CN112057359B - Deep-sea pinus hirsuta moisturizing composition and preparation method and application thereof - Google Patents

Abstract

The application relates to the field of cosmetics, and particularly discloses a deep sea pinus hirsutus moisturizing composition which comprises a deep sea pinus hirsutus moisturizing essence, a deep sea pinus hirsutus moisturizing skin base solution and a deep sea pinus hirsutus multi-dimensional moisturizing cream; the deep-sea pinus hirsuta moisturizing muscle base solution is prepared from the following raw materials in parts by weight: water, butylene glycol, glycerol, betaine, hydrolyzed prunus humilis extract, nicotinamide, glycyrrhiza glabra extract, purslane extract, beta-glucan, modified EGCG, centella asiatica extract, alpine leontopodium floral leaf extract, lilium candidum extract, codium tomentosum extract, sodium hyaluronate, disodium EDTA, 1, 2-hexanediol; has the advantages of improving the stability and the compatibility of EGCG, thereby improving the moisturizing effect of cosmetics; in addition, a preparation method of the deep-sea Ardisia tomentosa moisturizing composition and an application method of the deep-sea Ardisia tomentosa moisturizing composition are also provided.

Description

Deep-sea pinus hirsuta moisturizing composition and preparation method and application thereof

Technical Field

The application relates to the field of cosmetics, in particular to a deep sea pinus tomentosa moisturizing composition and a preparation method and application thereof.

Background

In recent years, people are more and more aware of the importance of skin moisturizing, and with the development of cosmetic science and skin physiology, the key to find whether the skin is fine, plump and bright is the water content of the skin. The stratum corneum, which is the top layer of the skin, has water-absorbing and barrier functions, and amino acids, lactate, saccharides, and the like contained in the stratum corneum maintain a certain water content in the stratum corneum to maintain skin moisturization. Lack of water in the skin can cause the skin to become dry, non-moist, taut, with the long-term consequences of skin aging, wrinkling, mottling, and becoming rough, dull, and inelastic. Because the plant active ingredients have the characteristics of strong efficacy and small side effect, more and more moisturizing products mainly comprising the plant active ingredients appear.

The Pinus tomentosa belongs to Pinonaceae, is a kind of algae growing in intertidal zone, is a family of Chlorophyta, and its green algae body is unicellular, colony-growing, filament or thallus plant. The extract of Pinus hirsuta contains L-citrullinyl-L-arginine and galactosyl glycerol as main ingredients, and can promote synthesis of epidermal cell aquaporin, thereby enhancing water circulation and skin barrier function of skin.

The catechin compound EGCG with the strongest biological activity in the green tea extract endows the cosmetics with multiple effects of delaying photoaging, whitening, anti-allergy, moisturizing and the like, so that the green tea extract or EGCG monomer is called as the most commonly used cosmetic additive. However, the EGCG has the problems of poor stability, poor compatibility, color change, low transdermal absorption utilization rate and the like during application, and the application of the EGCG in cosmetics is also limited.

Disclosure of Invention

In order to improve the stability and compatibility of EGCG so as to improve the moisturizing effect of cosmetics, the first purpose of the application is to provide a deep-sea Arthodium hirsutum moisturizing composition.

The second purpose of the application is to provide a preparation method of the deep-sea Arthodium hirsutum moisturizing composition.

The third purpose of the application is to provide an application method of the deep sea hirsutella himalaica moisturizing composition.

The first purpose provided by the application is to provide a deep-sea Arthodium hirsutum moisturizing composition, which adopts the following technical scheme:

a deep sea Ardisia tomentosa moisturizing composition comprises a deep sea Ardisia tomentosa moisturizing essence, a deep sea Ardisia tomentosa moisturizing skin base solution and a deep sea Ardisia tomentosa multi-vitamin moisturizing cream;

the deep-sea pinus hirsuta moisturizing muscle base solution is prepared from the following raw materials in parts by weight: 90-100 parts of water, 1.4-1.6 parts of butanediol, 2.1-2.3 parts of glycerol, 0.04-0.06 part of betaine, 0.07-0.09 part of hydrolyzed European plum extract, 0.10-0.12 part of nicotinamide, 0.04-0.08 part of glabrous greenbrier rhizome extract, 0.05-0.09 part of purslane extract, 0.14-0.16 part of beta-glucan, 2.3-2.7 parts of modified EGCG, 0.06-0.10 part of centella asiatica extract, 0.07-0.09 part of alpine edelweiss flower leaf extract, 0.10-0.12 part of white lily flower extract, 1.2-1.4 parts of pinus tomentosa extract, 0.01-0.03 part of sodium hyaluronate, 1.8-2.2 parts of EDTA disodium and 1.4-1.8 parts of 1, 2-hexanediol;

wherein, the modified EGCG is obtained by the reaction of palmitoyl pentapeptide-4, maleic anhydride and EGCG.

