CN113649098A - Microfluidic detection assembly, sample mixing device and application thereof - Google Patents
Microfluidic detection assembly, sample mixing device and application thereof Download PDFInfo
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- CN113649098A CN113649098A CN202111206908.4A CN202111206908A CN113649098A CN 113649098 A CN113649098 A CN 113649098A CN 202111206908 A CN202111206908 A CN 202111206908A CN 113649098 A CN113649098 A CN 113649098A
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- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
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Abstract
The invention provides a microfluidic detection assembly, which comprises a detection card and a sample mixing device capable of being plugged with the detection card, wherein the sample mixing device comprises: a body; at least one mixing pool arranged at intervals in the body and used for mixing at least two liquid samples, wherein the inlet of the mixing pool is positioned at the first side of the body; the first end of the flow channel is respectively communicated with the mixing pool through a capillary pipeline or a through hole; when the detection card is plugged on the mixing device, the flow channels are respectively communicated with the reaction cavities in the detection card, and when the assembly is subjected to the action of centrifugal force, the mixed liquid in the mixing pool enters the corresponding reaction cavities through the capillary pipelines or the through holes and the flow channels. The micro-fluidic detection assembly can uniformly mix the liquid sample under the external force matching, the accuracy of a test result is improved, and meanwhile, the sample mixing device in the assembly is suitable for simultaneously adding samples to a plurality of reaction cavities, so that the operation is simple and the cross infection is avoided.
Description
Technical Field
The invention relates to the field of medical detection, in particular to a microfluidic detection assembly, a sample mixing device and application thereof.
Background
In some antibody or antigen detection tests (e.g., anti-human globulin test, irregular antibody screening test, cross-matching test), two or more liquid samples are mixed, and the mixed solution obtained by mixing is reacted with a reagent to observe the result.
In the prior art, two or more liquid samples to be tested are usually directly added to a test card, and the reaction result is observed. For example, in the current direct anti-human globulin test, plasma and cells are respectively added into a reaction chamber, so that the phenomenon of uneven mixing of the plasma and the cells easily exists, and the reaction between a liquid sample and a reagent is further influenced, so that the accuracy of the test result is influenced.
On the other hand, in general, a plurality of samples (same or different) are detected simultaneously, if liquid samples are mixed separately for each sample, and then the mixed solution is added into the detection card, the liquid samples are mixed for many times, the detection card needs to be added with samples for many times, the operation is frequent, and the workload of operators is increased; moreover, when an operator is wrong in the operation process (for example, the mixed liquid to be detected is splashed onto the detection card or the liquid to be detected is splashed into other reaction cavities), the detection card or the mixed liquid added into the detection card is often polluted, the detection card needs to be replaced again and samples need to be prepared, on one hand, the waste of materials is caused, and on the other hand, the detection efficiency is reduced.
Therefore, a testing device capable of uniformly mixing a liquid sample (sample to be tested) and facilitating sample application is needed.
Disclosure of Invention
The invention aims to provide a microfluidic detection assembly and a sample mixing device, which partially solve or alleviate the defects in the prior art, realize the mixing of a liquid sample to be detected and the sample adding of a detection card, avoid cross contamination, simplify the operation process and facilitate the automation of sample adding and detection.
In order to solve the technical problems, the invention specifically adopts the following technical scheme: a microfluidic detection assembly comprising: the device comprises a detection card and a sample mixing device which can be plugged with the detection card;
wherein the sample mixing device comprises:
a body;
at least one mixing cell spaced within the body for mixing at least two liquid samples, the mixing cell having an inlet at a first side of the body;
the first end of the at least one circulation channel is respectively communicated with the corresponding mixing pool through a micro channel;
when the detection card is plugged on the sample mixing device, at least one flow channel is respectively communicated with the corresponding reaction cavity in the detection card, and when the assembly is under the action of centrifugal force, the mixed liquid in the mixing pool flows into the flow channel through the micro channel and enters the reaction cavity through the flow channel.
In some embodiments of the invention, the microchannel is a through-hole or a capillary channel.
In some embodiments of the invention, the second end of the flow-through channel extends outside the body and is provided with a pointed tip for piercing a sealing membrane on the reaction chamber;
when the test card is inserted into the sample mixing device, the pointed tip pierces the sealing membrane such that at least one of the mixing wells communicates with the corresponding reaction chamber.
In some embodiments of the present invention, the mixing tank comprises a first mixing zone and a second mixing zone in communication, wherein the width of the second mixing zone gradually decreases in a direction from the first end of the second mixing zone to the second end of the second mixing zone.
In some embodiments of the invention, the inlet edge of the mixing cell is provided with a barrier structure for preventing splashing of the liquid sample.
In some embodiments of the present invention, the body is sheet-shaped or plate-shaped, and the at least one mixing tank is disposed at intervals in a width direction of the body inside the body.
In some embodiments of the present invention, a diversion trench for diversion is disposed in the second mixing region, and the diversion trench is communicated with the micro channel.
In some embodiments of the invention, the sample mixing device further comprises: the two clamping openings are formed by side walls extending from two sides of the body and are used for being inserted with the detection card; when the detection card is pushed into the clamping opening, the flow channel is communicated with the reaction cavity.
In some embodiments of the present invention, a limit protrusion is disposed on the side wall, a limit slot capable of matching with the limit protrusion is disposed on the detection card, and when the limit slot matches with the limit protrusion, the detection card is clamped with the clamping opening.
In some embodiments of the present invention, a limit slot is disposed on the side wall, a limit protrusion capable of matching with the limit slot is disposed on the detection card, and when the limit slot matches with the limit protrusion, the detection card is clamped with the clamping opening.
In some embodiments of the present invention, a first clamping position and a second clamping position are disposed on a side wall of the clamping opening at intervals along an extending direction of the side wall;
the detection card is movably arranged at the second clamping position relative to the clamping opening;
when the detection card moves from the second clamping position to the first clamping position under the action of external acting force, the circulation channel is communicated with the reaction cavity in the detection card.
In some embodiments of the invention, one or both sides of the detection card are provided with a second clamping position and a first clamping position at intervals along a direction gradually far away from the mixing pool;
the detection card is movably arranged at the second clamping position relative to the clamping opening;
when under the action of external acting force, the detection card is moved from the second clamping position to the first clamping position, and the circulation channel is communicated with the reaction cavity in the detection card.
