CN112156060A - Propolis wash-free long-acting antibacterial gel and preparation method thereof - Google Patents
Propolis wash-free long-acting antibacterial gel and preparation method thereof Download PDFInfo
- Publication number
- CN112156060A CN112156060A CN202011263811.2A CN202011263811A CN112156060A CN 112156060 A CN112156060 A CN 112156060A CN 202011263811 A CN202011263811 A CN 202011263811A CN 112156060 A CN112156060 A CN 112156060A
- Authority
- CN
- China
- Prior art keywords
- propolis
- extract
- ethanol
- parts
- wash
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 241000241413 Propolis Species 0.000 title claims abstract description 55
- 229940069949 propolis Drugs 0.000 title claims abstract description 55
- 230000000844 anti-bacterial effect Effects 0.000 title claims abstract description 26
- 238000002360 preparation method Methods 0.000 title claims abstract description 21
- 238000001879 gelation Methods 0.000 title description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 96
- NIXOWILDQLNWCW-UHFFFAOYSA-N Acrylic acid Chemical compound OC(=O)C=C NIXOWILDQLNWCW-UHFFFAOYSA-N 0.000 claims abstract description 28
- 229920002125 Sokalan® Polymers 0.000 claims abstract description 28
- 229960001631 carbomer Drugs 0.000 claims abstract description 28
- 239000003906 humectant Substances 0.000 claims abstract description 20
- 229940069521 aloe extract Drugs 0.000 claims abstract description 19
- GSEJCLTVZPLZKY-UHFFFAOYSA-N Triethanolamine Chemical compound OCCN(CCO)CCO GSEJCLTVZPLZKY-UHFFFAOYSA-N 0.000 claims abstract description 14
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims description 32
- PUPZLCDOIYMWBV-UHFFFAOYSA-N (+/-)-1,3-Butanediol Chemical compound CC(O)CCO PUPZLCDOIYMWBV-UHFFFAOYSA-N 0.000 claims description 24
- 241000246044 Sophora flavescens Species 0.000 claims description 20
- 239000000843 powder Substances 0.000 claims description 20
- 238000003756 stirring Methods 0.000 claims description 13
- 235000011187 glycerol Nutrition 0.000 claims description 12
- 125000003976 glyceryl group Chemical group [H]C([*])([H])C(O[H])([H])C(O[H])([H])[H] 0.000 claims description 12
- 239000000203 mixture Substances 0.000 claims description 12
- 238000005406 washing Methods 0.000 claims description 12
- 238000001035 drying Methods 0.000 claims description 11
- 238000005325 percolation Methods 0.000 claims description 10
- 238000010298 pulverizing process Methods 0.000 claims description 10
- 238000002156 mixing Methods 0.000 claims description 8
- 239000002245 particle Substances 0.000 claims description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 7
- 238000000034 method Methods 0.000 claims description 6
- 238000002791 soaking Methods 0.000 claims description 6
- 239000012153 distilled water Substances 0.000 claims description 5
- 238000011049 filling Methods 0.000 claims description 5
- 239000000706 filtrate Substances 0.000 claims description 5
- 238000001914 filtration Methods 0.000 claims description 5
- 239000002904 solvent Substances 0.000 claims description 5
- 239000006228 supernatant Substances 0.000 claims description 5
- 238000007599 discharging Methods 0.000 claims description 4
- 239000008187 granular material Substances 0.000 claims description 4
- 230000006641 stabilisation Effects 0.000 claims description 4
- 238000011105 stabilization Methods 0.000 claims description 4
- 238000005303 weighing Methods 0.000 claims description 4
- 238000010992 reflux Methods 0.000 claims description 2
- 241000894006 Bacteria Species 0.000 abstract description 7
- 230000002147 killing effect Effects 0.000 abstract description 4
- 230000002045 lasting effect Effects 0.000 abstract description 3
- 239000003205 fragrance Substances 0.000 abstract description 2
- 239000002085 irritant Substances 0.000 abstract description 2
- 231100000021 irritant Toxicity 0.000 abstract description 2
- 231100000252 nontoxic Toxicity 0.000 abstract 1
- 230000003000 nontoxic effect Effects 0.000 abstract 1
- 239000000499 gel Substances 0.000 description 21
- 230000000052 comparative effect Effects 0.000 description 12
- 238000012360 testing method Methods 0.000 description 12
- 230000003385 bacteriostatic effect Effects 0.000 description 6
- 238000002474 experimental method Methods 0.000 description 4
- 244000000010 microbial pathogen Species 0.000 description 4
- 230000003020 moisturizing effect Effects 0.000 description 4
- 229920001817 Agar Polymers 0.000 description 3
- 235000009161 Espostoa lanata Nutrition 0.000 description 3
- 240000001624 Espostoa lanata Species 0.000 description 3
- 239000008272 agar Substances 0.000 description 3
- 238000004140 cleaning Methods 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 230000000813 microbial effect Effects 0.000 description 3
- 244000005700 microbiome Species 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- 230000000638 stimulation Effects 0.000 description 3