By adopting the technical scheme, the modified EGCG is prepared by the reaction of the palmitoyl pentapeptide-4, the maleic anhydride and the EGCG, the molecular structure of the EGCG has 3 benzene ring structures, the benzene ring structure of the modified EGCG and amino, carbonyl and other groups on the carbon chain of the palmitoyl pentapeptide-4 are under the electron cloud effect, so that the long carbon chain of the palmitoyl pentapeptide-4 is attached to the molecular surface of the EGCG to form an embedded structure, the EGCG has the effects of difficult decomposition and deterioration, the palmitoyl pentapeptide-4 can penetrate through dermis to increase collagen, the skin aging process is reversed by reconstruction from inside to outside, and the palmitoyl pentapeptide-4 is combined with the moisturizing effect of the EGCG to achieve the states of better moisturizing, transparent and youthful skin, and more plant extracts are contained in the formula, so that the extracts are easy to be absorbed by the skin and are difficult to generate the phenomena of allergy and the like, particularly the Pinus palustris extract, the moisturizing effect of the composition and the modified EGCG achieve a synergistic effect, the skin can firmly lock water, and the excellent moisturizing effect is achieved by matching the deep sea pinus tomentosa water-locking essence and the deep sea pinus tomentosa multi-vitamin water-replenishing cream.

Preferably, the preparation steps of the modified EGCG are as follows according to parts by weight:

1) 1) adding 4.5-5.0 parts of EGCG into 20-25 parts of THF, controlling the temperature at 30-35 ℃, stirring until the EGCG is dissolved, then adding 1.0-1.2 parts of maleic anhydride, after the addition is finished, continuing stirring for reaction for 1-2h, and concentrating to obtain a solid A;

2) dissolving 8-9 parts of palmitoyl pentapeptide-4 in 15-20 parts of THF (tetrahydrofuran), and uniformly stirring to obtain a palmitoyl pentapeptide-4 solution;

3) adding the solid A and 3.3-4.0 parts of carbonyl diimidazole into 20-25 parts of THF, uniformly stirring, controlling the temperature at 6-10 ℃, then dropwise adding the palmitoyl pentapeptide-4 solution at the speed of 2-3mL/min, continuously stirring for reacting for 2-3h after dropwise adding, and performing rotary evaporation to obtain a solid B;

4) adding the solid B into 40-50 parts of water, controlling the temperature to be 35-40 ℃, uniformly stirring, filtering to obtain filtrate, concentrating to obtain solid C, and drying to obtain the modified EGCG.

By adopting the technical scheme, hydroxyl on EGCG attacks carbonyl carbon on maleic anhydride, the formed substance simultaneously has ester group and carboxylic acid group, and then the carboxylic acid group reacts with amino on palmitoyl pentapeptide-4 under the action of carbonyl diimidazole to obtain the modified EGCG.

Preferably, the maleic anhydride is added in 3-5 times in the step 1), and the time interval between two adjacent times is 20-30 min.

By adopting the technical scheme, because the EGCG contains a plurality of hydroxyl groups, when the maleic anhydride is added in several times, the EGCG is in an excessive state relative to the mixed solution, and the one-to-one reaction of the EGCG and the maleic anhydride is facilitated.

Preferably, the drying in the step 4) is performed by vacuum drying, the drying temperature is 40-45 ℃, and the drying time is 3-4 h.

By adopting the technical scheme, the vacuum drying can be carried out at a higher drying speed under the condition of lower temperature.

Preferably, the deep-sea pinus hirsuta water-locking essence is prepared from the following raw materials in parts by weight: 90-100 parts of water, 1.8-2.2 parts of propylene glycol, 10-14 parts of glycerol, 0.08-0.12 part of Ascophyllum nodosum extract, 0.15-0.20 part of rhodococcus extract, 0.18-0.22 part of brown algae extract, 0.08-0.10 part of Laminaria digitata extract, 0.01-0.03 part of Chondrus crispus extract, 1.2-1.4 parts of Pinus lansidaensis extract, 0.16-0.20 part of allantoin, 0.14-0.16 part of carbomer, 1.2-1.4 parts of xanthan gum, 0.8-1.2 parts of 1, 2-hexanediol and 1.0-1.4 parts of ethylhexylglycerol.

By adopting the technical scheme, the essence is easily absorbed by the skin by adding various plant moisturizing components, so that the basal layer of the skin can keep the moisture supplement.

Preferably, the deep sea pinus hirsuta multi-vitamin water replenishing cream is prepared from the following raw materials in parts by weight: 40-50 parts of water, 8-12 parts of butanediol, 0.8-1.0 part of sodium hyaluronate, 1.0-1.4 parts of nicotinamide, 0.18-0.22 part of carbomer, 0.3-0.5 part of jojoba seed oil, 0.09-0.11 part of tocopherol acetate, 0.05-0.07 part of caprylic glycerate, 0.04-0.08 part of glycerol stearate, 0.12-0.14 part of caffeine, 0.09-0.11 part of Ascophyllum nodosum extract, 0.02-0.04 part of Porphyromyces lachnophora extract, 0.05-0.07 part of brown algae extract, 1.6-1.8 parts of Pinus tomentosa extract, 0.01-0.03 part of Laminaria digitata extract, 0.02-0.04 part of Chondrus crispus extract and 2-4 parts of 1, 2-hexanediol.

