CN112159776A - 一种提升耕地肥力的复合酶制剂及其制备方法 - Google Patents

一种提升耕地肥力的复合酶制剂及其制备方法 Download PDF

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CN112159776A
CN112159776A CN202011051883.0A CN202011051883A CN112159776A CN 112159776 A CN112159776 A CN 112159776A CN 202011051883 A CN202011051883 A CN 202011051883A CN 112159776 A CN112159776 A CN 112159776A
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parts
enzyme preparation
cultivated land
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CN112159776B (zh
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卢姣姣
李建勇
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Fujian Luhai Ecological Technology Center Co ltd
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Abstract

本发明公开一种提升耕地肥力的复合酶制剂及其制备方法,该复合酶制剂包括如下组分:蔗糖酶、蛋白酶、淀粉酶、纤维素酶,果胶酶、木聚糖酶、磷酸酶、植酸酶、脲酶、脱氢酶、芳基硫酸酯酶、丝状酵母菌、根霉菌、多粘放线菌、胶冻样芽孢杆菌、戊糖片球菌和保水剂。通过以上多种生物酶和微生物菌剂混合后得到复合酶制剂。再将该复合酶制剂与有机肥复配施用至农田耕地时,可显著改善土壤理化性质,疏松土壤,改善板结现象,提高土壤pH,增加土壤有机质、有效氮磷钾和有益菌含量,提高耕地肥力,促使农作物的生长和农作物果实品质的提高。

Description

一种提升耕地肥力的复合酶制剂及其制备方法
技术领域
本发明涉及土壤改良技术领域,具体涉及一种提升耕地肥力的复合酶制剂及其制备方法。
背景技术
随着现代科学技术的发展,我国农业逐渐开始由传统农业向现代农业过渡,新品种、新技术以及化肥、农药等化学物资不断在农业生产上应用,虽然达到增加粮食作物产量的目的,但是却对土地和环境造成了不同程度的污染。对土地的不合理应用主要表现在重用地、轻养地,重产出、轻投入,重化肥、轻农肥、忽视微肥、不用菌肥,从而造成土壤板结、盐碱化、水体富营养化等环境问题。而且过量使用化肥会削弱作物生产能力,容易倒伏,导致减产;容易发生病虫害,使作物抗病虫能力减弱,易遭病虫侵害。
生物酶作为一种特殊的以蛋白质形式存在的生物催化剂,在食品、饲料等行业得到了广泛的应用,并因其特异、高效、绿色环保等特点而备受青睐。土壤酶是土壤中起生化反应的各种酶的总称,研究表明,土壤酶活力与土壤肥力、土壤理化性质密切相关,改善土壤酶活力可以在一定程度上提高耕地土壤肥力、改善土壤性状,从而提高作物产量。而目前,用于改善耕地土壤性质的生物酶成分单一,种类有限,且大多效果不佳,对土壤的改善和农作物的生长促进效果有限。
发明内容
