CN211697825U - Chromatography analyzer for detecting colloidal gold and fluorescence immunity in same machine - Google Patents
Chromatography analyzer for detecting colloidal gold and fluorescence immunity in same machine Download PDFInfo
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- CN211697825U CN211697825U CN202020329867.2U CN202020329867U CN211697825U CN 211697825 U CN211697825 U CN 211697825U CN 202020329867 U CN202020329867 U CN 202020329867U CN 211697825 U CN211697825 U CN 211697825U
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Abstract
The utility model relates to the field of optical instruments, and discloses a chromatography analyzer for detecting colloidal gold and fluorescence immunity on the same machine, which comprises a detection assembly, wherein the detection assembly comprises a fixing piece, one side of the fixing piece is connected with a LED circuit board, the other side of the fixing piece is connected with a PD circuit board, the top of the fixing piece is connected with a mainboard, the fixing piece comprises a first fixing piece, a second fixing piece and a third fixing piece which are sequentially arranged in parallel and are respectively vertical to the LED circuit board, a colloidal gold detection light path is arranged between the first fixing piece and the second fixing piece, and a fluorescence immunity detection light path is arranged between the second fixing piece and the third fixing piece; the LED circuit board is provided with two LED lamps, the PD circuit board is provided with two detection PD elements, and the LED circuit board and the PD circuit board are respectively connected with the mainboard. The utility model provides a pair of detect colloidal gold and fluorescence immunity's chromatography appearance with machine, the functional stronger and integrated level is higher, small in size, the operation and carry of being convenient for.
Description
Technical Field
The utility model relates to an optical instrument field especially relates to a chromatography analyzer of detecting colloidal gold and fluorescence immunity with machine.
Background
With the attention of people to food safety and medical health industry, the demand of POCT (point-of-care testing) products is explosively increased later, but domestic POCT products are started later, the product structure is complex, the function is single, the price is expensive, the detection precision is low, the product integration level is not high, most of equipment or products are designed for hospitals or detection institutions, the problems of volume or carrying convenience are not considered too much, and certain professional technical requirements and the like are provided for operators.
At present, on one hand, the demand of people is increasing, and on the other hand, the current situation of the existing POCT products is, so that a product which is simple in structure, low in price, small in size, convenient to carry, convenient to operate, high in detection precision and product integration level and suitable for various detection environments is urgently needed.
SUMMERY OF THE UTILITY MODEL
Technical problem to be solved
The utility model aims at providing a detect colloidal gold and fluorescence immunity's chromatography appearance with machine for solve or partially solve current optical detection class instrument structure complicacy, the volume is great, be not portable and the problem of function singleness.
(II) technical scheme
In order to solve the technical problem, the utility model provides a chromatography analyzer for simultaneously detecting colloidal gold and fluorescence immunity, which comprises a detection component, wherein the detection component comprises a fixing part, a LED circuit board, a PD circuit board and a mainboard;
the fixing parts comprise a first fixing part, a second fixing part and a third fixing part which are sequentially arranged in parallel and are respectively vertical to the LED circuit board, a colloidal gold detection light path is arranged between the first fixing part and the second fixing part, and a fluorescence immunoassay detection light path is arranged between the second fixing part and the third fixing part;
the LED circuit board is provided with two LED lamps correspondingly connected with the colloidal gold detection light path and the fluorescence immunoassay detection light path, the PD circuit board is provided with two detection PD elements correspondingly connected with the colloidal gold detection light path and the fluorescence immunoassay detection light path, and the LED circuit board and the PD circuit board are respectively connected with the mainboard.
On the basis of the scheme, the LED circuit board is also provided with two feedback PD elements, the LED lamp connected with the colloidal gold detection light path is simultaneously connected with one feedback PD element, and the LED lamp connected with the fluorescence immunoassay detection light path is also simultaneously connected with one feedback PD element.
