CN112141483A

CN112141483A - Container and method of using same

Info

Publication number: CN112141483A
Application number: CN202010857063.4A
Authority: CN
Inventors: 章卫东; 张文芳; 梁爱民; 徐洪海
Original assignee: Renolit Hengxun Packaging Technology Beijing Co ltd
Current assignee: Renolit Hengxun Packaging Technology Beijing Co ltd
Priority date: 2020-08-24
Filing date: 2020-08-24
Publication date: 2020-12-29
Also published as: WO2022041402A1

Abstract

The invention discloses a container and a using method thereof, wherein the container comprises: first container is both ends open-ended cavity barrel, has relative first end and second end, and inside is provided with the separator, separates the barrel for first cavity and second cavity, and movably sets up the combined cover of first end, includes: the cover body is provided with a window, the second container is movably and hermetically arranged at the second end, a third cavity is formed in the second container, one side, away from the second end, of the third cavity is a blocking end, a first sealing piece is arranged at one side, close to the second end, of the third cavity, the puncture device is internally provided with at least one channel communicated with the third cavity, the separation piece and the first sealing piece can be punctured by the puncture device in the process that the second container moves towards the side close to the first container, and a first reagent in the first cavity can flow into the third cavity through the channel. The invention can simplify the operation flow, improve the testing efficiency, test the safety, reduce the cost and is not limited by the testing field.

Description

Container and method of using same

Technical Field

The invention relates to the technical field of biochemistry, in particular to a container and a using method thereof.

Background

In the field of biochemistry today, it is desirable to add one reagent to another in a sealed environment to facilitate subsequent performance of specific biological tests, chemical experiments, and the like. Among them, a typical one of the testing techniques in the biological test is an in vitro diagnostic technique. Among the in vitro diagnostic techniques, PCR (Polymerase Chain Reaction) is one of the currently very important diagnostic techniques.

In a specific application scenario, the PCR in vitro diagnosis technology can be used for nucleic acid detection of viruses. The current general testing process is divided into: reagent preparation, nucleic acid extraction, sample application and PCR amplification.

At present, when the PCR in-vitro diagnosis technology is applied to virus nucleic acid detection, a swab sample is firstly packaged by using a PCR in-vitro diagnosis kit on a sampling site, then the kit packaged with the swab sample is sent to a negative pressure biological laboratory, the swab sample is inactivated, then the kit is opened, then reagent preparation is carried out, a prepared reagent is added into the inactivated swab sample for nucleic acid extraction, then a reaction solution is added into a reagent for extracting nucleic acid, then the reagent is placed into a PCR instrument for treatment, and finally a detection result is obtained.

The inventor finds that the detection process has the following problems:

1. the swab sample needs to be inactivated after being obtained, then the prepared reagent is used for extracting nucleic acid, and the reaction solution is added for testing, so that the operation is complex, and in the operation process, certain infection risk exists in operators.

2. The core operation process of the detection process needs to be operated in a negative pressure biological laboratory and a biological safety cabinet, so that the cost is high, and the detection process is limited by sites.

3. The whole testing process has multiple operation steps, long testing time and low testing efficiency.

Disclosure of Invention

To overcome at least one of the deficiencies in the prior art, the present invention provides a container and method of use thereof.

The above object of the present invention can be achieved by the following technical solutions:

a container, comprising: the device comprises a first container, a second container and a third container, wherein the first container is integrally a hollow cylinder with two open ends, the cylinder is provided with a first end and a second end which are opposite to each other, a movable partition is arranged in the cylinder, the partition divides the cylinder into a first cavity and a second cavity, and a first reagent is contained in the first cavity; a composite cover movably disposed at said first end, said composite cover comprising: the cover body is provided with a window, when the cover body is positioned at a first position relative to the cylinder body, the window is in an open state, when the cover body is positioned at a second position relative to the cylinder body, the window is in a closed state, and the combined cover seals the first end; the second container is movably and hermetically arranged at the second end, a third cavity for containing a second reagent is formed in the second container, one side of the third cavity, which is far away from the second end, is a blocking end, and a first sealing element is arranged close to the second end; and a puncture device disposed in the first container or the second container, wherein at least one through passage is formed in the puncture device, the puncture device can puncture the partition and the first sealing member during the process that the second container moves toward the first container, and the first reagent in the first cavity can flow into the third cavity through the passage.

In a preferred embodiment, the second container has an initial position of abutment with respect to the first container during movement of the second container to the side adjacent the first container, the second container has a piercing position with respect to the first container when the piercer pierces the first seal and the partition, and the partition is stationary with respect to the first container before the piercer pierces the first seal and the partition; after the puncture outfit punctures the first sealing element and the separating element, the second container drives the separating element to move continuously for a preset distance to reach a termination position, and a preset amount of the first reagent drops into the third cavity through the channel.

In a preferred embodiment, a limiting part for limiting the position of the partition or the first sealing element along the axial direction of the cylinder is arranged on the inner wall of the first container.

In a preferred embodiment, the piercer is disposed in the second container, and the first sealing member is provided with a crown portion for sealing a side of the second cavity close to the second end, and a plug portion extending into the third cavity and sealing a position where the piercer is engaged with the third cavity.

In a preferred embodiment, a first external thread is arranged on the outer side wall of the cylinder body close to the second end, and a first internal thread matched with the first external thread is arranged on the inner side wall of the second container.

In a preferred embodiment, a first accommodating part for installing the puncture outfit and a second accommodating part for accommodating a second reagent are arranged in the third cavity, a third accommodating part is arranged on the periphery of the first accommodating part, the first internal thread is arranged on the third accommodating part, and a clearance structure is formed on one side of the third accommodating part, which is far away from the first internal thread.

In a preferred embodiment, the first seal further comprises a protective sheath attached to the crown, the protective sheath being disposed over the penetration portion.

In a preferred embodiment, the cover body is a hollow structure with two open ends, and has a bottom end relatively close to the second end and a top end relatively far away from the second end, and the combined cover further includes a second sealing element disposed at the top end, and a fastener pressed on the second sealing element.

In a preferred embodiment, the engaging member is annular, a first engaging portion is disposed on an outer side wall of the engaging member, a first engaging groove engaged with the first engaging portion is disposed on an inner wall of the cover body near the top end, and the first engaging portion and the first engaging groove cooperate to form an engaging structure for fixing the second sealing member.

In a preferred embodiment, the composite cover is at least axially movable relative to the first container to switch the open or closed state of the window.