- 208000035473 Communicable disease Diseases 0.000 description 2
- 239000001888 Peptone Substances 0.000 description 2
- 108010080698 Peptones Proteins 0.000 description 2
- 206010040880 Skin irritation Diseases 0.000 description 2
- 235000015278 beef Nutrition 0.000 description 2
- 239000000645 desinfectant Substances 0.000 description 2
- 230000010355 oscillation Effects 0.000 description 2
- 235000019319 peptone Nutrition 0.000 description 2
- 230000001681 protective effect Effects 0.000 description 2
- 230000036556 skin irritation Effects 0.000 description 2
- 231100000475 skin irritation Toxicity 0.000 description 2
- 238000004659 sterilization and disinfection Methods 0.000 description 2
- 241001116389 Aloe Species 0.000 description 1
- 241000222122 Candida albicans Species 0.000 description 1
- 102000008186 Collagen Human genes 0.000 description 1
- 108010035532 Collagen Proteins 0.000 description 1
- 201000004624 Dermatitis Diseases 0.000 description 1
- 241000588724 Escherichia coli Species 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 239000004909 Moisturizer Substances 0.000 description 1
- 241000191967 Staphylococcus aureus Species 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- 235000011399 aloe vera Nutrition 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 230000003110 anti-inflammatory effect Effects 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 229940095731 candida albicans Drugs 0.000 description 1
- 229920001436 collagen Polymers 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 230000000249 desinfective effect Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 210000001145 finger joint Anatomy 0.000 description 1
- 244000053095 fungal pathogen Species 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 230000007794 irritation Effects 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000001333 moisturizer Effects 0.000 description 1
- 210000004877 mucosa Anatomy 0.000 description 1
- 238000011056 performance test Methods 0.000 description 1
- 230000008929 regeneration Effects 0.000 description 1
- 238000011069 regeneration method Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000037307 sensitive skin Effects 0.000 description 1
- 230000037067 skin hydration Effects 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 210000004243 sweat Anatomy 0.000 description 1
- 230000002195 synergetic effect Effects 0.000 description 1
- 230000017423 tissue regeneration Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/98—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin
- A61K8/987—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin of species other than mammals or birds
- A61K8/988—Honey; Royal jelly, Propolis
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/02—Cosmetics or similar toiletry preparations characterised by special physical form
- A61K8/04—Dispersions; Emulsions
- A61K8/042—Gels
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
- A61K8/34—Alcohols
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
- A61K8/34—Alcohols
- A61K8/345—Alcohols containing more than one hydroxy group
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/84—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds obtained by reactions otherwise than those involving only carbon-carbon unsaturated bonds
- A61K8/86—Polyethers
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9794—Liliopsida [monocotyledons]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q17/00—Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
- A61Q17/005—Antimicrobial preparations
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/10—Washing or bathing preparations
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/70—Biological properties of the composition as a whole
- A61K2800/72—Hypo-allergenic
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Veterinary Medicine (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Birds (AREA)
- Epidemiology (AREA)
- Mycology (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Botany (AREA)
- Microbiology (AREA)
- Emergency Medicine (AREA)
- Dermatology (AREA)
- Chemical & Material Sciences (AREA)
- Dispersion Chemistry (AREA)
- Zoology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Cosmetics (AREA)
Abstract
The invention provides a propolis wash-free long-acting antibacterial gel and a preparation method thereof. The propolis wash-free long-acting antibacterial gel comprises 95% ethanol, propolis extract, radix Sophorae Flavescentis extract, Aloe extract, carbomer solution, triethanolamine, and humectant. The gel disclosed by the invention can be used for instantly killing various common bacteria, repairing surface skin, keeping moisture for a long time, and being fresh and cool in fragrance, non-toxic, non-irritant, lasting and efficient.
