CN112033918A

CN112033918A - Biochemical analyzer

Info

Publication number: CN112033918A
Application number: CN202010938594.6A
Authority: CN
Inventors: 赖远强; 朱海科; 吴岸峰; 景振辉
Original assignee: Guangzhou Wondfo Biotech Co Ltd
Current assignee: Guangzhou Wondfo Biotech Co Ltd
Priority date: 2020-09-09
Filing date: 2020-09-09
Publication date: 2020-12-04
Also published as: WO2022052364A1

Abstract

The invention discloses a biochemical analyzer. The biochemical analyzer is used for analyzing and testing a sample to be tested on the test paper. The biochemical analyzer comprises a light path component, a first heating component, a second heating component and a shell component. The test paper component comprises test paper; the light path component comprises a light source component, and the light source component is used for emitting light to irradiate the test paper; the first heating assembly comprises a tray body and a first heating element, the tray body is used for placing the test paper, and the first heating element is used for heating the lower surface of the test paper; the second heating component comprises a second heating element, and the second heating element is used for heating the upper surface of the test paper; the optical path assembly is located within the housing assembly. The biochemical analyzer can reduce the influence of the environmental temperature on the test result.

Description

Biochemical analyzer

Technical Field

The invention relates to the technical field of analysis and test, in particular to a biochemical analyzer.

Background

A biochemical analyzer, also called biochemical analyzer, is a biochemical detection device for detecting and feeding back multiple samples or multiple items. The detection principle of the biochemical analyzer is to detect a certain characteristic chemical component in a sample based on the photoelectric colorimetric principle. The biochemical analyzer has the advantages of high detection speed, high accuracy, low consumption of dosage and the like, and is widely applied to places such as hospitals, epidemic prevention stations, quarantine stations and the like.

The current biochemical analyzer mainly comprises a sample adding system, a software system and the like. However, the test results of the biochemical instruments on the existing market are generally greatly influenced by the ambient temperature.

Disclosure of Invention

The invention mainly aims to provide a biochemical analyzer, so that the test result is less influenced by the ambient temperature.

The embodiment of the invention provides a biochemical analyzer. The biochemical analyzer is used for analyzing and testing a sample to be tested on the test paper. The biochemical analyzer includes:

the light path component comprises a light source component, and the light source component is used for emitting light to irradiate the test paper;

the first heating assembly comprises a tray body and a first heating element, the tray body is used for placing the test paper, and the first heating element is used for heating the lower surface of the test paper;

the second heating assembly comprises a second heating element, and the second heating element is used for heating the upper surface of the test paper; and

a housing assembly, the optical path assembly located within the housing assembly.

The biochemical analyzer in this embodiment heats the upper and lower surfaces of the test paper, and can heat the test paper to a required working temperature, thereby reducing the influence of the ambient temperature on the test result.

In some embodiments, the first heating assembly has a first temperature sensing element for detecting the temperature of the lower surface of the test strip and a first over-temperature protection switch electrically connected to the first temperature sensing element, and the second heating assembly has a second temperature sensing element for detecting the temperature of the upper surface of the test strip and a second over-temperature protection switch electrically connected to the second temperature sensing element. The temperature sensing element detects that the temperatures of the upper surface and the lower surface of the test paper exceed the required working temperature, and the temperature protection switch can stop the heating element from working, so that the working temperature of the test paper is basically in a constant temperature state.

In some embodiments, the first temperature sensing element is mounted on the tray body by a thermally conductive silicone; the second heating assembly further comprises a heating element fixing support, and the second temperature sensing element is installed on the heating element fixing support through heat-conducting silica gel. The heat conducting silica gel can accelerate the heat transfer.

In some embodiments, the tray body further has a storage structure for containing a target for light intensity calibration of the light source assembly. The tray subassembly has stored and has designed the calibration thing, when placing the test paper subassembly and will await measuring the sample advance kind, can carry out the light intensity calibration to every group LED lamp of light source subassembly to shorten test time and improve test reliability.

In some embodiments, the storage structure includes a first fixing groove for receiving the scale, and a first fixing cover detachably fixedly coupled to a top of the first fixing groove. The detachably connected mode of fixed slot and fixed lid for the storage of calibration object is more reliable, and makes things convenient for getting of calibration object to put.

In some embodiments, the test strip assembly further comprises a first support member and a second support member arranged up and down, the test strip is located between the first support member and the second support member, the first support member has a first through hole, and the second support member has a second through hole. The first supporting element and the second supporting element are respectively positioned above and below the test paper body and clamp the test paper body. The user can centre gripping whole test paper subassembly through two support element, simple structure, cost are lower and convenient to use.