Through adopting above-mentioned technical scheme, through adding multiple plant moisture retention composition, be difficult for producing the anaphylaxis phenomenon, the effect of moisturizing is better, reaches better lock water effect, makes the difficult loss of moisture.

Preferably, the preparation method of the deep-sea pinus hirsuta moisturizing muscle base solution comprises the following steps: adding butanediol, glycerol, betaine, nicotinamide, beta-glucan, sodium hyaluronate, EDTA disodium and 1, 2-hexanediol into water, stirring until the materials are dissolved, then adding hydrolyzed European plum extract, Glycyrrhiza glabra extract, purslane extract, modified EGCG, centella asiatica extract, Hibiscus alpina flower leaf extract, lilium albo-marginatum flower extract and Pinus tomentosa extract, and uniformly stirring to obtain the deep-sea Pinus tomentosa moisturizing foundation solution.

By adopting the technical scheme, the deep-sea pinus hirsuta moisturizing skin base solution can be prepared by dissolving the raw materials in water.

Preferably, the preparation method of the deep sea pinus hirsuta water-locking essence comprises the following steps:

adding propylene glycol, glycerol, 1, 2-hexanediol, allantoin and ethylhexylglycerin into water, stirring until the propylene glycol, the glycerol, the 1, 2-hexanediol, the allantoin and the ethylhexylglycerin are dissolved, then adding the Ascophyllum nodosum extract, the Rhodococcus rhodochrous extract, the brown algae extract, the Laminaria digitata extract, the Chondrus crispus extract, the Pinus hirsuta extract, the carbomer and the xanthan gum, and stirring until the Podocus hirsutus linn water-locking essence is dissolved, so as to obtain the deep-sea Pinus hirsutus linn water-locking essence.

By adopting the technical scheme, the deep-sea pinus tomentosa water-locking essence can be prepared by dissolving the raw materials in water.

Preferably, the deep sea pinus hirsuta multi-vitamin water replenishing cream comprises the following preparation steps:

adding butanediol, sodium hyaluronate, nicotinamide and 1, 2-hexanediol into water, stirring until the butanediol, sodium hyaluronate, nicotinamide and 1, 2-hexanediol are dissolved, then adding carbomer, jojoba seed oil, tocopherol acetate, caprylic glycerate, glyceryl stearate, a phyllocladium extract, caffeine, a porphyridium extract, a brown algae extract, a codium tomentosum extract, a laminaria key extract and a carrageen crispatus extract, and uniformly stirring to obtain the deep sea codium tomentosum multi-vitamin water replenishing cream.

An application method of a deep-sea pinus hirsuta moisturizing composition comprises the following steps:

1) after skin cleaning, 1.0-1.5mL of deep sea pinus hirsuta water locking essence is smeared on the face and is gently massaged until absorption;

2) after the step 1), 1.0-1.5mL of deep-sea Aronaea tomentosa moisturizing skin base solution is smeared on the face and is gently massaged until absorption;

3) after the step 2), 0.5-0.7mL of deep sea Ardisia tomentosa multi-vitamin water replenishing cream is smeared on the face and is gently massaged until absorption.

By adopting the technical scheme, the deep-sea pinus tomentosa moisturizing essence is smeared firstly, then the deep-sea pinus tomentosa moisturizing skin base solution is smeared, and finally the deep-sea pinus tomentosa multi-dimensional moisturizing cream is smeared, so that a good moisturizing effect can be achieved.

In summary, the present application has the following beneficial effects:

1. after the deep-sea pinus hirsuta moisturizing composition is applied, the hydration rate can still reach over 115.6% after 6 hours, and the moisturizing effect is excellent.

2. According to the application, EGCG is modified through palmitoyl pentapeptide-4, the stability of EGCG is greatly improved, the application range of the EGCG is enlarged, the EGCG can achieve a good application effect, and the moisturizing effect is good.

Detailed Description

The present application will be described in further detail with reference to examples.