In some embodiments of the present invention, one or both sides of the detection card are respectively provided with a limit protrusion, and accordingly, the card opening includes: the guide rail is used for providing a moving path for the detection card, and a first limiting clamping groove and a second limiting clamping groove which can be matched with the limiting clamping protrusions are respectively arranged at the first clamping position corresponding to the clamping opening and the second clamping position corresponding to the clamping opening on the guide rail; when the limiting clamping protrusion is matched with the second limiting clamping groove, the detection card and the clamping opening are clamped at the second clamping position of the clamping opening; when under the effect of external acting force, make spacing protruding breaking away from during the spacing draw-in groove of second, the detection card can be followed the guide rail is to being close to the circulation passageway direction removes, and works as spacing protruding removal of card extremely first spacing draw-in groove, and with when first spacing draw-in groove matches, the detection card with joint opening joint in the joint open-ended first joint position.
In some embodiments of the present invention, one or both sides of the detection card are respectively provided with a limit protrusion, and accordingly, the card opening includes: the guide rail is used for providing a moving path for the detection card, and first limiting clamping grooves which can be matched with the limiting clamping protrusions are respectively arranged at first clamping positions corresponding to the clamping openings on the guide rail; the tail end of the limiting clamping protrusion is fixedly connected with the second clamping position, corresponding to the clamping opening, on the guide rail, and a breaking line which is easy to break is arranged at the joint of the limiting clamping protrusion and the second clamping position of the clamping opening; when under the effect of external acting force, make spacing calorie protruding with during the guide rail disconnection, the detection card can be followed the guide rail is to being close to the circulation passageway direction removes, and works as spacing calorie of protruding removal extremely first spacing draw-in groove department, and with when first spacing draw-in groove matches, the detection card with joint opening joint in the joint open-ended first joint position.
In some embodiments of the present invention, a first limiting slot and a second limiting slot are respectively disposed at one side or both sides of the detection card corresponding to the first clamping position of the detection card and the second clamping position of the detection card; accordingly, the clamping opening comprises: the sliding guide rail is used for providing a sliding path for the detection card, and a limiting card protrusion which can be matched with the first limiting card slot and the second limiting card slot is arranged on the sliding guide rail;
when the limiting clamping protrusion is matched with the second limiting clamping groove, the detection card and the clamping opening are clamped at a second clamping position of the detection card;
when under the effect of external acting force, make spacing calorie of protruding breaking away from during the spacing draw-in groove of second, the detection card can be followed the guide rail is to being close to the circulation passageway direction removes, and works as first spacing draw-in groove removes to spacing calorie of protruding position, and with when spacing calorie of protruding phase match, the detection card with joint opening joint in the first joint position of detection card.
In some embodiments of the invention, the snap opening comprises: the guide rail is used for providing a sliding path for the detection card, and a limiting card protrusion is arranged on the guide rail; correspondingly, a first limiting clamping groove which can be matched with the limiting clamping protrusion is arranged at one side or two sides of the detection card corresponding to the first clamping position of the detection card; the tail end of the limiting clamp protrusion is fixedly connected to the second clamping position on the detection card, and a breaking line which is easy to break is arranged at the connection position of the limiting clamp protrusion and the second clamping position of the detection card;
when the limiting clamp protrusion is disconnected from the detection clamp under the action of an external acting force, the detection clamp can move towards the direction close to the flow channel along the guide rail; and work as first spacing draw-in groove removes extremely spacing protruding position department of card, and with when spacing protruding phase-match of card, the detection card with joint opening joint in the first joint position of detection card.
In a second aspect of the present invention, there is also provided a sample mixing apparatus comprising:
a body;
at least one mixing cell spaced within the body for mixing at least two liquid samples, the mixing cell having an inlet at a first side of the body;
the first end of the at least one circulation channel is respectively communicated with the corresponding mixing pool through a micro channel.
In some embodiments of the invention, the microchannel is a through-hole or a capillary channel.
In some embodiments of the invention, the second end of the flow channel extends outside the body and is provided with a pointed tip for puncturing.
In some embodiments of the present invention, the body is sheet-shaped or plate-shaped, and the at least one mixing tank is disposed at intervals in a width direction of the body inside the body.
In some embodiments of the invention, the sample mixing device further comprises: the clamping opening is formed by side walls extending from two sides of the body and is used for being inserted with the detection card;
when the detection card is pushed into the clamping opening, the at least one circulation channel is respectively communicated with the corresponding reaction cavity on the detection card.
In some embodiments of the present invention, a limit card protrusion capable of matching with the limit card slot on the detection card is arranged on the side wall; or
The side wall is provided with a limit clamping groove which can be matched with the limit clamping protrusion on the detection card.
In a third aspect of the present invention, there is provided a use of the microfluidic detection assembly or the sample mixing device in antibody detection or antigen detection.
Compared with the prior art, the sample mixing device provided by the invention can fully mix (or fully react or combine) the liquid samples under the coordination of external force, so that the phenomenon that the liquid samples are not fully mixed and then react with the reagents is influenced is avoided, and the accuracy of a test result is improved.
Furthermore, the invention also provides a microfluidic detection assembly comprising the detection card and the sample mixing device, the assembly realizes multi-channel sample adding on the detection card by utilizing capillary action and centrifugal force, the operation is simple, and on one hand, cross contamination or detection card contamination caused by misoperation is avoided; on the other hand, the sample adding efficiency is improved, and the automation of sample adding and detection processes is facilitated.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below. Throughout the drawings, like elements or portions are generally identified by like reference numerals. In the drawings, elements or portions are not necessarily drawn to scale. It is obvious that the drawings in the following description are some embodiments of the invention, and that for a person skilled in the art, other drawings can be derived from them without inventive exercise.
FIG. 1 is a schematic view of the upper surface of a sample mixing device according to an exemplary embodiment of the present invention;
FIG. 2 is a perspective view of a microfluidic detection assembly according to an exemplary embodiment of the present invention;
FIG. 3a is a first longitudinal cross-sectional view of a microfluidic detection assembly according to an exemplary embodiment of the present invention;
FIG. 3b is a second longitudinal cross-sectional view of a microfluidic detection assembly according to an exemplary embodiment of the present invention;
FIG. 4 is a schematic diagram of a first perspective structure of a microfluidic detection assembly according to an exemplary embodiment of the present invention;
FIG. 5 is a schematic view of a first internal structure of a sample mixing device according to an exemplary embodiment of the present invention;
FIG. 6 is a schematic diagram of a second internal structure of a sample mixing device according to an exemplary embodiment of the present invention;
FIG. 7 is an enlarged view of area a of FIG. 6;
FIG. 8 is a schematic view of a third internal structure of a sample mixing device according to an exemplary embodiment of the present invention;
FIG. 9 is an enlarged partial schematic view of the structure shown in FIG. 8;
fig. 10 is a schematic diagram of a second perspective structure of a sample mixing device according to an exemplary embodiment of the present invention.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention. It is to be understood that the embodiments described are only a few embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Herein, suffixes such as "module", "part", or "unit" used to denote elements are used only for facilitating the description of the present invention, and have no specific meaning in itself. Thus, "module", "component" or "unit" may be used mixedly.