On the basis of the scheme, in the colloidal gold detection light path, the LED lamp and the detection PD element are respectively connected with the top end of a vertically arranged focusing mirror, and the bottom of the focusing mirror is correspondingly provided with a straight mirror; in the fluorescence immunoassay light path, an LED lamp and a detection PD element are respectively connected with the top end of another vertically arranged focusing mirror, an optical filter is arranged between the LED lamp and the focusing mirror, and the bottom of the focusing mirror is also correspondingly provided with a straight mirror; the two linear mirrors are respectively positioned at the bottom of the detection assembly.
On the basis of the scheme, in the colloidal gold detection light path, the LED lamp and the PD detection element are respectively connected with a focusing mirror through glass fibers; in the fluorescence immunoassay light path, one side of the optical filter is arranged towards the LED lamp, and the other side of the optical filter and the detection PD element are respectively connected with the other focusing mirror through glass fibers.
On the basis of the scheme, all parts of the colloidal gold detection light path are embedded between the first fixing piece and the second fixing piece; and each part of the fluorescence immunoassay light path is embedded between the second fixing piece and the third fixing piece.
On the basis of the scheme, two sides of the bottom of the detection assembly are respectively connected with the guide rails, the guide rails are arranged on the bottom plate, the detection card is placed between the guide rails on the two sides, the first end of each guide rail is connected with the front vertical plate, the front vertical plate is vertically connected to the bottom plate, and the front vertical plate is provided with the card inserting port.
On the basis of the scheme, the second end of the guide rail is connected with a rear vertical plate, the rear vertical plate is vertically connected to the bottom plate, the detection assembly is driven by a motor to horizontally move along the guide rail, and the motor is fixed on the rear vertical plate.
On the basis of the scheme, guide columns are arranged on two sides of the detection assembly respectively along the guide rails, penetrate through the detection assembly and are provided with bearing sleeves between the detection assembly.
On the basis of the scheme, a matched positioning piece is arranged between the detection assembly and the guide rail.
On the basis of the scheme, an elastic element is arranged on the inner wall of the guide rail; and a limiting part is arranged on the guide rail close to the second end.
(III) advantageous effects
The utility model provides a chromatography analyzer for detecting colloidal gold and fluorescence immunity in the same machine, which integrates the colloidal gold detection light path and the fluorescence immunity detection light path into a detection component, can realize the detection of the analyzer on the colloidal gold and the fluorescence immunity, and has stronger functionality, higher integration level, small volume and convenient operation and carrying; and two LED lamps are welded on one circuit board, and two PD detection elements are welded on the other circuit board, so that the production cost and the assembly and debugging difficulty can be greatly reduced, and the production efficiency is improved.
Drawings
FIG. 1 is a three-dimensional structure diagram of a chromatography analyzer for simultaneously detecting colloidal gold and fluorescence immunity according to an embodiment of the present invention;
fig. 2 is a schematic view illustrating disassembly of the detecting assembly according to an embodiment of the present invention;
FIG. 3 is a schematic diagram of a colloidal gold detection optical path according to an embodiment of the present invention;
FIG. 4 is a schematic diagram of a fluorescence immunoassay optical path according to an embodiment of the present invention;
FIG. 5 is a schematic diagram of fluorescence immunoassay according to an embodiment of the present invention;
fig. 6 is a schematic diagram of colloidal gold detection in the embodiment of the present invention.
Description of reference numerals:
wherein, 1, a bottom plate; 2. a guide rail; 3. a front vertical plate; 4. glass beads; 5. a detection component; 6. a flange plate; 7. detecting the card; 8. a guide post; 9. a hexagon socket head cap screw; 10. an optical coupling baffle plate; 11. a rear vertical plate; 12. a stepping motor; 13. a copper pillar; 14. an expansion board; 15. a first fixing member; 16. a PD circuit board; 17. pressing a plate; 18. a line mirror; 19. a second fixing member; 20. a focusing mirror; 21. a bearing housing; 22. glass fiber; 23. a main board; 24. an optical filter; 25. a silica gel pad; 26. a third fixing member; 27. an LED circuit board; 28. a groove-shaped optical coupler; 29. an LED lamp; 30. detecting the PD element; 31. a focusing lens; 32. and feeding back the PD element.