Raw materials

EGCG: the manufacturer is Chengdui Dai Biotechnology limited company, the content is 98%;

extract of Ascophyllum nodosum: the manufacturer is a natural biological product of Shanxi Yanling, and the product number is SXHPYZTQW;

the Arthodium hirsutum extract is obtained by concentrating Butylene glycol extractive solution of Arthodium hirsutum;

porphyridium extract: the manufacturer is Shanxi Hana Biotechnology Co., Ltd;

brown algae extract: the manufacturer is Volterus Biotech, Lanzhou;

laminaria angustata extract: the manufacturer is Euppen technology Limited in Shenzhen;

chondrus crispus extract: the manufacturer is the Xian Michel Biotech Co., Ltd;

the pinus hirsuta raw material: the manufacturer is Hangzhou European technology company;

hydrolyzing European plums: the manufacturer is Ningxia vanilla biotech Co., Ltd;

glycyrrhiza glabra extract: the manufacturer is Gansu Probiotics and auspicious biotechnology limited company;

and (3) purslane extract: west anwanfang biotechnology limited;

alpine leontopodium flower and leaf extract: the manufacturer is Guangzhou Tio elegant Biotech limited;

lily extract: the manufacturer is Nanjing Dow Biotech limited;

jojoba seed oil: the manufacturer is Shanghai Huien International trade company;

caffeine: the manufacturer is Zhengzhou Chengwang chemical industry Co.

Preparation example

Preparation example 1

A modified EGCG is prepared by the following steps:

1) adding 4.5kg of EGCG into 20kg of THF, controlling the temperature at 30 ℃, stirring until the EGCG is dissolved, then adding 1.0kg of maleic anhydride, adding the maleic anhydride for 3 times in equal amount, wherein the time interval between every two adjacent times is 30min, after the addition is finished, continuously stirring for reacting for 1h, and performing rotary evaporation to obtain a solid A;

2) dissolving 8kg of palmitoyl pentapeptide-4 in 15kg of THF, and uniformly stirring to obtain a palmitoyl pentapeptide-4 solution;

3) adding the solid A and 3.3kg of carbonyl diimidazole into 20kg of THF, uniformly stirring, controlling the temperature at 6 ℃, then dropwise adding the palmitoyl pentapeptide-4 solution at the dropwise adding speed of 2mL/min, continuously stirring for reacting for 2 hours after dropwise adding, and rotationally evaporating to obtain a solid B;

4) adding the solid B into 40kg of water, controlling the temperature to be 35 ℃, uniformly stirring, filtering to obtain filtrate, performing rotary evaporation on the filtrate to obtain solid C, and performing vacuum drying on the solid C for 4 hours at the temperature of 40 ℃ to obtain the modified EGCG.

Preparation example 2

A modified EGCG is prepared by the following steps:

1) adding 4.7kg of EGCG into 23kg of THF, controlling the temperature at 32 ℃, stirring until the EGCG is dissolved, then adding 1.1kg of maleic anhydride, adding the maleic anhydride for 4 times with equal amount, wherein the time interval between every two adjacent times is 25min, after the addition is finished, continuously stirring to react for 1.5h, and performing rotary evaporation to obtain a solid A;

2) dissolving 8.5kg of palmitoyl pentapeptide-4 in 17kg of THF, and uniformly stirring to obtain a palmitoyl pentapeptide-4 solution;

3) adding the solid A and 3.5kg of carbonyl diimidazole into 22kg of THF, uniformly stirring, controlling the temperature at 8 ℃, then dropwise adding the palmitoyl pentapeptide-4 solution at the dropping speed of 2.5mL/min, continuously stirring for reacting for 2.5h after dropwise adding, and performing rotary evaporation to obtain a solid B;

4) adding the solid B into 45kg of water, controlling the temperature to be 37 ℃, uniformly stirring, filtering to obtain filtrate, performing rotary evaporation on the filtrate to obtain solid C, and performing vacuum drying on the solid C at the temperature of 43 ℃ for 3.5 hours to obtain the modified EGCG.

Preparation example 3

A modified EGCG is prepared by the following steps:

1) adding 5.0kg of EGCG into 25kg of THF, controlling the temperature at 35 ℃, stirring until the EGCG is dissolved, then adding 1.2kg of maleic anhydride, adding the maleic anhydride for 5 times in equal amount, wherein the time interval between every two adjacent times is 20min, after the addition is finished, continuously stirring for reacting for 2h, and performing rotary evaporation to obtain a solid A;

2) dissolving 9kg of palmitoyl pentapeptide-4 in 20kg of THF, and uniformly stirring to obtain a palmitoyl pentapeptide-4 solution;

3) adding the solid A and 4.0kg of carbonyl diimidazole into 25kg of THF, uniformly stirring, controlling the temperature at 10 ℃, then dropwise adding the palmitoyl pentapeptide-4 solution at the dropwise adding speed of 3mL/min, continuously stirring for reacting for 3 hours after dropwise adding, and rotationally evaporating to obtain a solid B;

4) adding the solid B into 50kg of water, controlling the temperature to be 40 ℃, uniformly stirring, filtering to obtain filtrate, performing rotary evaporation on the filtrate to obtain solid C, and performing vacuum drying on the solid C for 3 hours at the temperature of 45 ℃ to obtain the modified EGCG.