Description
Technical Field
The invention belongs to the technical field of daily chemical products, and particularly relates to a propolis wash-free long-acting antibacterial gel and a preparation method thereof.
Background
The hands are most easily contacted with various pathogenic microorganisms such as bacteria, viruses and the like, and if the pathogenic microorganisms cannot be timely and effectively removed, the pathogenic microorganisms can survive for hours at palms, finger joints, fingertips and the like and even can continue to grow and reproduce to become sites for hiding and reproducing the pathogenic microorganisms. A great deal of research shows that the hand cleaning and disinfecting can effectively eliminate the occurrence of various infectious diseases and prevent the spread of the infectious diseases. The washing-free gel can inhibit and remove bacteria on hands on the premise of not using clear water for cleaning, is convenient to carry, and is widely applied.
However, most of the currently marketed leave-on gels have difficulty in achieving a lasting bacteriostatic effect, and are added with an irritant component, causing hand discomfort after use.
Disclosure of Invention
In order to solve the problems of the no-wash gel in the prior art, the invention provides the no-wash long-acting antibacterial propolis gel and the preparation method thereof, which can kill bacteria instantly, inhibit bacteria permanently, are safe and have no stimulation.
The invention provides a propolis wash-free long-acting antibacterial gel, which comprises 95% ethanol, a propolis extract, a radix sophorae flavescentis extract, an aloe extract, a carbomer solution, triethanolamine and a humectant; the preparation method of the propolis extract comprises the following steps: pulverizing frozen propolis into powder, adding 60-70% ethanol, reflux extracting at a temperature lower than 90 deg.C and 8-10 times of ethanol, stirring, filtering to obtain supernatant, washing with ethanol, adding the washing solution into the filtrate, concentrating, drying, and storing in dark place.
Preferably, the formula comprises the following components in parts by weight: 60-80 parts of 95% ethanol, 1-5 parts of propolis extract, 0.5-1 part of radix sophorae flavescentis extract, 0.5-1.5 parts of aloe extract, 10-20 parts of carbomer solution, 0.1-1 part of triethanolamine and 1-3 parts of humectant.
Particularly preferably, the formula comprises the following components in parts by weight: 70 parts of 95% ethanol, 3 parts of propolis extract, 0.75 part of radix sophorae flavescentis extract, 1 part of aloe extract, 15 parts of carbomer solution, 0.5 part of triethanolamine and 2 parts of humectant.
Preferably, the preparation method of the sophora flavescens extract comprises the following steps: pulverizing radix Sophorae Flavescentis, adding 70% ethanol to submerge radix Sophorae Flavescentis powder, soaking for 22-26 hr; then filling the sophora flavescens powder and the soak solution into a percolator, wherein the percolation solvent is 70% ethanol, the percolation speed is 4-6 ml/(kg. min), collecting the percolate with 6 times of the volume of the sophora flavescens powder, concentrating and drying to obtain the sophora flavescens extract.
Preferably, the preparation method of the carbomer solution comprises the following steps: carbomer is weighed and added into distilled water with the volume of 20-30 times, and soaked for 12-24 hours.
Preferably, the aloe extract is a commercially available 100:1 extract.
Preferably, the humectant comprises glycerin, glyceryl polyether-26 and 1.3-butanediol.
Preferably, the mass ratio of the glycerol to the glyceryl polyether-26 to the 1.3-butanediol is 1.5-2.5:4-6: 2-4.
Particularly preferably, the mass ratio of the glycerol to the glyceryl polyether-26 to the 1.3-butanediol is 2:5: 3.