In a preferred embodiment, a first matching portion is arranged on the outer wall of the cylinder, a second matching portion is arranged on the inner wall of the cover body, and when the cover body is located at a second position relative to the cylinder, the first matching portion and the second matching portion are matched to form a locking structure.

In a preferred embodiment, the first engaging portion includes a plurality of second engaging portions that are spaced apart from each other in a circumferential direction of the cylinder or in an axial direction of the cylinder, and the second engaging portion is a plurality of second engaging portions that are spaced apart from each other in the circumferential direction of the cover.

In a preferred embodiment, a third engaging portion is further disposed on an outer wall of the barrel close to the first end, when the second engaging portion engages with the third engaging portion, the cover is located at a farthest position away from the second end, and the window is in an open state.

In a preferred embodiment, the first engaging portion is a plurality of second engaging grooves spaced around the circumference of the cylinder, and the third engaging portion is an annular protruding rib.

In a preferred embodiment, the first fitting portion includes: the two second clamping grooves are at least arranged along the axial direction of the barrel at intervals, and the through part is communicated with the two second clamping grooves; the third clamping part is two buckles arranged at intervals.

In a preferred embodiment, the partition 13 includes any one of: a rubber plug and a piston.

In a preferred embodiment, when the separator is a rubber plug, the whole rubber plug is arranged in the barrel body in an interference fit mode, and a part to be punctured is arranged in the middle of the rubber plug; the limiting part is positioned on one side of the rubber plug close to the first end, after the second container moves for a first distance close to the limiting part, the puncture outfit punctures the part to be punctured limited by the limiting part, and then the second container can drive the rubber plug to move for a second distance to the first end after crossing the limiting part; or, when the partition is a piston, the piston includes: a tip center is provided with the main part of portion of waiting to pierce through to and the cover is established sealing ring on the main part outer wall, the sealing ring with barrel interference fit, spacing position in the piston is close to one side of second end, the second container remove first distance extremely with after spacing portion contacts, the puncture ware pierces through wait to pierce through the portion, the second container can cross spacing portion drives the piston again to first end removes the second distance.

In a preferred embodiment, the puncture outfit is disposed in the first container, the puncture outfit comprises a puncture part and a base at one end of the puncture part, the base is in sealing fit with the barrel, and the partition is sleeved outside the puncture part.

In a preferred embodiment, the outer side wall of the second container is provided with a second external thread, and the inner side wall of the first container close to the first end is provided with a second internal thread matched with the second external thread.

A use method of the container comprises the following steps:

moving the cover body to a first position in a direction departing from the second end of the cylinder body, opening the window, placing a sample containing a measured object into the first container through the window, moving the cover body to a second position close to the second end of the cylinder body, and closing the window; the sample is contacted with a first reagent contained in the first container;

and during the process that the second container is arranged at the second end and the second container is close to the first end, the puncture outfit punctures the separating piece and the first sealing piece and then drops a preset volume of the first reagent into the second reagent of the second container.

In a preferred embodiment, the second container has an initial position of abutment with respect to the first container during movement of the second container to the side adjacent the first container, the second container has a piercing position with respect to the first container when the piercer pierces the first seal and the partition, and the partition is stationary with respect to the first container before the piercer pierces the partition and the first seal; after the puncture outfit punctures the first sealing element and the separating element, the second container drives the separating element to move for a preset distance to reach a termination position, and a preset amount of the first reagent is dripped into the third cavity through the channel.

In a preferred embodiment, the puncture location is spaced a second distance from the termination location, the second distance being determined based on the volume and cross-sectional flow area of the first cavity, the volume and viscosity of the first reagent, the target amount of the first reagent required for testing, and the cross-sectional flow area of the puncture instrument.

The container that provides in the embodiment of this application has the first container of separator through the setting, movably sets up the combined cover at the first end of first container, movably sealed the second container that sets up at first container second end, and the optimal design of the inner structure of first container and second container, can be used for realizing carrying out the flow that the nucleic acid drawed immediately after the sample sampling, the problem that the test must go on in biological laboratory, biological safety operation cabinet has been solved, the security risk has been reduced, the detection efficiency is improved, shorten detection latency greatly, reduce inspection cost.

In addition, the function of quantitatively delivering the first reagent in the first container to the second container is realized through reasonable control of the motion state of the separating part and reasonable design of the compression of the air of the closed cavity in the first container and the passage of the puncture outfit. In specific use, the separating component is kept static relative to the first container before being punctured, and moves for a specific distance after being pushed by a specific force after being punctured, so that a preset amount of the first reagent is squeezed into the second container from the first container, and accurate addition of the first reagent in a fixed amount into the second reagent is realized without operation of a professional.

In general, when the container provided by the embodiment of the application is used for carrying out biological tests, chemical experiments and the like in a closed environment, for example, virus extraction and detection, the traditional diagnostic test process can be completely changed, and effective technical support is provided for realizing rapid and instant detection.

Drawings

The invention is further described with reference to the following figures and embodiments.

FIG. 1 is a schematic diagram of a container according to an embodiment of the present disclosure;

FIG. 2 is a schematic view of the container of FIG. 1 in an initial position of a second container relative to the first container;

FIG. 3 is a schematic view of the container of FIG. 1 with a second container in a piercing position relative to the first container;

FIG. 4 is a schematic view of the container of FIG. 1 in an end position of the second container relative to the first container;

FIG. 5 is a schematic view of a first container of the container provided in FIG. 1;

FIG. 6 is a cross-sectional view of the first container of FIG. 5;

FIG. 7 is a cross-sectional view of the composite closure of the container provided in FIG. 1;

FIG. 8 is an exploded cross-sectional view of a second container of the container provided in FIG. 1;

FIG. 9 is a schematic illustration of a first container of yet another container provided in an embodiment of the present application;

FIG. 10 is a schematic diagram of another container configuration provided in an embodiment of the present application;

FIG. 11 is a schematic view of the container of FIG. 10 shown with the second container in a piercing position relative to the first container;

FIG. 12 is a schematic view of the container of FIG. 10 in an end position of the second container relative to the first container;

fig. 13 is an exploded view of a first container of the type provided in fig. 10.

Description of reference numerals:

100. a first container; 11. a first end; 12. a second end; 110. a first cavity; 120. a second cavity; 13. a separator; 14. a limiting part; 15. a first external thread; 16. a first mating portion; 17. a third engaging portion; 18. a second internal thread;

200. a second container; 20. a third cavity; 201. a first accommodating portion; 202. a second accommodating portion; 203. a third accommodating portion; 21. a puncture instrument; 211. a puncture section; 212. a base; 213. opening a hole; 214. a seal ring; 22. a first seal member; 221. a crown portion; 222. a plug portion; 223. a protective sleeve; 23. a first internal thread; 24. a void-avoiding structure; 25. a second external thread;

300. a combined cover; 30. a cover body; 301. a window; 302. a first card slot; 303. a second mating portion; 31. a second seal member; 32. a fastener; 320. a first engaging portion.

