CN112033882A - Method and device for testing microbial corrosion of ocean tidal zone - Google Patents
Method and device for testing microbial corrosion of ocean tidal zone Download PDFInfo
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- CN112033882A CN112033882A CN202010909129.XA CN202010909129A CN112033882A CN 112033882 A CN112033882 A CN 112033882A CN 202010909129 A CN202010909129 A CN 202010909129A CN 112033882 A CN112033882 A CN 112033882A
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- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N17/00—Investigating resistance of materials to the weather, to corrosion, or to light
- G01N17/006—Investigating resistance of materials to the weather, to corrosion, or to light of metals
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N17/00—Investigating resistance of materials to the weather, to corrosion, or to light
- G01N17/02—Electrochemical measuring systems for weathering, corrosion or corrosion-protection measurement
Abstract
The invention discloses a method and a device for testing microbial corrosion in a marine tidal zone. The test device comprises a base, a bracket, a brine tank, a water pump, a fan and a control box; the cover plate at the top of the brine pool is provided with an ultrasonic liquid level sensor, a blowing port, a wind speed sensor, a gas exchange hole, a microorganism inoculation hole and a test hole, and the bottom of the brine pool is provided with a test plate, a wave making pump, a flow speed sensor, a heating rod and a temperature sensor. The invention integrates the functions of tidal form control, environmental temperature regulation, ocean current and sea wind reproduction, microorganism inoculation, test and the like through the singlechip, realizes the online monitoring of the metal material corrosion process in the ocean tidal zone through the external electrochemical workstation, has simple structure and convenient operation, and is suitable for the experimental research of the microorganism corrosion of the metal material in the ocean tidal zone.
Description
Technical Field
The invention belongs to the field of marine environment material durability equipment, and particularly relates to a method and a device for testing microbial corrosion in a marine tidal region, which are used for accelerated corrosion test research on long-term corrosion resistance of a marine platform and a marine component.
Background
The ocean reserves abundant resources and has potential huge economic benefits and strategic national defense status. The development and utilization of marine resources cannot be separated from offshore infrastructure, and the problem of marine corrosion is one of the important threats faced by the offshore infrastructure. Huge manpower and material resources are invested in all countries in the world to research the marine corrosion problem. Recently, the China science society establishes the corrosion mechanism of metal materials under the action of offshore pollutants as one of the ten-ocean front-end problems and engineering technical problems in 2020. Therefore, the problem of corrosion of offshore metal materials becomes a technical problem restricting the marine science of China. In order to simulate and accelerate the corrosion process of the metal material in a laboratory and reveal the corrosion mechanism of the metal material in the marine environment, a method for simulating the marine environment needs to be developed and corresponding equipment needs to be developed. One important device for simulating the corrosion failure of metal materials near a coastline is a marine tidal corrosion device. As known in research, the existing feasible methods and special equipment for simulating ocean tidal corrosion are few, and the functions are not complete. For example, some devices are only used for demonstration of ocean tides, are mainly used for appreciation and teaching, and have no scientific research significance; some devices only consider the influence of the tide fluctuation on the metal corrosion behavior, and have limited application; some equipment can only carry out simple soaking tests, cannot carry out online monitoring and detection, cannot consider the effects of factors such as marine physics, chemistry, biology and the like, and cannot be used for exploring a corrosion mechanism; most devices artificially set the tidal fluctuation to be linear, which is not consistent with the tidal fluctuation law in nature, and cannot truly simulate the influence of ocean tides on the metal corrosion mechanism. Therefore, for revealing the corrosion mechanism of metallic materials under the action of offshore pollutants, the development of test methods and devices suitable for metallic material corrosion and microbial corrosion in the offshore environment is undoubtedly used for the problem to be solved. Aiming at the defects of the prior art, the invention provides a method and a device for testing microbial corrosion in a marine tidal region from the aspects of tidal morphology, online monitoring, marine physical, chemical and biological factors and the like.
Disclosure of Invention
The invention provides a method and a device for testing microbial corrosion in a marine tidal zone, which have the advantages of simple structure, reasonable price and good stability, and aims at solving the problems of improper tidal form, difficult on-line monitoring and poor consideration of marine physical, chemical and biological factors in the method and the device for testing the metal material corrosion and the microbial corrosion in the marine tidal zone.
