CN110283706A - Spiral reaction tube - Google Patents
Spiral reaction tube Download PDFInfo
- Publication number
- CN110283706A CN110283706A CN201910660123.0A CN201910660123A CN110283706A CN 110283706 A CN110283706 A CN 110283706A CN 201910660123 A CN201910660123 A CN 201910660123A CN 110283706 A CN110283706 A CN 110283706A
- Authority
- CN
- China
- Prior art keywords
- upper cover
- screw thread
- tube body
- section
- linkage section
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
- 238000006243 chemical reaction Methods 0.000 title claims abstract description 26
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 39
- 239000012528 membrane Substances 0.000 claims abstract description 15
- 239000007788 liquid Substances 0.000 claims abstract description 14
- 239000007787 solid Substances 0.000 claims abstract description 14
- 210000005239 tubule Anatomy 0.000 claims description 9
- 238000011017 operating method Methods 0.000 abstract description 3
- 230000003321 amplification Effects 0.000 description 5
- 238000003199 nucleic acid amplification method Methods 0.000 description 5
- 230000000694 effects Effects 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 239000003795 chemical substances by application Substances 0.000 description 2
- 238000011109 contamination Methods 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 238000003752 polymerase chain reaction Methods 0.000 description 2
- 238000007789 sealing Methods 0.000 description 2
- 230000004544 DNA amplification Effects 0.000 description 1
- 238000012408 PCR amplification Methods 0.000 description 1
- 239000012807 PCR reagent Substances 0.000 description 1
- 239000005030 aluminium foil Substances 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 238000010367 cloning Methods 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 239000003085 diluting agent Substances 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 238000013467 fragmentation Methods 0.000 description 1
- 238000006062 fragmentation reaction Methods 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/14—Process control and prevention of errors
- B01L2200/141—Preventing contamination, tampering
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/04—Closures and closing means
- B01L2300/041—Connecting closures to device or container
- B01L2300/044—Connecting closures to device or container pierceable, e.g. films, membranes
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Hematology (AREA)
- Clinical Laboratory Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a kind of spiral reaction tube, including tube body, upper cover and connector sleeve, it is provided with limiting stand in connector sleeve, connector sleeve is divided into upper and lower linkage section by the diaphragm on limiting stand, is packaged with solid reagent in the seal chamber that upper cover and upper linkage section surround;Cutting membrane knife and press mold item are arranged at intervals in upper cover, the cutting membrane knife bottom for extending upper cover is helical teeth, and the sharp bottom of helical teeth is lower than press mold item;Upper linkage section is provided with screw thread platform, and the sharp bottom of helical teeth and diaphragm gap are arranged when the seal stopper ring of upper cover is screwed to screw thread platform;Tube body is threadedly coupled with lower linkage section, is packaged with liquid reagent in the seal chamber of tube body and lower linkage section.The present invention is completely separated by upper cover and tube body using diaphragm, and it is packaged with solid reagent, liquid reagent respectively, only diaphragm need to be punctured to the mixing that solid reagent and liquid reagent can be realized using the helical teeth of cutting membrane knife when use, without artificial repeatedly addition reagent, operating procedure is simplified, is improved work efficiency.
Description
Technical field
The present invention relates to PCR amplification containers, more particularly, to a kind of spiral reaction tube.
Background technique
Polymerase chain reaction (Polymerase Chain Reaction, i.e. PCR) is a kind of specific for amplifying amplification
DNA fragmentation Protocols in Molecular Biology, the features such as with high specific, high sensitivity and high efficiency, led in life science
Domain produces tremendous influence.PCR reaction tube is a kind of reaction vessel that PCR is generally used, by PCR thermal cycler to PCR
The amplification in vitro of DNA is realized in heating and cooling repeatedly for reaction solution in reaction tube.PCR reaction tube includes tube body and Guan Gai, such as
CN106906130A discloses the flat PCR reaction tube of high light transmission, CN201981185U discloses a kind of high-speed PCR reaction tube.But
It is that existing PCR reaction tube has the following problems: since DNA amplification reaction agents useful for same is at least 5 kinds, is needed before DNA cloning
Sample and plurality of reagents are successively manually precisely added in PCR reaction tube, time-consuming and cumbersome, and manages intracorporal reagent
Exposure duration is long, increases the contaminated probability of sample in tube body.Thus, PCR reagent step can be simplified by how designing one kind
PCR reaction tube be those skilled in the art's critical issue urgently to be resolved.
