CN112154860A - Composite material for improving microbial diversity and inhibiting pathogenic bacteria and preparation method thereof - Google Patents

Abstract

The invention relates to a composite material for improving microbial diversity and inhibiting pathogenic bacteria and a preparation method thereof, wherein the composite material comprises the following raw material components in parts by weight: 90-110 parts of an inoculant, 0.5-1.5 parts of cyclodextrin and 1-3 parts of calcium peroxide, wherein the inoculant comprises the following raw material components in percentage by weight: 45-55% of peat soil, 15-25% of needle-broad mixed forest soil, 5-15% of active carbon, 1-3% of dry moss, 0.3-0.8% of fresh pine needles, 1-3% of fresh broad leaves, 3-8% of fresh shrub branches, 0.5-1.5% of fresh shrub leaves, 3-8% of dry pine barks, 0.3-0.8% of fresh usnea and 3-5% of dry corn straws. The composite material can be applied to landscaping materials (soil, sand and turf), inhibits pathogenic bacteria in the landscaping materials, improves the microbial community diversity in the landscaping materials, and achieves the purpose of optimizing the microbial community around the human body.

Description

Composite material for improving microbial diversity and inhibiting pathogenic bacteria and preparation method thereof

Technical Field

The invention relates to the field of garden materials, in particular to a composite material for improving microbial diversity and inhibiting pathogenic bacteria and a preparation method thereof.

Background

The current global urbanization process is rapid, the population is more and more dense, and the urban ecological environment pressure is aggravated. Pollution is increased and ecology is destroyed in densely populated cities, and then immune-mediated diseases and infectious diseases are easy to outbreak, and the common diseases comprise eczema, allergic dermatitis, tracheitis, pneumonia, tuberculosis and the like. Public health and disease prevention and control are under great pressure, and medical systems are overwhelmed. How to reduce "overboard" by means of ecological measures upstream of the river is a requirement of the disease control sector.

Environmental microorganisms are highly related to human health, however, the diversity and population structure of environmental microorganisms in densely populated urban areas are often eroded and destroyed. The change of ecological environment in densely populated areas destroys the microbial population which co-evolves with human beings, indirectly affects the human immune system, and causes immune-mediated diseases such as allergy, asthma, rhinitis and diabetes. The exposure of diversified natural environment microorganisms is helpful for the development and the health of the human immune system, and has an inhibiting effect on immune-mediated non-infectious diseases. Therefore, the technology for developing a garden material additive, improving the microbial diversity of the surrounding environment of the human body and improving the microbial community structure is important for establishing a perfect immune system of the human body and preventing and controlling diseases.

At present, garden product materials such as soil, sand and stones and lawns are not considered to strengthen the microbial diversity of human living environment, and the microbial community structure cannot be optimized.

Disclosure of Invention

The invention aims to solve the problems of poor diversity of environmental microbial communities and high proportion of pathogenic bacteria in the current garden materials, and provides a composite material for improving the diversity of microorganisms and inhibiting the pathogenic bacteria and a preparation method thereof.

A composite material for improving microbial diversity and inhibiting pathogenic bacteria comprises the following raw material components in parts by weight: 90-110 parts of an inoculant, 0.5-1.5 parts of cyclodextrin and 1-3 parts of calcium peroxide, wherein the inoculant comprises the following raw material components in percentage by weight: 45-55% of peat soil, 15-25% of needle-broad mixed forest soil, 5-15% of active carbon, 1-3% of dry moss, 0.3-0.8% of fresh pine needles, 1-3% of fresh broad leaves, 3-8% of fresh shrub branches, 0.5-1.5% of fresh shrub leaves, 3-8% of dry pine barks, 0.3-0.8% of fresh usnea and 3-5% of dry corn straws.

As a preferred technical scheme, the composite material for improving microbial diversity and inhibiting pathogenic bacteria comprises the following raw material components in parts by weight: 100 parts of an inoculant, 1 part of cyclodextrin and 2 parts of calcium peroxide; the inoculant comprises the following raw material components in percentage by weight: 50% of peat soil, 20% of coniferous and broad mixed forest soil, 10% of active carbon, 1-3% of dry moss, 0.3-0.8% of fresh pine needles, 1-3% of fresh broad leaves, 3-8% of fresh shrub branches, 0.5-1.5% of shrub fresh leaves, 3-8% of dry pine barks, 0.3-0.8% of fresh usnea and 3-5% of dry corn straws.

