CN112042495A - Sweet potato virus-free seedling culture method - Google Patents
Sweet potato virus-free seedling culture method Download PDFInfo
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- CN112042495A CN112042495A CN202011074004.6A CN202011074004A CN112042495A CN 112042495 A CN112042495 A CN 112042495A CN 202011074004 A CN202011074004 A CN 202011074004A CN 112042495 A CN112042495 A CN 112042495A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/25—Root crops, e.g. potatoes, yams, beet or wasabi
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
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Abstract
The invention discloses a virus-free sweet potato seedling raising method, which comprises the following steps: (1) basic culture: selecting sweet potato tubers free of insect pests and disease spots, sterilizing, soaking in normal-temperature water for 24h, and covering with wet cloth for accelerating germination for 20-25 days; (2) detoxification treatment of sweet potato seedlings: after the sweet potato buds grow out, digging down the sweet potato buds and the surrounding sweet potatoes, culturing for 25-35 days on a detoxification culture medium, and simultaneously performing heat treatment at the temperature of 34-40 ℃; (3) sweet potato seedling close planting: taking the pretreated sweet potato seedling tips, carrying out close planting, cutting off the seedlings at the top end part after the close planting is carried out for 25-30 days, and then carrying out close planting; (4) transplanting: and cutting the sweet potato seedlings after close planting, and transferring to the field for cultivation. The method strictly controls the temperature, accelerates the growth of the sweet potato seedlings, shortens the seedling raising time, adopts a heat treatment detoxification mode, has short time consumption and good detoxification effect, effectively prevents diseases, is economical and practical, and greatly improves the survival rate of the seedling.
Description
Technical Field
The invention belongs to the technical field of agricultural planting, and particularly relates to a sweet potato virus-free seedling raising method.
Background
Sweet potatoes are annual herbaceous plants, the underground part is a round, oval or spindle-shaped root tuber, and the shape, the skin color and the flesh color of the root tuber are different according to different varieties or soil. The stem is horizontal or ascended, occasionally winding, multi-branch, cylindrical or rectangular, green or purple, and is sparse and soft or hairless, and adventitious roots are easy to grow on the stem node. The sweet potato is rich in protein, starch, pectin, cellulose, amino acid, vitamin and various mineral substances, and has the reputation of longevity food. The sugar content reaches 15 to 20 percent. Has anticancer, heart protecting, emphysema and diabetes preventing, and weight reducing effects. The Ming Dynasty Li Shizhen Ben Cao gang mu records that the sweet potato has the functions of tonifying deficiency, strengthening spleen and appetizing, and strengthening kidney yin, and says that people in the sea eat for a long life, and the sweet potato is regarded as a good medicine in traditional Chinese medicine. Therefore, sweet potato planting is favored by people, the traditional method is mostly adopted for the current sweet potato planting, however, viruses are easily infected in the seedling raising process, the quality of the sweet potatoes is reduced, and the yield is reduced. Therefore, improvement of the existing seedling raising method is urgently needed to ensure the quality of sweet potatoes, increase the yield and improve the income of farmers.
Disclosure of Invention
In order to solve the problems, the invention aims to provide a virus-free sweet potato seedling raising method.
A sweet potato virus-free seedling raising method comprises the following steps:
(1) basic culture: selecting sweet potato tubers free of insect pests and disease spots, sterilizing, soaking in normal-temperature water for 24h, and covering with wet cloth for accelerating germination for 20-25 days;
(2) detoxification treatment of sweet potato seedlings: after the sweet potato buds grow out, digging down the sweet potato buds and the surrounding sweet potatoes, culturing for 25-35 days on a detoxification culture medium, and simultaneously performing heat treatment at the temperature of 34-40 ℃;
(3) sweet potato seedling close planting: taking the pretreated sweet potato seedling tips, carrying out close planting, cutting off the seedlings at the top end part after the close planting is carried out for 25-30 days, and then carrying out close planting;
(4) transplanting: and cutting the sweet potato seedlings after close planting, and transferring to the field for cultivation.
In order to shorten the sprouting time, the sprouting temperature is 22-25 ℃ during the basic culture.
In order to ensure complete detoxification, the detoxification culture medium comprises the following formula components of 0.5-1mg/L, NAA 0.2.2-0.3 mg/L of MS +6-BA, 8-12g/L of maltose, 85-95mg/L of inositol and 2-5g/L of brown rice flour
Further, the scorched rice flour is coked at high temperature under vacuum condition, the coking rate is 85%, and the scorched rice flour is obtained after crushing.