The invention also provides a preparation method of the propolis wash-free long-acting antibacterial gel, which comprises the following steps:
(1) weighing carbomer solution according to the formula ratio;
(2) adding triethanolamine regulator to regulate pH to 6.5-7.5;
(3) adding 95% ethanol according to the formula amount, adding the mixture in three times, and stirring and mixing the mixture for each time until the mixture is completely uniform and has no particles;
(4) adding a humectant in a formula amount, and uniformly mixing until no particles exist;
(5) sequentially adding propolis extract, aloe extract and radix Sophorae Flavescentis extract, and stirring to completely uniform without granule;
(6) homogenizing for 8-12 min at 2000-2500rpm, and discharging after stabilization.
The invention has the following beneficial effects:
the high-alcohol contained in the gel has good instant killing effect on escherichia coli, staphylococcus aureus, candida albicans, common pathogenic bacteria and pathogenic fungi in hospitals; the contained propolis extract forms a protective film on the surface of skin, inhibits bacterial regeneration for a long time, has the specific tissue repair function and anti-inflammatory effect of propolis, and has good effect on repairing superficial skin inflammation and mucosa; the active ingredients of the bitter leaves and the aloe and the humectant form a synergistic disinfection system and a skin moisturizing system with the propolis and the ethanol, so that the disinfection effect is ensured and the hand skin is protected. The gel disclosed by the invention can be used for instantly killing various common bacteria, repairing surface skin, keeping moisture for a long time, and is fresh and cool in fragrance, safe, free of stimulation, lasting and efficient.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
The propolis wash-free long-acting antibacterial gel comprises the following components in parts by mass: 60 parts of 95% ethanol, 1 part of propolis extract, 0.5 part of radix sophorae flavescentis extract, 0.5 part of aloe extract, 10 parts of carbomer solution, 0.1 part of triethanolamine and 1 part of humectant; the humectant comprises glycerin, glyceryl polyether-262 and 1.3-butanediol, and the mass ratio of the glycerin to the glyceryl polyether-26 to the 1.3-butanediol is 1.5:4: 2.
The preparation method of the propolis extract comprises the following steps: pulverizing frozen propolis into powder, adding 60% ethanol, reflux-extracting at a temperature lower than 90 deg.C and 8 times of ethanol, stirring, filtering to obtain supernatant, washing with ethanol, adding the washing solution into the filtrate, concentrating, drying, and storing in dark place.
The preparation method of the sophora flavescens extract comprises the following steps: pulverizing radix Sophorae Flavescentis, adding 70% ethanol to submerge radix Sophorae Flavescentis powder, soaking for 22 hr; then filling the sophora flavescens powder and the soak solution into a percolator, wherein the percolation solvent is 70% ethanol, the percolation speed is 4 ml/(kg. min), collecting the percolate with 6 times of the volume of the sophora flavescens powder, concentrating and drying to obtain the sophora flavescens extract.
The preparation method of the carbomer solution comprises the following steps: carbomer was soaked in 20 volumes of distilled water for 12 hours.
The aloe extract is a commercially available 100:1 extract.
Example 2
The propolis wash-free long-acting antibacterial gel comprises the following components in parts by mass: 70 parts of 95% ethanol, 3 parts of propolis extract, 0.75 part of radix sophorae flavescentis extract, 1 part of aloe extract, 15 parts of carbomer solution, 0.5 part of triethanolamine and 2 parts of humectant; the humectant comprises glycerol, glyceryl polyether-262 and 1.3-butanediol, and the mass ratio of the glycerol to the glyceryl polyether-26 to the 1.3-butanediol is 2:5: 3.
The preparation method of the propolis extract comprises the following steps: pulverizing frozen propolis into powder, adding 65% ethanol, reflux-extracting at a temperature lower than 90 deg.C and 9 times of ethanol, stirring, filtering to obtain supernatant, washing with ethanol, adding the washing solution into the filtrate, concentrating, drying, and storing in dark place.
The preparation method of the sophora flavescens extract comprises the following steps: pulverizing radix Sophorae Flavescentis, adding 70% ethanol to submerge radix Sophorae Flavescentis powder, and soaking for 24 hr; then filling the sophora flavescens powder and the soak solution into a percolator, wherein the percolation solvent is 70% ethanol, the percolation speed is 5 ml/(kg. min), collecting the percolate with 6 times of the volume of the sophora flavescens powder, concentrating and drying to obtain the sophora flavescens extract.