Herein, the terms "upper", "lower", "inner", "outer", "front", "rear", "one end", "the other end", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience in describing and simplifying the description, but do not indicate or imply that the referred device or element must have a specific orientation, be constructed in a specific orientation, and be operated, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first" and "second" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.
As used herein, unless otherwise expressly specified or limited, the terms "mounted," "disposed," "connected," and the like are to be construed broadly, such that the terms "connected," or "connected," as used herein, may be fixedly connected, detachably connected, or integrally connected; they may be mechanically coupled, directly coupled, indirectly coupled through intervening media, or may be interconnected between two elements. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art.
As used herein, the term "microchannel" refers to a channel that allows the fluid in the mixing chamber to flow into the flow channel only under the action of centrifugal force. Specifically, the microchannel may be a through-hole provided at the bottom of the mixing cell, or a capillary channel connecting the mixing cell and the flow channel. Of course, the through-going hole can also be realized by using a capillary channel.
Example one
Referring to fig. 2, 3b and 4, a microfluidic detection assembly according to an exemplary embodiment of the present invention comprises: a detection card 6 and a sample mixing device which can be inserted with the detection card 6; wherein, sample mixing arrangement includes: a body 1; at least one mixing pool 2 which is arranged in the body 1 at intervals and used for mixing at least two liquid samples, wherein the inlet of the mixing pool 2 is positioned at the first side of the body 1; at least one flow channel 4 arranged in the body 1 at intervals, wherein the first end of the at least one flow channel 4 is respectively communicated with the corresponding mixing pool 2 through a micro channel 3; when the detection card 6 is inserted into the sample mixing device, at least one flow channel 4 is respectively communicated with the corresponding reaction cavity 63 in the detection card 6, and when the assembly is subjected to centrifugal force, the mixed liquid in at least one mixing pool 2 enters the corresponding reaction cavity 63 through the micro channel 3 and the flow channel 4.
In some embodiments, the microchannels 3 are through-going holes, such that the mixed liquid in the mixing chamber flows into the flow-through channel through the through-going holes only under the action of centrifugal force, thereby ensuring that the liquid to be mixed in the mixing chamber is sufficiently mixed (e.g., under the action of a vibrator or the like).
Further, in some embodiments, the micro-channel or the through hole is a capillary channel, so that the sample mixing device is not affected by an external force, and the mixed liquid in the mixing pool of the device does not flow out of the micro-channel 3 or the through hole, thereby ensuring that the liquid to be mixed in the mixing pool can be sufficiently/uniformly mixed (for example, under the action of an oscillator and the like).
Specifically, in some embodiments of the present invention, referring to fig. 1-10, the sample mixing device includes at least one mixing cell 2 spaced apart from and disposed in a body 1 for mixing a liquid sample, an inlet of the mixing cell 2 (i.e., a sample loading port of the mixing cell) is located on a first side (e.g., an upper surface) of the body 1, and a corresponding number of flow channels 4 spaced apart from and disposed in the body 1, and the flow channels 4 respectively correspond to and communicate with the mixing cells 2.
In some embodiments, the second end of the mixing well 2 (i.e. the end of the mixing well 2 close to the flow channel 4) is provided with a microchannel 3, the second end of the mixing well 2 and the flow channel 4 are communicated through the microchannel 3, and when the detection card 6 is inserted into the sample mixing device, at least one flow channel 4 is communicated with the corresponding reaction chamber 63 of the detection card 6, i.e. the mixing wells 2 of the sample mixing device are respectively communicated with the corresponding reaction chambers 63.
It will be appreciated that in some embodiments, the test card and the sample mixing device in the assembly are independent of each other before use, and in use, the liquid sample to be tested is first introduced into the mixing well 2 through the inlet, and then the sample mixing device is placed in an oscillator (e.g., an ultrasonic oscillator) by external force, for example, so that the liquid sample in the mixing well 2 is uniformly mixed by oscillation to obtain a mixed solution. Because the micro channel connected with the mixing pool adopts the through hole (namely the capillary through hole) or the capillary pipeline, when the sample mixing device oscillates under the action of external force, the mixed liquid in the mixing pool can not flow out of the micro channel.
Further, the detection card 6 is inserted into the sample mixing device, so that the mixing chambers 2 of the sample mixing device are respectively communicated with the corresponding reaction chambers 63, then the microfluidic detection assembly formed by the detection card 6 and the sample mixing device is placed into a centrifugal clamping groove of a centrifuge for centrifugation, under the action of centrifugal force, the mixed liquid in the mixing chambers 2 rapidly flows through the flow channel 4 through the micro channel (capillary tube) 3 and enters the corresponding reaction chambers 63, the reaction chambers 63 are connected with the reagent storage region 64 (in which reagents are stored in advance), and the mixed liquid can react with the reagents in the reaction chambers.
Preferably, in some embodiments of the present invention, 6 mixing wells are provided in the sample mixing device, which is intended to be used as 2-person sets for performing various experimental operations, for example, in antibody screening tests, wherein three mixing wells are 1-person set, and the other three mixing wells are 2-person set, and the cells added in each 1-person set are anti-sieve cells 1, anti-sieve cells 2, and anti-sieve cells 3; when the device is applied to a cross matching test, the device can be used as 3 persons, and 1 person is used for each two mixing tanks; the direct anti-human globulin test was performed on 6 aliquots, i.e., 1 aliquot of cells was added to each mixing well.
Of course, considering that in some cases (e.g., antibody screening test or cross-matching test), when only 1-person sample is to be tested, 6 mixing wells are not needed, and to avoid waste, only 3 mixing wells (preferably, applied to 1-person antibody screening test) or 2 mixing wells (preferably, applied to 1-person cross-matching test) or 1 mixing well (preferably, applied to 1-person direct anti-proteo-globulin test) may be provided on the sample mixing device; of course, the mixing pools with corresponding numbers can be set according to different application scenarios.
In some embodiments of the present invention, referring to fig. 4 and 5, the body 1 of the sample mixing device is generally plate-like or block-like, i.e., its dimensions in the direction of length L and width W are much larger than the dimensions in the direction of thickness (or height) H.
Preferably, in some embodiments of the present invention, referring to fig. 1, a plurality of mixing wells 2 and, correspondingly, a plurality of flow channels 4 are provided in the sample mixing apparatus, wherein the second ends of the plurality of flow channels 4 extend outside the body 1 and are provided with pointed tips 41 for piercing a sealing membrane (e.g., a thin film material) on the reaction chamber 63; when the detection card 6 is inserted into the sample mixing device, the pointed tip 41 pierces the sealing film, so that the mixing well 2 communicates with the reaction chamber 63.