Compared with the prior art, the sweet potato virus-free seedling culture method has the beneficial effects that: the method strictly controls the temperature, accelerates the growth of sweet potato seedlings, shortens the seedling raising time, adopts a heat treatment detoxification mode, has short time consumption and good detoxification effect, effectively prevents diseases, thereby improving the yield, increasing the economic benefit of farmers, being economical and practical, and greatly improving the survival rate of the seedling raising.
Detailed Description
The principles and features of this invention are described below in conjunction with examples, which are set forth to illustrate, but are not to be construed to limit the scope of the invention.
Example 1
A sweet potato virus-free seedling raising method comprises the following steps:
(1) basic culture: selecting sweet potato tubers free of insect pests and disease spots, sterilizing, soaking in normal-temperature water for 24h, and covering with wet cloth for accelerating germination for 20 days;
(2) detoxification treatment of sweet potato seedlings: after the sweet potato buds grow out, digging down the sweet potato buds and the surrounding sweet potatoes, culturing for 25 days on a detoxification culture medium, and simultaneously carrying out heat treatment at the temperature of 34 ℃;
(3) sweet potato seedling close planting: taking the pretreated sweet potato seedling tips, carrying out close planting, cutting off the seedlings at the top end part after 25 days of close planting, and then carrying out close planting;
(4) transplanting: and cutting the sweet potato seedlings after close planting, and transferring to the field for cultivation.
In order to shorten the germination time, the temperature for accelerating germination is 22 ℃ during the basic culture.
In order to ensure complete detoxification, the detoxification culture medium comprises the following formula components of 0.5mg/L, NAA 0.2.2 mg/L of MS +6-BA, 8g/L of maltose, 85mg/L of inositol and 2g/L of brown rice flour
Further, the scorched rice flour is coked at high temperature under vacuum condition, the coking rate is 85%, and the scorched rice flour is obtained after crushing.
Example 2
A sweet potato virus-free seedling raising method comprises the following steps:
(1) basic culture: selecting sweet potato tubers free of insect pests and disease spots, sterilizing, soaking in normal-temperature water for 24h, and covering with wet cloth for accelerating germination for 22 days;
(2) detoxification treatment of sweet potato seedlings: digging the sweet potato buds and the surrounding sweet potatoes after the sweet potato buds grow out, culturing the sweet potato buds and the surrounding sweet potatoes on a detoxification culture medium for 30 days, and simultaneously carrying out heat treatment at 37 ℃;
(3) sweet potato seedling close planting: taking the pretreated sweet potato seedling tips, carrying out close planting, cutting off the seedlings at the top end part after 28 days of close planting, and then carrying out close planting;
(4) transplanting: and cutting the sweet potato seedlings after close planting, and transferring to the field for cultivation.
In order to shorten the germination time, the temperature for accelerating germination is 23 ℃ during the basic culture.
In order to ensure complete detoxification, the detoxification culture medium comprises the following formula components of 0.8mg/L, NAA 0.25.25 mg/L of MS +6-BA, 10g/L of maltose, 90mg/L of inositol and 3g/L of brown rice flour
Further, the scorched rice flour is coked at high temperature under vacuum condition, the coking rate is 85%, and the scorched rice flour is obtained after crushing.
Example 3
A sweet potato virus-free seedling raising method comprises the following steps:
(1) basic culture: selecting sweet potato tubers free of insect pests and disease spots, sterilizing, soaking in normal-temperature water for 24h, and covering with wet cloth for accelerating germination for 25 days;
(2) detoxification treatment of sweet potato seedlings: after the sweet potato buds grow out, digging down the sweet potato buds and the surrounding sweet potatoes, culturing for 35 days on a detoxification culture medium, and simultaneously carrying out heat treatment at the temperature of 40 ℃;
(3) sweet potato seedling close planting: taking the pretreated sweet potato seedling tips, carrying out close planting, cutting off the seedlings at the top end part after 30 days of close planting, and then carrying out close planting;
(4) transplanting: and cutting the sweet potato seedlings after close planting, and transferring to the field for cultivation.
In order to shorten the germination time, the temperature for accelerating germination is 25 ℃ during the basic culture.
In order to ensure complete detoxification, the detoxification culture medium comprises the following formula components of MS +6-BA 1mg/L, NAA 0.3.3 mg/L, maltose 12g/L, inositol 95mg/L and brown rice flour 5g/L
Further, the scorched rice flour is coked at high temperature under vacuum condition, the coking rate is 85%, and the scorched rice flour is obtained after crushing.