The invention relates to a test device for microbial corrosion in a marine tidal zone, which comprises a base, a brine pond, a tidal control system, an environment control system and an online monitoring system. The base top be provided with support, first salt pond and second salt pond, supreme first water pump and the second water pump, first fan and second fan, the control box of having set gradually are followed to the support, first salt pond and second salt pond distribute on the support both sides.
A tide control unit and an environment control unit are integrated in the control box; the control box in be provided with ATmeqa328 singlechip, 12V power adapter and cooling fan etc. the singlechip program is compiled through Arduino 1.8.3 software package, writes into through Arduino UNO R3 development board. The simulation of the tidal morphology and the adjustment of physical, biological and chemical parameters of the ocean can be realized.
The first brine tank and the second brine tank are both internally and externally made of 316L stainless steel plates with the thickness of 3mm, and hard foamed urethane is adopted in the middle for keeping the temperature of seawater; a first liquid level pipe and a second liquid level pipe which are connected with the inside of the brine pond are respectively arranged outside the first brine pond and the second brine pond and are used for displaying the liquid level height in the brine pond; the top parts of the first brine pool and the second brine pool are respectively provided with a first cover plate and a second cover plate, the first cover plate is provided with a first ultrasonic liquid level sensor, a first air blowing opening, a first air speed sensor, a first gas exchange hole and a microorganism inoculation and test hole, and the second cover plate is provided with a second ultrasonic liquid level sensor, a second air blowing opening, a second air speed sensor and a second gas exchange hole; the bottom of the first salt water pool and the bottom of the second salt water pool are respectively provided with a first test board, a first wave making pump, a first flow velocity sensor, a first heating rod, a first temperature sensor, a second test board, a second wave making pump, a second flow velocity sensor, a second heating rod and a second temperature sensor. The first ultrasonic liquid level sensor is connected with the control box through a signal line and used for measuring the liquid level height in the first saline pool, and the second ultrasonic liquid level sensor is connected with the control box through a signal line and used for measuring the liquid level height in the second saline pool; the first gas exchange hole and the second gas exchange hole are plugged by breathable silica gel in the using process, are used for gas exchange, and simultaneously prevent external microbial pollution; the microorganism inoculation and test hole is connected with a capillary, the capillary extends into the middle of the box body and is used for inoculating microorganisms and testing the quantity of the microorganisms, the microorganism inoculation and test hole is in a closed state in the test process, and the capillary is temporarily opened during inoculation and test.
The tide control system comprises a tide control unit, a first ultrasonic liquid level sensor, a second ultrasonic liquid level sensor, a first water pump, a second water pump, a first electromagnetic valve, a second electromagnetic valve, a first water guide pipe, a second water guide pipe, a third water guide pipe and a fourth water guide pipe in a control box; the first ultrasonic liquid level sensor is connected with the control box through a signal line and is used for transmitting liquid level height data of the first saline pond; the second ultrasonic liquid level sensor is connected with the control box through a signal line and is used for transmitting liquid level height data of the second brine pool; the first water guide pipe is connected with the bottom of the first brine tank in a penetrating way and is connected with the first water pump through a first electromagnetic valve, and the second water guide pipe is connected with the top of the second brine tank in a penetrating way and is directly connected with the first water pump; the third water guide pipe is connected with the bottom of the second brine pool in a penetrating way and is connected with the second water pump through a second electromagnetic valve, and the fourth water guide pipe is connected with the top of the first brine pool in a penetrating way and is directly connected with the second water pump; the tidal control unit controls the voltage of the first water pump and the second water pump so as to control the water flow speed; the first electromagnetic valve and the second electromagnetic valve are both connected with the control box, and the tide unit controls the opening and closing of the first electromagnetic valve and the second electromagnetic valve; the tide control unit can adjust the voltage of the first water pump and the second water pump according to the setting of a user, control the liquid level height change rule of the first brine pool and the second brine pool, and form the tide rule of sine, triangle and trapezoid fluctuation.