Summary of the invention
It is an object of that present invention to provide a kind of simplified amplification single reagents to add step, high-efficient and small contamination probability spiral
Formula reaction tube.
To achieve the above object, the present invention takes following technical proposals:
Spiral reaction tube of the present invention, including tube body and upper cover, the tube body and upper cover are linked together by connector sleeve,
It is provided with the limiting stand of structure in a ring in the connector sleeve, is provided with diaphragm on the limiting stand, the diaphragm is by connector sleeve
Be divided into linkage section and lower linkage section, the upper cover is threadedly coupled with upper linkage section, offered on upper linkage section inner sidewall with it is upper
Solid reagent is packaged in the seal chamber that the first internal screw thread that lid external thread section matches, upper cover and upper linkage section surround;It is described
The cutting membrane knife and press mold item extended vertically downwards is arranged at intervals in upper cover, the cutting membrane knife, press mold item extend downwardly out
The surface for covering and being respectively positioned on the diaphragm edge portion, the cutting membrane knife bottom for extending upper cover is helical teeth, the sharp bottom of the helical teeth
Lower than the press mold item;Upper linkage section inner sidewall between first internal screw thread and the limiting stand is provided with wedge-shaped knot
The screw thread platform of structure, the bottom of upper cover lateral wall are provided with the seal stopper ring matched with the screw thread platform, the seal stopper ring rotation
It is screwed at screw thread platform the sharp bottom for making helical teeth and diaphragm gap is arranged;The tube body is threadedly coupled with the lower linkage section, lower connection
The second internal screw thread matched with the tube body external thread section is offered in section, is packaged in tube body and the seal chamber of lower linkage section
Liquid reagent.
The tube body includes wide opening section and tubule section, and the wide opening section and tubule section are linked together by rotary table section.
The sharp bottom of the helical teeth and the difference in height of press mold bottom are less than the spacing of the screw thread platform and limiting stand, screw thread platform
It is less than the externally threaded screw pitch of the upper cover with the spacing of limiting stand.
The invention has the advantages that overcome normal PCR reaction tube there are contamination probabilities due to needing repeatedly addition reagent it is high,
The technical issues of time-consuming.Advantages of the present invention is embodied in the following:
(1), the present invention will using diaphragm upper cover and tube body is completely separated, be packaged with respectively in upper cover and tube body solid reagent,
Diaphragm need to only be punctured when use using the helical teeth of cutting membrane knife and the mixed of solid reagent and liquid reagent can be realized by liquid reagent
It closes, without artificial repeatedly addition amplifing reagent, simplifies operating procedure, shorten the reagent addition time, improve work efficiency,
The reagent exposed time is also greatly shortened, pollution probability is reduced.
(2), shipment state of the invention is that the seal stopper ring of upper cover is screwed to screw thread platform, can not only guarantee leakproofness
Can, also there is reminding effect, operator's upper cover and tube body is reminded to be in a sealed state.
(3), cutting membrane knife bottom of the invention is helical teeth, and helical teeth point bottom and the difference in height of press mold item are less than screw thread platform and limit
The spacing of the spacing of platform, screw thread platform and limiting stand is less than the externally threaded screw pitch of upper cover, so that rotation when upper cover is rotated to limiting stand
Gyration can not only guarantee that the solid reagent in upper cover can smoothly enter into tube body less than 360 °, moreover it is possible to guarantee diaphragm still with limit
Platform connection, avoids diaphragm from falling in the tube body of lower section and influence expanding effect.
(4) tube body of the invention is made of wide opening section, rotary table section and tubule section, and the wide opening section at top can guarantee that solid tries
Agent smoothly falls to rotary table section or tubule section, and tubule section increases the contact area of sample and reagent, improves sample and reagent
Reaction rate.
Detailed description of the invention
Fig. 1 is structural schematic diagram of the invention.