The method strictly controls the temperature, accelerates the growth of sweet potato seedlings, shortens the seedling raising time, adopts a heat treatment detoxification mode, has short time consumption and good detoxification effect, effectively prevents diseases, thereby improving the yield, increasing the economic benefit of farmers, being economical and practical, and greatly improving the survival rate of the seedling raising.
The scope of the present invention is not limited to the technical solutions disclosed in the embodiments, and any modifications, equivalent substitutions, improvements, etc. made to the above embodiments according to the technical spirit of the present invention fall within the scope of the present invention.
Claims (4)
1. A sweet potato virus-free seedling method is characterized in that: the method comprises the following steps:
(1) basic culture: selecting sweet potato tubers free of insect pests and disease spots, sterilizing, soaking in normal-temperature water for 24h, and covering with wet cloth for accelerating germination for 20-25 days;
(2) detoxification treatment of sweet potato seedlings: after the sweet potato buds grow out, digging down the sweet potato buds and the surrounding sweet potatoes, culturing for 25-35 days on a detoxification culture medium, and simultaneously performing heat treatment at the temperature of 34-40 ℃;
(3) sweet potato seedling close planting: taking the pretreated sweet potato seedling tips, carrying out close planting, cutting off the seedlings at the top end part after the close planting is carried out for 25-30 days, and then carrying out close planting;
(4) transplanting: and cutting the sweet potato seedlings after close planting, and transferring to the field for cultivation.
2. The virus-free seedling raising method for sweet potatoes as claimed in claim 1, which is characterized in that: and during the basic culture, the germination accelerating temperature is 22-25 ℃.
3. The virus-free seedling raising method for sweet potatoes as claimed in claim 1 or 2, which is characterized in that: the formula of the detoxification culture medium comprises 0.5-1mg/L, NAA 0.2.2-0.3 mg/L of MS +6-BA, 8-12g/L of maltose, 85-95mg/L of inositol and 2-5g/L of brown rice flour.
4. The virus-free seedling raising method for sweet potatoes as claimed in claim 3, which is characterized in that: the brown rice flour is obtained by coking rice grains at high temperature under vacuum condition with a coking rate of 85%, and pulverizing.
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104396490A (en) * | 2014-10-31 | 2015-03-11 | 颍上县永祥旱粮研究所 | Sweet potato seedling-raising method |
| CN105210869A (en) * | 2015-09-30 | 2016-01-06 | 耿跃 | A kind of method of bighead atractylodes rhizome detoxifying fast breeding |
| CN107409669A (en) * | 2017-04-22 | 2017-12-01 | 蚌埠市乔峰农业蔬菜专业合作社 | A kind of sweet potato is heat-treated poison-removing method |
| CN108834797A (en) * | 2018-05-24 | 2018-11-20 | 吴莉 | A kind of method for culturing seedlings of nuisanceless ipomoea batatas seedling |
| US20190059247A1 (en) * | 2017-08-30 | 2019-02-28 | Mianyang Teachers' College | Cultivation Method for the Rapid Propagation of Davidia Involucrata Winter Buds |
| CN111418467A (en) * | 2020-05-11 | 2020-07-17 | 冉智峰 | Sweet potato seedling raising method |
-
2020
- 2020-10-10 CN CN202011074004.6A patent/CN112042495A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104396490A (en) * | 2014-10-31 | 2015-03-11 | 颍上县永祥旱粮研究所 | Sweet potato seedling-raising method |
| CN105210869A (en) * | 2015-09-30 | 2016-01-06 | 耿跃 | A kind of method of bighead atractylodes rhizome detoxifying fast breeding |
| CN107409669A (en) * | 2017-04-22 | 2017-12-01 | 蚌埠市乔峰农业蔬菜专业合作社 | A kind of sweet potato is heat-treated poison-removing method |
| US20190059247A1 (en) * | 2017-08-30 | 2019-02-28 | Mianyang Teachers' College | Cultivation Method for the Rapid Propagation of Davidia Involucrata Winter Buds |
| CN108834797A (en) * | 2018-05-24 | 2018-11-20 | 吴莉 | A kind of method for culturing seedlings of nuisanceless ipomoea batatas seedling |
| CN111418467A (en) * | 2020-05-11 | 2020-07-17 | 冉智峰 | Sweet potato seedling raising method |
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