generate drug resistance, thereby causing wide attention of people and achieving the effect at first sight. At present, a plurality of bacilli with high-efficiency bacteriostatic activity are developed, most of the strains have good disease prevention effect and obvious growth promotion effect, but in practice production, the biocontrol bacteria prevention effect is easily influenced by the environment, the effect is unstable, the cost is high, and the bacillus cannot completely replace chemical agents. Therefore, the technology for preventing and controlling the tomato soil-borne diseases is not limited to biological control of a single strain.
Disclosure of Invention
Aiming at the defects of the prior art, the invention provides the compound biological agent for preventing and treating the tomato root rot and the preparation method thereof, aiming at solving the technical problems that the biocontrol bacterium prevention and treatment effect of a single strain is easily influenced by the environment, the effect is unstable, the cost is higher and chemical agents cannot be completely replaced in the prior art.
The invention provides a preparation method of a compound biological agent for preventing and treating tomato root rot, which has the following specific technical scheme:
the preparation method of the compound biological agent for preventing and treating the tomato root rot comprises the following steps:
s1, crushing the corn straws and the corn straws into fine powder of 80-160 meshes, and then sterilizing to obtain a microbial inoculum carrier;
s2, mixing Bacillus belgii, Bacillus subtilis, Trichoderma, livestock and poultry manure, pond sludge and waste fruit and vegetable residues, and performing composting fermentation to obtain microbial agent fermentation product;
s3, naturally drying celery leaves, celery seeds and celery stems in the air, and crushing by using an ultrafine crusher to obtain celery powder;
s4, mixing the microbial inoculum carrier in the step S1, the microbial inoculum leavening in the step S2 and the celery powder in the step S3, drying and crushing to obtain the compound biological microbial inoculum for preventing and treating the tomato root rot.
In certain embodiments, in step S1, the corn stover and the corn stover are in a weight ratio of 1: (2-5), wherein the sterilization temperature is 115 ℃ and 121 ℃, and the sterilization time is 30-60 min.
In some embodiments, the method is characterized in that in step S2, the bacillus bellisii is 0.1-0.5 parts, the bacillus subtilis is 0.1-0.5 parts, the trichoderma is 0.01-0.05 parts, the livestock manure is 25-50 parts, the pond sludge is 40-60 parts, and the waste fruit and vegetable residues are 10-30 parts by weight.
In certain embodiments, the fermentation temperature is 50-60 ℃ and the fermentation time is 15-20 days in step S2.
In certain embodiments, in step S3, the weight ratio of celery leaves, celery seeds and celery stems is 6: 2: 2.
in certain embodiments, in step S4, the weight ratio of the microbial agent carrier, the microbial agent ferment, and the celery powder is 1: (4-6): (1-2).
In certain embodiments, in step S4, the drying temperature is 30-50 ℃ and the moisture content after drying is 10-15%.
The invention also provides a compound biological agent for preventing and treating tomato root rot, and the compound biological agent prepared by the method.
The invention has the following beneficial effects: the invention uses corn stalk and corncob as the thallus carrier, the capability of attaching microorganism is enhanced, meanwhile, the carrier treating agent provides good growth environment for the survival of microorganism, the survival capability of microorganism is increased, the raw material source is wide and easy to obtain, and the carrier treating agent can be degraded in soil, and the thallus growth provides nutrients; in addition, the tomato root rot is synergistically controlled by the bacillus belgii, the bacillus subtilis, the trichoderma and the celery powder, the preparation process is simple, the cost is low, the tomato root rot is pure natural, and the problems of residues and drug resistance do not exist.
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FIG. 1 is a preparation method of the compound biological agent for preventing and treating tomato root rot provided by the invention.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is described in further detail below with reference to the accompanying drawings 1 in conjunction with specific embodiments.