The preparation method of the carbomer solution comprises the following steps: carbomer was soaked in 25 volumes of distilled water for 18 hours.
The aloe extract is a commercially available 100:1 extract.
Example 3
The propolis wash-free long-acting antibacterial gel comprises the following components in parts by mass: 80 parts of 95% ethanol, 5 parts of propolis extract, 1 part of radix sophorae flavescentis extract, 1.5 parts of aloe extract, 20 parts of carbomer solution, 1 part of triethanolamine and 3 parts of humectant; the humectant comprises glycerol, glyceryl polyether-262 and 1.3-butanediol, and the mass ratio of the glycerol to the glyceryl polyether-26 to the 1.3-butanediol is 2.5:6: 4.
The preparation method of the propolis extract comprises the following steps: pulverizing frozen propolis into powder, adding 70% ethanol, reflux-extracting at a temperature lower than 90 deg.C with 10 times of ethanol, stirring, filtering to obtain supernatant, washing with ethanol, adding the washing solution into the filtrate, concentrating, drying, and storing in dark place.
The preparation method of the sophora flavescens extract comprises the following steps: pulverizing radix Sophorae Flavescentis, adding 70% ethanol to submerge radix Sophorae Flavescentis powder, soaking for 26 hr; then filling the sophora flavescens powder and the soak solution into a percolator, wherein the percolation solvent is 70% ethanol, the percolation speed is 6 ml/(kg. min), collecting the percolate with 6 times of the volume of the sophora flavescens powder, concentrating and drying to obtain the sophora flavescens extract.
The preparation method of the carbomer solution comprises the following steps: carbomer was soaked in 30 volumes of distilled water for 24 hours.
The aloe extract is a commercially available 100:1 extract.
Example 4
A method for preparing propolis wash-free long-acting antibacterial gel comprises the following steps:
(1) weighing carbomer solution according to the formula ratio;
(2) adding a formula amount of triethanolamine regulator into the carbomer solution to adjust the pH to 6.5;
(3) adding 95% ethanol according to the formula amount, adding the mixture in three times, and stirring and mixing the mixture for each time until the mixture is completely uniform and has no particles;
(4) adding a humectant in a formula amount, and uniformly mixing until no particles exist;
(5) sequentially adding propolis extract, aloe extract and radix Sophorae Flavescentis extract, and stirring to completely uniform without granule;
(6) homogenizing for 8 minutes at 2500rpm, and discharging after stabilization.
Example 5
A method for preparing propolis wash-free long-acting antibacterial gel comprises the following steps:
(1) weighing carbomer solution according to the formula ratio;
(2) adding triethanolamine regulator in formula amount into carbomer solution to adjust pH to 7.5;
(3) adding 95% ethanol according to the formula amount, adding the mixture in three times, and stirring and mixing the mixture for each time until the mixture is completely uniform and has no particles;
(4) adding a humectant in a formula amount, and uniformly mixing until no particles exist;
(5) sequentially adding propolis extract, aloe extract and radix Sophorae Flavescentis extract, and stirring to completely uniform without granule;
(6) homogenizing for 12 minutes at 2000rpm, and discharging after stabilization.
Comparative example 1
Commercial 75% ethanol leave-on hand gels.
Comparative example 2
The commercial 75% ethanol disinfectant.
In order to verify the long-acting antibacterial property, the safety property and the moisturizing property of the no-wash gel, the following tests were respectively carried out, and the samples supplied in the tests were example 1, example 2, example 3, comparative example 1 and comparative example 2.
Experiment 1 Long-acting bacteriostatic test
Respectively taking 3g of samples to wipe the skin of the back of the hand of a testee, exposing the samples for corresponding time under the same environment, adopting an aseptic operation method, using an aseptic cotton ball to wipe the drug application part of the back of the hand, washing the cotton ball in 10ml of aseptic water for 5min by oscillation, taking 0.1ml of oscillation washing liquid, culturing the cotton ball in a beef extract peptone solid culture medium and a PDA agar plate by a pouring method for certain time, and carrying out long-acting bacteriostatic experiments on bacteria and fungi to obtain long-acting bacteriostatic experiment results, wherein the long-acting bacteriostatic experiment results are respectively shown in a table 1 and a table 2:
TABLE 1 beef extract peptone agar plate bacteriostatic test results
TABLE 2 PDA agar plate results of bacteriostasis test
| 3min | 60min | 240min | 300min | |
| Example 1 | - | - | - | + |
| Example 2 | - | - | - | + |
| Example 3 | - | - | - | + |
| Comparative example 1 | - | + | ++ | +++ |
| Comparative example 2 | - | + | ++ | +++ |
Wherein: "-" represents no microbial growth;
"+" represents microbial colony count < 5/dish;
"+ +" represents microbial colony count < 20/dish;
"+ + + +" indicates that the colony count of microorganisms < 100/dish or colony clumping failed to count.