Fig. 2 is usage state diagram of the invention.
Specific embodiment
As shown in Figure 1, spiral reaction tube of the present invention, including tube body and upper cover 1, the tube body and upper cover 1 are logical
It crosses connector sleeve to be linked together, the limiting stand 2.1 of structure in a ring is provided in the connector sleeve, is arranged on the limiting stand 2.1
There are diaphragm 2.2 made of aluminium-foil paper, connector sleeve is divided into upper linkage section 2.3 and lower linkage section 2.4, upper cover 1 by the diaphragm 2.2
It is threadedly coupled, is offered on 2.3 inner sidewall of upper linkage section above screw thread platform 2.6 and the outer spiral shell of upper cover 1 with upper linkage section 2.3
Solid reagent 8 is packaged in the seal chamber that the first internal screw thread 2.5 that line section matches, upper cover 1 and upper linkage section 2.3 surround;On
The cutting membrane knife 3 and press mold item 4 extended vertically downwards is arranged at intervals in lid 1, the cutting membrane knife 3, press mold item 4 extend downwardly out
Upper cover 1 and the surface for being respectively positioned on 2.2 edge part of diaphragm, 3 bottom of cutting membrane knife for extending upper cover 1 is helical teeth 5, helical teeth 5
Sharp bottom be located at the inside of press mold item 4, the sharp bottom of helical teeth 5 is lower than the press mold item 4, the sharp bottom of helical teeth 5 and the height of 4 bottom of press mold item
Degree difference is less than the screw pitch of first internal screw thread 2.5;It is upper between first internal screw thread 2.5 and the limiting stand 2.1
2.3 inner sidewall of linkage section is provided with the screw thread platform 2.6 of wedge structure, and the bottom of 1 lateral wall of upper cover is provided with and 2.6 phase of screw thread platform
The seal stopper ring 6 of cooperation, seal stopper ring 6 are screwed to the sharp bottom and 2.2 gap setting of diaphragm for making helical teeth 5 at screw thread platform 2.6, are avoided
Diaphragm 2.2 is punctured and solid reagent and liquid reagent is caused to mix by helical teeth 5 when non-use;Tube body and the lower linkage section 2.4
It is threadedly coupled, the second internal screw thread 2.7 matched with the tube body external thread section is offered in lower linkage section 2.4, tube body is under
Liquid reagent is packaged in the seal chamber of linkage section 2.4, liquid reagent is the mixed liquor of plurality of liquid reagent.
When actual processing, there are gap, the sharp bottom of helical teeth 5 and the height of 4 bottom of press mold item for the sharp bottom of helical teeth 5 and diaphragm 2.2
Degree difference is less than the spacing of screw thread platform 2.6 and limiting stand 2.1, and screw thread platform 2.6 and the spacing of limiting stand 2.1 are less than 1 external screw thread of upper cover
Screw pitch so that using when upper cover 1 rotation angle can be rotated in place (when i.e. upper cover 1 is screwed to screw thread platform less than 360 °
Rotation angle less than 360 °), and then by the helical teeth of cutting membrane knife 35 from contact diaphragm 2.2 to the rotation angle control cut after film
System is at 240 °~355 °, it is ensured that upper cover 1 screws metacneme 2.2 in place and connect with 2.1 part of limiting stand, it is ensured that solid reagent is smooth
Into in tube body, diaphragm 2.2 is avoided to fall in the tube body of lower section and influence expanding effect.
As shown in Figure 1, the tube body includes wide opening section 7.1 and tubule section 7.2, the wide opening section 7.1 and tubule section 7.2 are logical
It crosses rotary table section 7.3 to be linked together, wide opening section 7.1 ensures that solid reagent can smoothly enter into tube body, and the top surface of wide opening section 7.1 is to compress
Face, seal face and 2.1 lower surface of limiting stand are tightly connected, and guarantee the sealing performance of tube body;Tubule section 7.2 increases sample and reagent
Contact area, improve the reaction rate of sample and reagent.
