CN112042656A

CN112042656A - Polygonatum cyrtonema rhizome tissue culture seedling-culturing sterilization insect-prevention rooting powder and preparation method thereof

Info

Publication number: CN112042656A
Application number: CN202010796671.9A
Authority: CN
Inventors: 张华通; 张炜; 林云斌; 黄剑雄
Original assignee: Yangshan County Sanlianyang Ecological Agriculture And Forestry Development Co ltd
Current assignee: Yangshan County Sanlianyang Ecological Agriculture And Forestry Development Co ltd
Priority date: 2020-08-10
Filing date: 2020-08-10
Publication date: 2020-12-08

Abstract

The invention discloses a special sterilization insect-proof rooting powder for polygonatum cyrtonema tissue culture industrial rhizome propagation and seedling culture, which consists of the following substances: IBA, 2.4-D, 36BA, KH₂PO₄、KNO₃、FeSO₄·7H₂0、H₃BO₃Nano silver solution, O-dimethyl- (2,2, 2-trichloro-1-hydroxyethyl) phosphonate crystal and 99% of talcum powder. The rooting powder is used in the industrial seedling culture of polygonatum cyrtonema tissue culture of test-tube plantlets, accelerates the rooting of tubers and the germination growth of buds, plays a role in efficient sterilization, ensures the rooting and seedling formation of rhizomes, improves the transplanting survival rate of the test-tube plantlets of the rhizomes, and can be used in other plants propagated by the rhizomes.

Description

Polygonatum cyrtonema rhizome tissue culture seedling-culturing sterilization insect-prevention rooting powder and preparation method thereof

Technical Field

The invention relates to the technical field of planting, in particular to polygonatum cyrtonema rhizome tissue culture seedling-cultivation sterilization insect-prevention rooting powder.

Background

Rhizome plants (such as Polygonatum cyrtonema Hua, potato Solanum tuberosum L., Zingiber officinale of fiscinale, Dioscorea zingiberensis C.H., Wright, Dioscorea opposita optifolia L.) bloom universally without forming seeds, or have few seeds and are difficult to germinate. Therefore, the seeds are difficult to be adopted for the large-scale seedling production. At present, except potatoes, the mass propagation of seedlings cannot be carried out by adopting plant tissue culture technology for most varieties. The rootstock meristem propagation is a relatively-numerous propagation method adopted at present, but the propagation method needs to consume a large amount of original rootstock materials, seedlings cultivated by the method are easy to degenerate, and the subsequent growth speed and the product quality of the seedlings are seriously influenced due to virus infection. In addition, the rootstocks adopted for the rootstock meristem propagation are very easy to cause rottenness and biting by underground pests due to the wounds left during cutting, thereby seriously affecting the seedling rate. Thus, the large-scale production of high-quality seedlings becomes a common problem of such plants.

Polygonatum cyrtonema Hua is sweet and neutral, and has the effects of invigorating qi, nourishing yin, invigorating spleen, moistening lung and tonifying kidney, and can be used for treating diseases caused by spleen deficiency, lung deficiency and kidney deficiency. It is recorded in the miscellaneous records of famous physicians. According to the regulation of pharmacopoeia of the people's republic of China, the medicinal polygonatum rhizome is dried rhizome of polygonatum kingianum, polygonatum rhizome or polygonatum cyrtonema of Liliaceae. Rhizoma polygonati is also a Chinese medicinal material approved by the national Wei Jian Wei and K. The sealwort has high economic value and medicinal value, can be widely applied to raw materials for producing new medicines, the field of chemical industry and the like, and has larger and larger market demand. However, wild polygonatum is slow in growth and breeding and low in survival rate, so that wild resources are scarce, and in recent years, wild polygonatum is excessively artificially harvested, the growth environment is damaged, seeds are disorderly introduced, and the like, so that the wild high-quality resources are increasingly reduced. In recent years, due to the fact that polygonatum is subjected to long-term yield pursuit, manual blind excavation and excessive mining and manual planting are not standard, so that wild polygonatum faces few seed sources, and the quality of polygonatum is reduced year by year. The shortage of excellent germplasm resources becomes the biggest bottleneck for the development of the polygonatum industry, the protection and the utilization of wild polygonatum resources become problems to be solved urgently, the breeding and the standardized cultivation are particularly urgent and important, and the method is an important means for solving the problem of the development of the polygonatum industry.