As can be seen from the above experimental results, the killing and inhibiting effects on microorganisms by several test articles in a short time are almost the same. However, the propolis ethanol hand-washing-free gel shows an excellent long-acting antibacterial effect with the antibacterial time of 4 hours. The main reason is that the ethanol solution added with the propolis has certain hydrophobicity, and a protective film can be formed on the surface of skin, so that the breeding of microorganisms can be effectively inhibited.
Test 2 irritation test results (crowd test)
100 subjects were randomly selected, divided into 4 groups on average, and 5 samples were taken in portions of 3g each. The samples of comparative example 1 were applied to the left hand of 100 subjects as controls, and the samples of example 1 were applied to the right hand of the first group of subjects, the samples of example 2 were applied to the right hand of the second group of subjects, the samples of example 3 were applied to the right hand of the third group of subjects, and the samples of comparative example 2 were applied to the right hand of the fourth group of subjects. After it had dried, the degree of skin irritation to which the subject responded was recorded as shown in table 3:
TABLE 3 statistical Table of skin irritation levels of subjects
The experimental result shows that the propolis ethanol hand-washing-free gel has no stimulation to skin, and can be used for cleaning hands of infants and sensitive skin.
Test 3 moisture Retention Performance test results (crowd test)
100 subjects were randomly selected, divided into 4 groups on average, and 5 samples were taken in portions of 3g each. The samples of comparative example 1 were applied to the left hand of 100 subjects as controls, and the samples of example 1 were applied to the right hand of the first group of subjects, the samples of example 2 were applied to the right hand of the second group of subjects, the samples of example 3 were applied to the right hand of the third group of subjects, and the samples of comparative example 2 were applied to the right hand of the fourth group of subjects. After drying, the skin moisture content of the hand skin of the subject was measured by using a skin moisture meter, and the average sweat level of the hand of the subject using the same sample was counted, with the test results shown in table 4:
TABLE 4 statistical table of average hand skin hydration levels of subjects
| Example 1 | Example 2 | Example 3 | Comparative example 1 | Comparative example 2 | |
| Average moisture content of skin | 27.9％ | 28.3％ | 28.6％ | 19.6％ | 17.9％ |
It is apparent from the experimental results that the moisture content of the skin of the subject using the propolis ethanol no-wash gel of the present invention is significantly higher than the moisture content of the skin using 75% ethanol no-wash gel and 75% ethanol disinfectant. The reason is that propolis has a regeneration-promoting effect on collagen, which is the basis of skin maintaining moisture and elasticity. In addition, the moisturizer contained in the formula has an additional moisturizing effect on the skin. Therefore, the product has more excellent moisturizing performance than like products.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (10)
1. A propolis wash-free long-acting antibacterial gel is characterized in that: the formula comprises 95% ethanol, propolis extract, radix Sophorae Flavescentis extract, Aloe extract, carbomer solution, triethanolamine, and humectant; the preparation method of the propolis extract comprises the following steps: pulverizing frozen propolis into powder, adding 60-70% ethanol, reflux extracting at a temperature lower than 90 deg.C and 8-10 times of ethanol, stirring, filtering to obtain supernatant, washing with ethanol, adding the washing solution into the filtrate, concentrating, drying, and storing in dark place.
2. The propolis wash-free long-acting antibacterial gel according to claim 1, which is characterized in that: the formula comprises the following components in parts by mass: 60-80 parts of 95% ethanol, 1-5 parts of propolis extract, 0.5-1 part of radix sophorae flavescentis extract, 0.5-1.5 parts of aloe extract, 10-20 parts of carbomer solution, 0.1-1 part of triethanolamine and 1-3 parts of humectant.