Specifically used process description of the invention is as follows:
When needing to carry out amplification reaction sample, operator takes out this spiral reaction tube, using external impetus or manually
It screws tube body and screws from connector sleeve by it, sample is then added into tube body (if the liquid reagent encapsulated in tube body
For concentrated reagent, it is also necessary to add dilution into tube body), tube body is screwed again after sample is sufficiently mixed with liquid reagent
On connector sleeve, upper cover 1 then is screwed using external impetus or manually, and upper cover 1 screws in downwards since the screw thread platform 2.6, cuts film
Diaphragm 2.2 is cut while the sharp bottom of helical teeth 5 circles in 3 rotary course of knife, is pressed when upper cover rotates 300 °~350 °
Film item 4 starts press mold when dropping at diaphragm 2.2, stops when seal stopper ring 6 is screwed to the sealing cooperation of 2.1 upper surface of limiting stand
Film is only cut, the diaphragm 2.2 for cutting 4 corresponding position of press mold item after the completion of film is still connect with limiting stand 2.1, is avoided diaphragm from falling off, is made simultaneously
Solid reagent in upper cover 1 smoothly out of, limiting stand 2.1 falls to lower section tube body, carries out anti-with the intracorporal mixing liquid of pipe
It answers, whole operation process only needs to add sample and diluent manually, simplifies operating procedure, shortens the operating time, improves
Working efficiency, while the exposed time of tube body is also substantially reduced, the contaminated probability of sample is thereby reduced, guarantees that amplification is anti-
Answer the reliability of result.
Claims (3)
1. a kind of spiral reaction tube, including tube body and upper cover (1), it is characterised in that: the tube body and upper cover (1) pass through connection
Set is linked together, and the limiting stand (2.1) of structure in a ring is provided in the connector sleeve, is provided on the limiting stand (2.1)
Connector sleeve is divided into upper linkage section (2.3) and lower linkage section (2.4) by diaphragm (2.2), the diaphragm (2.2), upper cover (1) with it is described
Upper linkage section (2.3) is threadedly coupled, and the matched with upper cover (1) external thread section is offered on upper linkage section (2.3) inner sidewall
One internal screw thread (2.5) is packaged with solid reagent in the seal chamber that upper cover (1) and upper linkage section (2.3) surround;The upper cover (1)
It is inside arranged at intervals with the cutting membrane knife (3) and press mold item (4) extended vertically downwards, the cutting membrane knife (3), press mold item (4) are downwards
Extend upper cover (1) and be respectively positioned on the surface of the diaphragm (2.2) edge part, extends cutting membrane knife (3) bottom of upper cover (1)
Sharp bottom for helical teeth (5), the helical teeth (5) is lower than the press mold item (4);Positioned at first internal screw thread (2.5) and the limit
Upper linkage section (2.3) inner sidewall between position platform (2.1) is provided with the screw thread platform (2.6) of wedge structure, upper cover (1) lateral wall
Bottom is provided with the seal stopper ring (6) matched with the screw thread platform (2.6), and the seal stopper ring (6) screws to screw thread platform
(2.6) make sharp bottom and diaphragm (2.2) gap setting of helical teeth (5) at;The tube body and lower linkage section (2.4) screw thread connect
It connects, the second internal screw thread (2.7) matched with the tube body external thread section, tube body and lower company is offered in lower linkage section (2.4)
It connects in the seal chamber of section (2.4) and is packaged with liquid reagent.
2. spiral reaction tube according to claim 1, it is characterised in that: the tube body includes wide opening section (7.1) and thin
Pipeline section (7.2), the wide opening section (7.1) and tubule section (7.2) are linked together by rotary table section (7.3).