Preferably, in some embodiments of the present invention, the mixing tank 2 comprises a first mixing zone 21 and a second mixing zone 22 which are in communication, wherein the width of the second mixing zone 22 gradually decreases in a direction from the first end of the second mixing zone 22 to the second end of the second mixing zone 22.
Referring to fig. 6-9, in some embodiments of the present invention, the mixing tank 2 comprises a first mixing zone 21 and a second mixing zone 22, the first mixing zone 21 is in communication with the second mixing zone 22, the width (i.e. the length in the W direction) of the second mixing zone 22 gradually decreases from the first end (i.e. the end connected with the first mixing zone 21) thereof to the second end (i.e. the end near the flow-through channel 4), and the height (or depth, i.e. the length in the H direction) of the second mixing zone 22 also gradually decreases from the first end to the second end thereof, so that the cross-section of the second mixing zone is approximately triangular or approximately triangular and rectangular superimposed (for example, referring to fig. 3b, the depth of the second mixing zone is gradually decreased from the first end to the second end along a dotted line L1), wherein the second mixing zone 22 functions to guide and collect the mixed liquid, by gradually reducing the width and depth of the second mixing zone 22, the mixed liquor will gradually pool to the second end of the second mixing zone 22.
Further, in order to make the mixed liquid in the mixing tank 2 smoothly enter the flow channel 4, in some embodiments of the present invention, a flow guide groove 23 for guiding the mixed liquid is provided in the second mixing zone 22, and the flow guide groove 23 is communicated with the micro channel 3 (e.g., a through hole or a capillary). Specifically, the guiding groove 23 extends from one end communicating with the micro channel to a second end of the second mixing region 22 along the length direction (or L direction of the body) of the second mixing region 22 for a certain length, so that the second mixing region 22 is approximately funnel-shaped.
In some embodiments, the microchannel 3 is a spherical through-hole with a small enough diameter to allow the liquid to pass through only under a certain centrifugal force, and specifically, the spherical through-hole is located between the guide groove 23 and the flow channel 4.
In other embodiments, the microchannel is a capillary channel, and both ends of the capillary channel are respectively communicated with the diversion trench 23 and the flow channel 4.
Further, for convenience of processing, the capillary channel extends for a certain length from one end communicated with the diversion trench along the direction of the body H and then is communicated with the flow channel, namely the capillary channel is perpendicular to the first side surface of the body. Of course, the axial direction of the capillary channel may also have an angle with the H direction (or the first side surface) of the body.
When the microfluidic detection assembly is subjected to centrifugal force, all the mixed liquid in the mixing pool is collected in the second mixing region 22, enters the microchannel 3 from the diversion trench 23 of the second mixing region 22, enters the flow channel 4, and finally enters the corresponding reaction chamber 63.
Preferably, in some embodiments of the present invention, the body is sheet-shaped or plate-shaped, and the plurality of mixing pools 2 are arranged at intervals in the width direction of the body 1 (i.e., the W direction of the body) inside the body 1.
Preferably, to facilitate the use of the test card 6 in conjunction with a sample mixing device, in some embodiments of the present invention, the sample mixing device further comprises: two clamping openings formed by side walls 5 extending from two sides of the body 1 and used for being inserted with a detection card 6; when the detection card 6 is pushed into the card-holding opening, the plurality of flow channels 4 communicate with the corresponding reaction chambers 63, respectively.
Preferably, in order to facilitate the matching of the detection card 6 and the sample mixing device, and to prevent the detection card 6 or the sample mixing device from falling off when a user transfers the assembly, in some embodiments of the present invention, the side wall 5 is provided with a limit card projection 51, and the detection card is provided with a limit card slot capable of matching with the limit card projection; or the side wall 5 is provided with a limit clamping groove, the detection card is provided with a limit clamping protrusion matched with the limit clamping groove, and when the limit clamping groove is matched with the limit clamping protrusion, the detection card is clamped with the clamping opening.
Further, in order to enable the sealing film of the reaction cavity 63 of the detection card 6 to correspond to the (pointed end 41 of the) flow channel 4 of the sample mixing device (i.e. correspond to the direction H of the body), the detection card 6 and the sample mixing device are prevented from being dislocated, so that the mixed liquid is splashed outwards, the test result is further affected, and the experimental environment is polluted, in some embodiments, the positioning guide rail 52 is arranged on at least one side wall 5 of the clamping opening, and the positioning guide groove matched with the positioning guide rail 52 is arranged at the corresponding position of the detection card 6.
Of course, in other embodiments, a positioning guide groove may be provided on at least one side wall 5 of the card-engaging opening, and a positioning guide rail matched with the positioning guide groove may be provided at a corresponding position on the detection card 6.
Further, in some embodiments, one side or both sides of the detection card 6 are provided with a second clamping position and a first clamping position at intervals along a direction gradually away from the mixing pool 2; the detection card 6 is arranged at a second clamping position of the detection card in a mode of moving relative to the clamping opening; when the test card 6 is moved from the second clamping position to the first clamping position by an external force (the moving direction is shown by an arrow in fig. 2), the flow channel 4 is communicated with the reaction chamber 63 in the test card.
Specifically, in some embodiments of the present invention, one or both sides of the detection card 6 are respectively provided with a first limit slot 61 and a second limit slot 62 corresponding to a first clamping position and a second clamping position of the detection card; accordingly, the snap opening comprises: the sliding guide rail is used for providing a sliding path for the detection card 6, and a limiting card protrusion which can be matched with the first limiting card slot and the second limiting card slot is arranged on the sliding guide rail; when the limiting clamping protrusion is matched with a second limiting clamping groove of the detection card, the detection card 6 and the clamping opening are clamped at a second clamping position of the detection card; when under the effect of external acting force for spacing protruding the second spacing draw-in groove that breaks away from the detection card of card, detect card 6 and can follow the guide rail and remove to 4 directions of being close to circulation passageway, and when first spacing draw-in groove removed to spacing protruding position of card to when cooperating with spacing protruding phase of card, detect card 6 and joint opening joint in the first joint position of detecting the card.
In other embodiments, the snap opening comprises: a guide rail (specifically, two side walls 5 extending from the body, or a guide rail is arranged on the two side walls 5 along the length direction thereof) for providing a moving path for the detection card 6, and a limit card protrusion 51 is arranged on the guide rail; correspondingly, one or both sides of the detection card 6 are provided with a first limit clamping groove 61 corresponding to the limit clamping protrusion at a first clamping position of the detection card, and the end of the limit clamping protrusion 51 (i.e. the end away from the guide rail and corresponding to the detection card 6) is fixedly connected to a second clamping position on the detection card 6, and the connection is provided with a breaking line which is easy to break.