Detailed Description
The following detailed description of the embodiments of the present invention is provided with reference to the accompanying drawings and examples. The following examples are intended to illustrate the invention, but are not intended to limit the scope of the invention.
In the description of the present invention, it is to be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present invention can be understood in specific cases to those skilled in the art.
Referring to fig. 1 and 2, the embodiment of the utility model provides a with chromatography analyzer that detects colloidal gold and fluorescence immunity, this chromatography analyzer includes determine module 5, determine module 5 includes mounting, LED circuit board 27, PD circuit board 16 and mainboard 23, one side of mounting is connected LED circuit board 27, the opposite side of mounting is connected PD circuit board 16, the top of mounting is connected mainboard 23.
The fixing piece comprises a first fixing piece 15, a second fixing piece 19 and a third fixing piece 26 which are sequentially arranged in parallel and are respectively perpendicular to the LED circuit board 27, a colloidal gold detection light path is arranged between the first fixing piece 15 and the second fixing piece 19, and a fluorescence immunoassay detection light path is arranged between the second fixing piece 19 and the third fixing piece 26.
The LED circuit board 27 is provided with two LED lamps 29 correspondingly connected with the colloidal gold detection light path and the fluorescence immunoassay light path, the PD circuit board 16 is provided with two detection PD elements 30 correspondingly connected with the colloidal gold detection light path and the fluorescence immunoassay light path, and the LED circuit board 27 and the PD circuit board 16 are respectively connected with the mainboard 23.
The detection unit 5 is a component that mainly performs detection. When colloidal gold detection is performed, the operation of the LED lamp 29 corresponding to the colloidal gold detection light path on the LED circuit board 27 is controlled through the main board 23, the light emitted by the LED lamp 29 is detected by colloidal gold through the colloidal gold detection light path, and the detection PD element 30 corresponding to the colloidal gold detection light path receives the light signal of the colloidal gold detection light path and converts the light signal into an electric signal, so that a colloidal gold detection result is obtained.
When the fluorescence immunoassay is performed, the mainboard 23 controls the LED lamp 29 corresponding to the fluorescence immunoassay light path on the LED circuit board 27 to operate, the light emitted by the LED lamp 29 performs the fluorescence immunoassay through the fluorescence immunoassay light path, and the detection PD element 30 corresponding to the fluorescence immunoassay light path receives the light signal of the fluorescence immunoassay light path and converts the light signal into an electrical signal, thereby obtaining the fluorescence immunoassay result.
According to the chromatography analyzer for detecting colloidal gold and fluorescence immunity in the same machine, the colloidal gold detection light path and the fluorescence immunity detection light path are integrated to form the detection component 5, so that the detection of the analyzer on the colloidal gold and the fluorescence immunity can be realized, the functionality is strong, the integration level is high, the size is small, and the operation and the carrying are convenient; and two LED lamps 29 are welded on one circuit board, and two PD detection elements 30 are welded on the other circuit board, so that the production cost and the assembly and debugging difficulty can be greatly reduced, and the production efficiency is improved.
On the basis of the above embodiment, further, the LED circuit board 27 is further provided with two feedback PD elements 32, the LED lamp 29 connected to the colloidal gold detection optical path is simultaneously connected to one feedback PD element 32, and the LED lamp 29 connected to the fluorescence immunoassay detection optical path is also simultaneously connected to one feedback PD element 32.
Namely, the colloidal gold detection optical path and the fluorescence immunoassay detection optical path are respectively and correspondingly provided with a feedback PD element 32. The feedback PD element 32 is provided on the LED circuit board 27. Specifically, for the colloidal gold detection optical path, the LED lamp 29 is connected to two branches, one branch is connected to the feedback PD element 32, and the other branch is connected to the detection PD element 30 through another optical device; a part of the light emitted from the LED lamp 29 is directly received by the feedback PD element 32, and another part is received by the detection PD element 30 after passing through the detection card 7 to be detected via the optical path.