Detailed Description

The technical solutions of the present invention will be described in detail with reference to the accompanying drawings and specific embodiments, it should be understood that these embodiments are merely illustrative of the present invention and are not intended to limit the scope of the present invention, and various equivalent modifications of the present invention by those skilled in the art after reading the present invention fall within the scope of the appended claims.

It will be understood that when an element is referred to as being "disposed on" another element, it can be directly on the other element or intervening elements may also be present. When an element is referred to as being "connected" to another element, it can be directly connected to the other element or intervening elements may also be present. The terms "vertical," "horizontal," "upper," "lower," "left," "right," and the like as used herein are for illustrative purposes only and do not denote a unique embodiment.

Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs. The terminology used herein in the description of the present application is for the purpose of describing particular embodiments only and is not intended to be limiting of the application. As used herein, the term "and/or" includes any and all combinations of one or more of the associated listed items.

In the embodiment of the application, a brand-new container and a using method thereof are provided aiming at scenes such as nucleic acid detection of some viruses, microorganism inoculation tests or toxic chemical reagent mixing tests, so that the operation process can be simplified, the testing efficiency can be improved, the testing safety can be improved, the cost can be reduced, and the limitation of a testing site is avoided.

Referring to fig. 1 to 10 in combination, in an embodiment of the present disclosure, a container is provided, which mainly includes: a first container 100, a composite cover 300, and a second container 200.

In the present embodiment, the first container 100 is a hollow cylinder having both ends open. The barrel has opposite first and second ends 11 and 12. In the present embodiment, as shown in fig. 6 as an example, the first end 11 is an opposite upper end, and the second end 12 is an opposite lower end. A partition 13 is provided inside the cylinder. The partition 13 partitions the cartridge into a first chamber 110 and a second chamber 120. The first chamber 110 is for containing a first reagent therein.

In this embodiment, the combination cover 300 is movably disposed at the first end 11 of the cylinder. The composite cover 300 may include: a cover 30 provided with a window 301. When the cover 30 is in the first position relative to the barrel, the window 301 is in the open state, and when the cover 30 is in the second position relative to the barrel, the window 301 is in the closed state and the combination cover 300 seals the first end 11.

In this embodiment, the second container 200 is movably and sealingly disposed at the second end 12 of the barrel. A third chamber 20 for containing a second reagent is formed in the second container 200. The side of the third chamber 20 facing away from the second end 12 of the barrel is a sealed end, and a puncture device 21 is disposed on the side close to the second end 12 of the barrel. The puncture instrument 21 has at least one passage formed therein which communicates with the third chamber 20.

During the movement of the second container 200 to the side close to the first container 100, the puncture instrument 21 can puncture the separation member 13, and the first reagent in the first cavity 110 can flow into the third cavity 20 through the passage of the puncture instrument 21.

Wherein, the specific component of the first reagent can be different according to different use scenes of the container. For example, when the container is used in the context of an in vitro diagnostic test, the first reagent may be a lytic reagent or a so-called lytic reagent for extracting biological features (including DNA, RNA) in the virus; when the container is applied to a microbial inoculation test scene, the first reagent can be a microbial inoculum; when the container is used in a mixing test scenario for toxic chemical reagents, the first reagent may be one that requires mixing. Since the first reagent needs to be injected into the third cavity 20, the first reagent may be a fluid, for example, a liquid, a solid-liquid mixture, or the like.

The specific composition of the second reagent may also vary depending on the use scenario of the container. For example, when the container is used in the context of an in vitro diagnostic test, the second reagent may be a test agent for testing the biological characteristics of the lysis of the lysing agent; when the container is applied to a microbial inoculation test scene, the second reagent can be a culture solution; when the container is used in a mixing test scenario for toxic chemical reagents, the second reagent may be another reagent that needs to be mixed. Specifically, the second reagent may be a solid, a liquid, a solid-liquid mixture, or the like.

In a specific application scenario, when the container provided by the embodiment of the present application is used for virus detection, the operation steps are as follows: the sample after the completion of the sample collection is first put into the first container 100. After the sample enters the first container 100, the combined cover 300 locks the first container 100 tightly and is not opened any more; the first container 100 is then oscillated to ensure that the sample is sufficiently wetted with the first reagent to facilitate reliable extraction of the nucleic acid; then, the first container 100 and the second container 200 are connected, the second container 200 is pushed a distance relative to the first container 100, the piercer 21 in the second container 200 can pierce the partition 13, and after the partition 13 is pierced, the partition 13 is pushed by the second container 200 to move a predetermined distance, so that a prescribed dose of the first reagent is dropped into the second reagent. And finally, placing the container into a tester to test the read data.

The whole testing process is in the inclosed environment, and the tester does not have the risk of exposing, has improved the security of test greatly, does not have the requirement to outside test environment moreover, need not to adopt dedicated negative pressure biological laboratory, greatly reduced the cost, improved the convenience of test, in addition, also cancelled the deactivation, transported steps such as swab sample, improved efficiency of software testing greatly.

Referring to fig. 7, in the present embodiment, the second container 200 may further include a first sealing member 22. The first sealing member 22 is provided with a crown portion 221 for sealing the side of the second chamber 120 adjacent the second end 12 of the barrel, and a plug portion 222 extending into the third chamber 20 and sealing the engagement position of the piercer 21 with the third chamber 20.

In this embodiment, the first seal 22 may be formed in a hollow ring shape as a whole, and the hollow portion thereof is a through hole for sealingly passing the puncture instrument 21 therethrough. The annular portion includes a crown 221 and a plug 222, wherein the crown 221 has an outer diameter greater than an outer diameter of the plug 222.

The first sealing member 22 is disposed in the second container 200, and when the second container 200 is connected to the first container 100, the crown 221 of the first sealing member 22 can be in sealing engagement with the second cavity 120, thereby preventing the reagent from leaking out from the engagement position of the first container 100 and the second container 200. The plug 222 is hermetically provided between the second container 200 and the puncture instrument 21, and prevents the reagent from leaking from the position where the two are engaged.

In this embodiment, the puncture instrument 21 may have a hollow structure as a whole, and at least one passage communicating with the third chamber 20 is formed inside the puncture instrument 21. The number of the channels may be one or more.