The method for industrially culturing seedlings by adopting tissue culture rootstocks is a unique and effective method for solving the problem of the bottleneck. However, no one has developed success at present, mainly because the polygonatum cyrtonema plants have special morphological structures, the fleshy root stems grow in soil, explants are difficult to sterilize and become sterile propagation materials, the root stems contain rich polysaccharides and other metabolic secondary substances and are easy to brown, and in addition, polygonatum cyrtonema has dormant biological characteristics, the number of buds of polygonatum cyrtonema needs to be continuously increased like most plant tissue culture in a bud multiplication mode, and the difficulty is very high. Therefore, the method and the reagent for sterilizing the explants to become the sterile propagation material have important research significance.

Disclosure of Invention

The invention aims at providing a sterilization and insect prevention rooting powder for tissue culture seedlings.

The second aspect of the invention aims to provide the application of the rooting powder in the tissue culture seedling raising of polygonatum cyrtonema rhizome.

The third aspect of the present invention is to provide a method for preparing the rooting powder.

The technical scheme adopted by the invention is as follows:

in a first aspect of the invention, the sterilization and insect prevention rooting powder for the tissue culture seedlings comprises the following raw materials: 400-600 ppm IBA, 3-5 ppm 2.4-D, 20-40 ppm 6BA, 900-1100 ppm KH2PO4, 900-1100 ppm KNO3, 50-150 ppm FeSO 4.7H 20, 40-60 ppm H3BO3, 3-5 wt% nano silver ion solution, 4500-5500 ppm O, O-dimethyl- (2,2, 2-trichloro-1-hydroxyethyl) phosphonate and adhesive.

The rooting powder according to the first aspect of the present invention preferably comprises the following raw materials: 500ppm IBA, 4ppm 2.4-D, 30ppm 6BA, 1000ppm KH2PO4, 1000ppm KNO3, 100ppm FeSO 4.7H 20, 50ppm H3BO3, 4 wt% nano silver ion solution, 5000ppm O, O-dimethyl- (2,2, 2-trichloro-1-hydroxyethyl) phosphonate and adhesion agent.

According to the rooting powder of the first aspect of the present invention, more preferably, the concentration of silver ions in the nano silver solution is 100 ppm.

Preferably, the rooting powder according to the first aspect of the present invention is talc as the binder. The purpose of the adhesive is to make the rooting powder adhere better to the plant surface.

According to the rooting powder of the first aspect of the invention, the mesh number of the talcum powder is 900-1100, and preferably 1000.

In a second aspect of the invention, the application of the rooting powder in the first aspect of the invention in polygonatum cyrtonema rhizome tissue culture seedling is provided.

According to the application of the second aspect of the invention, the rooting powder is adhered to the surface of the rootstock in the rooting stage of the plant meristematic propagation seedling culture and/or is adhered to the surface of the rootstock in the transplanting of the tissue culture seedling culture test-tube plantlet.

In a third aspect of the present invention, there is provided a method for preparing a rooting powder according to the first aspect of the present invention, which mainly comprises the following steps:

s1: weighing corresponding raw materials according to the proportion of the first aspect of the invention, dissolving and mixing the raw materials, and then fixing the volume to obtain a mixture A;

s2: drying the mixture A to obtain a mixture B;

s3: and crushing the mixture B into powder, and sieving the powder by using a soil sieve to obtain the rooting powder.

According to the method of the third aspect of the present invention, preferably, the drying temperature in step S2 is 65-75 ℃, the drying time is 2-4 hours, and the drying is continuously repeated 12-16 times.

According to the method of the third aspect of the present invention, more preferably, the drying temperature in step S2 is 71 ℃, the drying time is 3 hours, and the drying is continuously repeated 12-16 times.

More preferably, the soil sieve of step S3 is 400 meshes.

The invention has the beneficial effects that:

1. the invention provides a sterilization insect-proof rooting powder for tissue culture seedlings, which is characterized in that no harmful substance is added from the components in the formula to the production process, the obtained sterilization insect-proof rooting powder is scientific and reasonable in formula, the preparation method is simple and scientific, the preparation is carried out according to the characteristics of the rhizome tissue culture seedling and the rhizome meristematic seedling of polygonatum multiflorum, and the preparation method is also suitable for most other rhizome plants, and the sterilization insect-proof rooting powder provides early-stage nutrients for the rhizome tissue culture seedling of polygonatum multiflorum and the rhizome meristem seedling growth of other rhizome plants, promotes the rapid rooting of the rhizome, prevents the decay of the rhizome and the biting of underground pests, thereby ensuring the seedling formation of the rhizome, improving the test tube transplanting survival rate of the rhizome and accelerating the growth after seedling formation.

2. The rooting powder provided by the invention is used in the transplanting of the polygonatum cyrtonema tissue culture industrialized seedling rhizome test-tube plantlets, high survival rate and seedling rate are obtained, the rooting and bud germination growth of the rhizome are accelerated, the high-efficiency sterilization effect is achieved, the generation of rotting of the rhizome and the biting of underground pests are effectively prevented, the rooting and seedling of the rhizome are ensured, the transplanting survival rate of the rhizome test-tube plantlets is improved, and the rooting powder can be adopted in other plants with meristematic propagation of the rhizome.