For the fluorescence immunodetection optical path, the LED lamp 29 is also connected to two branches, one branch is connected to the feedback PD element 32, and the other branch is connected to the detection PD element 30 through other optical devices; a part of the light emitted from the LED lamp 29 is directly received by the feedback PD element 32, and another part of the light passes through the detection card 7 to be detected through the optical path and is received by the detection PD element 30.
The test result is obtained by calculating the values of the feedback PD element 32 and the detection PD element by comparing the standard detection substance and the analyte, respectively. The double PDs are adopted for detection, and the influence on the detection precision is very little when the detection environment and the detection components are changed.
On the basis of the above embodiment, further referring to fig. 3, in the colloidal gold detection light path, the LED lamp 29 and the PD detection element 30 are respectively connected to the top end of a vertically arranged focusing mirror 20, and the bottom of the focusing mirror 20 is correspondingly provided with a straight mirror 18; referring to fig. 4, in the fluorescence immunoassay optical path, an LED lamp 29 and a PD detection element 30 are respectively connected to the top end of another vertically arranged focusing mirror 20, an optical filter 24 is arranged between the LED lamp 29 and the focusing mirror 20, and the bottom of the focusing mirror 20 is also correspondingly provided with a straight mirror 18; the two in-line mirrors 18 are respectively positioned at the bottom of the detection assembly 5.
Namely, the two detection light paths are separately arranged, each light path is provided with a focusing lens 20, the devices of the two detection light paths are not connected in a cross way, and the two detection light paths are separately operated independently. The LED lamp and the PD detection element in the colloidal gold detection light path are connected with the focusing lens in the colloidal gold detection light path; and the LED lamp and the detection PD element in the fluorescence immunoassay light path are connected with the focusing mirror in the fluorescence immunoassay light path. In order to meet the requirements of focal length, overall dimension, performance parameters and the like, the focusing lens in the embodiment is formed by combining one or more lenses with different types and parameters, comprises one or more groups of lenses, and is formed by packaging.
Referring to fig. 5, the detection principle of fluorescence immunity is that after the LED lamp 29 emits polychromatic light, the light passes through the optical filter 24, and because the bandwidth of the optical filter 24 is relatively narrow, the light obtained here can be regarded as monochromatic light. One part of the monochromatic light is received by the feedback PD element 32 and converted into an electric signal, the other part of the monochromatic light is converged into a point after passing through the focusing mirror 20, then the point is converted into a linear light spot through the linear mirror 18, the monochromatic linear light spot irradiates on a test strip of an object to be tested and generates fluorescence through fluorescence reaction to excite fluorescence, the fluorescence propagates in the opposite direction, one part of the monochromatic linear light spot passes through the linear mirror 18 and the focusing mirror 20 and is received by the detection PD element 30 and converted into an electric signal, the other part of the monochromatic light spot continues to return, and the fluorescence is prevented and further generates refraction and reflection phenomena after encountering the. Since the feedback and detection PD element 30 has received the corresponding signal, the detection result can be obtained through background processing, and the refraction and reflection of the fluorescence returned from the original path have no influence on the detection result, so that no processing is needed.
Even if the machine is calibrated, the detection is carried out immediately, or the detection environment is definitely changed due to the difference of the production batch, the manufacturer, the individual difference and the use condition of the components, and the detection precision is definitely influenced by the change of the detection environment. In order to eliminate or reduce the influence of different detection environments on the detection accuracy, a closed-loop system, namely, a feedback PD element 32 is intentionally added to the detection PD element 30 in the design of the optical path system. By comparing the change conditions of the PD element 32 during calibration and detection in the background, the detection precision can be greatly improved, and the adverse effect on the detection precision caused by the change of the detection environment is not afraid.
Referring to fig. 6, the detection principle of colloidal gold is similar to that of fluorescence immunization, except that no optical filter 24 is provided, after polychromatic light passes through the focusing lens 20 and the linear lens 18, the polychromatic light is absorbed by the test strip of the object to be detected to obtain a spectrum with a corresponding wavelength, and after the spectrums with the other wavelengths return to the original path, the spectrum is received by the PD element 30 to be detected, and a detection result is obtained through background calculation. Because the residual spectrum returned from the original path has no influence on the detection precision, no processing is needed.