The environment control system comprises a first fan, a second fan, a first wind speed sensor, a second wind speed sensor, a first wave making pump, a second wave making pump, a first flow speed sensor, a second flow speed sensor, a first heating rod, a second heating rod, a first temperature sensor, a second temperature sensor and an environment control unit in a control box. The environment control unit respectively controls the rotating speeds of the first fan and the second fan and receives the wind speed data of the first wind speed sensor and the second wind speed sensor; the first wave making pump, the second wave making pump, the first flow sensor and the second flow sensor are connected with the control box, and the environment control unit controls the rotating speeds of the first wave making pump and the second wave making pump and receives water flow data of the first water flow sensor and the second water flow sensor; the first heating rod, the second heating rod, the first temperature sensor and the second temperature sensor are connected with the control box, and the environment control unit controls the voltage of the first heating rod and the voltage of the second heating pump and receives temperature data of the first temperature sensor and the second temperature sensor. The environment system can receive wind speed data from the first wind speed sensor and the second wind speed sensor, flow speed data from the first water flow sensor and the second water flow sensor and temperature data from the first temperature sensor and the second temperature sensor according to user setting, and respectively control the rotating speeds of the first fan and the second fan, the rotating speeds of the first wave making pump and the second wave making pump and the voltages of the first heating rod and the second heating rod, so that sea wave, sea wind and temperature environments set by users are formed.
The online monitoring system comprises a first test board, a second test board and an external electrochemical test system; the first test board and the second test board are respectively provided with a first test sample, a first auxiliary electrode, a first reference electrode, a second test sample, a second auxiliary electrode and a second reference electrode; the first test sample, the first auxiliary electrode, the first reference electrode, the second test sample, the second auxiliary electrode and the second reference electrode are all connected with leads, and the leads penetrate through the first salt water pool and the second salt water pool and are connected with an external electrochemical test system; the external electrochemical test system is common equipment for a metal material corrosion laboratory, and is not in the scope of the invention; the online monitoring system can monitor the corrosion degree and the process of samples at different positions of the ocean tidal zone in real time.
The invention also provides a test method for the microbial corrosion of the marine tidal zone, which has high automation degree and simple operation, can scientifically and reasonably evaluate the microbial corrosion characteristics of the metal materials in the marine tidal zone, and can provide technical support for the research on the microbial corrosion behavior and mechanism of the metal materials in the marine tidal environment.
A method for testing microbial corrosion in a tidal region of the ocean comprises the following steps.
The method comprises the following steps: preparing strains and seawater: selecting and preparing a culture medium according to strains, and culturing the strains in the culture medium; taking clean seawater from the ocean, and filtering by using filter paper to remove impurities in the seawater; the culture medium, seawater and utensils are sterilized by high-temperature steam, so as to prevent contamination of bacteria.
Step two: preparation of a sample to be tested and an electrode: a first test plate and a second test plate were prepared as shown in FIG. 1, a test sample, an auxiliary electrode and a reference electrode were mounted, and the first test plate and the second test plate were mounted in the test device as shown in FIG. 1.
Step three: sterilizing a test device: the test apparatus was placed in a room where ultraviolet sterilization was possible, and ultraviolet sterilization was performed.
Step four: sea water filling and microbial inoculation: slowly injecting sterilized seawater into the first salt water pool until the water level just passes through the top end of the first test board, and injecting sterilized seawater into the second salt water pool until the water level just passes through the second temperature sensor; and plugging the first gas exchange hole and the second gas exchange hole by using the gas-permeable silica gel, and inoculating microorganisms through the microorganism inoculation hole and the test hole.
Step five: tidal and environmental parameter control: connecting the control box with a computer, starting a corresponding execution program, inputting parameters such as tidal cycle and form, ambient temperature, seawater flow velocity and sea wind velocity, and starting the program.
Step six: online monitoring: and connecting the first test sample, the first auxiliary electrode, the first reference electrode, the second test sample, the second auxiliary electrode and the second reference electrode with an external electrochemical test system through leads, and starting to monitor the microbial corrosion process and behavior of the sample in the ocean tidal zone.
The strain in the step one can be separated from natural seawater or purchased from a national microorganism preservation center; different liquid culture mediums are adopted for different strains, and a common liquid formula in the prior art is recommended to be selected; in order to reduce the influence of the culture medium on the test result, the inoculation amount is less than or equal to one thousandth of the volume of the seawater; the seawater can be natural seawater from natural seawater, or 3.5% NaCl or seawater crystal.
And in the second step, the specification of the sample to be detected is a phi 10mm wafer, the auxiliary electrode is a commercial graphite sheet, and the reference electrode is a commercial saturated calomel electrode which is connected through a self-made salt bridge.
