CN112080566A - 基于高通量测序法的甲状腺癌检测产品及用途 - Google Patents
基于高通量测序法的甲状腺癌检测产品及用途 Download PDFInfo
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- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
- C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6858—Allele-specific amplification
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- G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
- G16B20/00—ICT specially adapted for functional genomics or proteomics, e.g. genotype-phenotype associations
- G16B20/20—Allele or variant detection, e.g. single nucleotide polymorphism [SNP] detection
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- G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
- G16B25/00—ICT specially adapted for hybridisation; ICT specially adapted for gene or protein expression
- G16B25/20—Polymerase chain reaction [PCR]; Primer or probe design; Probe optimisation
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- G—PHYSICS
- G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
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- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/16—Primer sets for multiplex assays
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Abstract
Description
主要组成成分 | 规格 |
文库引物混合液 | 192μL/管,1管 |
HIFI PCR混合液 | 384μL/管,1管 |
FuPa试剂 | 192μL/管,1管 |
连接缓冲液 | 384μL/管,1管 |
DNA连接酶 | 192μL/管,1管 |
HIFI酶混合液 | 1600μL/管,3管 |
DNA PCR引物混合液 | 192μL/管,1管 |
RNA PCR引物混合液 | 192μL/管,1管 |
洗脱缓冲液 | 12mL/管,1管 |
逆转录酶 | 48μL/管,1管 |
逆转录酶缓冲液 | 96μL/管,1管 |
接头 | 192μL/管,1管 |
标签N(N=1,2…96) | 2μL/管,96管 |
文库纯化磁珠 | 960μL/管,16管 |
Claims (14)
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CN202010864045.9A CN112080566B (zh) | 2020-08-25 | 2020-08-25 | 基于高通量测序法的甲状腺癌检测产品及用途 |
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CN202010864045.9A CN112080566B (zh) | 2020-08-25 | 2020-08-25 | 基于高通量测序法的甲状腺癌检测产品及用途 |
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CN112080566A true CN112080566A (zh) | 2020-12-15 |
CN112080566B CN112080566B (zh) | 2021-08-17 |
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Citations (7)
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WO2017095487A1 (en) * | 2015-12-01 | 2017-06-08 | Seracare Life Sciences, Inc. | Multiplex cellular reference materials |
CN106912197A (zh) * | 2011-04-28 | 2017-06-30 | 生命技术公司 | 用于多重pcr的方法和组合物 |
CN107164513A (zh) * | 2017-06-22 | 2017-09-15 | 上海鹍远生物技术有限公司 | 基于高通量测序法的人类多基因突变检测试剂盒 |
CN108699553A (zh) * | 2015-12-31 | 2018-10-23 | 奎斯特诊断投资有限责任公司 | 用于筛选甲状腺癌中突变的组合物和方法 |
CN109371139A (zh) * | 2018-12-29 | 2019-02-22 | 杭州迪安医学检验中心有限公司 | 一种基于高通量测序技术用于检测甲状腺癌致病相关基因变异的引物及其应用 |
CN109811055A (zh) * | 2019-01-08 | 2019-05-28 | 广州金域医学检验中心有限公司 | 肉瘤融合基因检测试剂盒及系统 |
CN110878358A (zh) * | 2019-12-19 | 2020-03-13 | 上海宝藤生物医药科技股份有限公司 | 一组甲状腺癌标志物及其应用 |
-
2020
- 2020-08-25 CN CN202010864045.9A patent/CN112080566B/zh active Active
Patent Citations (7)
Publication number | Priority date | Publication date | Assignee | Title |
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CN106912197A (zh) * | 2011-04-28 | 2017-06-30 | 生命技术公司 | 用于多重pcr的方法和组合物 |
WO2017095487A1 (en) * | 2015-12-01 | 2017-06-08 | Seracare Life Sciences, Inc. | Multiplex cellular reference materials |
CN108699553A (zh) * | 2015-12-31 | 2018-10-23 | 奎斯特诊断投资有限责任公司 | 用于筛选甲状腺癌中突变的组合物和方法 |
CN107164513A (zh) * | 2017-06-22 | 2017-09-15 | 上海鹍远生物技术有限公司 | 基于高通量测序法的人类多基因突变检测试剂盒 |
CN109371139A (zh) * | 2018-12-29 | 2019-02-22 | 杭州迪安医学检验中心有限公司 | 一种基于高通量测序技术用于检测甲状腺癌致病相关基因变异的引物及其应用 |
CN109811055A (zh) * | 2019-01-08 | 2019-05-28 | 广州金域医学检验中心有限公司 | 肉瘤融合基因检测试剂盒及系统 |
CN110878358A (zh) * | 2019-12-19 | 2020-03-13 | 上海宝藤生物医药科技股份有限公司 | 一组甲状腺癌标志物及其应用 |
Non-Patent Citations (6)
Title |
---|
BAIRONG SHEN: "《Bioinformatics for Diagnosis, Prognosis and Treatment of Complex Diseases》", 31 December 2013, SPRINGER * |
LAMIS YEHIA等: "Thyroglobulin in Metastatic Thyroid Cancer: Culprit or Red Herring?", 《AMERICAN SOCIETY OF HUMAN GENETICS》 * |
MICHELE SIMBOLO等: "High-throughput mutation profiling improves diagnostic stratification of sporadic medullary thyroid carcinomas", 《VIRCHOWS ARCH》 * |
NIKIFOROVA MN等: "Targeted next-generation sequencing panel (ThyroSeq) for detection of mutations in thyroid cancer", 《ENDOCRINOL METAB. 》 * |
SYKOROVA等: "Search for new genetic biomarkers in poorly differentiated and anaplastic thyroid carcinomas using next generation sequencing", 《ANTICANCER RES.》 * |
YOON JIN CHA等: "Next-generation sequencing in thyroid cancer", 《J TRANSL MED》 * |
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Address after: 197 Ruijin 2nd Road, Huangpu District, Shanghai 200025 Patentee after: RUI JIN HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG University SCHOOL OF MEDICINE Patentee after: Shanghai Fuyuan Biotechnology Co.,Ltd. Patentee after: Jiangsu Fuyuan Biotechnology Co.,Ltd. Address before: 197 Ruijin 2nd Road, Huangpu District, Shanghai 200025 Patentee before: RUI JIN HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG University SCHOOL OF MEDICINE Patentee before: SINGLERA GENOMICS (SHANGHAI) Ltd. Patentee before: Jiangsu Fuyuan Biotechnology Co.,Ltd. |
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Address after: 197 Ruijin 2nd Road, Huangpu District, Shanghai 200025 Patentee after: RUI JIN HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG University SCHOOL OF MEDICINE Patentee after: Jiangsu Huayuan Biotechnology Co.,Ltd. Patentee after: Jiangsu Fuyuan Biotechnology Co.,Ltd. Address before: 197 Ruijin 2nd Road, Huangpu District, Shanghai 200025 Patentee before: RUI JIN HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG University SCHOOL OF MEDICINE Patentee before: Shanghai Fuyuan Biotechnology Co.,Ltd. Patentee before: Jiangsu Fuyuan Biotechnology Co.,Ltd. |