As a preferred technical scheme, the composite material for improving microbial diversity and inhibiting pathogenic bacteria comprises the following raw material components in parts by weight: 100 parts of an inoculant, 1 part of cyclodextrin and 2 parts of calcium peroxide; the inoculant comprises the following raw material components in percentage by weight: 50% of peat soil, 20% of soil of a coniferous and broad mixed forest, 10% of active carbon, 2% of moss, 0.5% of fresh pine needles, 2% of fresh broad leaves, 5% of fresh shrub branches, 1% of fresh shrub leaves, 5% of dried pine barks, 0.5% of fresh usnea and 4% of dry corn straws.

As a preferred technical scheme, the peat soil is landscaping soil.

Preferably, the needle-broad mixed forest soil is selected from needle-broad mixed forest surface soil.

As a preferable technical scheme, the activated carbon is coal granular activated carbon for efficient adsorption.

Preferably, the dry moss is dry moss for gardens, and the fresh broad leaves are selected from white birch or juglans mandshurica.

As a preferred technical scheme, the fresh shrub branches are clove shrub branches, and the fresh shrub leaves are clove leaves.

As a preferred technical scheme, the dry pine bark is the bark of a camphor tree, and the fresh usnea is the usnea koreana.

A preparation method of a composite material for improving microbial diversity and inhibiting pathogenic bacteria comprises the following steps:

(1) preparing an inoculant, preparing raw materials according to a ratio, crushing the materials into powder with the diameter less than 5mm by using a crusher, and uniformly mixing to obtain the inoculant;

(2) adding cyclodextrin and calcium peroxide into the inoculant, adjusting the humidity to 50 percent and the pH to 7 +/-0.5, composting at room temperature, and uniformly mixing to obtain the composite material.

The composite material can be applied to landscaping materials (soil, gravel and turf), and can be added into common landscaping materials (soil, gravel and turf) to improve microbial community diversity, inhibit bacteria such as staphylococcus aureus, sphingomonas, rosella, pneumococcus, legionella and fungi such as candida albicans, and the proportion of the added materials is as follows:

the volume ratio of the composite material to the landscaping soil is 20-30: 70-80, preferably 25: 75.

The volume ratio of the composite material to the sand is 8-12: 88-92, preferably 10: 90.

The turf adding proportion is as follows: 18-22 kg/square meter, preferably 20 kg/square meter.

The inoculant provided by the invention is a substance rich in natural soil microorganisms, which is produced by taking forest byproducts as raw materials through an inoculant reconstruction technology and a pathogenic bacterium inhibition technology. In the raw material components, forest soil, litters, moss and other forest production byproducts contain rich microbial resources. The forest litters naturally contain a large amount of organic matters which are difficult to degrade, such as lignin and phenolic substances, so that diversified microbial communities taking the organic matters as carbon sources are inoculated, and the microorganisms have good effect on hydrocarbon substances.

The invention has the mechanism that the aims of inhibiting pathogenic bacteria and performing innocent treatment are achieved on the basis of a microbial inoculant, the microbial inoculant can be added into garden materials, the diversity of microbial communities can be improved, the pathogenic bacteria can be inhibited, the consumption of easily degradable substances (such as saccharides, micromolecular organic matters and the like) in the inoculant by the microorganisms is accelerated by using a cyclodextrin surfactant, and the substances are beneficial to breeding the pathogenic bacteria; the composting process is started by adjusting the humidity and the pH value, the calcium peroxide is added to slowly release oxygen, pathogenic bacteria are prevented from being bred by anaerobic fermentation, the microbial inoculum is subjected to pathogenic bacteria control treatment in the previous step, the treated inoculant is added into garden materials and applied to human activity areas, the microbial community diversity and structure around human body can be improved, the microbial community diversity and structure of human skin, respiratory tract and intestinal tract can be adjusted, and according to the hypothesis of microbial diversity, the microbial community can help the human body to improve the immunity and inhibit various immune mediated diseases.

Detailed Description

The present invention is described in detail below with reference to specific examples, but the present invention is not limited thereto in any way.

Example 1

The raw material components used by the inoculant in the examples are specifically:

1. the peat soil for gardening, which is common landscaping soil, generally contains no plant seeds through composting technology (60-70 ℃ in high temperature stage, at least one hour).

2. Needle broad mixed forest top layer pine needle leaf miscella soil, this type soil is taken from needle broad mixed forest, and main kind of tree includes: pinus sylvestris, larch, birch, juglans mandshurica, quercus mongolica and the like, and comprises a complete withered layer and 10cm of surface soil.