本发明的目的在于提供一种提升耕地肥力的生物酶及其制备方法。通过采用多种生物酶和微生物菌剂混合后得到复合酶制剂。再将该复合酶制剂与有机肥复配施用至农田耕地时,可显著改善土壤理化性质,疏松土壤,改善板结现象,提高土壤pH,增加土壤有机质、有效氮磷钾和有益菌含量,提高耕地肥力,促使农作物的生长和农作物果实品质的提高。
为了实现上述目的,本发明所采用的技术方案是:
一方面,提供了一种提升耕地肥力的复合酶制剂,包括如下重量份的组分:蔗糖酶6~12份、蛋白酶6~10份、淀粉酶6~10份、纤维素酶5~10份、果胶酶2~8份、木聚糖酶3~6份、磷酸酶2~8份、植酸酶3~8份、脲酶4~8份、脱氢酶2~6份、芳基硫酸酯酶1~5份、丝状酵母菌3~5份、根霉菌2~5份、多粘放线菌1~5份、胶冻样芽孢杆菌1~5份、戊糖片球菌2~5份和保水剂0.5~5份。
优选地,包括如下重量份的组分:蔗糖酶8~10份、蛋白酶6~9份、淀粉酶6~8份、纤维素酶6~8份、果胶酶2~6份、木聚糖酶3~5份、磷酸酶2~6份、植酸酶4~6份、脲酶5~7份、脱氢酶3~5份、芳基硫酸酯酶2~4份、丝状酵母菌2~4份、根霉菌3~5份、多粘放线菌2~4份、胶冻样芽孢杆菌1~3份、戊糖片球菌2~4份和保水剂1~2份。
优选地,还包括如下重量份的组分:海藻多糖3~8份和螺旋藻0.5~3份。
优选地,还包括如下重量份的组分:海藻多糖4~6份和螺旋藻1~2份。
优选地,保水剂为MP3005。
更优选地,MP3005保水剂为MP3005K4、MP3005KL、MP3005KM、MP3005KB、MP3005S、MP3005KCE中的一种或几种。
优选地,丝状酵母菌含量为6~8×107cfu/g、根霉菌含量为2~4×107cfu/g、多粘放线菌含量为5~7×107cfu/g、胶冻样芽孢杆菌含量为6~8×107cfu/g、戊糖片球菌含量为4~6×107cfu/g。
另一方面,提供了一种提升耕地肥力的复合酶制剂的制备方法:按配比,将各组分混匀后,定量包装即得。
再一方面,提供了一种提升耕地肥力的复合酶制剂时应用于耕地种植时,与有机肥充分混合均匀,在种植农作物前施撒在土壤表面,再翻入土壤中,一年施撒一次。
优选地,复合酶制剂与有机肥混合时,以每亩耕地添加复合酶制剂100g~500g。
有益效果:
本发明复合酶制剂中各种酶组分可促进作物对土壤氮、磷的吸收和利用,增强了作物的光合作用,促进作物健康生长,并且不会造成土壤的污染,还能够缓解氮元素和磷元素在土壤中的累积,在维持土壤体系平衡,提高土壤生物学性能等方面中发挥着重要作用。
本发明复合酶制剂中各种微生物菌可降解土壤中部分有机质,一方面直接为植物生长提供一部分营养物质,另一方面也为土壤中微生物提供营养物质,促进微生物生长代谢,而微生物的代谢产物能为农作物提供更为丰富的营养物质。
因此,该复合酶制剂在微生物和酶催化的共同作用下,可以增加土壤有机质含量和多种生物活性物质,改善土壤物理、化学和生物学性状,提高土壤肥力,缓解土壤板结状况,提高土壤pH值、增加作物产量,而且能够改善农产品品质,能够促进农作物生长和品质改善,安全高效,可获得显著的社会和经济效益。
具体实施方式
下面结合实施例对本发明做进一步的描述,有必要在此指出的是以下实施例只是用于对本发明进行进一步的说明,不能理解为对本发明保护范围的限制,该领域的技术熟练人员根据上述发明内容所做出的一些非本质的改进和调整,仍属于本发明的保护范围。
实施例1
1.一种提升耕地肥力的复合酶制剂,包括如下组分:蔗糖酶9份、蛋白酶8份、淀粉酶7份、纤维素酶7份,果胶酶4份、木聚糖酶4份、磷酸酶3份、植酸酶5份、脲酶6份、脱氢酶4份、芳基硫酸酯酶3份、丝状酵母菌3份、根霉菌4份、多粘放线菌3份、胶冻样芽孢杆菌2份、戊糖片球菌3份和保水剂2份。
2.一种提升耕地肥力的复合酶制剂的制备方法,包括如下步骤:
(1)微生物菌粉的制备
