CN215162693U - Integrated test paper strip type sampling amplification tube - Google Patents
Integrated test paper strip type sampling amplification tube Download PDFInfo
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- CN215162693U CN215162693U CN202121323590.3U CN202121323590U CN215162693U CN 215162693 U CN215162693 U CN 215162693U CN 202121323590 U CN202121323590 U CN 202121323590U CN 215162693 U CN215162693 U CN 215162693U
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- basement membrane
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Abstract
The utility model provides a strip-type sampling amplification pipe of integration test paper relates to strip-type sampling amplification pipe technical field of test paper. Inside being close to base department of pipe shaft is equipped with semicircular wall, circular shape basement membrane and circular shape bottom plate, be the second district between wall and the basement membrane, be the third district between basement membrane and the bottom plate, cut off the radius of the circular profile in place, the radius of basement membrane all equals with the inside radius of pipe shaft, be equipped with the test paper strip storehouse on the pipe shaft, communicate through the preformed hole between third district and the test paper strip storehouse, preformed hole department is equipped with the paraffin shutoff, there is preserving fluid in first district and the second district, there is detection liquid in the third district, the test paper strip storehouse embeds there is the test paper strip, it is equipped with the point prick to impel body one side near the pipe shaft, the locking circle is established in the outside of impelling the body, the external diameter of locking circle is greater than the internal diameter of pipe shaft. The utility model discloses a virus nucleic acid integration detection device integrates sampling, amplification and detection, can realize the on-the-spot quick check, has easy and simple to handle, sensitivity is high, the short advantage of check-out time.
Description
Technical Field
The utility model relates to a strip-shaped sampling amplification pipe technical field of test paper, in particular to strip-shaped sampling amplification pipe of integration test paper.
Background
Due to the fact that the virus load in cold-chain fresh food or related environment is low, the conventional sampling and detecting method has the risk of missing detection, and the conventional nucleic acid detecting device has the defects of high requirement on environment, long detecting time, high operation difficulty, difficulty in realizing on-site quick detection and the like.
However, China needs to import a large amount of cold-chain commodities and raw materials every day, and for institutional departments such as import and export ports, food production enterprises and the like, an integrated virus nucleic acid detection device which is simple and convenient to operate, high in sensitivity, short in detection time and capable of achieving on-site quick detection is urgently needed to be developed, and the aim of preventing and controlling epidemic situations on the forefront line and killing infection hidden dangers in the bud stage is achieved.
SUMMERY OF THE UTILITY MODEL
The utility model provides an integration test paper strip type sampling amplification pipe overcomes the defect that above-mentioned prior art exists.
The specific technical scheme is that the integrated test paper strip-shaped sampling amplification tube comprises: upper cover, pipe shaft and base, pipe shaft one end is installed on the base, the other end with upper cover threaded connection, inside being close to of pipe shaft base department is equipped with semicircular wall, circular shape basement membrane and circular shape bottom plate, inside with the wall and the basement membrane divide into first district, second district and third district, the bottom plate is located the tip of pipe shaft, the wall with be the second district between the basement membrane, be the third district between basement membrane and the bottom plate, the radius of cutting off place circular profile, the radius of basement membrane all equals with the inside radius of pipe shaft, be equipped with the test paper strip storehouse on the pipe shaft, the third district with through the preformed hole intercommunication between the test paper strip storehouse, preformed hole department is equipped with the paraffin shutoff, there is detection liquid in first district and the second district, there is detection liquid in the third district, the test strip storehouse embeds there is the test strip, the base includes: the pipe body is provided with a sharp prick cone at one side, the propelling body is close to the pipe body, the locking ring is arranged at the outer side of the propelling body, and the outer diameter of the locking ring is larger than the inner diameter of the pipe body.
Furthermore, a guide hole for the sharp pricker to penetrate through is formed in the bottom plate, the guide hole is close to the inner wall of the tube body, and the projection of the guide hole, which is parallel to the axis of the tube body, falls on the partition.
Further, the base further includes: one end of the connecting piece is connected with the tube body, the propelling body is arranged at one end of the connecting piece, and the outer diameter of the propelling body is larger than the inner diameter of the tube body and smaller than the inner diameter of the connecting piece.
Furthermore, the locking ring is rigidly connected with the convex locking nose at the opening of the ring.