3. Activated carbon: the coal granular active carbon for high-efficiency adsorption is adopted in GB/T7701.7-1997, and the particle aperture is as follows: 2 mm.

4. Dry moss: dry moss water for gardens.

5. Fresh pine needles: any kind of needle (the embodiment adopts the needle of the camphor pine).

6. Fresh broad leaves: the extract may be white birch, Juglans mandshurica and so on (in the example, white birch leaf is adopted).

7. Fresh shrub branches: any shrub branch (clove shrub branches are used in the embodiment).

8. Fresh shrub leaves: can be any shrub leaves (clove leaves are used in the examples).

9. Dried pine bark: may be any pine bark (in the example, Pinus sylvestris bark is used).

10. Fresh usnea: the usnea koreana is red pine.

11. Dry corn stalks: common agricultural dry corn stalks.

The preparation method comprises mixing the above materials at a certain ratio, pulverizing into powder with diameter less than 3mm, and mixing.

The specific formulation of the inoculant is detailed in table 1.

TABLE 1 EXAMPLE 1 concrete raw material ratios of inoculants

Components	Example 1
		Peat soil	50.0％
Needle-broad mixed forest soil	20.0％
		Activated carbon	10.0％
Dried moss	2.0％
		Fresh pine needle	0.5％
Fresh broad leaf	2.0％
		Fresh shrub branches	5.0％
Fresh leaf of shrub	1.0％
		Bark of Pinus densiflora	5.0％
Fresh usnea	0.5％
		Dry corn stalk	4.0％
Total up to	100％

Examples 2 to 4 and comparative examples 1 to 3

Examples 2-4 are for the preparation of composites, the inoculant of examples 2-4 was prepared as in example 1, the specific formulation is detailed in table 2, and the specific preparation method is: adding cyclodextrin and calcium peroxide into the inoculant, adjusting humidity to 50%, adjusting pH to 7 + -0.5, performing composting treatment (25 + -5 deg.C) for 2 weeks, preventing rain, and mixing completely.

TABLE 2 examples 2-4 and comparative examples 1-3 are the compounding ratio (parts by weight) of the raw materials for the composite material

Components	Example 2	Example 3	Example 4	Comparative example 1	Comparative example 2	Comparative example 3
							Inoculants	100	100	100	100	100	100
Cyclodextrin	1	0.5	1.5	-	1	-
							Calcium peroxide	2	1.0	3.0	2	-	-

Example 5

The following test examples 2 to 4 and comparative examples 1 to 3 show the specific application effects of the composite materials, and the specific method is as follows: adding 25 parts of the composite material into 75 parts of landscaping soil, fully mixing the two materials uniformly, applying the mixture to an outdoor environment, and detecting the abundance diversity index and the pathogenic bacteria number of the species, wherein the result is detailed in table 3.

Wherein, the microbial community species abundance detection adopts an Illumina Miseq high-throughput sequencing platform to analyze 16s rRNA genes of bacteria, the total number of the bacterial gene sequences is 15000, and the human infectious pathogenic bacteria index (sum) is calculated according to Picrusts software.

Table 3 examples 8-14 are results of the application effect test

Note: the species abundance diversity index is relative to the original garden soil without the addition of the composite material.

As shown in Table 3, the composite materials prepared in examples 2-4 can significantly improve the microbial community of garden soil, increase the microbial diversity and reduce the pathogenic bacteria index, and the addition of cyclodextrin and calcium peroxide can significantly inhibit the pathogenic bacteria index.

Example 6

The composite material prepared in the example 1 is mixed with quartz sand to prepare the quartz sand which can improve the diversity of microorganisms on human skin and reduce the relative proportion of various pathogenic bacteria, 10 parts of the composite material is added into 90 parts of the quartz sand, and then the two are fully mixed.

Washing the fingers of both hands with clear water, putting the fingers of the left hand into the quartz sand added with the inoculant, putting the fingers of the right hand into the common quartz sand, standing for 2 minutes, and taking out the fingers. The experimental results are shown in table 4, and it can be seen that when the finger is contacted with the quartz sand containing the inoculant, the diversity index of skin bacteria is improved by 240.16% compared with the common quartz sand. Bacteria of the genera Acinetobacter, Burkholderia, Chlorobacter, Corynebacterium, Pseudomonas, Ralstonia, Rossinia, Sphingomonas, Staphylococcus are the main potential pathogens in the common Quartz Sand (relative ratio relative to relative abundance > 1%), the proportion of which in the modified Quartz Sand is significantly reduced.