In some embodiments, the test strip has a reagent membrane layer and a permeation membrane layer between the reagent membrane layer and the first support element, the reagent membrane layer being coated with a chemical reagent. The chemical agent is capable of causing incident light to be partially absorbed after passing through the agent film layer. The permeable membrane layer is used for permeating the sample to be detected in a liquid form to the next layer from top to bottom.

In some embodiments, the biochemical analyzer further includes a scanning component and a control unit, the test strip has encoded information thereon, the scanning component is configured to scan the encoded information to identify a type of the test strip and transmit the type of the test strip to the control unit, and the control unit is configured to control an on-off state of the light source component. The one-dimensional code, the two-dimensional code, and the three-dimensional code are all readable by a machine device, so that the type of the test paper can be identified.

In some embodiments, the light source assembly includes a first LED lamp assembly having a wavelength of 405nm, a second LED lamp assembly having a wavelength of 550nm, and a third LED lamp assembly having a wavelength of 610 nm. The biochemical analyzer in this embodiment includes three different wavelengths, enriching the testable objects of the biochemical analyzer.

In some embodiments, the housing assembly is an enclosed structure. The shell assembly with the closed structure provides mounting positions for all assemblies inside the shell assembly and isolates external light so as to avoid the influence of the external light on the light path assembly.

Drawings

FIG. 1 is a schematic structural diagram of a housing assembly in an embodiment of the invention;

FIG. 2 is a schematic assembly view of the entry and exit assembly according to an embodiment of the present invention;

FIG. 3 is an exploded view of the hatch assembly in an embodiment of the present invention;

FIG. 4 is a schematic structural diagram of a scanning assembly according to an embodiment of the present invention;

FIG. 5 is a schematic diagram of an assembly structure of an optical circuit assembly according to an embodiment of the present invention;

FIG. 6 is an exploded view of an optical circuit assembly according to an embodiment of the present invention;

FIG. 7 is a cross-sectional view of an optical circuit assembly in an embodiment of the present invention;

FIG. 8 is a schematic diagram of an assembly structure of the tray assembly according to an embodiment of the present invention;

FIG. 9 is an exploded view of the tray assembly in an embodiment of the present invention;

FIG. 10 is a schematic diagram of an assembly structure of a pressure block assembly according to an embodiment of the present invention;

FIG. 11 is a cross-sectional view of a compact assembly in an embodiment of the invention;

FIG. 12 is an exploded view of the housing assembly in an embodiment of the present invention;

FIG. 13 is an exploded view of the test strip assembly in an embodiment of the present invention;

FIG. 14 is a schematic diagram of an assembly structure of the test strip assembly according to an embodiment of the present invention.

Reference numerals:

10-a biochemical analyzer;

100-a housing assembly;

200-an in-out cabin assembly; 210-a third scaffold; 220-a step motor; 221-a first screw part, 222-a second screw part, 223-a fixed flange seat; 230-tray changeover elements; 240-guide rail, 241-guide rail mounting seat; 250-optical couplers; 260-a bearing; 270-optical coupling baffle plate;

300-a scanning component, 310-a scanner, 320-a scanner holder;

400-an optical path component; 410-a first LED lamp assembly, 411-a first LED lamp, 412-a first sleeve, 413-a first convex mirror, 414-a first PCBA board, 415-a first convex mirror fixed cover; 420-a second LED lamp assembly; 430-a third LED lamp assembly; 440-a third heating element; 461-first fixed seat; 470-a photodiode;

500-a tray assembly; 510-a tray body, 511-a tray upper cover, 512-a tray lower cover and 513-a test paper component fixing groove; 520-a first heating element, 521-a first temperature sensing element; 522-first over-temperature protection switch; 530-calibration object, 531-calibration object storage tank and 532-calibration object fixing cover; 541-a heat insulation pad;

600-a briquetting assembly; 610-an actuation mechanism; 611-a first bracket, 6111-a first bracket upper fixing cover, 6112-a first bracket lower fixing cover; 612-a second bracket; 613-an electromagnet; 614-guide shaft, 6141-guide shaft thread head; 615-linear bearing, 6151-bearing fixing cover, 6152-bearing buffer ring; 616-a compression spring; 617-an electromagnet connecting element; 618-briquetting body; 620-a heating mechanism; 621-a second heating element; 622-second over-temperature protection switch; 623-a second temperature sensing element;

700-test paper assembly; 710-first support element, 711-first through hole; 720-second support element, 721-second through hole; 730-a permeate membrane layer; 740-a reagent film layer; 750-identification code layer.