Further, in the colloidal gold detection optical path, the LED lamp is connected with a feedback PD element before the focusing mirror. In the fluorescence immunoassay light path, the LED lamp is connected with the feedback PD element after the optical filter and before the focusing mirror.
On the basis of the above embodiment, further, each component in the colloidal gold detection optical path and the fluorescence immunoassay detection optical path are connected by a glass fiber 22. In the colloidal gold detection light path, the LED lamp and the detection PD element are respectively connected with a focusing mirror through glass fibers; in the fluorescence immunoassay light path, one side of the optical filter is arranged towards the LED lamp, and the other side of the optical filter and the detection PD element are respectively connected with the other focusing mirror through glass fibers.
That is, in the colloidal gold detection optical path, the LED lamp 29 is connected to the focusing mirror 20 through the glass fiber 22, the LDE lamp is connected to the feedback PD element 32 through the glass fiber 22, and the detection PD element 30 is connected to the focusing mirror 20 through the glass fiber 22. In the fluorescence immunoassay light path, the optical filter 24 is arranged on one side of the LED lamp 29, the optical filter 24 is connected with the focusing mirror 20, and the focusing mirror 20 is connected with the detection PD element 30 through the glass fiber 22, no connection exists between the LED lamp 29 and the optical filter 24, and the polychromatic light emitted by the LED lamp is directly changed into monochromatic light through the optical filter.
Light is transmitted through the glass fiber 22, the internal structure and the light path design are simplified, the bidirectional light transmission can be realized, the light loss is less, and the light loss can be almost ignored.
On the basis of the above embodiment, further, referring to fig. 2, each component of the colloidal gold detection optical path is embedded and arranged between the first fixing member 15 and the second fixing member 19; the components of the fluorescence immunoassay optical path are embedded between the second fixing member 19 and the third fixing member 26. The mounting is solid construction promptly, and the mounting is inside to set up the recess and is used for placing each device, and the fixed of each device of realization that can be better, the transportation of being convenient for is carried, and guarantees stability, improves and detects the accuracy.
On the basis of the above embodiment, further, referring to fig. 1, two sides of the bottom of the detection assembly 5 are respectively connected with the guide rails 2, the guide rails 2 are arranged on the bottom plate 1, the detection card 7 is placed between the guide rails 2 on two sides, the first end of the guide rail 2 is connected with the front vertical plate 3, the front vertical plate 3 is vertically connected to the bottom plate 1, and the front vertical plate 3 is provided with the card insertion opening.
The detecting component 5 can move along the guide rail 2 to detect different detecting items on the detecting card 7 in sequence. The detection card 7 is inserted between the guide rails 2 at both sides through the insertion opening. The detection card 7 is positioned below the bottom of the detection assembly 5 at a position corresponding to the in-line mirror 18.
Further, the bottom of the fixing member may be provided with a pressing plate 17, and the bottom of the first fixing member 15, the bottom of the second fixing member 19, and the bottom of the third fixing member 26 are respectively connected and fixed with the pressing plate 17. An opening is arranged at the position of the pressure plate 17 corresponding to the straight-line mirror 18. The detection card 7 is positioned below the pressing plate 17, so that light can be conveniently irradiated onto the detection card 7.
On the basis of the above embodiment, further, a rear vertical plate 11 is connected to the second end of the guide rail 2, the rear vertical plate 11 is vertically connected to the bottom plate 1, the detection assembly 5 moves along the guide rail 2 under the driving of a motor, and the motor is fixed to the rear vertical plate 11. The rotation axis of motor can pass back riser 11 and 6 threaded connection of ring flange, and ring flange 6 is fixed in determine module 5 to the operation through the motor can drive determine module 5 and remove along guide rail 2. One side of the motor, which is far away from the rear vertical plate 11, can be provided with an expansion plate 14, the expansion plate 14 is arranged opposite to the rear vertical plate 11, and the expansion plate 14 is connected with the rear vertical plate 11 through a plurality of copper columns 13. A plurality of copper columns 13 are located at the periphery of the motor and play a role of protection with the expansion board 14.