Specifically, the puncture instrument 21 may include: a puncturing part 211 and a base 212 located at one end of the puncturing part 211. The plug portion 222 is provided in a gap between the puncture portion 211 and the first housing portion 201. One end of the plug 222 abuts against the base 212 and the other end connects with the crown 221.

The puncture part 211 of the puncture instrument 21 is provided at a tip end thereof with at least one opening 213. The number of channels depends on the number of openings 213. When the number of the openings 213 is one, the number of the channels is one, and when the number of the openings 213 is two, the number of the channels is two. The base 212 of the spike 21 cooperates with a corresponding portion of the second container 200 to ensure that the spike 21 remains stable and non-askew during use.

To protect the second reagent in the second container 200 from leakage and to ensure sealing before use, the first sealing member 22 may further comprise a protective sleeve 223 attached to the crown 221. The protective cover 223 is fitted over the puncture part 211 and can seal the puncture part 211 of the puncture device 21.

Generally, in the field of virus detection, the ratio of the first reagent to the second reagent required for detection has specific requirements. Wherein the second reagent placed in the third chamber 20 can be set to a fixed known amount, and at this time, the first reagent required for detection is also limited to a predetermined range. For example, for the detection of a certain virus, the volume of the first reagent required may be about 40. mu.l, or about 100. mu.l. How to accurately add the target amount of the first reagent in the sealed state into the second reagent in the third cavity 20 by using the container provided in the present application is a problem to be solved.

Generally, one way to achieve the addition of a predetermined amount of reagent to another reagent in a sealed state is to: using special kit, for example, a liquid gun with precision scales for pipetting, obviously this method is not suitable for this application. Another way is to arrange the cylinder of the first container 100 in a deformable structure, which cannot guarantee that the first reagent is added in a target amount by squeezing the first container 100 to make the first reagent in the first container 100 flow into the second reagent in the third chamber 20. In addition, there is a direct injection method, that is, when the second container 200 carries the separating component 13 together and cannot be pushed any more, the puncture device 21 pierces the separating component 13, so that the first reagent flows into the second reagent in the third cavity 20. This way, there is a large pressure difference across the partition 13 before the piercer 21 pierces the partition 13, and the volume in the first container 100 is compressed, resulting in an internal pressure that is much greater than the pressure in the second container 200, piercer 21. The instant when the piercer 21 pierces the partition 13, the pressure is released, causing the first reagent in the first container 100 to flow into the piercer 21 and the third cavity 20 at an uncontrollable high flow rate, which also causes a problem that the target amount of the first reagent added cannot be guaranteed.

In one embodiment, during the movement of the second container 200 to the side closer to the first container 100, the second container 200 has an initial position of docking with respect to the first container 100, as shown in fig. 2. When piercer 21 pierces first seal 22 and partition 13, second container 200 has a piercing position relative to first container 100, as shown in fig. 3. Prior to piercer 21 piercing first seal 22 and partition 13, partition 13 is stationary relative to first container 100; after the piercer 21 pierces the first seal 22 and the partition 13, the second container 200 moves the partition 13 a predetermined distance further to reach the end position, as shown in fig. 4. At this time, a predetermined amount of the first reagent is dropped into the third chamber 20 through the passage of the puncture instrument 21.

Wherein, the first distance is arranged between the initial position and the puncture position. The puncture location is spaced a second distance from the termination location. The second distance is determined according to the volume and the flow cross-sectional area of the first chamber 110, the volume and viscosity of the first reagent, the target amount of the first reagent required for the test, and the flow cross-sectional area of the puncture instrument 21. When the first chamber 110 is a cylindrical body, the flow cross-sectional area is proportional to the inner diameter. Similarly, when a cylindrical opening is formed in the puncture instrument 21, the flow cross-sectional area is proportional to the inner diameter thereof.

The first distance is primarily dependent on the height of the piercer 21 exposing the first seal 22. When the puncture body 21 is provided with a protective sheath 223 on top, the first distance depends on the height of the puncture instrument 21 exposing the first seal 22, superimposed on the thickness of the protective sheath 223.

In this embodiment, by properly setting the volume of the first cavity 110, the volume and viscosity of the first reagent, the cross-sectional flow area of the puncture device 21, and the target amount of the first reagent required for the test, the preset distance (the first distance from the initial position to the puncture position, and the second distance from the puncture position to the end position) that the second container 200 needs to move relative to the first container 100 at each stage can be determined, and according to the preset distance, the matching structure of the second container 200 and the second container 200 can be set, so that during the movement of the second container 200 near the first container 100 side, the second container 200 moves the first distance before the partition 13 punctures, and the partition 13 does not move, i.e., the volume of the first cavity 110 is not compressed; subsequently, after the puncture device 21 punctures the separating component 13 (i.e. the first cavity 110 is communicated with the third cavity 20, the initial pressure of the two is kept consistent), the second container 200 drives the separating component 13 to move a second distance, so as to accurately drop the target amount of the first reagent in the first cavity 110 into the third cavity 20 through the puncture device 21.

To verify the above theory, the inventors conducted several sets of comparative experiments.

Experiment one:

the inner diameter of the first container 100 is 11mm, the effective volume (volume) is about 3ml, the volume (volume) of the second container 200 is 0.5ml, the inner diameter of the puncture instrument 21 is 2mm at most, and the viscosity of the first reagent is the same as that of water.

When testing, firstly, 2ml of first reagent is added into the first container 100; the second container 200 is rotated to push the partition 13 to move about 7mm (about 0.6ml of the first container), so that about 130. mu.l (microliter) of the first reagent can be introduced into the second container 200. If the amount of the first reagent added is reduced to about 800. mu.l, the second container 200 is rotated to push the partition member 13 to move about 7mm (to squeeze a volume of about 0.6ml in the first container 100), and about 70. mu.l of the first reagent can be discharged into the second container 200.

It should be noted that in this process, the volume of air in the first container 100 is displaced during the process of rotating the second container to push the partition member 13 to move the second distance.

Experiment two:

the inner diameter of the first container 100 is 11mm, the effective volume is about 3ml, the volume of the second container 200 is 0.5ml, the inner diameter of the puncture outfit 21 is 2mm at most, and the viscosity of the first reagent is the same as that of water. Mu.l of the first reagent is added to the first container 100, and if necessary, 30. mu.l of the first reagent is introduced into the

second container

200, and 30. mu.l of the first reagent can be poured into the second container 200 by setting the height of the screw to be effective to push the partition member 13 to move about 2.5mm (to squeeze a volume of about 0.2ml in the first container 100).

