CN112022754A - Skin care composition for whitening and moisturizing and preparation method and application thereof - Google Patents

Skin care composition for whitening and moisturizing and preparation method and application thereof Download PDF

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CN112022754A
CN112022754A CN202011112008.9A CN202011112008A CN112022754A CN 112022754 A CN112022754 A CN 112022754A CN 202011112008 A CN202011112008 A CN 202011112008A CN 112022754 A CN112022754 A CN 112022754A
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梁其文
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    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00Cosmetics or similar toiletry preparations
    • A61K8/18Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A61K8/9706Algae
    • A61K8/9717Rhodophycota or Rhodophyta [red algae], e.g. Porphyra
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00Cosmetics or similar toiletry preparations
    • A61K8/18Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A61K8/9783Angiosperms [Magnoliophyta]
    • A61K8/9789Magnoliopsida [dicotyledons]
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00Cosmetics or similar toiletry preparations
    • A61K8/18Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A61K8/9783Angiosperms [Magnoliophyta]
    • A61K8/9794Liliopsida [monocotyledons]
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61QSPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00Preparations for care of the skin
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61QSPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00Preparations for care of the skin
    • A61Q19/02Preparations for care of the skin for chemically bleaching or whitening the skin
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61QSPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00Preparations for care of the skin
    • A61Q19/08Anti-ageing preparations
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/40Chemical, physico-chemical or functional or structural properties of particular ingredients
    • A61K2800/59Mixtures
    • A61K2800/592Mixtures of compounds complementing their respective functions
    • A61K2800/5922At least two compounds being classified in the same subclass of A61K8/18

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Abstract

The invention belongs to the technical field of cosmetics, and particularly relates to a skin care composition for whitening and moisturizing, and a preparation method and application thereof, wherein the skin care composition comprises the following components in parts by weight: extracts of arachnoid bluegrass, Chondrus crispus, Craimum maritimum and Hippophae rhamnoides. The skin care composition can repair internal inflammation of skin and fade pigment of a dermis layer, thereby achieving the purpose of effectively lightening the pigmentation after facial inflammation, preventing skin aging and enabling the skin to be white and fine; in addition, the skin care composition can enhance the strength and the appearance of the corneocytes and enable the shape of the corneocytes to be more complete and compact through the combined action of the four effective components, thereby effectively avoiding water loss in a low-humidity environment and improving the water content of the skin from the source; meanwhile, the moisture in the environment can be absorbed to the maximum extent, and the instant and lasting moisturizing effect in a dry environment is realized through water capture, water locking and water storage.

Description

Skin care composition for whitening and moisturizing and preparation method and application thereof
Technical Field
The invention belongs to the technical field of cosmetics, and particularly relates to a skin care composition for whitening and moisturizing, and a preparation method and application thereof.
Background
The moisture content of the skin in the human body is 15-20% of the body weight, and if the moisture content of the skin is reduced, the skin is dry, dull, low in elasticity and much in wrinkles. Currently, the commonly used moisturizers of cosmetics are mainly divided into closed moisturizers, hygroscopic moisturizers and hydration moisturizers, wherein the closed moisturizers achieve the moisturizing effect by preventing moisture in skin from evaporating, and the hygroscopic moisturizers achieve the moisturizing effect by absorbing moisture in the external environment and supplementing the moisture into the stratum corneum; the hydration-type moisturizer needs a substance which is combined with water molecules in the environment to form a network structure so as to achieve the effect of supplementing water for the skin. But when a human body is exposed in an environment with the relative humidity of 10-30% for 30min, the water content of the horny layer is obviously reduced; the dry environment reduces the water content of the horny layer of the epidermis, and the low air humidity can inhibit the synthesis of NMF (N-methyl-pyrrolidone) by filaggrin in the horny layer. Therefore, in a dry environment, the moisture content of the skin is obviously reduced, the skin becomes dry, the closed humectant cannot effectively prevent the evaporation of the moisture of the skin, and the moisture in the dry environment is less, so that the moisture-keeping effect cannot be achieved by absorbing the moisture of the external environment or hydrating the moisture with the moisture in the external environment, and the loss of the moisture cannot be effectively prevented due to the problem that the horny layer of most people is damaged at present, which is particularly obvious in the dry environment. Therefore, the current moisturizing cosmetics cannot guarantee effective moisturizing in a dry environment.