On the basis of the above embodiment, further, two sides of the detecting assembly 5 are respectively provided with a guiding column 8 along the guiding rail 2, and the guiding column 8 penetrates through the detecting assembly 5 and is provided with a bearing sleeve 21 between the guiding column and the detecting assembly 5.
After the detection assembly 5 is fixed with the motor flange 6, the detection assembly moves back and forth through the rotation of a threaded shaft of the stepping motor 12 and the guidance of the guide post 8, and in order to make the movement more stable, a pair of plastic bearing sleeves 21 is specially embedded in the detection assembly 5. In the moving process of the detection assembly 5, the plastic bearing sleeve 21 can generate a self-lubricating effect, reduce friction resistance, improve transmission precision and prolong the service life of the machine.
Specifically, both sides are equipped with the through-hole that runs through respectively on the mounting of determine module 5, and the through-hole is inside to be equipped with bearing housing 21, and guide post 8 passes from bearing housing 21 is inside, is convenient for realize that determine module 5 is smooth along the removal of guide post 8.
On the basis of the above embodiment, further, a matched positioning member is arranged between the detection assembly 5 and the guide rail 2. In order to ensure the accuracy of the moving distance of the detecting component 5, the detecting component 5 is automatically reset by positioning the positioning piece before each detection. The positioning piece can be a groove-shaped optical coupler 28 and an optical coupler baffle plate 10 which are matched. The groove-shaped optical coupler 28 is fixed on the main board 23, and the optical coupler separation blade 10 is fixed on the guide rail 2 or the rear vertical plate 11. The position of the original point is determined by the matching of the groove-shaped optical coupler 28 on the main board 23 and the optical coupler baffle 10, and the moving distance is determined according to a pulse signal sent by the software-controlled stepping motor 12.
On the basis of the above embodiment, further, an elastic element is arranged on the inner wall of the guide rail 2; and a limiting part is arranged on the guide rail 2 and close to the second end. After the detection card 7 is inserted into the bottom of the detection assembly 5 through the card inserting opening, the elastic element is abutted against the detection card 7 on two sides of the detection card 7, so that the detection card 7 is relatively fixed in position, and the detection precision and the consistency are good. The elastic element may be an elastic bead 4, spherical, facilitating smooth insertion of the test card 7.
The analyzer is provided with two hard limit positions in a position which is not far away from the limit moving position of the detection component 5, wherein one hard limit position is that the detection component 5 cannot move forwards continuously after touching the front vertical plate 3, and the other hard limit position is that the detection component 5 touches the limiting part fixed on the guide rail 2, namely that the inner hexagonal socket head screw 9 cannot move backwards continuously. Therefore, the damage of the analyzer to the analyzer due to the failure of the program BUG or the groove-shaped optical coupler 28 and the like can be guaranteed, and the hidden danger of personal safety caused by misoperation of personnel can be avoided.
On the basis of the above embodiment, further, a chromatography analyzer for detecting colloidal gold and fluorescence immunity in the same machine has the specific structure: the LED lamp 29 and the feedback PD element 32 are integrated at corresponding positions on the LED circuit board 27, and the detection PD element 30 is integrated at corresponding positions on the PD circuit board 16. The light transmission is performed through the glass fiber 22, and the design of the glass fiber 22 can not only simplify the internal structure and realize the bidirectional light transmission, but also has higher transmission efficiency than the optical fiber and reduces the loss of the light in the transmission process.