3. spiral reaction tube according to claim 1 or 2, it is characterised in that: the sharp bottom of the helical teeth (5) and press mold item
(4) difference in height of bottom is less than the spacing of the screw thread platform (2.6) and limiting stand (2.1), and the spacing of screw thread platform and limiting stand is small
In the upper cover (1) externally threaded screw pitch.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201910660123.0A CN110283706A (en) | 2019-07-22 | 2019-07-22 | Spiral reaction tube |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201910660123.0A CN110283706A (en) | 2019-07-22 | 2019-07-22 | Spiral reaction tube |
Publications (1)
Publication Number | Publication Date |
---|---|
CN110283706A true CN110283706A (en) | 2019-09-27 |
Family
ID=68023726
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201910660123.0A Withdrawn CN110283706A (en) | 2019-07-22 | 2019-07-22 | Spiral reaction tube |
Country Status (1)
Country | Link |
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CN (1) | CN110283706A (en) |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2022041402A1 (en) * | 2020-08-24 | 2022-03-03 | Renolit Hansen Packaging Tech. (Bj) Ltd. | Container and method of using the same |
CN115678751A (en) * | 2022-12-30 | 2023-02-03 | 北京金诺美科技股份有限公司 | Sample tube |
Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP3157911U (en) * | 2009-12-22 | 2010-03-04 | 景岳生物科技股▲ふん▼有限公司 | Bottle lid device |
US20130058595A1 (en) * | 2010-05-04 | 2013-03-07 | Robert Bosch Gmbh | Screw closure for soft packaging |
KR20150075818A (en) * | 2013-12-26 | 2015-07-06 | (주)삼화피앤티 | The cap with container of additives |
CN106289933A (en) * | 2015-05-27 | 2017-01-04 | 艾博生物医药(杭州)有限公司 | A kind of lid and storage also mix the device of solid-liquid |
CN108531377A (en) * | 2018-06-26 | 2018-09-14 | 广东省第二人民医院(广东省卫生应急医院) | It is a kind of can pre-filled reagent diaphragm type nucleic acid amplification airtight reactor tube |
CN210367679U (en) * | 2019-07-22 | 2020-04-21 | 安图实验仪器(郑州)有限公司 | Spiral reaction tube |
-
2019
- 2019-07-22 CN CN201910660123.0A patent/CN110283706A/en not_active Withdrawn
Patent Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP3157911U (en) * | 2009-12-22 | 2010-03-04 | 景岳生物科技股▲ふん▼有限公司 | Bottle lid device |
US20130058595A1 (en) * | 2010-05-04 | 2013-03-07 | Robert Bosch Gmbh | Screw closure for soft packaging |
KR20150075818A (en) * | 2013-12-26 | 2015-07-06 | (주)삼화피앤티 | The cap with container of additives |
CN106289933A (en) * | 2015-05-27 | 2017-01-04 | 艾博生物医药(杭州)有限公司 | A kind of lid and storage also mix the device of solid-liquid |
CN108531377A (en) * | 2018-06-26 | 2018-09-14 | 广东省第二人民医院(广东省卫生应急医院) | It is a kind of can pre-filled reagent diaphragm type nucleic acid amplification airtight reactor tube |
CN210367679U (en) * | 2019-07-22 | 2020-04-21 | 安图实验仪器(郑州)有限公司 | Spiral reaction tube |
Non-Patent Citations (2)
Title |
---|
JIANPING WANG 等: "Closed-Tube PCR with Nested Serial Invasion Probe Visualization Using Gold Nanoparticles", CLINICAL CHEMISTRY, vol. 63, no. 4, 31 December 2017 (2017-12-31), pages 852 * |
姚梅宏 等: "不同荧光定量PCR 试剂盒在结核分枝杆菌检测中的应用", 临床与实验病理学杂志, vol. 34, no. 12, 27 December 2018 (2018-12-27), pages 1392 - 1394 * |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2022041402A1 (en) * | 2020-08-24 | 2022-03-03 | Renolit Hansen Packaging Tech. (Bj) Ltd. | Container and method of using the same |
CN115678751A (en) * | 2022-12-30 | 2023-02-03 | 北京金诺美科技股份有限公司 | Sample tube |
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PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
CB02 | Change of applicant information | ||
CB02 | Change of applicant information |
Country or region after: China Address after: 450016 No.199, Jingkai 15th Street, Zhengzhou Economic and Technological Development Zone, Henan Province Applicant after: AUTOBIO LABTEC INSTRUMENTS Co.,Ltd. Address before: No. 199, 15th Street, economic and Technological Development Zone, Zhengzhou City, Henan Province Applicant before: AUTOBIO LABTEC INSTRUMENTS Co.,Ltd. Country or region before: China |
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Application publication date: 20190927 |