Post-inflammatory pigmentation is a rare disease state of skin hyperpigmentation, which is mainly related to oxidative stress and inflammatory mediators and cytokine invasion, and currently, corticosteroids are generally combined with photoprotectants to control inflammation so as to inhibit further deposition of melanin, but the treatment course is long; secondly, inhibitors with tyrosinase activity such as azelaic acid and kojic acid are mostly adopted to treat the pigmentation after inflammation, but most clinical application experiments show that the inhibitors can obviously increase the visual difference between the pigmentation part after inflammation and other parts, and have no obvious effect on clearing the melanin in the pigmentation part, because the tyrosinase inhibitors have different action parts and generally act on the surface layer of the skin, the inhibitors have better clearing effect on the conventional melanin and obvious whitening effect, but the melanin generated after inflammation is mainly deposited on the dermis, the inhibitors cannot generate the effect in a short time, and the side effect of the skin generated by the inhibitors is also common.
Therefore, there is a need for a cosmetic formulation that addresses post-inflammatory pigmentation and has the effect of moisturizing the skin in a dry environment.
Disclosure of Invention
The invention aims to overcome the defects of the existing product and provide a skin care composition which aims at repairing the pigmentation after inflammation and has the deep instant moisturizing effect.
The above purpose of the invention is realized by the following technical scheme: the skin care composition for whitening and moisturizing comprises the following components in parts by weight: 20-35 parts of a spider-web inula herb extract, 5-22 parts of a carrageen crispus extract, 1-5 parts of a hypecoum extract and 3-12 parts of a sea buckthorn fruit extract.
Preferably, the skin care composition for whitening and moisturizing comprises the following components in parts by weight: 22 parts of arachnoid bluegrass extract, 15 parts of carrageen crispus extract, 3 parts of hypecoum vulgare extract and 6 parts of sea buckthorn fruit extract.
Preferably, the arachnoid palustris extract is obtained by extracting the arachnoid palustris 1% methanol hydrochloride extract serving as a raw material with ethyl acetate.
Preferably, the preparation method of the arachnoid giantrum herb extract comprises the following steps:
s1, taking dry arachnoid nepenthes, cleaning, crushing, adding 1% hydrochloric acid methanol solution according to the material-liquid ratio of 1: 2-6 for extraction, filtering, and concentrating under reduced pressure to obtain the 1% hydrochloric acid methanol extract of arachnoid nepenthes;
s2, taking the 1% methanol hydrochloride extract of the arachnoid nepalensis, and adding deionized water to dilute the extract to a relative density of 1.0-1.2 g/cm3Adding ethyl acetate of which the volume is 1/2-1 times that of the suspension into the suspension, extracting for 1-3 times, filtering, combining filtrates, and concentrating under reduced pressure to obtain the arachnoid nepenthes ethyl acetate extract of the 1% hydrochloric acid methanol extract.
Preferably, the extraction temperature in the step S1 is 65-90 ℃; the extraction times are 1-3 times; the ratio of the materials to the liquid is 1: 3-5. More preferably, the extraction temperature is 80 ℃, 65 ℃, 75 ℃ or 90 ℃, more preferably 80 ℃; the extraction times are preferably 2 times; the feed-liquid ratio is preferably 1: 3.2.
Preferably, the Chondrus crispus extract is 50-75% ethanol extract of Chondrus crispus. The preparation method comprises the following steps: taking the carrageen crispus, cleaning, drying, grinding into fine powder, adding 50-75% by volume of ethanol solution according to the material-liquid ratio of 1g to 10-15 ml, extracting for 1-3 times at 75-85 ℃, filtering, combining filtrates, and concentrating under reduced pressure to obtain the carrageen crispus extract.
