CN112089642A - Collagen cultivation method - Google Patents

Collagen cultivation method Download PDF

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Publication number
CN112089642A
CN112089642A CN202011006468.3A CN202011006468A CN112089642A CN 112089642 A CN112089642 A CN 112089642A CN 202011006468 A CN202011006468 A CN 202011006468A CN 112089642 A CN112089642 A CN 112089642A
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CN
China
Prior art keywords
collagen
implant
implantation
nutrient solution
facial
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
CN202011006468.3A
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Chinese (zh)
Inventor
李举
方珲
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Individual
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Individual
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Publication date
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Priority to CN202011006468.3A priority Critical patent/CN112089642A/en
Publication of CN112089642A publication Critical patent/CN112089642A/en
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    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00Cosmetics or similar toiletry preparations
    • A61K8/18Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/64Proteins; Peptides; Derivatives or degradation products thereof
    • A61K8/65Collagen; Gelatin; Keratin; Derivatives or degradation products thereof
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61QSPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00Preparations for care of the skin
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61QSPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00Preparations for care of the skin
    • A61Q19/08Anti-ageing preparations
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/80Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
    • A61K2800/91Injection

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Dermatology (AREA)
  • Birds (AREA)
  • Epidemiology (AREA)
  • Gerontology & Geriatric Medicine (AREA)
  • Materials For Medical Uses (AREA)

Abstract

The invention provides a method for cultivating collagen, which comprises the steps of uniformly mixing a prepared collagen implant and a normal saline injection according to a proportion, then carrying out facial implantation on the uniformly mixed composite nutrient solution containing the collagen by layers, and then, after 40 days of implanting the collagen, superposing and implanting the collagen composite nutrient solution again according to the facial condition. According to the invention, the collagen is implanted in different layers of the skin effectively by adopting a mode of implanting the collagen in layers and superposition, under the environment of directly supplementing the collagen to the skin, a large amount of fibroblasts are attached and increased around the implanted collagen, the activity of the fibroblasts can be activated, the high-quality collagen can be effectively induced to regenerate, and the collagen regeneration and tissue thickening effects are obvious. When the collagen is degraded, new collagen is remained, so that the aim of facial rejuvenation can be effectively fulfilled.