Detailed Description

Reference will now be made in detail to embodiments of the present invention, examples of which are illustrated in the accompanying drawings, wherein like or similar reference numerals refer to the same or similar elements or elements having the same or similar function throughout. The embodiments described below with reference to the accompanying drawings are illustrative only for the purpose of explaining the present invention, and are not to be construed as limiting the present invention. The technical solutions between the embodiments of the present invention may be combined with each other, but should be based on the realization of those skilled in the art.

In the description of the present invention, it is to be understood that the terms "upper", "lower", "front", "rear", and "left", "right", etc. indicate orientations or positional relationships based on those shown in the drawings, and are only for convenience of description and simplicity of description, but do not indicate or imply that the referred device or element must have a specific orientation, be constructed in a specific orientation, and be operated, and thus, should not be construed as limiting the present invention.

In the description of the present invention, it is to be noted that, unless otherwise explicitly specified or limited, the term "connected" is to be interpreted broadly, and may be, for example, a fixed connection and a movable connection, a detachable connection and a non-detachable connection, or an integral connection; may be mechanically or electrically connected or may be in communication with each other. And "fixedly connected" includes detachably connected, non-detachably connected, integrally connected, and the like.

In the present invention, references to terms like "first" or "second" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. In the present invention, references to the like "vertical" and "horizontal" do not refer to absolute vertical and absolute horizontal only, but also include generally vertical and generally horizontal as is acceptable in the art.

An embodiment of the present invention provides a biochemical analyzer 10. Referring to fig. 1 to 14, a biochemical analyzer 10 is used for analyzing and testing a sample to be tested on a test strip. The biochemical analyzer 10 includes an optical path component 400, a first heating component, a second heating component, and a housing component 100.

The light path assembly 400 includes a light source assembly for emitting light to illuminate the test paper.

The first heating assembly comprises a tray body 510 and a first heating element 520, wherein the tray body 510 is used for placing the test paper, and the first heating element 520 is used for heating the lower surface of the test paper. Specifically, as shown in fig. 8-9, the first heating assembly is a tray assembly 500.

The second heating assembly includes a second heating element 621, and the second heating element 621 is used for heating the upper surface of the test paper. Specifically, as shown in fig. 10 to 12, the second heating assembly is a briquette assembly 600.

The light path assembly 400 is located within the housing assembly 100.

Specifically, referring to fig. 1, the housing assembly 100 is a box structure. The housing assembly 100 includes a base plate, an upper sealing plate, a right sealing plate, and a left sealing plate (the left sealing plate also serves as a left motor bracket, i.e., the third bracket 210 in fig. 2 to 3), which are connected to the housing through screws to form a stroke-closed box. Fig. 1 also shows that the first wire shaft portion 221 extends out of the housing assembly 100.

In some embodiments, referring to fig. 8 to 12, the first heating element has a first temperature sensing element 521 and a first over-temperature protection switch 522 electrically connected to the first temperature sensing element 521, the second heating element has a second temperature sensing element 623 and a second over-temperature protection switch 622 electrically connected to the second temperature sensing element 623, the first temperature sensing element 521 is used for detecting the temperature of the lower surface of the test strip, and the second temperature sensing element 623 is used for detecting the temperature of the upper surface of the test strip. The temperature sensing element detects that the temperatures of the upper surface and the lower surface of the test paper exceed the required working temperature, and the temperature protection switch can stop the heating element from working, so that the working temperature of the test paper is basically in a constant temperature state.

In some embodiments, the first temperature sensing element 521 is mounted on the tray body 510 by a thermally conductive silicone (not shown); the second heating assembly further includes a heating element fixing bracket on which the second temperature sensing element 623 is mounted through a heat conductive silicone (not shown). The heat-conducting silica gel has good heat conductivity and good electrical insulation, and also has wider use temperature. The heat conducting silica gel can accelerate the heat transfer.

Specifically, as shown in fig. 8-9, the first heating assembly is a tray assembly 500; as shown in fig. 10 to 12, the second heating unit is a briquette unit 600, and the heating element fixing bracket is a first bracket 611.

In some embodiments, referring to fig. 8 to 9, the tray body 500 further has a storage structure for accommodating the scale 530, and the scale 530 is used for light intensity calibration of the light source assembly. The tray subassembly has stored and has designed the calibration thing, when placing the test paper subassembly and will await measuring the sample advance kind, can carry out the light intensity calibration to every group LED lamp of light source subassembly to shorten test time and improve test reliability.