丝状酵母菌含量为7×107cfu/g、根霉菌的含量为3×107cfu/g、多粘放线菌的含量为6×107cfu/g、胶冻样芽孢杆菌的含量为8×107cfu/g、戊糖片球菌的含量为5×107cfu/g。
步骤一、活化
将丝状酵母菌、根霉菌、多粘放线菌、胶冻样芽孢杆菌和戊糖片球菌分别接种于液体培养基中,35℃静置培养24小时,分别得到充分活化的丝状酵母菌、根霉菌、多粘放线菌、胶冻样芽孢杆菌和戊糖片球菌。
培养基的配方为:胰蛋白胨10g,酵母提取物5g,氯化钠50g,水1L。
步骤二、微生物菌粉制备
将步骤一充分活化的各菌种,分别接种于培养基中,控制培养温度为35℃,150rpm振荡培养48小时,再接种于含有与种子发酵相同的液体培养基的发酵罐中,发酵条件同种子发酵,发酵液分别在8000rpm离心10min,收集离心后的沉淀菌体,添加海藻糖后在-80℃冷冻干燥处理,制备成各微生物菌粉。
(2)复合酶制剂的制备
称取各组分,混合均匀后,按100g/包包装即得。
实施例2
1.一种提升耕地肥力的复合酶制剂,包括如下组分:蔗糖酶9份、蛋白酶8份、淀粉酶7份、纤维素酶7份,果胶酶4份、木聚糖酶4份、磷酸酶3份、植酸酶5份、脲酶6份、脱氢酶4份、芳基硫酸酯酶3份、丝状酵母菌3份、根霉菌4份、多粘放线菌3份、胶冻样芽孢杆菌2份、戊糖片球菌3份、保水剂2份、海藻多糖5份和螺旋藻1份。
2.一种提升耕地肥力的复合酶制剂的制备方法,包括如下步骤:
(1)微生物菌粉的制备
丝状酵母菌含量为6×107cfu/g、根霉菌的含量为2×107cfu/g、多粘放线菌的含量为7×107cfu/g、胶冻样芽孢杆菌的含量为8×107cfu/g、戊糖片球菌的含量为4×107cfu/g。
步骤一、活化
将丝状酵母菌、根霉菌、多粘放线菌、胶冻样芽孢杆菌和戊糖片球菌分别接种于液体培养基中,35℃静置培养24小时,分别得到充分活化的丝状酵母菌、根霉菌、多粘放线菌、胶冻样芽孢杆菌和戊糖片球菌。
培养基的配方为:胰蛋白胨10g,酵母提取物5g,氯化钠50g,水1L。
步骤二、微生物菌粉制备
将步骤一充分活化的各菌种,分别接种于培养基中,控制培养温度为35℃,150rpm振荡培养48小时,再接种于含有与种子发酵相同的液体培养基的发酵罐中,发酵条件同种子发酵,发酵液分别在8000rpm离心10min,收集离心后的沉淀菌体,添加海藻糖后在-80℃冷冻干燥处理,制备成各微生物菌粉。
(2)复合酶制剂的制备
称取各组分,混合均匀后,按100g/包包装即得。
实施例3
1.一种提升耕地肥力的复合酶制剂,包括如下组分:蔗糖酶12份、蛋白酶6份、淀粉酶6份、纤维素酶5份,果胶酶8份、木聚糖酶6份、磷酸酶2份、植酸酶3份、脲酶8份、脱氢酶6份、芳基硫酸酯酶4份、丝状酵母菌3份、根霉菌5份、多粘放线菌3份、胶冻样芽孢杆菌1份、戊糖片球菌1份和保水剂4份。
2.一种提升耕地肥力的复合酶制剂的制备方法,包括如下步骤:
(1)微生物菌粉的制备
丝状酵母菌含量为8×107cfu/g、根霉菌的含量为2×107cfu/g、多粘放线菌的含量为5×107cfu/g、胶冻样芽孢杆菌的含量为7×107cfu/g、戊糖片球菌的含量为4×107cfu/g。
步骤一、活化
将丝状酵母菌、根霉菌、多粘放线菌、胶冻样芽孢杆菌和戊糖片球菌分别接种于液体培养基中,35℃静置培养24小时,分别得到充分活化的丝状酵母菌、根霉菌、多粘放线菌、胶冻样芽孢杆菌和戊糖片球菌。
培养基的配方为:胰蛋白胨10g,酵母提取物5g,氯化钠50g,水1L。
步骤二、微生物菌粉制备
将步骤一充分活化的各菌种,分别接种于培养基中,控制培养温度为35℃,150rpm振荡培养48小时,再接种于含有与种子发酵相同的液体培养基的发酵罐中,发酵条件同种子发酵,发酵液分别在8000rpm离心10min,收集离心后的沉淀菌体,添加海藻糖后在-80℃冷冻干燥处理,制备成各微生物菌粉。