In the initial state, the position-limiting protrusion 51 on the clamping opening and the detection card 6 are fixedly connected to the second clamping position of the detection card, and at this time, the pointed end 41 of the flow channel 4 does not pierce the thin film material on the reaction chamber 63 of the detection card 6. In practice, the pointed end 41 is actually spaced from the membrane of the reaction chamber 63 by a distance e, and specifically, the distance is only required to be sufficient that the pointed end 41 cannot pierce the membrane of the open end of the reaction chamber 63.
When the limiting clamp protrusion 51 is disconnected from the guide rail under the action of an external acting force, the detection card 6 can move towards the direction close to the flow channel 4 along the guide rail; and when the first limit slot 61 on the detection card 6 moves to the position of the limit projection 51 on the guide rail and is matched with the limit projection 51 under the action of an external acting force, the detection card 6 is clamped at the first clamping position of the detection card 6 with the clamping opening, and the pointed tail end 41 of the flow channel 4 pierces through the film material on the reaction cavity 63 on the detection card 6.
In other embodiments of the present invention, the sidewall 5 of the clamping opening is provided with a first clamping position and a second clamping position at intervals along the extending direction of the sidewall 5; the detection card 6 is arranged at the second clamping position in a mode of moving relative to the clamping opening; when the detection card 6 is moved from the second clamping position to the first clamping position by an external force, the flow channel 4 of the sample mixing device is in communication with the reaction chamber 63 of the detection card 6.
In some embodiments, one or both sides of the detection card 6 are respectively provided with a limit protrusion, and accordingly, the card opening includes: the guide rail is used for providing a moving path for the detection card 6, and a first limiting clamping groove and a second limiting clamping groove which can be matched with the limiting clamping protrusions are respectively arranged on the guide rail corresponding to a first clamping position and a second clamping position on the clamping opening; when the limiting clamp protrusion on the detection card 6 is matched with the second limiting clamp groove on the clamping position, the detection card 6 and the clamping opening are clamped at the second clamping position on the clamping opening; when the effort effect is down outside for spacing protruding second spacing draw-in groove that breaks away from on the joint opening of card, detect card 6 and can follow the guide rail and remove to being close to 4 directions of circulation passageway, and move extremely when spacing protruding removal of card first spacing draw-in groove to when cooperating with first spacing draw-in groove, detect card 6 with joint opening joint is in joint open-ended first joint position.
In other embodiments, one or both sides of the detection card are respectively provided with a limit card protrusion, and accordingly, the card opening comprises: the guide rail is used for providing a moving path for the detection card, and first limiting clamping grooves which can be matched with the limiting clamping protrusions are respectively arranged on the guide rail corresponding to first clamping positions on the clamping opening; the tail end of the limiting clamping protrusion is fixedly connected with a second clamping position on the guide rail, which corresponds to the clamping opening, and a breaking line which is easy to break is arranged at the joint of the limiting clamping protrusion and the second clamping position of the clamping opening; when under the effect of external acting force for spacing protruding and the guide rail disconnection, the detection card can be followed the guide rail and removed to being close to 4 directions of circulation passageway, and when spacing protruding removal to the first spacing draw-in groove departments of joint open-ended to when matching with this first spacing draw-in groove, detection card and joint opening joint in first joint position (when initial condition promptly, spacing protruding fixed connection of card is in second joint position department on the guide rail).
Preferably, in some embodiments of the present invention, the snap opening comprises: a guide rail for providing a sliding path for the detection card 6, and a limit card projection 51 is arranged on the guide rail; correspondingly, one side or two sides of the detection card 6 corresponding to the first clamping position are provided with first limiting clamping grooves which can be matched with the limiting clamping protrusions; the tail end of the limiting clamp protrusion is fixedly connected to a second clamping position on the detection card 6, and a breaking line which is easy to break is arranged at the connection position of the limiting clamp protrusion and the second clamping position; when the limiting clamp is disconnected from the detection clamp under the action of an external acting force, the detection clamp can move towards the direction close to the flow channel along the guide rail; and when the first limiting clamping groove moves to the position of the limiting clamping protrusion and is matched with the limiting clamping protrusion, the detection card is clamped with the clamping opening at the first clamping position.
Specifically, in some embodiments of the present invention, the test card 6 is installed in the sample mixing apparatus at the second clamping position of the clamping opening when the assembly is shipped (or in the initial state). For example, the detection card 6 is fixedly connected with the second clamping position through a limiting clamping protrusion 51 arranged on the detection card, and a breaking line which is easy to break is arranged at the connection position. In this state, the pointed end 41 of the flow channel 4 has a distance e from the sealing membrane in the reaction chamber 63, which is slightly smaller than or equal to the spacing between the first latching position and the second latching position, i.e. the pointed end 41 has not pierced the sealing membrane in the reaction chamber 63. And when the connection is broken along the breaking line, and when the detection card 6 moves from the second clamping position to the first clamping position (for example, the limit card projection is in snap fit with the limit card slot), the pointed end 41 of the flow channel 4 can pierce the sealing film of the reaction chamber 63.
In other embodiments, when the module is shipped, the detection card and the sample mixing device are independent from each other, but after the first assembly, the detection card 6 is clamped on the sample mixing device (specifically, clamped at the second clamping position of the clamping opening by the snap fit between the limiting card protrusion 51 and the limiting card slot), and similarly, a certain distance e is provided between the pointed end 41 of the flow channel 4 and the film on the reaction chamber 63, and the distance is slightly smaller than or equal to the distance between the first clamping position and the second clamping position; and when the detection card 6 is moved from the second clamping position to the first clamping position (for example, the limiting clamping protrusion is in snap fit with the limiting clamping groove) under the action of an external force, the pointed end 41 of the flow channel 4 can pierce the membrane on the microcolumn.
Specifically, in some embodiments of the present invention, the detection card 6 has a plurality of reaction chambers, and a reagent storage region (in which a reagent for reaction is stored in advance) communicating with the reaction chambers, and referring to fig. 2 and 4, the open ends of these reaction chambers 63 correspond to the positions of the pointed distal ends 41 of the flow-through channels 4. When the test card 6 is pushed into the card opening, the pointed tip 41 of each flow channel 4 can pierce the film material covering the open end of each reaction chamber 63. The detection card 6 is corresponding to the limit card projection 51, and at least one group of limit card slots are arranged on two sides of the detection card. For example, when two sets of limiting slots are provided on the detection card 6, one of the limiting slots (i.e. the first limiting slot 61 at the first clamping position on the detection card) cooperates with the limiting projection 51 to make the pointed end 41 of the flow channel 4 in a state of piercing through the film material, and the other limiting slot (i.e. the second limiting slot at the second clamping position on the detection card) may be slightly higher, so that when they cooperate with the limiting projection 51, they only play a role of combining the detection card 6 with the sample mixing device of the present invention, and at this time, the pointed end 41 of the flow channel 4 does not pierce through the film material on the detection card 6.