Preferably, the seabuckthorn fruit extract is obtained by heating and reflux extraction with 50-60% ethanol as an extraction solvent. The specific preparation method comprises the following steps: taking and crushing the tissues of the sea buckthorn fruits, adding 50-60% ethanol solution according to the material-liquid ratio of 1g to 12-20 ml, extracting for 1-3 times at 50-70 ℃, filtering, combining filtrates, and concentrating under reduced pressure to obtain the sea buckthorn fruit extract.
The invention also aims to provide application of the skin care composition in preparing cosmetics with whitening and moisturizing effects.
The invention also aims to provide a cosmetic preparation with whitening and moisturizing effects, which contains 1-99.99% of the skin care composition.
Herein, "whitening" is different from the conventional meaning of whitening, and the emphasis herein is on whitening, in terms of post-inflammatory pigmentation, which does not have an action mechanism in pursuit of inhibition of tyrosinase activity, since it has been confirmed that inhibition of tyrosinase activity alone is not effective in curing post-inflammatory pigmentation.
Herein, "post-inflammatory pigmentation" is derived from atopic dermatitis, allergic contact dermatitis, acne or skin infection, and is particularly effective for being derived from atopic dermatitis and allergic contact dermatitis.
Preferably, the weight of the skin care composition in the cosmetic preparation is 5-32%.
In addition, cosmetically acceptable excipients may be included in the cosmetic formulations of the present invention, including but not limited to: thickening agent, emulsifier, chelating agent, emollient, grease, preservative, colorant, emulsion stabilizer, etc.
The skin care essence with whitening and moisturizing effects comprises the following components in percentage by mass: 0.1-0.3% of thickening agent, 1-3% of emulsifying agent, 1-6% of emollient, 5-32% of skin care composition, 0.1-0.2% of preservative, 0.01-0.03% of chelating agent and the balance of deionized water.
The invention has the following beneficial effects:
(1) the ethyl acetate extract of the alcohol extract of the arachnoid bluegrass cooperates with the carrageen crispus extract, the hypsizygus marmoreus extract and the seabuckthorn fruit extract to serve as the functional components, and the skin care composition can fade the pigment of a dermis layer by repairing internal inflammation of skin, so that the purpose of effectively relieving the pigmentation after facial inflammation is achieved, skin aging can be prevented, and the skin can be white and fine.
(2) In addition, the skin care composition can enhance the strength and the appearance of the corneocytes and enable the shape of the corneocytes to be more complete and compact through the combined action of the four effective components, thereby effectively avoiding water loss and improving the water content of the skin from the source under the low-humidity environment; on the other hand, the moisture in the environment can be absorbed to the maximum extent, and the instant and lasting moisture preservation effect in normal environment and dry environment is realized through water capture, water locking and water storage.
Detailed Description
The present invention is further illustrated by the following specific examples, which are not intended to limit the invention in any way. Reagents, methods and apparatus used in the present invention are conventional in the art unless otherwise indicated.
Unless otherwise indicated, reagents and materials used in the following examples are commercially available.
Example 1, composition 1
Preparation of an extract of arachnoid bluegrass:
s1, taking dry arachnoid nepenthes, cleaning, crushing, adding 1% methanol hydrochloride solution according to the material-to-liquid ratio of 1:3.2, extracting for 2 times at the temperature of 80 ℃, filtering, and concentrating the filtrate under reduced pressure to obtain the 1% methanol hydrochloride extract of arachnoid nepenthes;
s2, adding deionized water into the 1% methanol hydrochloride extract of the above herba Centellae, and diluting to relative density of 1.0g/cm3Adding 1/2 times volume of ethyl acetate into the suspension, extracting for 2 times, filtering, combining the filtrates, and concentrating under reduced pressure to obtain the extract of the arachnoid's Lancet 1% methanol hydrochloride extract.
Preparation of Chondrus crispus extract:
cleaning Chondrus crispus, drying, grinding into fine powder, adding 65% ethanol solution according to the material-liquid ratio of 1g to 12ml, extracting at 80 deg.C for 3 times, filtering, mixing filtrates, and concentrating under reduced pressure to obtain the Chondrus crispus extract.