Examples

Examples 1 to 3

The deep-sea matsutake moisturizing composition of examples 1-3, comprising a deep-sea matsutake moisturizing essence, a deep-sea matsutake moisturizing muscle base solution, and a deep-sea matsutake multi-vitamin moisturizing cream;

wherein, the raw materials and the dosage of the deep sea pinus hirsuta water-locking essence are shown in table 1, and the preparation steps are as follows:

adding propylene glycol, glycerol, 1, 2-hexanediol, allantoin and ethylhexylglycerin into water, stirring until the propylene glycol, the glycerol, the 1, 2-hexanediol, the allantoin and the ethylhexylglycerin are dissolved, then adding a phaeophyceae extract, a rhodococcus extract, a brown algae extract, a laminaria cuneata extract, a Chondrus crispus extract, a codium tomentosum extract, carbomer and xanthan gum, and stirring until the water is dissolved to obtain a deep-sea codium tomentosum water-locking essence;

wherein, the modified EGCG is obtained from preparation example 1.

TABLE 1 deep-sea Ardisia tomentosa water-locking essence and the amounts (kg) of the raw materials

	Example 1	Example 2	Example 3
				Water (W)	90	95	100
Propylene glycol	1.8	2.0	2.2
				Glycerol	14	12	10
Ascophyllum nodosum extract	0.08	0.10	0.12
				Extract of Rhodococcus	0.15	0.17	0.20
Brown algae extract	0.22	0.20	0.18
				Laminaria ochroleuca extract	0.10	0.06	0.08
Chondrus crispus extract	0.03	0.02	0.01
				Arthodium hirsutum extract	1.2	1.3	1.4
Allantoin	0.20	0.18	0.16
				Carbomer	0.16	0.15	0.14
Xanthan gum	1.4	1.3	1.2
				1, 2-hexanediol	0.8	1.0	1.2
Ethyl hexyl glycerol	1.0	1.2	1.4

The raw materials and the dosage of the raw materials of the deep-sea Arthodium hirsutum moisturizing muscle base solution are shown in Table 2, and the preparation steps are as follows:

adding butanediol, glycerol, betaine, nicotinamide, beta-glucan, sodium hyaluronate, EDTA disodium and 1, 2-hexanediol into water, stirring until the materials are dissolved, then adding hydrolyzed European plum extract, Glycyrrhiza glabra extract, purslane extract, modified EGCG, centella asiatica extract, Hibiscus alpina flower leaf extract, lilium albo-marginatum flower extract and Pinus tomentosa extract, and uniformly stirring to obtain the deep-sea Pinus tomentosa moisturizing foundation solution.

TABLE 2 deep-sea Ardisia tomentosa moisturizing skin base solution and the amount (kg) of each material

	Example 1	Example 2	Example 3
				Water (W)	90	95	100
Butanediol	1.6	1.5	1.4
				Glycerol	2.1	2.2	2.3
Betaine	0.04	0.05	0.06
				Hydrolyzed Prunus humilis extract	0.09	0.08	0.07
Nicotinamide	0.12	0.11	0.10
				Glycyrrhiza glabra extract	0.04	0.06	0.08
Purslane extract	0.09	0.07	0.05
				Beta-glucan	0.14	0.15	0.16
Modified EGCG	2.3	2.5	2.7
				Centella asiatica extract	0.06	0.08	0.10
Extract of leaf of Hibiscus alpinus	0.09	0.08	0.07
				Lilium Candidum extract	0.12	0.11	0.10
Arthodium hirsutum extract	1.4	1.3	1.2
				Hyaluronic acid sodium salt	0.03	0.02	0.01
EDTA disodium salt	1.8	2.0	2.2
				1, 2-hexanediol	1.8	1.6	1.4

The raw materials and the dosage of the raw materials of the deep sea Ardisia tomentosa multi-vitamin water replenishing cream are shown in Table 3, and the preparation steps are as follows:

adding butanediol, sodium hyaluronate, nicotinamide and 1, 2-hexanediol into water, stirring until the butanediol, sodium hyaluronate, nicotinamide and 1, 2-hexanediol are dissolved, then adding carbomer, jojoba seed oil, tocopherol acetate, caprylic glycerate, glyceryl stearate, a phyllocladium extract, caffeine, a porphyridium extract, a brown algae extract, a codium tomentosum extract, a laminaria key extract and a carrageen crispatus extract, and uniformly stirring to obtain the deep sea codium tomentosum multi-vitamin water replenishing cream.

TABLE 3 deep-sea Ardisia tomentosa multi-vitamin moisturizing cream and the amount (kg) of each material

	Example 1	Example 2	Example 3
				Water (W)	40	45	50
Butanediol	12	10	8
				Hyaluronic acid sodium salt	0.8	0.9	1.0
Nicotinamide	1.0	1.2	1.4
				Carbomer	0.22	0.20	0.18
Jojoba seed oil	0.3	0.4	0.5
				Tocopherol acetate	0.10	0.11	0.09
Caprylic acid glycerate	0.05	0.06	0.07
				Glycerol stearate	0.08	0.06	0.04
Caffeine	0.12	0.13	0.14
				Ascophyllum nodosum extract	0.09	0.10	0.11
Porphyridium extract	0.02	0.03	0.04
				Brown algae extract	0.05	0.06	0.07
Arthodium hirsutum extract	1.8	1.7	1.6
				Laminaria ochroleuca extract	0.01	0.02	0.03
Chondrus crispus extract	0.03	0.02	0.04
				1, 2-hexanediol	2	3	4

Example 4

The deep sea Arthosiphon aristatus moisturizing composition of example 4 is different from example 1 in that the modified EGCG is added in an amount of 2.5kg, and the rest steps are the same as example 1.