The microbial community species abundance detection in the example adopts an Illumina Miseq high-throughput sequencing platform to analyze 16s rRNA genes of bacteria, and the total number of the obtained bacterial gene sequences in each experimental unit is 15000.

TABLE 4 results of the experiment

Note: the percentages in the table are relative proportions of the bacterial 16s rRNA genes (relative aborlance).

The embodiments described above are described to facilitate an understanding and use of the invention by those skilled in the art. It will be readily apparent to those skilled in the art that various modifications to these embodiments may be made, and the generic principles described herein may be applied to other embodiments without the use of the inventive faculty. Therefore, the present invention is not limited to the above embodiments, and those skilled in the art should make improvements and modifications within the scope of the present invention based on the disclosure of the present invention.

Claims (10)

1. The composite material for improving microbial diversity and inhibiting pathogenic bacteria is characterized by comprising the following raw material components in parts by weight: 90-110 parts of an inoculant, 0.5-1.5 parts of cyclodextrin and 1-3 parts of calcium peroxide, wherein the inoculant comprises the following raw material components in percentage by weight: 45-55% of peat soil, 15-25% of needle-broad mixed forest soil, 5-15% of active carbon, 1-3% of dry moss, 0.3-0.8% of fresh pine needles, 1-3% of fresh broad leaves, 3-8% of fresh shrub branches, 0.5-1.5% of fresh shrub leaves, 3-8% of dry pine barks, 0.3-0.8% of fresh usnea and 3-5% of dry corn straws.

2. The composite material for improving microbial diversity and inhibiting pathogenic bacteria according to claim 1 is characterized by comprising the following raw material components in parts by weight: 100 parts of an inoculant, 1 part of cyclodextrin and 2 parts of calcium peroxide;

the inoculant comprises the following raw material components in percentage by weight: 50% of peat soil, 20% of coniferous and broad mixed forest soil, 10% of active carbon, 1-3% of dry moss, 0.3-0.8% of fresh pine needles, 1-3% of fresh broad leaves, 3-8% of fresh shrub branches, 0.5-1.5% of shrub fresh leaves, 3-8% of dry pine barks, 0.3-0.8% of fresh usnea and 3-5% of dry corn straws.

3. The composite material for improving microbial diversity and inhibiting pathogenic bacteria according to claim 1 is characterized by comprising the following raw material components in parts by weight: 100 parts of an inoculant, 1 part of cyclodextrin and 2 parts of calcium peroxide;

the inoculant comprises the following raw material components in percentage by weight: 50% of peat soil, 20% of soil of a coniferous and broad mixed forest, 10% of active carbon, 2% of moss, 0.5% of fresh pine needles, 2% of fresh broad leaves, 5% of fresh shrub branches, 1% of fresh shrub leaves, 5% of dried pine barks, 0.5% of fresh usnea and 4% of dry corn straws.

4. The composite material for improving microbial diversity and inhibiting pathogenic bacteria according to any one of claims 1 to 3, wherein the peat soil is landscaping soil.

5. A composite material for promoting microbial diversity and for suppressing pathogenic bacteria as claimed in any one of claims 1 to 3, wherein said pin-wide commingled forest soil is selected from pin-wide commingled forest topsoil.

6. The composite material for improving microbial diversity and inhibiting pathogenic bacteria according to any one of claims 1 to 3, wherein the activated carbon is coal-based granular activated carbon for high-efficiency adsorption.

7. A composite material for increasing microbial diversity and for suppressing pathogenic bacteria as claimed in any one of claims 1 to 3 wherein said dry moss is dry moss for gardens and said fresh broad leaf is selected from birch or juglans mandshurica.

8. A composite material for improving microbial diversity and inhibiting pathogenic bacteria according to any one of claims 1 to 3, wherein said fresh shrub branches are clove shrub branches and said shrub fresh leaves are clove leaves.

9. The composite material for improving microbial diversity and inhibiting pathogenic bacteria of any one of claims 1-3, wherein said dried pine bark is Pinus sylvestris and said fresh Usnea is Usnea koraiensis.

10. A method of preparing a composite material for increasing microbial diversity and inhibiting pathogenic bacteria according to any one of claims 1 to 3, comprising the steps of:

(1) preparing an inoculant, preparing raw materials according to a ratio, crushing the materials into powder with the diameter less than 5mm by using a crusher, and uniformly mixing to obtain the inoculant;

(2) adding cyclodextrin and calcium peroxide into the inoculant, adjusting the humidity to 50 percent and the pH to 7 +/-0.5, composting at room temperature, and uniformly mixing to obtain the composite material.