In summary, by adjusting the amount of the first reagent added and pushing the divider 13 to occupy the volume of the first container 100, it is possible to achieve a precise derivation of the volume of the first reagent, given the basic parameters of the first container 100, the second container 200, the perforator 21 and the first reagent.

Specifically, under the premise that the volume parameters of the first container 100 and the second container 200 are determined, the diameter of the puncture 21 is determined, and the viscosity of the first reagent is determined, 700 μ l of the first reagent is added into the first container 100, and every time the partition 13 is pushed to occupy 0.2ml of the volume of the first container, 25 μ l of the first reagent can be led out into the second container 200.

According to the above relationship, if about 60. mu.l of the first reagent is required to be added, the first container is required to be squeezed to a volume of 0.6 ml. The thread height thus calculated ensures that the partition 13 can move and squeeze out 0.6ml of the volume of the first container 100. If 100. mu.l of the first reagent is to be removed, a volume of 0.8ml of the first container 100 is squeezed out; if the first reagent amount to be derived is about 45 μ l, a volume of 0.4ml of the first container 100 may be squeezed out.

Overall, the volume of first reagent to be derived is proportional to the (air) volume pushing the partition 13 against the first container 100, with the basic parameters (volume and cross-sectional flow area of the first container 100, volume and viscosity of the first reagent, cross-sectional flow area of the piercer 21) determined.

If the volume of the first reagent added to the first container 100 is adjusted, for example, not 700. mu.l in experiment two but 2ml in experiment one, then the same amount of 0.6ml is squeezed out, and the amount of liquid to be discharged is about 130. mu.l. Similarly, multiple sets of experiments may be performed, determining the volume of first reagent that is derived in proportion to the volume of (air) that pushes partition 13 against first container 100 in the case of experiment one.

Referring to fig. 5 to 6 or 9, in the present embodiment, a position-limiting portion 14 for limiting the position of the partition 13 or the first sealing element 22 along the axial direction of the cylinder is disposed on the inner wall of the first container 100.

The position-limiting part 14 is used for preliminarily limiting the partition 13 or the first sealing element 22, so that when the piercer 21 of the second container 200 pierces the first sealing element 22 and the partition 13 after the first container 100 is connected with the second container 200, the position-limiting part 14 can provide a certain axial abutting force to ensure that the piercer 21 can reliably pierce the first sealing element 22 and the partition 13. The divider 13 is stationary relative to the first container 100 before the first seal 22, divider 13 is pierced, and is able to move synchronously with the second container 200 after the first seal 22, divider 13 is pierced.

Specifically, the limiting portion 14 may be a ring-shaped or point-shaped protrusion structure disposed on the inner wall of the first container 100. Of course, the specific form of the limiting portion 14 may be other forms capable of achieving limiting, and the application is not limited specifically herein.

In particular, the position-limiting portion 14 may be located on an upper side (i.e., a side close to the second end 12 in the drawing) of the partition 13 so as to provide an axial abutting force for the partition 13. Further, the stopper portion 14 may be located at a lower side of the partition 13 so as to provide an axial abutting force for the first seal member 22.

In the present embodiment, the separator 13 may include any one of: a rubber plug and a piston.

When separator 13 is the form of plug, the plug is whole to be set up in the barrel through interference fit's mode, and the middle part of plug is provided with and remains to pierce through the portion. The limiting part 14 is located on one side of the rubber plug close to the first end. After the second container 200 moves a first distance near the limiting portion 14, the puncture outfit 21 punctures the portion to be punctured limited by the limiting portion, and under the action of the first acting force, the second container 200 can drive the rubber plug to move a second distance to the first end after passing through the limiting portion 14.

When the partition 13 is in the form of a piston, the piston comprises: a main body of the part to be pierced is arranged at the center of one end part, and a sealing ring sleeved on the outer wall of the main body. The sealing ring is in interference fit with the cylinder body. The limiting part 14 is positioned on one side of the piston near the second end. After the second container 200 moves a first distance to contact with the position-limiting portion 14, the puncture device 21 punctures the portion to be punctured, and under the action of the second acting force, the second container 200 can cross the position-limiting portion 14 to drive the piston to move a second distance to the first end.

Specific values of the first acting force and the second acting force may be determined according to the matching relationship between the partition 13 and the corresponding component, and the like, and the application is not limited specifically herein.

When the puncture device 21 punctures the portion to be punctured, the first cavity 110 and the second cavity 120 are communicated through the puncture device 21, and the initial pressures of the two are equal. The second distance for continuing the upward movement is positively correlated with the target amount of the first reagent required to be dropped. In general, the first container 100 may be a container with a circular cross section, and the inner diameter of the container is a known amount, so that the volume of the first reagent dropped into the third container is ensured to be a target amount after the second distance for further upward movement is determined.

In one embodiment, the first container 100 and the second container 200 may be connected by a screw thread. Specifically, a first external thread 15 is provided on the outer side wall of the cylinder body near the second end 12 of the first container 100, and a first internal thread 23 matched with the first external thread 15 is provided on the inner wall of the second container 200. In use, when the second container 200 is rotated in a first direction relative to the first container 100, the second container 200 can be moved closer to the first container 100.

For the second container, a first accommodating portion 201 for mounting the puncture outfit 21 and a second accommodating portion 202 for accommodating a second reagent are arranged in a third cavity of the second container, a third accommodating portion 203 is arranged on the periphery of the first accommodating portion 201, the first internal thread 23 is arranged on the third accommodating portion 203, and a clearance structure 24 is formed on one side of the third accommodating portion 203, which is far away from the first internal thread 23.

This keep away empty structure 24 can be for setting up the smooth inner wall in first internal thread 23 downside, through setting up this keep away empty structure 24, can reduce the preparation degree of difficulty of this third portion of accommodating 203 on the one hand, guarantees the successful drawing of patterns, and on the other hand is favorable to reducing the resistance that the pipe wall of first container 100 stretched into down.

The first, second, and third

accommodating portions

201, 202, and 203 may be formed integrally or may be formed partially or entirely separately. For example, as shown in fig. 7, the first, second, and

third accommodation portions

201, 202, and 203 may be an integral structure. As shown in fig. 12, the first container 201 and the third container 203 are integrally configured, and the first container 201 and the third container 203 for containing the second reagent are separately configured.

The container that this application embodiment provided can match the volume of the first reagent that needs to add according to the second reagent that detects or experiment set for, through the thread height of designing different size specifications, and the container of different specifications can be designed to the removal distance of separator 13 and puncture ware 21 diameter isoparametric to satisfy different test demands.