Example 5

The deep sea Arthosiphon aristatus moisturizing composition of example 5 is different from example 1 in that the modified EGCG is added in an amount of 2.7kg, and the rest steps are the same as example 1.

Example 6

The deep sea Arthosiphon aristatus moisturizing composition of example 6 is different from example 1 in that the modified EGCG is added in an amount of 3.0kg, and the rest steps are the same as those of example 1.

Example 7

The deep sea Arthosiphon aristatus moisturizing composition of example 7 is different from example 1 in that the modified EGCG is from preparation 2, and the rest steps are the same as example 1.

Example 8

The deep sea Arthosiphon aristatus moisturizing composition of example 8 is different from example 1 in that the modified EGCG is from preparation example 3, and the rest steps are the same as example 1.

Comparative example

Comparative example 1

The deep sea codium tomentosum moisturizing composition of comparative example 1 is different from example 1 in that the modified EGCG is added in an amount of 0, and the rest of the steps are the same as those of example 1.

Comparative example 2

A deep-sea codium tomentosum moisturizing composition of comparative example 2 is different from example 1 in that the codium tomentosum extract is added in an amount of 0, and the remaining steps are the same as those of example 1.

Comparative example 3

A deep sea codium tomentosum moisturizing composition of comparative example 3 is different from example 1 in that the modified EGCG is replaced with an equal amount of EGCG, and the remaining steps are the same as example 1.

Comparative example 4

A deep sea pinus hirsuta moisturizing composition of comparative example 4 is different from example 1 in that the modified EGCG was replaced with a mixture of equal amounts of unreacted palmitoyl pentapeptide-4 and EGCG, and the remaining steps are the same as in example 1.

Application example 1

An application method of a deep-sea pinus hirsuta moisturizing composition comprises the following application steps:

1) after skin cleaning, 1mL of deep sea pinus tomentosa water-locking essence is smeared on the face and is gently massaged until absorption;

2) after the step 1), taking 1.5mL of deep-sea Arthodium hirsutum moisturizing skin base solution, smearing the moisturizing skin base solution on the face, and gently massaging the face until the moisturizing skin base solution is absorbed;

3) after the step 2), applying 0.5mL of deep-sea Ardisia tomentosa multi-vitamin water replenishing cream to the face, and gently massaging until absorption.

Application example 2

An application method of a deep-sea pinus hirsuta moisturizing composition comprises the following application steps:

1) after skin cleaning, 1.5mL of deep sea pinus hirsuta water locking essence is smeared on the face and is gently massaged until absorption;

2) after the step 1), taking 1mL of deep-sea Arthodium hirsutum moisturizing skin base solution, smearing the moisturizing skin base solution on the face, and gently massaging the face until the moisturizing skin base solution is absorbed;

3) after the step 2), applying 0.6mL of deep-sea Ardisia tomentosa multi-vitamin water replenishing cream to the face, and gently massaging until absorption.

Application example 3

An application method of a deep-sea pinus hirsuta moisturizing composition comprises the following application steps:

1) after skin cleaning, 1.3mL of deep sea pinus hirsuta water locking essence is smeared on the face and is gently massaged until absorption;

2) after the step 1), taking 1.2mL of deep-sea Arthodium hirsutum moisturizing skin base solution, smearing the moisturizing skin base solution on the face, and gently massaging the face until the moisturizing skin base solution is absorbed;

3) after the step 2), applying 0.7mL of deep-sea Ardisia tomentosa multi-vitamin water replenishing cream to the face, and gently massaging until absorption.

Performance test

Detection method/test method

Corresponding deep sea Ardisia tomentosa water-replenishing compositions were prepared according to the methods of examples 1 to 8 and comparative examples 1 to 4, respectively, and tested by the following methods, respectively, and the test results are shown in Table 4.

Color change: observing the color of the muscle base solution in the deep-sea Arthodium hirsutum moisturizing composition, and observing whether the composition changes color or not after being placed for 1 year at the temperature of 30 ℃ under a sealed condition.

And (3) detecting the moisturizing effect:

1) the test population: 65 dry skin subjects, age 20-50 years, sex random, divided into 13 groups of 5 persons, using a skin moisture tester Corneometer CM825 to measure the facial skin moisture content of the subjects, calculating the average value of each group, and obtaining blank value;

2) the subjects used the deep-sea Arthosiphon aristatus moisturizing compounds prepared in examples 1-7 and comparative examples 1-4 respectively according to groups by the method in application example 1, the last group was a blank control group, and under the same conditions, after 6h, facial skin moisture content of each group of subjects was measured by a skin moisture tester CM825, and the average value of each group was calculated to obtain a test value;

the hydration rate was calculated by the following formula, and the larger the hydration rate, the better the moisturizing effect.