Preparing a seabuckthorn fruit extract:
taking seabuckthorn fruits, crushing tissues, adding 50% ethanol solution according to the material-liquid ratio of 1g to 15ml, extracting for 2 times at 60 ℃, filtering, combining filtrates, and concentrating under reduced pressure to obtain the seabuckthorn fruit extract.
The composition of this example consisted of 22 parts of extracts of arachnoid bluegrass, 15 parts of extracts of carrageen crispus, 3 parts of extracts of hypecoum vulgare and 6 parts of extracts of hippophae rhamnoides.
Example 2, composition 2
Preparation of an extract of arachnoid bluegrass:
s1, cleaning and crushing dry arachnoid palum herb, adding 1% methanol hydrochloride solution according to the material-to-liquid ratio of 1:2, extracting for 3 times at 65 ℃, filtering, and concentrating under reduced pressure to obtain the 1% methanol hydrochloride extract of arachnoid palum herb;
s2, adding deionized water into the 1% methanol hydrochloride extract of the above herba Centellae, and diluting to relative density of 1.2g/cm3Adding 1/3 times volume of ethyl acetate into the suspension, extracting for 3 times, filtering, combining the filtrates, and concentrating under reduced pressure to obtain the extract of the arachnoid's Lancet 1% methanol hydrochloride extract.
Preparation of Chondrus crispus extract:
cleaning Chondrus crispus, drying, grinding into fine powder, adding 50% ethanol solution according to the material-liquid ratio of 1g to 10ml, extracting at 75 deg.C for 2 times, filtering, mixing filtrates, and concentrating under reduced pressure to obtain the Chondrus crispus extract.
Preparing a seabuckthorn fruit extract:
taking seabuckthorn fruits, crushing tissues, adding 55% ethanol solution according to the material-liquid ratio of 1g to 12ml, extracting for 3 times at 55 ℃, filtering, combining filtrates, and concentrating under reduced pressure to obtain the seabuckthorn fruit extract.
The composition of this example consisted of 20 parts of extracts of arachnoid bluegrass, 10 parts of extracts of carrageen crispus, 1 part of extracts of hypecoum vulgare and 5 parts of extracts of hippophae rhamnoides.
Example 3, composition 3
Preparation of an extract of arachnoid bluegrass:
s1, cleaning and crushing dry arachnoid palum herb, adding 1% methanol hydrochloride solution according to the material-to-liquid ratio of 1:5, extracting for 3 times at the temperature of 75 ℃, filtering, and concentrating under reduced pressure to obtain the 1% methanol hydrochloride extract of arachnoid palum herb;
s2, adding deionized water into the 1% methanol hydrochloride extract of the above herba Centellae, and diluting to relative density of 1.1g/cm3Adding 1/2 times volume of ethyl acetate into the suspension, extracting for 3 times, filtering, combining the filtrates, and concentrating under reduced pressure to obtain the extract of the arachnoid's Lancet 1% methanol hydrochloride extract.
Preparation of Chondrus crispus extract:
cleaning Chondrus crispus, drying, grinding into fine powder, adding 55% ethanol solution according to the material-liquid ratio of 1g to 15ml, extracting at 85 deg.C for 2 times, filtering, mixing filtrates, and concentrating under reduced pressure to obtain the Chondrus crispus extract.
Preparing a seabuckthorn fruit extract:
pulverizing fructus Hippophae, adding 60% ethanol solution according to a material-liquid ratio of 1g:20ml, extracting at 70 deg.C for 3 times, filtering, mixing filtrates, and concentrating under reduced pressure to obtain the fructus Hippophae extract.
The composition of this example consisted of 32 parts of extracts of arachnoid bluegrass, 12 parts of extracts of carrageen crispus, 2 parts of extracts of hypecoum vulgare and 8 parts of extracts of hippophae rhamnoides.