Referring to fig. 8, in one embodiment, the cover 30 is a hollow structure with two open ends, and has a bottom end relatively close to the second end and a top end relatively far away from the second end. The composite cover 300 further includes a second sealing member 31 disposed at the top end, and a snap member 32 press-fitted on the second sealing member 31.

The second sealing member 31 includes a base, a peripheral side portion disposed at the periphery of the base and used for forming a seal at the position where the cover 30 is engaged with the first container 100, and a convex portion disposed at one side of the base and used for forming a seal with the top end of the cover 30 and optionally with the first end 11 of the first container 100. The cover 30 is provided with an annular mounting groove for mounting the peripheral side portion, and the convex portion is in sealing engagement with the inner wall of the first container 100. Specifically, the second sealing member 31 may be made of a material having certain elasticity, which can be matched with the first container 100 by an interference fit.

In order to ensure the sealing performance of the position where the second sealing member 31 is engaged with the first container 100, the protruding portion may be a cylinder having an outer diameter equal to or slightly larger than the diameter of the first container 100, and at least one annular protrusion corresponding to a sealing ring may be provided on a sidewall of the cylinder. In order to better ensure the tightness of the second seal 31 in cooperation with the first container 100, the annular protrusions may be axially multiple, for example, two as shown in fig. 8.

In the present embodiment, in order to install the second sealing member 31 with a specific shape in the cover body 30 in a convenient manner and achieve sealing engagement with the first container 100, the top end of the cover body 30 is provided as an open end, when being installed, the second sealing member 31 can be installed into the cover body 30 from the open end to seal the cover body 30, and then can be selectively engaged with the upper end of the first container 100 to form sealing contact according to the use state.

In order to position the second seal 31, particularly when the second seal 31 seals the first container 100, an engaging piece 32 is further provided on the upper side of the second seal 31 (i.e., on the side away from the first container 100) in order to prevent the second seal 31 from being displaced. Specifically, the engaging piece 32 may be annular as a whole, and a first engaging portion 320 is provided on an outer side wall of the engaging piece 32. The cover 30 is provided with a first engaging groove 302 on an inner wall near the top end for engaging with the first engaging portion 320. The first engaging portion 320 cooperates with the first engaging groove 302 to form an engaging structure for fixing the second sealing member 31. The engaging member 32 and the cover 30 form the engaging structure, so that the second sealing member 31 is limited.

In this embodiment, the composite cover 300 is at least axially movable with respect to the first container 100 to switch the open or closed state of the window 301.

Specifically, the relative movement relationship between the combination cover 300 and the first container 100 can be set according to the specific matching structure of the two. For example, the combined cover 300 and the first container 100 may move only relatively, and in particular, the combined cover 300 may move axially relative to the first container 100. Alternatively, the combination cap 300 and the first container 100 may be moved and rotated in a combined manner, and specifically, the combination cap may be rotated first, then moved and then rotated with respect to the first container 100. In addition, the combination cap 300 can rotate relative to the first container 100, and particularly, during the rotation, the combination cap 300 can rotate axially relative to the first container 100, so that the position of the window 301 relative to the first container 100 is changed, and the opening or closing state of the window 301 is switched.

In one embodiment, a first engagement portion 16 is provided on the outer wall of the cylinder, and a second engagement portion 303 is provided on the inner wall of the cover 30. When the cover 30 is in the second position relative to the barrel, the first mating portion 16 and the second mating portion 303 cooperate to form a locking structure.

In the present embodiment, the composite cover 300 is axially movable with respect to the first container 100. By the locking mechanism formed by the first engaging portion 16 on the outer wall of the barrel and the second engaging portion 303 on the cover 30, when the cover 30 is located at the second position relative to the barrel, the cover 30 and the barrel are axially fixed, and the cover 30 is not easily moved relative to the first container 100 under the external force such as vibration, so as to automatically open the window 301.

Specifically, the first fitting portion 16 may include a plurality of second engaging grooves arranged at intervals around the circumferential direction of the cylinder. The second engaging portions 303 are a plurality of second engaging portions provided at intervals in the circumferential direction of the lid body 30. Alternatively, the first mating portion 16 may include a plurality of second locking grooves spaced apart along the axial direction of the barrel. The second engaging portions 303 are a plurality of second engaging portions provided at intervals in the circumferential direction of the lid body 30. The second locking groove and the second engaging portion may form a locking structure to prevent the combination cover 300 from being accidentally loosened when the combination cover 300 is located at the second position relative to the first container 100.

In one embodiment, in order to prevent the composite closure 300 from being separated from the first container 100 when being opened, a third engaging portion 17 is further provided on an outer wall of the cylinder near the first end 11. When the second engaging portion engages with the third engaging portion 17, the cover 30 is located at the farthest position away from the second end 12, and the window 301 is in the open state.

The second position may be a farthest position where the cover 30 can be away from the second end 12. Of course, the second position may also be lower than the most distant position.

As shown in fig. 5 to 6, in a specific embodiment, the first engaging portion 16 is a plurality of second engaging grooves spaced around the circumference of the cylinder, and the third engaging portion 17 is a protruding rib engaged with the second engaging grooves. When the cover body is located at the first position relative to the barrel body, the protruding ribs can be clamped into the second clamping grooves, so that the cover body 30 is limited, and the cover body is prevented from being accidentally loosened relative to the barrel body.

In another specific embodiment, as shown in fig. 9, the first fitting portion 16 includes: the two second clamping grooves are at least arranged along the axial direction of the barrel at intervals, and the through part is communicated with the two second clamping grooves; the third engaging portion 17 is two engaging pieces arranged at an interval. The through part can be a through groove formed on the outer surface of the cylinder and communicated with the upper second clamping groove and the lower second clamping groove. The through parts may be symmetrically installed on an outer wall of the middle part of the first cylinder.

When the cover body 30 is installed in cooperation with the cylinder body, the buckle of the cover body 30 can be installed through the clamping groove close to the upper end of the cylinder body; after the cover body 30 rotates a certain angle relative to the cylinder body, the buckle of the cover body 30 is matched with the through part; then, the cover 30 is axially moved relative to the cylinder until the cover 30 cannot be moved, and at this time, the cover 30 is rotated by a certain angle relative to the cylinder again, so that the fastener at the lower end of the cover 30 is clamped into the second clamping groove at the lower end of the cylinder, and the cover 30 and the cylinder are locked and matched with each other, at this time, the cover 30 cannot be axially opened, the window 301 is in a closed state, and the first cavity 110 is in a sealed state. When the cover body 30 is rotated reversely, the fastener can be disengaged from the second engaging groove and then pulled up to open the cover body 30.

