CN112088904A - Preparation method and application of paris polyphylla extractive solution - Google Patents
Preparation method and application of paris polyphylla extractive solution Download PDFInfo
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- CN112088904A CN112088904A CN202011138188.8A CN202011138188A CN112088904A CN 112088904 A CN112088904 A CN 112088904A CN 202011138188 A CN202011138188 A CN 202011138188A CN 112088904 A CN112088904 A CN 112088904A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/04—Pteridophyta [fern allies]; Filicophyta [ferns]
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Abstract
The invention relates to the technical field of active ingredient extraction, and particularly relates to a preparation method of a paris polyphylla extractive solution, which comprises the following steps: taking a proper amount of rhizome of paris polyphylla, cleaning, slicing, crushing to 200 meshes, placing in an enzymolysis tank, adding 6 times of deionized water, placing in a constant-temperature water bath shaking table at 60-80 ℃, sequentially adding fungal protease, cellulase, pectinase and alpha-amylase, performing enzymolysis for 1h respectively, homogenizing for 20min under the pressure of 200-400 MPa, inactivating enzyme, placing in a high-pressure reaction kettle, adding a proper amount of deionized water, and performing subcritical water extraction for 20-30 min to obtain an extracting solution. According to the invention, the paris polyphylla extracting solution is prepared by adopting a method of high-pressure homogenization treatment, subcritical water extraction and enzymolysis coupling, so that the active ingredients of paris polyphylla powder can be rapidly separated out, the extraction time is shortened, the extraction efficiency is improved, and no waste residue is generated.
Description
Technical Field
The invention relates to the technical field of extraction of active ingredients of traditional Chinese medicinal materials, and particularly relates to a preparation method and application of a paris polyphylla extracting solution.
Background
The main chemical components of paris polyphylla comprise steroids, flavonoid glycoside, various amino acids, a small amount of alkaloid, phenols, tannin, trace elements and the like, have the effects of stopping bleeding, regulating immunity, easing pain, resisting bacteria, diminishing inflammation, clearing heat, resisting tumors and the like, have obvious effects on treating snake venom, epidemic encephalitis B, stomachache, appendicitis, tuberculous lymphadenitis, tonsillitis, parotitis, mastitis and the like, and are applied to the pharmaceutical industry. For example, the invention patents "201810088740.3 a pharmaceutical composition for treating acute mastitis and a preparation method thereof", "201810005604.3 a skin antibacterial drug", "201810005604.3 a drug for treating thrombus-type external hemorrhoid and a preparation method thereof", and the like, and documents of "national botany of paris polyphylla research" published by ripples and the like, and "analysis of paris polyphylla volatile oil components and antibacterial activity thereof" published by artemisia anomala and the like. Although Paris polyphylla has been widely applied in the pharmaceutical industry, the utilization of Paris polyphylla in the field of botanical pesticides is yet to be developed.
At present, the preparation method of the paris polyphylla extracting solution mainly comprises an extraction method, a water boiling method, an ultrasonic extraction method and the like, and generally has the defects of low extraction efficiency and more waste residues.
Disclosure of Invention
In order to solve the problems, the invention provides a preparation method and application of a paris polyphylla extracting solution, which can obviously improve the extraction efficiency of active ingredients and can not generate waste residues.
In order to achieve the purpose, the invention adopts the technical scheme that:
a preparation method of paris polyphylla extractive solution comprises the following steps:
s1, taking a proper amount of rhizome of paris polyphylla, cleaning, slicing, and crushing to 200 meshes to obtain paris polyphylla powder;
s2, placing the obtained paris polyphylla powder in an enzymolysis tank, adding 6 times of deionized water, placing the paris polyphylla powder in a constant-temperature water bath shaking table at the temperature of 60-80 ℃, sequentially adding fungal protease, cellulase, pectinase and alpha-amylase, performing enzymolysis for 1h respectively, homogenizing for 20min under the pressure of 200-400 MPa, and inactivating enzymes to obtain an enzyme inactivation solution;
s3, placing the enzyme deactivation solution into a high-pressure reaction kettle, adding a proper amount of deionized water, and performing subcritical water extraction treatment for 20-30 min at 100-160 ℃ to obtain an extracting solution.