3. The formula of the rooting powder provided by the invention is added with nano silver (Ag)⁺Chelate), play a role in high-efficient sterilization, have prevented the rotten production of rhizome effectively; in addition, 90% trichlorfon crystals are added, so that the biting of underground pests is effectively prevented, the seedling formation of the rhizome is ensured, and the transplanting survival rate of the rhizome test-tube plantlet is improved.

4. The rooting powder provided by the invention can be applied to the meristem propagation of old rhizomes of polygonatum cyrtonema of Yangshan, effectively prevents the generation of rhizome decay, prevents the biting of underground pests, efficiently ensures the seedling formation of the rhizomes, improves the seedling formation of the rhizomes, enables the large-scale production of seedlings of polygonatum cyrtonema of Yangshan to be possible, and solves the bottleneck of the industrial development of polygonatum cyrtonema of Yanshan.

5. The root powder can be used in plants propagated by other rhizomes (such as potato, ginger, small yellow ginger, Chinese yam, etc.), so that the problem of low seedling rate of seedlings propagated by other rhizomes is solved, the seedling management procedure is simplified, and the labor cost is saved.

6. The invention also provides a use method of the rooting powder. The polygonatum sibiricum cultivated by tissue culture by adopting the method has the seedling rate of 90-95%, the rooting rate of 95-100% and the test-tube seedling transplanting survival rate of 91-98%; the seedling rate of the rhizome meristem propagation is 95-98%, and the method is adopted to carry out tissue culture and factory rhizome seedling culture production and rhizome meristem propagation, so that a high-quality Yangshan polygonatum cyrtonema rhizome tissue culture seedling 21590 strain and a rhizome meristem propagation seedling 18560 strain are cultured.

Drawings

FIG. 1 shows the prepared and bottled rooting powder.

FIG. 2 tissue culture is transplanted out of the flask rhizome.

FIG. 3 shows the germination of 60 days after the tissue culture of rhizome transplantation rooting powder.

FIG. 4 shows the rooting and germination conditions of the seedlings in 5 months in the next year after the treatment of the old tuber meristem propagation rooting powder.

FIG. 5 shows the growth of tissue culture rhizome after transplanting and planting in 5 months every other year.

Detailed Description

In order to enhance the understanding of the present invention, the present invention will be described in further detail with reference to the following examples, which are provided for the purpose of illustration only and are not intended to limit the scope of the present invention.

The nano silver ion solution is a nano silver (Ag +) D stock solution which is purchased from materials science and technology ltd of Guangzhou city, and the content concentration of the silver ion component is 100 ppm.

Example 1

The embodiment provides a sterilization and insect prevention rooting powder for tissue culture seedlings, and the formula components of the rooting powder are shown in table 1.

TABLE 1 formulation and description of the special bactericidal insect-proof rooting powder

The preparation process of the tissue culture seedling-raising sterilizing insect-preventing rooting powder mainly comprises the following steps:

s1: weighing the corresponding raw materials:

1. weighing talcum powder: wearing a mask and disposable rubber gloves, weighing 990g of talcum powder by an electronic scale, and placing the talcum powder into a 40cm x 60cm stainless steel operating disk.

2.90% O, O-dimethyl- (2,2, 2-trichloro-1-hydroxyethyl) phosphonate was weighed: weighing 5g of 90% O, O-dimethyl- (2,2, 2-trichloro-1-hydroxyethyl) phosphonate, putting the weighed materials into a 1L plastic measuring cup, adding 100mL of purified water to dissolve the materials, uniformly pouring the materials onto the weighed talcum powder, and continuously stirring the materials by using a glass rod.

3. Nano silver (Ag)⁺) D, measuring stock solution: measuring 40mL of nano silver (Ag) by using measuring cylinder⁺) D, putting the stock solution into a 1L plastic measuring cup, diluting the stock solution to 200mL by using purified water, uniformly pouring the diluted stock solution onto the weighed talcum powder, and continuously stirring the mixture by using a glass rod.

4. Weighing other medicines, dissolving and fixing volume: the following were weighed separately with an electronic scale: 0.5g of indolebutyric acid is firstly dissolved by a proper amount of 1N NaOH solution; 0.004g of 2.4-dichlorophenoxyacetic acid is dissolved by using a proper amount of 1N NaOH solution; 0.03g of 6-benzylamino adenine is firstly dissolved by a proper amount of 1N HCl solution; 1g of monopotassium phosphate, 1g of potassium nitrate, 0.1g of ferrous sulfate and 0.05g of boric acid, dissolving the components in 50mL of purified water one by one in a 200mL beaker, pouring the dissolved components into a 200mL volumetric flask for constant volume, slowly and uniformly pouring the weighed talcum powder onto the weighed talcum powder, and continuously stirring the mixture by using a glass rod until the talcum powder is uniformly wetted and leveled.