3. The propolis wash-free long-acting antibacterial gel according to claim 2, which is characterized in that: the formula comprises the following components in parts by mass: 70 parts of 95% ethanol, 3 parts of propolis extract, 0.75 part of radix sophorae flavescentis extract, 1 part of aloe extract, 15 parts of carbomer solution, 0.5 part of triethanolamine and 2 parts of humectant.
4. The propolis leave-in long-acting antibacterial gel according to any one of claims 1 to 3, which is characterized in that: the preparation method of the sophora flavescens extract comprises the following steps: pulverizing radix Sophorae Flavescentis, adding 70% ethanol to submerge radix Sophorae Flavescentis powder, soaking for 22-26 hr; then filling the sophora flavescens powder and the soak solution into a percolator, wherein the percolation solvent is 70% ethanol, the percolation speed is 4-6 ml/(kg. min), collecting the percolate with 6 times of the volume of the sophora flavescens powder, concentrating and drying to obtain the sophora flavescens extract.
5. The propolis wash-free long-acting antibacterial gel according to claim 4, which is characterized in that: the preparation method of the carbomer solution comprises the following steps: adding carbomer into distilled water with volume 20-30 times of that of carbomer, and soaking for 12-24 hr.
6. The propolis wash-free long-acting antibacterial gel according to claim 1, which is characterized in that: the aloe extract is a commercially available 100:1 extract.
7. The propolis wash-free long-acting antibacterial gel according to claim 1, which is characterized in that: the humectant comprises glycerin, glyceryl polyether-26, and 1.3-butanediol.
8. The propolis wash-free long-acting antibacterial gel according to claim 7, which is characterized in that: the mass ratio of the glycerol to the glyceryl polyether-26 to the 1.3-butanediol is 1.5-2.5:4-6: 2-4.
9. The propolis wash-free long-acting antibacterial gel according to claim 8, which is characterized in that: the mass ratio of the glycerol to the glyceryl polyether-26 to the 1.3-butanediol is 2:5: 3.
10. A method for preparing the propolis leave-in long-acting antibacterial gel of any one of claims 1 to 9, which is characterized in that: the method comprises the following steps:
(1) weighing carbomer solution according to the formula ratio;
(2) adding triethanolamine with formula amount into carbomer solution to adjust pH to 6.5-7.5;
(3) adding 95% ethanol according to the formula amount, adding the mixture in three times, and stirring and mixing the mixture for each time until the mixture is completely uniform and has no particles;
(4) adding a humectant in a formula amount, and uniformly mixing until no particles exist;
(5) sequentially adding propolis extract, aloe extract and radix Sophorae Flavescentis extract, and stirring to completely uniform without granules;
(6) homogenizing for 8-12 min at 2000-2500rpm, and discharging after stabilization.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011263811.2A CN112156060A (en) | 2020-11-12 | 2020-11-12 | Propolis wash-free long-acting antibacterial gel and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011263811.2A CN112156060A (en) | 2020-11-12 | 2020-11-12 | Propolis wash-free long-acting antibacterial gel and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN112156060A true CN112156060A (en) | 2021-01-01 |
Family
ID=73865785
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202011263811.2A Pending CN112156060A (en) | 2020-11-12 | 2020-11-12 | Propolis wash-free long-acting antibacterial gel and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN112156060A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116059290A (en) * | 2021-11-01 | 2023-05-05 | 云南邦纳科技有限公司 | Washing-free disinfectant for kuh-seng plants and preparation process |
| CN116763999A (en) * | 2023-08-25 | 2023-09-19 | 四川大学 | Urinary system catheter using propolis alcohol extract as coating and preparation method thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20100099547A (en) * | 2009-03-03 | 2010-09-13 | (주)지원메디코스 | Antiallergic cleansing cosmetic composition and method of preparing thereof |
| CN108853409A (en) * | 2018-08-13 | 2018-11-23 | 西安中天生物医药有限公司 | A kind of propolis aloetic bacteriostatic gel and preparation method thereof |