Example 1
The preparation method of the composite biological agent for preventing and treating tomato root rot provided by the embodiment has the following specific technical scheme:
the preparation method of the compound biological agent for preventing and treating the tomato root rot comprises the following steps:
s1, mixing corn stalks and corn stalks according to a weight ratio of 1: 2, mixing, crushing into 80-mesh fine powder, and sterilizing (the sterilization temperature is 115 ℃ and the sterilization time is 60min) to obtain the microbial inoculum carrier.
S2, mixing 0.1 part of Bacillus belgii, 0.1 part of Bacillus subtilis, 0.0 part of trichoderma, 25 parts of livestock and poultry manure, 40 parts of pond sludge and 10 parts of waste fruit and vegetable residues in parts by weight, and performing composting fermentation at the fermentation temperature of 50-60 ℃ for 15-20 days to obtain the microbial inoculum fermentation product.
S3, mixing celery leaves, celery seeds and celery stems according to the weight ratio of 6: 2: 2, naturally drying and crushing by using an ultrafine crusher to obtain the celery powder.
S4, mixing the microbial inoculum carrier and the microbial inoculum fermentation product, and mixing the celery powder according to the weight ratio of 1: 4: 1, drying at the drying temperature of 30 ℃, and crushing to obtain the tomato root rot disease control compound biological agent, wherein the water content after drying is 15%.
Example 2
The preparation method of the composite biological agent for preventing and treating tomato root rot provided by the embodiment has the following specific technical scheme:
the preparation method of the compound biological agent for preventing and treating the tomato root rot comprises the following steps:
s1, mixing corn stalks and corn stalks according to a weight ratio of 1: 5, mixing, crushing into fine powder of 160 meshes, and sterilizing (the sterilization temperature is 121 ℃ and the sterilization time is 30min) to obtain the microbial inoculum carrier.
S2, mixing 0.5 part of Bacillus belgii, 0.5 part of Bacillus subtilis, 0.05 part of trichoderma, 50 parts of livestock and poultry manure, 60 parts of pond sludge and 30 parts of waste fruit and vegetable residues in parts by weight, and performing composting fermentation at the temperature of 50-60 ℃ for 15-20 days to obtain the microbial inoculum fermentation product.
S3, mixing celery leaves, celery seeds and celery stems according to the weight ratio of 6: 2: 2, naturally drying and crushing by using an ultrafine crusher to obtain the celery powder.
S4, mixing the microbial inoculum carrier and the microbial inoculum fermentation product, and mixing the celery powder according to the weight ratio of 1: 6: 1, drying at 50 ℃, and crushing to obtain the tomato root rot disease control compound biological agent, wherein the water content after drying is 10%.
Example 3
The preparation method of the composite biological agent for preventing and treating tomato root rot provided by the embodiment has the following specific technical scheme:
the preparation method of the compound biological agent for preventing and treating the tomato root rot comprises the following steps:
s1, mixing corn stalks and corn stalks according to a weight ratio of 1: 4, mixing, crushing into 120-mesh fine powder, and sterilizing (the sterilization temperature is 115 ℃ and the sterilization time is 45min) to obtain the microbial inoculum carrier.
S2, mixing 0.3 part of Bacillus belgii, 0.2 part of Bacillus subtilis, 0.03 part of trichoderma, 40 parts of livestock and poultry manure, 50 parts of pond sludge and 20 parts of waste fruit and vegetable residues in parts by weight, and performing composting fermentation at the temperature of 50-60 ℃ for 15-20 days to obtain the microbial inoculum fermentation product.
S3, mixing celery leaves, celery seeds and celery stems according to the weight ratio of 6: 2: 2, naturally drying and crushing by using an ultrafine crusher to obtain the celery powder.
S4, mixing the microbial inoculum carrier and the microbial inoculum fermentation product, and mixing the celery powder according to the weight ratio of 1: 5: 1.5, drying at 40 ℃, and crushing to obtain the tomato root rot disease control compound biological agent, wherein the water content after drying is 12%.
In order to further verify the effect of the compound biological agent for preventing and treating tomato root rot, the following experiment is specially carried out. Four areas are selected, the composite biological agents of the embodiment 1, the embodiment 2 and the embodiment 3 are applied respectively, common farmyard manure is used as a comparative example, other experimental conditions are consistent, the prevalence rate of tomato root rot is guaranteed, and the experimental results are shown in table 1.