S2: drying: the prepared talcum powder is put into an electric heating air blast drying box, a door is closed, the temperature is regulated and controlled to be 70 ℃, and drying is carried out at regular time for 3 hours. And (4) a drying process, namely checking the dry and wet degree of the talcum powder at the end of every 3 hours until drying. Generally, the moisture in the talcum powder can be dried only by continuously and repeatedly drying for 8 times.

S3: milling: taking the dried talcum powder out of the drying box, crushing the dried talcum powder into powder, sieving the powder by using a soil sieve of 400 meshes, putting the powder into a 20L plastic basin, continuously crushing the coarse talcum powder particles which cannot pass through the sieve, and rolling while sieving until all the talcum powder is sieved.

S4: packaging and storing: the sieved talcum powder is the tissue culture seedling cultivation sterilization insect prevention rooting powder, and the tissue culture seedling cultivation sterilization insect prevention rooting powder is put into a 500mL white plastic reagent bottle with a label for recording preparation date, product name and use method, sealed and stored in a shady and ventilated and dry place (as shown in figure 1).

Example 2

The embodiment provides a sterilization and insect prevention rooting powder for tissue culture seedlings, which comprises the following raw materials in parts by weight: 400ppm IBA, 3ppm 2.4-D, 20ppm 6BA, 900ppm KH₂PO₄、900ppm KNO₃、50ppm FeSO₄·7H₂0、40ppm H₃BO₃30mL of 100ppm Ag⁺Solution, 4500ppm O, O-dimethyl- (2,2, 2-trichloro-1-hydroxyethyl) phosphonate and an attachment agent.

s1: weighing the corresponding raw materials in proportion:

1. weighing talcum powder: wearing a mask and disposable rubber gloves, weighing 990g of talcum powder by an electronic scale, and placing the talcum powder into a 40cm x 60cm stainless steel operating disk;

2.90% O, O-dimethyl- (2,2, 2-trichloro-1-hydroxyethyl) phosphonate was weighed: weighing 4.5g of 90% O, O-dimethyl- (2,2, 2-trichloro-1-hydroxyethyl) phosphonate, putting into a 1L plastic measuring cup, adding 100mL of purified water for dissolving, uniformly pouring onto the weighed talcum powder, and continuously stirring by using a glass rod;

3. nano silver (Ag)⁺) D, measuring stock solution: 30mL of nano silver (Ag) is measured by a measuring cylinder⁺) D, putting the stock solution into a 1L plastic measuring cup, diluting the stock solution to 200mL by using purified water, uniformly pouring the diluted stock solution onto the weighed talcum powder, and continuously stirring the mixture by using a glass rod.

4. Weighing other medicines, dissolving and fixing volume: the following were weighed separately with an electronic scale: 0.4g of indolebutyric acid is firstly dissolved by a proper amount of 1N NaOH solution; 0.003g of 2.4-dichlorophenoxyacetic acid, and dissolving by using a proper amount of 1N NaOH solution; 0.02g of 6-benzylamino adenine is firstly dissolved by a proper amount of 1N HCl solution; 0.9g of monopotassium phosphate, 0.9g of potassium nitrate, 0.05g of ferrous sulfate and 0.04g of boric acid, dissolving the components in 50mL of purified water one by one in a 200mL beaker, pouring the dissolved components into a 200mL volumetric flask for constant volume, slowly and uniformly pouring the weighed talcum powder onto the weighed talcum powder, continuously stirring the mixture by using a glass rod until the talcum powder is uniformly wetted and leveled.

S2: drying: the prepared talcum powder is put into an electric heating air blast drying box, a door is closed, the temperature is controlled to be 75 ℃, and drying is carried out at regular time for 2 hours. And (4) a drying process, namely checking the dry and wet degree of the talcum powder at the end of every 2 hours until drying. Generally, the moisture in the talcum powder can be dried only by continuously and repeatedly drying for more than 12 times.

S4: packaging and storing: the sieved talcum powder is the tissue culture seedling cultivation sterilization insect prevention rooting powder, and the tissue culture seedling cultivation sterilization insect prevention rooting powder is put into a 500mL white plastic reagent bottle which is stuck with a label for recording the preparation date, the product name and the use method, sealed and stored in a shady, cool and ventilated and dry place.