In the fourth step, the microorganism inoculation is carried out through microorganism inoculation and a test hole, the hole can also be used for counting microorganisms in seawater in the microorganism corrosion process, and the counting technology is carried out by adopting the existing method. The microorganism inoculation and test hole is in a closed state during the test process, and is temporarily opened during the inoculation and test.
And fifthly, the functions of tidal form control, environmental temperature regulation, ocean current and sea wind reproduction, microorganism culture and test and the like are realized through the tidal control system, the environmental control system and the online monitoring system, and the functions are integrated in the control box and are controlled and regulated through software.
The external electrochemical test system in the sixth step is an electrochemical workstation commonly used in corrosion and electrochemical laboratories, and can perform electrochemical tests such as open-circuit potential, electrochemical impedance spectroscopy, polarization curves, cyclic polarization and the like, and the external lead of the electrode is prepared in the second step.
Compared with the prior art and the method, the method has the following beneficial effects.
1. According to the invention, through a tide control system consisting of a tide control unit, an ultrasonic liquid level sensor, a water pump and the like, the liquid level of the brine pool can be controlled according to the requirements of users, so that sine, triangle and trapezoid tide rhythms are formed, wherein the sine is closer to the tide rhythm in the natural environment, and the research on the metal material tide corrosion mechanism is more accurate and scientific.
2. The invention controls the temperature of the marine environment, sea waves and sea wind through the environment control system, can simulate the tidal corrosion behavior of metal materials in natural environment, and is more real and reliable.
3. The invention detects the corrosion process of the metal material through the on-line monitoring system, and is beneficial to disclosing the corrosion mechanism of the metal material in the ocean tidal region.
4. The invention is provided with the gas exchange hole and the microorganism inoculation and test hole, can finish the microorganism corrosion of the metal material in the ocean area and can prevent the microorganism pollution.
5. The invention can realize the full-true simulation of parameters in aspects of tidal morphology, marine physics, chemistry, biology and the like in the natural marine environment through the integrated control box and the single chip microcomputer for centralized control, and can truly reproduce the metal material corrosion environment in the marine tidal region.
Drawings
FIG. 1 is a schematic diagram of the present invention.
Figure 2 inoculation and sterilization of corrosion rates in carbon steel ocean tidal zones in seawater.
FIG. 3 electrochemical impedance spectra of carbon steel in ocean tidal zones in seeded and sterilized seawater.
Detailed Description
The invention will be further described with reference to the accompanying drawings.
Referring to fig. 1, a support 8, a first brine pond 2 and a second brine pond 16 are arranged above a base 1 of the testing device for microbial corrosion in a marine tidal zone, and the support is sequentially provided with a first water pump 9, a second water pump 11, a first fan 7, a second fan 10 and a control box 32 from bottom to top. A first liquid level pipe 45 and a second liquid level pipe 20 which are connected with the inside of the brine tank are respectively arranged outside the first brine tank 2 and the second brine tank 16; the top parts of the first brine pool 2 and the second brine pool 16 are respectively provided with a first cover plate 41 and a second cover plate 28, the first cover plate 41 is provided with a first ultrasonic liquid level sensor 40, a first air blowing opening 38, a first air speed sensor 39, a first gas exchange hole 37 and a microorganism inoculation and test hole 36, and the second cover plate 28 is provided with a second ultrasonic liquid level sensor 27, a second air blowing opening 26, a second air speed sensor 25 and a second gas exchange hole 29; the first test board 3, the first wave making pump 4, the first flow sensor 48, the first heating rod 49, the first temperature sensor 47, the second test board 17, the second wave making pump 15, the second flow sensor 19, the second heating rod 18 and the second temperature sensor 21 are respectively arranged at the bottoms of the first brine pool 2 and the second brine pool 16. The first air guide pipe 33 penetrates through the first cover plate 41 to be connected with the first air blowing opening, and the other end of the first air guide pipe is connected with the first fan 7; the second air duct 30 penetrates through the second cover plate 28 and is connected with the second air blowing port 26, and the other end of the second air duct is connected with the second fan 10. The first water guide pipe 5 is connected with the bottom of the first brine tank 2 in a penetrating way and is connected with the first water pump 9 through the first electromagnetic valve 6, and the second water guide pipe 12 is connected with the top of the second brine tank 16 in a penetrating way and is directly connected with the first water pump 9; the third water conduit 14 is connected with the bottom of the second brine tank 16 in a penetrating way and is connected with the second water pump 11 through the second electromagnetic valve 13, and the fourth water conduit 34 is connected with the top of the first brine tank 2 in a penetrating way and is directly connected with the second water pump 11.