Further, a supporting seat is arranged on the tube body, and the supporting seat is arranged on the same side of the test strip bin.
Before use, the sampling amplification tube is vertically placed; when the sampling amplification tube is used, the upper cover is unscrewed, a sampling cotton swab can be stretched into the preservation solution in the tube body to be fully contacted, elution and sample retention are completed, the upper cover is screwed, the preservation solution is shaken uniformly, the sampling amplification tube is transversely arranged, the gap side of the partition is arranged at the upper side, the liquid level in the second area is kept equal to the height of the partition through the interception effect of the partition, the liquid volume in the second area is ensured to be fixed, the locking ring is torn off during detection, the sharp-point cone is screwed to the lower side, the propelling body is propelled, the sharp-point cone pierces a sharp-point hole on the bottom membrane, the propelling body is withdrawn in the opposite direction, the sampling amplification tube is transversely arranged in the oven, the liquid in the second area flows into the third area along the sharp-point hole to be mixed with the detection solution, an amplification reaction is carried out at 60 ℃, the reaction is completed, the paraffin wax is heated to 80 ℃, and the paraffin wax at the reserved hole is sealed, the preformed hole is opened, reaction liquid in the third zone can flow into the test strip storehouse with the test strip meets, can appear changing on the test strip, generally can carry out the judgement directly perceived according to the colour change.
Compared with the prior art, the utility model discloses there is following technical advantage: the utility model is a virus nucleic acid integrated detection device, which integrates sampling, amplification and detection, can realize on-site quick detection, and has the advantages of simple operation, high sensitivity and short detection time; the arrangement of the guide hole limits the propelling position of the sharp prick cone, and the propelling body is propelled only by aligning the sharp prick cone with the guide hole, so that the misoperation that the sharp prick cone penetrates above the bottom film and cannot be detected is avoided, and the detection operation accuracy is improved; the length design of connecting piece needs to satisfy the point prick awl pierces the basement membrane does not touch simultaneously the propulsion stroke requirement of wall, the setting of connecting piece is convenient for control the stroke of propulsion body improves the operation standardization.
Drawings
The accompanying drawings, which are incorporated in and constitute a part of this application, are included to provide a further understanding of the invention, and are incorporated in and constitute a part of this specification. In the drawings:
FIG. 1 is a schematic vertical view of an integrated test strip-type sampling amplification tube;
FIG. 2 is a cross-sectional view taken at A-A of FIG. 1;
FIG. 3 is another cross-sectional view taken at A-A of FIG. 1;
FIG. 4 is a schematic view of an integrated test strip-shaped sampling amplification tube in a horizontal arrangement;
FIG. 5 is a sectional view of the tube body after use in example 2.
Wherein:
1. the test strip comprises an upper cover, 2, a tube body, 3, a connecting piece, 4, a propelling body, 5, a locking ring, 201, a partition, 202, a bottom film, 203, a bottom plate, 204, a first area, 205, a second area, 206, a third area, 207, a test strip bin, 208, a test strip, 209, a guide hole, 210, a preformed hole, 211, a sharp thorn hole, 212, paraffin plugging, 213, a supporting seat, 401, a sharp thorn cone, 501 and a locking nose.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention will be described in further detail with reference to the following embodiments and accompanying drawings. The exemplary embodiments and descriptions of the present invention are provided to explain the present invention, but not to limit the present invention.
In the description of the present invention, it should be noted that the terms "inner", "outer", "left" and "right" are used for indicating the position or positional relationship based on the positional relationship shown in the drawings, and are only for convenience of description and simplification of description, but do not indicate or imply that the device or element referred to must have a specific orientation, be constructed in a specific orientation and be operated, and thus should not be construed as limiting the present invention.