Example 3

The embodiment provides a sterilization and insect prevention rooting powder for tissue culture seedlings, which comprises the following raw materials in parts by weight: 600ppm IBA, 5ppm 2.4-D, 40ppm 6BA, 1100ppm KH₂PO₄、1100ppm KNO₃、150ppm FeSO₄·7H₂0、60ppm H₃BO₃50mL of 100ppm Ag⁺Solution, 5500ppm O, O-dimethyl- (2,2, 2-trichloro-1-hydroxyethyl) phosphonate and an adhesive.

s1: weighing the corresponding raw materials:

2.90% O, O-dimethyl- (2,2, 2-trichloro-1-hydroxyethyl) phosphonate was weighed: weighing 5.5g of 90% O, O-dimethyl- (2,2, 2-trichloro-1-hydroxyethyl) phosphonate, putting the weighed materials into a 1L plastic measuring cup, adding 100mL of purified water to dissolve the materials, uniformly pouring the materials onto the weighed talcum powder, and continuously stirring the materials by using a glass rod.

3. Nano silver (Ag)⁺) D, measuring stock solution: 50mL of nano silver (Ag) is measured by a measuring cylinder⁺) D, putting the stock solution into a 1L plastic measuring cup, diluting the stock solution to 200mL by using purified water, uniformly pouring the diluted stock solution onto the weighed talcum powder, and continuously stirring the mixture by using a glass rod.

4. Weighing other medicines, dissolving and fixing volume: the following were weighed separately with an electronic scale: 0.6g of indolebutyric acid is firstly dissolved by a proper amount of 1N NaOH solution; 0.005g of 2.4-dichlorophenoxyacetic acid, firstly dissolving with a proper amount of 1N NaOH solution; 0.04g of 6-benzylamino adenine is firstly dissolved by a proper amount of 1NHCl solution; 1.1g of monopotassium phosphate, 1.1g of potassium nitrate, 0.15g of ferrous sulfate and 0.06g of boric acid, dissolving the components in 50mL of purified water one by one in a 200mL beaker, pouring the dissolved components into a 200mL volumetric flask for constant volume, slowly and uniformly pouring the weighed talcum powder onto the weighed talcum powder, continuously stirring the mixture by using a glass rod until the talcum powder is uniformly wetted and leveled.

S2: drying: the prepared talcum powder is put into an electric heating air blast drying box, a door is closed, the temperature is controlled to be 75 ℃, and drying is carried out at regular time for 4 hours. And (4) a drying process, namely checking the dry and wet degree of the talcum powder at the end of every 4 hours until drying. Generally, the moisture in the talcum powder can be dried only by continuously and repeatedly drying for 6 times.

Example 4 Polygonatum cyrtonema test tube plantlet transplanting Effect experiment

The embodiment provides a contrast test of tissue culture seedling-raising sterilization insect-prevention rooting powder on the transplantation effect of polygonatum cyrtonema tissue culture industrialized seedling-raising test-tube seedlings, and the obtained test data is taken as the basis to optimize the formula components, and the method mainly comprises the following steps:

1. and (3) experimental design: 4000 test tube seedlings of rhizomes are selected, each group comprises 1000 seedlings and a seedbed, the test group comprises 1-3 groups and a control group, wherein 1-3 groups adopt the special sterilization insect-proof rooting powder formula for polygonatum cyrtonema tissue culture industrial rhizomes propagation and seedling culture in the embodiment 1-3 groups, the test tube seedling transplantation is carried out in 3 months, the control group does not adopt the formula, and the seedling rate and the growth condition of the test tube seedling are recorded after the transplantation and planting for 60 days.

2. Selecting test facilities: the temporary simple plastic shed is selected under the field environment condition, adopts rain-proof water, and is easy to perform operations such as ventilation, illumination adjustment, temperature adjustment, humidity adjustment, water spraying, fertilization, pest control and the like.

3. Preparing a transplanting bed: the ground of the transplanting shed is higher than the surrounding ground, drainage ditches are arranged around the transplanting shed, the height of the bed edge of the transplanting bed is 15cm, the transplanting shed is built into a width of 1.2m by red bricks for construction, the length of the transplanting shed is specifically determined according to the length, and 40cm footpaths are paved between beds by red bricks for construction. And filling dried and clean river sand on the transplanting bed to serve as a transplanting matrix, wherein the filling height of the transplanting matrix is about 2cm lower than the edge of the bed. And the substrate was disinfected with 0.2% potassium permanganate solution and 500 times 50% sodium diquat solution.

4. Preparing rootstock test-tube plantlets: taking out the bottled test tube seedling with rhizome after seedling hardening in a seedling hardening plastic greenhouse, cleaning, sterilizing, airing and grading.