| CN110251448A (en) * | 2019-06-28 | 2019-09-20 | 江苏蜂奥生物科技有限公司 | A kind of cosmetic compositions containing propolis extract |
| CN111514098A (en) * | 2020-05-21 | 2020-08-11 | 浙江红石梁健康科技有限公司 | Washing-free disinfection gel and preparation method thereof |
-
2020
- 2020-11-12 CN CN202011263811.2A patent/CN112156060A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20100099547A (en) * | 2009-03-03 | 2010-09-13 | (주)지원메디코스 | Antiallergic cleansing cosmetic composition and method of preparing thereof |
| CN108853409A (en) * | 2018-08-13 | 2018-11-23 | 西安中天生物医药有限公司 | A kind of propolis aloetic bacteriostatic gel and preparation method thereof |
| CN110251448A (en) * | 2019-06-28 | 2019-09-20 | 江苏蜂奥生物科技有限公司 | A kind of cosmetic compositions containing propolis extract |
| CN111514098A (en) * | 2020-05-21 | 2020-08-11 | 浙江红石梁健康科技有限公司 | Washing-free disinfection gel and preparation method thereof |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116059290A (en) * | 2021-11-01 | 2023-05-05 | 云南邦纳科技有限公司 | Washing-free disinfectant for kuh-seng plants and preparation process |
| CN116763999A (en) * | 2023-08-25 | 2023-09-19 | 四川大学 | Urinary system catheter using propolis alcohol extract as coating and preparation method thereof |
| CN116763999B (en) * | 2023-08-25 | 2023-11-07 | 四川大学 | Urinary system catheter using propolis alcohol extract as coating and preparation method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN109316536A (en) | A kind of skin mucosa disinfecting agent and preparation method thereof | |
| CN102273715B (en) | Common lophatherum herb extract natural food preservative and preparation method thereof | |
| CN107412585B (en) | Green environment-friendly hand-washing-free disinfection gel and preparation method thereof | |
| CN112156060A (en) | Propolis wash-free long-acting antibacterial gel and preparation method thereof | |
| CN107028848A (en) | A kind of disinfection solution of disposable hand natural green | |
| CN107970488A (en) | A kind of chitin quarternary ammonium salt aquagel antiseptic dressing and preparation method thereof | |
| CN113499294B (en) | Plant composition for cats with skin and hair care effects and application thereof | |
| CN109077945A (en) | Combined bacteriostat with lysozyme for cosmetics | |
| CN108354880A (en) | A kind of baby child's skin care compositions and methods | |
| CN111803624A (en) | Skin antibacterial gel preparation containing transfer factor and preparation method thereof | |
| CN114796047A (en) | Anti-dandruff composition, application of anti-dandruff composition, shampoo and preparation method of shampoo | |
| CN111184675A (en) | No-clean disinfection composition, preparation method thereof and no-clean disinfection gel | |
| CN101543658A (en) | Cervical cap for preventing and treating cervical erosion and preparation method thereof | |
| CN109010124B (en) | Marine refined gynecological antibacterial gel and preparation method thereof | |
| CN108969455B (en) | Acne-removing and moisturizing composition containing herba violae and application of acne-removing and moisturizing composition | |
| CN109568650A (en) | A kind of chitosan quaternary ammonium salt antiseptic dressing and preparation method thereof | |
| CN108852900A (en) | A kind of antibacterial facial mask and preparation method thereof containing tridecanoic peptide | |
| CN106074343B (en) | A kind of disposable hand cleanser of thymol nanoparticle and preparation method thereof | |
| CN107334689A (en) | A kind of heavy metal adsorption film | |
| CN114569473B (en) | Environment-friendly wash-free antibacterial gel and preparation method thereof | |
| CN108451799A (en) | A kind of hand cleanser and preparation method thereof | |
| CN114767561B (en) | Anti-dandruff shampoo composition | |
| CN110585081A (en) | Antibacterial and itching-relieving shampoo containing folium artemisiae argyi essential oil and preparation method thereof | |
| CN114306526B (en) | Compound broadleaf holly leaf traditional Chinese medicine extract and application thereof in preparing shower gel or hand sanitizer | |
| CN113456550B (en) | Plant composition for dogs with skin and hair care effect and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20210101 |
|
| RJ01 | Rejection of invention patent application after publication |