(2)复合酶制剂的制备
称取各组分,混合均匀后,按100g/包包装即得。
实施例4
1.一种提升耕地肥力的复合酶制剂,包括如下组分:蔗糖酶12份、蛋白酶6份、淀粉酶6份、纤维素酶5份,果胶酶8份、木聚糖酶6份、磷酸酶2份、植酸酶3份、脲酶8份、脱氢酶6份、芳基硫酸酯酶4份、丝状酵母菌3份、根霉菌5份、多粘放线菌3份、胶冻样芽孢杆菌1份、戊糖片球菌1份和保水剂4份、海藻多糖6份和螺旋藻0.5份。
2.一种提升耕地肥力的复合酶制剂的制备方法,包括如下步骤:
(1)微生物菌粉的制备
丝状酵母菌含量为7×107cfu/g、根霉菌的含量为4×107cfu/g、多粘放线菌的含量为5×107cfu/g、胶冻样芽孢杆菌的含量为6×107cfu/g、戊糖片球菌的含量为6×107cfu/g、海藻多糖6份和螺旋藻0.5份。
步骤一、活化
将丝状酵母菌、根霉菌、多粘放线菌、胶冻样芽孢杆菌和戊糖片球菌分别接种于液体培养基中,35℃静置培养24小时,分别得到充分活化的丝状酵母菌、根霉菌、多粘放线菌、胶冻样芽孢杆菌和戊糖片球菌。
培养基的配方为:胰蛋白胨10g,酵母提取物5g,氯化钠50g,水1L。
步骤二、微生物菌粉制备
将步骤一充分活化的各菌种,分别接种于培养基中,控制培养温度为35℃,150rpm振荡培养48小时,再接种于含有与种子发酵相同的液体培养基的发酵罐中,发酵条件同种子发酵,发酵液分别在8000rpm离心10min,收集离心后的沉淀菌体,添加海藻糖后在-80℃冷冻干燥处理,制备成各微生物菌粉。
(2)复合酶制剂的制备
称取各组分,混合均匀后,按100g/包包装即得。
实施例5
采用实施例1-4制备的复合酶制剂,用于耕地种植。即在整地施入基肥之前,将本发明产品与有机肥充分混合均匀,每亩种植地添加本产品100克,施撒100天后,检测土壤,之后开始种植番茄;
分组:将耕地划分成5个区域,分别为实施例1、实施例2、实施例3、实施例4和空白对照组(只含有机肥)。
(1)施加复合酶制剂对土壤性质的影响
施撒100天后,检测土壤,其结果如下表1所示:
表1复合酶制剂对土壤性质的影响结果
区域 外观 pH值 有益菌(个/g)
实施例1 疏松,无板结 6.3 2.0×10<sup>7</sup>
实施例2 疏松,无板结 6.1 2.1×10<sup>7</sup>
实施例3 疏松,无板结 6.0 1.9×10<sup>7</sup>
实施例4 疏松,无板结 6.0 1.8×10<sup>7</sup>
空白对照组 土壤紧实,有板结 4.6 未检出
续表1
Figure BDA0002709816990000071
从上表1可知,施用含实施例1-4制备的复合酶制剂的有机肥,施撒100天后,土壤性质得到了较大改善,土壤疏松、无板结现象,pH值符合耕地种植标准,有益菌达2.1×107个/g,有机质、碱解氮、有机磷、有机钾得到了大幅度提升,可恢复到有机种植的土壤标准,适合农作物种植。结果表明,施用实施例1-4复合酶制剂的土壤相比于空白对照组各个数据均有明显提高,说明本发明的复合复合酶制剂各种酶和微生物不仅能够很好地在土壤中存活并发挥其解磷解钾功能。
(2)施加复合酶制剂对农作物生长情况的影响
施撒复合酶制剂100天后,开始种植番茄,观察和记录农作物生长情况,结果如下表2所示:
表2施加复合酶制剂对农作物生长情况的影响结果
Figure BDA0002709816990000072
Figure BDA0002709816990000081