In some embodiments, referring to fig. 8 to 9, the storage structure includes a first fixing groove for receiving the scale 530, and a first fixing cover detachably fixedly coupled to a top of the first fixing groove. Specifically, the first fixing groove is a scale storage groove 531, and the first fixing cover is a scale fixing cover 532. The matching mode of the fixed groove and the fixed cover ensures that the storage of the calibration object is more reliable; the detachable connection mode is convenient for the fetching and the placing of the calibration object.

In some embodiments, referring to fig. 8-9, the first heating element in the biochemical analyzer 10 is a tray assembly 500. The tray assembly 500 includes a tray body 510, a tray upper cover 511, a tray lower cover 512, a scale fixing cover 532, a first temperature sensing element 521, a first over-temperature protection switch 522, a first heating element 520, and a heat insulation pad 541. The tray body 510 is provided therein with a test paper assembly fixing groove 513 and a calibration material storage groove 531. The target storage groove 531 stores the target 530 therein.

Assembly process of the tray assembly 500: the scale fixing cover 532 fixes the scale 530 into the scale storage groove 531 of the tray body 510 by means of the screw coupling member; the first temperature sensing element 521, the first over-temperature protection switch 522 and the first heating element 520 are coated with heat-conducting silica gel and are respectively assembled to corresponding positions of the tray body 510; the tray upper cover 511 and the tray lower cover 512 are fixed on the tray body 510 through threaded connection elements, and the two integrally wrap the outer surface of the tray body 510, and only the test paper assembly fixing groove 513 is exposed, so as to reduce heat loss; the tray assembly 500 is fixed to the hatch-in and hatch-out assembly 200 by means of screw coupling elements (specifically, fixed to the third mounting surface of the tray transit element 230 by means of screw coupling elements), and a heat insulation pad 541 is provided between the tray body 510 and the tray transit element 230, so that effective thermal insulation is achieved between the tray body 510 and the tray transit element 230, thereby reducing heat loss.

The tray assembly 500 functions to: fixing and heating the lower surface of the strip assembly 700 (heating the tray body 510 to a design temperature by the cooperation of the first heating element 520 and the first temperature sensing element 521, and transferring heat to the lower surface of the strip assembly 700 through the strip assembly fixing grooves 513 of the tray body 510); the calibration object 530 is stored for calibration of the optical path of the biochemical analyzer 10.

In some embodiments, referring to fig. 10-12, the second heating element in the biochemical analyzer 10 is a briquetting element 600. As shown in fig. 10 to 12, the compact assembly 600 may be generally divided into a heating mechanism 620, an actuating mechanism 610, a compact body 618, and a second holder 612. The heating mechanism 620 generally includes a first bracket 611, a second temperature sensing element 623, a second heating element 621, a second over-temperature protection switch 622, a first bracket upper stationary cover 6111, a first bracket lower stationary cover 6112, and an electromagnet connecting element 617. The second temperature sensing element 623 may employ a temperature probe. The first bracket 611 is provided with a pressing block body 618. The actuating mechanism 610 includes a compression spring 616, a bearing cage 6152, a linear bearing 615, a bearing retainer cap 6151, an electromagnet 613, and a guide shaft 614. The guide shaft 614 includes a guide shaft threaded head 6141.

The assembly process of the compact assembly 600 can be briefly described as follows: (1) the heating mechanism 620: the second temperature sensing element 623, the second heating element 621 and the second over-temperature protection switch 622 are assembled to corresponding positions of the first bracket 611 by coating heat-conducting silica gel; the upper fixing cover 6111 of the first bracket, the lower fixing cover 6112 of the first bracket and the electromagnet connecting element 617 are made of plastic materials, and the three are fixed to the first bracket 611 through threaded connecting elements, so that the heating element is wrapped and the heat insulation effect is achieved, and heat dissipation is prevented.

(2) The actuating mechanism 610: the electromagnet 613 is assembled with a slot of the electromagnet connecting element 617; the bearing fixing cover 6151 fixes the bearing buffer ring 6152 and the linear bearing 615 to the second bracket 612 through a threaded connection element; the guide shaft 614 passes through the linear bearing 615 and the compression spring 616, and the guide shaft 614 is locked to the screw hole of the first bracket 611 through the guide shaft threaded head 6141, so that the guide shaft 614 is communicated with the actuating mechanism 610, and the guide and elastic pressing functions are achieved.

The briquetting assembly 600 functions as follows: the upper surface of the test paper is heated when the whole biochemical analyzer works, and the briquetting assembly 600 is automatically lifted when the cabin entering and exiting assembly 200 works so as to prevent the tray assembly 500 from being damaged.