Please refer to fig. 10, fig. 11 and fig. 12, and the present specification further provides another container, which has a similar main structure, composition, function and the like as those of the container provided in the above embodiment, and the difference between the two is mainly explained herein, and the same point can refer to the detailed description of the above embodiment, and the detailed description of the present application is not repeated herein.

In the present embodiment, the puncture instrument 21 is provided in the first container 100. Specifically, puncture ware 21 includes puncture portion 211 and is located the base 212 of puncture portion 211 one end, base 212 with the barrel is sealed to be cooperated, partition 13 cover is established outside puncture portion 211. As shown in fig. 13, the puncture instrument 21 may be a relatively hard injection-molded part. An annular groove may be provided on an outer sidewall of the base 212, and a sealing ring 214 may be provided therein. The sealing ring 214 may be made of rubber material to ensure the sealing property when the puncture device 21 is engaged with the inner wall of the barrel.

In the present embodiment, the separator 13 is hermetically covered outside the puncture instrument 21. Specifically, the partition 13 may include a first portion that is in sealing engagement with the inner sidewall of the first container 100 and a second portion that covers the piercing portion 211.

In this embodiment, the first sealing member 22 may be a sealing sheet provided at the open end of the second container 200. Wherein, the outer side wall of the second container 200 is provided with a second external thread 25, and the inner side wall of the first container 100 close to the first end 11 is provided with a second internal thread 18 matched with the second external thread 25. Wherein the second external thread 25 of the second container 200 has a start end and a finish end. Wherein the stop portion is formed by the cooperation between the terminal end and the outer wall of the second container 200.

When in specific use, the upper end of the second container 200 extends into the second end of the first container 100, and before the separating element 13 and the first sealing element 22 are punctured, the separating element 13 is limited by the limiting part 14 on the inner wall of the first container 100; when the partition 13 and the first sealing member 22 are pierced by the piercer 21, the second container 200 is further rotated relative to the first container 100, the second container 200 can drive the partition 13 and the piercer 21 to move towards the first end 12 of the first container 100, and when the second end 12 of the first container 100 is matched with the stop portion of the second container 200, the second container 200 is kept still relative to the first container 100, so that a certain amount of the first reagent in the first container 100 can be ensured to drop into the second container 200 through the passage of the piercer 21.

Based on the container provided in the above embodiments, the present application provides a corresponding method of use, which may include the following steps:

step S10: moving the cover 30 to a first position in a direction away from the second end 12 of the cylinder, opening the window 301, placing a sample containing a substance to be tested into the first container 100 through the window 301, moving the cover 30 to a second position close to the second end 12 of the cylinder, and closing the window 301; the sample is contacted with a first reagent contained in the first container 100;

step S12: when the second container 200 is mounted at the second end 12, the piercer 21 pierces the partition 13 and the first sealing member 22 to drip a predetermined volume of the first reagent into the second reagent in the second container 200 during the process of approaching the second container 200 to the first end 11.

Wherein the test sample comprises a main body part for bearing the measured object and an operation part connected with the main body part, and the method further comprises the following steps: at least part of the operating portion is broken when the window 301 is closed, preventing the object to be measured (sample) from being contaminated.

In a specific application scenario, when the container is used for extracting and detecting viruses, the main operation steps include two main types: packaging and formal use of the reagents before use.

When reagent packaging is carried out, the method comprises the following steps:

1.1, packaging a first reagent: in a corresponding clean production environment, the divider 13 is first loaded into the bottom of the first container 100, enclosing one end of the first container 100. The second sealing material 31 is fitted into the lid body 30 and fixed by the engaging member 32, thereby forming the composite lid 300. The first reagent is loaded into the first container 100 and is tightly covered by the combined cover 300, so that the snap at the bottom of the outer cover enters the corresponding clamping groove of the first container 100 and is locked, and the combined cover 300 is prevented from being opened before use.

1.2, packaging a second reagent: in a corresponding clean environment, the second reagent is filled into the second container 200, then is filled into the puncture outfit 21, and is tightly plugged and packaged by the first sealing member 22, so that the second reagent packaging is completed. The puncture instrument 21 may be protected by a protective sheath 223 before use.

In formal use:

2.1, the combined cover 300 can be opened, a tested object is placed in the window 301 of the cover body 30, and the redundant handheld part is broken off; the cap 30 and barrel are then locked to ensure that the cap cannot be opened. Typically, the length of the sample entering the cartridge is between 2 cm and 3 cm.

2.2, the first container 100 is sufficiently agitated to ensure that the analyte is sufficiently contacted with the first reagent.

And 2.3, connecting the second container 200 with the first container 100, pushing in and rotating until the second container rotates to the bottom, and determining that 2-3 drops of the first reagent are dripped into the second container 200.

And 2.4, putting the container into a tester, and operating the tester to read data.

In a specific embodiment, when the container provided by the present application is applied to virus detection, in a specific operation, the first reagent and the second reagent required by the virus detection may be packaged in 2 containers (the first container 100 and the second container 200), and a subsequent tester may complete the detection in a common environment through a simple operation. The specific operation steps are as follows:

the hand contact part of the measured object after the sample collection is finished is broken, and the sample entering the first container 100 is only kept 2-3 cm long, so that the sample is prevented from being polluted. After the sample enters the first container 100, the first container 100 is locked and is not opened any more, and the tester only needs to shake the first container 100 by hand to ensure that the sample is sufficiently soaked so as to be convenient for extracting nucleic acid. Then, the first container 100 and the second container 200 are coupled and rotated to the bottom. Thus, the puncture device 21 in the second container 200 can puncture the first container 100 and push the piston to move, so that the first reagent is dropped into 2-3 drops (designated dose) of the test solution. And then placing the container into a tester to test the read data.

On the whole, utilize the container that provides in this application embodiment to carry out needs biological test, chemical experiment etc. under airtight environment, for example, when virus draws and detects, solved the problem that the test must go on in biological laboratory, biological safety operation cabinet, reduced the security risk, improved detection efficiency, shorten detection latency greatly, reduce inspection cost.

It should be noted that, in the description of the present application, the terms "first", "second", and the like are used for descriptive purposes only and for distinguishing similar objects, and no precedence between the two is intended or should be construed to indicate or imply relative importance. In addition, in the description of the present application, "a plurality" means two or more unless otherwise specified.

The above embodiments in the present specification are all described in a progressive manner, and the same and similar parts among the embodiments may be referred to each other, and each embodiment is described with emphasis on being different from other embodiments.

The above description is only a few embodiments of the present invention, and although the embodiments of the present invention are described above, the above description is only for the convenience of understanding the present invention, and is not intended to limit the present invention. It will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention as defined by the appended claims.