5. Applying test planting of rooting powder of the test-tube plantlets of the rhizomes: first-level rhizome test-tube seedlings are uniformly selected and planted in different areas according to test design, and the row spacing of the seedlings is 8 multiplied by 8 cm. When planting, after wetting the test-tube plantlets of the rhizomes, dipping and adhering the sterilization and insect prevention rooting powder in the embodiments 1 to 3, ensuring that a small thin layer of rooting powder is uniformly adhered to the surface of each rhizome, and planting the test-tube plantlets of the control group on a transplanting bed without dipping and adhering.

6. Cultivating and domesticating the test-tube plantlets of the rhizomes: and after planting, strictly performing management such as humidity, illumination, moisture, temperature, ventilation, fertilizer content, pest control and the like according to the biological characteristics and ecological habits of polygonatum cyrtonema.

7. Statistical analysis of test data: after the test-tube plantlets are transplanted for 60 days, the seedling rate and the growth condition of the test-tube plantlets are subjected to statistical analysis, and the results are shown in table 2.

TABLE 2 influence of different ratios of rooting powder components on the transplanting seedling rate and growth condition of Polygonatum cyrtonema rhizome test-tube plantlet

As can be seen from the data in table 2, by using the tissue culture seedling-raising, sterilizing and insect-preventing rooting powder in examples 1 to 3, the rate of death and insect bite damage of transplanted rootstock test-tube seedlings due to decay is reduced, the rooting and seedling rate is significantly increased, the rooting and bud germination are accelerated, and the plant growth conditions are good, as shown in fig. 2 to 4.

FIG. 2 shows the rhizome of Polygonatum cyrtonema after tissue culture and transplantation. The figure shows that the shape is formed, but the root is not formed, certain leaf buds grow out, and the rootstock grows under the artificially controlled environmental condition, so that the seedling is weak, and the seedling has no good capability of autonomously absorbing water and nutrients in the matrix.

FIG. 3 shows the germination condition of 60 days after the rhizome of Polygonatum sibiricum tissue culture is transplanted with rooting powder. It can be seen that the root system is formed, the leaf bud is strong, large and tall, and has good capability of independently absorbing water and nutrients in the matrix.

FIG. 4 shows the growth of Polygonatum cyrtonema tissue culture rhizome after rooting powder treatment, transplanting and planting in 5 months every other year. It can be seen that the growth is good at this time. The seedlings are tidy, the seedling rate is high, and the seedlings grow fast and robustly.

Example 5 Polygonatum cyrtonema rhizome meristem propagation seedling raising experiment

The embodiment provides a contrast test of the tissue culture seedling-raising bactericidal insect-prevention rooting powder on the meristematic propagation seedling-raising effect of polygonatum cyrtonema rhizome, and verifies the action effect of the bactericidal insect-prevention rooting powder on the meristematic propagation seedling-raising effect of polygonatum cyrtonema rhizome based on the obtained test data, and the method mainly comprises the following steps:

1. and (3) experimental design: selecting 2000 high-quality polygonatum cyrtonema rhizomes, cutting each rhizome into 3-5 cm in size, containing 1-2 bud eyes, dividing each group into 500 blocks and a seedbed into test groups 1-3 and a control group, wherein 1-3 groups adopt the formula of the polygonatum cyrtonema tissue culture industrialized rhizome propagation seedling special sterilization insect-prevention rooting powder for carrying out rhizome meristem propagation seedling test in 2 months, the control group does not adopt the formula, and recording the seedling rate and growth condition of rhizome meristem propagation after planting for 40 days.

3. Preparing a transplanting bed: the ground of the transplanting shed is higher than the surrounding ground, drainage ditches are arranged around the transplanting shed, the height of the bed edge of the transplanting bed is 15cm, the transplanting shed is built into a width of 1.2m by red bricks for construction, the length of the transplanting shed is specifically determined according to the length, and 40cm footpaths are paved between beds by red bricks for construction. And filling dried and clean river sand on the transplanting bed to serve as a transplanting matrix, wherein the filling height of the transplanting matrix is about 2cm lower than the edge of the bed.

And the substrate was disinfected with 0.2% potassium permanganate solution and 500 times 50% sodium diquat solution.

4. Preparing meristematic and propagating rhizomes of polygonatum cyrtonema: wild healthy and high-quality polygonatum cyrtonema rhizomes without diseases and insect pests are purchased from the market and cut into small rhizomes with the size of 3-5 cm and containing 1-2 bud eyes.