In some embodiments, referring to fig. 13 to 14, the test strip assembly 700 further includes a first supporting member 710 and a second supporting member 720 disposed above and below the test strip, the test strip is disposed between the first supporting member 710 and the second supporting member 720, the first supporting member 710 has a first through hole 711, and the second supporting member 720 has a second through hole 721.

The first supporting element and the second supporting element are respectively positioned above and below the test paper body and clamp the test paper body. The user can centre gripping whole test paper subassembly through two support element, simple structure, cost are lower and convenient to use.

In some embodiments, referring to fig. 13-14, the test strip has a reagent layer 740 and a permeable layer 730, the permeable layer 730 is located between the reagent layer 740 and the first support member 720, and the reagent layer 740 is coated with a chemical reagent. The chemical reagent can partially absorb incident light after the incident light passes through the reagent film layer, and diffuse reflection is generated after unabsorbed light reaches the permeation film layer, and the light is reflected out of the test paper assembly. The permeable membrane layer is used for permeating the sample to be detected in a liquid form to the next layer from top to bottom. The permeable membrane layer can be made of hydrophilic materials, so that the permeable diffusion of a sample to be detected is facilitated.

Specifically, referring to fig. 13-14, the test strip assembly 700 includes a second support member 720, a reagent membrane 740, a permeation membrane 730, a first support member 710, and an identification code layer 750. The test paper assembly 700 is a laminated structure, and includes a first supporting element 710, a permeable membrane layer 730, a reagent membrane layer 740, and a second supporting element 720 from top to bottom. The first supporting element 710 is provided with a first through hole 711, and the first through hole 711 is used for sample injection; the second support member 720 has a second through hole 721, and the second through hole 721 is for passing light emitted from the optical path component and returning.

The surface of the second supporting member 720 may be divided into a first area and a second area, wherein the first area coincides with the first supporting member 710, and the second supporting member 720 further has a second area beyond the first supporting member 710 and not coinciding. The identification code layer 750 is attached to the second region. Specifically, the identification code layer 750 may be a barcode label layer.

The reagent membrane layer 740 is coated with a different chemical reagent that causes incident light to be partially absorbed by the reagent membrane layer 740, and the unabsorbed incident light is reflected off the strip assembly after reaching the lower surface of the permeable membrane layer 730. An identification code layer 750 is designed on the test paper for distinguishing different test papers.

The operation of the test strip assembly 700 can be briefly described as follows: after the sample to be measured is dropped from the first through hole 711 of the first supporting element 710, the sample to be measured diffuses and permeates through the permeation membrane layer 730 to the whole membrane layer, and a diffuse reflection layer is formed on the lower surface of the permeation membrane layer 730; the light emitted by the LED lamp assembly in the light path assembly is partially absorbed after passing through the reagent film layer 740, and the rest of the light is reflected in the diffuse reflection layer; the reflected light is absorbed by the photodiode after passing through the reagent film layer 740. The degree of light absorption is measured as absorbance, and the correlation formula is: the absorbance is — lg (reflected light/incident light), and the biochemical analyzer 10 calculates a parameter value of the corresponding parameter of the sample to be measured from the absorbance.

In some embodiments, referring to fig. 4, the biochemical analyzer 10 further includes a scanning assembly 300 and a control unit (not shown), the test strip has encoded information thereon, the scanning assembly 300 is used for scanning the encoded information to identify the type of the test strip and transmitting the type of the test strip to the control unit, and the control unit is used for controlling the on/off state of the light source assembly.

Specifically, as shown in fig. 4, the scanning assembly 300 includes a scanner 310 and a scanner holder 320. The scanner 310 is fixed to the scanner holder 320 by a screw connection element, and the scanner holder 320 is fixed to the upper sealing plate of the housing assembly 100 by a screw connection element. When the biochemical analyzer is in operation, the scanner 310 scans the coded information (e.g., one-dimensional code) on the test strip, so as to identify the parameter type of the test strip and transmit the parameter type to a control unit (e.g., CPU) of the biochemical analyzer, so as to control the on/off state of the LED lamp.

The code includes at least one of a one-dimensional code, a two-dimensional code, and a three-dimensional code. The three-dimensional code is an array which encodes a binary string obtained by text compilation and image information into a set of interpretable binary strings by a specific algorithm in combination with the overall color content of the picture. The one-dimensional code, the two-dimensional code, and the three-dimensional code are all readable by a machine device, so that the type of the test paper can be identified. Specifically, the code may be a one-dimensional code or a two-dimensional code.