Claims

1. A container, comprising:

the device comprises a first container, a second container and a third container, wherein the first container is integrally a hollow cylinder with two open ends, the cylinder is provided with a first end and a second end which are opposite to each other, a movable partition is arranged in the cylinder, the partition divides the cylinder into a first cavity and a second cavity, and a first reagent is contained in the first cavity;

a composite cover movably disposed at said first end, said composite cover comprising: the cover body is provided with a window, when the cover body is positioned at a first position relative to the cylinder body, the window is in an open state, when the cover body is positioned at a second position relative to the cylinder body, the window is in a closed state, and the combined cover seals the first end;

the second container is movably and hermetically arranged at the second end, a third cavity for containing a second reagent is formed in the second container, one side of the third cavity, which is far away from the second end, is a blocking end, and a first sealing element is arranged close to the second end;

and a puncture device disposed in the first container or the second container, wherein at least one through passage is formed in the puncture device, the puncture device can puncture the partition and the first sealing member during the process that the second container moves toward the first container, and the first reagent in the first cavity can flow into the third cavity through the passage.

2. The container of claim 1, wherein during movement of the second container toward the side of the first container, the second container has an initial position of abutment with respect to the first container, the second container has a piercing position with respect to the first container when the piercer pierces the first seal and the divider, and the divider is stationary with respect to the first container prior to the piercer piercing the first seal and the divider; after the puncture outfit punctures the first sealing element and the separating element, the second container drives the separating element to move continuously for a preset distance to reach a termination position, and a preset amount of the first reagent drops into the third cavity through the channel.

3. The container according to claim 2, wherein a stopper for stopping the partition or the first sealing member in the axial direction of the cylinder is provided on an inner wall of the first container.

4. The container of claim 3, wherein the spike is disposed in the second container, and the first seal is provided with a crown for sealing a side of the second cavity adjacent the second end, and a plug extending into the third cavity and sealing a position where the spike engages the third cavity.

5. The container of claim 4, wherein a first external thread is provided on the outside wall of the barrel near the second end and a first internal thread matching the first external thread is provided on the inside wall of the second container.

6. The container as claimed in claim 5, wherein a first accommodating portion for mounting the puncture outfit and a second accommodating portion for accommodating a second reagent are arranged in the third cavity, a third accommodating portion is arranged on the periphery of the first accommodating portion, the first internal thread is arranged on the third accommodating portion, and a clearance structure is formed on one side of the third accommodating portion, which is far away from the first internal thread.

7. The container of claim 6, wherein said first seal further comprises a protective sheath attached to said crown portion, said protective sheath fitting over said piercing portion.

8. The container of claim 1, wherein the lid body is a hollow structure with two open ends, and has a bottom end relatively close to the second end and a top end relatively far from the second end, and the combined lid further comprises a second sealing member disposed at the top end, and a snap-fit member press-fitted on the second sealing member.

9. The container according to claim 8, wherein the engaging member is annular, a first engaging portion is disposed on an outer side wall of the engaging member, a first engaging groove is disposed on an inner wall of the cover body near the top end, the first engaging portion and the first engaging groove cooperate to form an engaging structure for fixing the second sealing member.

10. The container of claim 1, wherein the composite cover is at least axially movable relative to the first container to switch the open or closed state of the window.

11. The container of claim 10, wherein the outer wall of the barrel is provided with a first mating portion and the inner wall of the cover is provided with a second mating portion, the first mating portion and the second mating portion cooperating to form a locking structure when the cover is in a second position relative to the barrel.

12. The container of claim 11, wherein the first engaging portion includes a plurality of second engaging grooves spaced around a circumferential direction of the barrel or along an axial direction of the barrel, and the second engaging portion is a plurality of second engaging portions spaced around a circumferential direction of the cap.

13. The container of claim 12, wherein a third engaging portion is further disposed on an outer wall of the barrel near the first end, when the second engaging portion engages with the third engaging portion, the cover is located at a farthest position away from the second end, and the window is in an open state.

14. The container of claim 13, wherein the first engaging portion is a plurality of second engaging grooves spaced around the circumference of the barrel, and the third engaging portion is an annular protruding rib.

15. The container of claim 13, wherein the first mating portion comprises: the two second clamping grooves are at least arranged along the axial direction of the barrel at intervals, and the through part is communicated with the two second clamping grooves; the third clamping part is two buckles arranged at intervals.

16. The container according to claim 4, wherein the partition 13 comprises any one of: a rubber plug and a piston.

17. The container according to claim 16, wherein when the separating member is a rubber plug, the rubber plug is integrally disposed in the barrel body in an interference fit manner, and a portion to be punctured is disposed in the middle of the rubber plug; the limiting part is positioned on one side of the rubber plug close to the first end, after the second container moves for a first distance close to the limiting part, the puncture outfit punctures the part to be punctured limited by the limiting part, and then the second container can drive the rubber plug to move for a second distance to the first end after crossing the limiting part; alternatively, the first and second electrodes may be,

when the partition is a piston, the piston includes: a tip center is provided with the main part of portion of waiting to pierce through to and the cover is established sealing ring on the main part outer wall, the sealing ring with barrel interference fit, spacing position in the piston is close to one side of second end, the second container remove first distance extremely with after spacing portion contacts, the puncture ware pierces through wait to pierce through the portion, the second container can cross spacing portion drives the piston again to first end removes the second distance.

18. The container of claim 3, wherein said spike is disposed in said first container, said spike including a spike portion and a base at one end of said spike portion, said base being in sealing engagement with said barrel, said partition fitting over said spike portion.

19. The container of claim 18, wherein the outer sidewall of the second container is provided with a second external thread, and the inner sidewall of the first container near the first end is provided with a second internal thread matching the second external thread.

20. A method of using the container of claim 1, comprising:

21. The method of using the container of claim 20 wherein during the movement of the second container toward the side of the first container, the second container has an initial position of abutment with respect to the first container, the second container has a piercing position with respect to the first container when the piercer pierces the first seal and the divider, and the divider is stationary with respect to the first container prior to the piercer piercing the divider and the first seal; after the puncture outfit punctures the first sealing element and the separating element, the second container drives the separating element to move for a preset distance to reach a termination position, and a preset amount of the first reagent is dripped into the third cavity through the channel.

22. The method of using a container of claim 21, wherein said piercing location is spaced a second distance from said terminal location, said second distance being determined based on the volume and cross-sectional flow area of said first cavity, the volume and viscosity of said first reagent, the target amount of said first reagent required for testing, and the cross-sectional flow area of said piercer.