As will be appreciated in conjunction with figures 1-5,
example 1: referring to fig. 1 and 2, the integrated test strip type sampling amplification tube includes: upper cover 1, pipe shaft 2 and base, 2 one end of pipe shaft is installed on the base, the other end with 1 threaded connection of upper cover, 2 inside being close to of pipe shaft base department is equipped with semicircular wall 201, circular shape basement membrane 202 and circular shape bottom plate 203, 2 inside with the wall 201 reaches the basement membrane 202 divide into first district 204, second district 205 and third district 206, the bottom plate 203 is located the tip of pipe shaft 2, cut off 201 with be between the basement membrane 202 the second district 205, the basement membrane 202 with be between the bottom plate 203 the third district 206, cut off 201 place circular profile's radius, the radius of basement membrane 202 all with 2's inside radius equals, be equipped with test paper strip storehouse 207 on the pipe shaft 2, the third district 206 with communicate through preformed hole 210 between the test paper strip storehouse 207, preformed hole 210 department is equipped with paraffin shutoff 212, the first area 204 and the second area 205 are provided with preserving fluid, the third area 206 is provided with detecting fluid, the test strip bin 207 is provided with a test strip 208, and the base comprises: the pipe body 2, the propelling body 4 is close to the pipe body 2 one side is equipped with the point pricker 401, locking ring 5 is established the outside of propelling body 4, the external diameter of locking ring 5 is greater than the internal diameter of pipe body 2.
In this embodiment, the storage solution, the detection solution and the test strip 208 are loaded into the sampling amplification tube, sealed and shipped. The locking ring 5 is used for fixing and temporarily locking the propelling body 4, the paraffin plugging 212 has a good sealing effect, paraffin does not react with common chemical reagents, and the influence on a detection result after melting can be ignored. Before use, the sampling amplification tube is vertically placed; when in use, the upper cover 1 is unscrewed, a sampling cotton swab can be inserted into the preservation solution in the tube body 2 to be fully contacted, elution and sample retention are completed, the upper cover 1 is screwed, the preservation solution is shaken uniformly, the sampling amplification tube is transversely arranged, the gap side of the partition 201 is arranged upwards, the liquid level in the second area 205 is kept level with the height of the partition 201 through the interception function of the partition 201, the volume of the liquid in the second area 205 is ensured to be fixed, the locking ring 5 is torn off during detection, the sharp pricker 401 is screwed downwards to push the propelling body 4, the sharp pricker 401 pierces a sharp pricker hole 211 on the bottom membrane 202, the propelling body 4 is withdrawn in the opposite direction, the sampling amplification tube is transversely arranged in the oven, the liquid in the second area 205 flows into the third area 206 along the sharp pricker hole 211 to be mixed with the detection solution, and amplification reaction occurs at 60 ℃, after the reaction is completed, the reaction is heated to 80 ℃, the sealing paraffin at the position of the preformed hole 210 is melted, the preformed hole 210 is opened, the reaction solution in the third area flows into the test strip bin 207 and meets the test strip 208, and the test strip 208 changes. Generally, the visual judgment can be performed according to the color change, the test strip bin 207 is made of transparent materials, the test strip 208 is provided with a control C line and a detection T line, if the control C line and the detection T line are colored, the detection result is positive, and if only the control C line is colored, the detection result is negative.
The partition 201 is a part of a circle, a dam is formed inside the tube body 2 to perform a blocking function, when the sampling amplification tube is placed transversely, a gap side of the partition 201 is upward, a certain volume of liquid storage space is formed between the partition 201 and the bottom film 202, the liquid storage space exists in the second region 205, and the sampling amplification tube does not flow to the first region 204 when placed transversely. The advancing stroke of the pusher 4 is preferably controlled so as not to touch the partition 201 by piercing the bottom film 202. The preservation solution is used for eluting and preserving the collected sample, the detection solution is used for reacting with the specific virus to achieve the detection purpose, and the components of the preservation solution and the detection solution are not the core scheme of the application.
Example 2: in addition to the structure of embodiment 1, the bottom plate 203 is provided with a guide hole 209 for the spike cone 401 to pass through, the guide hole 209 is close to the inner wall of the tube body 2, and the projection of the guide hole 209 parallel to the axis of the tube body 2 falls on the partition 201. The guide hole 209 is provided with a sealing structure, so that the detection liquid cannot be leaked out. The arrangement of the guide hole 209 limits the pushing position of the prick cone 401, and the device has a guiding function, and during operation, the prick cone 401 only needs to be aligned to the guide hole 209 to push the pushing body 4, so that the error operation that the prick penetrates the upper part of the bottom film 202 and the detection cannot be performed is avoided, and the accuracy of the detection operation is improved.