5. Meristematic propagation seedling test of polygonatum cyrtonema: planting according to experimental design in different areas, wherein the row spacing of the plants is 10 multiplied by 10 cm. When planting, after wetting the small rhizomes of polygonatum cyrtonema, dipping and adhering the sterilization and insect prevention rooting powder in the embodiments 1-3, ensuring that a small thin layer of rooting powder is uniformly adhered to the surface of each rhizome, and planting the control group on a transplanting bed without dipping and adhering.

6. Rhizome meristem propagation, seedling culture and cultivation management of polygonatum cyrtonema: and after planting, strictly performing management such as humidity, illumination, moisture, temperature, ventilation, fertilizer content, pest control and the like according to the biological characteristics and ecological habits of polygonatum cyrtonema.

7. Statistical analysis of test data: after the polygonatum cyrtonema small rootstocks are planted for 40 days, the seedling rate and the growth condition are statistically analyzed, and the results are shown in table 3.

TABLE 3 influence of different rooting powder composition ratios on the seedling rate and growth condition of rhizome meristematic propagation of Polygonatum cyrtonema Hua

As can be seen from the data in Table 3, by using the tissue culture seedling-raising, sterilizing and insect-preventing rooting powder in examples 1 to 3, the rate of death and insect bite damage of small roots of planted polygonatum cyrtonema sibiricum due to decay is reduced, the rooting and seedling rate is remarkably improved, the rooting and bud germination are accelerated, and the plant growth condition is good.

FIG. 5 shows the rooting and germination conditions of the aged Polygonatum cyrtonema rhizome after treatment with the root-growing powder for meristematic propagation of the aged Polygonatum cyrtonema rhizome in 3 months in the next year. It can be seen that the growth was good. The germination is uniform, the root system is developed, the rootstocks are thick and strong, and no insect pest occurs.

Example 6 meristem propagation and seedling culture experiment of potato tubers

The embodiment provides a contrast test of the tissue culture seedling-raising sterilizing insect-prevention rooting powder on the meristematic propagation seedling-raising effect of potato tubers, and verifies the effect of the bactericidal insect-prevention rooting powder on the meristematic propagation seedling-raising effect of the potato tubers by using the obtained test data as the basis, and the method mainly comprises the following steps:

1. and (3) experimental design: 2000 high-quality potato tubers are selected, each tuber is cut into 3-5 cm in size and contains 2-3 bud eyes, each group comprises 500 tubers, one seedbed and is divided into a test group 1-3 and a control group, wherein 1-3 groups adopt the special bactericidal insect-proof rooting powder formula for polygonatum cyrtonema tissue culture industrialized rhizome propagation and seedling culture in the embodiment 1-3, the tuber meristem propagation and seedling culture test is carried out in 10 months, the control group is not adopted, and the seedling rate and the growth condition of the rhizome meristem propagation are recorded after 50 days of planting.

4. Preparing potato tubers: high-quality potato tubers without virus, disease and insect pests, which are used for producing seedlings, are purchased from the market and cut into small tubers with the size of 3-5 cm and 2-3 bud eyes.

5. Potato tuber meristem propagation seedling test: planting according to experimental design in different areas, wherein the row spacing of the plants is 10 multiplied by 10 cm. When the small potato tubers are wet, dipping and adhering the special sterilization and insect prevention rooting powder for the polygonatum multiflorum rhizome test-tube seedling transplantation in the embodiments 1-3, ensuring that a small thin layer of rooting powder is uniformly adhered to the surface of each rhizome, and planting the control group on a transplantation bed without dipping and adhering.

6. Potato tuber meristem propagation seedling cultivation management: managing humidity, illumination, moisture, temperature, ventilation, fertilizer, pest control and the like strictly according to the biological characteristics and ecological habits of the potatoes after planting

7. Statistical analysis of test data: after the potato small tubers are planted for 50 days, the seedling rate and the growth condition of the potato small tubers are subjected to statistical analysis, and the results are shown in table 4.

TABLE 4 influence of different root-inducing powder composition ratios on the seedling rate and growth condition of potato tuber meristematic propagation seedling

As can be seen from the data in Table 4, by using the tissue culture seedling-raising, sterilizing and insect-preventing rooting powder in the embodiments 1 to 3, the death rate and insect bite damage rate of the planted potato tubers due to decay are reduced, the rooting and seedling rate is remarkably improved, the rooting and bud germination are accelerated, and the plant growth condition is good.