In use, the test card 6 is inserted into the card-receiving opening and pushed upward with force so that the pointed ends 41 of the flow channels 4 just pierce the cover film material in the reaction chambers 63. The assembly is then placed in a centrifuge and the mixed liquor in the mixing well 2 is centrifuged through the microchannels (e.g., through-holes or capillary channels) 3 and the flow-through channels 4 into the corresponding reaction chambers 63 in the test card 6.
Preferably, to avoid splashing of the liquid sample during shaking, in some embodiments of the present invention, the sample mixing device further comprises: the inlet edge of the mixing basin 2, i.e. where the mixing basin 2 is connected to the first side of the body 1, is provided with a barrier structure (not shown in the figures) for preventing splashing of the liquid sample.
Preferably, in some embodiments of the invention, the blocking means are formed by a set of projections extending from the inlet edge towards the outside of the body 1.
Example two
In a second aspect of the present invention, there is also provided a sample mixing device, see fig. 5-10, comprising: a body 1;
at least one mixing pool 2 which is arranged in the body 1 at intervals and used for mixing at least two liquid samples, wherein the inlet of the mixing pool 2 is positioned at the first side of the body 1; at least one flow channel 4 is arranged in the body 1 at intervals, and the first end of the at least one flow channel 4 is respectively communicated with the corresponding mixing pool 2 through the micro-channel 3.
When the test card 6 is attached to the sample mixing device, at least one flow channel 4 is in communication with a corresponding reaction chamber 63 in the test card 6, and when the assembly is subjected to centrifugal force, the mixed liquid in at least one mixing well 2 enters the corresponding reaction chamber 63 through a microchannel (e.g., capillary channel or through-hole) 3 and the flow channel 4.
In use, a liquid sample to be measured is added into the mixing tank 2 through the inlet, and then the sample mixing device is placed into an oscillator (e.g., an ultrasonic oscillator) by means of external force, so that the liquid sample in the mixing tank 2 is oscillated and mixed uniformly to obtain a mixed solution.
Furthermore, the detection card 6 matched with the device is inserted into the sample mixing device, so that at least one mixing pool 2 of the sample mixing device is respectively communicated with the corresponding reaction cavity 63, then a microfluidic detection assembly consisting of the detection card 6 and the sample mixing device is placed into a centrifugal clamping groove of a centrifugal machine for centrifugation, and under the action of centrifugal force, the mixed liquid in the mixing pool 2 rapidly flows through the flow channel 4 through a capillary pipeline or a through hole and enters the corresponding reaction cavity 63.
Preferably, in some embodiments of the present invention, the body 1 is sheet-shaped or plate-shaped, and at least one mixing tank 2 is provided at intervals in the width direction of the body 1 inside the body 1.
In some embodiments of the invention, the second end of the flow-through channel 4 extends outside the body 1 and is provided with a pointed tip 41 for puncturing.
Specifically, when the detection card 6 is inserted into the sample mixing device, the pointed tip 41 pierces the sealing film, so that the mixing well 2 communicates with the reaction chamber 63.
Preferably, in some embodiments of the present invention, the mixing tank 2 comprises a first mixing zone 21 and a second mixing zone 22 which are in communication, wherein the width of the second mixing zone 22 gradually decreases along the first end of the second mixing zone 22 towards the second end of the second mixing zone 22.
Referring to fig. 6-9, in some embodiments of the present invention, the mixing tank 2 includes a first mixing zone 21 and a second mixing zone 22, specifically, in some embodiments, the first mixing zone 21 is in communication with the second mixing zone 22, the width (i.e., the length in the W direction) of the second mixing zone 22 gradually decreases from the first end (i.e., the end connected to the first mixing zone 21) thereof to the second end (i.e., the end near the flow channel 4) thereof, and the height (or depth, i.e., the length in the H direction) of the second mixing zone 22 also gradually decreases from the first end thereof to the second end thereof, so that the cross-sectional area of the second mixing zone is approximately triangular, or approximately triangular and rectangular are superimposed. The second mixing area 22 functions to guide and collect the mixed liquid, and the width and depth of the second mixing area 22 are gradually reduced, so that the mixed liquid is gradually collected to the second end of the second mixing area 22.
Further, in order to make the mixed liquid in the mixing tank 2 smoothly enter the flow channel 4, in some embodiments of the present invention, a flow guide groove 23 for guiding the mixed liquid is provided in the second mixing tank 2, wherein the flow guide groove 23 is communicated with the micro channel 3 (e.g., a through hole or a capillary). Specifically, the guiding groove 23 extends from one end communicating with the micro channel to a second end of the second mixing region 22 along the length direction (or L direction of the body) of the second mixing region 22 for a certain length, so that the second mixing region 22 is approximately funnel-shaped.
Preferably, in some embodiments of the present invention, the method further comprises: two side walls 5 extending from two sides of the body form a clamping opening for being inserted with a detection card 6;
when the test card 6 is pushed into the card-engaging opening, the at least one flow channel 4 communicates with the corresponding reaction chamber 63 of the test card 6.
Preferably, in order to facilitate the matching of the detection card 6 and the sample mixing device, and to prevent the detection card 6 or the sample mixing device from falling off when a user transfers the assembly, in some embodiments of the present invention, the side wall 5 is provided with a limit card projection 51, and the detection card is provided with a limit card slot capable of matching with the limit card projection; or the side wall 5 is provided with a limit clamping groove, and the detection card is provided with a limit clamping protrusion matched with the limit clamping groove.
Further, in order to enable the sealing film of the reaction cavity 63 of the detection card 6 to correspond to the pointed end 41 of the flow channel 4 of the sample mixing device, the detection card 6 is prevented from being dislocated with the sample mixing device, so that the mixed liquid splashes outwards, the test result is influenced, and the experimental environment is polluted, in some embodiments, the positioning guide rail 52 is arranged on at least one side wall 5 of the clamping opening, and the positioning guide groove matched with the positioning guide rail 52 is arranged at the corresponding position of the detection card 6.
Of course, in other embodiments, a positioning guide groove may be provided on at least one side wall 5 of the card-engaging opening, and a positioning guide rail matched with the positioning guide groove may be provided at a corresponding position on the detection card 6.