In this embodiment, it is preferable that the microorganism inoculation and test well 36 is connected with a capillary 35; the first cover plate 41 and the second cover plate 28 are provided with a first gas exchange hole 37 and a second gas exchange hole 29, respectively. Can realize the inoculation, the culture and the test of microorganisms, and is suitable for the research of the microbial corrosion in the marine tidal zone of metal materials.
In this embodiment, preferably, the first water pump 9, the second water pump 11, the first electromagnetic valve 6, the second electromagnetic valve 13, the first fan 7, the second fan 10, the first wave generating pump 4, and the second wave generating pump 15 are all connected to the control box 32. The first wind speed sensor 39, the second wind speed sensor 25, the first flow rate sensor 48, the second flow rate sensor 19, the first heating rod 49, the second heating rod 18, the first temperature sensor 47, the second temperature sensor 21, the first ultrasonic liquid level sensor 40 and the second ultrasonic liquid level sensor 27 are all connected with the control box 32 through signal lines 31. The simulation of the tide form and the individual adjustment of physical, biological and chemical parameters of the ocean can be realized.
In the present embodiment, it is preferable that the first test board 3, the second test board 17 are respectively provided with a first test sample 42, a first auxiliary electrode 44, a first reference electrode 43, and a second test sample 24, a second auxiliary electrode 22, a second reference electrode 23; the first test sample 42, the first auxiliary electrode 44, the first reference electrode 43, the second test sample 24, the second auxiliary electrode 22 and the second reference electrode 23 are all connected with an external electrochemical test system through wires penetrating through the first saline reservoir 2 and the second saline reservoir 16. The online monitoring of the corrosion degree and the process of the metal material can be realized.
The invention relates to a method for testing microbial corrosion in a tidal zone of a sea.
The method comprises the following steps: preparing strains and seawater: selecting and preparing a culture medium, and culturing strains; taking clean seawater, and filtering to remove impurities in the seawater; the culture medium, the seawater and the vessels are sterilized by high-pressure steam, and the preferred conditions of the high-pressure steam sterilization are as follows: 103kPa, 121 ℃ and the time length is more than 21min, and the following are similar.
The seawater can be natural seawater taken from natural seawater, and can also be replaced by 3.5% NaCl or seawater crystal, and is also within the protection scope of the invention.
Step two: preparation of a sample to be tested and an electrode: a first test board 3 and a second test board 17 were prepared, a first test sample 42, a second test sample 24, a first auxiliary electrode 44, a second auxiliary electrode 22, a first reference electrode 43 and a second reference electrode 23 were arranged, and the first test board 3 and the second test board 17 were arranged in the test device as shown in fig. 1. The specification of the test sample is a phi 10mm wafer, the auxiliary electrode is a commercial graphite sheet, and the reference electrode is a commercial saturated calomel electrode which is connected through a self-made salt bridge.
Alternatively, the test specimens may be of one or more specifications, and may even be positioned outside of first test panel 3 and second test panel 17 for weight loss analysis, within the scope of the present invention.
Step three: sterilizing a test device: the test apparatus was placed in a room where ultraviolet sterilization was possible, and ultraviolet sterilization was performed. The preferable conditions of ultraviolet sterilization are as follows: the negative pressure sterilization room is provided with a buffer space, and the sterilization time is 2 hours. After sterilization, operators need to wear sterile working clothes for subsequent operation.
Step four: sea water filling and microbial inoculation: slowly injecting sterilized seawater 46 into the first salt water pool 2 until the water level just submerges the top end of the first test board 3, and injecting sterilized seawater into the second salt water pool 16 until the water level just submerges the second temperature sensor 21; the first gas exchange hole 37 and the second gas exchange hole 29 are blocked by using gas-permeable silica gel, microorganisms are inoculated through the microorganism inoculation and test hole 36, the holes are sealed after the inoculation is finished, and the same amount of culture medium is added in the control group experiment.