Example 7 meristem propagation and seedling raising experiment of ginger rhizome

The embodiment provides a contrast test of the tissue culture seedling-raising bactericidal insect-prevention rooting powder on the seedling raising effect of the rhizome meristem propagation of ginger, and verifies the effect of the bactericidal insect-prevention rooting powder on the seedling raising effect of the rhizome meristem propagation of ginger based on the obtained test data, and the method mainly comprises the following steps:

1. and (3) experimental design: selecting 2000 high-quality ginger roots, cutting each root into 3-5 cm in size, containing 1-2 bud eyes, dividing each group into 500 blocks and a seedbed into test groups 1-3 and a control group, wherein 1-3 groups adopt the special sterilization insect-proof rooting powder formula for polygonatum cyrtonema tissue culture industrialized rhizome propagation and seedling culture in 5 months, carrying out a rhizome meristem propagation and seedling culture test, and the control group does not adopt the formula, and recording the seedling rate and growth condition of rhizome meristem propagation after planting for 50 days.

4. Preparing ginger planting rootstocks: healthy and high-quality ginger roots without diseases and insect pests for seedling production are purchased from the market and cut into small roots with the size of 3-5 cm and 1-2 bud eyes.

5. Meristem propagation seedling test of ginger rhizome: planting according to experimental design in different areas, wherein the row spacing of the plants is 10 multiplied by 10 cm. During planting, after small rhizome blocks of ginger are wet, the special sterilization and insect prevention rooting powder for the polygonatum multiflorum rhizome test-tube seedling transplantation in the embodiments 1-3 is dipped and adhered, after a small thin layer of rooting powder is uniformly adhered to the surface of each rhizome, the control group is not dipped and adhered, and the rhizome blocks are planted on a transplantation bed.

6. And (3) meristem propagation, seedling culture and management of ginger rootstocks: after planting, strictly according to the biological characteristics and ecological habits of the ginger, managing humidity, illumination, moisture, temperature, ventilation, fertilizer, pest control and the like

7. Statistical analysis of test data: after the ginger rhizome is planted for 50 days, the seedling rate and the growth condition of the ginger rhizome are subjected to statistical analysis, and the results are shown in Table 5

TABLE 5 influence of different root powder composition ratios on the seedling rate and growth condition of ginger rhizome meristem propagation

As can be seen from the data in Table 5, by using the tissue culture seedling-raising, sterilizing and insect-preventing rooting powder in examples 1 to 3, the rate of death of small pieces of rhizome of ginger due to decay and damage due to insect bite is reduced, the rooting and seedling rate is remarkably improved, the rooting and bud germination are accelerated, and the plant growth condition is good.

The above-mentioned embodiments only express several embodiments of the present invention, and the description thereof is more specific and detailed, but not construed as limiting the scope of the present invention. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the inventive concept, which falls within the scope of the present invention.

Claims

1. The sterilization insect-prevention rooting powder for the tissue culture seedlings comprises the following raw materials: 400-600 ppm IBA, 3-5 ppm 2.4-D, 20-40 ppm 6BA, 900-1100 ppm KH₂PO₄、900～1100ppm KNO₃、50～150ppm FeSO₄·7H₂0、40～60ppm H₃BO₃3-5 wt% of nano silver ion solution, 4500-5500 ppm of O, O-dimethyl- (2,2, 2-trichloro-1-hydroxyethyl) phosphonate and an adhesive.

2. The rooting powder according to claim 1, wherein the bactericidal and insect-resistant rooting powder comprises the following raw materials: 500ppm IBA, 4ppm 2.4-D, 30ppm 6BA, 1000ppm KH₂PO₄、1000ppm KNO₃、100ppm FeSO₄·7H₂0、50ppm H₃BO₃4 wt% nano silver ion solution, 5000ppm O, O-dimethyl- (2,2, 2-trichloro-1-hydroxyethyl) phosphonate and adhesive.

3. The rooting powder according to claim 1 or 2, wherein the concentration of silver ions in the nano-silver solution is 100 ppm.

4. The rooting powder according to claim 1 or 2, wherein the adhesive is talc.

5. The rooting powder according to claim 4, wherein the talc powder has a mesh number of 900 to 1100.

6. Use of the rooting powder of any one of claims 1 to 5 in tissue culture of rhizomes.

7. The use according to claim 6, wherein the rooting powder is adhered to the surface of the rootstock at the rooting stage of the meristematic seedling raising of the plant and/or at the surface of the rootstock at the time of the transplantation of the tissue culture seedling raising test-tube plantlet.

8. The use according to claim 6, wherein the rhizome plant is Polygonatum cyrtonema, Potato or ginger.

9. A process for preparing a rooting powder according to any one of claims 1 to 4, comprising the steps of:

s1: weighing corresponding raw materials according to the proportion of any one of claims 1 to 4, dissolving and mixing, and fixing the volume to obtain a mixture A;

s2: drying the mixture A to obtain a mixture B;

10. The method according to claim 8, wherein the drying temperature in step S2 is 65-75 ℃, the drying time is 2-4 h, and the drying is continuously repeated for 12-16 times.