Examples 4 to 6 skin care essence and mass fraction thereof
Figure BDA0002728895920000051
Figure BDA0002728895920000061
Note: the skin care compositions of examples 4-6 correspond to the skin care compositions prepared in examples 1-3, respectively.
The preparation method comprises the following steps:
putting deionized water with formula amount into a water phase pot, slowly adding carbomer while stirring, slowly adding ethylene glycol and sodium di (lauramide glutamine) lysine after uniform dispersion, heating and stirring to 80 deg.C, keeping the temperature for 3min, and filtering; adding cetearyl alcohol, cetearyl glucoside, polydimethylsiloxane cross-linked polymer, titanium dioxide and hydrogenated lecithin into an oil phase pot, starting stirring, heating to 80 ℃, uniformly stirring, and filtering; pumping the water phase material into a vacuum emulsifying pot, slowly adding the oil phase material, stirring, and homogenizing for 5 min; cooling to 45 deg.C, adding skin care composition, and stirring; cooling to 35 deg.C, adding phenoxyethanol and benzoic acid, stirring, inspecting, discharging, and packaging.
Comparative example 1 compared to example 4, except that the skin care composition was only an extract of Echinacea purpurea, the remaining parameters were the same as in example 4.
Comparative example 2, compared to example 4, with the difference that the skin care composition was solely the extract of Chondrus crispus, the remaining parameters were the same as in example 4.
Comparative example 3 compared to example 4, except that the skin care composition was solely anise extract, the remaining parameters were the same as in example 4.
Comparative example 4 compared to example 4, except that the skin care composition was only an extract of hippophae rhamnoides fruit, the remaining parameters were the same as in example 4.
Comparative example 5, compared with example 4, differs in that the extraction step with ethyl acetate is omitted from the preparation of the extract of the spider-web-like Lancete, and the remaining parameters are the same as in example 4.
Test example I Water replenishment test under different environments
Test samples: the essence prepared in examples 4 to 6 and comparative examples 1 to 5.
The test method comprises the following steps: 160 subjects were selected, the average age was 32 ± 1 year, and the subjects were randomly divided into 8 groups, each group consisting of 20 subjects, and each group of subjects could not use any external drugs or cosmetics for 1 week before the test, each group of patients cleaned their faces, stood still for 30min under different humidity conditions, skin moisture content values of the blank areas before the use of the test sites were measured using a skin moisture meter, then the test samples were applied to the facial skin, an appropriate amount of skin care product was applied to the skin during the use, massaged until the absorption, skin moisture contents were measured at different times after the use using a skin moisture meter, data of all the test sites of all the volunteers of the same example or comparative example were averaged, and skin moisture content variation values were calculated, with the results shown in tables 1 and 2 below.
Figure BDA0002728895920000071
In the formula, MMV0MMV for use with skin; MMVtMMV for skin at different time t after use.
TABLE 1 variation of skin moisture content in normal environment (temperature 23 + -2 deg.C, humidity 50 + -5%)
Figure BDA0002728895920000072
TABLE 2 variation of skin moisture content in dry environment (temperature 15. + -. 1 ℃ C., humidity 20. + -. 5%)
Figure BDA0002728895920000073
Figure BDA0002728895920000081
The above table shows that the skin care essence liquid of the embodiments 4-6 can provide sufficient moisture for the skin in normal environment and dry environment, the moisture of the skin is obviously increased after the skin care essence liquid is used for 4 hours, the moisture of the skin reaches a peak value at 8H, and then the moisture of the skin is slightly reduced, but the moisture change value is still maintained at a higher level, and the skin care essence liquid is proved to be capable of meeting the instantaneous moisture preservation requirements of the skin in normal and dry environments; however, if the active ingredients were separately removed and applied to the skin, it was found that the moisturizing effect comparable to examples 4 to 6 could not be maintained, which shows the synergistic effect between the respective ingredients.