Step five: tidal and environmental parameter control: the control box 32 is connected to a computer, corresponding execution programs are started, parameters such as tidal cycle and form, ambient temperature, seawater flow rate and sea wind speed are input, and the programs are started.
The tide form can be selected from three forms of sine, triangle and trapezoid, the tide cycle can be controlled within the range of 1-24 h, the ambient temperature range is normal water temperature (about 20 ℃) to 45 ℃, the seawater flow speed range is 0-1 m/s, and the seawater speed range is 0-10 m/s.
Step six: online monitoring: and (3) connecting the first test sample 42, the first auxiliary electrode 44, the first reference electrode 43, the second test sample 24, the second auxiliary electrode 22 and the second reference electrode 23 with an external electrochemical test system through leads to start monitoring the microbial corrosion process and behavior of the samples in the ocean tidal region. The microorganisms are counted at intervals, and the counting technique is carried out by the existing method.
The monitoring method in the sixth step comprises electrochemical tests such as open-circuit potential, electrochemical impedance spectrum, polarization curve, circular polarization and the like. And preparing an external lead of the electrode in the second step.
The above examples are merely illustrative for clearly illustrating the present invention and are not intended to limit the embodiments. Other variations and modifications will be apparent to persons skilled in the art in light of the above description. Nor is it intended to be exhaustive of all embodiments. And obvious variations or modifications of the invention may be made without departing from the scope of the invention.
The results of the iron bacterial corrosion test of the carbon steel in the tidal zone of the ocean are as follows:
the bacteria inoculated in the above examples are iron bacteria, the tide morphology is sinusoidal, the tide cycle is 12h, and the experimental duration is 60 weeks. The experimental samples were placed in the full immersion zone, the low tide zone, the medium tide zone and the high tide zone, respectively, as shown in fig. 1. Monitoring electrochemical impedance spectrum, polarization curve and linear polarization resistance at intervals in the experiment process, and representing the average corrosion rate by corrosion weightlessness after the experiment is finished. Fig. 2 lists the electrochemical impedance spectra of the samples in the full immersion area and the high-tide area at week 60, and it can be seen that the corrosion rate of the sample in the high-tide area is much higher than that of the sample in the full immersion area in the same seawater environment, and the corrosion rate of the sample in the high-tide area is accelerated but reduced by the presence of iron bacteria. Figure 3 lists the average corrosion rate of the samples after 60 cycles of tidal cycling, and clearly the results for the corrosion rate are consistent with the electrochemical impedance spectroscopy results.
Preferred embodiments of the present invention have been described in detail by way of the above examples, but the present invention is not limited to the details of the above embodiments, and various equivalent changes can be made to the technique of the present invention within the technical spirit of the present invention, and these equivalent changes are within the scope of the present invention.
Claims (8)
1. A test method for microbial corrosion in a marine tidal zone is characterized by comprising the following steps: the method comprises the following steps:
step one, strain and seawater preparation: selecting and preparing a proper culture medium for strain culture; taking clean seawater from the ocean, and filtering to remove impurities in the seawater; sterilizing the culture medium, seawater and utensils with high temperature steam;
step two, preparing a sample to be detected and an electrode: preparing a test board, arranging a test sample, an auxiliary electrode and a reference electrode, and arranging the test board into a test device;
step three, sterilizing the test device: placing the test device in a room capable of being subjected to ultraviolet sterilization to perform ultraviolet sterilization;
step four, seawater filling and microorganism inoculation: injecting proper amount of sterilized seawater into the brine pond; plugging the gas exchange hole with gas-permeable silica gel, and inoculating microorganisms through the microorganism inoculation and test hole;
step five, tide and environment parameter control: connecting the control box with a computer, starting a corresponding execution program, inputting parameters such as tidal cycle and form, ambient temperature, seawater flow velocity and sea wind velocity, and starting the program;
step six, online monitoring: connecting the test sample, the auxiliary electrode and the reference electrode with an external electrochemical test system through leads, and starting to monitor the microbial corrosion process and behavior of the sample in the ocean tide region; the microorganisms are counted at intervals, and the counting technique is carried out by the existing method.