从上表2可知,施用含实施例1-4制备的复合酶制剂的有机肥,较空白对照组,实施例1-4种植得到的番茄生长形式良好,根量发达,茎秆加粗,坐果量浓密,果实大小增大,且实施例2效果最佳,说明海藻多糖和螺旋藻对农作物的生长起到了协同增效的作用。
(3)施加复合酶制剂对果实品质的影响
对番茄的果实品质及营养成分含量进行检测,结果如下表3所示:
表3施加复合酶制剂对果实品质的影响
Figure BDA0002709816990000082
从上表3可知,施用含实施例1-4制备的复合酶制剂的有机肥,较空白对照组,番茄果实的出汁率从71.3%提高至80%以上、Vc含量从23.1mg/kg提高至29.6mg/kg,总酸从1.08mg/kg降至0.84mg/kg,糖酸比从5.67提升至8.87,显著高于空白对照组,且实施例2效果最佳,说明海藻多糖和螺旋藻对果实品质起到了协同增效的作用。
因此,从以上复合酶制剂的施加对耕地土壤、农作物生长和果实品质的影响,可知施加本发明的复合酶制剂,可显著改善土壤理化性质,疏松土壤,提高土壤pH,增加土壤有机质、有效氮磷钾和有益菌含量,提高耕地肥力,起到减肥增效作用,提高农作物对矿质营养的吸收利用,从而促使农作物的生长和农作物果实品质的提高。

Claims (10)

1.一种提升耕地肥力的复合酶制剂,其特征在于,包括如下重量份的组分:蔗糖酶6~12份、蛋白酶6~10份、淀粉酶6~10份、纤维素酶5~10份、果胶酶2~8份、木聚糖酶3~6份、磷酸酶2~8份、植酸酶3~8份、脲酶4~8份、脱氢酶2~6份、芳基硫酸酯酶1~5份、丝状酵母菌3~5份、根霉菌2~5份、多粘放线菌1~5份、胶冻样芽孢杆菌1~5份、戊糖片球菌2~5份和保水剂0.5~5份。
2.根据权利要求1所述的一种提升耕地肥力的复合酶制剂,其特征在于,包括如下重量份的组分:蔗糖酶8~10份、蛋白酶6~9份、淀粉酶6~8份、纤维素酶6~8份、果胶酶2~6份、木聚糖酶3~5份、磷酸酶2~6份、植酸酶4~6份、脲酶5~7份、脱氢酶3~5份、芳基硫酸酯酶2~4份、丝状酵母菌2~4份、根霉菌3~5份、多粘放线菌2~4份、胶冻样芽孢杆菌1~3份、戊糖片球菌2~4份和保水剂1~2份。
3.根据权利要求1所述的一种提升耕地肥力的复合酶制剂,其特征在于,还包括如下重量份的组分:海藻多糖3~8份和螺旋藻0.5~3份。
4.根据权利要求3所述的一种提升耕地肥力的复合酶制剂,其特征在于,还包括如下重量份的组分:海藻多糖4~6份和螺旋藻1~2份。
5.根据权利要求1所述的一种提升耕地肥力的复合酶制剂,其特征在于,所述保水剂为MP3005。
6.根据权利要求5所述的一种提升耕地肥力的复合酶制剂,其特征在于,所述MP3005保水剂为MP3005K4、MP3005KL、MP3005KM、MP3005KB、MP3005S、MP3005KCE中的一种或几种。
7.根据权利要求1所述的一种提升耕地肥力的复合酶制剂,其特征在于,所述丝状酵母菌含量为6~8×107cfu/g、根霉菌含量为2~4×107cfu/g、多粘放线菌含量为5~7×107cfu/g、胶冻样芽孢杆菌含量为6~8×107cfu/g、戊糖片球菌含量为4~6×107cfu/g。
8.根据权利要求1所述的一种提升耕地肥力的复合酶制剂,其特征在于,所述复合酶制剂的制备方法:按配比,将各组分混匀后,定量包装即得。
9.根据权利要求1所述的一种提升耕地肥力的复合酶制剂,其特征在于,所述复合酶制剂应用于耕地种植时,与有机肥充分混合均匀,在种植农作物前施撒在土壤表面,再翻入土壤中,一年施撒一次。
10.根据权利要求9所述的一种提升耕地肥力的复合酶制剂,其特征在于,所述复合酶制剂与有机肥混合时,以每亩耕地添加复合酶制剂100g~500g。
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