The LED circuit board 27 and the PD circuit board 16 are respectively fixed through screws and fixing pieces, and the corresponding positions inside the first fixing piece 15, the second fixing piece 19 and the third fixing piece 26 are all provided with grooves, so that the LED circuit board 27 and the PD circuit board 16 can be used for fixing the in-line lens 18, the focusing lens 20, the bearing sleeve 21, the glass fiber 22, the optical filter 24, and the LED lamp 29 and the PD element integrated on the LED circuit board 27, and can also play a role in avoiding light. The silica gel pad 25 is arranged in the detection assembly 5, after the LED circuit board 27 and the fixing part are fixed, the silica gel pad 25 is tightly pressed on the surface of the LED circuit board 27 and is just positioned between the LED lamp 29 and the feedback PD element 32 in the LED circuit board 27, the fluorescence immunity and the colloidal gold detection are respectively carried out, and only one item can be detected at the same time, so that the influence on the feedback PD element 32 when the LED lamp 29 emits light can be avoided.
Specifically, when the detection card 7 has both the colloidal gold detection item and the fluorescence immunoassay item, the sequential detection of the two detection items can be realized in one detection process by controlling the conversion of the analyzer. For example, the gold colloidal test items may be arranged on one side of the test card 7, and the immunofluorescent test items may be arranged on the other side of the test card 7. Through the accurate positioning of the detection component 5, the detection component 5 can be moved to the colloidal gold detection item area firstly and then moved to the fluorescence immunoassay item area, the detection component 5 is controlled to operate the colloidal gold detection light path when being positioned in the colloidal gold detection item area, and the fluorescence immunoassay light path when being positioned in the fluorescence immunoassay item area, so that the detection of all detection items on the detection card 7 is realized in one detection process.
The detection card 7 is internally integrated with two types of colloidal gold and fluorescence immunity test strips, each horizontal line on each test strip represents a detection item, and the two types of test strips are respectively arranged in a dot array mode according to different detection items. The detection card 7 can detect various colloidal gold and fluorescence immune items by sampling once, so that the detection efficiency and the detection precision are improved, and the product integration level is higher. In order to further improve the detection precision and the consistency of detection results, glass beads 4 are specially embedded in the bottom plate 1 and the guide rail 2, after the detection card 7 is inserted into the instrument each time, the position of the detection card 7 is relatively fixed under the fixation of the glass beads 4, and the influence of different positions on the detection precision and the consistency of the detection card 7 during each detection is eliminated.
Compared with the prior art, the embodiment has the advantages of simple structure, low price, handheld design, convenience in carrying, small size, convenience in operation and suitability for various different detection environments. Two kinds of test strips of colloidal gold and fluorescence immunity are integrated in the detection card 7, each horizontal line on the test strip represents a detection item, and the test strips are respectively arranged on the two kinds of test strips in a dot array mode according to different detection items. The detection card 7 can detect various colloidal gold and fluorescence immune items by sampling once, so that the detection efficiency and the detection precision are improved, and the product integration level is higher.
The whole analyzer is made of aluminum alloy, and the surface of the analyzer is subjected to black matte treatment by anodic oxidation, so that the analyzer is light in weight, attractive in appearance and corrosion-resistant, and can also play a role in keeping out of the sun on a light path.
The analyzer of the embodiment has the advantages of simple structure, low price, handheld design, convenience in carrying, small size, convenience in operation and suitability for various different detection environments. Light is transmitted through the glass fiber 22, the internal structure and the design of an optical path are simplified, the bidirectional light transmission can be realized, and the light loss can be almost ignored. Two kinds of test strips of colloidal gold and fluorescence immunity are integrated in the detection card 7, each horizontal line on the test strip represents a detection item, and the test strips are respectively arranged on the two kinds of test strips in a dot array mode according to different detection items. The detection card 7 can detect various colloidal gold and fluorescence immune items by sampling once, so that the detection efficiency and the detection precision are improved, and the product integration level is higher. After the detection card 7 is inserted into the instrument, the position is relatively fixed, the detection precision and consistency are better, the whole machine is processed by adopting an aluminum alloy material, the surface is subjected to black matte treatment by anodic oxidation, and the light-shading integrated detection device is light in weight, attractive in appearance, corrosion-resistant and capable of shading a light path.