Example 3: on the basis of the structure of embodiment 1, the base further includes: and one end of the connecting piece 3 is connected with the pipe body 2, one end of the connecting piece is provided with the propelling body 4, and the outer diameter of the propelling body 4 is larger than the inner diameter of the pipe body 2 and smaller than the inner diameter of the connecting piece 3. The length design of the connecting piece 3 needs to meet the requirement that the sharp pricker 401 pierces the bottom film 202 and does not touch the propelling stroke of the partition 201, the connecting piece 3 is arranged to facilitate the control of the stroke of the propelling body 4, and the operation standardization is improved.
Further, the locking ring 5 is rigidly connected with a convex locking nose 501 at the ring opening. When the detection is convenient, the locking ring 5 is convenient to tear off, and the restraint of the propelling body 4 is removed.
Further, a support seat 213 is disposed on the tube body 2, and the support seat 213 is located on the same side as the test strip bin 207. The support seats 213 are provided to facilitate the horizontal arrangement of the sample amplification tubes, and one or more support seats 213 may be provided.
The utility model discloses a virus nucleic acid integration detection device integrates sampling, amplification and detection, can realize the on-the-spot quick check, has easy and simple to handle, sensitivity is high, the short advantage of check-out time.
The foregoing is only a preferred embodiment of the present invention and is not intended to limit the present invention in any way, and those skilled in the art may make modifications and changes to the equivalent embodiments using the teachings of the present invention. However, any simple modification, equivalent change and modification made to the above embodiments according to the technical substance of the present invention still belong to the protection scope of the technical solution of the present invention.
Claims (5)
1. An integrated test paper strip-shaped sampling amplification tube comprises: upper cover (1), pipe shaft (2) and base, pipe shaft (2) one end is installed on the base, the other end with upper cover (1) threaded connection, its characterized in that, pipe shaft (2) is inside to be close to base department is equipped with semicircular wall (201), circular basement membrane (202) and circular bottom plate (203), pipe shaft (2) inside with wall (201) and basement membrane (202) divide into first district (204), second district (205) and third district (206), bottom plate (203) are located the tip of pipe shaft (2), wall (201) with be between basement membrane (202) second district (205), basement membrane (202) with be between bottom plate (203) third district (206), the radius of wall (201) place circular profile, the radius of basement membrane (202) all with the inside radius of pipe shaft (2) equals, be equipped with test paper strip storehouse (207) on pipe shaft (2), third district (206) with through preformed hole (210) intercommunication between test paper strip storehouse (207), preformed hole (210) department is equipped with paraffin shutoff (212), first district (204) with there is preservative fluid in second district (205), there is detection liquid in third district (206), test paper strip storehouse (207) embeds there is test paper strip (208), the base includes: the pipe body (2) one side is equipped with sharp awl (401), establish locking circle (5) the outside of propelling body (4), the external diameter of locking circle (5) is greater than the internal diameter of pipe body (2).
2. The integrated test strip-type sampling amplification tube according to claim 1, wherein the bottom plate (203) is provided with a guide hole (209) for the puncture cone (401) to pass through, the guide hole (209) is close to the inner wall of the tube body (2), and a projection of the guide hole (209) parallel to the axis of the tube body (2) falls on the partition (201).
3. The integrated test strip-type sampling amplification tube of claim 1, wherein the base further comprises: the connecting piece (3), connecting piece (3) one end with pipe shaft (2) are connected, and the one end is being installed impel body (4), the external diameter of impel body (4) is greater than the internal diameter of pipe shaft (2), is less than the internal diameter of connecting piece (3).
4. The integrated test strip-type sampling amplification tube according to claim 1, wherein the locking ring (5) is rigidly connected to a protruding locking nose (501) at the opening of the ring.
5. The integrated test strip-type sampling and amplification tube of claim 1, wherein the tube body (2) is provided with a support seat (213), and the support seat (213) is located on the same side of the test strip chamber (207).
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
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CN202121323590.3U CN215162693U (en) | 2021-06-15 | 2021-06-15 | Integrated test paper strip type sampling amplification tube |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
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CN202121323590.3U CN215162693U (en) | 2021-06-15 | 2021-06-15 | Integrated test paper strip type sampling amplification tube |
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CN215162693U true CN215162693U (en) | 2021-12-14 |
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CN202121323590.3U Active CN215162693U (en) | 2021-06-15 | 2021-06-15 | Integrated test paper strip type sampling amplification tube |
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2021
- 2021-06-15 CN CN202121323590.3U patent/CN215162693U/en active Active
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