2. The method of testing for microbial corrosion in a tidal zone of the ocean of claim 1, wherein: the strain can be separated from natural seawater or purchased from national microorganism preservation center, and the recommended inoculation amount is less than or equal to one thousandth of the volume of the seawater; the seawater can be natural seawater from natural sea area, or 3.5% NaCl or seawater crystal.
3. The method of testing for microbial corrosion in a tidal zone of the ocean of claim 1, wherein: the specification of the sample to be tested is a phi 10mm wafer, the auxiliary electrode is a commercial graphite sheet, and the reference electrode is a commercial saturated calomel electrode and is connected through a self-made salt bridge; the test plate can be one or more specifications and can even be arranged outside the test plate for weightlessness analysis.
4. A test device for microbial corrosion in a marine tidal zone, which is based on the test method for microbial corrosion in the marine tidal zone as claimed in any one of claims 1 to 3, and is characterized in that: a bracket and a brine tank are arranged above the base, and the bracket is sequentially provided with a water pump, a fan and a control box from bottom to top; the top of the saline water pool is respectively provided with a cover plate, the cover plate is provided with an ultrasonic liquid level sensor, a blowing port, a wind speed sensor, a gas exchange hole, a microorganism inoculation hole and a test hole, and the bottom of the saline water pool is respectively provided with a test plate, a wave making pump, a flow speed sensor, a heating rod and a temperature sensor; the guide duct is connected with the blowing port, the cover plate and the fan, and the guide duct is connected with the brine pool, the electromagnetic valve and the water pump.
5. The apparatus for testing microbial corrosion in a tidal zone of the ocean of claim 4, wherein: the cover plate is provided with a gas exchange hole, a microorganism inoculation hole and a test hole, and the lower end of the microorganism inoculation hole and the lower end of the test hole are connected with a capillary tube; the microbial inoculation, culture and test in the process of ocean tidal rhythm can be realized; the counting technology is carried out by adopting the existing method, the microorganism inoculation and the test hole are in a closed state in the test process, and the microorganism inoculation and the test hole are temporarily opened during the inoculation and the test.
6. The apparatus for testing microbial corrosion in a tidal zone of the ocean of claim 4, wherein: the tide control system is composed of the control box, the ultrasonic liquid level sensor, the water pump, the electromagnetic valve and the like, the liquid level height of the brine pool can be controlled according to setting, three tide rhythms of sine, triangle, trapezoid and the like are formed, and the tide period can be controlled within the range of 1-24 h.
7. The apparatus for testing microbial corrosion in a tidal zone of the ocean of claim 4, wherein: the environment control system consisting of the control box, the fan, the blowing port, the wind speed sensor, the wave making pump, the flow velocity sensor, the heating rod, the temperature sensor and the like can control parameters such as environment temperature, seawater flow velocity, seawater speed and the like according to setting; the normal water temperature (about 20 ℃) in the environment temperature range is 45 ℃, the flow speed of the seawater is 0-1 m/s, and the speed of the sea wind is 0-10 m/s; the control box integrates the control of tide form, environment temperature, seawater flow velocity, sea wind velocity and the like, and realizes the computer control of test parameters.
8. The apparatus for testing microbial corrosion in a tidal zone of the ocean of claim 4, wherein: the test board is provided with a test sample, an auxiliary electrode and a reference electrode, can be externally connected with an electrochemical test system, and can realize the online monitoring of the corrosion degree and the process of metal materials in the ocean tide process; the external electrochemical test system is an electrochemical workstation commonly used in corrosion and electrochemical laboratories, and can perform electrochemical tests such as open-circuit potential, electrochemical impedance spectroscopy, polarization curves, cyclic polarization and the like.
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| CN112964628A (en) * | 2021-02-03 | 2021-06-15 | 中山大学 | Research method of microbial corrosion electron transfer mechanism and application thereof |
| CN113029930A (en) * | 2021-03-16 | 2021-06-25 | 中国船舶重工集团公司第七二五研究所 | Corrosion test method for marine fouling organisms in real sea environment |
| CN114088613A (en) * | 2021-10-22 | 2022-02-25 | 武汉科思特仪器股份有限公司 | Tidal environment metal corrosion monitoring device and testing method |
| CN114397239A (en) * | 2021-12-15 | 2022-04-26 | 国核电站运行服务技术有限公司 | Device for on-line monitoring of microorganisms in fluid |
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