In some embodiments, referring to fig. 1, the housing assembly 100 is an enclosed structure. The shell assembly with the closed structure provides mounting positions for all assemblies inside the shell assembly and isolates external light so as to avoid the influence of the external light on the light path assembly. It should be noted that the enclosure assembly of the closed structure does not mean that all the working components of the biochemical analyzer are completely located inside the enclosure assembly, and some components are located outside the enclosure assembly, so that the requirement of isolating the external light can still be met.

In some embodiments, referring to fig. 5-7, the biochemical analyzer 10 further includes an optical path component 400. The optical path component 400 is used as a core component of the biochemical analyzer 10, and is used for realizing the test of the test paper based on the photoelectric colorimetric principle. As shown in fig. 5 to 7, the light path assembly 400 includes a first LED lamp assembly 410 (wavelength of 405nm), a second LED lamp assembly 420 (wavelength of 550nm), a third LED lamp assembly 430 (wavelength of 610nm), a third heating element 440, a photodiode 470 and a first fixing base 461 (made of aluminum substrate).

Different sample components to be tested have specific wavelengths respectively, and the absorbance at the specific wavelength is usually in positive correlation with the concentration of the component, so that the concentration of various components needs to be tested and analyzed by adopting different wavelengths. The biochemical analyzer in the embodiment of the invention comprises three different wavelengths, so that the testable objects of the biochemical analyzer are enriched.

Each of the components of the optical circuit assembly 400 is fixed to the first fixing base 461 by a screw connection member. The first LED lamp assembly 410, the second LED lamp assembly 420, and the third LED lamp assembly 430 are identical in structure. Taking the first LED lamp assembly 410 as an example, the first LED lamp assemblies 410 each include a first LED lamp 411, a first sleeve 412, a first convex mirror 413, a first convex mirror fixing cover 415, and a first PCBA board 414. The first sleeve 412 is made of aluminum base material, and the first sleeve 412 has a light hole therein.

The light source assembly includes a first LED lamp assembly having a wavelength of 405nm, a second LED lamp assembly having a wavelength of 550nm, and a third LED lamp assembly having a wavelength of 610 nm. Different sample components to be tested have specific wavelengths respectively, and the absorbance at the specific wavelength is usually in positive correlation with the concentration of the component, so that the concentration of various components needs to be tested and analyzed by adopting different wavelengths.

The operation of the optical path assembly 400 can be briefly described as follows: after the biochemical analyzer 10 identifies the type of the test paper through the scanning component 300, the corresponding LED lamp is controlled to be powered on. As shown in fig. 7, taking the first LED lamp assembly 410 as an example, light generated by the LED lamp is transmitted through the light-transmitting hole of the first sleeve 412 to irradiate the surface of the first convex mirror 413, and is refracted along the first convex mirror 413 to generate a parallel light beam. In fig. 7, the direction of the arrow indicates the direction of the optical path. As shown in fig. 7, the parallel light beam irradiates the test paper reagent film layer 740 on the tray body 510, and the reagent film layer 740 absorbs a part of the light; the remaining light is reflected to the photodiode 470.

By comparing the difference between the light intensity of the light beam emitted from the LED lamp as the light source and the light intensity of the light beam absorbed by the photodiode 470, the biochemical analyzer 10 converts the corresponding parameter value of the sample to be tested based on the photoelectric colorimetric principle, thereby completing the test of the sample to be tested.

Research shows that the light intensity of the light beam generated by the LED lamp under the same current can be different under different environmental temperatures. To solve this problem, the optical path component 400 of the biochemical analyzer 10 in the embodiment of the present invention further designs a third heating element 440. The third heating element 440 is made of an aluminum substrate, and a heating circuit and a third temperature sensing element (not shown) are disposed in the third heating element 440. The third temperature sensing element may employ a temperature probe. The back surface of the third heating element 440 is coated with a thermally conductive silicone, and the third heating element 440 is fixed to the upper surface of the first fixing base 461 through a screw connection element. When the third heating element 440 is powered on to operate, the third heating element 440 heats the first fixing seat 461 and transfers heat to the sleeve and the LED lamp, so that the operating temperature of the optical path assembly 400 is stabilized at about a desired temperature (e.g., 37 ℃), thereby reducing the influence of the ambient temperature on the light intensity of the LED lamp.