TABLE 4 test results of examples 1 to 8 and comparative examples 1 to 4

	Color change condition	Hydration rate/%)
			Example 1	No obvious color change	115.6
Example 2	No obvious color change	116.0
			Example 3	No obvious color change	115.9
Example 4	No obvious color change	116.0
			Example 5	No obvious color change	116.7
Example 6	No obvious color change	116.8
			Example 7	No obvious color change	115.7
Example 8	No obvious color change	115.5
			Comparative example 1	No obvious color change	96.8
Comparative example 2	No obvious color change	92.3
			Comparative example 3	The color is changed into pink	96.6
Comparison ofExample 4	The color is changed into pink	90.1

From the detection results in table 4, it can be seen that the hydration rate of the deep-sea chaetomium globosum moisturizing composition is maintained to be above 115.6% after the composition is used for 6 hours, which indicates that the deep-sea chaetomium globosum moisturizing composition has a good moisturizing effect.

From the data of example 1 and examples 4 to 6, it can be seen that the moisturizing effect of the obtained deep sea Ardisia tomentosa moisturizing composition increases with increasing amount of the added modified EGCG, but the moisturizing effect increases gradually after the addition amount reaches 2.7 kg.

As can be seen from the data of example 1 and examples 7-8, there is no significant difference in the modified EGCG obtained in preparations 1-3.

As can be seen from the data of example 1 and comparative examples 1-2, the moisturizing effect of the deep sea hirsutella tomentosa moisturizing composition obtained by simultaneously adding the modified EGCG and the hirsutella tomentosa extract is the best when compared with the case of simultaneously adding one of the modified EGCG and the hirsutella tomentosa extract, and the two are proved to have synergistic effect.

As can be seen from the data of example 1 and comparative examples 3-4, the modified EGCG has better stability than EGCG, and the modified EGCG is not easy to deteriorate.

As can be seen from the data of example 1 and comparative example 4, the moisturizing effect of palmitoyl pentapeptide-4 reacted with EGCG was weaker than that of the modified EGCG.

The present embodiment is only for explaining the present application, and it is not limited to the present application, and those skilled in the art can make modifications of the present embodiment without inventive contribution as needed after reading the present specification, but all of them are protected by patent law within the scope of the claims of the present application.

Claims (6)

1. The deep-sea pinus hirsutus moisturizing composition is characterized by comprising a deep-sea pinus hirsutus moisturizing essence, a deep-sea pinus hirsutus moisturizing skin base solution and a deep-sea pinus hirsutus multi-vitamin moisturizing cream;

the deep-sea pinus hirsuta water-locking essence is prepared from the following raw materials in parts by weight: 90-100 parts of water, 1.8-2.2 parts of propylene glycol, 10-14 parts of glycerol, 0.08-0.12 part of Ascophyllum nodosum extract, 0.15-0.20 part of rhodococcus extract, 0.18-0.22 part of brown algae extract, 0.08-0.10 part of Laminaria digitata extract, 0.01-0.03 part of Chondrus crispus extract, 1.2-1.4 parts of Pinus lansidaensis extract, 0.16-0.20 part of allantoin, 0.14-0.16 part of carbomer, 1.2-1.4 parts of xanthan gum, 0.8-1.2 parts of 1, 2-hexanediol and 1.0-1.4 parts of ethylhexylglycerin;

the deep-sea pinus hirsuta moisturizing muscle base solution is prepared from the following raw materials in parts by weight: 90-100 parts of water, 1.4-1.6 parts of butanediol, 2.1-2.3 parts of glycerol, 0.04-0.06 part of betaine, 0.07-0.09 part of hydrolyzed European plum extract, 0.10-0.12 part of nicotinamide, 0.04-0.08 part of glabrous greenbrier rhizome extract, 0.05-0.09 part of purslane extract, 0.14-0.16 part of beta-glucan, 2.3-2.7 parts of modified EGCG, 0.06-0.10 part of snowflake extract, 0.07-0.09 part of alpine edelweiss flower leaf extract, 0.10-0.12 part of white lily flower extract, 1.2-1.4 parts of pinus tomentosa extract, 0.01-0.03 part of sodium hyaluronate, 1.8-2.2 parts of EDTA disodium and 1.4-1.8 parts of 1, 2-hexanediol;

the modified EGCG is obtained by reacting palmitoyl pentapeptide-4, maleic anhydride and EGCG, and the preparation steps of the modified EGCG are as follows:

1) adding 4.5-5.0 parts of EGCG into 20-25 parts of THF, controlling the temperature at 30-35 ℃, stirring until the EGCG is dissolved, then adding 1.0-1.2 parts of maleic anhydride, wherein the time interval between two adjacent times is 20-30min, after the addition is finished, continuing stirring for reaction for 1-2h, and concentrating to obtain a solid A;