Further, in the step S2, the mass ratio of the fungal protease, the cellulase, the pectinase, the alpha-amylase and the paris polyphylla pollen is 1 to 10.
Further, in the step S2, the flow rate of the homogenization treatment is 4 to 6L/h, and the temperature of the enzymatic hydrolysate is between 10 and 20 ℃.
Further, in step S2, after placing the mixture in a constant temperature water bath shaker at 60-80 ℃, ultrasonically dispersing for 15min, and sequentially adding fungal protease, cellulase, pectinase and alpha-amylase.
Furthermore, during ultrasonic dispersion, the power is 50-200W, and the frequency is 30-90 Hz.
Further, in the step S3, the feed-liquid ratio of subcritical water extraction is 10-15 mL/g.
The invention also provides a new application of the paris polyphylla extractive solution, and the new application is used for preparing the plant source antiviral agent.
The invention has the following beneficial effects:
the paris polyphylla extract is prepared by adopting a method of coupling high-pressure homogenization treatment, subcritical water extraction and enzymolysis, so that active ingredients of paris polyphylla powder can be quickly separated out, the extraction time is shortened, the extraction efficiency is improved, and no waste residue is generated.
Detailed Description
The present invention will be described in detail with reference to specific examples. The following examples will assist those skilled in the art in further understanding the invention, but are not intended to limit the invention in any way. It should be noted that variations and modifications can be made by persons skilled in the art without departing from the spirit of the invention. All falling within the scope of the present invention.
Example 1
A preparation method of paris polyphylla extractive solution comprises the following steps:
s1, taking a proper amount of rhizome of paris polyphylla, cleaning, slicing, and crushing to 200 meshes to obtain paris polyphylla powder;
s2, placing the obtained paris polyphylla powder in an enzymolysis tank, adding 6 times of deionized water, placing the paris polyphylla powder in a constant-temperature water bath shaking table at 60-80 ℃, ultrasonically dispersing for 15min, and then sequentially adding fungal protease, cellulase, pectinase and alpha-amylase, wherein the mass ratio of the fungal protease, the cellulase, the pectinase, the alpha-amylase to the paris polyphylla powder is 1: 10, after 1h of enzymolysis, homogenizing under the pressure of 200MPa for 20min, and inactivating enzymes to obtain an enzyme inactivation solution;
s3, placing the enzyme-inactivating solution in a high-pressure reaction kettle, adding a proper amount of deionized water, and performing subcritical water extraction treatment at 100 ℃ for 30min to obtain an extracting solution.
In this example, the flow rate of homogenization treatment was 4L/h, the temperature of the enzymatic hydrolysate was 10 ℃, the power was 150W, the frequency was 60Hz, and the ratio of subcritical water extraction to feed solution was 12mL/g during ultrasonic dispersion.
Example 2
A preparation method of paris polyphylla extractive solution comprises the following steps:
s1, taking a proper amount of rhizome of paris polyphylla, cleaning, slicing, and crushing to 200 meshes to obtain paris polyphylla powder;
s2, placing the obtained paris polyphylla powder in an enzymolysis tank, adding 6 times of deionized water, placing the paris polyphylla powder in a constant-temperature water bath shaking table at 60-80 ℃, ultrasonically dispersing for 15min, and then sequentially adding fungal protease, cellulase, pectinase and alpha-amylase, wherein the mass ratio of the fungal protease, the cellulase, the pectinase, the alpha-amylase to the paris polyphylla powder is 1: 10, after 1h of enzymolysis, homogenizing under the pressure of 400MPa for 20min, and inactivating enzymes to obtain an enzyme inactivation solution;
s3, placing the enzyme-inactivating solution in a high-pressure reaction kettle, adding a proper amount of deionized water, and performing subcritical water extraction treatment at 160 ℃ for 20min to obtain an extracting solution.
In this example, the flow rate of homogenization treatment was 6L/h, the temperature of the enzymatic hydrolysate was 20 ℃, the power was 120W, the frequency was 60Hz, and the ratio of subcritical water extraction to feed solution was 13mL/g during ultrasonic dispersion.