TABLE 1 prevalence of tomato root rot
| Example 1 | Example 2 | Example 3 | Comparative example | |
| Prevalence of root rot (%) | 10.76 | 12.31 | 9.83 | 38.74 |
In conclusion, the composite microbial inoculum provided by the invention can effectively reduce the incidence rate of the root rot of tomatoes, the corn straws and the corncobs are used as the microbial carriers, the capability of attaching microorganisms is enhanced, meanwhile, the carrier treating agent provides a good growth environment for the survival of the microorganisms, the survival capability of the microorganisms is increased, the raw material sources are wide and easy to obtain, and the composite microbial inoculum can be degraded in soil and provide nutrients for the growth of the microorganisms; in addition, the tomato root rot is synergistically controlled by the bacillus belgii, the bacillus subtilis, the trichoderma and the celery powder, the preparation process is simple, the cost is low, the tomato root rot is pure natural, and the problems of residues and drug resistance do not exist.
The above description is only for the purpose of illustrating preferred embodiments of the present invention and is not to be construed as limiting the invention, and the present invention is not limited to the above examples, and those skilled in the art should also be able to make various changes, modifications, additions or substitutions within the spirit and scope of the present invention.
Claims (8)
1. The preparation method of the compound biological agent for preventing and treating the tomato root rot is characterized by comprising the following steps of:
s1, crushing the corn straws and the corn straws into fine powder of 80-160 meshes, and then sterilizing to obtain a microbial inoculum carrier;
s2, mixing Bacillus belgii, Bacillus subtilis, Trichoderma, livestock and poultry manure, pond sludge and waste fruit and vegetable residues, and performing composting fermentation to obtain microbial agent fermentation product;
s3, naturally drying celery leaves, celery seeds and celery stems in the air, and crushing by using an ultrafine crusher to obtain celery powder;
s4, mixing the microbial inoculum carrier in the step S1, the microbial inoculum leavening in the step S2 and the celery powder in the step S3, drying and crushing to obtain the compound biological microbial inoculum for preventing and treating the tomato root rot.
2. The preparation method of the compound biological agent for preventing and treating tomato root rot according to claim 1, wherein in step S1, the weight ratio of the corn stalks to the corn stalks is 1: (2-5), wherein the sterilization temperature is 115 ℃ and 121 ℃, and the sterilization time is 30-60 min.
3. The preparation method of the compound biological agent for controlling tomato root rot according to claim 1, characterized in that in step S2, the bacillus beilesensis is 0.1-0.5 parts, the bacillus subtilis is 0.1-0.5 parts, the trichoderma is 0.01-0.05 parts, the livestock manure is 25-50 parts, the pond sludge is 40-60 parts, and the waste fruit and vegetable residues are 10-30 parts by weight.
4. The method for preparing a compound biological agent for controlling tomato root rot according to claim 1, wherein in step S2, the fermentation temperature is 50-60 ℃ and the fermentation time is 15-20 days.
5. The method for preparing the composite biological agent for controlling the tomato root rot according to claim 1, wherein in the step S3, the weight ratio of celery leaves, celery seeds and celery stems is 6: 2: 2.
6. the preparation method of the compound biological agent for preventing and treating tomato root rot according to claim 1, wherein in step S4, the weight ratio of the agent carrier, the agent fermentation product and the celery powder is 1: (4-6): (1-2).
7. The preparation method of the compound biological agent for controlling tomato root rot according to claim 1, wherein in step S4, the drying temperature is 30-50 ℃, and the water content after drying is 10-15%.
8. The compound biological agent for preventing and treating tomato root rot is characterized by being prepared by the method according to any one of claims 1 to 7.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN113372148A (en) * | 2021-07-22 | 2021-09-10 | 创想未来生物工程(北京)有限公司 | Organic material decomposing microbial agent and preparation method thereof |
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