In some embodiments, referring to fig. 2-3, the biochemical analyzer 10 further includes an access module 200. The entry and exit module 200 is used to connect the tray assembly 500, and the automatic entry and exit of the tray assembly 500 and the automatic entry are realized. As shown in fig. 2 to 3, the entry and exit compartment assembly 200 includes a stepping motor 220, a tray changeover element 230, an optical coupler 250, a guide rail 240, and a bearing 260. The number of optocouplers 250 is two. The stepping motor 220, the two optical couplers 250, and the guide rail 240 are fixed to the left cover plate (i.e., the third bracket 210) of the housing assembly 100 by a screw coupling member. The stepping motor 220 is provided with a fixed flange seat 223, the stepping motor 220 is further connected with a first screw rod part 221 and a second screw rod part 222, and the first screw rod part 221 and the second screw rod part 222 are coaxial. The guide rail 240 is provided with a guide rail mounting seat 241, three mutually perpendicular surfaces of the tray switching element 230 are mounting surfaces, and the tray switching element 230 is further provided with an optical coupling baffle 270. Three mounting surfaces are designed on the tray adapter element 230, a first mounting surface is fixed on the guide rail mounting base 241 through a threaded connection element, a second mounting surface is fixedly connected with the fixing flange base 223 through a threaded connection element, and the second mounting surface is fixedly connected with the tray assembly 500.

In some embodiments, the biochemical analyzer 10 includes the housing assembly 100, the entry and exit chamber assembly 200, the scanning assembly 300, the optical path assembly 400, the tray assembly 500, the compact assembly 600, and the test strip assembly 700 described in the previous embodiments. The structure and composition of the above components are not described in detail.

It is understood that the test strip assembly 700 may be provided as a separate component from the biochemical analyzer 10, i.e., in some embodiments, the biochemical analyzer 10 may not include the test strip assembly 700.

The following text will describe the operation of the biochemical analyzer 10 having the above-described components:

(1) the tray assembly 500 is automatically extended: the electromagnet 613 in the briquetting assembly 600 is electrified to work to drive the heating mechanism 620 to ascend, so that the briquetting body 618 is separated from the surface of the tray assembly 500; the stepping motor 220 of the in-out cabin assembly 200 works to drive the tray assembly 500 to extend to a designed position (the optical coupler 250 is used for detecting the movement position of the tray assembly 500);

(2) a calibration process: the third heating element 440 is electrically operated so that the overall temperature of the optical path assembly 400 is maintained at about 37 ℃; the first LED lamp assembly 410, the second LED lamp assembly 420 and the third LED lamp assembly 430 are respectively turned on, and the emitted light passes through the test paper assembly 700 and then is reflected to the photodiode 470, so that the light intensity of each group of LED lamps can be calibrated.

(3) The placement and sample injection process of the test strip assembly 700: the test strip assembly 700 is placed in the test strip assembly fixing groove 513 of the tray assembly 500, and the liquid of the sample to be tested is dropped into the first through hole 711 of the test strip assembly 700 by using a syringe or a pipette, and the liquid of the sample to be tested is automatically diffused to the whole permeation membrane layer 730.

(4) The tray assembly 500 is automatically retracted: the stepping motor 220 of the entrance and exit module 200 works to drive the tray module 500 to retract to a designed position (the optical coupler 250 is used for detecting the movement position of the tray module 500); the electromagnet 613 in the pressing block assembly 600 is powered off, and the heating mechanism 620 descends under the action of the elastic force of the compression spring 616 and the self gravity, so that the pressing block body 618 presses the upper surface of the test paper assembly 700.

(5) The heating process: the first heating element 520 of the tray assembly 500 is electrically operated to heat the tray body 510; the second temperature sensing element 623 detects the temperature of the tray body 510, and the control unit controls the power of the first heating element 520 to keep the temperature of the tray body 510 at about 37 ℃; heat is transferred to the lower surface of the strip assembly 700 through the strip assembly fixing grooves 513 of the tray body 510.

When the temperature of the tray body 510 is too high, the first over-temperature protection switch 522 automatically de-energizes the first heating element 520 to avoid damaging the tray assembly 500; the second heating element 621 of the briquette assembly 600 is electrically operated to heat the first support 611; under the cooperative action of the second temperature sensing element 623 and the second over-temperature protection switch 622, the temperature of the first support 611 is maintained at about 37 ℃, thereby heating the upper surface of the test strip assembly 700.

By heating the upper and lower surfaces on both sides, the test strip assembly 700 can be heated and kept at about 37 ℃, thereby reducing the influence of the ambient temperature on the test result.