Further, in some embodiments of the present invention, a first clamping position and a second clamping position are disposed on the side wall 5 of the clamping opening at intervals along the extending direction of the side wall 5; the detection card 6 is arranged at the second clamping position in a mode of moving relative to the clamping opening; when the detection card 6 is moved from the second clamping position to the first clamping position by an external force, the flow channel 4 of the sample mixing device is in communication with the reaction chamber 63 of the detection card 6.
Specifically, in some embodiments of the present invention, one or both sides of the detection card 6 are respectively provided with a first limit slot 61 and a second limit slot 62 corresponding to the first clamping position and the second clamping position; accordingly, the snap opening comprises: the sliding guide rail is used for providing a sliding path for the detection card 6, and a limiting card protrusion which can be matched with the first limiting card slot and the second limiting card slot is arranged on the sliding guide rail; when the limiting clamping protrusion is matched with the second limiting clamping groove, the detection card 6 is clamped at a second clamping position with the clamping opening; when under the effect of external acting force for spacing protruding the breaking away from the spacing draw-in groove of second, detect card 6 and can follow the guide rail and to being close to 4 directions movements of circulation passageway, and move to spacing protruding position of card when first spacing draw-in groove to when cooperating with spacing protruding phase of card, detect card 6 and joint opening joint in first joint position.
Preferably, in some embodiments of the present invention, the snap opening comprises: a guide rail for providing a sliding path for the detection card 6, and a limit card projection 51 is arranged on the guide rail; correspondingly, one side or two sides of the detection card 6 corresponding to the first clamping position are provided with first limiting clamping grooves which can be matched with the limiting clamping protrusions; the tail end of the limiting clamp protrusion is fixedly connected to a second clamping position on the detection card 6, and a breaking line which is easy to break is arranged at the connection position of the limiting clamp protrusion and the second clamping position;
when the limiting clamp is disconnected from the detection clamp under the action of an external acting force, the detection clamp can move towards the direction close to the flow channel along the guide rail; and when the first limiting clamping groove moves to the position of the limiting clamping protrusion and is matched with the limiting clamping protrusion, the detection card is clamped with the clamping opening at the first clamping position.
In another aspect, provided herein is the use of a microfluidic detection assembly or sample mixing device in antibody detection or antigen detection (e.g., anti-human globulin test, irregular antibody screening test, cross-matching test).
It should be noted that, in this document, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus. Without further limitation, an element defined by the phrase "comprising an … …" does not exclude the presence of other like elements in a process, method, article, or apparatus that comprises the element.
While the present invention has been described with reference to the embodiments shown in the drawings, the present invention is not limited to the embodiments, which are illustrative and not restrictive, and it will be apparent to those skilled in the art that various changes and modifications can be made therein without departing from the spirit and scope of the invention as defined in the appended claims.
Claims (13)
1. A microfluidic detection assembly comprising: the detection card, its characterized in that still includes: a sample mixing device that is insertable into the test card;
wherein the sample mixing device comprises:
a body;
at least one mixing cell spaced within the body for mixing at least two liquid samples, the mixing cell having an inlet at a first side of the body;
the first end of the at least one circulation channel is respectively communicated with the corresponding mixing pool through a micro channel;
when the detection card is plugged on the sample mixing device, at least one flow channel is respectively communicated with the corresponding reaction cavity in the detection card, and when the assembly is under the action of centrifugal force, the mixed liquid in the mixing pool flows into the flow channel through the micro channel and enters the reaction cavity through the flow channel.
2. The assembly of claim 1, wherein the microchannel is a through-hole or a capillary channel.
3. The assembly of claim 1, wherein the second end of the flow channel extends outside the body and is provided with a pointed tip for piercing a sealing membrane on the reaction chamber;
when the test card is inserted into the sample mixing device, the pointed tip pierces the sealing membrane such that at least one of the mixing wells communicates with the corresponding reaction chamber.
4. The assembly of claim 1, wherein the mixing tank comprises a first mixing zone and a second mixing zone in communication, wherein the second mixing zone has a width that gradually decreases in a direction from a first end of the second mixing zone to a second end of the second mixing zone;
and/or the inlet edge of the mixing pool is provided with a blocking structure for preventing the liquid sample from splashing;
and/or the body is sheet-shaped or plate-shaped, and the at least one mixing tank is arranged inside the body at intervals along the width direction of the body.
5. The assembly of claim 4, wherein a flow channel is disposed within the second mixing zone for flow, the flow channel being in communication with the microchannel.
6. The assembly of claim 1, wherein the sample mixing device further comprises: the two clamping openings are formed by side walls extending from two sides of the body and are used for being inserted with the detection card;
when the detection card is pushed into the clamping opening, the flow channel is communicated with the reaction cavity.
7. The assembly of claim 6, wherein the side wall is provided with a limit clamping protrusion, and the detection card is provided with a limit clamping groove matched with the limit clamping protrusion; or, a limit clamping groove is formed in the side wall, a limit clamping protrusion capable of being matched with the limit clamping groove is arranged on the detection card, and when the limit clamping groove is matched with the limit clamping protrusion, the detection card is clamped with the clamping opening;
or the side wall of the clamping opening is provided with a first clamping position and a second clamping position at intervals along the extending direction of the side wall;
the detection card is movably arranged at the second clamping position relative to the clamping opening;
when the detection card moves from the second clamping position to the first clamping position under the action of an external acting force, the flow channel is communicated with a reaction cavity in the detection card;
or, a second clamping position and a first clamping position are arranged on one side or two sides of the detection card at intervals along the direction gradually far away from the mixing pool;
the detection card is movably arranged at the second clamping position relative to the clamping opening;
when under the action of external acting force, the detection card is moved from the second clamping position to the first clamping position, and the circulation channel is communicated with the reaction cavity in the detection card.