Example 3
A preparation method of paris polyphylla extractive solution comprises the following steps:
s1, taking a proper amount of rhizome of paris polyphylla, cleaning, slicing, and crushing to 200 meshes to obtain paris polyphylla powder;
s2, placing the obtained paris polyphylla powder in an enzymolysis tank, adding 6 times of deionized water, placing the paris polyphylla powder in a constant-temperature water bath shaking table at 60-80 ℃, ultrasonically dispersing for 15min, and then sequentially adding fungal protease, cellulase, pectinase and alpha-amylase, wherein the mass ratio of the fungal protease, the cellulase, the pectinase, the alpha-amylase to the paris polyphylla powder is 1: 10, after 1h of enzymolysis, homogenizing under the pressure of 300MPa for 20min, and inactivating enzymes to obtain an enzyme inactivation solution;
s3, placing the enzyme-inactivating solution in a high-pressure reaction kettle, adding a proper amount of deionized water, and performing subcritical water extraction treatment at 130 ℃ for 25min to obtain an extracting solution.
In the embodiment, the flow rate of homogenization treatment is 5L/h, the temperature of the zymolyte is 15 ℃, the power is 120W, the frequency is 60Hz, and the ratio of subcritical water extraction to feed liquid is 15mL/g during ultrasonic dispersion.
Proved by verification, compared with an alcohol extraction method, a water boiling method, a water ultrasonic extraction method and an alcohol ultrasonic extraction method, the extraction method can obviously reduce the extraction time and does not generate waste residues under the same target product amount.
The foregoing description of specific embodiments of the present invention has been presented. It is to be understood that the present invention is not limited to the specific embodiments described above, and that various changes or modifications may be made by one skilled in the art within the scope of the appended claims without departing from the spirit of the invention. The embodiments and features of the embodiments of the present application may be combined with each other arbitrarily without conflict.
Claims (7)
1. A preparation method of paris polyphylla extract is characterized by comprising the following steps: the method comprises the following steps:
s1, taking a proper amount of rhizome of paris polyphylla, cleaning, slicing, and crushing to 200 meshes to obtain paris polyphylla powder;
s2, placing the obtained paris polyphylla powder in an enzymolysis tank, adding 6 times of deionized water, placing the paris polyphylla powder in a constant-temperature water bath shaking table at the temperature of 60-80 ℃, sequentially adding fungal protease, cellulase, pectinase and alpha-amylase, performing enzymolysis for 1h respectively, homogenizing for 20min under the pressure of 200-400 MPa, and inactivating enzymes to obtain an enzyme inactivation solution;
s3, placing the enzyme deactivation solution into a high-pressure reaction kettle, adding a proper amount of deionized water, and performing subcritical water extraction treatment for 20-30 min at 100-160 ℃ to obtain an extracting solution.
2. The method for preparing paris polyphylla extractive solution as claimed in claim 1, wherein the method comprises the following steps: in the step S2, the mass ratio of the fungal protease, the cellulase, the pectinase, the alpha-amylase and the paris polyphylla pollen is 1 to 10.
3. The method for preparing paris polyphylla extractive solution as claimed in claim 1, wherein the method comprises the following steps: in the step S2, the flow rate of the homogenization treatment is 4-6L/h, and the temperature of the enzymolysis solution is 10-20 ℃.
4. The method for preparing paris polyphylla extractive solution as claimed in claim 1, wherein the method comprises the following steps: in step S2, placing the mixture in a constant-temperature water bath shaking table at the temperature of 60-80 ℃, ultrasonically dispersing for 15min, and then sequentially adding fungal protease, cellulase, pectinase and alpha-amylase.
5. The method for preparing paris polyphylla extractive solution as claimed in claim 4, wherein the method comprises the following steps: and during ultrasonic dispersion, the power is 50-200W, and the frequency is 30-90 Hz.
6. The method for preparing paris polyphylla extractive solution as claimed in claim 1, wherein the method comprises the following steps: in the step S3, the feed-liquid ratio of subcritical water extraction is 10-15 mL/g.
7. The use of the Paris polyphylla extract prepared by the preparation method according to any one of claims 1 to 6, wherein: can be used for preparing plant-derived antiviral agent.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN113730317A (en) * | 2021-09-14 | 2021-12-03 | 浙江宜格企业管理集团有限公司 | Method for extracting effective ingredients for cosmetics from paris polyphylla |
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Application publication date: 20201218 |