The above description is only a preferred embodiment of the present invention, and should not be taken as limiting the invention, and any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (10)
1. The chromatography analyzer for simultaneously detecting colloidal gold and fluorescence immunity is characterized by comprising a detection assembly, wherein the detection assembly comprises a fixing piece, an LED circuit board, a PD circuit board and a mainboard;
the fixing parts comprise a first fixing part, a second fixing part and a third fixing part which are sequentially arranged in parallel and are respectively vertical to the LED circuit board, a colloidal gold detection light path is arranged between the first fixing part and the second fixing part, and a fluorescence immunoassay detection light path is arranged between the second fixing part and the third fixing part;
the LED circuit board is provided with two LED lamps correspondingly connected with the colloidal gold detection light path and the fluorescence immunoassay detection light path, the PD circuit board is provided with two detection PD elements correspondingly connected with the colloidal gold detection light path and the fluorescence immunoassay detection light path, and the LED circuit board and the PD circuit board are respectively connected with the mainboard.
2. The chromatography analyzer for detecting colloidal gold and fluorescence immunity in the same machine as in claim 1, wherein the LED circuit board is further provided with two feedback PD elements, the LED lamp connected to the colloidal gold detection optical path is simultaneously connected to one feedback PD element, and the LED lamp connected to the fluorescence immunity detection optical path is also simultaneously connected to one feedback PD element.
3. The chromatography analyzer for in-line detection of colloidal gold and fluorescence immunity according to claim 1, wherein in the colloidal gold detection light path, the LED lamp and the PD detection element are respectively connected to the top end of a vertically disposed focusing mirror, and the bottom of the focusing mirror is correspondingly provided with a linear mirror;
in the fluorescence immunoassay light path, an LED lamp and a detection PD element are respectively connected with the top end of another vertically arranged focusing mirror, an optical filter is arranged between the LED lamp and the focusing mirror, and the bottom of the focusing mirror is also correspondingly provided with a straight mirror;
the two linear mirrors are respectively positioned at the bottom of the detection assembly.
4. The chromatography analyzer for in-situ detection of colloidal gold and fluorescence immunoassay according to claim 3, wherein in the colloidal gold detection light path, the LED lamp and the detection PD element are respectively connected with a focusing mirror through glass fibers;
in the fluorescence immunoassay light path, one side of the optical filter is arranged towards the LED lamp, and the other side of the optical filter and the detection PD element are respectively connected with the other focusing mirror through glass fibers.
5. The chromatography analyzer for in-line detection of colloidal gold and fluorescence immunoassay according to claim 1, wherein each component of the colloidal gold detection optical path is embedded between a first fixing member and the second fixing member; and each part of the fluorescence immunoassay light path is embedded between the second fixing piece and the third fixing piece.
6. The chromatography analyzer for detecting colloidal gold and fluorescence immunity in the same machine as in any one of claims 1 to 5, wherein two sides of the bottom of the detection assembly are respectively connected with a guide rail, the guide rail is arranged on a bottom plate, a detection card is arranged between the guide rails at the two sides, a first end of the guide rail is connected with a front vertical plate, the front vertical plate is vertically connected with the bottom plate, and a card inserting opening is arranged on the front vertical plate.
7. The chromatography analyzer for detecting colloidal gold and fluorescence immunity in the same machine as in claim 6, wherein a second end of the guide rail is connected with a rear vertical plate, the rear vertical plate is vertically connected to the bottom plate, the detection assembly moves along the guide rail under the driving of a motor, and the motor is fixed on the rear vertical plate.
8. The chromatography analyzer for simultaneous detection of colloidal gold and fluorescence immunity according to claim 6, wherein guide posts are respectively disposed along the guide rail at two sides of the detection assembly, the guide posts penetrate through the detection assembly, and a bearing sleeve is disposed between the guide posts and the detection assembly.
9. The chromatography analyzer for simultaneous detection of colloidal gold and fluorescence immunity according to claim 6, wherein a matching positioning member is provided between the detection assembly and the guide rail.
10. The chromatography analyzer for simultaneous detection of colloidal gold and fluorescence immunity according to claim 6, wherein an elastic member is provided on an inner wall of the guide rail; and a limiting part is arranged on the guide rail close to the second end.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
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