8. The assembly of claim 7, wherein one or both sides of the test card are each provided with a stopper protrusion, and accordingly, the card opening comprises: the guide rail is used for providing a moving path for the detection card, and a first limiting clamping groove and a second limiting clamping groove which can be matched with the limiting clamping protrusions are respectively arranged at the first clamping position corresponding to the clamping opening and the second clamping position corresponding to the clamping opening on the guide rail; when the limiting clamping protrusion is matched with the second limiting clamping groove, the detection card and the clamping opening are clamped at the second clamping position of the clamping opening; when the limiting clamping protrusion is separated from the second limiting clamping groove under the action of an external acting force, the detection card can move towards the direction close to the circulation channel along the guide rail, and when the limiting clamping protrusion moves to the first limiting clamping groove and is matched with the first limiting clamping groove, the detection card and the clamping opening are clamped at the first clamping position of the clamping opening;
or, one side or both sides of detection card are provided with a spacing card protruding respectively, correspondingly, the joint opening includes: the guide rail is used for providing a moving path for the detection card, and first limiting clamping grooves which can be matched with the limiting clamping protrusions are respectively arranged at first clamping positions corresponding to the clamping openings on the guide rail; the tail end of the limiting clamping protrusion is fixedly connected with the second clamping position, corresponding to the clamping opening, on the guide rail, and a breaking line which is easy to break is arranged at the joint of the limiting clamping protrusion and the second clamping position of the clamping opening; when the limiting clamping protrusion is disconnected with the guide rail under the action of an external acting force, the detection card can move towards the direction close to the circulation channel along the guide rail, and when the limiting clamping protrusion moves to the first limiting clamping groove and is matched with the first limiting clamping groove, the detection card and the clamping opening are clamped at the first clamping position of the clamping opening;
or one side or two sides of the detection card are respectively provided with a first limit clamping groove and a second limit clamping groove corresponding to the first clamping position and the second clamping position of the detection card; accordingly, the clamping opening comprises: the sliding guide rail is used for providing a sliding path for the detection card, and a limiting card protrusion which can be matched with the first limiting card slot and the second limiting card slot is arranged on the sliding guide rail;
when the limiting clamping protrusion is matched with the second limiting clamping groove, the detection card and the clamping opening are clamped at the second clamping position of the detection card;
when the limiting clamping protrusions are separated from the second limiting clamping grooves under the action of an external acting force, the detection card can move towards the direction close to the circulation channel along the guide rail, and when the first limiting clamping grooves move to the limiting clamping protrusion positions and are matched with the limiting clamping protrusions, the detection card and the clamping opening are clamped at the first clamping position of the detection card;
alternatively, the clamping opening includes: the guide rail is used for providing a sliding path for the detection card, and a limiting card protrusion is arranged on the guide rail; correspondingly, a first limiting clamping groove which can be matched with the limiting clamping protrusion is arranged at one side or two sides of the detection card corresponding to the first clamping position of the detection card; the tail end of the limiting clamp protrusion is fixedly connected to the second clamping position on the detection card, and a breaking line which is easy to break is arranged at the connection position of the limiting clamp protrusion and the second clamping position of the detection card;
when the limiting clamp protrusion is disconnected from the detection clamp under the action of an external acting force, the detection clamp can move towards the direction close to the flow channel along the guide rail; and work as first spacing draw-in groove removes extremely spacing protruding position department of card, and with when spacing protruding phase-match of card, the detection card with joint opening joint in the detection card first joint position.
9. A sample mixing device, comprising:
a body;
at least one mixing cell spaced within the body for mixing at least two liquid samples, the mixing cell having an inlet at a first side of the body;
the first end of the at least one circulation channel is respectively communicated with the corresponding mixing pool through a micro channel.
10. The device of claim 9, wherein the microchannel is a through-hole or a capillary channel;
and/or the second end of the flow channel extends out of the body and is provided with a sharp tail end for puncture;
and/or the body is sheet-shaped or plate-shaped, and the at least one mixing tank is arranged inside the body at intervals along the width direction of the body.
11. The apparatus of claim 9, further comprising: the clamping opening is formed by side walls extending from two sides of the body and is used for being inserted with the detection card;
when the detection card is pushed into the clamping opening, the at least one circulation channel is respectively communicated with the corresponding reaction cavity on the detection card.
12. The device of claim 11, wherein the side wall is provided with a limit card protrusion which can be matched with a limit card slot on the detection card; or
The side wall is provided with a limit clamping groove which can be matched with the limit clamping protrusion on the detection card.
13. Use of a microfluidic detection assembly or sample mixing device according to any one of claims 1-12 for antibody detection or antigen detection.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114324958A (en) * | 2022-03-17 | 2022-04-12 | 天津德祥生物技术有限公司 | Application of sample card and application of sample subassembly |
| CN114324957A (en) * | 2022-03-16 | 2022-04-12 | 天津德祥生物技术有限公司 | Blood type positive and negative shaping sample adding card and sample adding assembly |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20080022027A (en) * | 2006-09-05 | 2008-03-10 | 삼성전자주식회사 | Centrifugal microfluidic device for target protein detection and microfluidic system comprising the same |
| US20080090256A1 (en) * | 2006-10-13 | 2008-04-17 | Ian Gibbons | Reducing optical interference in a fluidic device |
| CN207215840U (en) * | 2017-10-12 | 2018-04-10 | 大连东方雍和生物科技发展有限公司 | A kind of micro-column gel card for Blood grouping |
| CN207440112U (en) * | 2017-10-17 | 2018-06-01 | 天津德祥生物技术有限公司 | A kind of new microtrabeculae blood type card |
| CN112221545A (en) * | 2020-12-10 | 2021-01-15 | 天津德祥生物技术有限公司 | Multi-channel microfluidic sample adding device and application thereof |
| CN112675933A (en) * | 2019-10-18 | 2021-04-20 | 利多(香港)有限公司 | Microfluidic chip for detecting analyte |
-
2021
- 2021-10-18 CN CN202111206908.4A patent/CN113649098B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20080022027A (en) * | 2006-09-05 | 2008-03-10 | 삼성전자주식회사 | Centrifugal microfluidic device for target protein detection and microfluidic system comprising the same |
| US20080090256A1 (en) * | 2006-10-13 | 2008-04-17 | Ian Gibbons | Reducing optical interference in a fluidic device |
| CN207215840U (en) * | 2017-10-12 | 2018-04-10 | 大连东方雍和生物科技发展有限公司 | A kind of micro-column gel card for Blood grouping |
| CN207440112U (en) * | 2017-10-17 | 2018-06-01 | 天津德祥生物技术有限公司 | A kind of new microtrabeculae blood type card |
| CN112675933A (en) * | 2019-10-18 | 2021-04-20 | 利多(香港)有限公司 | Microfluidic chip for detecting analyte |
| CN112221545A (en) * | 2020-12-10 | 2021-01-15 | 天津德祥生物技术有限公司 | Multi-channel microfluidic sample adding device and application thereof |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114324957A (en) * | 2022-03-16 | 2022-04-12 | 天津德祥生物技术有限公司 | Blood type positive and negative shaping sample adding card and sample adding assembly |
| CN114324957B (en) * | 2022-03-16 | 2022-05-20 | 天津德祥生物技术有限公司 | Blood type positive and negative shaping sample adding card and sample adding assembly |
| CN114324958A (en) * | 2022-03-17 | 2022-04-12 | 天津德祥生物技术有限公司 | Application of sample card and application of sample subassembly |
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|---|---|
| CN113649098B (en) | 2022-04-15 |
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