Test example two, test for improvement of post-inflammatory pigmentation
80 volunteers of post-inflammatory hyperpigmentation due to acne were selected and randomly divided into 8 groups of 10 individuals, each group of subjects was subjected to face cleansing in the morning and evening using the products of examples 4 to 6 and comparative examples 1 to 5 for 4 weeks, skin whiteness (brightness) (L value) and skin brightness (dark yellowness) (ITA DEG value) of the face of the subjects before and after use at 1 week and 4 week were measured 3 times per spot using a skin color difference test probe, and the results were shown in Table 3.
TABLE 3 skin Brightness and Brightness variation
Figure BDA0002728895920000082
Figure BDA0002728895920000091
Note: percent change (%) - (after use-before use)/100% before use
The above table shows that the four ingredients can effectively improve the whiteness and brightness of facial skin in a synergistic manner, and the whitening and brightness enhancing composition has a remarkable effect on post-inflammatory pigmentation; it is also noted that the effect of treatment with the hypecoum vulgare extract alone, which has tyrosinase activity inhibiting effect, is significantly reduced compared to example 1, indicating that the problem of post-inflammatory pigmentation cannot be effectively improved by means of tyrosinase activity inhibition alone.
The above embodiments are preferred embodiments of the present invention, but the present invention is not limited to the above embodiments, and any other changes, modifications, substitutions, combinations, and simplifications which do not depart from the spirit and principle of the present invention should be construed as equivalents thereof, and all such changes, modifications, substitutions, combinations, and simplifications are intended to be included in the scope of the present invention.

Claims (10)

1. The skin care composition for whitening and moisturizing is characterized by comprising the following components in parts by weight: 20-35 parts of a spider-web inula herb extract, 5-22 parts of a carrageen crispus extract, 1-5 parts of a hypecoum extract and 3-12 parts of a sea buckthorn fruit extract.
2. The skin care composition for whitening and moisturizing according to claim 1, which comprises the following components in parts by weight: 22 parts of arachnoid bluegrass extract, 15 parts of carrageen crispus extract, 3 parts of hypecoum vulgare extract and 6 parts of sea buckthorn fruit extract.
3. The skin care composition for whitening and moisturizing according to claim 1 or 2, wherein the extract of the arachnoid component is obtained by extracting the extract of the arachnoid component with 1% methanol hydrochloride of the arachnoid component with ethyl acetate.
4. The skin care composition for whitening and moisturizing according to claim 3, wherein the extract of the arachnoid bluegrass is prepared by a method comprising:
s1, taking dry arachnoid nepenthes, cleaning, crushing, adding 1% hydrochloric acid methanol solution according to the material-liquid ratio of 1: 2-6 for extraction, filtering, and concentrating under reduced pressure to obtain the 1% hydrochloric acid methanol extract of arachnoid nepenthes;
s2, taking the 1% methanol hydrochloride extract of the arachnoid nepalensis, and adding deionized water to dilute the extract to a relative density of 1.0-1.2 g/cm3Adding ethyl acetate of which the volume is 1/2-1 times that of the suspension into the suspension, extracting for 1-3 times, filtering, combining filtrates, and concentrating under reduced pressure to obtain the arachnoid nepenthes ethyl acetate extract of the 1% hydrochloric acid methanol extract.
5. The skin care composition for whitening and moisturizing according to claim 4, wherein the extraction temperature in the step S1 is 65-90 ℃; the extraction times are 1-3 times; the ratio of the materials to the liquid is 1: 3-5.
6. The skin care composition for whitening and moisturizing according to claim 1 or 2, wherein the carrageen crispus extract is 50-75% ethanol extract of carrageen crispus.
7. The skin care composition for whitening and moisturizing according to claim 1 or 2, wherein the seabuckthorn fruit extract is obtained by heating and reflux extraction with 50-60% ethanol as an extraction solvent.
8. Use of the skin care composition according to any one of claims 1 to 7 in the preparation of cosmetics with whitening and moisturizing effects.
9. A cosmetic preparation having whitening and moisturizing effects, characterized by comprising 1 to 99.99% of the skin care composition according to any one of claims 1 to 7.
10. The cosmetic formulation of claim 9, wherein the skin care composition is present in the cosmetic formulation in an amount of from 5% to 32% by weight.
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