Description

Collagen cultivation method
Technical Field
The invention relates to the technical field of beauty treatment, in particular to a collagen cultivation method.
Background
Collagen is a main component in human connective tissues, is widely distributed in various tissues of a human body, and mainly plays roles in moisturizing, whitening, preventing wrinkles and resisting aging. But with age the synthesis capacity of fibroblasts decreases. In case of insufficient collagen in the skin, the collagen fibers are cross-linked and solidified, which reduces intercellular mucopolysaccharides, thereby causing skin aging and loss of luster, softness and elasticity. When the skin is aged, dermal fibers are broken, fat is shrunk, secretion of sweat glands and sebaceous glands is reduced, and a series of aging problems such as color spots, wrinkles and looseness can also occur.
Disclosure of Invention
Therefore, based on the above background, the present invention provides a collagen cultivation method, which achieves the effects of delaying the loss rate of collagen in the skin, improving aging cells of the skin, promoting the proliferation of collagen, reducing the appearance of necrotic tissues, and keeping the skin in a young state all the time by implanting collagen implants in a layered and overlapping manner.
The technical scheme provided by the invention is as follows:
a method for collagen cultivation comprises the following steps:
(1) preparing a collagen implant;
(2) uniformly mixing the collagen implant and 0.9% of normal saline injection by using a disposable sterile cock;
(3) carrying out facial implantation on the uniformly mixed compound nutrient solution containing the collagen in the step (2) in layers;
(4) and (4) after 40 days of collagen implantation according to the step (3), performing the operation according to the step (3) again to superpose and implant the collagen composite nutrient solution according to the facial condition.
Further, the volume preparation ratio of the collagen implant and the physiological saline in the step (2) is 1: 4.
Further, the amount of the collagen implant in the step (2) is 1ml, and the amount of the physiological saline is 4 ml.
Further, the implantation tool adopted in the step (3) is a disposable sterile insulin syringe with the implantation levels of the periosteum upper layer, the deep fascia layer, the shallow fascia layer, the subcutaneous tissue and the dermis layer.
The invention has the beneficial effects that:
according to the invention, the collagen is implanted in different layers of the skin effectively by adopting a mode of implanting the collagen in layers and superposition, under the environment of directly supplementing the collagen to the skin, a large amount of fibroblasts are attached and increased around the implanted collagen, the activity of the fibroblasts can be activated, the high-quality collagen can be effectively induced to regenerate, and the collagen regeneration and tissue thickening effects are obvious. When the collagen is degraded, new collagen is remained, so that the aim of facial rejuvenation can be effectively fulfilled.
Detailed Description
The present invention will be described in further detail with reference to specific examples.
Example 1: a method for collagen cultivation comprises the following steps:
(1) 1ml of a collagen implant (Koimei) manufactured by Shuangmei Biotechnology Ltd was prepared, and the specification thereof was 1 ml/piece.
(2) And (3) uniformly mixing the collagen implant prepared in the step (1) and 100ml of 0.9% physiological saline injection by using a disposable sterile stopcock.
(3) Carrying out facial implantation on the uniformly mixed compound nutrient solution containing the collagen in the step (2) in layers by adopting a disposable 1ml sterile insulin syringe; the implanted layers are periosteum upper layer, deep fascia layer, shallow fascia layer, subcutaneous tissue and dermis layer.
(4) And (4) after 40 days of collagen implantation according to the step (3), performing the operation according to the step (3) again to superpose and implant the collagen composite nutrient solution according to the facial condition.
The foregoing descriptions of specific exemplary embodiments of the present invention have been presented for purposes of illustration and description. It is not intended to limit the invention to the precise form disclosed, and obviously many modifications and variations are possible in light of the above teaching. The exemplary embodiments were chosen and described in order to explain certain principles of the invention and its practical application to enable one skilled in the art to make and use various exemplary embodiments of the invention and various alternatives and modifications as are suited to the particular use contemplated. It is intended that the scope of the invention be defined by the claims and their equivalents.

Claims (4)

1. A method for collagen cultivation is characterized by comprising the following steps:
(1) preparing a collagen implant;
(2) uniformly mixing the collagen implant and 0.9% of normal saline injection by using a disposable sterile cock;
(3) carrying out facial implantation on the uniformly mixed compound nutrient solution containing the collagen in the step (2) in layers;
(4) and (4) after 40 days of collagen implantation according to the step (3), performing the operation according to the step (3) again to superpose and implant the collagen composite nutrient solution according to the facial condition.
2. A method of collagen cultivation as claimed in claim 1, wherein the volume ratio of collagen implant and physiological saline in step (2) is 1: 4.
3. A method of collagen cultivation according to claim 2, wherein the amount of collagen implant in step (2) is 1ml and the amount of physiological saline is 4 ml.
4. A method of collagen cultivation according to claim 1, wherein the implantation tool used in step (3) is a disposable 1ml sterile insulin syringe, and the implantation level is periosteum superior layer, deep fascia layer, shallow fascia layer, subcutaneous tissue and dermis layer respectively.
CN202011006468.3A 2020-09-23 2020-09-23 Collagen cultivation method Withdrawn CN112089642A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202011006468.3A CN112089642A (en) 2020-09-23 2020-09-23 Collagen cultivation method

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202011006468.3A CN112089642A (en) 2020-09-23 2020-09-23 Collagen cultivation method

Publications (1)

Publication Number Publication Date
CN112089642A true CN112089642A (en) 2020-12-18

Family

ID=73755886

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202011006468.3A Withdrawn CN112089642A (en) 2020-09-23 2020-09-23 Collagen cultivation method

Country Status (1)

Country Link
CN (1) CN112089642A (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
LU501530B1 (en) * 2022-02-21 2023-08-21 Ju Li A method for preparing and implanting collagen

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
LU501530B1 (en) * 2022-02-21 2023-08-21 Ju Li A method for preparing and implanting collagen

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Application publication date: 20201218