2) dissolving 8-9 parts of palmitoyl pentapeptide-4 in 15-20 parts of THF (tetrahydrofuran), and uniformly stirring to obtain a palmitoyl pentapeptide-4 solution;

3) adding the solid A and 3.3-4.0 parts of carbonyl diimidazole into 20-25 parts of THF, uniformly stirring, controlling the temperature at 6-10 ℃, then dropwise adding the palmitoyl pentapeptide-4 solution at the speed of 2-3mL/min, continuously stirring for reacting for 2-3h after dropwise adding, and performing rotary evaporation to obtain a solid B;

4) adding the solid B into 40-50 parts of water, controlling the temperature to be 35-40 ℃, uniformly stirring, filtering to obtain filtrate, concentrating to obtain solid C, and drying to obtain modified EGCG;

the deep sea pinus hirsuta multi-vitamin water replenishing cream is prepared from the following raw materials in parts by weight: 40-50 parts of water, 8-12 parts of butanediol, 0.8-1.0 part of sodium hyaluronate, 1.0-1.4 parts of nicotinamide, 0.18-0.22 part of carbomer, 0.3-0.5 part of jojoba seed oil, 0.09-0.11 part of tocopherol acetate, 0.05-0.07 part of caprylic glycerate, 0.04-0.08 part of glycerol stearate, 0.12-0.14 part of caffeine, 0.09-0.11 part of Ascophyllum nodosum extract, 0.02-0.04 part of Porphyromyces lachnophora extract, 0.05-0.07 part of brown algae extract, 1.6-1.8 parts of Pinus tomentosa extract, 0.01-0.03 part of Laminaria digitata extract, 0.02-0.04 part of Chondrus crispus extract and 2-4 parts of 1, 2-hexanediol.

2. The deep-sea Arthodium hirsutum hydrating composition of claim 1, wherein: and drying by vacuum drying in the step 4), wherein the drying temperature is 40-45 ℃, and the drying time is 3-4 h.

3. The deep-sea Arthodium hirsutum hydrating composition of claim 1, wherein: the preparation method of the deep sea pinus hirsuta moisturizing muscle base solution comprises the following steps: adding butanediol, glycerol, betaine, nicotinamide, beta-glucan, sodium hyaluronate, EDTA disodium and 1, 2-hexanediol into water, stirring until the materials are dissolved, then adding hydrolyzed European plum extract, Glycyrrhiza glabra extract, purslane extract, modified EGCG, centella asiatica extract, Hibiscus alpina flower leaf extract, lilium albo-marginatum flower extract and Pinus tomentosa extract, and uniformly stirring to obtain the deep-sea Pinus tomentosa moisturizing foundation solution.

4. The deep-sea Arthodium hirsutum hydrating composition of claim 1, wherein: the preparation method of the deep sea pinus hirsuta water-locking essence comprises the following steps:

adding propylene glycol, glycerol, 1, 2-hexanediol, allantoin and ethylhexylglycerin into water, stirring until the propylene glycol, the glycerol, the 1, 2-hexanediol, the allantoin and the ethylhexylglycerin are dissolved, then adding the Ascophyllum nodosum extract, the Rhodococcus rhodochrous extract, the brown algae extract, the Laminaria digitata extract, the Chondrus crispus extract, the Pinus hirsuta extract, the carbomer and the xanthan gum, and stirring until the Podocus hirsutus linn water-locking essence is dissolved, so as to obtain the deep-sea Pinus hirsutus linn water-locking essence.

5. The deep-sea Arthodium hirsutum hydrating composition of claim 1, wherein: the deep sea pinus hirsuta multi-vitamin water replenishing cream comprises the following preparation steps:

adding butanediol, sodium hyaluronate, nicotinamide and 1, 2-hexanediol into water, stirring until the butanediol, sodium hyaluronate, nicotinamide and 1, 2-hexanediol are dissolved, then adding carbomer, jojoba seed oil, tocopherol acetate, caprylic glycerate, glyceryl stearate, a phyllocladium extract, caffeine, a porphyridium extract, a brown algae extract, a codium tomentosum extract, a laminaria key extract and a carrageen crispatus extract, and uniformly stirring to obtain the deep sea codium tomentosum multi-vitamin water replenishing cream.

6. The method of using the deep sea Arthodium hirsutum hydrating composition of any of claims 1-5, wherein: which comprises the following steps:

1) after skin cleaning, 1.0-1.5mL of deep sea pinus hirsuta water locking essence is smeared on the face and is gently massaged until absorption;

2) after the step 1), 1.0-1.5mL of deep-sea Aronaea tomentosa moisturizing skin base solution is smeared on the face and is gently massaged until absorption;

3) after the step 2), 0.5-0.7mL of deep sea Ardisia tomentosa multi-vitamin water replenishing cream is smeared on the face and is gently massaged until absorption.