(6) The test procedure: after the tray assembly 500 is retracted to the design position (the optical coupler 250 is used to detect the movement position of the tray assembly 500), the scanner 310 is powered on. The scanner 310 scans and transmits encoded information (e.g., one-dimensional code) on the test strip assembly 700 to the control unit. The control unit identifies the type of the test paper assembly 700 to be tested through the coded information and controls the corresponding LED lamp to be electrified and operated.

The light emitted by the LED lamp assembly in the light path assembly is partially absorbed after passing through the reagent film layer 740, and the rest of the light is reflected in the diffuse reflection layer; the reflected light is absorbed by the photodiode after passing through the reagent film layer 740. The degree of light absorption is measured as absorbance, and the correlation formula is: the absorbance is — lg (reflected light/incident light), and the biochemical analyzer 10 calculates a parameter value of the corresponding parameter of the sample to be measured from the absorbance.

The biochemical analyzer 10 of the present embodiment has at least the following technical effects:

(1) the upper surface and the lower surface of the test paper assembly 700 are heated, so that the test paper is rapidly heated and is kept at a constant temperature of about 37 ℃;

(2) the rear end of the tray assembly 500 is provided with a calibration object 530, and during the process of placing the test paper assembly 700 and sample injection, the light intensity of each group of LED lamps of the light source assembly 400 can be calibrated;

(3) the third heating element 440 in the optical path assembly 400 can heat the LED lamp assembly and the like, so that the LED lamp assembly is thermostated to about 37 ℃, thereby reducing the influence of the change of the ambient temperature on the test result;

(4) the biochemical analyzer 10 has a simple structure, reliable test results, short test time and low cost. The entire biochemical analyzer 10 does not require a complicated liquid path structure nor a complicated heat insulating structure. Moreover, the testing process is highly automated-the user only needs to put test paper and complete sample injection, and the subsequent testing can be automatically completed by the biochemical analyzer 10.

The light path component of the biochemical analyzer in the embodiment of the invention is provided with three LED lamps with different wavelengths and a photodiode, and adopts a photoelectric colorimetric principle based on the Lambert beer absorption theory to measure different parameters such as GLU (glucose), TP (total protein), AST (aspartate aminotransferase), GGT (glutamine aminotransferase), CHE (cholinesterase) and CREA (creatinine) in body fluid (containing trace whole blood, plasma, serum or urine).

While embodiments of the invention have been shown and described, it will be understood by those of ordinary skill in the art that: various changes, modifications, substitutions and alterations can be made to the embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the claims and their equivalents.

Claims

1. A biochemical analyzer for performing an analytical test on a sample to be tested on a test strip, the biochemical analyzer comprising:

2. The biochemical analyzer according to claim 1, wherein the first heating unit has a first temperature sensing element for detecting the temperature of the lower surface of the test strip and a first over-temperature protection switch electrically connected to the first temperature sensing element, and the second heating unit has a second temperature sensing element for detecting the temperature of the upper surface of the test strip and a second over-temperature protection switch electrically connected to the second temperature sensing element.

3. The biochemical analyzer of claim 2, wherein the first temperature sensing element is mounted on the tray body by thermally conductive silicone; the second heating assembly further comprises a heating element fixing support, and the second temperature sensing element is installed on the heating element fixing support through heat-conducting silica gel.

4. The biochemical analyzer of claim 1, wherein the tray body further has a storage structure for holding a target for light intensity calibration of the light source assembly.

5. The biochemical analyzer according to claim 4, wherein the storage structure comprises a first fixing groove for receiving the calibration objects and a first fixing cover detachably fixedly attached to a top of the first fixing groove.

6. The biochemical analyzer according to claim 1, wherein the test strip assembly further includes a first support member and a second support member arranged up and down, the test strip being positioned between the first support member and the second support member, the first support member having a first through hole, the second support member having a second through hole.

7. The biochemical analyzer of claim 6, wherein the test strip has a reagent membrane layer and a permeation membrane layer, the permeation membrane layer being located between the reagent membrane layer and the first support element, the reagent membrane layer being coated with a chemical reagent.

8. The biochemical analyzer according to claim 1, further comprising a scanning component and a control unit, wherein the test strip has encoded information thereon, the scanning component is configured to scan the encoded information to identify a type of the test strip and transmit the type of the test strip to the control unit, and the control unit is configured to control an on/off state of the light source component.

9. The biochemical analyzer according to claim 1, wherein the light source assembly includes a first LED light assembly having a wavelength of 405nm, a second LED light assembly having a wavelength of 550nm, and a third LED light assembly having a wavelength of 610 nm.

10. The biochemical analyzer of any one of claims 1 to 9, wherein the housing assembly is an enclosed structure.